Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,627,233	G→T	3.6%	G441W (GGG→TGG)	radA →	DNA repair protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,627,233	0	G	T	3.6%	343.7 / 2.5	97	G441W (GGG→TGG)	radA	DNA repair protein
Reads supporting (aligned to +/- strand):  ref base G (41/52);  new base T (2/2);  total (43/54)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.25e-01

TCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGTGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGCGACAGACCGCTGCCGCAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGAC‑TTATAATGAGATATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCC‑ATCAAGCAACAGGGCTGCACGCTACAGC‑AGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTG  >  NC_000913/4626953‑4627517                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                                tCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGTGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGCGACAGACCGCTGCCGCAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAg                                                                                                                                                                                                                                                                                         >  2:562915/1‑289 (MQ=255) tCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGTGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGCGACAGACCGCTGCCGCAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAg                                                                                                                                                                                                                                                                                         >  2:379459/1‑289 (MQ=255)          gttcgaaaTGGCCGATCAGGAGGTGTTGTGGAAGCTGGTTGGCGGGCGGAAGGTAACCGAAACCGTTGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGCGACAGACCGCTGCCGCAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATACGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTgg                                                                                                                                                                                                                                                                                 <  1:794891/283‑1 (MQ=255)           tttcAAATGTCCGTTCAGGATGTGTTTGTGAACTTGTTCGGCGGCGTGAAGGTAACCGAAACCAGGGCCGAATTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGCGACAGACCGCTGCCGCAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCg                                                                                                                                                                                                                                                                               <  2:261552/286‑1 (MQ=255)           cTGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGACGGT‑ACCGAAACCAGTGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGCGACAGACCGCTGCCGCAGGATCTGGCGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCCGGAACGAATCCCTGAAGCGGCCAACCACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGGGCCGGAGGGGATGGAGCATTTTGGc                                                                                                                                                                                                                                                                                >  1:552319/1‑287 (MQ=255)           cTGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGTGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGCGACAGACCGCTGCCGCAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCg                                                                                                                                                                                                                                                                               >  2:738644/1‑289 (MQ=255)           cTGAAAATGGCCGATCAGGTGGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGTGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGCGACAGACCGCTGCCGCAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCg                                                                                                                                                                                                                                                                               <  1:300877/289‑1 (MQ=255)                 aTGGCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGTGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGCGACAGACCGCTGCCGCAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTaaaa                                                                                                                                                                                                                                                                         >  2:112901/1‑289 (MQ=255)                   ggCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGTGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGCGACAGACCGCTGCCGCAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAATGGATGCAGATTTTTGGCGTTaaaaaa                                                                                                                                                                                                                                                                       >  2:239703/1‑289 (MQ=255)                   ggCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGTGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGCGACAGACCGCTGCCGCAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTcaaaaa                                                                                                                                                                                                                                                                       >  1:279287/1‑289 (MQ=255)                              aTGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGTGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGCGACAGACCGCTGCCGCAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGa                                                                                                                                                                                                                                                               <  2:857576/286‑1 (MQ=255)                              aTGGGTTTGGGAACGTGGTCGGCGGCGGTAAGGTAACCGAAAGCGGTGCCGACGTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGCGACAGACCGCTGCCGCAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTGAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGa                                                                                                                                                                                                                                                               <  1:752548/286‑1 (MQ=255)                               tgtgTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGTGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGCGACAGACCGCTGCCGCAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAATGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGAcgc                                                                                                                                                                                                                                                            >  2:415571/1‑288 (MQ=255)                                   tttGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGTGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGCGACAGACCGCTGCCGCAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGa                                                                                                                                                                                                                                                               >  2:431466/1‑281 (MQ=255)                                   tttGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGTGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGCGACAGACCGCTGCCGCAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGa                                                                                                                                                                                                                                                               <  1:431466/281‑1 (MQ=255)                                       tgAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGTGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGCGACAGACCGCTGCCGCAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGACGGGATGCAGATTTTTGGCGTTCAAAAACTTCCCGACGCCCttcgggt                                                                                                                                                                                                                                                   >  1:627361/1‑284 (MQ=255)                                        gAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGTGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGCGACAGACCGCTGCCGCAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCTGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCgtg                                                                                                                                                                                                                                                  <  2:717811/289‑1 (MQ=255)                                             tGGTCGGCGGCGTGAAGGTAACCGAAACCAGTGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGCGACAGACCGCTGCCGCAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTcg                                                                                                                                                                                                                                              >  1:303860/1‑288 (MQ=255)                                              ggTCGGCGGCGTGAAGGTAACCGAAACCAGTGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGCGACAGACCGCTGCCGCAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTACCCGACGCGGTTAGCGGGTTcgac                                                                                                                                                                                                                                            >  1:77846/1‑289 (MQ=255)                                               ggcggcggCGTGAAGGTAACCGAAACCAGTGCCGACTTGACGTTACTGCTGGCGATGGTTTCCAGCCTGCGCGACAGACCGCGGCCGCAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAATGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTcgac                                                                                                                                                                                                                                            <  1:239703/286‑1 (MQ=255)                                               gTCGGCGGCGTGCAGGTAACCGAAACCAGTGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGCGACAGACCGCTGCCGCAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCTGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTcgac                                                                                                                                                                                                                                            <  2:682646/288‑1 (MQ=255)                                               gTCGGCGGCGTGAAGGTAACCGAAACCAGTGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGCGACAGACCGCTGCCGCAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTcgacg                                                                                                                                                                                                                                           >  2:619310/1‑289 (MQ=255)                                               gTCGGCGGCGTGAAGGTAACCGAAACCAGTGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGCGACAGACCGCTGCCGCAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTcgac                                                                                                                                                                                                                                            <  2:303860/288‑1 (MQ=255)                                               gTCGGCGGCGTGAAGGTAACCGAAACCAGTGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGCGACAGACCGCTGCCGCAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTcgac                                                                                                                                                                                                                                            <  2:537066/288‑1 (MQ=255)                                               gTCGGCGGCGTGAAGGTAACCGAAACCAGTGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGCGACAGACCGCTGCCGCAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTcgac                                                                                                                                                                                                                                            <  2:689824/288‑1 (MQ=255)                                                       cgTGAAGGTAACCGAAACCAGTGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGCGACAGACCGCTGCCGCAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGAC‑Ttata                                                                                                                                                                                                                                   <  2:494030/288‑1 (MQ=255)                                                       cgTGAAGGTAACCGAAACCAGTGCCGACTTAGCGTTACGGCTGGCGATGGTTTCCAGCCTGCGCGACAGACCGCTGCCGCAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGAC‑Ttata                                                                                                                                                                                                                                   <  2:727421/288‑1 (MQ=255)                                                         tGAAGGTAACCGAAACCATGGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGCGACAGACCGTGGCAGCAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGAC‑TTATAAt                                                                                                                                                                                                                                 <  1:822223/288‑1 (MQ=255)                                                         tGAAGGTAACCGAAACCAGTGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGCGACAGACCGCTGCCGCAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGAC‑TTATAAt                                                                                                                                                                                                                                 <  2:279287/288‑1 (MQ=255)                                                         tGAAGGTAACCGAAACCAGTGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGCGACAGACCGCTGCCGCAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGAC‑TTATAAt                                                                                                                                                                                                                                 <  2:623512/288‑1 (MQ=255)                                                                aaCCGAAACCAGTGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGCGACAGACCGCTGCCGCAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGAC‑TTATAATGAGAtata                                                                                                                                                                                                                         <  1:379459/289‑1 (MQ=255)                                                                    gAAACCAGTGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGCGACAGACCGCGGCCGCAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAATGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGAC‑TTATAATGAGATATACgga                                                                                                                                                                                                                     <  1:415571/289‑1 (MQ=255)                                                                      aaCCAGTGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGCGACAGACCGCTGCCGCAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGAC‑TTATAATGAGATATACggagg                                                                                                                                                                                                                   >  2:647985/1‑289 (MQ=255)                                                                       aCCAGTGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGCGACAGACCGCTGCCGCAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGAC‑TTATAATGAGATATACggaggg                                                                                                                                                                                                                  >  1:780850/1‑289 (MQ=255)                                                                            tGCCGACTTGGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGCGACAGACCGCTGCCGCAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGAC‑TTATAATGAGATATACGGAGGGAGat                                                                                                                                                                                                              <  1:693667/288‑1 (MQ=255)                                                                            tGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGCGACAGACCGCTGCCGCAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGAC‑TTATAATGAGATATACGGAGGGAGat                                                                                                                                                                                                              <  2:356348/288‑1 (MQ=255)                                                                                   ttAGGGGTACGGCGGGCGATGGTTTCCAGCCTGGGCGACAGACCGCGGCCGCAGGAGCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGAGCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGAC‑TTATAATGAGATATACGGAGGGAGATATgtcgtc                                                                                                                                                                                                      <  1:146534/289‑1 (MQ=255)                                                                                   ttAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGCGACAGACCGCTGCCGCAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGAC‑TTATAATGAGCTTAACGGAGGGAGATATttcgtc                                                                                                                                                                                                      >  1:281196/1‑289 (MQ=255)                                                                                          tACTGCTGGCGATGGTTTCCAGCCTGCGCGACAGACCGCTGCCGCAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGAC‑TTATAATGAGATATACGGAGGGAGATATGTCGTCATTTGAt                                                                                                                                                                                               <  1:844507/289‑1 (MQ=255)                                                                                                 ggCGATGGTTTCCAGCCTGCGCGACAGACCGCTGCCGCAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGAC‑TTATAATGAGATATACGGAGGGAGATATGTCGTCATTTGATTACCTGa                                                                                                                                                                                        >  2:353692/1‑289 (MQ=255)                                                                                                          ttCCAGCCTGGGGGAAAGACCGCGGCCGGAGGATCTGGTGGTGTTGGGTGAAGTCTGGCTGGCAGGGGCGAGCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGAC‑TTATAATGAGATATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCC‑a                                                                                                                                                                              <  1:422532/289‑1 (MQ=255)                                                                                                                  tGCGCGACAGACCGCTGCCGCAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGAC‑TTATAATGAGATATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCC‑ATCAAGCaa                                                                                                                                                                      >  1:870704/1‑289 (MQ=255)                                                                                                                   gcgcGACAGACCGCTGCCGCAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGAC‑TTATAATgaga                                                                                                                                                                                                                             <  2:646980/234‑1 (MQ=255)                                                                                                                   gcgcGACAGACCGCTGCCGCAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGAC‑TTATAATgaga                                                                                                                                                                                                                             >  1:646980/1‑234 (MQ=255)                                                                                                                   gcgcGACAGACCGCTGCCGCAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGAC‑TTATAATGAGATATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCC‑ATCAAGCAAc                                                                                                                                                                     >  1:827550/1‑289 (MQ=255)                                                                                                                    cgcgACAGACCGCTGCCGCAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGGTGCAGCTTTTTGGCTGTAAAAAACTCACCGACGCGCCGAGCGTGTTCGACGAC‑TTATAATGAGATATACGGAGGGAGATATGTCGGCAATTGATTACCTTAAACCTGAC‑ATCCAGCACCa                                                                                                                                                                    >  1:592015/1‑289 (MQ=255)                                                                                                                    cgcgACAGACCGCTGCCGCAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGAC‑TTATAATGAGATATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCC‑ATCAAGCAACa                                                                                                                                                                    >  1:774696/1‑289 (MQ=255)                                                                                                                    cgcgACAGACCGCTGCCGCAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCGCTCAAGCGGCGAACCACGTTTTTCGCCGGGGGATTGTTCCGTCGGCTAACTTGCTGAAAAACGGGCCGGCGGGGCTGCAGATTTTTGCGGGTCAAAAACCCCCCGACCCGC‑TAGCGTGTTCCTCCACTTTCTATTGCGATCTACGCAGGGAGACTTGTCGTCATATGTTGACATG‑AACCGGCCAATCAAGCAAcg                                                                                                                                                                    >  1:674099/1‑288 (MQ=255)                                                                                                                        aCAGACGGCTGCCTCAGGGTCTGGTGGTGTTTGGGGAAGGCGGGATGGCAGGGGAGAGACGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAAAACGGTTTTCGCAATGCGATTGTTCCGGCGGATAACGAGCCGAACAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGAC‑TTATAATGAGATAGACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCC‑ATCAAGCAACAggg                                                                                                                                                                 <  1:396004/288‑1 (MQ=255)                                                                                                                             cggcggcagCAGGATCGGGTGGTGTTTGGTGAAGTCGGGGTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGAC‑TTATAATGAGATATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCC‑ATCAAGCAACAGGGCTGCa                                                                                                                                                            <  1:874959/280‑1 (MQ=255)                                                                                                                             ccgcgaccGCGGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGACCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGCCGAAACCCGGTGTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGAC‑TTATAATGAGATATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCC‑ATCAAGCAACAGGGCTGCa                                                                                                                                                            <  1:793904/282‑1 (MQ=255)                                                                                                                             cccctgcccttggATCAGGTGGTGTTTGGGGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGAC‑TTATAATGAGATATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCC‑ATCAAGCAACAGGGCTGCa                                                                                                                                                            <  2:592015/277‑1 (MQ=255)                                                                                                                             ccGCTGCCGCAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGAC‑TTATAATGAGATATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCC‑ATCAAGCAACAGGGCTGCa                                                                                                                                                            <  1:413619/288‑1 (MQ=255)                                                                                                                             ccGCTGCCGCAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGAC‑TTATAATGAGATATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCC‑ATCAAGCAACAGGGCTGCa                                                                                                                                                            <  1:353692/288‑1 (MQ=255)                                                                                                                             ccGCTGCCGCAGGAGCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGAC‑TTATAATGAGATATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCC‑ATCAAGCAACAGGGCTGCa                                                                                                                                                            <  2:540222/288‑1 (MQ=255)                                                                                                                                   ccGCAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGCTGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGAC‑TTATAATGAGATATACGGAGGGGGATATGTCGTCATTTGATTACCTGAAAACTGCC‑ATAAAGCAACAGGGCTGCACGCTAca                                                                                                                                                     >  1:644261/1‑289 (MQ=255)                                                                                                                                                   tgtTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGa                                                                                                                                                                                                                                                               >  1:418229/1‑169 (MQ=255)                                                                                                                                                   tgtTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGa                                                                                                                                                                                                                                                               <  2:418229/169‑1 (MQ=255)                                                                                                                                                   tgtTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGAC‑TTATAATGAGATATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCC‑ATCAAGCAACAGGGCTGCa                                                                                                                                                            <  1:461936/266‑1 (MQ=255)                                                                                                                                                   tgtTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGAC‑TTATAATGAGATATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCC‑ATCAAGCAACAGGGCTGCa                                                                                                                                                            >  2:461936/1‑266 (MQ=255)                                                                                                                                                    gtTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGAC‑TTATAATGAGATATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCC‑ATCAAGCAACAGGGCTGCACGCTACAGC‑AGGTAGCTGATGCCa                                                                                                                                   <  2:302214/289‑1 (MQ=255)                                                                                                                                                     tttGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGAC‑TTATAATGAGATATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCC‑ATCAAGCAACAGGGCTGCa                                                                                                                                                            <  2:259102/264‑1 (MQ=255)                                                                                                                                                     tttGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGAC‑TTATAATGAGATATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCC‑ATCAAGCAACAGGGCTGCa                                                                                                                                                            >  1:259102/1‑264 (MQ=255)                                                                                                                                                     tttGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGAC‑TTATAATGAGATATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCC‑ATCAAGCAACAGGGCTGCACGCTACAGC‑AGGTAGCTGATGCCa                                                                                                                                   >  2:643671/1‑288 (MQ=255)                                                                                                                                                     tttGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGAC‑TTATAATGAGATATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCC‑ATCAAGCAACAGGGCTGCACGCTACAGC‑AGGTAGCTGATGCCa                                                                                                                                   >  1:858232/1‑288 (MQ=255)                                                                                                                                                           gAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGAC‑TTATAATGAGATATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCC‑ATCAAGCAACAGGGCTGCACGCTACAGC‑AGGTAGCTGATGCCAGCGGTa                                                                                                                             <  1:643671/288‑1 (MQ=255)                                                                                                                                                                cGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGAC‑TTATAATGAGATATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCC‑ATCAAGCAACAGGGCTGCACGCTACAGC‑AGGTAGCTGATGCCAGCGGTATGAc                                                                                                                         <  2:281196/287‑1 (MQ=255)                                                                                                                                                                 gggCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTCGCGTGTTCGACGAC‑TTATAATGAGATATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCC‑ATCAAGCAACAGGGCTGCCCGCTACAGC‑AGGTAGCTGATGCCAGCGGTATGAccca                                                                                                                      >  2:570399/1‑287 (MQ=255)                                                                                                                                                                   gCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGAC‑TTATAATGAGATATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCC‑ATCAAGCAACAGGGCTGCACGCTACAGC‑Agg                                                                                                                                               <  2:456069/262‑1 (MQ=255)                                                                                                                                                                   gCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGAC‑TTATAATGAGATATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCC‑ATCAAGCAACAGGGCTGCACGCTACAGC‑Agg                                                                                                                                               >  1:456069/1‑262 (MQ=255)                                                                                                                                                                   gCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGAC‑TTATAATGAGATATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCC‑ATCAAGCAACAGGGCTGCACGCTACAGC‑AGGTAGCTGATGCCAGCGGTATGACCaaa                                                                                                                     <  2:858232/288‑1 (MQ=255)                                                                                                                                                                   gCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGAC‑TTATAATGAGATATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCC‑ATCAAGCAACAGGGCTGCACGCTACAGC‑AGGTAGCTGATGCCAGCGGTATGACCaaa                                                                                                                     <  2:123803/288‑1 (MQ=255)                                                                                                                                                                     tGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGAC‑TTATAATGAGATATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCC‑ATCAAGCAACAGGGCTGCACGCTACAGC‑AGGTAGCTGATGCCAGCGGTATGACCAAAgg                                                                                                                   <  2:774696/288‑1 (MQ=255)                                                                                                                                                                     tGGCAGGGGAGATCCGCCCGGGGCCCAGCGGTCAGGACCGAATCGCTGAAGCGGGGAAACACGGTTTTCGCCGGGGGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGAC‑TTATAATGAGATATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCC‑ATCAAGCAACAGGGCTGCACGCTACAGC‑AGGTAGCTGATGCCAGCGGTATGACCAAAgg                                                                                                                   <  1:738644/288‑1 (MQ=255)                                                                                                                                                                     gggCAGGGGAGAGCCGCCCGGCGCACGGGGGTAAGGAACGAATCTCTGAAGCGGCGGAACACGGATTGCGCCGGGCGATTGTTCCGGCGGCGAACGTGCCGAAAAAAGCGCGGGAAGGGATGGAGATTTTTGGCGTTAAAGAACTCTCCGACGCGCTTAGCGGGTTCGACGAC‑TTATAATGAGATATACGGAGGGAGATAGGTCGTCATTGGATTACCGGAGAACTGCC‑ATCAAGCAAAGGGGCTGCACGATACGGC‑AGGTAGCTGATGCCAGCGGTATGACCAAAgg                                                                                                                   <  1:800209/287‑1 (MQ=255)                                                                                                                                                                         aGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGACAAAAGCGCCGGCAGGGATGCAGATTTTTGGCGTTAAAACACTCTCCGACGCGCTTAGCGTGTTCGACGAC‑TTATAATGAGATATACGGAGGGAGATTTGTCGTCATTTGATTACCTGAATACTTCC‑ATCACGCAACATGGCCGCACGC‑CCATCTCGCTAGCTGCTGCCAGCGGGAGGACCGAAGGCTact                                                                                                              >  1:360152/1‑287 (MQ=255)                                                                                                                                                                                 tCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCT‑ACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGAC‑TTATAATGAGATATACGGCGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCC‑ATCAAGCAACAGGGCTGCACGCTACAGC‑AGGTAGCTGATGCCAGCGGTATGCCCAAAGGGTATTTAAGCCAGt                                                                                                     >  1:116871/1‑289 (MQ=255)                                                                                                                                                                                          tGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGAC‑TTATAATGAGATATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCC‑ATCAAGCAACAGGGCTGCACGCTACAGC‑AGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAAt                                                                                             >  2:845553/1‑289 (MQ=255)                                                                                                                                                                                          tGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGAC‑TTATAATGAGATATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGAC‑ATCAAGCAACAGGGCTGCACGCTACAGC‑AGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAAt                                                                                             >  1:65341/1‑289 (MQ=255)                                                                                                                                                                                                    taaGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGAC‑TTATAATGAGATATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCC‑ATCAAGCAACAGGGCTGCACGCTACAGC‑AGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATc                                                                                    <  2:552319/286‑1 (MQ=255)                                                                                                                                                                                                     cAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGAC‑TTATAATGAGATATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCC‑ATCAAGCAACAGGGCTGCACGCTACAGC‑AGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCaa                                                                                  <  2:870704/289‑1 (MQ=255)                                                                                                                                                                                                      aGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGAC‑TTATAATGAGATATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCC‑ATCAAGCAACAGGGCTGCACGCTACAGC‑AGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCaaa                                                                                 >  1:646894/1‑289 (MQ=255)                                                                                                                                                                                                              aTCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGAC‑TTATAATGAGATATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCC‑ATCAAGCAACAGGGCTGCACGCTACAGC‑AGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCa                                                                          >  2:814379/1‑288 (MQ=255)                                                                                                                                                                                                              aTCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGAC‑TTATAATGAGATATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCC‑ATCAAGCAACAGGGCTGCACGCTACAGC‑AGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAg                                                                         <  1:647985/289‑1 (MQ=255)                                                                                                                                                                                                              aTCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGAC‑TTATAATGAGATATACGGAGGGAGATATGTCGTCATTTGATTACATGAAAACTGCC‑ATCAAGCAACAGGGCTGCACGCTACAGC‑AGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGACTGCCAAAATCACAAGacccc                                                                          >  1:615549/1‑287 (MQ=255)                                                                                                                                                                                                                   tGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGAC‑TTATAATGAGATATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCC‑ATCAAGCAACAGGGCTGCACGCTACAGC‑AGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAgcgcgc                                                                    >  1:16531/1‑289 (MQ=255)                                                                                                                                                                                                                    gAAGCGGCGAGACACGGTTGTTCCCGGGAGATTGTGTCGCCGGCTAACGGGCCGAAAAAAGCGCCGAAAGGGATGTGGATTTTTGGCGTCAAAAACCTCTCCGACGCGGTCAGCGGGTGCGCCGAC‑TTAGAATGCGATATACGGAGGGAGATAGGTCGGCATTTGATTACCTGACAACTGCC‑ATCAAGCAACAGGGCTGCACGCTACAGC‑AGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATGTAGGCCAGTTACTGAATGCCAAAAGCAAAAGCCCCAGCGCGCa                                                                   <  1:31208/289‑1 (MQ=255)                                                                                                                                                                                                                        cggcgAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGAC‑TTATAATGAGATATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCC‑CTCAAGCAAAAGGGCTGCACGCTACAGC‑AGGTAGCTGATGCCCGCGGTATGACCAAAGGGTATTTAAGCCAGTTTCTGAATGCCAAAATCAAAAGCCCCCGCGCCCAAAAg                                                               >  1:771806/1‑289 (MQ=255)                                                                                                                                                                                                                          gcgAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGAC‑TTATAATGAGATATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCC‑ATCAAGCAACAGGGCTGCACGCTAc                                                                                                                                                      <  1:786016/201‑1 (MQ=255)                                                                                                                                                                                                                          gcgAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGAC‑TTATAATGAGATATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCC‑ATCAAGCAACAGGGCTGCACGCTAc                                                                                                                                                      >  2:786016/1‑201 (MQ=255)                                                                                                                                                                                                                          gcgAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGAC‑TTATAATGAGATATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCC‑ATCAAGCAACAGGGCTGCACGCTACAGC‑AGGTAGCTGATGCCAGCGGTATGACCAAAGGGTCTTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCaaaa                                                                >  2:452205/1‑286 (MQ=255)                                                                                                                                                                                                                          gcgAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGAC‑TTATAATGAGATATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCC‑ATCAAGCAACAGGGCTGCACGCTACAGC‑AGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCaaaa                                                                >  2:793700/1‑286 (MQ=255)                                                                                                                                                                                                                                                    tGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGAC‑TTATAATGAGATATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCC‑ATCAAGCAACAGGGCTGCACGCTACAGC‑AGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGc                                   <  2:16531/289‑1 (MQ=255)                                                                                                                                                                                                                                                        ccGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGAC‑TTATAATGAGATATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCC‑ATCAAGCAACAGGGCTGCACGCTACAGC‑AGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGa                               <  1:814379/289‑1 (MQ=255)                                                                                                                                                                                                                                                                              aaGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGAC‑TTATAATGAGATATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCC‑ATCAAGCAACAGGGCTGCACGCTACAGC‑AGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACg         <  2:77846/289‑1 (MQ=255)                                                                                                                                                                                                                                                                                  gcCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGAC‑TTATAATGAGATATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCC‑ATCAAGCAACAGGGCTGCACGCTACAGC‑AGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGAt       <  1:845553/287‑1 (MQ=255)                                                                                                                                                                                                                                                                                     ggAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGAC‑TTATAATGAGATATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCC‑ATCAAGCAACAGGGCTGCACGCTACAGC‑AGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGgtg  <  2:627361/289‑1 (MQ=255)                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                                TCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGTGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGCGACAGACCGCTGCCGCAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGAC‑TTATAATGAGATATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCC‑ATCAAGCAACAGGGCTGCACGCTACAGC‑AGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTG  >  NC_000913/4626953‑4627517

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑