BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,941,034	T→C	3.0%	E98G (GAG→GGG)	yieP ←	putative transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,941,034	0	T	C	3.0%	475.4 / 2.6	132	E98G (GAG→GGG)	yieP	putative transcriptional regulator
Reads supporting (aligned to +/- strand): ref base T (65/62); new base C (2/2); total (67/65)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.41e-01

Only 100 of 140 total aligned reads displayed.

TGGTAATTGACGTGAAGTAAGTGTGATAAACCGAATGGAATAGCGAGGCGAATGAGGTCAAAAACGGATTGGCGCTCATTTCATAGATATGCTCATGCCAGGCCATATCGACTTCGATCCAGCGTTCACGGCGAAAGTTCTCTTTTAATGCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACCCGAGTACCAATTCGCGGT‑CGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGACTCACTCCAAATTGCTCGCCCAGCTCAATCTCACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATACGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAG  >  NC_000913/3940750‑3941322

tGGTAATTGACGTGAAGTAAGTGTGATAAACCGAATGGAATAGCGAGGCGAATGAGGTCAAAAACGGATTGGCGCTCATTTCATAGATATGCTCATGCCAGGCCATATCGACTTCGATCCAGCGTTCACGGCGAAAGTTCTCTTTTAATGCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCtctt                                                                                                                                                                                                                                                                                                <  2:774988/288‑1 (MQ=255)

tGGTAATTGACGTGAAGTAAGTGTGATAAACCGAATGGAATAGCGAGGCGAATGAGGTCAAAAACGGATTGGCGCTCATTTCATAGATATGCTCATGCCAGGCCATATCGACTTCGATCCAGCGTTCACGGCGAAAGTTCTCTTTTAATGCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCtctt                                                                                                                                                                                                                                                                                                <  1:412363/288‑1 (MQ=255)

  gTAATTGACGTGAAGTAAGTGTGATAAACCGAATGGAATAGCGAGGCGAATGAGGTCAAAAACGGATTGGCGCTCATTTCATAGATATGCTCATGCCAGGCCATATCGACTTCGATCCAGCGTTCACGGCGAAAGTTCTCTTTTAATGCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGCCAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTctct                                                                                                                                                                                                                                                                                                 >  1:746111/1‑285 (MQ=255)

          cgggAAGTAAGTGTGATAAACCGAATGGAATAGCGAGGCGAATGAGGTCAAAAACGGATTGGCGCTCATTTCATAGATATGCTCATGCCAGGCCATATCGACTTCGATCCAGCGTTCACGGCGAAAGTTCTCTTTTAATGCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATccac                                                                                                                                                                                                                                                                                     <  2:688504/286‑1 (MQ=255)

               agtaagtGTGATAAACCGACTGGAAGAGCGAGGAGAATGAGGTCAAAAACGGATTTGCGCTCATTTCATAGATATGCTCATGCCAGGCCATATCGACTTCGATCCAGCGTTCACGGCGAAAGTTCTCTTTTAATGCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAgg                                                                                                                                                                                                                                                                                 <  2:505769/288‑1 (MQ=255)

                         aTAAACCGAATGGAATAGCGAGGCGAATGAGGTCAAAAACGGATTGGCGCTCATTTCATAGATATGCTCATGCCAGGCCATATCGACTTCGATCCAGCGTTCACGGCGAAAGTTCTCTTTTAATGCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCa                                                                                                                                                                                                                                                                                          >  2:870820/1‑269 (MQ=255)

                             aCCGAATGGAATAGCGAGGCGAATGAGGTCAAAAACGGATTGGCGCTCATTTCATAGATATGCTCATGCCAGGCCATATCGACTTCGATCCAGCGTTCACGGCGAAAGTTCTCTTTTAATGCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCCCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCACCCACCAGGTAAGCAATTCCTGa                                                                                                                                                                                                                                                                   >  2:19859/1‑288 (MQ=255)

                                 aaTGGAATAGCGAGGCGAATGAGGTCAAAAACGGATTGGCGCTCATTTCATAGATATGCTCATGCCAGGCCATATCGACTTCGATCCAGCGTTCACGGCGAAAGTTCTCTTTTAATGCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAg                                                                                                                                                                                                                                                              >  1:692361/1‑289 (MQ=255)

                                 aaTGGAATAGCGAGGCGAATGAGGTCAAAAACGGATTGGCGCTCATTTCATAGATATGCTCATGCCAGGCCATATCGACTTCGATCCAGCGTTCACGGCGAAAGTTCTCTTTTAATGCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAg                                                                                                                                                                                                                                                              >  1:176926/1‑289 (MQ=255)

                                        tAGCGAGGCGAATGAGGTCAAAAACGGATTGGCGCTCATTTCATAGATATGCTCATGCCAGGCCATATCGACTTCGATCCAGCGTTCACGGCGAAAGTTCTCTTTTAATGCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAAtt                                                                                                                                                                                                                                                        >  1:549516/1‑288 (MQ=255)

                                        tAGCGAGGCGAATGAGGTCAAAAACGGATTGGCGCTCATTTCATAGATATGCTCATGCCAGGCCATATCGACTTCGATCCAGCGTTCACGGCGAAAGTTCTCTTTTAATGCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAAtt                                                                                                                                                                                                                                                        >  1:864872/1‑288 (MQ=255)

                                           cGAGGCGAATGAGGTCAAAAACGGATTGGCGCTCATTTCATAGATATGCTCATGCCAGGCCATATCGACTTCGAGCCAGCGTTCACGGCGAAAGTTCTCTTTTAATGCCGCCATTTCGGCCATTAACGTATTGAGATTCGCTTTCTGCTCCGCGGTGCCCACCGTTGCTGCTAGGAGGCCGGCTCGCGGCTCCATACAGCACCGCATAACCAGAAAGTTATCGATGACATTATGAAAGTTCTCTTCTGTCCTACCCCAGGTAAGGACCTCCTGATCCAAAAAGTTCCAg                                                                                                                                                                                                                                                    >  2:705816/1‑289 (MQ=255)

                                              ggCGAATGAGGTCAAAAACGGATTGGCGCTCATTTCATAGATATGCTCATGCCAGGCCATATCGACTTCGATCCAGCGTTCACGGCGAAAGTTCTCTTTTAATGCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGtt                                                                                                                                                                                                                                                  <  1:755431/288‑1 (MQ=255)

                                               gCGAATGAGGTCAAAAACGGATTGGCGCTCATTTCATAGATATGCTCATGCCAGGCCATATCGACTTCGATCCAGCGTTCACGGCGAAAGTTCTCTTTTAATGCCGCCATTTCGGCCATTCACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACCGATACGCCTAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGACATTCCTGATCAAACAAATTCCCGTTCg                                                                                                                                                                                                                                                >  2:115193/1‑289 (MQ=255)

                                               gCGAATGAGGTCAAAAACGGATTGGCGCTCATTTCATAGATATGCTCATGCCAGGCCATATCGACTTCGATCCAGCGTTCACGGCGAAAGTTCTCTTTTAATGCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCg                                                                                                                                                                                                                                                >  1:67513/1‑289 (MQ=255)

                                                          cAAAAACGGATTGGCGCTCATTTCATAGATATGCTCATGCCAGGCCATATCGACTTCGATCCAGCGTTCACGGCGAAAGTTCTCTTTTAATGCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCAt                                                                                                                                                                                                                                      <  2:432230/288‑1 (MQ=255)

                                                          cAAAAACGGATTGGCGCTCATTTCATAGATATGCTCATGCCAGGCCATATCGACTTCGATCCAGCGTTCACGGCGAAAGTTCTCTTTTAATGCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCAt                                                                                                                                                                                                                                      <  1:8421/288‑1 (MQ=255)

                                                            aaaaCGGATTGGCGCTCATTTCATAGATATGCTCATGCCAGGCCATATCGACTTCGATCCAGCGTTCACGGCGAAAGTTCTCTTTTAATGCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGAc                                                                                                                                                                                                                                   <  1:109024/289‑1 (MQ=255)

                                                            aaaaCGGATTGGCGCTCATTTCATAGATATGCTCATGCCAGGCCATATCGACTTCGATCCAGCGTTCACGGCGAAAGTTCTCGTTTAATGCCGCCATTTCGTCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGAc                                                                                                                                                                                                                                   <  2:257804/289‑1 (MQ=255)

                                                                          cTCATTTCATAGATATGCTCATGCCAGGCCATATCGACTTCGATCCAGCGTTCACGGCGAAAGTTCTCTTTTAATGCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACCCGAGTACCAAtt                                                                                                                                                                                                                      >  1:170605/1‑288 (MQ=255)

                                                                               ttcatgattatgagcATGCCAGGGCATCTCGACTTCGGTCCAGCGTTTACGGCGAAAGTGCTCTTTTAATGCCGCCATTTCGGCCATTAACGTCTTGAGGTGCGCTTTGTGTTCCTCGGTGCCAACCGTTGCTGGCAGGAGGCAGGCTTGGGGCGCCAGACAGATACGGATAAGCAGAAAGTGATCGATGACCTGATGAAAGTTCGCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCGAGAAAATTCCAGTTCGATTGTGGCATGACCCGAGTACTATTtcgcggg                                                                                                                                                                                                                <  2:51255/275‑2 (MQ=255)

                                                                               ttCATAGATATGCTCATGCCAGGCCATATCGACTTCGATCCAGCGTTCACGGCGAAAGTTCTCTTTTAATGCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACCCGAGTACCAATtcgcggt                                                                                                                                                                                                                <  2:176926/289‑1 (MQ=255)

                                                                               ttCATAGATATGCTCATGCCAGGCCATATCGACTTCGATCCAGCGTTCACGGCGAAAGTTCTCTTTTAATGCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACCCGAGTACCAATtcgcggt                                                                                                                                                                                                                <  1:96399/289‑1 (MQ=255)

                                                                               ttCATAGATATGCTCATGCCAGGCCATATCGACTTCGATCCAGCGTTCACGGCGAAAGTTCTCTTTTAATGCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACCCGAGTACCAATtcgcggt                                                                                                                                                                                                                <  2:829205/289‑1 (MQ=255)

                                                                                       tatGCTCATGCCAGGCCATATCGACTTCGATCCAGCGTTCACGGCGAAAGTTCTCTTTTAATGCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACCCGAGTACCAATTcgcggt‑cgcggt                                                                                                                                                                                                         <  1:888468/287‑1 (MQ=255)

                                                                                       tatGCTCATGCCAGGCCATACCGACTTCGATCCAGCGTTCACGGCGAAAGTTCTCTTTTAATGCCGCAATTTCGGTCATTAAGGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCCTGCGGCTCCAGACAGACACGCATAACCAGAAAGTGATAGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTACTGCTCAAGAAAATTCCAGGCTGTTTGTGGGATGCCACGAGTCCCAATtcgcggcgtcggtga                                                                                                                                                                                                        >  2:591706/1‑280 (MQ=255)

                                                                                        atGCTCATGCCAGGCCATATCGACTTCGATCCAGCGTTCACGGCGAAAGTTCTCTTTTAATGCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACCCGAGTACCAATTCGCGGT‑CGCGGTaaa                                                                                                                                                                                                      >  2:614943/1‑289 (MQ=255)

                                                                                         tGCTCATGCCAGGCCATATCGACTTCGATCCAGCGTTCACGGCGAAAGTTCTCTTTTAATGCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGCAAGATACGCATAACCCGAAAGTGATCGATGACTTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGCAAATTCCAGTTCGATTTTGGCATGACCCGCATATCAATCTGCGGT‑CCCGGTaaaa                                                                                                                                                                                                     >  2:54001/1‑289 (MQ=255)

                                                                                         tGCTCATGCCAGGCCATATCGACTTCGATCCAGCGTTCACGGCGAAAGTTCTCTTTTAATGCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACCCGAGTACCAATtcgcggt                                                                                                                                                                                                                <  2:208574/279‑1 (MQ=255)

                                                                                          gCTCATGCCAGGCCATATCGACTTCGATCCAGCGTTCACGGCGAAAGTTCTCTTTTAATGCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACCCGAGTACCAATTCGCGGT‑CGCGGTaaaa                                                                                                                                                                                                     >  1:10521/1‑288 (MQ=255)

                                                                                                gCCAGGCCATATCGACTTCGATCCAGCGTTCACGGCGAAAGTTCTCTTTTAATGCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACCCGAGTACCAATTCGCGGT‑CGCGGTAAAACCATcc                                                                                                                                                                                               >  1:745095/1‑288 (MQ=255)

                                                                                                    ggCCATATCGACTTCGATCCAGCGTTCACGGCGAAAGTTCTCTTTTAATGCCGCCATTTCGGCCATTAACGCATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCCTGCGGCTCCAGACAGATACGCATAACCAGAAAGTCATCGATGACCTGATTAAAGTTCTCTTCTGTCATCTACCAGGTAAGCACTTCCTTATCAAGAAAATTACAGGTCGATTGTGGCATGAACCGACTACCAATTC‑CGGTGCCCGGTAAACCCATCCCtttt                                                                                                                                                                                          >  2:882996/1‑289 (MQ=255)

                                                                                                     gCCATATCGACTTCGATCCAGCGTTCACGGCGAAAGTTCTCTTTTAATGCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACCCGAGTACCAATTCGCGGT‑CGCGGTAAAACCATCCCtttt                                                                                                                                                                                          >  2:68077/1‑288 (MQ=255)

                                                                                                     gCCATATCGACTTCGATCCAGCGTTCACGGCGAAAGTTCTCTTTTAATGCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACCCGAGTACCAATTCGCGGT‑CGCGGTAAAACCATCCCtttt                                                                                                                                                                                          >  1:400368/1‑288 (MQ=255)

                                                                                                         tatCGACTTCGATCCAGCGTTCACGGCGAAAGTTCTCTTTTAATGCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGCTGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAACTTCCAGTTCGATTGTCGCATGACCCGAGTACCAATTCGCGGT‑CGCGGTAAAACCATACATTTTTCCGt                                                                                                                                                                                     >  2:456935/1‑289 (MQ=255)

                                                                                                          atCGACTTACATCCAGGGTTCACGGCGAAAGTTCTCTTTTAATGCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACCCGAGTACCAATTCGCGGT‑CGCGGTAAAACCATCCCTTTTGCCGtt                                                                                                                                                                                    <  2:378251/289‑1 (MQ=255)

                                                                                                                ttCGATCCAGCGTTCACGGCGAAAGTTCTCTTTTAATGCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACCCGAGTACCAATTCGCGGT‑CGCGGTAAAACCATCCCTTTTGCCGTTAACGtt                                                                                                                                                                              >  1:942612/1‑289 (MQ=255)

                                                                                                                   gATCCAGCGTTCACGGCGAAAGTTCTCTTTTAATGCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACCCGAGTACCAATTCGCGGT‑CGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTg                                                                                                                                                                           >  1:53923/1‑289 (MQ=255)

                                                                                                                        aGCGTTCACGGCGAAAGTTCTCTTTTAATGCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACCCGAGTACCAATTCGCGGT‑CGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCg                                                                                                                                                                       >  2:1000367/1‑288 (MQ=255)

                                                                                                                          cGTTCACGGCGAAAGTTCTCTTTTAATGCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACCCGAGTACCAATTCGCGGT‑CGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCtt                                                                                                                                                                    >  2:418443/1‑289 (MQ=255)

                                                                                                                          cGTTCACGGCGAAAGTTCTCTTTTAATGCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACCCGAGTACCAATTCGCGGT‑CGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCt                                                                                                                                                                     <  2:470868/288‑1 (MQ=255)

                                                                                                                           gTTCACGGCGAAAGTTCTCTTTTAATGCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACCCGAGTACCAATTCGCGGT‑CGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCt                                                                                                                                                                     <  1:812831/287‑1 (MQ=255)

                                                                                                                              accGGCGAAGGTTCTCTTTTAATGCGGCCATTTCGGCCATTAACGTATTTGTATGCGCTTTCTGTGCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTTATGAAAGTTCTCTTCTGTCATCCACCAAGTACGCAATTCCTGATCAAGAAAATTCCAGTTCGGTTGTGGCATGACCCGAGTACCAATTCGCAGT‑CGCGGTAAAACCCTCCCTTTTGCCGTTAACGTTTTGACCGCTTcgcg                                                                                                                                                                <  2:692361/287‑1 (MQ=255)

                                                                                                                               tcgccGAAAGTGCTCTTTTAATGCGGCCCTTTCGGCCATCAACGTCTTGGGCTGCGCTTTCTGTTCCGCCGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATCCGCATAACCAGAAAGTCATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAAGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACCCGAGTACCAATTCGCGGT‑CGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTcgcg                                                                                                                                                                <  2:744547/284‑1 (MQ=255)

                                                                                                                               aCGGCGAAAGTTCTCTTTTAATGCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGCAAATTCCAGTTCGATTGTGGCATGACCCGAGTACCAATTCGCGGT‑CGCGGTAAAACCATCCCTTTTGCCGTTAACGCTTTGAcaccttgtcgt                                                                                                                                                               >  2:817994/1‑279 (MQ=255)

                                                                                                                                 ggCGAAAGTTCTCTTTTAATGCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACCCGAGTACCAATTCGCGGT‑CGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTAc                                                                                                                                                             <  1:59209/289‑1 (MQ=255)

                                                                                                                                           ctctTTTAATGCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACCCGAGTACCAATTCGCGGT‑CGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACg                                                                                                                                                    >  1:816328/1‑288 (MQ=255)

                                                                                                                                           ctctTTTAATGCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACCCGAGTACCAATTCGCGGT‑CGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACg                                                                                                                                                    >  1:193335/1‑288 (MQ=255)

                                                                                                                                            tgttttAGTGCCGCCATTGCGGCCATTAACGGATTGAAATGCGCTTGCTGTTCCGCGGGGCCAACCGGTGCTGCCATTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACCCGAGTACCAATTCGCGGT‑CGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGac                                                                                                                                                  <  2:746111/287‑1 (MQ=255)

                                                                                                                                            gtgttgaaTGCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGGTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACCCGAGTACCAATTCGCGGT‑CGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGac                                                                                                                                                  <  2:213354/283‑1 (MQ=255)

                                                                                                                                                   aTGCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACCCGAGTACCAATTCGCGGT‑CGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGACTGACTcc                                                                                                                                           >  1:771763/1‑289 (MQ=255)

                                                                                                                                                        gccATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACCCGAGTACCAATTCGCGGT‑CGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCt                                                                                                                                                                     <  1:238259/258‑1 (MQ=255)

                                                                                                                                                                 gCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACCCGAGTACCAATTCGCGGT‑CGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGACTCACTCCAAATTGCTCGCCCa                                                                                                                             >  1:781107/1‑289 (MQ=255)

                                                                                                                                                                      tAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCGAGAAAATTCCAGTTCGATTGTGGCATGACCCGAGTACCAATTCGCGGT‑CGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGACTCACTCCAAATTGCTCGCCCAGCTCa                                                                                                                        <  1:418443/289‑1 (MQ=255)

                                                                                                                                                                            aTTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGTATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACCCGAGTACCAATTCGCGGT‑CGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGACTCACTCCAAATTGCTCGCCCAGCTCAAtctc                                                                                                                   >  2:887395/1‑288 (MQ=255)

                                                                                                                                                                            aTTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACCCGAGTACCAATTCGCGGT‑CGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGACTCACTCCAAATTGCTCGCCCAGCTCAAtctc                                                                                                                   >  1:25185/1‑288 (MQ=255)

                                                                                                                                                                            aTTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACCCGAGTACCAATTCGCGGT‑CGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGACTCACTCCAAATTGCTCGCCCAGCTCAATCTCa                                                                                                                  <  2:67513/289‑1 (MQ=255)

                                                                                                                                                                             tggaagagcgcGTTCTGTGCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACCCGAGTACCAAGTCGCGGT‑GGCGGTAAAACCATCACTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGACTCACTCCAAATTGCTCGCCCAGCTCAATCTCAc                                                                                                                 <  2:413433/282‑1 (MQ=255)

                                                                                                                                                                              tGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACCCGAGTACCAATTCGCGGT‑CGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGACTCACTCCAAATTGCTCGCCCAGCTCAATCTCAcc                                                                                                                <  1:68077/289‑1 (MQ=255)

                                                                                                                                                                                  aTGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCCCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACCCGAGTACCAATTCGCGGT‑CGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGACTCACTCCAAATTGCTCGCCCAGCTCAATCTCACCGGGc                                                                                                            >  1:172590/1‑289 (MQ=255)

                                                                                                                                                                                     cgcTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACCCGAGTACCAATTCGCGGT‑CGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGACTCACTCCAAATTGCTTGCCCAGCTCAATCTCACCGGTCaaa                                                                                                         >  1:293032/1‑289 (MQ=255)

                                                                                                                                                                                        tttCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACCCGAGTACCAATTCGCGGT‑CGCGGTAAAACCATCCCTTTTGCCGtt                                                                                                                                                                                    <  1:396716/211‑1 (MQ=255)

                                                                                                                                                                                           cTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCGGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACCCGAGTACCAATTCGCGGT‑CGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGACTCACTCCAAATTGCTCGCCCAGCTCAATCTCACCGGGCAAAATGGTg                                                                                                   <  1:365457/289‑1 (MQ=255)

                                                                                                                                                                                            tGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACCCGAGTGCCAATTCGCGGT‑CGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGACTCACTCCAAATTGCTCGCCCAGCTCAATCTCACCGGGCAAAATGGTGc                                                                                                  <  2:576832/289‑1 (MQ=255)

                                                                                                                                                                                            tGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACCCGAGTACCAATTCGCGGT‑CGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGACTCACTCCAAATTGCTCGCCCAGCTCAATCTCACCGGGCAAAATGGTGc                                                                                                  <  1:115193/289‑1 (MQ=255)

                                                                                                                                                                                            tGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACCCGAGTACCAATTCGCGGT‑CGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGACTCACTCCAAATTGCTCGCCCAGCTCAATCTCACCGGGCAAAATGGTGc                                                                                                  <  1:100741/289‑1 (MQ=255)

                                                                                                                                                                                              ttCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAg                                                                                                                                                                                                                                                                             >  1:742556/1‑117 (MQ=255)

                                                                                                                                                                                              ttCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAg                                                                                                                                                                                                                                                                             <  2:742556/117‑1 (MQ=255)

                                                                                                                                                                                               gccGCGGTGCCAACCGGTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACCCGAGTACCAATTCGCGGT‑CGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGACTCACTCCAAATTGCTCGCCCAGCTCAATCTCACCGGGCAAAATGGTGCCg                                                                                                <  1:496642/287‑1 (MQ=255)

                                                                                                                                                                                                      tGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACCCGAGTACCAATTCGCGGT‑CGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCt                                                                                                                                                                     <  1:813945/212‑1 (MQ=255)

                                                                                                                                                                                                      tGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACCCGAGTACCAATTCGCGGT‑CGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCt                                                                                                                                                                     >  2:813945/1‑212 (MQ=255)

                                                                                                                                                                                                      tGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACCCGAGTACCAATTCGCGGT‑CGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGACTCACTCCAAATTGCTCGCCCAGCTCAATCTCACCGGGCAAAATGGTGCCGGGTTCata                                                                                        >  1:117504/1‑289 (MQ=255)

                                                                                                                                                                                                         cAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACCCGAGTACCAATTCGCGGT‑CGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGACTCACTCCAAATTGCTCGCCCAGCTCAATCTCACCGGGCAAAATGGTGCCGGGTTCATAtt                                                                                      <  1:614943/288‑1 (MQ=255)

                                                                                                                                                                                                                tgctgcCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACCCGAGTACCAATTCGCGGT‑CGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGACTCACTCCAAATTGCTCGCCCAGCTCAATCTCACCGGGCAAACAGGTGCCGGGTTAATATTCACAtttt                                                                              >  2:646642/1‑289 (MQ=255)

                                                                                                                                                                                                                tgctgcCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACCCGAGTACCAATTCGCGGT‑CGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGACTCACTCCAAATTGCTCGCCCAGCTCAATCTCACCGGGCAAAATGGTGCCGGGTTCATATTCACCtttt                                                                              >  1:285668/1‑289 (MQ=255)

                                                                                                                                                                                                                tgctgcCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACCAGAGTACCAATTCGCGGT‑CGCGGTCAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCTCTGTACGACTCACTCCAAATTGCTCGCCCAGCTCAATCTCACCGGGCAAACTGGTCCCGGGTTCTTATTCCACtttt                                                                              >  2:268129/1‑289 (MQ=255)

                                                                                                                                                                                                                  ctgcCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCCCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACCCGAGTACCAATTCGCGGT‑CGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGACTCACTCCAAATTGCTCGCCCAGCTCAATCTCACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTac                                                                            >  2:566437/1‑288 (MQ=255)

                                                                                                                                                                                                                       aGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACCCGAGTACCAATTCGCGGT‑CGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTcgcg                                                                                                                                                                <  2:838898/200‑1 (MQ=255)

                                                                                                                                                                                                                             caggcTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACCCGAGTACCAATTCGCGGT‑CGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGACTCACTCCAAATTGCTCGCCCAGCTCAATCTCACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTgc                                                                  <  2:781107/288‑1 (MQ=255)

                                                                                                                                                                                                                               ggcTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACCCGAGTACCAATTCGCGGT‑CGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGACTCACTCCAAATTGCTCGCCCAg                                                                                                                            >  2:165745/1‑228 (MQ=255)

                                                                                                                                                                                                                                                  aTACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACCCGAGTACCAATTCGCGGT‑CGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGACTCACTCCAAATTGCTCGCCCAGCTCAATCTCACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACAt                                            >  1:223393/1‑289 (MQ=255)

                                                                                                                                                                                                                                                     cGCATAACCAGAAAGTGTTCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACCCGAGTACCAATTCGCGGT‑CGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGACTCACTCCAAATTGCTCGCCCAGCTCAATCTCACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATACg                                         <  1:435285/289‑1 (MQ=255)

                                                                                                                                                                                                                                                       cATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACCCGAGTACCAATTCGCGGT‑CGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGACTCACTCCAAATTGCTCGCCCAGCTCAATCTCACCGGGCAAAATGGTGCCGGGTTCATATTc                                                                                     >  2:53908/1‑243 (MQ=255)

                                                                                                                                                                                                                                                       cATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACCCGAGTACCAATTCGCGGT‑CGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGACTCACTCCAAATTGCTCGCCCAGCTCAATCTCACCGGGCAAAATGGTGCCGGGTTCATATTc                                                                                     <  1:53908/243‑1 (MQ=255)

                                                                                                                                                                                                                                                        aTAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACCCGAGTACCAATTCGCGGT‑CGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGACTCACTCCAAATTGCTCGCCCAGCTCAATCTCACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATACGaaa                                      >  1:322247/1‑289 (MQ=255)

                                                                                                                                                                                                                                                          aaCCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACCCGAGTACCAATTCGCGGT‑CGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGACTCACTCCAAATTGCTCGCCCAGCTCAATCTCACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATACGAAAgg                                    >  1:890455/1‑289 (MQ=255)

                                                                                                                                                                                                                                                                     gATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACCCGAGTACCAATTCGCGGT‑CGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGACTCACTCCAAATTGCTCGCCCAGCTCAATCTCACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATACGAAAGGTTTTTCtgtg                          <  1:887395/288‑1 (MQ=255)

                                                                                                                                                                                                                                                                     gATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAAGTCCAGTTCGATTGTGGCATGACCCGAGTACCAATTCGCGGT‑CGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGACTCACTCCAAATTGCTCGCCCAGCTCAATCTCACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATACGAAAGGTTTTTCtgtg                          <  1:456935/288‑1 (MQ=255)

                                                                                                                                                                                                                                                                      aTCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACCCGAGTACCAATTCGCGGT‑CGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGACTCACTCCCAATTTCTCGCCCAGCTCAATCTCACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGAACCGTTGCGCCAGCTTCTCAGCCAGAACATACGAAAGGTTTTTCTGTGCa                        >  2:516143/1‑289 (MQ=255)

                                                                                                                                                                                                                                                                      aTCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACCCGAGTACCAATTCGCGGT‑CGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGACTCACTCCAAATTGCTCGCCCAGCTCAATCTCACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCa                                                  >  2:369407/1‑263 (MQ=255)

                                                                                                                                                                                                                                                                      aTCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACCCGAGTACCAATTCGCGGT‑CGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGACTCACTCCAAATTGCTCGCCCAGCTCAATCTCACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGACCATACGAAAGGTTTTTCtgttaa                        >  2:34159/1‑286 (MQ=255)

                                                                                                                                                                                                                                                                                  atgaAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACCCGAGTACCAATTCGCGGT‑CGCGGTAACACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGACTCCCTCCAAATTGCTCGCCCAGCTCACTCTCCCCGGGCAAAATGGTCCCGGGTTCATATTCACCTTTTAAGGTCCGTTGCTCCAGCTTCCACGCCAGAACCTACGAAAGGTTTCTCTGTGCAGCt                     >  2:911664/1‑280 (MQ=255)

                                                                                                                                                                                                                                                                                  atgaAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACCCGAGTACCAATTCGCGGT‑CGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTAc                                                                                                                                                             >  2:93475/1‑144 (MQ=255)

                                                                                                                                                                                                                                                                                  atgaAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACCCGAGTACCAATTCGCGGT‑CGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTAc                                                                                                                                                             <  1:93475/144‑1 (MQ=255)

                                                                                                                                                                                                                                                                                  atgaAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACCCGAGTACCAATTCGCGGT‑CGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGACTCACTCCAAATTGCTCGCCCAGCTCAATCTCACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATACGAAAGGTTTTTCTGTGCAGCTAACtgttgt            >  1:955158/1‑289 (MQ=255)

                                                                                                                                                                                                                                                                                          tctcTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACCCGAGTACCAATTCGCGGT‑CGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGACTCACTCCAAATTGCTCGCCCAGCTCAATCTCACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATACGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCtt      <  1:34159/287‑1 (MQ=255)

                                                                                                                                                                                                                                                                                          tctcTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACCCGAGTACCAATTCGCGGT‑CGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGACTCACTCCAAATTGCTCGCCCAGCTCAATCTCACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATACGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCtt      <  1:19859/287‑1 (MQ=255)

                                                                                                                                                                                                                                                                                          tctcTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACCCGAGTACCAATTCGCGGT‑CGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGACTCACTCCAAATTGCTCGCCCAGCTCAATCTCACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATACGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTaa    <  1:652158/289‑1 (MQ=255)

                                                                                                                                                                                                                                                                                          tctcTTCTCACATCCACCAGGTAACAAATTCCTGATCAAGAAAATTCCAGTTCGATTTGGGCATGACCCGAGTCCCAATTCGCGGT‑CGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGACTCACTCCAAATTGCTCGCCCAGCTCAATCTCACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATACGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTaa    <  2:188234/289‑1 (MQ=255)

                                                                                                                                                                                                                                                                                            tcttctGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACCCGAGTACCAATTCGCGGT‑CGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGACTCACTCCAAATTGCTCTCCCAGCTCAATCTCACCGGGCAAAATGGTGCCGGGTTAATATTCACCTTTTAAGATCCGTTGCGACAGCTTCTTAGCCAGAACATACGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAg  >  2:839936/1‑289 (MQ=255)

TGGTAATTGACGTGAAGTAAGTGTGATAAACCGAATGGAATAGCGAGGCGAATGAGGTCAAAAACGGATTGGCGCTCATTTCATAGATATGCTCATGCCAGGCCATATCGACTTCGATCCAGCGTTCACGGCGAAAGTTCTCTTTTAATGCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACCCGAGTACCAATTCGCGGT‑CGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGACTCACTCCAAATTGCTCGCCCAGCTCAATCTCACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATACGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAG  >  NC_000913/3940750‑3941322

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 10 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`