| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 4,151,322 | A→G | 15.9% | A592A (GCT→GCC) | ppc ← | phosphoenolpyruvate carboxylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,151,322 | 0 | A | G | 15.9% | 145.8 / 3.7 | 69 | A592A (GCT→GCC) | ppc | phosphoenolpyruvate carboxylase |
| Reads supporting (aligned to +/- strand): ref base A (41/14); new base G (6/6); total (49/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.60e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
GCCGCGGTAGACATCGCAGGAGATGACTGACAGTTCATCCATAATGCGACGCCAGCTCTCTTTCGGCTCCGGCGGTGGCAGCAGGTTGGCTTCCAGAATCGCCCCGGTATAAAGCGACAGGCTGCTGACGGTGATTTCTGGCAGACCATATTTAAA‑GCGGATCATCTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGCGGTTGTGACAGCAGCGCCGCATGAG‑CA‑GGTGCGCCGCCGCGACCAA‑TGGA‑ACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATT‑GCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGCCCTGAATCAGGCCACGATACCAGTCAATATTGAGCAGCTGGGTCATGACATCGTTGG > NC_000913/4151088‑4151557 | gCCGCGGTAGACATCGCAGGAGATGACTGACAGTTCATCCATAATGCGACGCCAGCTCTCTTTCGGCTCCGGCGGTGGCAGCAGGTTGGCTTCCAGAATCGCCCCGGTATAAAGCGACAGGCTGCTGACGGTGATTTCTGGCAGACCATATTTAAA‑GCGGATCATCTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGCGGTTGTGACAGCAGCGCCGCATGAG‑CA‑g > 2:786226/1‑239 (MQ=255) gACATCGCAGGAGATGACTGACAGTTCATCCATAATGCGACGCCAGCTCTCTTTCGGCTCCGGCGGTGGCAGCAGGTTGGCTTCCAGAATCGCCCCGGTATAAAGCGACAGGCTGCTGACGGTGATTTCTGGCAGACCATATTTAAA‑GCGGATCATCTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGCGGTTGTGACAGCAGCGCCGCATGAG‑CA‑GGTgcggcg > 2:825669/1‑238 (MQ=255) cGCAGGAGATGACTGACAGTTCATCCATAATGCGACGCCAGCTCTCTTTCGGCTCCGGCGGTGGCAGCAGGTTGGCTTCCAGAATCGCCCCGGTATAAAGCGACAGGCTGCTGACGGTGATTTCTGGCAGACCATATTTAAA‑GCGGATCATCTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGCGGTTGTGGCAGCAGCGGCGCATGGG‑CA‑GGTGCGCCGCcgcg > 2:937768/1‑238 (MQ=255) aTGACTGACAGTTCATCCATAATGCGACGCCAGCTCTCTTTCGGCTCCGGCGGTGGCACCAGGTTGGCTTCCAGAATCGCCCCGGCATAAAGCGACAGGCTGCTGACGGTGTTTTCTGGCAGACCATATTTAAA‑GCGGATCATCCCGCCCTGTTCGGGTACGCGCAGCCCGCCTTTCAGGCCTCCTGGCGGGTGTGACCGCATCCCCCCATTGT‑CA‑GGCGcgccccccccgccacacgg > 1:1025937/1‑227 (MQ=255) gactgacAGTTCATCCATAATGCGACGCCAGCTCTCTTTCGGCTCCGGCGGTGGCAGCAGGTTGGCTTCCAGAATCGCCCCGGTATAAAGCGACAGGCTGCTGACGGTGATTTCTGGCAGACCATATTTAAA‑GCGGATCATCTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGCGGTTTTGACAGCAGCGCCGCATGGG‑CA‑GGTGCGCCGCCGCCACCAA‑TGGa‑a > 2:40720/1‑238 (MQ=255) gactgacAGTTCATCCATAATGCGACGCCAGCTCTCTTTCGGCTCCGGCGGTGGCAGCAGGTTGGCTTCCAGAATCGCCCCGGTATAAAGCGACAGGCTGCTGACGGTGATTTCTGGCAGACCATATTTAAA‑GCGGATCATCTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGCGGCTGTGACAGCAGCGCCGCATGAG‑CA‑GGTGCGCCGCCGCGACCAA‑TGGa‑a > 2:810727/1‑238 (MQ=255) tgacAGTTCATCCATAATGCGACGCCAGCTCTCTTTCGGCTCCGGCGGTGGCAGCAGGTTGGCTTCCAGAATCGCCCCGGTATAAAGCGACAGGCTGCTGACGGTGATTTCTGGCAGACCATATTTAAA‑GCGGATCATCTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGCGGTTGGGACAGCAGCGCCGCATGAG‑CA‑GGGGCGCCGCCGCGACCAA‑TGGA‑Accg > 2:496399/1‑238 (MQ=255) acAGTTCATCCATAATGCGACGCCAGCTCTCTTTCGGCTCCGGCGGTGACAGCAGGTTGGCTTCCAGAATCGCCCCGGTATAAAGCGACAGGCTGCTGACGGTGATTTCTGGCAGACCATATTTAAA‑GCGGATCATCTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTCGCGGGTGTGGCAGCAGCCCCGCCTCGG‑CA‑AGATCGCCGCCCCGGCCAA‑TGGacacacc > 1:111395/1‑232 (MQ=255) aCGCCAGCTCTCTTTCGGCTCCGGCGGTGGCAGCAGGTTGGCTTCCAGAATCGCCCCGGTATAAAGCGACAGGCTGCTGACGGTGATTTCTGGCAGACCATATTTAAA‑GCGGATCATCTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGCGGTTTTGACAGCAGCGCCGCATGAG‑CA‑GGGGCGCCGCCCCGACCAA‑TGGG‑AACGCCGCGGCCGGGGACCAACGTCa > 2:268179/1‑239 (MQ=255) aCGCCAGCTCTCTTTCGGCTCCGGCGGTGGCAGCAGGTTGGCTTCCAGAATCGCCCCGGTATAAAGCGACAGGCTGCTGACGGTGATTTCTGGCAGACCATATTTAAA‑GCGGATCATCTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGCGGTTGTGACAGCAGCGCCGCATGAG‑CA‑GGTGCGCCGCCGCGACCAA‑TGGA‑ACCGCCGCGACCGGGGGACAACGTc > 2:124247/1‑238 (MQ=255) cGCCAGCTCTCTTTCGGCTCCGGCGGTGGCAGCAGGTTGGCTTCCAGAATCGCCCCGGTATAAAGCGACAGGCTGCTGACGGTGATTTCTGGCAGACCATATTTAAA‑GCGGATCATCTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGCGGTTGTGACAGCAGCGCCGCATGGG‑CA‑GGTGCGCCGCCGCGACCAA‑TGGA‑ACCGCCGCGACCGTGGGACAACGTcc > 2:894230/1‑237 (MQ=255) cGCCAGCTCTCTTTCGGCTCCGGCGGTGGCAGCAGGTTGGCTTCCAGAATCGCCCCGGTATAAAGCGACAGGCTGCTGACGGTGATTTCTGGCAGACCATATTTAAA‑GCGGATCATCTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGCGGTTGTGACAGCAGCGCCGCATGCG‑CA‑GGTGCGCCGCCGCGACCAA‑TGGG‑ACCGCCCCGACCGCGGAACAACGTCa > 2:378021/1‑238 (MQ=255) ttCGGCTCCGGCGGTGGCAGCAGGTTGGCTTCCAGAATCGCCCCGGTATAAAGCGACAGGCTGCTGACGGTGATTTCTGGCAGACCATATTTAAA‑GCGGATCATCTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGCGGTTGTGACAGCAGCGCCGCATGAG‑CA‑GGTGCGCCGCCGCGACCAA‑TGGA‑ACCGCCGCGACCGTGGAACAACGTCAGCTCAATAacc > 2:70284/1‑237 (MQ=255) gCTCCGGCGGTGGCAGCAGGTTGGATTCCAGCATCGCCCCGGTATCAAGCGACAGGCTGCTGACGGTGATTTCTGGCAGACCATATTTAAA‑GCGGATCATCTCGCCCCGTTCGGTTACTCGCAGGCCGCCTTTCAGGCTTCCTGGCGGTTGTGACAGCAACAGCCCATCAG‑CA‑CGCGCGCCGGCCGGAACAA‑TGGG‑ACCGCCGCGCCCCTGGAACAACGGACGCTCGACACCCGCtgtt > 1:575590/1‑239 (MQ=255) gggcggTGGCGGGAGTTTGGCTGCAAAACCCCCCCGGGTAAAAGGCGACGGGCTGGTGGCGGTTTTTTGTGGCATA‑CATTTTTAAAGGCGGATCATCCCCCCCTTTGCGTTTCCGCGGGGGCCCCTTTTCGGGTTTCGGGGGGGTTGGAAAGGCGGCGCC‑CATGGG‑GAGGGGGCCCCCCCGCCACCAA‑TGGA‑ACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCg < 1:236658/237‑1 (MQ=255) cggcggTGGCAGGGGGTTGGCTTCCAGAATCCCCCGGGTAAAACGCGACAGTCTGCGGCGGGTGTTTTCGGGCAGACCATTTTAAAG‑GCGGTTCTTCTCGCCTTGTTCGGTCACGCGCGGGCCGCCTTTCGGGCTTCCGGGGGGTTGTGACCGCAGCCCCGCAGGAG‑CG‑GGCGCCCCCCCGCGACCAA‑TGAA‑ACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCg < 1:769381/238‑1 (MQ=255) ggcggTGGCAGCAGGTTGGCTTCCAGAATCGCCCCGGTATAAAGCGACAGGCTGCTGACGGTGATTTCTGGCAGACCATATTTAAA‑GCGGATCATCTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGCGGTTGTGGCAGCACCGCCGCATGAG‑CA‑GGGGCGCCGCCGCGGCCAA‑TGGA‑ACCGCCGCCGCCGTGGGACAACGTCAGCCCAAAACCCCCTTTTTCGCa > 2:621207/1‑239 (MQ=255) ggcggTGGCAGCAGGTTGGCTTCCAGAATCGCCCCGGTATAAAGCGACAGGCTGCTGACGGTGATTTCTGGCAGACCATATTTAAA‑GCGGATCATCTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGCGGGTTTGACCGCAGCGCCGCATGGGACA‑GG‑GCGCCGCCCCCACCCA‑TGGA‑ACCCCCCCGGCCCTGGAACCACGTCAGCCCAAAATCCCCttttttccc > 1:249472/1‑235 (MQ=255) tggagtttGGCTGCCAAAGTCGCCCTGTTAAAAGGCGAGGGGCGGCGGCCGGTGTTTTCTGCCAGCCCATTTTTAAA‑CGGGTTCACCCCCCCTGGTTCGGTAGCGCCCGGCCCTCTTTCCGGGCTCCCTGGCGTTTGTGAGGGCGGCGCCGCATGAG‑AG‑GGTGCCCCGCCGCGACCAA‑TGGA‑ACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGa < 1:172746/232‑1 (MQ=255) cagGTTGGCTTCCAGAATCGCCCCGGTATAAAGCGACAGGCTGCTGACGGTGATTTCTGGCAGACCATATTTAAA‑GCGGATCATCTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGCGGTTGTGACAGCAGCGCCGCATGAG‑CA‑GGTGCGCCCCCGCGACCAA‑TGGA‑ACCGCCGCGACCGTGGAACAACGTCAGCTCCATACCCGCCTTTTCGCCGGTTTTGATTa > 2:663099/1‑239 (MQ=255) ggCTTCCAGAATCGCCCCGGTATAAAGCGACAGGCTGCTGACGGTGATTTCTGGCAGACCATATTTAAA‑GCGGATCATCTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGCGGTTGTGACAGCAGCGCCGCATGAG‑CA‑GGTGCGCCGCCGCGACCAA‑TGGA‑ACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCa > 2:299960/1‑238 (MQ=255) ccccGGTATAAAGCGACAGGCTGCTGACGGTGATTTCTGGCAGACCATATTTAAA‑GCGGATCATCTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGCGGTTGTGACAGCAGCGCCGCATGAG‑CA‑GGTGCGCCGCCGCGACCAA‑TGGA‑ACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGatat > 2:543359/1‑239 (MQ=255) aaaaaGCGAGAGGCTGCGGAGGGTGATCTCTGTCAGACATTTTTTAAA‑CCGGTCCATCCCGCTGTTTCCGTTCACCCGCAGGCCCCCTTTCAGGCTTCCTGGCGGTTGGGACCGCAGCGCCGCAGGAG‑AG‑GGGGCGCCCCCGCAACAAG‑TGGA‑ACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATT‑GCGccc < 1:360996/237‑1 (MQ=255) aCAGGCTGCTGACGGTGATTTCTTGCAGACCATATTTAAA‑GCGGATCATCTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGCGGCTGTGAAAGCAGCGCCGCATGAG‑CC‑AGTGCGCCGCCTCGCCCAA‑TGGA‑AACGCCGCGACCGTGGAACAACGGCAGCTCAATACCCGCCCTTTTGCCGGGTTTGCTTAAATCCTCCTTCGCCTGATCTTCGCCCCCAgaa > 1:594373/1‑234 (MQ=255) aCAGGCTGCTGACGGTGATTTCTGGCAGACCATATTTAAA‑GCGGATCATCTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGCGGTTGTGACAGCAGCGCCGCATGAG‑CA‑GGTGCGCCGCCGCGACCAA‑TGGA‑ACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATT‑GCGCCCAGGaa > 2:108804/1‑236 (MQ=255) cAGGCTGCTGACGGTGATTTCTGGCAGACCATATTTAAA‑GCGGATCATCTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGCGGTTGTGTCAGCAGCGCCGCATGAG‑CA‑GGTGCGCCGCCGCGACCAA‑TGGA‑ACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATT‑GCGCCCAGGAAGCt > 2:738891/1‑238 (MQ=255) cAGGCTGCTGACGGTGATTTCTGGCAGACCATATTTAAA‑GCGGATCATCTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGCGGTTGTGACAGCAGCGCCGCATGAG‑CA‑GGTGCGCCGCCGCGACCAA‑TGGA‑ACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATT‑GCGCCCAGGAAGCt > 2:820179/1‑238 (MQ=255) cAGGCTGCTGACGGTGATTTCTGGCAGACCATATTTAAA‑GCGGATCATCTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGCGGTTGTGACAGCAGCGCCCCATGAG‑CA‑GGGGCGCCGCCCCGACCAA‑TGGA‑ACCGCCGCGGCCGGGGAACAAAGTCATCTCAATACCCGCTTTTTCTCAAGTTTTTATTAATGCATCCTTGTCCTGAGATT‑GCGCCCAGGAAgat > 1:959667/1‑236 (MQ=255) cAGGCTGCTGACGGTGATTTCTGGCAGACCATATTTAAA‑GCGGATCATCTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGCGGTTGTGACAACAGCGCCGCATGAG‑CA‑GGTGCCCCGCCGCGACCAA‑TGGA‑ACCGCCGCGACCGTGGAACAACGTCAGCACAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATT‑GCGCCCAGGAAGCt > 2:286352/1‑238 (MQ=255) cAGGCTGCTGACGGTGATTTCTGGCAGACCATATTTAAA‑GCGGATCATCTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGCGGCTGTGACCGCAGGGCCGCATGCA‑CC‑AGTGCGCCCCCGCGGCCAA‑TGGA‑ACCCCCCGGACCGTGGAAACACATCACGTTCATCCCCGCTTCTTCGCACGGTTTGTTTAATGCGACCTGTCCCTGttaagccacccagccatct > 1:722626/1‑222 (MQ=255) gctgctGACGGTGATTTCTGGCAGACCATATTTAAA‑GCGGATCATCTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGCGGTTGTGACAGCAGCGCCGCATGAG‑CA‑GGTGCGCCGCCGCGACCAA‑TGGA‑ACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATT‑GCGCCCAGGAAGCTGCCa > 2:448614/1‑239 (MQ=255) aCGGTGATTTCTGGCAGACCATATTTAAA‑GCGGATCATCTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGCGGTTGTGACAGCAGCGCCGCATGAG‑CA‑GGTGCGCCGCCGCGACCAA‑TGGA‑ACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATT‑GCGCCCAGGAAGCTGCCATCACTc > 2:828524/1‑238 (MQ=255) gTGATTTCTGGCAGACCATATTTAAA‑GCGGATCATCTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGCGGTTGTGAAAGCCGCGCCGCATGAG‑CG‑GGGGCGCCGCCGCGACCAA‑TGGA‑ACCGCCGCGACCGCGGAAAAACGTCAGCTCAATACCCGCTTTTTCCCAGGTTTTGATTAATGCATCCTGTGCCTGATATT‑GCGCCCAGGAAGCTGCCATCACTCCCg > 1:733390/1‑238 (MQ=255) cATATTTAAA‑GCGGATCATCTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGCGGTTGTGACAGCAGCGCCGCATGAG‑CA‑GGTGCGCCGCCGCGACCAA‑TGGA‑ACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATg > 2:1020930/1‑177 (MQ=255) tatTTAAA‑GCGGATCATCTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGCGGTTGTGACAGCAGCGCCGCATGAG‑CA‑GGTGCGCCGCCGCGACCAA‑TGGA‑ACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATT‑GCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGa > 2:837849/1‑239 (MQ=255) aaa‑GCGGCTCCTCTCCCCGTGTTGGTTGCCGCGCAGCCCCCCTTTCGGCTTTCGTGGCGTTTGGGGCGGGAGCGCCGAAGGGG‑CA‑GGTGCCCCCCCCCGCCCAA‑GGGA‑TCCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATT‑GCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGc < 1:738891/239‑1 (MQ=255) aa‑GCGGATCATCTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGCGGTTGTGACAGCAGCGCCGCATGAG‑CA‑GGTGCGCCGCCGCGACCAA‑TGGA‑ACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATT‑GCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGcc > 2:284041/1‑239 (MQ=255) a‑GCGGATCATCTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGCGGTTGTGACAGCAGCGCCGCATGAG‑CA‑GGTGCGCCGCCGCGACCAA‑TGGA‑ACCGCCGCGACCGGGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATT‑GCGCCCAGGAAGCTTCCATGACTCCCGCACCTTTTGCTGCGTCGGAATAGCCa > 2:919220/1‑239 (MQ=255) gCGGATCATCTCGCCCTGTTCGGTTACGCTCAGGCCGCCTTTCAGGCTTCCTGGCGGTTGTGACAGCAGCGCCGCATGAG‑CA‑GGTGCGCCGCCGCGACCAA‑TGGA‑ACCGCCGCGACCGTGGAACAACGTCAGCTCAATAACCGCTTTTTCGCAGGTTTTGTTTAATGCATCCTGTGCCTGATATT‑GCGCCCAGGAAACTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCaa > 1:997947/1‑239 (MQ=255) gCGGATCATCTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGCGGTTGTGACAGCAGCGCCGCATGAG‑CA‑GGTGCGCCGCCGCCACCAA‑TGGA‑ACCGCCGCGACCGTGGAACAACGTCCGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATT‑GCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCaa > 2:199068/1‑239 (MQ=255) ggATCATCTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGCGGTTGTGACAGCAGCGCCGCATGAG‑CA‑GGTGCGCCGCCGCGACCAA‑TGGA‑ACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCCGGTTTTGATTAATGCATCCTGTGCCTGATATT‑GCGCCCCGGAAGCTGCCATCACTCCCCCCTCTTTTGCTGAGTCGGAATAGCCAatc > 1:107746/1‑239 (MQ=255) atcacccccccttGTTGGGTTCCGCGCGGGCCGCCTTTCGGGCTTCCGGGCGGTGGTGACAGCGCCGCCGCGTGAG‑CG‑GGTGCGCCGCCGCGACCAA‑TGAA‑ACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATT‑GCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAatcat < 1:284041/227‑1 (MQ=255) cccccccTTTGCGTTTCCGCGCAGCCCCCCTTCCAGGCTCCCTGGGGTTTGGCACAGCAGCGCCGCTGGAG‑CA‑GGTCCCCCCCCGCCACAAA‑TGGA‑ACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATT‑GCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCa < 1:512297/235‑1 (MQ=255) cccTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGCGGTTGTGACAGCAGCCCCGCATGAG‑CA‑GGTGCGCCGCCGCGACCAA‑TGGT‑ACCGCCGCGACCGTGGCACAACGTCAGCTCAATACCCGCTTTTTCGCAGGGTTTGATTAATGCTTCCTGTGCCTGATATT‑GCGCCCCGGACGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCa > 1:606448/1‑235 (MQ=255) ccTGTTGGGTCACCGGGAGCCCGCCTTTCGGGTCCCGGGGGGGTTGTGACAGCAGCGGCCAATGAG‑CA‑GGGGCGCCGCCGCGCCCAA‑TGGA‑ACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATT‑GCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGt < 1:825669/239‑1 (MQ=255) acTGTGCGGTTCCGCGCAGGCCGCTTTTGGGGTTCCGTGGGGGTGGGGACAGCAGCGCCGCAGGAG‑AA‑GGTGCGCCGCCGCAACCAA‑GGGA‑ACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATT‑GCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGt < 2:111395/238‑1 (MQ=255) gTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGCGGTTGTGACAGCAGCGCCGCATGAG‑CA‑GGTGCGCCGCCGCGACCAA‑TGGA‑ACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATT‑GCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGttt > 2:933111/1‑238 (MQ=255) ttCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGCGGTTGTGACAGCAGCGCCGCATGAG‑CA‑GGTGCGCCGCCGCGACCAA‑TGGA‑ACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATT‑GCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTg > 2:215190/1‑238 (MQ=255) ttCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGCGGTTGTGACAGCAGCGCCGCATGAG‑CA‑GGTGCGCCGCCGCGACCAA‑TGGA‑ACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATT‑GCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTg > 2:1191112/1‑238 (MQ=255) ttCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGCGGTTGTGACAGCAGCGCCGCATGAG‑CA‑GGTGCGCCGCCGCGACCAA‑TGGA‑ACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGGGCCTGATATT‑GCGCCCAGGAAGATGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTg > 2:256064/1‑238 (MQ=255) tgtgaataggggccagccTTCCAGCCTTCCGGGGGGTTGGGACAGCGGCCCCGCGTGAG‑AG‑GGTGCCCCCCCGCGACAAA‑GGGA‑ACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATT‑GCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGcc < 1:117466/222‑1 (MQ=255) cgcgCAGGCCGCCTTTCAGGCTTCCTGGCGGTTGTGACAGCAGCGCCGCATGAG‑CA‑GGTGCGCCGCCGCGACCAA‑TGGA‑ACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATT‑GCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGCCCTGAATc > 1:362766/1‑239 (MQ=255) cgcAGGCCGCCTTTCAGGCTTCCTGGCGGTTGTGACAGCAGCGCCGCATGAG‑CA‑GGTGCGCCGCCGCGACCAA‑TGGA‑ACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATT‑GCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGCCCTGAATCAg > 2:890169/1‑239 (MQ=255) cgcAGGCCGCCTTTCAGGCTTCCTGGCGGGTGTGACAGCAGCGCCCCATGAG‑CA‑GGTGCGCCGCCGCGACCAA‑AGGA‑ACCGCCGCGACCGTGGAACAACGCCAGCTCCATACCCGCTTTTTCGCCGGTTTTTGTTAATGCATCCTGGGCCTGATATT‑GCGCCCAGCACGCTGCCATCACTCCCGCATCTTTTGCTCAGTTGGAGTAGCCCATCCTCACCCTCTGTTTGACCTGAATCa > 1:880596/1‑238 (MQ=255) ggaGGCCGCCTTTGAGGCTTCCTGGCGGTTGAGACAGCAGCGCCGCATGAG‑GA‑GGTGCGCCTCCGCGACAAA‑TGGA‑ACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATT‑GCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGCCCTGAATCAg < 2:1025937/236‑1 (MQ=255) cccgccTTTCCGGCTCCCGGGCGTTTGGGCCGGCAGCGCGGAATGGG‑CA‑GGTGCGCCCCCGCGACCAA‑TGGA‑CCCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATT‑GCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGCCCTGAATCAGGCCa < 1:215190/237‑1 (MQ=255) acccctgtagtgtctccgtGGCGGTTGTGCCAGCAGCGCGGTAGGGG‑CA‑GGTGCGCCCCCGCGACCAA‑TGGA‑ACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATT‑GCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGCCCTGAATCAGGCCa < 1:837849/220‑1 (MQ=255) ccTTTCAGGCTTCCTGGCGGTTGTGACAGCAGCGCCGCATGAG‑CA‑GGTGCGCCGCCGCGACCAA‑TGGA‑ACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATT‑GCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGCCCTGAATCAGGCCACGAt > 2:1057350/1‑238 (MQ=255) tCAGGCTTCCTGGCGGTTGTGACAGCAGCGCCGCATGAG‑CA‑GGTGCGCCGCCGCGACCCA‑TGGA‑ACCGCCCCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGGAGGTTTTGATTAATGCATCCTGTGCCCGATATT‑GCTCCCAGGAAACTGCCATCACTCCCGCATATTTTGCTGAGTCGGAAAAGCCCATCATCACCAACTGTTTGCCCTGAATCAGGCCCCGATAcac > 1:704497/1‑236 (MQ=255) ctggCTTCCGGGGGGTTGTGACAGAAGCGCCGCAGGAG‑AA‑GGTGCGCCGCCGCGACAAA‑TGGA‑ACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATT‑GCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGCCCTGAATCAGGCCACGATACCAGt < 2:798146/237‑1 (MQ=255) cgggCTTCGGGGCGTTGGGGACAGGCGCGCCGCATGAG‑GG‑GGGGCGCCGCCGCAACCAA‑TGAA‑ACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATT‑GCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGCCCTGAATCAGGCCACGATACCAGt < 1:543359/237‑1 (MQ=255) caggccgcgggggggtggagACAGGCGCGGCGCAGGAG‑CG‑GGTGCCCCGCCGC‑ACCAAGGGGA‑ACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATT‑GCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGCCCTGAATCAGGCCACGATACCAGt < 1:1057350/220‑1 (MQ=255) tCCTGGCGGTTGTGACAGCAGCGCCGCATGAG‑CA‑GGTGCGCCGCCGCGACCAA‑TGGA‑ACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATT‑GCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGCCCTGAATCAgg > 2:117466/1‑220 (MQ=255) tgggaagagcggcGCCCAAGGAG‑CA‑GGTGCCCCGCCGCCCCAAA‑TGGA‑ACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATT‑GCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGCCCTGAATCAGGCCACGATACCAGTCAATATTGagcagc < 2:704497/231‑1 (MQ=255) ggtgccggcagcCCCGCATAGG‑AG‑GGTGCCCCGCC‑CGACCAA‑AGGACACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATT‑GCGCACAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGCCCTGAATCAGGCCACGATACCAGTCAATATTGAGCAGCt < 1:806849/231‑1 (MQ=255) tgACCGCAGCCCCGCATGAG‑CA‑GGTGCGCCCCCGCGGCCAA‑TGGA‑ACCGCCGGGCCCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGAGCCTGATATT‑GCGCCCAGGAAGCTGCCATCCATCGCGCATCCTTTGACGCGTCGGACTAGCCAATCATCATCCTCTGTTTGCCCTGAATCAGGCCACGATACTAGTCACTATTTAGCAGCCggg > 1:1010164/1‑239 (MQ=255) agcGCCGCATGAG‑CA‑GGTGCGCCGCCGCGACCAA‑TGGA‑ACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATT‑GCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCCTCACCATCTGTTTGCCCTGAATCAGGCCACGATCCCAGTCAATTTTGAGCAGCTTGGTCCTGAc > 1:977495/1‑239 (MQ=255) cgcAGGGG‑CA‑GGTGCCCCGCCGCGACCAA‑TGAA‑ACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATT‑GCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGCCCTGAATCAGGCCACGATACCAGTCAATATTGAGCAGCTGGGTCATGACATCGt < 2:107746/239‑1 (MQ=255) aTGAG‑CG‑GGTGCGCCGCCGCGTCCAA‑GGGG‑ACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATT‑GCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGCCCTGAATCAGGCCACGATACCAGTCAATATTGAGCAGCTGGGTCATGACATCGTTgg < 2:575590/239‑1 (MQ=255) | GCCGCGGTAGACATCGCAGGAGATGACTGACAGTTCATCCATAATGCGACGCCAGCTCTCTTTCGGCTCCGGCGGTGGCAGCAGGTTGGCTTCCAGAATCGCCCCGGTATAAAGCGACAGGCTGCTGACGGTGATTTCTGGCAGACCATATTTAAA‑GCGGATCATCTCGCCCTGTTCGGTTACGCGCAGGCCGCCTTTCAGGCTTCCTGGCGGTTGTGACAGCAGCGCCGCATGAG‑CA‑GGTGCGCCGCCGCGACCAA‑TGGA‑ACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATT‑GCGCCCAGGAAGCTGCCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGCCCTGAATCAGGCCACGATACCAGTCAATATTGAGCAGCTGGGTCATGACATCGTTGG > NC_000913/4151088‑4151557 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |