Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,708,854	C→G	10.2%	Q646E (CAA→GAA)	rsxC →	SoxR iron‑sulfur cluster reduction factor component, putative membrane‑associated NADH oxidoreductase of electron transport complex

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,708,854	0	C	G	10.2%	195.4 / 9.2	82	Q646E (CAA→GAA)	rsxC	SoxR iron‑sulfur cluster reduction factor component, putative membrane‑associated NADH oxidoreductase of electron transport complex
Reads supporting (aligned to +/- strand):  ref base C (36/32);  new base G (5/5);  total (43/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAAC‑CAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAG‑CACGCAA‑GCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACA‑GGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTTGCCGCGGCTATTGCCCGCGCTCAGGCCAAAAAAGCCGCCCAGCAGAAGGTTGTAAACGAGGACTA  >  NC_000913/1708572‑1709140                                                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                                                                gCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAAC‑CAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAG‑CACGCAA‑GCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACA‑gg                                                                                                                                                                                                                                                                                          <  2:736555/289‑1 (MQ=255)          cGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAAC‑CAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCCGAAGAACCGGTCGATCCGCGCCAAGCGGCCGTCGCAGCGGCTATTGCCCGGGCCAAAG‑CGCGCAA‑GCCGGAACAGCAACAGGCTAATGACGAGCCCGAAGAACA‑GGTAGATcct                                                                                                                                                                                                                                                                                  >  1:494437/1‑287 (MQ=14)          cGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAAC‑CAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAG‑CACGCAA‑GCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACA‑GGTCGATCcgc                                                                                                                                                                                                                                                                                 >  2:581763/1‑289 (MQ=255)          cGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAAC‑CAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAG‑CACGCAA‑GCTGGAACAGCAACAGGCTAATGCCGAGCCAGAAAAACA‑GGTCGATCcgc                                                                                                                                                                                                                                                                                 >  2:133941/1‑289 (MQ=255)           gTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAAC‑CAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAG‑CACGCAA‑GCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACCACA‑GGTCGATcccc                                                                                                                                                                                                                                                                                 >  2:428095/1‑286 (MQ=255)                                      cccGCTCTAAAGCGCCGAAGCTGGTGCTGCCAAGGGCTAACGTGGAAC‑CAGAACAACAGGCCGATCCGCGCAAAGCCGCCGTTGAAGCCGCTATCGCCACTGCCAAAGCGCGCAAGCTGGAACAGCAACGGGCTAATGCGGAATCAGAGCAACAGGTCGATCCGCGCAAAGCCGCCGTTGAAGCCGCTATGGCCCGCGCCAAAG‑CACGCAA‑GCTGGAACAGCAACAGGCTAATGCCGAACCAGAACAACA‑GGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGc                                                                                                                                                                                                                                                     <  1:133941/289‑1 (MQ=14)                                       cgcggcaaaaaCGGGCAAGCAGGAAGAGCACCAGGCGAATGCGGACC‑CAGAAGACCAGGACGATCAACGAAAAGCTGCCGTCGAAGCCGCTATTGCCCGGGCCAAAGCGCGCAAGCAGGAACAGCAACAGGCTAATGCGGAACCAGAAGACCGGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAG‑CACGCAA‑GCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACA‑GGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGcc                                                                                                                                                                                                                                                    <  1:799880/282‑1 (MQ=14)                                          cgcCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAAC‑CAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGGACCAGAAGAAAAGGTCGCTCCGCGCAAAGCGGCCGTCGACGCCGCTATTGCCCTGGCCAAAG‑CACGCAA‑CCTGGAACAGCAACAGACAAATGCGGTACCAGAACAACA‑GGTCGACACGCGCCAAGCCGCCGTCGACCCCGCTATTGgccta                                                                                                                                                                                                                                                 >  1:871143/1‑287 (MQ=14)                                             acaagaaagaggtcgggatagacAACAGGCTAATG‑GGAACGCAGAGAGGCAGGTCGAACGACGTACAGCCACCGTCGACGCCGGTATTGGATGTGCCAACGCGCGCAAACGGGAACAGCAACCTGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAATCTGTCGTCGAAGCGGCTATTACACGTGCCAAAG‑CACGCAA‑GCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACA‑GGTTGATCCGCGCAAAGCGGCAGTTGCCGCGGCTATTGCCCgcgct                                                                                                                                                                                                                                              <  1:749037/267‑2 (MQ=17)                                               aaGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAAC‑CAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAG‑CACCCAA‑GCCGGAACCGCAAAAGGATAATGCCGAACCAGGAGAACA‑GGTCGATCCGCGCAAAGCCGCCGCCGACGccaccactcaccttcccta                                                                                                                                                                                                                                            >  1:436405/1‑272 (MQ=14)                                                    cgcAAGCTGGAACAGCAACAGGCTAATGCGGAAC‑CAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAG‑CACGCAA‑ACCGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACA‑GGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAgcgc                                                                                                                                                                                                                                       >  2:605800/1‑289 (MQ=255)                                                            ggAACAGCTACCAGCTAGAGCGGAAC‑CAGAACAGCGGGACGAGCGGCGGAAAGCCGCCGTGGAGGCAGCTATTACCCGCGCAAAGGCGGGCAAGCTGGAACAGCAACAGGCTAATGCGGAAGCAGAAGAACAGGTCGATCGGCGCAAAACCGCCGTCGAAGCAGCTATTGCCCGTGCCAAAG‑CGCGCAA‑GCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACA‑GGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACg                                                                                                                                                                                                                                <  1:346175/288‑1 (MQ=14)                                                                   cAACAGGCTAATGCGGAAC‑CAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCTGCCGTCGAAGCAGCTATTGCCCGTGCCAAAG‑CACGCAA‑GCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACA‑GGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGc                                                                                                                                                                                                                        >  2:749037/1‑289 (MQ=255)                                                                   cAACAGGCTAATGCGGAAC‑CAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAG‑CACGCAA‑GCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACA‑GGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGATCCAAAGCGCGCAAACGGGAACAGc                                                                                                                                                                                                                        >  2:684685/1‑289 (MQ=255)                                                                   cAACAGGCTAATGCGGAAC‑CAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAG‑CACGCAA‑GCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACA‑GGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCCCGCAAACGGGAACAGc                                                                                                                                                                                                                        >  2:474892/1‑289 (MQ=255)                                                                                  ggaacaaaaccacaaGGTAGATCCGCGCAAAGCTGCCGTCGAAGACGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAAAAGAACAGGCCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAG‑CACGCAA‑GCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACA‑GGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGc                                                                                                                                                                                                           <  1:428095/274‑1 (MQ=17)                                                                                   aaC‑CAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGCAGCCGCTATTGCCCGTGCCCAAG‑CCCGCAA‑GCTCGCACAGCCACAGGCTAATGCCGAACCAGAACCACA‑GGTCCATACGCGCAACGCAGCCGTCGCCGCCGCTATTGCCCCAGCCCAACCCCGCAACCTGGCACAGCACCCGGCCAAtcccgc                                                                                                                                                                                                        >  1:640457/1‑284 (MQ=14)                                                                                   aaC‑CAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAG‑CACGCAA‑GCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACA‑GGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCCCAAACGGGAACAGCAACCGGCTAATGCGGa                                                                                                                                                                                                        >  2:557934/1‑289 (MQ=255)                                                                                             aacaGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAG‑CACGCAA‑GCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACA‑GGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCagaaga                                                                                                                                                                                               <  2:245530/289‑1 (MQ=255)                                                                                                    cGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGACGCCGCTATTGCCCGCGCCAAAG‑CAAGCAA‑GCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACA‑GGTCGATCCGCGCAAAGCCGCCGTCACCGCCGGTCTTGCCCGAGCCACAGCCCGCACACGGGAACAGCAACCGGCTGCCGCGGAGccctacagacaggtc                                                                                                                                                                                        >  1:150208/1‑276 (MQ=14)                                                                                                    cGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAG‑CACGCAA‑GCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACA‑GGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGtt                                                                                                                                                                                        >  2:260936/1‑289 (MQ=255)                                                                                                           cgtaaaactgccgTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCAGCCGTCGAAGCCGCTATTGCCCGTGCCAAAG‑CCCGCAA‑GCAGGAACAGCAACAGGCTAATGCGTGACCAGAAGAACA‑GGTTGATCCGCGCAAAGCCGCCGTCGAAGCGGCTAGTGCCGGTGCCCAAGCGAGCCAACTGGAAAAGAAACCAGAACATGCGAAGCCCAAATAACAgatcgagacgg                                                                                                                                                                                 >  1:519002/10‑279 (MQ=14)                                                                                                           cgcAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAG‑CACGCAA‑GCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACA‑GGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCCATTCCCCGAGCCTAAGCCCGCAAACCGGAACAGCAACCCGCTAATGCCGAGCCAGAAGAACAGGTTGATcac                                                                                                                                                                                  >  2:660558/1‑286 (MQ=255)                                                                                                                           aaGACGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACAAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAG‑CACGCAA‑GCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACA‑GGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGa                                                                                                                                                                 <  2:436405/289‑1 (MQ=255)                                                                                                                            aGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAAAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAG‑CACGCAA‑GCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACA‑GGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGa                                                                                                                                                                 <  2:783583/288‑1 (MQ=255)                                                                                                                              agtatagtgagggcagaaaaacgggaaaGATGGAACAGAAAGAGGCTAAGGGGGAACAAGAAGAACAGACGGATCCGCTCAAAGCCGCCATCGAAGACGCTGTTGCCCGAGCCAAAG‑CACGCAA‑GCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACA‑GGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAg                                                                                                                                                               <  1:696976/262‑1 (MQ=14)                                                                                                                                  tATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAG‑CACGCAA‑GCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACA‑GGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCt                                                                                                                                                          >  2:848169/1‑289 (MQ=255)                                                                                                                                      gCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAG‑CACGCAA‑GCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACA‑GGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTAtt                                                                                                                                                       >  2:649955/1‑288 (MQ=255)                                                                                                                                        cggggCCAAAGCGGCAAAGATAGAACAGAAACAGGCTAATGCGGAACCAGAAAGAAAGGTCGACCCCCGAAAAACTGCCGTCGAAGCAGCTATGGCCCGGGCCAAAG‑CGGGCAA‑GCGGGAAGAGCAACAGGCTAATGCGGAGCCAGAAAAACA‑GGGCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAGCGGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCa                                                                                                                                                    <  1:597375/285‑1 (MQ=11)                                                                                                                                        acgcgcaaaaGCACGCAAACCGGAACAGCAACAGCCAAATGCGGACCCAACACAAGTGGTCGGTCCGGGCAAATCCGCCGTGGAGGCAGCTATTGCCCGGGCAAAAG‑CAGGCAA‑GCTGGACCAGCAACAGGAAAATGCGGAGCCAGAACAACT‑GGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCa                                                                                                                                                    <  1:453487/282‑1 (MQ=14)                                                                                                                                           gtacgacgggctgaaGCGGGAGCGGAAACAGGCAAATTGGGACCAAGAAAACCGAGGCGACCCGCGTAAAACGGCCGCAGAGGCCGCTCTTGCCAGAGCCAAAG‑CACGCAA‑GCTGGAACAGCAACAGGATAATGCCGAGCCAGAACACCA‑TGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCAAAAGCGCGCAAACGGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCAcg                                                                                                                                                  <  1:76790/275‑1 (MQ=14)                                                                                                                                            gCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAG‑CACGCAA‑GCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACA‑GGTCGATCCGCGCAACGCCGCCGTTGAACCCGCCAATGCCCGCGCCAACGGCCGCAAACCGGACGAGCAACAGGCTAATGCGGAACCAGAAGCAGAGGTTGAGCCGCGCAACGCTGCGGTcactccgctgcatgcccgc                                                                                                                                                 >  1:98108/1‑270 (MQ=14)                                                                                                                                              cAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAG‑CACGCAA‑GCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACA‑GGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGc                                                                                                                                                     >  2:843952/1‑282 (MQ=255)                                                                                                                                               aatgtggcgaagagagaagaacAACAGGATAATAACGAACCAGAACGACAGGTCGATCCACGCAAAGCCGCCGTGGAAGCCGCTATGGCCCGGGCCAAAG‑CGAGCAA‑GATGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACA‑GGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCa                                                                                                                                             <  1:584913/268‑1 (MQ=14)                                                                                                                                                     cgcAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAG‑CACGCAA‑GCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACA‑GGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTCATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGc                                                                                                                                                     >  2:696976/1‑275 (MQ=255)                                                                                                                                                      gcgaGCTGGAACAGAAACAGGCGAATGCGGAACCAGAAACACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAG‑CACGCAA‑GCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACA‑GGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACg                                                                                                                                      <  2:202016/286‑1 (MQ=255)                                                                                                                                                            tGGCACAACAACAGGCTAATGCCGAACCAGAAAAACAGGTCGATCCGCGGAAAACGGCCGTCGAAGCCGCTATTGCCCGTGCCAAAG‑CACGCAA‑GCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACA‑GGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGc                                                                                                                                 <  2:590759/288‑1 (MQ=255)                                                                                                                                                                aCAGCAACAGGCTAATGCGGAACCAGAAGAACAGGACGATCCGCCGAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAG‑CACGCAA‑GCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACA‑GGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGaa                                                                                                                            <  2:29565/289‑1 (MQ=255)                                                                                                                                                                    cAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAG‑CACGCAA‑GCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACA‑GGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGc                                                                                                                        >  2:494413/1‑289 (MQ=255)                                                                                                                                                                    cAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAG‑CACGCAA‑GCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACA‑GGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCAAGCAAGCTGGAACAGc                                                                                                                        >  2:730161/1‑289 (MQ=255)                                                                                                                                                                     aaCAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAG‑CACGCAA‑GCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACA‑GGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCag                                                                                                                                                                                                   >  2:221744/1‑213 (MQ=255)                                                                                                                                                                     aaCAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAG‑CACGCAA‑GCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACA‑GGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCag                                                                                                                                                                                                   <  1:221744/213‑1 (MQ=255)                                                                                                                                                                     aaCAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAG‑CACGCAA‑GCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACA‑GGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGt                                                                                                                                                                                         >  2:114421/1‑223 (MQ=255)                                                                                                                                                                      acgtgCTAATGCGGAACCAGAAAAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAG‑CACGCAA‑GCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACA‑GGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCaa                                                                                                                      <  2:519002/285‑1 (MQ=255)                                                                                                                                                                      aCAGGAAAATGCGGAACCAGAAGACCCGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAG‑CACGCAA‑GCTGGAACAGCAACAGGCTAATGCCGAACCAGAACAACA‑GGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCaa                                                                                                                      <  2:224526/289‑1 (MQ=255)                                                                                                                                                                         ggCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAG‑CACGCAA‑GCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACA‑GGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGCAGCGGCTATTGCACGCGCCAAAGCGCGCACGCTGGAACAGCACCAg                                                                                                                   >  2:264099/1‑289 (MQ=255)                                                                                                                                                                              aTGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAG‑CACGCAA‑GCTGGAACAGCAACAGGCTAATGCCGAGCCAGAAAAACA‑GGTCGATCCGCGCAAAGCCGCCGTCGACGCCGCCATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAAAGCGGAGCCAGAAAAACAGGTCTAGCCGCCCAAAGCTGCCGTACAAGATGCTATTGCCCGCGCCCAGGCCAGAAAGATGGCCCAGCACCACGCtca                                                                                                              >  1:609812/1‑287 (MQ=14)                                                                                                                                                                                              ccgagtccgcaacgcgacaaccgacgGCGAAGCCGCTATTGCCCGTGCCAAAG‑CACACAA‑GCCGGAACAGCAACAGGCTAATGCCGAGCCAGAAGACCA‑GGGCGATCGGCGCAAAGCCGCCGCCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAGCCGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTCGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAgaagaa                                                                                              <  1:649955/269‑1 (MQ=17)                                                                                                                                                                                                   tCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAG‑CACGCAA‑GCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACA‑GGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCCGGAACAGACACAGGCTAATGCGGTACCAGAAGAACAGGt                                                                                         >  2:656528/1‑289 (MQ=255)                                                                                                                                                                                                    cGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAG‑CACGCAA‑GCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACA‑GGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGtt                                                                                        >  2:455846/1‑289 (MQ=255)                                                                                                                                                                                                    cGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAG‑CACGCAA‑GCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACA‑GGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCAGTCGAAGCGGCTATTGCCCGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGtg                                                                                        >  2:639519/1‑288 (MQ=255)                                                                                                                                                                                                            gcAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAG‑CACGCAA‑GCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACA‑GGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCcgc                                                                                 >  2:744042/1‑288 (MQ=255)                                                                                                                                                                                                                aGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAG‑CACGCAA‑GCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACA‑GGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCaaa                                                                            >  2:673217/1‑289 (MQ=255)                                                                                                                                                                                                                    cctgtagtactgacTATGGCCGGTGCAAAAG‑CA‑GCAACGCTGGACAAGTAACAGGCCACTGCCGAGCCAG‑AGAACAGGGAGGCTCCGCGCAGAGCCGCCGTCGATGCCGCTATTGCCCGAGCCAAAGCGCGCAAACCTGAACAGCAACCGGCTAATGCAGAGCCAGAAGAGCAGGTGGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTTGATCCGCGCAAAGcc                                                                         <  1:673217/275‑2 (MQ=14)                                                                                                                                                                                                                      cgTCGAAGCCGCTATTGCCCGTGCCAAAG‑CACGCAA‑GCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACA‑GGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGAGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTTGACCCGGCCATTGCCCGCGCCCAGGCACGCAAGCCGGCACAGCAACAGGCAAATGCGGCAGCAACAGCAAAGGTTGCACCgcgccgaccagcc                                                                      >  1:3594/1‑280 (MQ=17)                                                                                                                                                                                                                              tcggccttGCCCGTGCAAAAGACAC‑CAA‑GCTGTAACAGCAAAAGGCTAAGGACGAACACGACGAACA‑GGTCGATCCGGGCAAAGCGGCAGTCGAAGCCGGTATGGCCCGAGCCAAAGCGCGAAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGGTCGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGTGCAAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTTGCcg                                                               <  1:684685/282‑1 (MQ=14)                                                                                                                                                                                                                               cGCTATTGCCCGTGCCAAAG‑CACGCAA‑GCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACA‑GGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTTGCcg                                                               <  2:871143/287‑1 (MQ=255)                                                                                                                                                                                                                                 cTATTGCCCGTGCCAAAG‑CACGCAA‑GCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACA‑GGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGa                                                                                                                                                                 >  1:610104/1‑187 (MQ=255)                                                                                                                                                                                                                                 cTATTGCCCGTGCCAAAG‑CACGCAA‑GCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACA‑GGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGa                                                                                                                                                                 <  2:610104/187‑1 (MQ=255)                                                                                                                                                                                                                                    ttGCCCATGCCCAAG‑CACGCAA‑GCTGGAAAAGCAAGAGGCTAATGCCGAGCCAGAACAACA‑GGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTCGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTTGCCGCGGCTAt                                                        <  2:573390/289‑1 (MQ=255)                                                                                                                                                                                                                                      tcagagagtaataagagacagagacggAGCAGCAAACGGCTAATTCCGGGCCACAACAACA‑TGTCGTGCCGCGCAAAGCCGCCGTCGAAGCCTCTAGTGCCCGGTCCAACGCGCGAAAACTGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCACAAAGCTGCCGTCGAAGCCGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTTGCCGCGGCTAt                                                        <  1:481946/264‑1 (MQ=17)                                                                                                                                                                                                                                          gTGCCAAAG‑CACGCAA‑GCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACA‑GGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTTGCCGCGGCTATTGcc                                                    >  1:74394/1‑287 (MQ=255)                                                                                                                                                                                                                                          gTGCCAAAG‑CACGCAA‑GCTGGAACAGCAACAGGCTAATGCCAAGCCAGAACAACA‑GGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTTGCCGCGGCTATTGcc                                                    <  2:74394/287‑1 (MQ=255)                                                                                                                                                                                                                                           tGCCAAAG‑CACGCAA‑GCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACA‑GGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTTGCCGCGGCTATTGCCcg                                                  >  2:739649/1‑288 (MQ=255)                                                                                                                                                                                                                                            gCCAAAG‑CACGCAA‑GCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACA‑GGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTTGCCGCGGCTATTGCCcgc                                                 >  2:256151/1‑288 (MQ=255)                                                                                                                                                                                                                                             ccAAAG‑CACGCAA‑GCTGGAACAGCAACAGGCTAATG‑CGAGCCAGAACAACA‑GGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTTGCCGCGGCTATTGCCCGCGCt                                              <  2:802136/289‑1 (MQ=255)                                                                                                                                                                                                                                              cAAAG‑AACGAGA‑GCTGGAACAGCAACAGGCTAATGCCGAGCCAGAAAAACA‑GGTCGATCCGCGCAAAGCCGCCGTCGAAGACGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAAAAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTTGCCGCGGCTATTGCCCGCGCt                                              <  2:685176/289‑1 (MQ=255)                                                                                                                                                                                                                                                                      cAACAGGCTAATGCCGAGCCAGAACAACA‑GGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAgcgcg                                                                                                                                                                                                                                      >  2:91990/1‑83 (MQ=18)                                                                                                                                                                                                                                                                      cAACAGGCTAATGCCGAGCCAGAACAACA‑GGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAgcgcg                                                                                                                                                                                                                                      <  1:91990/83‑1 (MQ=18)                                                                                                                                                                                                                                                                      cAACAGGCTAATGCCGAGCCAGAACAACA‑GGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAAAAGGCCAATGCGGTACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTTGCCGCGGCTATTGCACgcgcc                                              >  2:481946/1‑266 (MQ=255)                                                                                                                                                                                                                                                                        aCAGGCTAATGCCGAGCCAGAACAACA‑GGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTTGCCGCGGCTATTGCCCGCGCTCAGGCCAAAAAAGCCGCCcagcag                      >  2:256933/1‑289 (MQ=255)                                                                                                                                                                                                                                                                            gCTAATGCGGAGCCAGAAGAACA‑GGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTTGCCGCGGCTATTGCCCGCGCt                                              <  2:348125/261‑1 (MQ=255)                                                                                                                                                                                                                                                                                    cGAGCCAGAACAACA‑GGTCGATCCGCGCAAGGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTTGCCGCGGCTATTGCCCGCGCTCAGGCCAAAAAAGCCGCCCAGCAGAAGGTTGTAAAc          <  2:550563/289‑1 (MQ=255)                                                                                                                                                                                                                                                                                      aGCCAGAAGAACA‑GGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACTGCAACAGGCTAATGCGGTACCCGAAAAACAGGGTGATCCGCGCAAAGCGGCAGTTGCACCGGCAATTGACCGGGCCCCGGCCaaaaacggtcacttaaacccatcttacggtg         >  1:348125/1‑262 (MQ=17)                                                                                                                                                                                                                                                                                      aGCCAGAACAACA‑GGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCAc                                                                                                                                                   >  1:28186/1‑150 (MQ=255)                                                                                                                                                                                                                                                                                      aGCCAGAACAACA‑GGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCAc                                                                                                                                                   <  2:28186/150‑1 (MQ=255)                                                                                                                                                                                                                                                                                      aGCCAGAACAACA‑GGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTTGCAGCGGCTATTTCCCGCGCTACGGCCAAAAAAGCCGCCCAGCCGACGGTTGTAAACGa        >  1:253861/1‑289 (MQ=255)                                                                                                                                                                                                                                                                                       tccAGAACTACG‑GGTGCATCCGCTGAAAGCCGCCGTCGAAGCCGCTATTGCCCGGGCCAAAGCGCGCTAGTCGGAACAGCAACGAGCGAATGCGGAGCAAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCAAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTGGATCCGCGCAAAGCGGCAGTTGCCGCGGCTATTGCCCGCGCTCAGGCCAAAAAAGCCGCCCAGCAGAAGGTTGTAAACGa        <  1:639519/287‑1 (MQ=18)                                                                                                                                                                                                                                                                                       gCCAGACCAACA‑GGTAGATCCGCGCAAAGCCGCCGTCGAAGCCGCTAGTGCCCGCGCCAAAGCGCGCAAACGGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGAGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTTGCCGCGGCTATTGCCCGCGCTCAGGCCAAAAAAGCCGCCCAGCAGAAGGTTGTAAACGa        <  2:649696/288‑1 (MQ=255)                                                                                                                                                                                                                                                                                       gCCAGAACACCA‑GGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTTGCCGCGGCTATTGCCCGCGCTCAGGCCAAAAAAGCCGCCCAGCAGAAGGTTGTAAACGa        <  2:669249/288‑1 (MQ=255)                                                                                                                                                                                                                                                                                       gCCAGAACAACA‑GGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTTGCCGCGGCTATTGCCCGCGCTCAGGCCAAAAAAGCCGCCCAGCAGAAGGTTGTAAACGa        <  2:494437/288‑1 (MQ=255)                                                                                                                                                                                                                                                                                         cAGAACAACC‑GGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCACGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGGGCCAGAAGACCAGGTTGAGCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCAAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTTGCCGCGGCTATTGCCCGCGCTCAGGCCAAAAAAGCCGCCCAGCAGAAGGTTGTAAACGAGGa     <  2:771923/289‑1 (MQ=255)                                                                                                                                                                                                                                                                                            aacaacA‑GGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCCGTTGCCGCGGCTATTGCCCGCGCTCCGGCCAAAAAAGCCGCCCAGCCGCAGGTTGTAAGCGAggccga  >  1:99815/1‑285 (MQ=255)                                                                                                                                                                                                                                                                                            aacaacA‑GGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTTGCCGCGGCTATTGCCCGCGCTCAGGCCAAAAAAGCCGCCCAGCAGAAGGTTGTAAACGAGGACTa  >  2:613665/1‑289 (MQ=255)                                                                                                                                                                                                                                                                                             acaaca‑GGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTTGCCGCGGCTATTGCCCGCGCTCAGGCCAAAAAAGCCGCCCAGCAGAAGGTTGTAAACGAGGACt   <  2:574552/287‑1 (MQ=255)                                                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                                                                GCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAAC‑CAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAG‑CACGCAA‑GCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACA‑GGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTTGCCGCGGCTATTGCCCGCGCTCAGGCCAAAAAAGCCGCCCAGCAGAAGGTTGTAAACGAGGACTA  >  NC_000913/1708572‑1709140

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑