BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	424,700	C→T	3.0%	A73A (GCG→GCA)	acpH ←	acyl carrier protein (ACP) phosphodiesterase, ACP hydrolyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	424,700	0	C	T	3.0%	470.9 / 2.0	134	A73A (GCG→GCA)	acpH	acyl carrier protein (ACP) phosphodiesterase, ACP hydrolyase
Reads supporting (aligned to +/- strand): ref base C (61/69); new base T (2/2); total (63/71)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.76e-01

Only 100 of 135 total aligned reads displayed.

CGTCTAAATCGTACCAGGAGTCACGCAGGGCATCCAGACGTGGGCGGCGGCTTGCCATGCCGTTTAACACGTTCTGGATGAAATCCATATCGCGATAGCGCACCAGCCACTGCTCTGACCACAAGTAATTGTTCAGATTGATAAAACGTGGCGGTGAGTCCGGCAAAATCGTCATCACTTGCTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCACTACGAAACCACTCCCGTGCTTCGCGGACTTCCGGCAGATTGTCAGTCAATACGTCGATACGTCGATGCATATGAATGCCAGCCAC‑GACGTCGGGCGGAAAACTTTCTTCAGGATTTCCGCGTACGAAATCAGCCAGTAAATTGCCGGAAAGCGAGCTTTCCGCGAGATGGGCTAAATGCAGGTGAGCTAAAAAATTCATCGATTATATTCTATCCAAAAGGGGGTAAAGGTTGCAGGGAGAGCGCCCCGGCACTAGACTACCCGCCTC  >  NC_000913/424417‑424987

cGTCTAAATCGTACCAGGAGTCACGCAGGGCATCCAGACGTGGGCGGCGGCTTGCCATGCCGTTTAACACGTTCTGGATGAAATCCATATCGCGATAGCGCACCAGCCACTGCTCTGACCACAAGTAATTGTTCAGATTGATAAAACGTGGCGGTGAGTCCGGCAAAATCGTCATCACTTGCTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGGGATCCCACATGACATCCAGCGTAATAGGCGCAAc                                                                                                                                                                                                                                                                                             >  1:150820/1‑289 (MQ=255)

cGTCTAAATCGTACCAGGAGTCACGCAGGGCATCCAGACGTGGGCGGCGGCTTGCCATGCCGTTTAACACGTTCTGGATGAAATCCATATCGCGATAGCGCACCAGCAACTGCTCTGATCACAAGTAATTGTTCAGATTGATAAAACGTGTCGGTGAGTCCGGCAAAATCGTCATCACTTGCTCGCGGGCATCACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAAAAAGTGATCCCAAATGACATCCAGCGTAATAGGCGAAAc                                                                                                                                                                                                                                                                                             >  1:730232/1‑289 (MQ=255)

   cTAAATCGTACCAGGAGTCACGCAGGGCATCCAGACGTGGGCGGCGGCTTGCCATGCCGTTTAACACGTTCTGGATGAAATCCATATCGCGATAGCGCACCAGCCACTGCTCTGACCACAAGTAATTGTTCAGATTGATAAAACGTGGCGGTGAGTCCGGCAAAATCGTCATCACTTGCTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGCGCAAcgcg                                                                                                                                                                                                                                                                                          <  2:806842/289‑1 (MQ=255)

            aCCAGGAGTCACGCAGGGCATCCAGACGTGGGCGGCGGCTTGCCATGCCGTTTAACACGTTCTGGATGAAATCCATATCGCGATAGCGCACCAGCCACTGCTCTGACCACAAGTAATTGTTCAGATTGATAAAACGTGGCGGTGAGTCCGGCAAAATCGTCATCACTTGCTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGCGCAACGCGGCGCGttt                                                                                                                                                                                                                                                                                  <  2:525515/288‑1 (MQ=255)

                ggAGTCACGCAGGGCATCCAGACGTGGGCGGCGGCTTGCCATGCCGTTTAACACGTTCTGGATGAAATCCATATCGCGATAGCGCACCAGCCACTGCTCTGACCACAAGTAATTGTTCAGATTGATAAAACGTGGCGGTGAGTCCGGCAAAATCGTCATCACTTGCTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCaac                                                                                                                                                                                                                                                                              >  2:704801/1‑286 (MQ=255)

                      aCGCAGGGCATCCAGACGTGGGCGGCGGCTTGCCATGCCGTTTAACACGTTCTGGATGAAATCCATATCGCGATAGCGCACCAGCCACTGCTCTGACCACAAGTAATTGTTCAGATTGATAAAACGTGGCGGTGAGTCCGGCAAAATCGTCATCACTTGCTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCACTACGAAAc                                                                                                                                                                                                                                                                       <  2:592854/289‑1 (MQ=255)

                                tCCAGACGTGGGCGGCGGCTTGCCATGCCGTTTAACACGTTCTGGATGAAATCCATATCGCGATAGCGCACCAGCCACTGCTCTGACCACAAGTAATTGTTCAGATTGATAAAACGTGGCGGTGAGTCCGGCAAAATCGTCATCACTTGCTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCACTa                                                                                                                                                                                                                                                                             >  2:241697/1‑273 (MQ=255)

                                tCCAGACGTGGGCGGCGGCTTGCCATGCCGTTTAACACGTTCTGGATGAAATCCATATCGCGATAGCGCACCAGCCACTGCTCTGACCACAAGTAATTGTTCAGATTGATAAAACGTGGCGGTGAGTCCGGCAAAATCGTCATCACTTGCTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCACTa                                                                                                                                                                                                                                                                             <  1:241697/273‑1 (MQ=255)

                                  cAGACGTGGGCGGCGGCTTGCCATGCCGTTTAACACGTTCTGGATGAAATCCATATCGCGATAGCGCACCAGCCACTGCTCTGACCACAAGTAATTGTTCAGATTGATAAAACGTGGCGGTGAGTCCGGCAAAATCGTCATCACTTGCTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCACTACGAAACCACTCCCGTGCt                                                                                                                                                                                                                                                           <  1:24658/289‑1 (MQ=255)

                                      cGTGGGCGGCGGCTTGCCATGCCGTTTAACACGTTCTGGATGAAATCCATATCGCGATAGCGCACCAGCCACTGCTCTGACCACAAGTAATTGTTCAGATTGATAAAACGTGGCGGTGAGTCCGGCAAAATCGTCATCACTTGCTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGCACTCCAGCGTAATAGGCGCAACGCGGCCCGTTTCACTAAGAAACCACTCCCGTGcctac                                                                                                                                                                                                                                                        >  1:749110/1‑284 (MQ=255)

                                          ggcggcggcTTGCCATGCCGTTTAACACGTTCTGGATGAAATCCATATCGCGATAGCGCACCAGCCACTGCTCTGACCACAAGTAATTGTTCAGATTGATAAAACGTGGCGGTGAGTCCGGCAAAATCGTCATCACTTGCTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGCGCAACGCGGCGCGGTTCACTACGAAACCACTCCCGTGCTTCGCgg                                                                                                                                                                                                                                                     >  2:547312/1‑287 (MQ=255)

                                               cggcTTGCCATGCCGTTTAACACGTTCTGGATGAAATCCATATCGCGATAGCGCACCAGCCACTGCTCTGACCACAAGTAATTGTTCAGATTGATAAAACGTGGCGGTGAGTCCGGCAAAATCGTCATCACTTGCTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCACTACGAAAc                                                                                                                                                                                                                                                                       >  2:552429/1‑264 (MQ=255)

                                               cggcTTGCCATGCCGTTTAACACGTTCTGGATGAAATCCATATCGCGATAGCGCACCAGCCACTGCTCTGACCACAAGTAATTGTTCAGATTGATAAAACGTGGCGGTGAGTCCGGCAAAATCGTCATCACTTGCTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCACTACGAAAc                                                                                                                                                                                                                                                                       <  1:552429/264‑1 (MQ=255)

                                               cggcTTGCCATGCCGTTTAACACGTTCTGGATGAAATCCATATCGCGATAGCGCACCAGCCACTGCTCTGACCACAAGTAATTGTTCAGATTGATAAAACGTGGCGGTGAGTCCGGCAAAATCGTCATCACTTGCTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCACTACGAAACCACTCCCGTGCTTCGCGGACTTCCg                                                                                                                                                                                                                                              >  2:594943/1‑289 (MQ=255)

                                                        aagCCGTTTAACACGTTCAGGATGAAATCCATAGCGCGATAGCGCACCAGCCACTGCTCTGACCACAAGTAATTGTTCAGATTGATAAAACGTGGCGGTGAGTCCGGCAAAATCGTCATCACTTGCTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCACTACGAAACCACTCCCGTGCTTCGCGGACTTCCGGCAGATTgt                                                                                                                                                                                                                                     <  2:71401/287‑1 (MQ=255)

                                                        aTGCCGTTTAACACGTTCTGGATGAAATCCATATCGCGATAGCGCACCAGCCACTGCTCTGACCACAAGTAATTGTTCAGATTGATAAAACGTGGCGGTGAGTCCGGCAAAATCGTCATCACTTGCTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCACTACGAAACCACTCCCGTGCTTCGCGGACTTCCGGCAGATTgt                                                                                                                                                                                                                                     <  2:740075/289‑1 (MQ=255)

                                                             gTTTAACACGTTCTGGATGAAATCCATATCGCGATAGCGCACCAGCCACTGCTCTGACCACAAGTAATTGTTCAGATTGATAAAACGTGGCGGTGAGTCCGGCAAAATCGTCATCACTTGCTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCACTACGAAACCACTCCCGTGCTTCGCGGACTTCCGGCAGATTgtcagtc                                                                                                                                                                                                                                <  1:169439/289‑1 (MQ=255)

                                                              tttAACACGTTCTGGATGAAATCCATATCGCGATAGCGCACCAGCCACTGCTCTGACCACAAGTAATTGTTCAGATTGATAAAACGTGGCGGTGAGTCCGGCAAAATCGTCATCACTTGCTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTACCTACGAAACCACCCCCGTCCCTCGCGGACTTCCGGCAGATTggccgtcc                                                                                                                                                                                                                               >  1:126120/1‑288 (MQ=255)

                                                                      gTTCTGGATGAAATCCATATCGCGATAGCGCACCAGCCACTGCTCTGACCACAAGTAATTGTTCAGATTGATAAAACGTGGCGGTGAGTCCGGCAAAATCGTCATCACTTGCTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGTGCAACGCGGCGCGTTTCACTACGAAACCACTCCCGTGCTTCGCGGACTTCCGGCAGATTGTCAGTCaa                                                                                                                                                                                                                              >  1:354146/1‑282 (MQ=255)

                                                                      gTTCTGGATGAAATCCATATCGCGATAGCGCACCAGCCACTGCTCTGACCACAAGTAATTGTTCAGATTGATAAAACGTGGCGGTGAGTCCGGCAAAATCGTCATCACTTGCTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGTGCAACGCGGCGCGTTTCACTACGAAACCACTCCCGTGCTTCGCGGACTTCCGGCAGATTGTCAGTCaa                                                                                                                                                                                                                              <  2:354146/282‑1 (MQ=255)

                                                                        tCTGGATGAAATCCATATCGCGATAGCGCACCAGCCACTGCTCTGACCACAAGTAATTGTTCAGATTGATAAAACGTGGCGGTGAGTCCGGCAAAATCGTCATCACTTGCTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCACTACGAAACCACTCCCGTGCTTCGCGGACTTCCGGCAGATTGTCAGTCAatacgtcgat                                                                                                                                                                                                                     >  2:332047/1‑289 (MQ=255)

                                                                        tCTGGATGAAATCCATATCGCGATAGCGCACCAGCCACTGCTCTGACCACAAGTAATTGTTCAGATTGATAAAACGTGGCGGTGAGTCCGGCAAAATCGTCATCACTTGCTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGAACCCACATGACATCCAGCGGAATAGGCGCAACTCGGCGCGGTTCACTACGAAAACACTCCCGTGCTTCGCCGACTTCCGGccgctttgcaagtaaaacctccaa                                                                                                                                                                                                                     >  1:72539/1‑266 (MQ=255)

                                                                             aTGAAATCCATATCGCGATAGCGCACCAGCCACTGCTCTGACCACAAGTAATTGTTCAGATTGATAAAACGTGGCGGTGAGTCCGGCAAAATCGTCATCACTTGCTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCACTACGAAACCACTCCCGTGCTTCGCGGACTTCCGGCAGATTGTCAGTCAatacgtcgatacgt                                                                                                                                                                                                                 <  2:168083/288‑1 (MQ=255)

                                                                               gAAATCCATATCGCGATAGCGCACCAGCCACTGCTCTGACCACAAGTAATTGTTCAGATTGATAAAACGTGGCGGTGAGTCCGGCAAAATCGTCATCACTTGCTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCACTACGAAACCACTCCCGTGCTTCGCGGACTTCCGGCAGATTGTCAGTCAAtacgtcgatacgtcga                                                                                                                                                                                                              <  2:730232/289‑1 (MQ=255)

                                                                                 aaTCCATATCGCGATAGCGCACCAGCCACTGCTCTGACCACAAGTAATTGTTCAGATTGATAAAACGTGGCGGTGAGTCCGGCAAAATCGTCATCACTTGCTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCACTACGAAACCACTCCCGTGCTTCGCGGACTTCCGGCAGATTGTCAGTCAATacgtcgatacgtcgat                                                                                                                                                                                                             >  2:190869/1‑288 (MQ=255)

                                                                                  aTCCATATCGCGATAGCGCACCAGCCACTGCTCTGACCACAAGTAATTGTTCAGATTGATAAAACGTGGCGGTGAGTCCGGCAAAATCGTCATCACTTGCTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCACTACGAAACCACTCCCGTGCTTCGCGGACTTCCGGCAGATTGTCAGTCAATACGTCGATACGTCGATg                                                                                                                                                                                                            >  1:254457/1‑288 (MQ=255)

                                                                                  aTCCATATCGCGATAGCGCACCAGCCACTGCTCTGACCACAAGTAATTGTTCAGATTGATAAAACGTGGCGGTGAGTCCGGCAAAATCGTCATCACTTGCTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCACTACGAAACCACTCCCGTGCTTCGCGGACTTCCGGCAGATTGTCAGTCAATACGTCGATACGTCGATGc                                                                                                                                                                                                           <  1:396048/289‑1 (MQ=255)

                                                                                      atatCGCGATAGCGCACCAGCCACTGCTCTGACCACAAGTAATTGTTCAGATTGATAAAACGTGGCGGTGAGTCCGGCAAAATCGTCATCACTTGCTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCACTACGAAACCACTCCCGTGCTTCGCGGACTTCCGGCAGATTGTCAGTCAATACGTCGATACGTCGATGCatat                                                                                                                                                                                                       >  1:875541/1‑289 (MQ=255)

                                                                                      atatCGCGATAGCGCACCAGCCACTGCTCTGACCACAAGTAATTGTTCAGATTGATAAAACGTGGCGGTGAGTCCGGCAAAATCGTCATCACTTGCTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCATAATAGGCGCAACGCGGCGCGTTTCACTACGAAACCACCCCCGTGCTTCGCGGACTTCCGGCAGCTTGTCAGTCAagacgtcgatacgctcattcattt                                                                                                                                                                                                       >  1:581898/1‑278 (MQ=255)

                                                                                                  cgcACCAGCCACTGCTCTGACCACAAGTAATTGTTCAGATTGATAAAACGTGGCGGTGAGTCCGGCAAAATCGTCATCACTTGCTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGTGCAACGCGGCGCGTTTCACTACGAAACCACTCCCGTGCTTCGCGGACTTCCGGCAGATTGTCAGTCAATACGTCGATACGTCGATGCATATGAATgccagcc                                                                                                                                                                                            >  1:829434/1‑288 (MQ=255)

                                                                                                         gCCACTGCTCTGACCACAAGTAATTGTTCAGATTGATAAAACGTGGCGGTGAGTCCGGCAAAATCGTCATCACTTGCTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCACTACGAAACCACTCCCGTGCTTCGCGGACTTCCGGCAGATTGTCAGTCAATACGTCGATACGTCGATGCATATGAATGCCAGCCAC‑GACGTc                                                                                                                                                                                   >  2:537278/1‑289 (MQ=255)

                                                                                                         gCCACTGCTCTGACCACAAGTAATTGTTCAGATTGATAAAACGTGGCGGTGAGTCCGGCAAAATCGTCATCACTTGCTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCACTACGAAACCACTCCCGTGCTTCGCGGACTTCCGGCAGATTGTCAGTCAATACGTCGATACGTCGATGCATATGAATGCCAGCCAC‑GACGTc                                                                                                                                                                                   >  1:880994/1‑289 (MQ=255)

                                                                                                                        acaAGTAATTGTTCAGATTGATAAAACGTGGCGGTGAGTCCGGCAAAATCGTCATCACTTGCTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCACTACGAAACCACTCCCGTGCTTCGCGGACTTCCGGCAGATTGTCAGTCAATACGTCGATACGTCGATGCATATGAATGCCAGCCAC‑GACGTCGGGCGGAAAACTttc                                                                                                                                                                    <  2:875541/289‑1 (MQ=255)

                                                                                                                          aaGTAATTGTTCAGATTGATAAAACGTGGCGGTGAGTCCGGCAAAATCGTCATCACTTGCTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCACTACGAAACCACTCCCGTGCTTCGCGGACTTCCGGCAGATTGTCAGTCAATACGTCGATACGTCGATGCATATGAATGCCAGCCAC‑GACGTCGGGCGGAAAACTttctt                                                                                                                                                                  >  1:844874/1‑289 (MQ=255)

                                                                                                                               aTTGTTCAGATTGATAAAACGTGGCGGTGAGTCCGGCAAAATCGTCATCACTTGCTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCACTACGAAACCACTCCCGTGCTTCGCGGACTTCCGGCAGATTGTCAGTCAATACGTCGATACGTCGATGCATATGAATGCCAGCCAC‑GACGTCGGGCGGAAAACTTTCTTCAGGa                                                                                                                                                             >  2:250947/1‑289 (MQ=255)

                                                                                                                               aTTGTTCAGATTGATAAAACGTGGCGGTGAGTCCGGCAAAATCGTCATCACTTGCTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCACTACGAAACCACTCCCGTGCTTCGCGGACTTCCGGCAGATTGTCAGTCAATACGTCGATACGTCGATGCATATGAATGCCAGCCAC‑GACGTCGGGCGGAAAACTTTCTTCAGGa                                                                                                                                                             >  2:276909/1‑289 (MQ=255)

                                                                                                                               aTTGTTCAGATTGATAAAACGTGGCGGTGAGTCCGGCAAAATCGTCATCACTTGCTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCACTACGAAACCACTCACGTGCTTCGCGGACTTCCGGCAGATTGTCAGTCAATACGTCGATACGTCCATGCATACGAATGCCAGCCACGGACG‑CGGGCGGGAAAATTTCTTCAGGa                                                                                                                                                             >  1:196431/1‑289 (MQ=255)

                                                                                                                               aTTGTTCAGATTGATAAAACGTGGCGGTGAGTCCGGCAAAATCGTCATCACTTGCTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCACTACGAAACACCTCCCGTGCTTCGCGGACTTCCGGCCGATTGTCAGTCAATACGTCGCTACGTCGATGCATATGAATGACACGCAC‑GACGTCGGCCGGAAAACTTTCTTAAGGa                                                                                                                                                             >  1:588581/1‑289 (MQ=255)

                                                                                                                               aTTGTTCAGATTGATAAAACGTGGCGGTGAGTCCGGCAAAATCGTCATCACTTGCTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGCCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCACTACGAAACCACTCCCGTGCTTCGCGGACTTCCGGCAGATTGTCAGTCAATACGTCGATACGTCGATGCATATGAATGCCAGCCAC‑GACGTCGGGCGGAAAACTTTCTTCAGGa                                                                                                                                                             <  2:363078/289‑1 (MQ=255)

                                                                                                                                  gTTCAGATTGATAAAACGTGGCGGTGAGTCCGGCAAAATCGTCATCACTTGCTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCACTACGAAACCACTCCCGTGCTTCGCGGACTTCCGGCAGATTGTCAGTCAATACGTCGATACGTCGATGCATATGAATGCCAGCCAC‑GACGTCGGGCGGAAAACTTTCTTCAGGAtt                                                                                                                                                           >  2:209830/1‑288 (MQ=255)

                                                                                                                                    tCAGATTGATAAAACGTGGCGGTGAGTCCGGCAAAATCGTCATCACTTGCTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCACTACGAAAc                                                                                                                                                                                                                                                                       >  1:436522/1‑179 (MQ=255)

                                                                                                                                    tCAGATTGATAAAACGTGGCGGTGAGTCCGGCAAAATCGTCATCACTTGCTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCACTACGAAAc                                                                                                                                                                                                                                                                       <  2:436522/179‑1 (MQ=255)

                                                                                                                                    tCAGATTGATAAAACGTGGCGGTGAGTCCGGCAAAATCGTCATCACTTGCTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCACTACGAAACCACTCCCGTGCTTCGCGGACCTCCGGCAGATTGTCAGTCAATACGTCGATACGTCGATGCATATGAATGCCAGCCAC‑GACGTCGTGCGGAAAACTTTCTTCACGATGTc                                                                                                                                                         >  1:214145/1‑288 (MQ=255)

                                                                                                                                     cAGATTGATAAAACGTGGCGGTGAGTCCGGCAAAATCGTCATCACTTGCTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCACTACGAAACCACTCCCGTGCTTCGCGGACTTCCGGCAGa                                                                                                                                                                                                                                         <  2:739332/208‑1 (MQ=255)

                                                                                                                                     cAGATTGATAAAACGTGGCGGTGAGTCCGGCAAAATCGTCATCACTTGCTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCACTACGAAACCACTCCCGTGCTTCGCGGACTTCCGGCAGa                                                                                                                                                                                                                                         >  1:739332/1‑208 (MQ=255)

                                                                                                                                     cAGATTGATAAAACGTGGCGGTGAGTCCGGCAAAATCGTCATCACTTGCTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCACTACGAAACCACTCCCGTGCTTCGCGGACTTCCGGCAGATTGTCAGTCAATACGTCGATACGTCGATGCATATGAATGCCAGCCAC‑GACGTc                                                                                                                                                                                   <  2:127896/261‑1 (MQ=255)

                                                                                                                                     cAGATTGATAAAACGTGGCGGTGAGTCCGGCAAAATCGTCATCACTTGCTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCACTACGAAACCACTCCCGTGCTTCGCGGACTTCCGGCAGATTGTCAGTCAATACGTCGATACGTCGATGCATATGAATGCCAGCCAC‑GACGTc                                                                                                                                                                                   >  1:127896/1‑261 (MQ=255)

                                                                                                                                     cAGATTGATAAAACGTGGCGGTGAGTCCGGCAAAATCGTCATCACTTGCTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCACTACGAAACCACTCCCGTGCTTCGCGGACTTCCGGCAGATTGTCAGTCAATACGTCGATACGTCGATGCATATGAATGCCAGCCAC‑GACGTCGGGCGGAAAACTTTCTTCAGGATTTCcg                                                                                                                                                       <  1:783944/289‑1 (MQ=255)

                                                                                                                                        aTTGATAAAACGTGGCGGTGAGTCCGGCAAAATCGTCATCACTTGCTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCACTACGAAACCACTCCCGTGCTTCGCGGACTTCCGGCAGATTGTCAGTCAatacgtcgatacgtcg                                                                                                                                                                                                               >  2:137914/1‑231 (MQ=255)

                                                                                                                                        aTTGATAAAACGTGGCGGTGAGTCCGGCAAAATCGTCATCACTTGCTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCACTACGAAACCACTCCCGTGCTTCGCGGACTTCCGGCAGATTGTCAGTCAATACGTCGATACGTCGATGCATATGAATGCCAGCCAC‑GACGTCGGGCGGAAAACTTTCTTCAGGCTTTCCGCGt                                                                                                                                                    >  2:858009/1‑289 (MQ=255)

                                                                                                                                        aTTGATAAAACGTGGCGGTGAGTCCGGCAAAATCGTCATCACTTGCTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCACTACGAAACCACTCCCGTGCTTCGCGGACTTCCGGCAGATTGTCAGTCAATACGTCGATACGTCGATGCATATGAATGCCAGCCAC‑GACGTCGGGCGGAAAACTTTCTTCAGGATTTCCGCGt                                                                                                                                                    >  1:13087/1‑289 (MQ=255)

                                                                                                                                        aTTGATAAAACGTGGCGGTGAGTCCGGCAAAATCGTCATCACTTGCTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCACTACGAAACACCTCCCGTGCTTCGCGGACTTCCGGCAGATTGTCAGTCAATACGTCGATACGTCGATGCATATGAATGCCAGCCCC‑GACGTCGGCGGGAAAACTTTCTTCAGGGTTTCCGCGt                                                                                                                                                    >  1:320975/1‑289 (MQ=255)

                                                                                                                                         ttGATAAAACGTGGCGGTGAGTCCGGCAAAATCGTCATCACTTGCTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCACTACGAAACCACTCCCGTGCTTCGCGGACTTCCGGCAGATTGTCAGTCAATACGTCGATACGTCGATGCATATGAATGCCAGCCAC‑GACGTCGGGCGGAAAACTTTCTTCAGGATTTCCGCGTa                                                                                                                                                   <  2:39776/289‑1 (MQ=255)

                                                                                                                                         ttGATAAAACGTGGCGGTGAGTCCGGCAAAATCGTCATCACTTGCTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCACTACGAAACCACTCCCGTGCTTCGCGGACTTCCGGCAGATTGTCAGTCAATACGTCGATACGTCGATGCATATGAATGCCAGCCAC‑GACGTCGGGCGGAAAACTTTCTTCAGGATTTCCGCGTa                                                                                                                                                   <  2:320975/289‑1 (MQ=255)

                                                                                                                                               aaaCGTGGCGGTGAGTCCGGCAAAATCGTCATCACTTGCTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCACTACGAAACCACTCCCGTGCTTCGCGGACTTCCGGCAGATTGTCAGTCAATACGTCGATACGTCGATGCATATGAATGCCAGCCAC‑GACGTCGGGCGGAAAACTTTCTTCAGGATTTCCGCGTACGAAAt                                                                                                                                             <  1:537278/289‑1 (MQ=255)

                                                                                                                                                      gCGGTGAGTCCGGCAAAATCGTCATCACTTGCTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCACTACGAAACCACTCCCGTGCTTCGCGGACTTCCGGCAGATTGTCAGTCAATACGTCGATACGTCGATGCATATGAATGCCAGCCAC‑GACGTCGGGCGGAAAACTTTCTTCAGGATTTCCGCGTACGAAATCAGCCAg                                                                                                                                      >  1:191809/1‑289 (MQ=255)

                                                                                                                                                      gCGGTGAGTCCGGCAAAATCGTCATCACTTGCTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCACTACGAAACCACTCCCGTGCTTCGCGGACTTCCGGCAGATTGTCAGTCAATACGTCGATACGTCGATGCATATGAATGCCAGCCAC‑GACGTCGGGCGGAAAACTTTCTTCAGGATTTCCGCGTACGAAATCAGCCAg                                                                                                                                      >  2:779455/1‑289 (MQ=255)

                                                                                                                                                      gCGGTGAGTCCGGCAAAATCGTCATCACTTGCTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCACTACGAAACCACTCCCGTGCTTCGCGGACTTCCGGCAGATTGTCAGTCAATACGTCGATACGTCGATGCATATGAATGCCAGCCAC‑GACGTCGGGCGGAAAACTTTCTTCAGGATTTCCGCGTACGAAATCAGCCAg                                                                                                                                      >  2:810828/1‑289 (MQ=255)

                                                                                                                                                                   cAAAATCGTCATCACTTGCTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCACTACGAAACCACTCCCGTGCTTCGCGGACTTCCGGCAGATTGTCAGTCAATACGTCGATACGTCGATGCATATGAATGCCAGCCAC‑GACGTCGGGCGGAAAACTTTCTTCAGGATTTCCGCGTACGAAATCAGCCAGTAAATTGCCGGaa                                                                                                                         >  1:304971/1‑289 (MQ=255)

                                                                                                                                                                            catcaCTTGCTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCACTACGAAACCACTCCCGTGCTTCGCGGACTTCCGGCAGATTGTCAGTCAATACGTCGATACGTCGATGCATATGAATGCCAGCCAC‑GACGTCGGGCGGAAAACTTTCTTCAGGATTTCCGCGTACGAAATCAGCCAGTAAATTGCCGGAAAGCGAGCtt                                                                                                                >  1:629006/1‑289 (MQ=255)

                                                                                                                                                                            catcaCTTGCTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCACTACGAAACCACTCCCGTGCTTCGCGGACTTCCGCCAGATTGTCAGTCAATACGTCGATACGTCGCTGCATATGAATGCCAGCCAC‑GACGTCGGTCGGGAAACTTTCGTCAAGATTTTCGCCTACGAAATCAGCCAGGAAATTGCCGGAAAggcaactt                                                                                                                >  1:366559/1‑282 (MQ=255)

                                                                                                                                                                                     cTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCACTACGAAACCACTCCCGTGCTTCGCGGACTTCCGGCAGATTGTCAGTCAATACGTCGATACGTCGATGCATATGAATGCCAGCCAC‑GACGTCGGGCGGAAAACTTTCTTCAGGATTTCCGCGTACGAAATCAGCCAGTAAATTGCCGGAAAGCGAGCTTTCCGCgaga                                                                                                       >  2:846662/1‑289 (MQ=255)

                                                                                                                                                                                      tCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCACTACGAAACCACTCCCGTGCTTCGCGGACTTCCGGCAGATTGTCAGTCAATACGTCGATACGTCGATGCATATGAATGCCAGCCAC‑GACGTCGGGCGGAAAACTTTCTTCAGGATTTCCGCGTACGAAATCAGCCAGTAAATTGCCGGAAAGCGAGCTTTCCGCgaga                                                                                                       <  2:719071/288‑1 (MQ=255)

                                                                                                                                                                                          gggCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCACTACGAAACCACTCCCGTGCTTCGCGGACTTCCGGCAGATTGTCAGTCAATACGTCGATACGTCGATGCATATGAATGCCAGCCAC‑GACGTCGGGCGGAAAACTTTCTTCAGGATTTCCGCGTACGAAATCAGCCAGTAAATTGCCGGAAAGCGAGCTTTCCGCGAGATggg                                                                                                   <  2:675896/288‑1 (MQ=255)

                                                                                                                                                                                            gCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCACTACGAAACCACTCCCGTGCTTCGCGGACTTCCGGCAGATTGTCAGTCAATACGTCGATACGTCGATGCATATGAATGCCAGCCAC‑GACGTCGGGCGGAAAACTTTCTTCAGGATTTCCGCGTACGAAATCAGCCAGTAAATTGCCGGAAAGCGAGCTTTCCGCGAGATGGGCt                                                                                                 <  1:697314/288‑1 (MQ=255)

                                                                                                                                                                                            gCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCACTACGAAACCACTCCCGTGCTTCGCGGACTTCCGGCAGATTGTCAGTCAATACGTCGATACGTCGATGCATATGAATGCCAGCCAC‑GACGTCGGGCGGAAAACTTTCTTCAGGATTTCCGCGTACGAAATCAGCCAGTAAATTGCCGGAAAGCGAGCTTTCCGCGAGATGGGCt                                                                                                 <  2:304971/288‑1 (MQ=255)

                                                                                                                                                                                                      cAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCACTACGAAACCACTCCCGTGCTTCGCGGACTTCCGGCAGATTGTCAGTCAatacgtcga                                                                                                                                                                                                                      <  1:798060/162‑1 (MQ=255)

                                                                                                                                                                                                          ttCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCACTACGAAACCACTCCCGTGCTTCGCGGACTTCCGGCAGATTGTCAGTCAATACGTCGATACGTCGATGCATATGAATGCCAGCCAC‑GACGTCGGGCGGAAAACTTTCTTCAGGATTTCCGCGTACGAAATCAGCCAGTAAATTGCCGGAAAGCGAGCTTTCCGCGAGATGGGCTAAATGCAGGTGAGCt                                                                                  >  1:202652/1‑289 (MQ=255)

                                                                                                                                                                                                            ccTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCACTACGAAACCACTCCCGTGCTTCGCGGACTTCCGGCAGATTGTCAGTCAATACGTCGATACGTCGATGCATATGAATGCCAGCCAC‑GACGTCGGGCGGAAAACTTTCTTCAGGATTTCCGCGTACGAAATCAGCCAGTAAATTGCCGGAAAGCGAGCTTTCCGCgag                                                                                                        >  1:302816/1‑265 (MQ=255)

                                                                                                                                                                                                            ccTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCACTACGAAACCACTCCCGTGCTTCGCGGACTTCCGGCAGATTGTCAGTCAATACGTCGATACGTCGATGCATATGAATGCCAGCCAC‑GACGTCGGGCGGAAAACTTTCTTCAGGATTTCCGCGTACGAAATCAGCCAGTAAATTGCCGGAAAGCGAGCTTTCCGCgag                                                                                                        <  2:302816/265‑1 (MQ=255)

                                                                                                                                                                                                               gTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCACTACGAAACCACTCCCGTGCTTCGCGGACTTCCGGCAGATTGTCAGTCAATACGTCGATACGTCGATGCATATGAATGCCAGCCAC‑GACGTCGGGCGGAAAACTTTCTTCAGGATTTCCGCGTACGAAATCAGCCAGTAAATTGCCGGAAAGCGAGCTTTCCGCGAGATGGGCTAAATGCAGGTGAGCTaaaaa                                                                             >  1:869946/1‑289 (MQ=255)

                                                                                                                                                                                                                tAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCACTACGAAACCACTCCCGTGCTTCGCGGACTTCCGGCAGATTGTCAGTCAATACGTCGATACGTCGATGCATATGAATGCCAGCCAC‑GACGTCGGGCGGAAAACTTTCTTCAGGATTTCCGCGTACGAAATCAGCCAGTAAATTGCCGGAAAGCGAGCTTTCCGCGAGATGGGCTAAATGCAGGTGAGCTaaaaaa                                                                            >  2:261505/1‑289 (MQ=255)

                                                                                                                                                                                                                      aaaGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCACTACGAAACCACTCCCGTGCTTCGCGGACTTCCGGCAGATTGTCAGTCAATACGTCGATACGTCGATGCATATGAATGCCAGCCAC‑GACGTCGGGCGGAAAACTTTCTTCAGGATTTCCGCGTACGAAATCAGCCAGTAAATTGCCGGAAAGCGAGCTTTCCGCGAGATGGGCTAAATGCAGGTGAGCTAAAAAATTCAt                                                                       <  2:191809/288‑1 (MQ=255)

                                                                                                                                                                                                                      aaaGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCACTACGAAACCACTCCCGTGCTTCGCGGACTTCCGGCAGATTGTCAGTCAATACGTCGATACGTCGATGCATATGAATGCCAGCCAC‑GACGTCGGGCGGAAAACTTTCTTCAGGATTTCCGCGTACGAAATCAGCCAGTAAATTGCCGGAAAGCGAGCTTTCCGCGAGATGGGCTAAATGCAGGTGAGCTAAAAAATTCAt                                                                       <  1:261505/288‑1 (MQ=255)

                                                                                                                                                                                                                      aaaGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCACTACGAAACCACTCCCGTGCTTCGCGGACTTCCGGCAGATTGTCAGTCAATACGTCGATACGTCGATGCATATGAATGCCAGCCAC‑GACGTCGGGCGGAAAACTTTCTTCAGGATTTCCGCGTACGAAATCAGCCAGTAAATTGCCGGAAAGCGAGCTTTCCGCGAGATGGGCTAAATGCAGGTGAGCTAAAAAATTCAt                                                                       <  2:581898/288‑1 (MQ=255)

                                                                                                                                                                                                                      aaaGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCACTACGAAACCACTCCCGTGCTTCGCGGACTTCCGGCAGATTGTCAGTCAATACGTCGATACGTCGATGCATATGAATGCCAGCCAC‑GACGTCGGGCGGAAAACTTTCTTCAGGATTTCCGCGTACGAAATCAGCCAGTAAATTGCCGGAAAGCGAGCTTTCCGCGAGATGGGCTAAATGCAGGTGAGCTAAAAAATTCAt                                                                       <  2:214145/288‑1 (MQ=255)

                                                                                                                                                                                                                      aaaGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCACTACGAAACCACTCCCGTGCTTCGCGGACTTCCGGCAGATTGTCAGTCAATACGTCGATACGTCGATGCATATGAATGCCAGCCAC‑GACGTCGGGCGGAAAACTTTCTTCAGGATTTCCGCGTACGAAATCAGCCAGTAAATTGCCGGAAAGCGAGCTTTCCGCGAGATGGGCTAAATGCAGGTGAGCTAAAAAATTCAt                                                                       <  2:629006/288‑1 (MQ=255)

                                                                                                                                                                                                                      aaaGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCACTACGAAACCACTCCCGTGCTTCGCGGACTTCCGGCAGATTGTCAGTCAATACGTCGATACGTCGATGCATATGAATGCCAGCCAC‑GACGTCGGGCGGAAAACTTTCTTCAGGATTTCCGCGTACGAAATCAGCCAGTAAATTGCCGGAAAGCGAGCTTTCCGCGAGATGGGCTAAATGCAGGTGAGCTAAAAAATTCAt                                                                       <  2:13087/288‑1 (MQ=255)

                                                                                                                                                                                                                      aaaGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCACTACGAAACCACTCCCGTGCTGCGCGGACTTCCGGCAGATTGTCAGTCAATACGTCGATACGTCGGTGCATATGAATGCCAGCCAC‑GACGTCGGGCGGAAAACTTTCTTCAGGATTTCCGCGTACGAAATCAGCCAGTAAATTGCCGGAAAGCGAGCTTTCCGCGAGATGGGCTAAATGCAGGTGAGCTAAAAAAGTCAt                                                                       <  2:580804/288‑1 (MQ=255)

                                                                                                                                                                                                                        aGTCCGGCGCCAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCACTACGAAACCACTCCCGTGCTTCGCGGACTTCCGGCAGATTGTCAGTCAATACGTCGATACGTCGATGCATATGAATGCCAGCCAC‑GACGTCGGGCGGAAAACTTTCTTCAGGATTTCCGCGTACGAAATCAGCCAGTAAATTGCCGGAAAGCGAGCTTTCCGCGAGATGGGCTAAATGCAGGTGAGCTAAAAAATTCATCGa                                                                    <  2:588031/289‑1 (MQ=255)

                                                                                                                                                                                                                             ggCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCACTACGAAACCACTCCCGTGCTTCGCGGACTTCCGGCAGATTGTCAGTCAATACGTCGATACGTCGATGCATATGAATGCCAGCCAC‑GACGTCGGGCGGAAAACTTTCTTCAGGATTTCCGCGTACGAAATCAGCCAGTAAATTGCCGGAAAGCGAGCTTTCCGCGAGATGGGCTAAATGCAGGTGAGCTAAAAAATTCATCGATtata                                                               <  1:756678/289‑1 (MQ=255)

                                                                                                                                                                                                                              gCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCACTACGAAACCACTCCCGTGCTTCGCGGACTTCCGGCAGATTGTCAGTCAATACGTCGATACGTAGATGCATATGAATGGCAGCCAC‑GACGTCGGGCGGAAAACTTTCTTCAGGATTCCCGCGTACGAAATCAGCCAGTAAATTGCCGGAAAGCGAGCTTTCCGCGAGATGGGCTAAATGCAGGTGAGCTAAAAAATTTATCGGTTatat                                                              >  2:773249/1‑289 (MQ=255)

                                                                                                                                                                                                                                         gACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCACTACGAAACCACTCCCGTGCTTCGCGGACTTCCGGCAGATTGTCAGTCAATACGTCGATACGTCGATGCATATGAATGCCAGCCAC‑GACGTCGGGCGGAAAACTTTCTTCAGGATTTCCGCGTACGAAATCAGCCAGTAAATTGCCGGAAAGCGAGCTTTCCGCGAGATGGGCTAAATGCAGGTGAGCTAAAAAATTCATCGATTATATTCTATCCaaa                                                    <  2:254457/288‑1 (MQ=255)

                                                                                                                                                                                                                                          aCCAGTGGCGGGAAAGAGAGTGATCCCACATGACATCCAGCGTAATGGGCGCAACGCGGCGCGTTTCACTACGAAACCAGTCCCGTGCTTCGCGGACTTCCGGCAGATTGTCAGTCAATACGTCGATACGTCGATGCATATGAATGCCAGCCAC‑GACGTCGGGCGGAAAACTTTCTTCAGGATTTCCGCGTACGAAATCAGCCAGTAAATTGCCGGAAAGCGAGCTTTCCGCGAGATGGGCTAAATGCAGGTGAGCTAAAAAATTCATCGATTATATTCTATCCaaaa                                                   <  1:164853/288‑1 (MQ=255)

                                                                                                                                                                                                                                          aCCAGGGGCGGGAAAGAAAGGGAACCCACATGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCACTACGAAACCACTCCCGTGCTTCGCGGACTTCCGGCAGATTGTCAGTCAATACGTCGATACGTCGATGCATATGAATGCCAGCCAC‑GACGTCGGGCGGAAAACTTTCTTCAGGATTTCCGCGTACGAAATCAGCCAGTAAATTGCCGGAAAGCGAGCTTTCCGCGAGATGGGCTAAATGCAGGTGAGCTAAAAAATTCATCGATTATATTCTATCCaaaa                                                   <  1:594943/288‑1 (MQ=255)

                                                                                                                                                                                                                                               tGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCACTACGAAACCACTCCCGTGCTTCGCGGACTTCCGGCAGATTGTCAGTCAATACGTCGATACGTCGATGCATATGAATGCCAGCCAC‑GACGTCGGGCGGAAAACTTTCTTCAGGATTTCCGCGTACGAAATCAGCCAGTAAATTGCCGGAAAGCGAGCTTTCCGCGAGATGGGCTAAATGCAGGTGAGCTAAAAAATTCATCGATTATATTCTATCCAAAAGGGGGt                                             >  2:522787/1‑289 (MQ=255)

                                                                                                                                                                                                                                                  cGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGTGCAACGCGGCGCGTTTCACTACGAAACCACTCCCGTGCTTCGCGGACTTCCGGCAGATTGTCAGTCAATACGTCGATACGTCGATGCATATGAATGCCAGCCAC‑GACGTCGGGCGGAAAACTTTCTTCAGGATTTCCGCGTACGAAATCAGCCAGTAAATTGCCGGAAAGCGAGCTTTCCGCGAGATGGGCTAAATGCAGGTGAGCTAAAAAATTCATCGATTATATTCTATCCAAAAGGGGGTaa                                           <  2:829434/288‑1 (MQ=255)

                                                                                                                                                                                                                                                     gaaagaaaGTGATCCCACATGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCACTACGAAACCACTCCCGTGCTTCGCGGACTTCCGGCAGATTGTCAGTCAATACGTCGATACGTCGATGCATATGAATGCCAGCCAC‑GACGTCGGGCGGAAAACTTTCTTCAGGATTTCCGCGTACGAAATCAGCCAGTAAATTGCCGGAAAGCGAGCTTTCCGCGAGATGGGCt                                                                                                 >  1:721412/1‑231 (MQ=255)

                                                                                                                                                                                                                                                       aagaaagTGATCCCACATGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCACTACGAAACCACTCCCGTGCTTCGCGGACTTCCGGCAGATTGTCAGTCAATACGTCGATACGTCGATGCATATGAATGCCAGCCAC‑GACGTCGGGCGGAAAACTTTCTTCAGGATTTCCGCGTACGAAATCAGCCAGTAAATTGCCGGAAAGCGAGCTTTCCGCGAGATGGGCTAAATGCAGGTGAGCTAAAAAATTCATCGATTATATTCTATCCAAAAGGGGGTAAAGGtt                                      >  2:644619/1‑288 (MQ=255)

                                                                                                                                                                                                                                                         gaaagTGATCCCACATGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCACTACGAAACCACTCCCGTGCTTCGCGGACTTCCGGCAGATTGTCAGTCAATACGTCGATACGTCGATGCATATGAATGCCAGCCAC‑GACGTCGGGCGGAAAACTTTCTTCAGGATTTCCGCGTACGAAATCAGCCAGTAAATTGCCGGAAAGCGAGCTTTCCGCGAGATGGGCTAAATGCAGGTGAGCTAAAAAATTCATCGATTATAtt                                                             >  2:393181/1‑263 (MQ=255)

                                                                                                                                                                                                                                                         gaaagTGATCCCACATGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCACTACGAAACCACTCCCGTGCTTCGCGGACTTCCGGCAGATTGTCAGTCAATACGTCGATACGTCGATGCATATGAATGCCAGCCAC‑GACGTCGGGCGGAAAACTTTCTTCAGGATTTCCGCGTACGAAATCAGCCAGTAAATTGCCGGAAAGCGAGCTTTCCGCGAGATGGGCTAAATGCAGGTGAGCTAAAAAATTCATCGATTATAtt                                                             <  1:393181/263‑1 (MQ=255)

                                                                                                                                                                                                                                                                      caTGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCGCTACGAAACCACTCCCGTGCTTCGCGGACTTCCGGCAGATTGTCAGTCAATACGTCGATACGTCGATGCATATGAATGCCAGCCAC‑GACGTCGGGCGGAAAACTTTCTTCAGGATTTCCGCGTACGAAATCAGCCAGTAAATTGCCGGAAAGCGAGCTTTCCGCGAGATGGGCTAAATGCAGGTGAGCTAAAAAATTCATCGATTATATTCTATCCAAAAGGGGGTAAAGGTTGCAGGGAGAGCGcccc                      >  1:25600/1‑289 (MQ=255)

                                                                                                                                                                                                                                                                          aCATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCACTACGAAACCACTCCCGTGCTTCGCGGACTTCCGGCAGATTGTCAGTCAATACGTCGATACGTCGATGCATATGAATGCCAGCCAC‑GACGTCGGGCGGAAAACTTTCTTCAGGATTTCCGCGTACGAAATCAGCCAGTAAATTGCCGGAAAGCGAGCTTTCCGCGAGATGGGCTAAATGCAGGTGAGCTAAAAAATTCATCGATTATATTCTATCCAAAAGGGGGTAAAGGTTGCAGGGAGAGCGCCCCGGc                   >  2:769633/1‑288 (MQ=255)

                                                                                                                                                                                                                                                                            aTCCAGCGTAATAGGCGCAACGCGGCGCGTTTCACTACGAAACCACTCCCGTGCTTCGCGGACTTCCGGCAGATTGTCAGTCAATACGTCGATACGTCGATGCATATGAATGCCAGCCAC‑GACGTCGGGCGGAAAACTTTCTTCAGGATTTCCGCGTACGAAATCAGCCAGTAAATTGCCGGAAAGCGAGCTTTCCGCGAGATGGGCTAAATGCAGGTGAGCTAAAAAATTCATCGATTATATTCTATCCaaaa                                                   >  1:513375/1‑254 (MQ=255)

                                                                                                                                                                                                                                                                            aTCCAGCGTAATAGGCGCAACGCGGCGCGTTTCACTACGAAACCACTCCCGTGCTTCGCGGACTTCCGGCAGATTGTCAGTCAATACGTCGATACGTCGATGCATATGAATGCCAGCCAC‑GACGTCGGGCGGAAAACTTTCTTCAGGATTTCCGCGTACGAAATCAGCCAGTAAATTGCCGGAAAGCGAGCTTTCCGCGAGATGGGCTAAATGCAGGTGAGCTAAAAAATTCATCGATTATATTCTATCCaaaa                                                   <  2:513375/254‑1 (MQ=255)

                                                                                                                                                                                                                                                                                 gCGTAATAGGCGCAACGCGGCGCGTTTCACTACGAAACCACTCCCGTGCTTCGCGGACTTCCGGCAGATCGTCAGTCAATACGTCGATACGTCGATGCCTATGAATGCCAGCCAC‑CACGTCTGGCGGAACACTTTCTTCAGGATTTCCGCGTACGAAATCAGCCAGTACATTGCCGGAACGAGCGCTTTCCGCGGGATGGGCCAAATGCAGGCGAGCTAAAAACATCACCGATTACATTCTAACCAACAGGGGGTAAAGGTGGCAgggtagacctcccggcgctacact           >  1:671067/1‑268 (MQ=255)

                                                                                                                                                                                                                                                                                     aaTAGGCGCAACGCGGCGCGTTTCACTACGAAACCACTCCCGTGCTTCGCGGACTTCCGGCAGATTGTCAGTCAATACGTCGATACGTCGATGCATATGAATGCCAGCCAC‑GACGTCGGGCGGAAAACTTTCTTCAGGATTTCCGCGTACGAAATCAGCCAGTAAATTGCCGGAAAGCGAGCTTTCCGCGAGATGGGCTAAATGCAGGTGAGCTAAAAAATTCATCGATTATATTCTATCCAAAAGGGGGTAAAGGTTGCAGGGAGAGCGCCCCGGCACTAGACTAcc        <  2:869946/288‑1 (MQ=255)

                                                                                                                                                                                                                                                                                       tAGGCGCAACGCGGCGCGTTTCACTACGAAACCACTCCCGTGCTTCGCGGACTTCCGGCAGATTGTCAGTCAATACGTCGATACGTCGATGCATATGAATGCCAGCCAC‑GACGTCGGGCGGAAAACTTTCTTCAGGATTTCCGCGTACGAAATCAGCCAGTAAATTGCCGGAAAGCGAGCTTTCCGCGAGAt                                                                                                      <  1:822453/192‑1 (MQ=255)

                                                                                                                                                                                                                                                                                       tAGGCGCAACGCGGCGCGTTTCACTACGAAACCACTCCCGTGCTTCGCGGACTTCCGGCAGATTGTCAGTCAATACGTCGATACGTCGATGCATATGAATGCCAGCCAC‑GACGTCGGGCGGAAAACTTTCTTCAGGATTTCCGCGTACGAAATCAGCCAGTAAATTGCCGGAAAGCGAGCTTTCCGCGAGAt                                                                                                      >  2:822453/1‑192 (MQ=255)

                                                                                                                                                                                                                                                                                           cgcAACGCGGCGCGTTTCACTACGAAACCACTCCCGTGCTTCGCGGACTTCCGGCAGATTGTCAGTCAATACGTCGATACGTCGATGCATATGAATGCCAGCCAC‑GACGTCGGGCGGAAAACTTTCTTCAGGATTTCCGCGTACGAAATCAGCCAGTAAATTGCCGGAAAGCGAGCTTTCCGCGAGATGGGCTAAATGCAGGTGAGCTAAAAAATTCATCGATTATATTCTATCCAAAAGGGGGTAAAGGTTGCAGGGAGAGCGCCCCGGCACTAGACTACCCGCctc  <  2:202652/288‑1 (MQ=255)

CGTCTAAATCGTACCAGGAGTCACGCAGGGCATCCAGACGTGGGCGGCGGCTTGCCATGCCGTTTAACACGTTCTGGATGAAATCCATATCGCGATAGCGCACCAGCCACTGCTCTGACCACAAGTAATTGTTCAGATTGATAAAACGTGGCGGTGAGTCCGGCAAAATCGTCATCACTTGCTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGACATCCAGCGTAATAGGCGCAACGCGGCGCGTTTCACTACGAAACCACTCCCGTGCTTCGCGGACTTCCGGCAGATTGTCAGTCAATACGTCGATACGTCGATGCATATGAATGCCAGCCAC‑GACGTCGGGCGGAAAACTTTCTTCAGGATTTCCGCGTACGAAATCAGCCAGTAAATTGCCGGAAAGCGAGCTTTCCGCGAGATGGGCTAAATGCAGGTGAGCTAAAAAATTCATCGATTATATTCTATCCAAAAGGGGGTAAAGGTTGCAGGGAGAGCGCCCCGGCACTAGACTACCCGCCTC  >  NC_000913/424417‑424987

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`