BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,257,488	A→C	15.9%	V293G (GTG→GGG)	psuG ←	pseudouridine 5'‑phosphate glycosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,257,488	0	A	C	15.9%	304.9 / 5.4	116	V293G (GTG→GGG)	psuG	pseudouridine 5'‑phosphate glycosidase
Reads supporting (aligned to +/- strand): ref base A (67/25); new base C (2/21); total (69/47)
Fisher's exact test for biased strand distribution p-value = 1.85e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 117 total aligned reads displayed.

TTTTATTCACTGACAACAGAAATGCTATTGCCAGTAACACCACCATCCCCACAACACTTCTCATTATATCCATAATGATTTTCCCTT‑CATGCCGGTAAACCCGGCGTCAGCGCCAGGTTTTGGTATGCTTGATGAGTACGGG‑CG‑ACGGCTTTCTGCCCGTCAGAAATTAACCCGCGAGACG‑CTGATATTCTTTGGCAATTT‑CGCTCGCCAGAATGGC‑GTTGTTGAACACCAGCTGGATGTTGGATTTCAGGCTGTCACCGCCGGTCAGTTCAGCAACGCGCGCCAGCAGGAATGGCGTACTTTCTTTACCAATGACTCCCTGAGCTTCAGCTTCAGCTACCGCCTGATCGATCGCCGCATTGATAGTGTGTTCCGGCATAGCAAACTGTTCCGGGATCGGGTTCGCTACCACGAGGCCACCGTTCAGACCGCTTTGCCATTTCACCACCATTGCACGGGCAAT  >  NC_000913/2257261‑2257723

ttttATTCACTGACAACAGAAATGCTATTGCCAGTAACACCACCATCCCCACAACACTTCTCATTATATCCATAATGATTTTCCCTT‑CATGCCGGTAAACCCGGCGTCAGCGCCAGGTTTTGGTATGCTTGATGAGTACGGG‑CG‑ACGGCTTTCTGCCCGTCAGAAATTAACCCGCGAGACG‑CTGATATTCTTTGGCAATTT‑CGCTCGCCAGAATGGC‑GTTGTTGAACACCAGCTGGATg                                                                                                                                                                                                                                  <  2:927579/239‑1 (MQ=255)

  ttATTCACTGACAACAGAAATGCTATTGCCAGTAACACCACCATCCCCACAACACTTCTCATTATATCCATAATGATTTTCCCTT‑CATGCCGGTAAACCCGGCGTCAGCGCCAGGTTTTGGTATGCTTGATGAGTACGGG‑CG‑ACGGCTTTCTGCCCGTCAGAAATTAACCCGCGAGACG‑CTGATATTCTTTGGCAATTT‑CGCTCGCCAGAATGGC‑GTTGTTGAACACCAGCTGGATGtt                                                                                                                                                                                                                                <  1:177267/239‑1 (MQ=255)

         cTGACAACAGAAATGCTATTGCCAGTAACACCACCATCCCCACAACACTTCTCATTATATCCATAATGATTTTCCCTT‑CATGCCGGTAAACCCGGCGTCAGCGCCAGGTTTTGGTATGCTTGATGAGTACGGG‑CG‑ACGGCTTTCTGCCCGTCAGAAATTAACCCGCGAGACG‑CTGATATTCTTTGGCAATTT‑CGCTCGCCAGAATGGC‑GTTGTTGAACACCAGCTGGATGTTGGATTTc                                                                                                                                                                                                                         >  2:193725/1‑239 (MQ=255)

         cTGACAACAGAAATGCTATTGCCAGTAACACCACCATCCCCACAACACTTCTCATTATATCCATAATGATTTTCCCTT‑CATGCCGGTAAACCCGGCGTCAGCGCCAGGTTTTGGTATGCTTGATGAGTACGGG‑CG‑ACGGCTTTCTGCCCGTCAGAAATTAACCCGCGAGACG‑CTGATATTCTTTGGCAATTT‑CGCTCGCCAGAATGGC‑GTTGTTGAACACCAGCTGGATGTTGGATTTc                                                                                                                                                                                                                         >  1:848659/1‑239 (MQ=255)

                          aggaccatcaaCACCCCCACCCCCACAACCCTTCTCTTTATACCCATAATGTGTTTCCCTC‑CATGCCGGTACACCCGGCGTCAGCGCCAGGTTTTGGTATGCTTGATGAGTACGGG‑CG‑ACGGCTTTCTGCCCGTAAAAAATTAACCCGCGAGACG‑CTGATATTCTTTGGCAATTT‑CGCTCGCCAGAATGGC‑GTTGTTGAACCCCAGCTGGATGTTGGTTTTCAGGCTGTCACCGCCGGt                                                                                                                                                                                                        <  2:704586/230‑1 (MQ=255)

                          aTTGCCAGTAACACCACTATCCCCACAACACATCTCATTATATCCATAATGATTTTCCCTT‑CATGCCGGTAAACCCGGCGTCACCGCCAGGTTTTGGTTTGCTTGATGAGTACGGG‑CG‑ACGGCTTCCTGCCCGTCAGAAATTACCCCGCGAGACG‑CTGATATCCTCTGGCAATTT‑CGCTCGCCAGAATCGC‑GCTGTTGCACACAAGCTGGATGTTGGATTTCAGGCTGTAACCGCCgg                                                                                                                                                                                                         >  2:699974/1‑238 (MQ=255)

                             gCCAGTAACACCACCATCCCCACAACACTTCTCATTATATCCATAATGATTTTCCCTT‑CATGCCGGTAAACCCGGCGTCAGCGCCAGGTTTTGGTATGCTTGATGAGTACGGG‑CG‑ACGGCTTTCTGCCCGTCAGAAATTAACCCGCGAGACG‑CTGATATTCTTTGGCAATTT‑CGCTCGCCAGAATGGC‑GGTGTTGAACACCAGCTGGATGTTGGATTTCAGGCTGTCACCGCCGGTCAg                                                                                                                                                                                                     >  2:66157/1‑239 (MQ=255)

                             gCCAGTAACACCACCATCCCCACAACACTTCTCATTATATCCATAATGATTTTCCCTT‑CATGCCGGTAAACCCGGCGTCAGCACCAGGTTTTGGTATGCTTGATGAGTACGGG‑CG‑ACAGCTTTCTGCCCGTCAGAAATTAACCCGCGAGACG‑CTGATATTCTTTGGCAATTT‑CGCTCGCCAGAATGGC‑GTTGTTGAACACCAGCTGGATGTTGGATTTCAGGCTGTCACCGCCGGTCAg                                                                                                                                                                                                     >  1:1350505/1‑239 (MQ=255)

                                aGTAACACCACCATCCCCACAACACTTCTCATTATATCCATAATGATTTTCCCTT‑CATGCCGGTAAACCCGGCGTCAGCGCCAGGTTTTGGTATGCTTGATGAGTACGGG‑CG‑ACGGCTTTCTGCCCGTCAGAAATTAACCCGCGAGACG‑CTGATATTCTTTGGCAATTT‑CGCTAGCCAGAATGGC‑GTTGTTGAACACCAGCTGGATGTTGGATTTCAGGCTGTCACCGCCGGTCAGTTc                                                                                                                                                                                                  >  2:560171/1‑239 (MQ=255)

                                 gTAACACCACCATCCCCACAACACTTCTCATTATATCCATAATGATTTTCCCTT‑CATGCCGGTAAACCCGGCGTCAGCGCCAGGTTTTGGTATGCTTGATGAGTACGGG‑CG‑ACGGCTTTCTGCCCGTCAGAAATTAACCCGCGAGACG‑CTGATATTCTTTGGCAATTT‑CGCTCGCCAGAATGGC‑GTTGTTGAACACCAGCTGGATGTTGGATTTCAGGCTGTCACCGCCGGTCAGTTCa                                                                                                                                                                                                 >  1:1180941/1‑239 (MQ=255)

                                  tAACACCACCATCCCCACAACACTTCTCATTATATCCATAATGATTTTCCCTT‑CATGCCGGTAAACCCGGCGTCAGCGCCAGGTTTTGGTATGCTTGATGAGTACGGG‑CG‑ACGGCTTTCTGCCCGTCAGAAATTAACCCGCGAGACG‑CTGATATTCTTTGGCAATTT‑CGCTCGCCAGAATGGC‑GTTGTTGAACACCAGCTGGATGTTGGATTTCAGGCTGTCACCGCCGGTCAGTTCa                                                                                                                                                                                                 >  1:212427/1‑238 (MQ=255)

                                             tCCCCACAACACTTCTCATTATATCCATAATGATTTTCCCTT‑CATGCCGGTAAACCCGGCGTCAGCCCCAGGTTTTGGTATGCTTGATGAGTACGGG‑CG‑ACGGCTTTCTGCCCGTCAGAAATTAACCCGCGAGACG‑CTGATATTCTTTGGCAATTT‑CGCTCGCCAGAATGGC‑GTTGTTGAACACCAGCTGGATGTTGGATTTCAGGCTGTCACCGCCGGTCAGTTCAGCAACGCGCGcc                                                                                                                                                                                     >  2:798474/1‑239 (MQ=255)

                                               ccccCAACACTTCTAATTATACCCATAATGATTTTCCCTT‑CATGCCGGTAAACCCGGCGTCAGCGCCAGTTTTTGGTAGGCTTGATGAGTCCGGG‑CG‑ACGGCTTTCTGCCCGTAAGAAATTACCCCGCGAGACG‑CTGATATTTTTTGGCAATTT‑CGCTCGCCAGAAGGGC‑GTTGTTGAACACCAGCTGGATGTTGGATTTCAGGCTGTCACCGCCGGTCAGTTCAGCAACGCGCGCcag                                                                                                                                                                                   <  2:1288179/239‑1 (MQ=255)

                                                  acaacaCTTCTCATTATATCCATAATGATTTTCCCTT‑CATGCCGGTAAACCCGGCGTCAGCGCCAGGTTTTGGTATGCTTGATGAGTACGGG‑CG‑ACGGCTTTCTGCCCGTCAGAAATTAACCCGCGAGACG‑CTGATATTCTTTGGCAATTT‑CGCTCGCCAGAATGGC‑GTTGTTGAACACCAGCTGGATGTTGGATTTCAGGCTGTCACCGCCGGTCAGTTCAGCAACGCGCGCcagcag                                                                                                                                                                                >  1:1288179/1‑239 (MQ=255)

                                                      accatctaATAATATCCATAATG‑TTTTCCCTTACACGCCGGTAAACCGGGCGTAGGCGCCAGTTTTTGGTATGCTTGATGAGTACGGG‑CG‑ACGGCTTTCTGCCCGTCAGAAATTACCCGGGGAGACG‑CTGATATTCTTTGGCAATTT‑CGCTCGCCAGAATGGC‑GTTGTTGAACACCAGCGGGATGTTGGATTTCAGGCTGTCACCGCCGGTCAGTTCAGCAACGCGCGCCAGCAGGAAt                                                                                                                                                                            <  2:382412/235‑1 (MQ=255)

                                                         ttCTCATTATATCCATAATGATTTTCCCTT‑CATGCCGGTAAACCCGGCGTCAGCGCCAGGTTTTGGTATGCTTGATGAGTACGGG‑CG‑ACGGCTTTCTGCCCGTCAGAAATTAACCCGCGAGACG‑CTGATATTCTTTGGCAATTT‑CGCTCGCCAGAATGGC‑GTTGTTGAACACCAGCTGGATGTTGGATTTCAGGCTGTCACCGCCGGTCAGTTCAGCAACGCGCGCCAGCAGGAATGGc                                                                                                                                                                         >  2:684111/1‑239 (MQ=255)

                                                           ctcATTATATCCATAATGATTTTCCCTT‑CATGCCGGTAAACCCGGCGTCAGCGCCAGGCTTTGGTATGCTTGATGAGTACGGG‑CG‑ACGGCTTTCTGCCCGTCAGAAATTAACCCGCGAGACG‑CTGATATTCTTTGGCACTTT‑CGCTCGCCAGAATGGC‑GTTGTTGAACCCCAGCTGGATGTTGGATTTCAGGCTGTCACCGCCGGTCAGTTCAGCAACGCGCGCCAGCAggca                                                                                                                                                                             >  1:369081/1‑231 (MQ=255)

                                                              aTTATATCCATAATGATTTTCCCTT‑CATGCCGGTAAACCCGGCGTCAGCGCCAGGTTTTGGTATGCTTGATGAGTACGGG‑CG‑ACGGCTTTCTGCCCGTCAGAAATTAACCCGCGAGACG‑CTGATATTCTTTGGCAATTT‑CGCTCGCCAGAATGGC‑GTTGTTGAACACCAGCTGGATGTTGGATTTCAGGCTGTCACCGCCGGTCAGTTCAGCAACGCGCGCCAGCAGGAATGGCGTAct                                                                                                                                                                    >  1:840502/1‑239 (MQ=255)

                                                              aTTATATCCATAATGATTTTCCCTT‑CATGCCGGTAAACCCGGCGTCAGCGCCAGGTTTTGGTATGCTTGATGAGTACGGG‑CG‑ACGGCTTTCTGCCCGTCAGAAATTAACCCGCGAGACG‑CTGATATTCTTTGGCAATTT‑CGCTCGCCAGAATGGC‑GTTGTTGAACACCAGCTGGATGTTGGATTTCAGGCTGTCACCGCCGGTCAGTTCAGCAACGCGCGCCAGCAGGAATGGCGTAct                                                                                                                                                                    >  2:1044839/1‑239 (MQ=255)

                                                                tataTCCATAATGATTTTCCCTT‑CATGCCGGTAAACCCGGCGTCAGCGCCAGGTTTTGGTATGCTTGATGAGTACGGG‑CG‑ACGGCTTTCTGCCCGTCAGAAATTAACCCGCGAGACG‑CTGATATTCTTTGGCAATTT‑CGCTCGCCAGAATGGC‑GTTGTTGAACACCAGCTGGATGTTGGATTTCAGGCTGTCACCGCCGGTCAGTTCAGCAACGCGCGCCAGCAGGAATGGCGTActt                                                                                                                                                                   >  1:1100122/1‑238 (MQ=255)

                                                                tataTCCATAATGATTTTCCCTT‑CATGCCGGTAAACCCGGCGTCAGCGCCAGGTTTTGGTATGCTTGATGAGTACGGG‑CG‑ACGGCTTTCTGCCCGTCAGAAATTAACCCGCGAGACG‑CTGATATTCTTTGGCAATTT‑CGCTCGCCAGAATGGC‑GTTGTTGAACACCAGCTGGATGTTGGATTTCAGGCTGTCACCGCCGGTCAGTTCAGCAACGCGCGCCAGCAGGAATGGCGTActt                                                                                                                                                                   >  1:688853/1‑238 (MQ=255)

                                                                  tatCAATAATGATTTTCCCTT‑CATGCCGGTAAACCCGGCGCAAGCGCCAGTTTTTGGTATGCTTGAGGAGTACGGG‑CG‑ACGGCTTTCTGCCCGTCAGAAATTACCCCGCGAGACG‑CTGATATTCTTTGGCAATTT‑CGCTCGCCAGAATGGC‑GTTGTTGAACCCCAGCTGGATGTTGGATTTCAGGCGGTCACCGCCGGTCAGTTCAGCAACGCGCGCCAGCAGGAATGGCGTActttct                                                                                                                                                                <  2:334849/239‑1 (MQ=255)

                                                                   atCCATAATGATTTTCCCTT‑CATGCCGGTAAACCCGGCGTCAGCGCCAGGTTTTGGTATGCTTGATGAGTACGGG‑CG‑ACGGCTTTCTGCCCGTCAGAAATTAACCCGCGAGACG‑CTGATATTCTTTGGCAATTT‑CGCTCGCCAGAATGGC‑GTTGTTGAACACCAGCTGGATGTTGGCTTTCAGGCTGTCACCGCCGGTCAGTTCAGCAACGCGCGCCAGCAGGAATGGCGTActttctt                                                                                                                                                               >  1:470695/1‑239 (MQ=255)

                                                                   atCCATAATGATTTTCCCTT‑CATGCCGGTAAACCCGGCGTCAGCGCCAGGTTTTGGTATGCTTGATGAGTACGGG‑CG‑ACGGCTTTCTGCCCGTCAGAAATTAACCCGCGAGACG‑CTGATATTCTTTGGCAATTT‑CGCTCGCCAGAATGGC‑GTTGTTGAACACCAGCTGGATGTTGGATTTCAGGCTGTCACCGCCGGTCAGTTCAGCAACGCGCGCCAGCAGGAATGGCGTActttctt                                                                                                                                                               >  1:790812/1‑239 (MQ=255)

                                                                    ccaaaaaTGATTTCCCTTC‑CACGCGGGTAACCCCGGCGTCAGGCCCAGTTTTTGGTATGCTGGATGAGTACGGG‑CG‑ACGGCTTTCTGCCCGTCAGAAATTAACCCGCGAGACG‑CTGATATTCTTTGGCAATTT‑CGCTCGCCAGAATGGC‑GTTGTTGAACACCAGCTGGATGTTGGATTTCAGGCTGTCACCGCCGGTCAGTTCAGCAACGCGCGCCAGCAGGAATGGCGTActttcttt                                                                                                                                                              <  2:688853/234‑1 (MQ=255)

                                                                    catcaatggATTTCCCCTT‑CAGCCCGGTAACCCGGGCTCCCGCCCCAGTTTTTGGTATGCTGGATGAGTCCGGG‑CG‑GCGGCTTTCTGCCCGTCAGAAATTACCCCGCGGGACG‑CTAATATTCTTTGGCATTTT‑CGCCCGCAAGAATGGC‑GTTGTTAAAACCCAGCGGGATGTGGGATTTCAGGCTGTCACCGCCGGTCAGTTCAGCAACGCGCGCCAGCAGGAATGGCGTActttcttt                                                                                                                                                              <  2:1016625/231‑1 (MQ=255)

                                                                        tAATGATTTTCCCTT‑CATGCCGGTAAACCCGGCGTCAGCGCCAGGTTTTGGTATGCTTGATGAGTGCGGG‑CG‑ACGGCTTTCTGCCCGTCAGAAATTAACCCGCGCGACG‑CTGATCTTCTTTGTCAATTT‑CGCGCGCCAGCATGGC‑GTTGTTGAACCACAGCTGGATGCTGGATTTCAGGCTGTCACCGCCGGTCAGTTCAGCAACGCGCGCCAGCAGGCATGGTGTACTTTCTTTAcc                                                                                                                                                           >  2:629537/1‑238 (MQ=255)

                                                                        tAATGATTTTCCCTT‑CATGCCGGTAAACCCGGCGTCAGCGCCAGGTTTTGGTATGCTTGATGAGTACGGG‑CG‑ACGGCTTTCTGCCCGTCAGAAATTAACCCGCGAGACG‑CTGATATTCTTTGGCAATTT‑CGCTCGCCAGAATGGC‑GTTGTTGAACACCAGCTGGATGTTGGATTTCAGGCTGTCACCGCCGGTCAGTTCAGCAACGCGCGCCAGCAGGAATGGCGTACTTTCTTTACCa                                                                                                                                                          >  2:515053/1‑239 (MQ=255)

                                                                         aataatTTTCCCTT‑CATGCCGGTAAACCCGGCGTCAGCGCCAGGTTTTGGTATGCTTGATGAGTACGGG‑CG‑ACGGCTTTCTGCCCGTCAGAAATTAACCCGCGAGACG‑CTGATATTCTTTGGCAATTT‑CGCTCGCCAGAATGGC‑GTTGTTGAACACCAGCTGGATGTTGGATTTCAGGCTGTCACCGCCGGTCAGTTCAGCAACGCGCGCCAGCAGGAATGGCGTACTTTCTTTACCaa                                                                                                                                                         >  1:568376/1‑239 (MQ=255)

                                                                         aaTGATTTTCCCTT‑CATGCCGGTAAACCCGGCGTCAGCGCCAGGTTTTGGTATGCTTGATGAGTACGGG‑CG‑ACGGCTTTCTGCCCGTCAGAAATTAACCCGCGAGACG‑CTGATATTCTTTGGCAATTT‑CGCTCGCCAGAATGGC‑GTTGTTGAACACCAGCTGGATGTTGGATTTCAGGCTGTCACCGCCGGTCAGTTCAGCAACGCGCGCCAGCAGGAATGGCGTACTTTCTTTACCaa                                                                                                                                                         >  1:699732/1‑239 (MQ=255)

                                                                         aaTGATTTTCCCTT‑CATGCCGGTAAACCCGGCGTCAGCGCCAGGTTTTGGTATGCTTGATGAGTACGGG‑CG‑ACGGCTTTCTGCCCGTCAGAAATTAACCCGCGAGACG‑CTGATATTCTTTGGCAATTT‑CGCTCGCCAGAATGGC‑GTTGTTGAACACCAGCTGGATGTTGGATTTCAGGCTGTCACCGCCGGTCAGTTCAGCAACGCGCGCCAGCAGGAATGGCGTACTTTCTTTACCaa                                                                                                                                                         >  2:978712/1‑239 (MQ=255)

                                                                          aTGATTTTCCCTT‑CATGCCGGTAAACCCGGCGTCAGCGCCAGGTTTTGGTATGCTTGATGAGTACGGG‑CG‑ACGGCTTTCTGCCCGTCAGCAATTCACCCGCGAGACG‑CTGATATTCTTTGGCAATTA‑CGCTCGCCAGAATGGC‑GTTGTTGAACACCAGCTGGATGTTGGATTTCAGGCTGTCACCGCCGGTCAGTTCAGCAACGCGCGCCAGCAGGAATGGCGTACTTTCTTTACCAAt                                                                                                                                                        >  1:762991/1‑239 (MQ=255)

                                                                               tttCCCTT‑CATGCCGGTAAACCCGGCGTCAGCGCCAGGTTTTGGTATGCTTGATGAGTACGGG‑CG‑ACGGCTTTCTGCCCGTCAGAAATTAACCCGCGAGACG‑CTGATATTCTTTGGCAATTT‑CGCTCGCCAGAATGGC‑GTTGTTGAACACCAGCTGGATGTTGGATTTCAGGCTGTCACCGCCGGTCAGTTCAGCAACGCGCGCCAGCAGGAATGGCGTACTTTCTTTACCAATGACt                                                                                                                                                    >  1:244428/1‑238 (MQ=255)

                                                                                 tCCCTT‑CATGCCGGTAACCCCGGCGTCAGCGCCAGGTTTTGGTATGCTTGATGAGTACGGG‑CG‑ACGGCTTTCTGCCCGTCAGAAATTACCCCGCGAGCCG‑CTGATATTCTTTGGCAATTT‑CGCTCGCCAGAATGGC‑GTTGTTGAACACCAGCTGGATGTTGGATTTCAGGCTGTCACCGCCGGTCAGTTCAGCAACGCGCGCCAGCAGGAATGGCGTACTTTCTTTACCAATGACTccc                                                                                                                                                 <  1:1103427/239‑1 (MQ=255)

                                                                                   cccgtaaTGCGGGTAACCCCGGGGTAGGCCCAGGGTTTTGGTAGGCTGGATG‑CAACGGGTCG‑CGGGTGTTCTACCCCTCA‑AAATTTACCCCAGGAACGTCTGATATTTTTGGGCATTTT‑CGCCCGCCAGAATGGG‑TTTGTTGAACACCGGCGGGATTTTGTATTTCAGGCTGTCGCCGCCGGTCAGTTCAGCAACGCGCGCCAGCAGGAATGGCGTACTTTCTTTACCAATGACTCCCt                                                                                                                                                <  2:1021381/233‑1 (MQ=255)

                                                                                                  caCCCGGCGTCAGCGCCAGGTTTTGGTATGCTTGATGAGTACGGG‑CG‑ACGGCTTTCTGCCCGTCAGAAATTAACCCGCGAGACG‑CTGATATTCTTTGGCAATTT‑CGCTCGCCAGAATAGC‑GTTGTTGAACACCAGCTGGATGTTGGATTTCAGGCTGTCACCGCCGGTCAGTTCAGCAACGCGCGCCAGCAGGAATGGCGTACTTTCTTTACCAATGACTCCCTGAgcttcagcttca                                                                                                                                  >  1:505923/2‑238 (MQ=255)

                                                                                                      cGGCGTCAGAGCCAGGTTGTGGTATGCTTGATGAGTACGGG‑CG‑ACGGTTTTCTGCCCGTCAGAAATTAACCCGCGAGACG‑CTGATATTCTTTGGCATTTT‑CGCTCGCCAGAATGGC‑GTTGTTGACCCCCAGCGGGATGTTGGATTTCAGGCTGTCACCGCCGGTCAGTTCAGCAACGCGCGCCAGCAGGAATGGCGTACTTTCTTTACCAATGACTCCCTGAGCTTCAGCTTCAGCTAc                                                                                                                             <  2:53306/239‑1 (MQ=255)

                                                                                                       ggCGTCAGCGCCAGGTTTTGGTATGCTTGATGAGTACGGG‑CG‑ACGGCTTTCTGCCCGTCAGAAATTAACCCGCGAGACG‑CTGATATTCTTTGGCAATTT‑CGCTCGCCAGAATGGC‑GTTGTTGAACACCAGCTGGATGTTGGATTTCAGGCTGTCACCGCCGGTCAGTTCAGCAACGCGCGCCAGCAGGAATGGCGTACTTTCTTTACCAATGACTCCCTGAGCTTCAGCTTCAGCTAcc                                                                                                                            >  2:1087588/1‑239 (MQ=255)

                                                                                                        gCGTCGGCGCCAGGTTTGGGTAGGC‑TGAGGGGGACGGG‑GGTACGGCTTTCTGCCCGTGGTAAATTAACCCGAGAGGCG‑CTGATATTTTTGGGCAATTG‑CGCGCGCCAGAATGGC‑TTGGTTGAACCCCGGCTGGATGTTGGATTCCAGGCTGTCACCGCCGGTCAGTTCAGCAACGCGCGCCAGCAGGAATGGCGTACTTTCTTTACCAATGACTCCCTGAGCTTCAGCTTCAGCTAcc                                                                                                                            <  2:1250935/238‑1 (MQ=255)

                                                                                                         cGTCAGCGCCAGGTTTTGGTATGCTTGATGAGTACGGG‑CG‑ACGGCTTTCTGCCCGTCAGAAATTAACCCGCGAGACG‑CTGATATTCTTTGGCAATTT‑CGCTCGCCAGAATGGC‑GTTGTTGAACACCAGCTGGATGTTGGATTTCAGGCTGTCACCGCCGGTCAGTTCAGCAACGCGCGCCAGCAGGAATGGCGTACTTTCTTTACCAATGACTCCCTGAGCTTCAGCTTCAGCTACCg                                                                                                                           >  2:1040621/1‑238 (MQ=255)

                                                                                                            cAGCGCCCGGTTTTGGTATGCTTGATGAGTACGGG‑GG‑ACGGCTTTCTGCCCGTCAAAAATTAACCCGCGAGACG‑CTGATATTCTTGGGCAATTT‑CGCGCGCAAGAATGGC‑GTTGTTGAACACCAGCGGGATGTTGGATTTCAGCGTGTCACCGCCGGTCAGTTCAGCAACGCGCGCCAGCAGGAATGGCGTACTTTCTTTACCAATGACTCCCTGAGCTTCAGCTTCAGCTACCGCCt                                                                                                                        <  2:942518/238‑1 (MQ=255)

                                                                                                              gcgcCAGGTTTTGGTATGCTTGATGAGTACGGG‑CG‑ACGGCTTTCTGCCCGTCAGAAATTAACCCGCGAGACG‑CTGATATTCTTTGGCAATTT‑CGCTCGCCAGAATGGC‑GTTGTTGAACACCAGCTGGATGTTGGATTTCAGGCTGTCACCGCCGGTCAGTTCAGCAACGCGCGCCAGCAGGAATGGCGTACTTTCTTTACCAATGACTCCCTGAGCTTCAGCTTCAGCTACCGCCTga                                                                                                                      >  1:95957/1‑238 (MQ=255)

                                                                                                               cgcCAGGTTTTGGTATGCTTGATGAGTACGGG‑CG‑ACGGCTTTCTGCCCGTCAGAAATTAACCCGCGAGACG‑CTGATATTCTTTGGCAATTT‑CGCTCGCCAGAATGGC‑GTTGTTGAACACCAGCTGGATGTTGGATTTCAGGCTGTCACCGCCGGTCAGTTCAGCAACGCGCGCCAGCAGGaa                                                                                                                                                                             >  1:1047368/1‑182 (MQ=255)

                                                                                                                   ggtttttGGTATTCTTGATGGGTACGGG‑CG‑ACGGTTTTCTGCCCGCAAAAAATTAACCCGCGGGACG‑CTGATTTTCTTTGGCATTTT‑CCCCCGCCAGAATGGC‑GTTGTTGACCCCCAGCTGGTTGTGGGATTCCGGGCTGTCACCGCCGGTCAGTTCAGCAACGCGCGCCAGCAGGAATGGCGTACTTTCTTTACCAATGACTCCCTGAGCTTCAGCTTCAGCTACCGCCTgatcgatc                                                                                                                <  2:848659/236‑1 (MQ=255)

                                                                                                                    tgTTGTGGTAGGATGGATGAGTAGGGC‑CG‑CCGGCTTTCGGCCCTACAGAAATTACCCCGCGAGCCG‑TTGATTTTCTTTGGAAATTC‑CGCTGGCAAGAAGGGT‑GTTGTTGAACCCCAGCTGGGTGTGGTATTTCAGGCTGTCACCGCCGGTCAGTTCAGCAACGCGCGCCAGCAGGAATGGCGTACTTTCTTTACCAATGACTCCCTGAGCTTCAGCTTCAGCTACCGCCTgatcgatc                                                                                                                <  2:762991/237‑1 (MQ=255)

                                                                                                                      ttttGTTATGCTTGAGGTGTCCGGG‑CG‑ACGTCTTTCTGCCCGCCAAAAATTAACCCGCGAGACG‑TTGTTTTTCTTTGGCATTTT‑CGCTCGCCAGAATGGC‑TTTGTTGAACCCCGGCTGGATGTTGGATTGCAGGCTGTCACCGCCGGTCAGTTCAGCAACGCGCGCCAGCAGGAATGGCGTACTTTCTTTACCAATGACTCCCTGAGCTTCAGCTTCAGCTACCGCCTGATCGATcgc                                                                                                              <  2:800186/238‑1 (MQ=255)

                                                                                                                      ttttGGTATGCTGGATGAGTCCGGG‑CG‑ACGGTTTTCTGCCCGCAAGAAATTAACCCGCGAGACG‑CTGATATTCTTTGGAAATTT‑CGCTCGACAGAATGGC‑TTTGTTGAACACCAGCTGGATGTTGGATTTCAGGCTGTCACCGCCGGTCAGTTCAGCAACGCGCGCCAGCAGGAATGGCGTACTTTCTTTACCAATGACTCCCTGAGCTTCAGCTTCAGCTACCGCCTGATCGATcgc                                                                                                              <  2:640000/238‑1 (MQ=255)

                                                                                                                      tttgGGTATCCTTGAGGGTTCCGGG‑CG‑CCGCCTTTCTCCCCGTCAAAAATTACCCCGCGAGACG‑CTTATATTCTTTGGAATTTT‑CGCTCGCCAGAATGGC‑GTTGTTAAACCCAAGCTGGGTTTTGGTTTTAAGGTTGTCACCGCCGGTCAGTTCAGCAACGCGCGCCAGCAGGAATGGCGTACTTTCTTTACCAATGACTCCCTGAGCTTCAGCTTCAGCTACCGCCTGATCGATcgc                                                                                                              <  2:1183898/238‑1 (MQ=255)

                                                                                                                      ttgtGGTATGCTTGATGAGTACGGG‑CG‑CCGGCTTTCTGCCCGTCAGAAATTAACCCGCGAGACG‑CTGATATTCTTTGGCAATTT‑CGCCCGCCAGAATGGC‑GTTGTGGAACACCAGCTGGATGTTGAATTTCAGGCTGTCACCGCCGGTCAGTTCAGCAACGCGCGCCAGCAGGAATGGCGTACTTTCTTTACCAATGACTCCCTGAGCTTCAGCTTCAGCTACCGCCTgatcgatc                                                                                                                <  1:193725/233‑1 (MQ=255)

                                                                                                                         tGGTGTGCTTGATGAGTCCGGC‑GG‑ACGGCTTTCGGCCCGACAGAAATTACCCCGCGAGACG‑CTGATATTCTTTGGCAATTT‑CGCTCGCCAGAAGGGC‑GTTGTTGAACACCAGTTGGATGTTGGATTTCAGGCTGTCACCGCCGGTCAGTTCAGCAACGCGCGCCAGCAGGAATGGCGTACTTTCTTTACCAATGACTCCCTGAGCTTCAGCTTCAGCTACCGCCTGATCGATCGCCGCa                                                                                                          <  2:450076/239‑1 (MQ=255)

                                                                                                                                cTTGATGAGTACGGG‑CG‑ACGGCTTTCTGCCCGTCAGAAATTAACCCGCGAGACG‑CTGATATTCTTTGGCAATTT‑CGCTCGCCAGAATGGC‑GTTGTTGAACACCAGCTGGATGTTGGATTTCAGGCTGTCACCGCCGGTCAGTTCAGCAACGCGCGCCAGCAGGAATGGCGTACTTTCTTTACCAATGACTCCCTGAGCTTCAGCTTCAGCTACCGCCTGATCGATCGCCGCATTGa                                                                                                      <  1:539062/236‑1 (MQ=255)

                                                                                                                                cTTGATGAGTACGGG‑CG‑ACGGCTTTCTGCCCGTCAGAAATTAACCCGCGAGACG‑CTGATATTCTTTGGCAATTT‑CGCTCGCCAGAATGGC‑GTTGTTGAACACCAGCTGGATGTTGGATTTCAGGCTGTCACCGCCGGTCAGTTCAGCAACGCGCGCCAGCAGGAATGGCGTACTTTCTTTACCAATGACTCCCTGAGCTTCAGCTTCAGCTACCGCCTGATCGATCGCCGCATTGa                                                                                                      >  2:539062/1‑236 (MQ=255)

                                                                                                                                cTTGATGAGTACGGG‑CG‑ACGGCTTTCTGCCCGTCAGAAATTAACCCGCGAGACG‑CTGATATTCTTTGGCAATTT‑CGCTCGCCAGAATGGC‑GTTGTTGAACACCAGCTGGATGTTGGATTTCAGGCTGTCACCGCCGGTCAGTTCAGCAACGCGCGCCAGCAGGAATGGCGTACTTTCTTTACCAATGACTCCCTGAGCTTCAGCTTCAGCTACCGCCTGATCGATCGCCGCATTGATAg                                                                                                   >  1:1339185/1‑239 (MQ=255)

                                                                                                                                cTTGATGAGTACGGG‑CG‑ACGGCTTTCTGCCCGTCAGAAATTAACCCGCGAGACG‑CTGATATTCTTTGGCAATTT‑CGCTCGCCAGAATGGC‑GTTGTTGAACACCAGCTGGATGTTGGATTTCAGGCTGTCACCGCCGGTCAGTTCAGCAACGCGCGCCAGCAGGAATGGCGTACTTTCTTTACCAATGACTCCCTGAGCTTCAGCTTCAGCTACCGCCTGATCGATCGCCGCATTGATAg                                                                                                   >  2:177428/1‑239 (MQ=255)

                                                                                                                                cTTGATGAGTACGGG‑CG‑ACGGCTTTCTGCCCGTCAGAAATTAACCCGCGAGACG‑CTGATATTCTTTGGCAATTT‑CGCTCGCCAGAATGGC‑GTTGTTGAACACCAGCTGGATGTTGGATTTCAGGCTGTCACCGCCGGTCAGTTCAGCAACGCGCGCCAGCAGGAATGGCGTACTTTCTTTACCAATGACTCCCTGAGCTTCAGCTTCAGCTACCGCCTGATCGATCGCCGCATTGATAg                                                                                                   >  1:766404/1‑239 (MQ=255)

                                                                                                                                 ttGATGAGTACGGG‑CG‑ACGGCTTTCTGCCCGTCAGAAATTAACCCGCGAGACG‑CTGATATTCTTTGGCAATTT‑CGCTCGCCAGAATGGC‑GTTGTTGAACACCAGCTGGATGTTGGATTTCAGGCTGTCACCGCCGGTCAGTTCAGCAACGCGCGCCAGCAGGAATGGCGTACTTTCTTTACCAATGACTCCCTGAGCTTCAGCTTCAGCTACCGCCTGATCGATCGCCGCATTGATAgt                                                                                                  <  1:798474/239‑1 (MQ=255)

                                                                                                                                         tACGGG‑CG‑ACGGCTTTCTGCCCGTCAGAAATTAACCCGCGAGACG‑CTGATAATCTTTGGCAATTT‑CGCTCGCCAGAATGGC‑GTTGTTGAACACCAGCTGGATGTTGGATTTCAGGCTGTCACCGCCGGTCAGTTCAGCAACGCGCGCCAGCAGGAATGGCGTACTTTCTTTACCAATGACTCCCTGAGCTTCAGCTTCAGCTACCGCCTGATCGATCGCCGCATTGATAGTGTGTTCCg                                                                                          >  1:32475/1‑239 (MQ=255)

                                                                                                                                           cGGG‑CG‑ACGGCTTTCTGCCCGTCAGAAATTAACCCGCGAGACG‑CTGATATTCTTTGGCAATTT‑CGCTCGCCAGAATGGC‑GTTGTTGAACACCAGCTGGATGTTGGATTTCAGGCTGTCACCGCCGGTCAGTTCAGCAACGCGCGCCAGCAGGAATGGCGTACTTTCTTTACCAATGACTCCCTGAGCTTCAGCTTCAGCTACCGCCTGATCGATCGCCGCATTGATAGTGTGTTCCGGc                                                                                        >  1:232306/1‑239 (MQ=255)

                                                                                                                                           cGGG‑CG‑ACGGCTTTCTGCCCGTCAGAAATTAACCCGCGAGACG‑CTGATATTCTTTGGCAATTT‑CGCTCGCCAGAATGGC‑GTTGTTGAACACCAGCTGGATGTTGGATTTCAGGCTGTCACCGCCGGTCAGTTCAGCAACGCGCGCCAGCAGGAATGGCGTACTTTCTTTACCAATGACTCCCTGAGCTTCAGCTTCAGCTACCGCCTGATCGATCGCCGCATTGATAGTGTGTTCCGGc                                                                                        >  1:781465/1‑239 (MQ=255)

                                                                                                                                             gg‑CG‑ACGGCTTTCTGCCCGTCAGAAATTAACCCGCGAGACG‑CTGATATTCTTTGGCAATTT‑CGCTCGCCAGAATGGC‑GTTGTTGAACACCAGCTGGATGTTGGATTTCAGGCTGTCACCGCCGGTCAGTTCAGCAACGCGCGCCAGCAGGATTGGCGTACTTTCTTTACCAATGACTCCCTGAGCTTCAGCTTCAGCTACCGCCTGATCGATTGCCGCCTTGATa                                                                                                    >  1:1026647/1‑225 (MQ=255)

                                                                                                                                              gggcgtggtgtttCGGCCCGTCAGAAATTCACCCGCGAGACG‑CTGATATTCTTTGGCAATTC‑CGCCCGCCAGAATGGC‑GTTGTTGAACACCAGCTGGATGTTGGATTCCAGGCTGTCACCGCCGGTCAGTTCAGCAACGCGCGCCAGCAGGAATGGCGTACTTTCTTTACCAATGACTCCCTGAGCTTCAGCTTCAGCTACCGCCTGATCGATCGCCGCATTGATAGTGTGTTCCGGc                                                                                        <  2:1267256/228‑1 (MQ=255)

                                                                                                                                                     ggCTTTCTGCCCGTCAGAAATTAACCCGCGAGACG‑CTGATATTCTTTGGCAATTT‑CGCTCGCCAGAATGGC‑GTTGTTGAACACCAGCTGGATGTTGGATTTCAGGCTGTCACCGCCGGTCAGTTCAGCAACGCGCGCCAGCAGGAATGGCGTACTTTCTTTACCAATGACTCCCTGAGCTTCAGCTTCAGCTACCGCCTGatcgatcg                                                                                                               >  1:676581/1‑208 (MQ=255)

                                                                                                                                                     ggCTTTCTGCCCGTCAGAAATTAACCCGCGAGACG‑CTGATATTCTTTGGCAATTT‑CGCTCGCCAGAATGGC‑GTTGTTGAACACCAGCTGGATGTTGGATTTCAGGCTGTCACCGCCGGTCAGTTCAGCAACGCGCGCCAGCAGGAATGGCGTACTTTCTTTACCAATGACTCCCTGAGCTTCAGCTTCAGCTACCGCCTGATCGATCGCCGCATTGATAGTGTGTTCCGGCATAGCaa                                                                                 >  1:71781/1‑238 (MQ=255)

                                                                                                                                                     ggCTTTCTGCCCGTCAGAAATTAACCCGCGAGACG‑CTGATATTCTTTGGCAATTT‑CGCTCGCCAGAATGGC‑GTTGTTGAACACCAGCTGGATGTTGGATTTCAGGCTGTCACCGCCGGTCAGTTCAGCAACGCGCGCCAGCAGGAATGGCGTACTTTCTTTACCAATGACTCCCTGAGCTTCAGCTTCAGCTACCGCCTGATCGATCGCCGCATTGATAGTGTGTTCCGGCATAGCaa                                                                                 >  1:2581/1‑238 (MQ=255)

                                                                                                                                                      gttctctcCCCGTCAGAAATTACCCCGAGGCAGG‑CAGTATTTCTTGGAAAATTC‑CGCTCGCGAGAGGGGC‑GTTGTTGAACACCAGCTGGGTGTTGGATTTCTGGCTGTCTCCGCCGGTCAGTTCAGCAACGCGCGCCAGCAGGAATGGCGTACTTTCTTTACCAATGACTCCCTGAGCTTCAGCTTCAGCTACCGCCTGATCGATCGCCGCATTGATAGTGTGTTCCGGCATAGCaaa                                                                                <  2:785537/234‑1 (MQ=255)

                                                                                                                                                                 gTCAGAAATTAACCCGCGAGACG‑CTGATATTCTTTGGCAATTT‑CGCTCGCCAGAATGGC‑GTTGTTGAACACCAGCTGGATGTTGGATTTCAGGCTGTCACCGCCGGTCAGTTCAGCAACGCGCGCCAGCAGGAATGGCGTACTTTCTTTACCAATGACTCCCTGAGCTTCAGCTTCAGCTACCGCCTGATCGATCGCCGCATTGATAGTGTGTTCCGGCATAGCAAACTGTTCCGGGa                                                                     >  1:1000389/1‑238 (MQ=255)

                                                                                                                                                                 gTCAGAAATAACCCCGCGGGACC‑CTGATATTTGTTGGCAATTG‑CGCTCGCCAGAAGGGC‑GTTGTTGAACCCCAGCGGGTTGTTGGATTTCAGGCTGCCACCGCCGGTCAGTTCAGCAACGCGCGCCAGCAGGAATGGCGTACTTTCTTTACCAATGACTCCCTGAGCTTCAGCTTCAGCTACCGCCTGATCGATCGCCGCATTGATAGTGTGTTCCGGCATAGCAAACTGTTCCGGGAt                                                                    <  2:1100122/239‑1 (MQ=255)

                                                                                                                                                                  tCAGAAATTAACCCGCGAGACG‑CTGATATTCTTTGGCAATTT‑CGCTCGCCAGAATGGC‑GTTGTTGACCACCAGCTGGATGTTGGATTCCAGGCTGTCACCGCCGGTCAGTTCAGCAACGCGCGCCACCAGGAATGGCGTACTTTCTTTACCAATGACTACCTGGGCTTAAGCTTCAGCTACGGCTTACTCGATCACCGCTTTGATAGTCAGTTCCGTCATAACAACCTGTTCCGCGATc                                                                   >  2:759721/1‑239 (MQ=255)

                                                                                                                                                                         ttAACCCGCGAGACG‑CTGATATTCTTTGGCAATTT‑CGCTCGCCAGAATGGC‑GTTGTTGAACACCAGCGGGATGTTGGATTTCAGGCTGTCACCGCCGGTCAGTTCAGCAACGCGCGCCAGCAGGAATGGCGTACTTTCTTTACCAATGACTCCCTGAGCTTCAGCTTCAGCTACCGCCTGATCGATCGCCGCATTGATAGTGTGTTCCGGCATAGCAAACTGTTCCGGGATCGGGTTCg                                                            <  1:823336/239‑1 (MQ=255)

                                                                                                                                                                                gcgAGACG‑GTGATATTCTTGGGCAATTT‑CGCTCGCCAGAATGGC‑TTTGTTAAACCCCGGCGGGTTGTTGGATTTCAGGCTGTCACCGCCGGTCAGTTCAGCAACGCGCGCCAGCAGGAATGGCGTACTTTCTTTACCAATGACTCCCTGAGCTTCAGCTTCAGCTACCGCCTGATCGATCGCCGCATTGATAGTGTGTTCCGGCATAGCAAACTGTTCCGGGATCGGGTTCGCTACCAc                                                     <  2:336501/239‑1 (MQ=255)

                                                                                                                                                                                gcgAGACG‑CTGATATTCTTTGGCAATTT‑CGCTCGCCAGAATGGC‑GTTGTTGAACACCAGCTGGATGTTGGATTTCAGGCTGTCACCGCCGGTCAGTTCAGCAACGCGCGCCAGCAGGAATGGCGTACTTTCTTTACCAATGACTCCCTGAGCTTCAGCTTCAGCTACCGCCTGATCGATCGCCGCATTGCTAGTGTGTTCCGGCATAGCAAACTGTTCCGGGATCGGGTTCGCTACCAc                                                     >  2:1258210/1‑239 (MQ=255)

                                                                                                                                                                                gcgAGACG‑CTGATATTCTTTGGCAATTT‑CGCTCGCCAGAATGGC‑GTTGTTGAACACCAGCTGGATGTTGGATTTCAGGCTGTCACCGCCGGTCAGTTCAGCAACGCGCGCCAGCAGGAATGGCGTACTTTCTTTACCAATGACTCCCTGAGCTTCAGCTTCAGCTACCGCCTGATCGATCGCCGCATTGATAGTGTGTTCCGGCATAGCAAACTGTTCCGGGATCGGGTTCGCTACCAc                                                     >  2:311051/1‑239 (MQ=255)

                                                                                                                                                                                gcgAGACG‑CTGATATTCTTTGGCAATTT‑CGCTCGCCAGAATGGC‑GTTGTTGAACACCAGCTGGATGTTGGATTTCAGGCTGTCACCGCCGGTCAGTTCAGCAACGCGCGCCAGCAGGAATGGAGTAATTTCTTTACCAATGACTCCCTGAGCTTCAGCTTCAGCTACCGCCTGATCGATCGCCGCATTGATAGTGTGTTCCGGCATAGCAAACTGCTACGGGATCGGGGTCGCTAGCAc                                                     >  1:696648/1‑239 (MQ=255)

                                                                                                                                                                                   ggccG‑CTGTTTTTCTTTGGCAATTT‑CGCCGCCCAGAATGGG‑TTTGTTGAACACCAGCTGGGTGTTGGTTTTCAGGCTGTCACCGCCGGTCAGTTCAGCAACGCGCGCCAGCAGGAATGGCGTACTTTCTTTACCAATGACTCCCTGAGCTTCAGCTTCAGCTACCGCCTGATCGATCGCCGCATTGATAGTGTGTTCCGGCATAGCAAACTGTTCCGGGATCGGGTTCGCTACCACGa                                                   <  2:593463/235‑1 (MQ=255)

                                                                                                                                                                                   ggagcgttaatatTTTTTGGCATTTT‑CCCTCGCC‑GGATGGCGGTGGTTAAACACAAGCTGGATTTTGGATTTCAGGTTGCCACCGCCGGTCAGTTCAGCAACGCGCGCCAGCAGGAATGGCGTACTTTCTTTACCAATGACTCCCTGAGCTTCAGCTTCAGCTACCGCCTGATCGATCGCCGCATTGATAGTGTGTTCCGGCATAGCAAACTGTTCCGGGATCGGGTTCGCTACCACGa                                                   <  2:455857/230‑1 (MQ=255)

                                                                                                                                                                                   agtcG‑CTGATATTTTTGGGCAATTC‑CGACCGCCGAAAGGGC‑GTTGTAGACCCCCAGCTGGATGTTGGATTTCGGGCTGCCACCGCCGGTCAGTTCAGCAACGCGCGCCAGCAGGAATGGCGTACTTTCTTTACCAATGACTCCCTGAGCTTCAGCTTCAGCTACCGCCTGATCGATCGCCGCATTGATAGTGTGTTCCGGCATAGCAAACTGTTCCGGGATCGGGTTCGCTACCACGa                                                   <  2:386122/235‑1 (MQ=255)

                                                                                                                                                                                     agccgcgtctattttaggCGATTTCCGCTCGCCAGAAGGCC‑GTTTTTGACCCCCAGCGGGTTGTTGATTTCCGGGCTCTCACCGCCGGTCAGTTCAGCAACGCGCGCCAGCAGGAATGGCGTACTTTCTTTACCAATGACTCCCTGAGCTTCAGCTTCAGCTACCGCCTGATCGATCGCCGCATTGATAGTGTGTTCCGGCATAGCAAACTGTTCCGGGATCGGGTTCGCTACCACGa                                                   <  2:708879/222‑1 (MQ=255)

                                                                                                                                                                                      cG‑CTGTTTTTTTTGGGCATTCT‑CGCCCCCAAGAATGGC‑GTTGTTGACCCCCAGCTGTTTGTTGGATTTAAGGCTGTCGCCGCCGGTCAGTTCAGCAACGCGCGCCAGCAGGAATGGCGTACTTTCTTTACCAATGACTCCCTGAGCTTCAGCTTCAGCTACCGCCTGATCGATCGCCGCATTGATAGTGTGTTCCGGCATAGCAAACTGTTCCGGGATCGGGTTCGCTACCACGAGGcc                                               <  2:699732/239‑1 (MQ=255)

                                                                                                                                                                                      cG‑CTGATATTCTTTGGAAATTT‑CGCTCGCCAGAATGGC‑GTTGTTGAACACCAGCGGGATGTTGGATTTCAGGCTGTCACCGCCGGTCAGTTCAGCAACGCGCGCCAGCAGGAATGGCGTACTTTCTTTACCAATGACTCCCTGAGCTTCAGCTTCAGCTACCGCCTGATCGATCGCCGCATTGATAGTGTGTTCCGGCATAGCAAACTGTTCCGGGATCGGGTTCGCTACCACGAGGc                                                <  1:1257639/238‑1 (MQ=255)

                                                                                                                                                                                                 cTTTGGCAATTT‑CGCTCGCCAGAATGGC‑GTTGTTGAACACCAGCTGGATGTTGGATTTCAGGCTGTCACCGCCGGTCAGTTCAGCAACGCGCGCCAGCAGGAATGGCGTACTTTCTTTACCAATGACTCCCTGAGCTTCAGCTTCAGCTACCGCCTGATCGATCGCCGCATTGATAGTGTGTTCCGGCATAGCAAACTGTTCCGGGATCGGGTTCGCTACCACGAGGCCACCGTTCAg                                      >  1:701964/1‑238 (MQ=255)

                                                                                                                                                                                                  tttGGCAATTT‑CGCTCGCCAGAATGGC‑GTTGTTGAACACCAGCTGGATGTTGGATTTCAGGCTGTCACCGCCGGTCAGTTCAGCAACGCGCGCCAGCAGGAATGGCGTACTTTCTTTACCAATGACTCCCTGAGCTTCAGCTTCAGCTACCGCCTGATCGATCGCCGCATTGATAGTGTGTTCCGGCATAGCAAACTGTTCCGGGATCGGGTTCGCTACCACGAGGCCACCGTTCAGa                                     >  1:935215/1‑238 (MQ=255)

                                                                                                                                                                                                  ttgggaaaTGT‑CGCCCGCCAGAAGGGC‑GTTGTTGAACACCAGCGGGATGTTGGATTTCAGGGTGTCACCGCCGGTCAGTTCAGCAACGCGCGCCAGCAGGAATGGCGTACTTTCTTTACCAATGACTCCCTGAGCTTCAGCTTCAGCTACCGCCTGATCGATCGCCGCATTGATAGTGTGTTCCGGCATAGCAAACTGTTCCGGGATCGGGTTCGCTACCACGAGGCCACCGTTCAGAc                                    <  2:1348551/233‑1 (MQ=255)

                                                                                                                                                                                                    tGGCAATTT‑CGCTCGCCAGAATGGC‑GTTGTTGAACACCAGCTGGATGTTGGATTTCAGGCTGTCACCGCCGGTCAGTTCAGCAACGCGCGCCAGCAGGAATGGCGTACTTTCTTTACCAATGACTCCCTGAGCTTCAGCTTCAGCTACCGCCTGATCGATCGCCGCATTGATAGTGTGTTCCGGCATAGCAAACTGTTCCGGGATCGGGTTCGCTACCACGAGGCCACCGTTCAGAcc                                   >  2:949938/1‑238 (MQ=255)

                                                                                                                                                                                                      gCAATTT‑CGCTCGCCAGAATGGC‑GTTGTTGAACACCAGCTGGATGTTGGATTTCAGGCTGTCACCGCCGGTCAGTTCAGCAACGCGCGCCAGCAGGAATGGCGTACTTTCTTTACCAATGACTCCCTGAGCTTCAGCTTCAGCTACCGCCTGATCGATCGCCGCATTGATAGTGTGTTCCGGCATAGCAAACTGTTCCGGGATCGGGTTCGCTACCACGAGGCCACCGTTCAGACCGCt                                >  1:686520/1‑239 (MQ=255)

                                                                                                                                                                                                      gCAATTT‑CGCTCGCCAGAATGGC‑GTTGTTGAACACCAGCTGGATGTTGGATTTCAGGCTGTCACCGCCGGTCAGTTCAGCAACGCGCGCCAGCAGGAATGGCGTACTTTCTTTACCAATGACTCCCTGAGCTTCAGCTTCAGCTACCGCCTGATCGATCGCCGCATTGATAGTGTGTTCCGGCATAGCAAACTGTTCCGGGATCGGGTTCGCTACCACGAGGCCACCGTTCAGACCGCt                                >  1:152805/1‑239 (MQ=255)

                                                                                                                                                                                                          ttt‑CGCTCGCCAGAATGGC‑GTTGTTGAACACCAGCTGGATGTTGGATTTCAGGCTGTCACCGCCGGTCAGTTCAGCAACGCGCGCCAGCAGGAATGGCGTACTTTCTTTACCAATGACTCCCTGAGCTTCAGCTTCAGCTACCGCCTGATCGATCGCCGCATTGATAGTGTGTTCCGGCATAGCAAACTGTTCCGGGATCGGGTTCGCTACCACGAGGCCACCGTTCAGACCGCTTTGc                            <  1:684111/239‑1 (MQ=255)

                                                                                                                                                                                                           tt‑CGCGCGCAAGAATGGG‑GTTGTTGAACCCCGGCTGGTTGTGGGTTTTCGGGCTGTCTCCGCCGGTCAGTTCAGCAACGCGCGCCAGCAGGAATGGCGTACTTTCTTTACCAATGACTCCCTGAGCTTCAGCTTCAGCTACCGCCTGATCGATCGCCGCATTGATAGTGTGTTCCGGCATAGCAAACTGTTCCGGGATCGGGTTCGCTACCACGAGGCCACCGTTCAGACCGCTTTGcc                           <  2:232306/239‑1 (MQ=255)

                                                                                                                                                                                                                   gcCAGAATGGC‑GTTGTTGAACACCAGCTGGATGTTGGATTTCAGGCTGTCACCGCCGGTCAGTTCAGCAACGCGCGCCAGCAGGAATGGCGTACTTTCTTTACCAATGACTCCCTGAGCTTCAGCTTCAGCTACCGCCTGATCGATCGCCGCATTGATAGTGTGTTCCGGCATAGCAAACTGTTCCGGGATCGGGTTCGCTACCACGAGGCCACCGTTCAGACCGCTTTGCCATTTcac                    <  1:414240/239‑1 (MQ=255)

                                                                                                                                                                                                                   gcCAGAATGGC‑GTTGTGGAACCCCGGCTGGGTGTGGGTTTGCGGGCTGTCACCGCCGGTCAGTTCAGCAACGCGCGCCAGCAGGAATGGCGTACTTTCTTTACCAATGACTCCCTGAGCTTCAGCTTCAGCTACCGCCTGATCGATCGCCGCATTGATAGTGTGTTCCGGCATAGCAAACTGTTCCGGGATCGGGTTCGCTACCACGAGGCCACCGTTCAGACCGCTTTGCCATTTcac                    <  2:568376/239‑1 (MQ=255)

                                                                                                                                                                                                                     ttaaaagagacgggttaAACCCCAGCGGGATGTGGGATTCCGGGCTGTCACCGCCGGTCAGTTCAGCAACGCGCGCCAGCAGGAATGGCGTACTTTCTTTACCAATGACTCCCTGAGCTTCAGCTTCAGCTACCGCCTGATCGATCGCCGCATTGATAGTGTGTTCCGGCATAGCAAACTGTTCCGGGATCGGGTTCGCTACCACGAGGCCACCGTTCAGACCGCTTTGCCATTTcac                    <  2:136527/225‑1 (MQ=255)

                                                                                                                                                                                                                        aaTGGC‑GTTGTTGAACACCAGCTGGATGTTGGATTTCAGGCTGTCACCGCCGGTCAGTTCAGCACCGCGCGCCAGCAGGAATGGCGTACTTTCTTTACCAATGACTCCCTGAGCTTCAGCTTCAGCTACCGCCTGATCGATCGCCGCATTGATAGTGTGTTCCGGCATAGCAAACTGTTCCGGGATCGGGTTCGCTACCACGAGGCCACCGTTCAGACCGCTTTGCCATTTCAccacca               >  1:192148/1‑239 (MQ=255)

                                                                                                                                                                                                                         agggC‑GTTGTTGAACCCCAGCGGGATGTTGGATTTCAGGCTGTCACCGCCGGTCAGTTCAGCAACGCGCGCCAGCAGGAATGGCGTACTTTCTTTACCAATGACTCCCTGAGCTTCAGCTTCAGCTACCGCCTGATCGATCGCCGCATTGATAGTGTGTTCCGGCATAGCAAACTGTTCCGGGATCGGGTTCGCTACCACGAGGCCACCGTTCAGACCGCTTTGCCATTTCaccacc                <  2:766404/235‑1 (MQ=255)

                                                                                                                                                                                                                             c‑GTTGTTGAACACCAGCTGGATGTTGGATTTCAGGCTGTCACCGCCGGTCAGTTCAGCAACGCGCGCCAGCAGGAATGGCGTACTTTCTTTACCAATGACTCCCTGAGCTTCAGCTTCAGCTACCGCCTGATCGATCGCCGCATTGATAGTGTGTTCCGGCATAGCAAACTGTTCCGGGATCGGGTTCGCTACCACGAGGCCACCGTTCAGACCGCTTTGCCATTTCACCACCATTGc           >  2:310081/1‑238 (MQ=255)

                                                                                                                                                                                                                             c‑GTTGTTGAACACCAGCTGGATGTTGGATTTCAGGCTGTCACCGCCGGTCAGTTCAGCAACGCGCGCCAGCAGGAATGGCGTACTTTCTTTACCAATGACTCCCTGAGCTTCAGCTTCAGCTACCGCCTGATCGATCGCCGCATTGATAGTGTGTTCCGGCATAGCAAACTGTTCCGGGATCGGGTTCGCTACCACGAGGCCACCGTTCAGACCGCTTTGCCATTTCACCACCATTGc           <  1:1040621/238‑1 (MQ=255)

                                                                                                                                                                                                                               gtttttGAACCCCGTCTGGTGGTGGGTTTTCAGGCTGCAACCGCCGGTCAGTTCAGCAACGCGCGCCAGCAGGAATGGCGTACTTTCTTTACCAATGACTCCCTGAGCTTCAGCTTCAGCTACCGCCTGATCGATCGCCGCATTGATAGTGTGTTCCGGCATAGCAAACTGTTCCGGGATCGGGTTCGCTACCACGAGGCCACCGTTCAGACCGCTTTGCCATTTCACCACCATTGCa          <  2:244428/238‑1 (MQ=255)

                                                                                                                                                                                                                                ttgttgAACACCAGCTGGATGTTGGATTTCAGGCTGTCACCGCCGGTCAGTTCAGCAACGCGCGCCAGCAGGAATGGCGTACTTTCTTTACCAATGACTCCCTGAGCTTCAGCTTCAGCTACCGCCTGATCGATCGCCGCATTGATAGTGTGTTCCGGCATAGCAAACTGTTCCGGGATCGGGTTCGCTACCACGAGGCCACCGTTCAGACCGCTTTGCCATTTCACCACCATTGCACg        >  1:1341083/1‑239 (MQ=255)

                                                                                                                                                                                                                                   ttgAACACCAGCTGGATGTTGGATTTCAGGCTGTCACCGCCGGTCAGTTCAGCAACGCGCGCCAGCAGGAATGGCGTACTTTCTTTACCAATGACTCCCTGAGCTTCAGCTTCAGCTACCGCCTGATCGATCGCCGCATTGATAGTGTGTTCCGGCATAGCAAACTGTTCCGGGATCGGGTTCGCTACCACGAGGCCACCGTTCAGACCGCTTTGCCATTTCACCACCATTGCACGGGc     >  2:497189/1‑239 (MQ=255)

                                                                                                                                                                                                                                   ttgAACACCAGCTGGATGTTGGATTTCAGGCTGTCACCGCCGGTCAGTTCAGCAACGCGCGCCAGCAGGAATGGCGTACTTTCTTTACCAATGACTCCCTGAGCTTCAGCTTCAGCTACCGCCTGATCGATCGCCGCATTGATAGTGTGTTCCGGCATAGCAAACTGTTCCGGGATCGGGTTCGCTACCACGAGGCCACCGTTCAGACCGCTTTGCCATTTCACCACCATTGCACGGGc     >  1:642023/1‑239 (MQ=255)

                                                                                                                                                                                                                                     gAACACCAGCTGGATGTTGGATTTCAGGCTGTCACCGCCGGTCAGTTCAGCAACGCGCGCCAGCAGGAATGGCGTACTTTCTTTACCAATGACTCCCTGAGCTTCAGCTTCAGCTACCGCCTGATCGATCGCCGCATTGATAGTGTGTTCCGGCATAGCAAACTGTTCCGGGATCGGGTTCGCTACCACGAGGCCACCGTTCAGACCGCTTTGCCATTTCACCACCATTGCACGGGCaa   <  1:983395/239‑1 (MQ=255)

                                                                                                                                                                                                                                       acacAAGCTGGATGTTGGATTTCAGGCTGTCACCGCCGGTCAGTTCAGCAACGCGCGCCAGCAGGAATGGCGTACTTTCTTTACCAATGACTCCCTGAGCTTCAGCTTCAGCTACCGCCTGATCGATCGCCGCATTGATAGTGTGTTCCGGCATAGCAAACTGTTCCGGGATCGGGTTCGCTACCACGAGGCCACCGTTCAGACCGCTTTGCCATTTCACCACCATTGCACGGGCAAt  <  1:1289781/238‑1 (MQ=255)

TTTTATTCACTGACAACAGAAATGCTATTGCCAGTAACACCACCATCCCCACAACACTTCTCATTATATCCATAATGATTTTCCCTT‑CATGCCGGTAAACCCGGCGTCAGCGCCAGGTTTTGGTATGCTTGATGAGTACGGG‑CG‑ACGGCTTTCTGCCCGTCAGAAATTAACCCGCGAGACG‑CTGATATTCTTTGGCAATTT‑CGCTCGCCAGAATGGC‑GTTGTTGAACACCAGCTGGATGTTGGATTTCAGGCTGTCACCGCCGGTCAGTTCAGCAACGCGCGCCAGCAGGAATGGCGTACTTTCTTTACCAATGACTCCCTGAGCTTCAGCTTCAGCTACCGCCTGATCGATCGCCGCATTGATAGTGTGTTCCGGCATAGCAAACTGTTCCGGGATCGGGTTCGCTACCACGAGGCCACCGTTCAGACCGCTTTGCCATTTCACCACCATTGCACGGGCAAT  >  NC_000913/2257261‑2257723

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`