Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,665,702	0	C	A	27.7%	196.5 / 31.0	83	A37S (GCG→TCG)	gadX	acid resistance regulon transcriptional activator; autoactivator
Reads supporting (aligned to +/- strand):  ref base C (55/5);  new base A (0/23);  total (55/28)
Fisher's exact test for biased strand distribution p-value = 5.36e-16
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01
Rejected as consensus: Frequency below/above cutoff threshold.

AGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATG‑TGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGG‑AGAAAT‑AATGAAAGCGTGTCCCTTG‑AC‑ACGAATACAAAATTTTC‑AA‑CACAC‑TTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAA‑CC‑AGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAGTCAAATTGATTGATAAATAAATTTAAACGAAGCGGGAGAA  >  NC_000913/3665476‑3665912                                                                                                                                                                                                                                            |                                                                                                                                                                                                                     aGCATGTCTGCATAGCGAAGTTTTTTCTTCTGCGTTACTTGCGCATCTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATG‑TGCATGAAGATTTAATGCCGCCTCCTTGAGCATCGGG‑AGAAAT‑AATGAAAGCGTGTCCCTTG‑AC‑ACGAATACAAAATTTTC‑AA‑CACAC‑TTATCTACTCGAATTTGGC‑TTGCATCCGCa                                                                                                                                                                                                                     <  2:1930527/228‑1 (MQ=255)   cATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATG‑TGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGG‑AGAAAT‑AATGAAAGCGTGTCCCTTG‑AC‑ACGAATACAAAATTTTC‑AA‑CACAC‑TTATCTACTCGAATTTGGC‑TTGCATCCGCaaa                                                                                                                                                                                                                   >  1:942919/1‑228 (MQ=255)           cATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATG‑TGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGG‑AGAAAT‑AATGAAAGCGTGACCCTTG‑AC‑ACGAATACAAAATTTTC‑AA‑CACAC‑TTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAA‑CA‑AGGTc                                                                                                                                                                                                        >  1:1440665/1‑229 (MQ=255)              aagagagtttgttATTCGGCATTGGTTGCAAAACTTCAGAAAAAAAAGTAATACCTCTACAACAGTGAGGAACGATAAAAGAAGAACTTTTTTAATG‑TGCAGGAAGATTTACTCCCCCCTCCTGGAGCATCGGG‑AAAAAT‑AATGAAAGCGTGTCCCTTG‑AC‑ACAAAAAAAAAATTTCC‑AA‑CCCAT‑TTATCAACCAAATTTTGGC‑TTGCA‑CCCCAAAAA‑CCAAGGTCAcc                                                                                                                                                                                                     <  2:2576/218‑1 (MQ=255)                cGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATG‑TGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGG‑AGAAAT‑AATGAAAGCGTGTCCCTTG‑AC‑ACGAATACAAAATTTTC‑AA‑CACAC‑TTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAA‑CC‑AGGTCACCGc                                                                                                                                                                                                   >  1:1768404/1‑229 (MQ=255)                cGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATG‑TGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGG‑AGAAAT‑AATGAAAGCGTGTCCCTTG‑AC‑ACGAATACAAAATTTTC‑AA‑CACAC‑TTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAA‑CC‑AGGTAACCGc                                                                                                                                                                                                   >  1:421862/1‑229 (MQ=255)                               tGCGATTGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATG‑TGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGG‑AGAAAT‑AATGAAAGCGTGTCCCTTG‑AC‑ACGAATACAAAATTTTC‑AA‑CACAC‑TTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAA‑AC‑AGGTCACCGCCATTAAAATAGCGat                                                                                                                                                                                    >  1:348745/1‑229 (MQ=255)                                 cGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATG‑TGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGG‑AGAAAT‑AATGAAAGCGTGTCCCTTG‑AC‑ACGAATACAAAATTTTC‑AA‑CACAC‑TTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAA‑CC‑AGGTCACCGCCCTTAAAATAGCGata                                                                                                                                                                                   >  1:1677586/1‑228 (MQ=255)                                 cGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATG‑TGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGG‑AGAAAT‑AATGAAAGCGTGTCCCTTG‑AC‑ACGAATACAAAATTTTC‑AA‑CACAC‑TTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAA‑CC‑AGGTCACCGCCATTAAAATAGCGata                                                                                                                                                                                   >  1:492091/1‑228 (MQ=255)                                 cGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATG‑TGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGG‑AGAAAT‑AATGAAAGCGTGTCCCTTG‑AC‑ACGAATACAAAATTTTC‑AA‑CACAC‑TTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAA‑CC‑AGGTCACCGCCATTAAAATAGCGata                                                                                                                                                                                   >  1:1716427/1‑228 (MQ=255)                                   atatTTGCGTAACTCGCTGAAAACCAGAAATGTCTCTTCTAAGGTTAAGAACGAATATAGAAAATATTTTTTAATG‑TGTAGAAAGATTTATGTCCACCCCCTTGATCATCGGA‑AGAATA‑AAGCAAAGGGT‑TCCCTTGCAC‑AAAAACCAAAAATTCTC‑AA‑CACAT‑TTATTTATTTAATTTTGTC‑TTCCACCCAAAAAAC‑CC‑GGGTCCCCGCCATTAAAATAGCGATATTc                                                                                                                                                                                <  2:1185709/229‑1 (MQ=255)                                            cAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATG‑TGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGG‑AGAAAT‑AATGAAAGCGTGTCCCTTG‑AC‑ACGAATACAAAATTTTC‑AA‑CACAC‑TTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAA‑CC‑AGGACACCGCCATTAAAATAGCGATATTCACCATTAAc                                                                                                                                                                       >  1:834329/1‑229 (MQ=255)                                              aCTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATG‑TGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGG‑AGAAAT‑AATGAAAGCGTGTCCCTTG‑AC‑ACGAATACAAAATTTTC‑AA‑CACAC‑TTATCTAATCGAATTTGGC‑TTGCATCCGCAAAAA‑CC‑AGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCa                                                                                                                                                                     >  1:843340/1‑229 (MQ=255)                                                  tataaaaaaCAGAAATATCTTTAATAAAGTGAAGAAAAAAAAAGAAAAAATCTTTTTTATG‑GTAATGAATTTTTATGCCCCCCTCCTTGGGCACCGGA‑AAAAAA‑AATAAAAGCTTTCCCTTTA‑AC‑AAGAATAAAAAATTCCA‑AA‑CACAC‑TTATCTACTCAATTTTTGC‑TTCCACCAGAAAAAC‑CC‑AGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGt                                                                                                                                                                 <  2:1438196/225‑1 (MQ=255)                                                  cagaaaaaaCAGAAATTCCCATACTACATTGATCAACTAGTAAGAAAAAATCTTTTTTATG‑TACATAAATTTTTACTCCCTCCCCCTAGAGCATGGGA‑AAAAAA‑AAAGAAGCCGTCCCCCTTG‑CC‑ACGAAAAAAAAATTTCC‑CA‑CCCCC‑TTATCTCCTC‑AATTTTGC‑TTTCCCCCCAAAAAACCC‑AGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATgg                                                                                                                                                                  <  2:421109/224‑1 (MQ=255)                                                  agtaaaaaaCAGAAATATCTCACCGAAAGTGATGAACGATTAAGAAAGAAA‑TTTTTTATGTTGAAGGAAGATTGAATGCCTCCTCATGGAGCATCGGA‑AAAAAT‑AATGAAAGCGTTTCCCTTG‑AC‑ACGAATACAAAATTTCC‑AC‑CACAC‑TTTTCTACTTGATTTTGTT‑TTCCACCCAAAAAAA‑CC‑AGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGt                                                                                                                                                                 <  2:216872/225‑1 (MQ=255)                                                                            caaggatgaATGAGTTATGAAAAAACTTTTTTTTG‑GGGAATAAATTTTTATTCCCTCTCCTTGGGAAGCGGGAAAAAAT‑AAGAAAGGCGGCTCCTTGG‑CC‑AC‑AAAAAAAAATTTTA‑AACCCCCC‑TTATCTATTAAAATTTGTT‑TTCCACCCGAAAAAC‑CC‑AGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATa                                                                                                                                         <  2:1544970/224‑1 (MQ=255)                                                                             aGTGATGAACGAGTAATGAAGAAACTTTTTTATG‑TGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGG‑AGAAAT‑AATGAAAGCGTGTCCCTTG‑AC‑ACGAATACAAAATTTTC‑AA‑CACAC‑TTATCTACTCGAATTTTGC‑TTGCATCCGCAAAAA‑CC‑AGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGc                                                                                                                                      >  2:800321/1‑229 (MQ=255)                                                                               tgatgaACGAGTAATGAAGAAACTTTTTTATG‑TGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGG‑AGAAAT‑AATGAAAGCGTGTCCCTTG‑AC‑ACGAATACAAAATTTTC‑AA‑CACAC‑TTATCTACTCGAATTTGGC‑TTGCATCCGCa                                                                                                                                                                                                                     >  1:1832442/1‑150 (MQ=255)                                                                               tgatgaACGAGTAATGAAGAAACTTTTTTATG‑TGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGG‑AGAAAT‑AATGAAAGCGTGTCCCTTG‑AC‑ACGAATACAAAATTTTC‑AA‑CACAC‑TTATCTACTCGAATTTGGC‑TTGCATCCGCa                                                                                                                                                                                                                     <  2:1829327/150‑1 (MQ=255)                                                                               tgatgaACGAGTAATGAAGAAACTTTTTTATG‑TGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGG‑AGAAAT‑AATGAAAGCGTGTCCCTTG‑AC‑ACGAATACAAAATTTTC‑AA‑CACAC‑TTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAA‑CC‑AGGTCAcc                                                                                                                                                                                                     >  1:1276682/1‑164 (MQ=255)                                                                               tgatgaACGAGTAATGAAGAAACTTTTTTATG‑TGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGG‑AGAAAT‑AATGAAAGCGTGTCCCTTG‑AC‑ACGAATACAAAATTTTC‑AA‑CACAC‑TTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAA‑CC‑AGGTCACCGCCATTAAAATAGCGATAtt                                                                                                                                                                                 >  1:519160/1‑184 (MQ=255)                                                                               tgatgaACGAGTAATGAAGAAACTTTTTTATG‑TGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGG‑AGAAAT‑AATGAAAGCGTGTCCCTTG‑AC‑ACGAATACAAAATTTTC‑AA‑CACAC‑TTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAA‑CC‑AGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCa                                                                                                                                     >  2:872463/1‑228 (MQ=255)                                                                               tgatgaACGAGTAATGAAGAAACTTTTTTATG‑TGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGG‑AGAAAT‑AATGAAAGCGTGTCCCTTG‑AC‑ACGAATACAAAATTTTC‑AA‑CACAC‑TTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAA‑CC‑AGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCa                                                                                                                                     >  1:527357/1‑228 (MQ=255)                                                                               tgatgaACGAGTAATGAAGAAACTTTTTTATG‑TGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGG‑AGAAAT‑AATGAAAGCGTGTCCCTTG‑AC‑ACGAATACAAAATTTTC‑AA‑CACAC‑TTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAA‑CC‑AGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCa                                                                                                                                     >  1:54682/1‑228 (MQ=255)                                                                               tgatgaACGAGTAATGAAGAAACTTTTTTATG‑TGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGG‑AGAAAT‑AATGAAAGCGTGTCCCTTG‑AC‑ACGAATACAAAATTTTC‑AA‑CACAC‑TTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAA‑CC‑AGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCa                                                                                                                                     >  1:115530/1‑228 (MQ=255)                                                                               tgatgaACGAGTAATGAAGAAACTTTTTTATG‑TGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGG‑AGAAAT‑AATGAAAGCGTGTCCCTTG‑AC‑ACGAATACAAAATTTTC‑AA‑CACAC‑TTATCTAATCGAATTTGGC‑TTGCATCCGCAAAAA‑CC‑AGGTCACCGCCATTAAAATAGCGATATTCACCa                                                                                                                                                                            >  1:1319447/1‑189 (MQ=255)                                                                               tgatgaACGAGTAAGGAAAAAACTTTTTAATG‑TGAATGAAGATTTAATGCCCCCTCCTTGAGCATGGGG‑AAAAAA‑AATGAAGGCGTGCCCCTTG‑AC‑AAAAAAAAAAAATTTCC‑AA‑CACCC‑TTTCCTCCTCAATTTTGGC‑TTCCACCCAAAAAAC‑CC‑AGGTCACCGCCATTAAAATAGCGATAtt                                                                                                                                                                                 <  2:518237/184‑1 (MQ=255)                                                                                 atgaACGAGTAATGAAGAAACTTTTTTATG‑TGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGG‑AGAAAT‑AATGAAAGCGTGTCCCTTG‑AC‑ACGAATACACAATTTTC‑AA‑CACGC‑TTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAA‑CC‑AGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAAt                                                                                                                                   >  1:1767646/1‑228 (MQ=255)                                                                                 atgaACGAGTAATGAAGAAACTTTTTTATG‑TGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGG‑AGAAAT‑AATGAAAGCGTGTCCCTTG‑AC‑ACGAATACAAAATTTTC‑AA‑CACAC‑TTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAA‑CC‑AGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATa                                                                                                                                         >  1:1547637/1‑222 (MQ=255)                                                                                 atgaACGAGTAATGAAGAAACTTTTTTATG‑TGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGG‑AGAAAT‑AATGAAAGCGTGTCCCTTG‑AC‑ACGAATACAAAATTTTC‑AA‑CACAC‑TTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAA‑CC‑AGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAAt                                                                                                                                   >  1:784033/1‑228 (MQ=255)                                                                                 atgaACGAGTAATGAAGAAACTTTTTTATG‑TGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGG‑AGAAAT‑AATGAAAGCGTGTCCCTTG‑AC‑ACGAATACAAAATTTTC‑AA‑CACAC‑TTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAA‑CC‑AGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAAt                                                                                                                                   >  1:689692/1‑228 (MQ=255)                                                                                 atgaACGAGTAATGAAGAAACTTTTTTATG‑TGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGG‑AGAAAT‑AATGAAAGCGTGTCCCTTG‑AC‑ACGAATACAAAATTTTC‑AA‑CACAC‑TTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAA‑CC‑AGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAAt                                                                                                                                   >  1:1132430/1‑228 (MQ=255)                                                                                    aaCGAGTAATGAAGAAACTTTTTTATG‑TGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGG‑AGAAAT‑AATGAAAGCGTGTCCCTTG‑AC‑ACGAATACAAAATTTTC‑AA‑CACAC‑TTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAA‑CC‑AGGTCACCGCCATTAAAATAGCGat                                                                                                                                                                                    >  1:1479393/1‑176 (MQ=255)                                                                                    aaCGAATAATGAAGAACTTTTTTTAGG‑GGAATGAAATTTTAATCCCTCCTCCTGAGGCAGCGGA‑AGAAAA‑AATAAAAGCGTGCCCCTTA‑GC‑AAAAAAAAAAAATTTCC‑AA‑CACTC‑TTATCTACTCGAATTTGGT‑TTCCACCCGAAAAAA‑CC‑AGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGa                                                                                                                               <  2:1895777/229‑1 (MQ=255)                                                                                      cGAGTAATGAAGAAACTTTTTTATG‑TGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGG‑AGAAAT‑AATGAAAGCGTGTCCCTTG‑AC‑ACGAATACAAAATTTTC‑AA‑CACAC‑TTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAA‑CC‑AGGTCACCGCCATTAAAATAGCGATAtt                                                                                                                                                                                 >  1:682557/1‑177 (MQ=255)                                                                                       gagaAATGAAAAATTTTTTTAAGG‑TACAAAAAATTTTAATCCCTCCTCCTTGGGCATGGGA‑AAAAAA‑AAAGAAAGCGTCTCCCTTA‑AC‑AGAAAAACAAAATTACA‑AA‑CACTT‑TTATCTATTGGATTTTGGT‑TTGCTTCCAAAAAAC‑CA‑AGGTATCCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACaa                                                                                                                            <  2:1130459/229‑1 (MQ=255)                                                                                       gaatAAAGAAAATACTTTATGAGG‑TGCATGAATATTTAACCCCTCCTACATGATCATCGGA‑AAAAAT‑AAAGAAGGCTGGTCCCTTG‑AC‑AAGAAAAAAAAATTTTC‑AA‑CACTC‑TTATCACCAAAATTTTGGT‑TTCCACCCAAAAAAA‑CC‑GGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACaa                                                                                                                            <  2:1765400/226‑1 (MQ=255)                                                                                         gTAATGAAGAAACTTTTTTATG‑TGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGG‑AGAAAT‑AATGAAAGCGTGTCCCTTG‑AC‑ACGAATACAAAATTTTC‑AA‑CACAC‑TTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAA‑CC‑AGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAAtt                                                                                                                          >  1:1823456/1‑229 (MQ=255)                                                                                            aaaaaaaattttttttGTG‑TGAAAGAATATTTAATCCCCCCTCCTGGAGAGGGGGG‑AAAAAT‑AATGAAGGCGT‑TCCCTTT‑AA‑CAAAAAAAAAAATTTTA‑AA‑CCCCCTTTATCTCCTAAATTTGGGT‑TTCCACCCAAAAAAC‑CC‑GGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTcc                                                                                                                        <  2:1746354/218‑1 (MQ=255)                                                                                                 gaaACTTTTTTATG‑TGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGG‑AGAAAT‑AATGAAAGCGTGTCCCTTG‑AC‑ACGAATACAAAATTTTC‑AA‑CACAC‑TTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAA‑CC‑AGGTCACCGCCATTAAAATAGCGATAtt                                                                                                                                                                                 >  1:29953/1‑166 (MQ=255)                                                                                                  acttttttttATG‑GATAGGAAGTTTAATTCCCCCCTCCTGAAGAGTGGGG‑AAAAAA‑AAAGAACGCGTGTCCCTTA‑AC‑AAGAA‑AAAAAATTTTCAAA‑CCCAC‑TTATTTATACGTTTTTGGT‑TTCCATCCAAAAAAA‑CG‑AGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACaa                                                                                                                            <  2:688483/214‑1 (MQ=255)                                                                                                   ctcTTTTTTATG‑TAAATAAATTTTGTATCCCCCCTCCGTGACCAGGGGA‑AAAAAA‑AAGAAAAGCGTCTCCTTTG‑AC‑AGAAATAAAAAATTTCA‑AA‑CCCCT‑TTATCAATGAGATTTTTGT‑TTGCCCCCAAAAAAA‑CC‑GGGCCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAg                                                                                                                 <  2:1764642/226‑1 (MQ=255)                                                                                                               gacaaTGAATATTTATCGCCCCCTCCTGAAGCATGGGG‑AAAAAT‑AATAAAGGCGTTTCCCTAA‑AA‑ACAAAAAAAAAATTTAA‑AA‑CACAC‑TTATCGCCT‑GAATTTTGG‑TTACCACCCAAAAAA‑CCAAGGAAACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGt                                                                                                     <  2:1722271/225‑1 (MQ=255)                                                                                                                    tGAATATTAAATCCCTCCTCCTTGA‑CAATGGG‑AAAAATAAATGAAAGCTTTCCCCTTG‑AC‑ACAAAAAAAAAATTTCA‑AA‑CCCAT‑TTTTTTAATCGATTTGGGC‑TTCCACCAAAAAAAA‑CC‑GTCTCTCCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACt                                                                                                <  2:491223/229‑1 (MQ=255)                                                                                                                                 cctcctcctTGAGAATCGGA‑AAAAAT‑AATGAACGCGTGCCCCTTG‑AC‑AAGAATAAAAAATTTTC‑AA‑CCCTT‑TTATCTACTCGATTTTGGT‑TTACATCCAAAAAAA‑CC‑AGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCa                                                                                                         <  2:782664/207‑1 (MQ=255)                                                                                                                                     ctcctTGAGCATCGGG‑AGAAAT‑AATGAAAGCGTGTCCCTTG‑AC‑ACGAATACAAAATTTTC‑AA‑CACAC‑TTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAA‑CC‑AGGTCAcc                                                                                                                                                                                                     >  1:208165/1‑111 (MQ=255)                                                                                                                                     ctcctTGAGCATCGGG‑AGAAAT‑AATGAAAGCGTGTCCCTTG‑AC‑ACGAATACAAAATTTTC‑AA‑CACAC‑TTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAA‑CC‑AGGTCACCGCCATTAAAATAg                                                                                                                                                                                        >  1:1969858/1‑124 (MQ=255)                                                                                                                                            aGCATCGGG‑AGAAAT‑AATGAAAGCGTGTCCCTTG‑AC‑ACGAATACAAAATTTTC‑AA‑CACAC‑TTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAA‑CC‑AGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATAtt                                                                            >  1:1012853/1‑225 (MQ=255)                                                                                                                                             acaacaggaaaaaaa‑aaTAAAAGTGTGCCCCTAC‑AC‑AC‑AAAAAAAAATTTCCAAA‑CACAT‑TTATCAACTC‑AATTTGGCTTTCCACCCAAAAAAA‑CG‑AGTCCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTg                                                                        <  2:373080/218‑1 (MQ=255)                                                                                                                                                taggg‑AAAAAT‑AATGAAGGCGTGTCCCTTG‑AC‑ACAAATAAAAAATTTTC‑AA‑CCCAT‑TTATCTACTCGATTTTGGC‑TTGCACCCGAAAAAA‑CC‑AGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGt                                                                                                                   <  2:343444/180‑1 (MQ=255)                                                                                                                                                tCGGG‑AGAAAT‑AATGAAAGCGTGTCCCTTG‑AC‑ACGAATACAAAATTTTC‑AA‑CACAC‑TTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAA‑CC‑AGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGt                                                                                                                   >  1:344066/1‑182 (MQ=255)                                                                                                                                                 cGGG‑CGAAAT‑AATGAAAGCGTGTCCCTTG‑AC‑ACGAATACAAAATTTTC‑AA‑CACAC‑TTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAA‑CC‑AGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTa                                                                           >  1:1501746/1‑221 (MQ=255)                                                                                                                                                 cGGG‑AGAAAT‑AATGAAAGCGTGTCCCTTG‑AC‑ACGAATACAAAATTTTC‑AA‑CACAC‑TTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAA‑cc                                                                                                                                                                                                              <  1:1572340/91‑1 (MQ=255)                                                                                                                                                 cGGG‑AGAAAT‑AATGAAAGCGTGTCCCTTG‑AC‑ACGAATACAAAATTTTC‑AA‑CACAC‑TTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAA‑cc                                                                                                                                                                                                              >  2:1569643/1‑91 (MQ=255)                                                                                                                                                 cGGG‑AGAAAT‑AATGAAAGCGTGTCCCTTG‑AC‑ACGAATACAAAATTTTC‑AA‑CACAC‑TTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAA‑CC‑AGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGaa                                                                                                                                                            >  1:1886589/1‑140 (MQ=255)                                                                                                                                                 cGGG‑AGAAAT‑AATGAAAGCGTGTCCCTTG‑AC‑ACGAATACAAAATTTTC‑AA‑CACAC‑TTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAA‑CC‑AGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATga                                                                                                                   >  1:755049/1‑180 (MQ=255)                                                                                                                                                 cGGG‑AGAAAT‑AATGAAAGCGTGTCCCTTG‑AC‑ACGAATACAAAATTTTC‑AA‑CACAC‑TTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAA‑CC‑AGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATAtt                                                                                         >  1:403075/1‑207 (MQ=255)                                                                                                                                                 cGGG‑AGAAAT‑AATGAAAGCGTGTCCCTTG‑AC‑ACGAATACAAAATTTTC‑AA‑CACAC‑TTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAA‑CC‑AGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTg                                                                        >  1:373754/1‑224 (MQ=255)                                                                                                                                                  gggaaaaaaT‑AAAGAAGGCGTGT‑CCTTG‑ACAAAAAAAAAAAAATTTCC‑AA‑CCCCA‑TTTCCTCCACAATTTTGGT‑TGCCCTCCAAAAAAA‑CC‑AGGTAACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATAtt                                                                                         <  2:402350/201‑1 (MQ=255)                                                                                                                                                  ggg‑AGAAAT‑AATGAAAGCGTGTCCCTTG‑AC‑ACGAATACAAAATTTTC‑AA‑CACAC‑TTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAA‑CC‑AGGTCACCGCCATTAAAATAGCGATAtt                                                                                                                                                                                 >  1:1291507/1‑118 (MQ=255)                                                                                                                                                  agaaaaaaaT‑AATGAAAACGCGTCTATAA‑CA‑ACGAAAAAAAAATTTCA‑AC‑CACAC‑TTACCATTACGATGTTGAC‑TTGCATCGAAAAAAA‑CC‑AGGACACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTa                                                                           <  2:1499153/215‑1 (MQ=255)                                                                                                                                                                   aGCGTGTCCCTTG‑AC‑ACGAATACAAAATTTTC‑AA‑CACAC‑TTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAA‑CC‑AGGTCACCGCCATTAAAATAGCGATATTCACCAt                                                                                                                                                                           >  1:641591/1‑109 (MQ=255)                                                                                                                                                                         tCCCTTG‑AC‑ACGAATACAAAATTTTC‑AA‑CACAC‑TTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAA‑CC‑AGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCtt                                             >  1:799840/1‑229 (MQ=255)                                                                                                                                                                                       aaTACAAAATTTTC‑AA‑CACAC‑TTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAA‑CC‑AGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACt                                                                                                >  1:306929/1‑166 (MQ=255)                                                                                                                                                                                                                      cTCGAATTTGGC‑TTGCATCCGCAAAAA‑CC‑AGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCAcgccg                                                    >  1:61707/1‑182 (MQ=255)                                                                                                                                                                                                                       tCGAATTTGGC‑TTGCATCCGCAAAAA‑CC‑AGGTCACCGc                                                                                                                                                                                                   >  1:1151148/1‑38 (MQ=255)                                                                                                                                                                                                                       tCGAATTTGGC‑TTGCATCCGCAAAAA‑CC‑AGGTCACCGCCATTAAAATAGCGATAtt                                                                                                                                                                                 >  1:508363/1‑56 (MQ=255)                                                                                                                                                                                                                       tCGAATTTGGC‑TTGCATCCGCAAAAA‑CC‑AGGTCACCGCCATTAAAATAGCGATATTCAcc                                                                                                                                                                             >  2:1963044/1‑60 (MQ=255)                                                                                                                                                                                                                       tCGAATTTGGC‑TTGCATCCGCAAAAA‑CC‑AGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAGTCAAATTGATTGATAAATAAATTTAAACGAAGCGGgag    >  2:1642100/1‑229 (MQ=255)                                                                                                                                                                                                                       tCGAATTTGGC‑TTGCATCCGCAAAAA‑CC‑AGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAGTCAAATTGATTGATAAATAAATTTAAACGAAGCGGgag    >  2:1292254/1‑229 (MQ=255)                                                                                                                                                                                                                       tCGAATTTGGC‑TTGCATCCGCAAAAA‑CC‑AGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAGTCAAATTAATTGATAAATTAATTTAAACGAAACGGgag    >  2:1071465/1‑229 (MQ=255)                                                                                                                                                                                                                         gattttGTC‑TTGCACCAAAAAAAA‑CA‑AGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATa                                                                                                                                         <  2:1161796/91‑1 (MQ=255)                                                                                                                                                                                                                         gattttGGC‑TTGCACCAGAAAAAC‑CC‑AGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGt                                                                                                     <  2:552402/127‑1 (MQ=255)                                                                                                                                                                                                                         gAATTTGGC‑TTGCATCCGCAAAAA‑CC‑AGGTCACCGCCATTAAAATAGCGATAtt                                                                                                                                                                                 >  1:1804953/1‑54 (MQ=255)                                                                                                                                                                                                                         gAATTTGGC‑TTGCATCCGCAAAAA‑CC‑AGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATa                                                                                                                                         >  1:1163824/1‑94 (MQ=255)                                                                                                                                                                                                                         gAATTTGGC‑TTGCATCCGCAAAAA‑CC‑AGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGt                                                                                                     >  1:1321630/1‑130 (MQ=255)                                                                                                                                                                                                                         gAATTTGGC‑TTGCATCCGCAAAAA‑CC‑AGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGt                                                                                                     >  1:553375/1‑130 (MQ=255)                                                                                                                                                                                                                          gattttgcatcgCACCCACAAAAA‑CC‑AGACTCCCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAGTCAAATTGATTGATAAATAAATTTAAACGAAGCGGGAGaa  <  2:1820355/218‑1 (MQ=255)                                                                                                                                                                                                                                gC‑TTGCCCCCGAAAAAC‑CC‑GGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTccc                                                                                                                       <  2:1422647/105‑1 (MQ=255)                                                                                                                                                                                                                                gC‑TTGCATCCGCAAAAA‑CC‑AGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTccc                                                                                                                       >  1:1425091/1‑105 (MQ=255)                                                                                                                                                                                                                                      cATCCGCAAAAA‑CC‑AGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGt                                                                                                                >  2:1428717/1‑107 (MQ=255)                                                                                                                                                                                                                                      cATCCGAAAAAA‑CC‑AGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGt                                                                                                                <  1:1431171/107‑1 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                     AGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATG‑TGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGG‑AGAAAT‑AATGAAAGCGTGTCCCTTG‑AC‑ACGAATACAAAATTTTC‑AA‑CACAC‑TTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAA‑CC‑AGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAGTCAAATTGATTGATAAATAAATTTAAACGAAGCGGGAGAA  >  NC_000913/3665476‑3665912

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑