BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,760,383	A→C	25.9%	V289G (GTG→GGG)	selA ←	selenocysteine synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,760,383	0	A	C	25.9%	187.9 / 29.1	122	V289G (GTG→GGG)	selA	selenocysteine synthase
Reads supporting (aligned to +/- strand): ref base A (54/34); new base C (3/30); total (57/65)
Fisher's exact test for biased strand distribution p-value = 1.20e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 123 total aligned reads displayed.

GCCTGGATTTGAATGACCTCTGCGCTGCGGGTAAGCAGGCGCAGGGTCGGTAATTTTTCACTCAGAGCTTCAGGGTGT‑AAATAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGG‑TCATTTTATCCGCGCGTAATG‑CACGCTTCAGCGGGTGG‑CTTTGCAGGCGGGCGATCATCTCTTTTTTACCAACAATAATTCCTG‑CCTGCGGCCCGCCTAA‑CAA‑CTT‑GTCGCC‑GGAGAAACTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTACTTTCATCAACAGTGCGGTATTTTCATTCACCGCCTGACGAT  >  NC_000913/3760146‑3760617

gCCTGGATTTGAATGACCTCTGCGCTGCGGGTAAGCAGGCGCAGGGTCGGTAATTTTTCACTCAGAGCTTCAGGGTGT‑AAATAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGG‑TCATTTTATCCGCGCGTAATG‑CACGCTTCAGCGGGTGG‑CTTTGCAGGCGGGCGATCATCTCTTTTTTACCAACAATAATTTCTT‑CCTGCGGCCCGCCTAA‑CAA‑CTT‑GTCGCC‑GGAGAAAATCa                                                                                                                                                                                                                                            >  2:782402/1‑238 (MQ=255)

      aTTTGAATGACCTCTGCGCTGCGGGTAAGCAGGCGCAGGGTCGGTAATTTTTCACTCAGAGCTTCAGGGTGT‑AAATAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGG‑TCATTTTATCCGCGCGTAATG‑CACGCTTCAGCGGGTGG‑CTTTGCAGGCGGGCGATCATCTCTTTTTTACCAACAATAATTTCTG‑CCTGCGGCCCGCCTAA‑CAA‑CTT‑GTCGCC‑GGAGAAAACCACCAGAc                                                                                                                                                                                                                                      >  2:207917/1‑238 (MQ=255)

           aaTGACCTCTGCGCTGCGGGTAAGCAGGCGCAGGGTCGGTAATTTTTCACTCAGAGCTTCAGGGTGT‑AAATAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGG‑TCATTTTATCCGCGCGTAATG‑CACGCTTCAGCGGGTGG‑CTTTGCAGGCGGGCGATCATCTCTTTTTTACCAACAATAATTCCTG‑CCTGCGGCCCGCCTAA‑CAA‑CTT‑GTCGCC‑GGAGAAACTCACCAGACTGAcg                                                                                                                                                                                                                                 >  1:748248/1‑238 (MQ=255)

               aCCTCTGCGCTGCGGGTAAGCAGGCGCAGGGTCGGTAATTTTTCACTCAGAGCTTCAGGGTGT‑AAATAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGG‑TCATTTTATCCGCGCGTAATG‑CACGCTTCAGCGGGTGG‑CTTTGCAGGCGGGCGGTCATCTCTTTTTTACCAACAATAATTCCTG‑CCCGCGGCCCCCCTAA‑CAA‑CCT‑GTCGCC‑GGAGAAACTCACCAGCCTGAcccccg                                                                                                                                                                                                                             >  1:241154/1‑238 (MQ=255)

                 ctcgGCGCTGGGGGTAAGCGGGCGCGGGGCCGGTAATTTTTCCCCCAGACCTTAGGGGTGT‑AAATAAAGCCGCACCGTGGTTTCCAGCGCCGCGAGGG‑TCTTTTTACCCGCGCGTAATG‑CACGCTTCAGCGGGTGG‑CTTTGCAGGCGGGCGATCATCTCTTTTTTCCCAACAATAATCCCTG‑CCTGCGGCCCGCCTAA‑CAA‑CTT‑GTCGCC‑GGAAAAACTCACCAGACTGACGCccgccg                                                                                                                                                                                                                          <  2:747172/239‑1 (MQ=255)

                     gcgctgcgGGTAAGCAGGCGCAGGGTCGGTAATTTTTCACTCAGAGCTTCAGGGTGT‑AAATAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGG‑TCATTTTATCCGCGCGTAATG‑CACGCTTCAGCGGGTGG‑CTTTGCAGGCGGGCGATCATCTCTTTTTTACCAACAATAATTCCTG‑CCTGCGGCCCGCCTAA‑CAA‑CTT‑GTCGCC‑GGAGAAACTCACCAGACTGACGCCCGCCGCaa                                                                                                                                                                                                                       >  1:227702/1‑238 (MQ=255)

                      cgctgcgGGTAAGCAGGCGCAGGGTCGGTAATTTTTCACTCAGAGCTTCAGGGTGT‑AAATAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGG‑TCATTTTATCCGCGCGTAATG‑CACGCTTCAGCGGGTGG‑CTTTGCAGGCGGGGCGTCATCTCTTTTTTACCAACAATAATTCCTG‑CCTGCGGCCCGCCCAA‑CCA‑CTT‑GTCGCC‑GGAGAAACTCACCAGAATGACCCCCGCCCCAATc                                                                                                                                                                                                                     >  2:1314062/1‑239 (MQ=255)

                         ggggggTAAGCAGGCGTGGGGGTGTTAATTTTTCACGAAGAGCTGCAGGGTAT‑AAATAAAGACGCAGCGTGTCTTCCAGCGCCGCGAGGG‑TCATTTTACCCGCGCGTAATG‑CACGCTTCAGCGGGTGG‑CTTTGCAGGCGGGCGCTCATCTCTTTTTTACCAACAATAATCCCTG‑CCTGCGCCCCCCCTAA‑CAT‑CTT‑GTCGCC‑GGAGAAACCCACCAGCCTGACGCCCGCCGCAATCaa                                                                                                                                                                                                                   <  2:796582/235‑1 (MQ=255)

                           cgGGTAAGCAGGCGCAGGGTCGGTAATTTTTCACTCAGAGCTTCAGGGTGT‑AAATAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGG‑TCATTTTATCCGCGCGTAATG‑CACGCTTCAGCGGGTGG‑CTTTGCAGGCGGGCGATCATCTCTTTTTTACCAACAATAATTCCTG‑CCTGCGGCCCGCCTAA‑CAA‑CTT‑GTCGCC‑GGAGAAAATCACCAGACTGACGCCCGCCGCCATCAACt                                                                                                                                                                                                                 >  1:498153/1‑238 (MQ=255)

                              gTAAGCAGGCGCAGGGTCGGTAATTTTTCACTCAGAGCTTCAGGGTGT‑AAATAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGG‑TCATTTTATCCGCGCGTAATG‑CACGCTTCAGCGGGTGG‑CTTTGCAGGCGGGCGATCATCTCTTTTTTACCAACAATAATTCCTG‑CCTGCGGCCCGCCTAA‑CAA‑CTT‑GTCGCC‑GGAGAAACTCACCAGACTGACGCCCGCCGCAATCAACTCCt                                                                                                                                                                                                              >  1:585189/1‑238 (MQ=255)

                                                gttagttttcCGCTCAGAGCTTCGGGGTGT‑AAATAAAGACGCAACGGTGCCTCCAGCGCCGGGGGGG‑TATTTTTACCGGCGCGTAATG‑CACGCTTGAGCGGGTGG‑CTTTGCAGGCGGGCGATCATCTTTTTTGTCCCAACAAAAATCCCTC‑CCTGCGCCCCGCCAAA‑CAC‑CTG‑GTCGCC‑GGAGAAACGCCCCAGACTGCCGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCg                                                                                                                                                                                            <  2:14641/233‑1 (MQ=255)

                                                 gTAATTTTTCACTCAGAGCTTCAGGGTGT‑AAATAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGG‑TCATTTTATCCGCGCGTAATG‑CACGCTTCAGCGGGTGT‑CTTTGCAGGCGGGCCCTCCTCCTTTTTTTCCCAACAAAAATTCCTG‑CCTTCGGCCCGCCTAA‑CAA‑ATT‑GTCGCC‑GGCGAAAATCCACAGCATGACTCCCGACCCACACAACTCCCTCGGCATCGGCCATTTctct                                                                                                                                                                                          >  2:224037/1‑236 (MQ=255)

                                                 gTAATTTTTCACTCAGAGCTTCAGGGTGT‑AAATAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGG‑TCATTTTATCCGCGCGTAATG‑CACGCTTCAGCGGGTGG‑CTTTGCAGGCGGGCGGTCATCTCTTTTTTTCCAACAATAATTCCTG‑CCTGCGGCCCGCCTAA‑AAA‑CTT‑TTCGCC‑CGAGAAAATCACCAGACCGACGCCCGCCCCCATCAACTCCTCTGGCCTTTGCCCTTTTgg                                                                                                                                                                                           >  2:705759/1‑238 (MQ=255)

                                                  tAATTTTTCACCCGGAGCTTCAGGGTGA‑AAATAAGGACGCAACGTGGCTCCCAGCGCCGCGAGGG‑TCATTTTATCCGCGCGTAATG‑CACGCTTCAGCGGGTGG‑CTTTGCGGGCGGGCGATCATCTTTTTTTTACCAACAATAATTCCTG‑CCTGCGGCCCGCCTAA‑CAA‑CTT‑GTCGCC‑GGAGAAACTCCCCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCa                                                                                                                                                                                         <  1:460339/239‑1 (MQ=255)

                                                      ttttCACTCAGAGCTTCAGGGTGT‑AAATAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGG‑TCATTTTATCCGCGCGTAATG‑CACGCTTCAGCGGGTGG‑CTTTGCAGGCGGGCGGTCATCTCTTTTTTACCAACAATAATTCCTG‑CCTGCGGCCCGCCTAA‑CAA‑CTT‑GTTGCC‑GGAGAAACTCACCAGACTGACGCCCCCCGCAATCAACTCCTGCGGCATTGGCCCTTTCGGCCAAc                                                                                                                                                                                      >  2:239088/1‑238 (MQ=255)

                                                        ttCACTAATAGCTTCAGGGTGT‑AAATAAAGAGGCAACGTGGCTTCCAGCGCCGCGAGGG‑TGATTTTATCCGCGCGTAATG‑CACGCTTCAGCGGGTGG‑CTTTGCAGGCGGGCGATCAGCTCTTTTTTATCAACAATAATTCCTT‑CCGGCTGCCCGCCTTA‑CAA‑CTT‑TTTGCA‑CGCGCAACTCACCAGCCTGACGCCCGCCGGAATCAAATCCTTCGGCATTGGCTTTTTCGGCCAAcaca                                                                                                                                                                                   >  2:145320/1‑236 (MQ=255)

                                                         tCACTCAGACCTTCAGGGTTT‑AAATAAAGACGCAAGGGGGCTTCCAGCGCCGCGAGGG‑TCATTTTATCCGCGCGTAATC‑CACGCTTCAGCGGGTGG‑CTTTGCGGGCGGGCGATCATCTTTTTTTTCCCAACAATAATTCCTG‑CCTGCGGCCCGCCTAA‑CAA‑CTT‑GTCGCC‑GGAGAACCTCCCCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTa                                                                                                                                                                                  <  1:857656/239‑1 (MQ=255)

                                                         ccccTCAGAGCTTCAGGGTGT‑AAATAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGG‑TCATTTTATCCGCGCGTAATG‑CCCGCTTCAGCGGGTGG‑CTTTGCAGGCGGGCGATCATCTTTTTTTTACCAACAATAATTCCTG‑CCTGCGGCCCGCCTAA‑CAA‑CTT‑GTCGCC‑GGAGAAACTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTa                                                                                                                                                                                  <  1:782402/236‑1 (MQ=255)

                                                         ccACTCAGAGCTTAAGGGTTT‑AAATAAAGACGCAACGTGGCTTCCAGCGCCGCGGGGG‑TCATTTTACCCGCGCGTAATG‑CACGCTTCAGCGGGTGG‑CTTTGCAGGCGGGCGATCATCTCTTTTTTACCAACAATAATTCCTG‑CCTGCGGCCCGCCTAA‑CAA‑CTT‑GTCGCC‑GGAGAAACTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTa                                                                                                                                                                                  <  1:884834/238‑1 (MQ=255)

                                                         ccACGCAGAGCTCCAGGGTGA‑AAAAAAAGGCGCAACGGGGCTCCCCGCGGCGGGGGTG‑TCATTTTACCCGCGCGTAGTG‑CACGCTCCAGGGGGGGG‑TTTGGCAGGCGGGCGATCATTTCTTTTTTCCAAACAAAAATCCCTG‑CCTGCGGCCCGCCTAA‑CAA‑CTT‑GTCGCC‑GGAGAACCCCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTa                                                                                                                                                                                  <  2:291162/238‑1 (MQ=255)

                                                          ccactgatggctgcgggTGTAAAATAAAGCCCCAAGGTGCTTTCCAGCCCCGGGGGGT‑TCTTTTTACCCCGGCGTACTG‑CCCCTTTCAGCGGGTGC‑TTTGGCAGGGGGGCGATCTTCTCTTTTTTCCCAACAATAATCCCTG‑CCGGCGCCCCGCCTAA‑CAA‑CTT‑GTCGCC‑GGAAAAACCCACCAGCCGGACCCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTa                                                                                                                                                                                  <  2:241154/225‑1 (MQ=255)

                                                              cAGAGTTTCAGGGTGT‑AAAAAAAGACGCAACGGGGCTCCCAGCGCCGCGAGGG‑TCATTTTATCCGCGCGTAATG‑CACGCTTCAGCGGGTGG‑CTTTGCAGGCGGGCGATCATCTCTTTTTTACCAACAATAATTCCTG‑CCTGCGGCCCGCCTAA‑CAA‑CTT‑GTCGCC‑GGAGAAACTCCCCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGc                                                                                                                                                                             <  1:887983/239‑1 (MQ=255)

                                                                 agCTTCAGGGTGT‑AAATAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGG‑TCATTTTATCCGCGCGTAATG‑CACGCTTCAGCGGGTGG‑CTTTGCAGGCGGGCGATCATCTCTTTTTTAACAACAATAATTCCTG‑CCTGCGGCCCGCCTAA‑CAA‑CTT‑GTCGCC‑GGAGAAACTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGATCTTTCGGCAAACCGTACTGGCTaa                                                                                                                                                                          >  1:1048005/1‑239 (MQ=255)

                                                                  gCTTCAGGGTGT‑AAATAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGG‑TCATTTTATCCGCGCGTAATG‑CACGCTTCAGCGGGTGG‑CTTTGCAGGCGGGCGATCATCTCTTTTTTACCAACAATAATTCCTG‑CCTGCGGCCCGCCTAA‑CAA‑CTT‑GTCGCC‑GGAGAAACTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTa                                                                                                                                                                           <  1:1261200/237‑1 (MQ=255)

                                                                        ggggtaaaaaaaaggccGCAAGGGGGCTTCCGGCGCCGGGGGGT‑TCTTTTTACCCGCGCGTATGG‑CCCGCTTCGGGGGGTGG‑TTTGGGGGGCGGGCGATCATCTTTTTTTTCCCAACAATAATTCCTG‑CCGGCGCCCCGCCAAA‑CAA‑CTT‑GTCGCC‑GGAAAAACCCCCCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCg                                                                                                                                                                     <  2:173725/222‑1 (MQ=255)

                                                                         ggTGT‑AAATAAAGACGCAACGTGCCTTCCAGCGCCGCGAGGT‑TCTTTTTACCCGCGCTTAATG‑CACGCTTCAGGGGGTGG‑CTTTGCAGGCGGGCGATCATCTCTTTTTTACAAAAAATAATCCCTG‑CCTGCGCCCCGCCTAA‑CAA‑CTT‑GTCGCC‑GGAGAACCTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGAc                                                                                                                                                                   <  2:129161/238‑1 (MQ=255)

                                                                           tgt‑AAATAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGG‑TCATTTTATCCGCGCGTAATG‑CACGCTTCAGCGGGTGG‑CTTTGCAGGCGGGCGATCATCTCTTTTTTACCAACAATAATTCCTG‑CCTGCGGCCCGCCTAA‑CAA‑CTT‑GTCGCC‑GGAGAAACTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCa                                                                                                                                                                 >  1:871926/1‑238 (MQ=255)

                                                                                  aaagaacgaaccggggCTCCCGGCCCCGCGAGGG‑TTATTTTACCCGCGCTTAATG‑CCCCCTCCGGGGGGTGT‑CTTTGCGGGCGGGCGATCTTCTCTTTTTACCCAACAATAATCCCTG‑CCTGCGCCCCGCCTAA‑CAA‑CTT‑GCCGCG‑GGAAAACCTCACCAGACGGCCGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGcc                                                                                                                                                          <  2:739975/225‑1 (MQ=255)

                                                                                   aaccaCGCAACGTGGTGTCAAGCGGCGAGGGCG‑TTATTTTCCCCGCGGAGAATG‑CCCGCGACAGCGGGGGG‑GTTGGCAGGCGGGCTAACCTCTCTTTATACCCAAAAAGAATGCCTG‑CCTGCCGCGCGCCCAC‑CAA‑CTT‑GCCGCC‑GGAGAACCCCCCCAGACGGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCa                                                                                                                                                         <  2:544867/235‑1 (MQ=255)

                                                                                   aaatccgcatgagggCTCCCGGCGCGGTGAGGG‑TATTTTCATCCGCGAG‑AATGCCCCGCTCCAGGGGGTGG‑CTTTGCGGGCGGGCGATATTCTCTTTTTTCCCAACAA‑AATCCCCGCCCTGCGCCCCGCCTAA‑CAC‑CTT‑GCCGCC‑GGAAAACCCCCCCAGACTGCCGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCa                                                                                                                                                         <  2:952658/226‑1 (MQ=255)

                                                                                   aaaGCCGCACCGTGGCTCCCAGCGCCGCGAGGG‑TCATTTTACCCGCGCGTAATG‑CACGCTTCAGCGGGTGG‑TTTTGCAGGCGGGCGATCATCTCTTTTTTCCCAACAATAATCCCTG‑CCTGCGGCCCGCCTAA‑CAA‑CTT‑GTCGCC‑GGAGAACCTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCa                                                                                                                                                         <  1:850478/239‑1 (MQ=255)

                                                                                    aaagccccaagggggttcccggcccgcGGGGGTTCTTTTTACCCGCCGTTAATG‑CCCGCTTCGGCGGGTGG‑CTTGGCAGGCGGGCGTTCATCTTTTTTTTACCAAAAAAAATCCCTG‑CCCGCGCCCCGCCTAA‑CAA‑CTT‑GCCGCG‑GGAGAAACCCACCAGCCTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCa                                                                                                                                                         <  2:1162896/216‑1 (MQ=255)

                                                                                    aaGACGCAACGTGGCTTCCAGCGCCGCGAGGG‑TCATTTTATCCGCGCGTAATG‑CACGCTTCAGCGGGTGG‑CTTTGCAGGCGGGTGTTCATCTCTTTTTTCCCCACCATAATTCCTG‑CCTCCCGCCCGACCAA‑CAA‑CTT‑TTCCCC‑GGAGCACATCACCAGACTGACGCCCGCCACCAACAAATCCCGGGTAAATGGCTCTTTTGGCAAAACGGACCGGCTAAGacgcgcccacaaccca                                                                                                                                                         >  2:1194505/1‑223 (MQ=255)

                                                                                      gACGCAACGTGGCTTCCAGCGCCGCGAGGG‑TCATTTTATCCGCGCGTAATG‑CACGCTTCAGCGGGTGG‑CTTTGCAGGCGGGCGATCATCTCTTTTTTACCAACAATAATTCCTG‑CCTGCGGCCCGCCTAA‑CAA‑CTT‑GTCGCC‑GGAGAAACTCACCCCACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTCAGATCCACCAGCGAGCCACt                                                                                                                                                       >  2:129739/1‑238 (MQ=255)

                                                                                      gACGCAACGTGGCTTCCAGCGCCGCGAGGG‑TCATTTTATCCGCGCGTAATG‑CACGCTTCAGCGGGTGG‑CTTTGCAGGCGGGCGATCATCCCTTTTTTACCAACAATAATTCCTG‑CCTGCGGCCCGCCTCA‑CAA‑CTT‑GTCGCC‑GGAGAAACTCACCAGAATGACGCCCGCCGCCATCAACTCCTGCGGCATTGGCTCTTTCGGCAAAACGTACTGGGTAAGATCGACCAGCGAGCCACt                                                                                                                                                       >  1:268460/1‑238 (MQ=255)

                                                                                              ggggtctCCCGCGCGGGGGGGG‑GCATTTTACCCGCGGTGAGTG‑CACCGGTGAGGGGGGGT‑CTTTGCGGGGGGGAGACAGTCTCTTTTTCACAAAAAAAATGCCCTC‑CCTGCGCCCCGCCAAA‑AAA‑CTG‑GTCGCG‑GGAGAACCCCCCCAGCCTGCCGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTaaa                                                                                                                                                <  2:259634/231‑1 (MQ=255)

                                                                                                  cTTCCAGGGCGGGGGGGT‑TCATTTTACCCGCGCGTAATC‑CACGCTTGGGCGGGGGG‑CTTGGGAGGCGGGCGATCATCTCTTTTTACCCAACAATAATTCCTG‑CCGGCGCCCCCCATAA‑CAA‑CTT‑GCCGCC‑GGAGAACCTCCCCAGACGGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGt                                                                                                                                           <  2:928817/238‑1 (MQ=255)

                                                                                                      cAGCGCCGCGAGGT‑TCATTTTATCCGCGAGTAAGG‑CACGCTTCAGCGGGTGG‑CTTTGCAGGCGGGCGATCATCTCTTTTTTACCAACAATAATCCCTG‑CCTGCGGCCCGCCTAA‑CAA‑CTT‑GTCGCC‑GGAGAAACCCACCAGACTGCCGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTa                                                                                                                                      <  1:994292/239‑1 (MQ=255)

                                                                                                                  ggttcttttttcccGCGCTTATTG‑CCCGCTTC‑GGGGGTGGTCTTTGCCGGCGGGCAACCATCTTTTTTTTACCAAAAATATTCCCTC‑CCTGCGCCCCGCCAAA‑CAA‑CTT‑GCCGCG‑GGAAAACCTCACCAGACGGCCGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATc                                                                                                                            <  2:1039319/226‑1 (MQ=255)

                                                                                                                    gtgaTTTTATCCGGGGTTAATG‑CCCGCTGAGGGGGGTGG‑CTTTGCGGGCGGGCGATCATCTCTTTTTTACCAAAAATAATTCCTG‑CCGGCGCCCCGCCTAA‑CAA‑CTT‑GTCGCC‑GGAAAAACTCACCAGCCTGCCGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCa                                                                                                                          <  2:645528/235‑1 (MQ=255)

                                                                                                                              ccGCGCGTAATG‑CACGCTTCAGCGGGTGG‑CTTTGCAGGCGGGCGATCATCTCTTTTTTACCAACAATAATTCCTG‑CCTGCGGCCCGCCTAA‑CAA‑CTT‑GTCGCC‑GGAGAAACTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGcc                                                                                                                >  1:173320/1‑238 (MQ=255)

                                                                                                                                 cgcgTAATG‑CACGCTTCAGCGGGTGG‑CTTTGCAGGCGGGCGATCATCTCTTTTTTACCAACAATAATTCCTG‑CCTGCGGCCCGCCTAA‑CAA‑CTT‑GTCGCC‑GGAGAAACTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAgc                                                                                                            >  1:623427/1‑239 (MQ=255)

                                                                                                                                  gcgTAATG‑CACGCTTCAGCGGGTGG‑CTTTGCAGGCGGGCGATCATCTCTTTTTTACCAACAATAATTCCTG‑CCTGCGGCCCGCCTAA‑CAA‑CTT‑GTCGCC‑GGAGAAACTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGGgc                                                                                                            >  1:1095781/1‑238 (MQ=255)

                                                                                                                                   cgTAATG‑CACGCTTCAGCGGGTGG‑CTTTGCAGGCGGGCGATCATCTCTTTTTTACCAACAATAATTCCTG‑CCTGCGGCCCGCCTAA‑CAA‑CTT‑GTCGCC‑GGAGAAACTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCAAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCcgcgcg                                                                                                           >  1:922549/1‑238 (MQ=255)

                                                                                                                                           cACGCTCCGGCGGGTGG‑CTTGGCAGGCGGGCGTTCATCTCTTTTTTACCAAAAATAATCCCTG‑CCGGCGGCCCGCCTAA‑CAA‑CTG‑GTCGCC‑GGAAAACCCCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGt                                                                                                   <  1:211735/239‑1 (MQ=255)

                                                                                                                                               cTTCAGCGGGTGG‑CTTTGCAGGCGGGCGATCCTCTCTTTTTTACCAACAATAATTCCTG‑CCTGCGGCCCGCCTAA‑CAA‑CTT‑GTCGCC‑GGAGAAACTCACCAGACTGACGCCCGCCGCAATCCACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCCAAGATCGACCAGCGCGCCACTGCCTAAATCCGTCACTACCGGAAAATCCCGCTCTTTTCCGCGCGCCACCAGTTcc                                                                                                >  2:575101/1‑238 (MQ=255)

                                                                                                                                                ttcgggggttGG‑CTTTCGGGGGGGGCAATCATTTTTTTTTTCCCAAAAAAAATTCCCC‑CCTGGGCCCCGCCAAA‑CAA‑CTG‑GGCGCC‑GGAGAAACCCCCCAGACGGCCCCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGc                                                                                              <  2:1298644/230‑1 (MQ=255)

                                                                                                                                                 tCAGCGGGTGG‑TTTTGCAGGGGGGCGATCATCTTTTTTTTACCAACAATAATTCCTG‑CCTGCGCCCCGCCTAA‑CAA‑CTT‑GTCGCC‑GGAGAACCTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCt                                                                                             <  1:1216388/239‑1 (MQ=255)

                                                                                                                                                 tCAGCGGGGGG‑CTTTGCGGGCGGGCCATCTTCTCTTTTTCACCAAAAATAATTCCTG‑CCTGCGGCCCGCCTAA‑CAA‑TTT‑GGCGCC‑GAAAAAACTCCCCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCt                                                                                             <  1:302413/239‑1 (MQ=255)

                                                                                                                                                        gTGG‑CTTTGCAGGCGGGCGATCATCTCTTTTTTACCAACAATAATTCCTG‑CCTGCGGCCCGCCTAA‑CAA‑CTT‑GTCGCC‑GGAGAAACTCACCAGACTGACGCCCCCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTATATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGAGCCACCAGTTCCCCTTCATCTa                                                                                      >  1:123471/1‑239 (MQ=255)

                                                                                                                                                          gg‑CTTTGCAGGCGGGCGATCATCTCTTTTTTACCAACAATAATTCCTG‑CCTGCGGCCCGCCTAA‑CAA‑CTT‑GTCGCC‑GGAGAAACTCCCCAGACTGACGCCCCCCCCAATCAACTCCTTCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCCCTTTGCCGAGCGCCACCCGTTCCGCCTCATCAAt                                                                                     >  2:1163637/1‑238 (MQ=255)

                                                                                                                                                          gg‑CTTTGCAGGCGGGCGATCATCTCTTTTTTACCAACAATAATTCCTG‑CCTGCGGCCCGCCTAA‑CAA‑CTT‑GTCGCC‑GGAGAAACTCACCAGACTGCCGCCCGCCGCAATCAACCCCTGCGGCCATGGCTCTTTCGGCCAACCGTACTGGCCAAGATCGACCAGCGAGCCCCTGCCTAAATCAGTCCCCACGGGAACATCCAGCTCTTTGCCGGGCGCCCCCCGTTCCGCCTCCTCTAt                                                                                     >  2:913543/1‑238 (MQ=255)

                                                                                                                                                          gg‑CTTTGCAGGCGGGCGATCATCTCTTTTTTACCAACAATAATTCCTG‑CCTGCGGCCCGCCTAA‑CAA‑CTT‑GTCGCC‑GGAGAAACTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCTGCCAACCGGACTGGCTAAGATCGACCACCGCGCCACTGCCTAAATCACTCACTACGGGAACATCCAGCCCTTTTCCGAGCCCCACCAGTTCCCCTCCATCTAt                                                                                     >  2:1260478/1‑238 (MQ=255)

                                                                                                                                                          gg‑CTTTGCAGGCGGGCGATCATCTCTTTTTTACCAACAATAATTCCTG‑CCTGCGGCCCGCCTAA‑CAA‑CTT‑GTCGCC‑GGAGAAACTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCCGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCCGTTCCGCTTCATCTAt                                                                                     >  1:801389/1‑238 (MQ=255)

                                                                                                                                                             cTTTGCAGGCGGGCGATCATCTCTTTTTTACCAACAATAATTCCTG‑CCTGCGGCCCGCCTAA‑CAA‑ATT‑GTCGCC‑GGAGAAACTCACCAGACTGACGCCCGCCGCAATCCACTCCTTCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCCGCTCTTTGCCGGGCCCCACCCGTTCCGCTTCATCTATc                                                                                    >  2:298689/1‑237 (MQ=255)

                                                                                                                                                                     gggggcgAACACATTTTTTTAAACAAAAATACTCCCCG‑CCGGCGGCCCGCAAAA‑CCA‑TGG‑GGCGCG‑GGAAAATCCCCCCAGACAGCGCCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTg                                                                           <  2:871926/236‑1 (MQ=255)

                                                                                                                                                                       gggcgTTCATCTCTTTTTTCCCAACAATAATCCCTG‑CCGGCGGCCCCCCTAC‑CAA‑CTT‑GTCGCC‑GGAAAACCCCCCCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGaa                                                                         <  1:1317188/238‑1 (MQ=255)

                                                                                                                                                                       gggcgCTCTTCT‑TTTTTTCACAAAAAAAATTCCCGCCCTGCGCCCCCCCAAA‑CAA‑TTG‑GTCGGG‑GGAAAACCCCCCCAGCCTGCCGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGaa                                                                         <  2:273304/238‑1 (MQ=255)

                                                                                                                                                                       gggcgATCCTCTCTTTTTTTCCAACAATAATTCCTG‑CCCGCGGCCCGCCTAA‑CCA‑CTT‑GTCGCC‑GGAGAAACTCACCAGACTGACGCCCGCCGCCATCAACTCCTGCGGCATTGGCTCTTTCGGCCAACCCGACCGGCTAAGATCCACCAGCGAGCCCCCGCCTAAATCCGTCCCCACGGGAACATCCAGCTCCTTTCCGAGCGCCACCAGATCCCCTTCATCTATCGCTTTGGCGaa                                                                         >  2:314966/1‑238 (MQ=255)

                                                                                                                                                                       gggcgATCATCTCTTTTTTACCAACAATAATCCCTG‑CCTGCGGCCCGCATAA‑CAA‑CTT‑GTCGCC‑GGAGAAACTCACCAGCCTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGaa                                                                         <  1:607469/238‑1 (MQ=255)

                                                                                                                                                                       gggcaATCTCCTCTTTTTTACCAACAATATTTCCTC‑CCTGCGGCCCGCCTAA‑CAT‑CTT‑GTCGCC‑GGAGAAACTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGaa                                                                         <  1:1288360/238‑1 (MQ=255)

                                                                                                                                                                           gATCATCTCTTTTTTCCAAACAATATTTCCTG‑CCTGCGGCCCGCCTAA‑CAA‑CTT‑GTCGCC‑GGAGAACCTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCt                                                                    <  1:1314062/239‑1 (MQ=255)

                                                                                                                                                                             tcatcTTTTTTTTACAAAAAAAAATTCCTG‑CCTGCGGCCCGCCTAA‑CAA‑CTG‑GCCGCC‑GGAAAAACCCCCCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGa                                                                  <  1:23588/239‑1 (MQ=255)

                                                                                                                                                                              ctttttttttttCCCAAAAATATATCCGC‑CCGGCGCCCCGCTTAA‑CAC‑CTT‑GCCCCC‑GGAAAAACTCACCAGCCGGCTCCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGaa                                                                 <  2:1012718/233‑1 (MQ=255)

                                                                                                                                                                              cttctcTTTTTCACCAAGAATAATCCGTG‑CCGGCTGCCCACCAAC‑CAA‑CTG‑GGGGCC‑GGAGAACCCCTCCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCGTCATCTATCGCTTTGGTGAACCCCTGaa                                                                 <  1:1105534/237‑1 (MQ=255)

                                                                                                                                                                                ttttttttttACCAAAAATAATCCCT‑‑CCCGCAGGCCCCCCAA‑CAACCTG‑GCCGCC‑GAAGAACCCCCCCAGCCGCCCCCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATg                                                               <  2:1303211/235‑1 (MQ=255)

                                                                                                                                                                                  tctTTTTTACCAAAAATAATCCCCG‑CCTGCGGCCCGCCTAA‑CAA‑CTT‑GTCCCC‑GGAGAAACTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCt                                                             <  1:1260478/239‑1 (MQ=255)

                                                                                                                                                                                  ctttttttACAAAAAAAATTTCCCC‑CCGGC‑GCCCCCCCAA‑CCA‑CTTGGCCGCC‑GGAAAACCCCACCAGACGAACCCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCt                                                             <  2:86723/237‑1 (MQ=255)

                                                                                                                                                                                         tccaaacaaTAATTCCGG‑CCTGCGGCCCGCCTAA‑CAA‑CTG‑GCCGCG‑GGAGAAACCCACCAGCCTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTa                                                      <  1:312203/235‑1 (MQ=255)

                                                                                                                                                                                         tACCAACAATAATTCCTG‑CCTGCGGCCCGCCTAA‑CAA‑CTT‑GTCGCC‑GGAGAAACTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGCTCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCCTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGtt                                                       >  1:629527/1‑238 (MQ=255)

                                                                                                                                                                                         aaCCAACAATAATTCCGG‑CCTGCGGCCCGCCAAA‑CAA‑CTT‑GCCGCC‑GGAGAAACTCACCAGACGGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTa                                                      <  1:768788/238‑1 (MQ=255)

                                                                                                                                                                                          tccaaaaaaaaatcccgcccggcgcCCCGCCTAA‑CAA‑CTT‑CCCGCC‑GGAAAACCCCACCAGCCTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTa                                                      <  2:585189/218‑1 (MQ=255)

                                                                                                                                                                                           tccaacaaattctcccggccggcgCCCGGCTAACCAC‑CTT‑TTCGCC‑GGAAAACCTCACCAGCCTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTa                                                      <  1:613081/216‑1 (MQ=255)

                                                                                                                                                                                           ccAACAATACTCCCTG‑CCTGCGGCCCTCCTAA‑CAA‑CTT‑GTCGCC‑GGAGAAACTCACCAGACTGCCGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACt                                                    <  1:710829/239‑1 (MQ=255)

                                                                                                                                                                                              acaaTAATTCCTG‑CCTGCGGCCCGCCTAA‑TAA‑CTT‑GTTATC‑GGAGAAACTCACCAGACGGACGTCCGCAGGAATTAACTCCTAATCCATTGGCTCTTTCGGCGAACCGTAGTGGCTAAGGTCGACCAGCGAGCCACTGTCTAAATCAGTTAATACGGTAACATCCAGCCCTTTGACGCGAGCTACCAGTTCCGCTTCATCTATCGCTTTGGTGCACCCATGAATGCTGATGTTACTgg                                                  >  2:1042952/1‑238 (MQ=255)

                                                                                                                                                                                              acaaTAATTCCTG‑CCTGCGGCCCGCCTAA‑CAA‑CTT‑GTCGCC‑GGAGAAACTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGt                                                 >  2:684446/1‑239 (MQ=255)

                                                                                                                                                                                              acaaTAATTCCTG‑CCTGCGGCCCGCCTAA‑CAA‑CTT‑GTCGCC‑GGAGAAACTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGt                                                 >  1:378570/1‑239 (MQ=255)

                                                                                                                                                                                              acaaTAATTCCTG‑CCTGCGGCCCGCCTAA‑CAA‑CTT‑GTCGCC‑GGAGAAACTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGt                                                 >  2:1064392/1‑239 (MQ=255)

                                                                                                                                                                                              acaaTAATTCCTG‑CCTGCGGCCCGCCTAA‑CAA‑CTT‑GTCGCC‑GGAGAAACTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGt                                                 >  1:1166611/1‑239 (MQ=255)

                                                                                                                                                                                                aataatCCCTC‑CCTGCGCCCCCCCAAC‑CAC‑CTT‑CGCGCC‑GGACACACCCCCCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGCCCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTAt                                               <  2:1095781/239‑1 (MQ=255)

                                                                                                                                                                                                   aatccgtg‑cttgcGGCCCGCCTAA‑CAA‑CTT‑GTCGCC‑GGAGAAACTCCCCATACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTa                                            <  1:1236876/233‑1 (MQ=255)

                                                                                                                                                                                                    atccccccccgcccgCCCGCCAAA‑AAA‑CTG‑GCCGCG‑GAAAAACCCCACCGGCCGCACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTa                                            <  2:748248/225‑1 (MQ=255)

                                                                                                                                                                                                    aatcccgccctgcGGCCCGCCTAA‑CAT‑CTT‑GTCGCC‑GGAGAAACTCCCCAGCCTGCCCCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTa                                            <  1:1163637/231‑1 (MQ=255)

                                                                                                                                                                                                     tccccg‑ccggcGCCCCGCCTAA‑CAC‑CTT‑GTGCCC‑GGAAAAACCCCCCAGAGTGCCGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTACt                                          <  2:623427/234‑1 (MQ=255)

                                                                                                                                                                                                       cccgccccgcGGCCCCCCCAA‑CAA‑CCT‑GGCCCCGGGAAACACCCCCCAGACGGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTACt                                          <  2:227702/231‑1 (MQ=255)

                                                                                                                                                                                                        ctg‑cctgcCGCCGGCGTAA‑CAA‑CTT‑CTGTAC‑GGAGAAACTCACCGGACAGACCCCAGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAAACGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCATTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTACTTtc                                       <  1:1238803/239‑1 (MQ=255)

                                                                                                                                                                                                          g‑cctgcGGCCCGCCTAA‑CAA‑CTT‑GTCGCC‑GGAGAAACTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTACTTtca                                      >  2:370333/1‑238 (MQ=255)

                                                                                                                                                                                                          g‑cctgcGGCCCGCCTAA‑CAA‑CTT‑GTCGCC‑GGAGAAACTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTACTTtca                                      >  1:455080/1‑238 (MQ=255)

                                                                                                                                                                                                          g‑cctgcGGCCCGCCTAA‑CAA‑CTT‑GTCGCC‑GGAGAAACTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTACTTtca                                      >  2:72714/1‑238 (MQ=255)

                                                                                                                                                                                                              tgcGGCCCGCC‑AA‑CAACCTT‑GTCGCC‑GGAGAACCTCCCCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTACTTTCATCaa                                  <  1:705759/239‑1 (MQ=255)

                                                                                                                                                                                                                cGGCCCGCCTAA‑CAA‑CTT‑GTCGCC‑GGAGAAACTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTACTTTCATCAACa                                >  2:964038/1‑239 (MQ=255)

                                                                                                                                                                                                                 ggCCCGCCTAA‑CAA‑CTT‑GTCGCC‑GGAGAAACTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTACTTTCATCAACAg                               >  2:489200/1‑239 (MQ=255)

                                                                                                                                                                                                                    ccGCCTAA‑CAA‑CTG‑GTCGCC‑GGAAAAACCCACCAGATGGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTACTTTCATCAACAGTg                             <  1:522790/238‑1 (MQ=255)

                                                                                                                                                                                                                         cccaaccaactgcgcGCA‑GGAGAACCCCACCAAGCCGGCACCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTACTTTCATCAACAGTGCgg                          <  2:450317/225‑1 (MQ=255)

                                                                                                                                                                                                                                     gTCGCC‑GGAGAAACTCCCCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTa                                                                                                                                                                                  <  2:606939/75‑1 (MQ=255)

                                                                                                                                                                                                                                     gTCGCC‑GGAGAAACTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTACTTTCATCAACAGTGCGGTATTttcattc               >  1:454821/1‑238 (MQ=255)

                                                                                                                                                                                                                                        gCC‑GGAGAAACTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACCTCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGCAGTTACTGGTATGTCCTTTCTTCAACAGTGCGGGATTttcattccgc            >  2:214601/1‑235 (MQ=255)

                                                                                                                                                                                                                                          cgggAGAAACCCCCCGGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTACTTTCATCAACAGTGCGGTATTTTCATTCACCGc          <  1:472381/237‑1 (MQ=255)

                                                                                                                                                                                                                                             gagaAACTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTACTTTCATCAACAGTGCGGTATTTTCATTCACCGCCt        <  1:489200/238‑1 (MQ=255)

                                                                                                                                                                                                                                                  acccACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCGCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCTCCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTACTTTCATCAACAGTGCGGTATTTTCATTCACCGCCTGACGAt  <  1:69543/236‑1 (MQ=255)

GCCTGGATTTGAATGACCTCTGCGCTGCGGGTAAGCAGGCGCAGGGTCGGTAATTTTTCACTCAGAGCTTCAGGGTGT‑AAATAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGG‑TCATTTTATCCGCGCGTAATG‑CACGCTTCAGCGGGTGG‑CTTTGCAGGCGGGCGATCATCTCTTTTTTACCAACAATAATTCCTG‑CCTGCGGCCCGCCTAA‑CAA‑CTT‑GTCGCC‑GGAGAAACTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTACTTTCATCAACAGTGCGGTATTTTCATTCACCGCCTGACGAT  >  NC_000913/3760146‑3760617

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`