Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,665,702	0	C	A	38.6%	131.7 / 38.1	70	A37S (GCG→TCG)	gadX	acid resistance regulon transcriptional activator; autoactivator
Reads supporting (aligned to +/- strand):  ref base C (38/5);  new base A (0/27);  total (38/32)
Fisher's exact test for biased strand distribution p-value = 1.11e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTAC‑AGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATG‑CCTCCTCCTTGA‑GCATC‑GGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTC‑AACACACTTATCTACTCGAATTTGG‑CTTGCATCCGCAAAAA‑CCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAGTCAAATTGATTGATAAATAAATTTAAACGAAGCGGGAGAACAGGATGATAATGTGTGAT  >  NC_000913/3665468‑3665931                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                        ttttACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTAC‑AGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATG‑CCTCCTCCTTGA‑GCATC‑GGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTC‑AACACACTTATCTACTCGAATTTGG‑CTTGCATCCGCaaaa                                                                                                                                                                                                                                     >  1:1532822/1‑239 (MQ=255)   ttACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTAC‑AGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATG‑CCTCCTCCTTGA‑GCATC‑GGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTC‑AACACACTTATCTACTCGAATTTGG‑CTTGCATCCGCAAAAA‑c                                                                                                                                                                                                                                  <  1:1958792/239‑1 (MQ=255)           cATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTAC‑AGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATG‑CCTCCTCCTTGA‑GCATC‑GGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAACTTTTC‑AACACACTTATCTCCTCGAATTTGG‑CTTGCATCCGCAAAAA‑CCCGGT‑cc                                                                                                                                                                                                                          >  1:890698/1‑237 (MQ=255)           cATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTAC‑AGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATG‑CCTCCTCCTTGA‑GCATC‑GGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTC‑AACACACTTATCTACTCGAATTTGG‑CTTGCATCCGCAAAAA‑cc                                                                                                                                                                                                                                 <  2:1657980/232‑1 (MQ=255)           cATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTAC‑AGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATG‑CCTCCTCCTTGA‑GCATC‑GGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTC‑AACACACTTATCTACTCGAATTTGG‑CTTGCATCCGCAAAAA‑cc                                                                                                                                                                                                                                 >  1:1657980/1‑232 (MQ=255)           cATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTAC‑AGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATG‑CCTCCTCCTTGA‑GCATC‑GGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTC‑AACACACTTATCTACTCGAATTTGG‑CTTGCATCCGCAAAAA‑CCAGGT‑Ca                                                                                                                                                                                                                          >  2:789041/1‑238 (MQ=255)                     tAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTAC‑AGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATG‑CCTCCTCCTTGA‑GCATC‑GGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTC‑AACACACTTATCTACTCGAATTTGG‑CTTGCATCCGCAAAAA‑CCAGGT‑CACCGCCATTaaa                                                                                                                                                                                                               >  1:2095115/1‑239 (MQ=255)                      aGCGAAGTTTTTTATTCTGCGTTGGTGGCGCAACTTCCTAAAAAACAGGAATATCTCTACTAC‑AGTGATGAACGAGTAATGAAGAAATTTTTTTATGTGCATGAAGTTTTTATG‑CCTCCTCCTTGG‑GCATC‑GGGAGAAATAATGAAAGCGTGTCCCTTGACTCGAATACAAAATTTTC‑AACACACTTTTATACTCAATTTTGG‑CTTCCCTCCGAAAAAA‑CCAGGT‑CACCGCCTTTaaaa                                                                                                                                                                                                              <  2:857325/239‑1 (MQ=255)                                 tttttttgagATATTTGCACATTTTCCAAAAAAAAAAGAATATCTCTTATAA‑AGGGATGAACGAGTAAAAAAAAATCTTTTTTATGTGAATAAAGATTTAATC‑CCCCCTCCTTGG‑GCAGC‑GGGAGAAATAATGAAGGCGTGTCCTTTTACACAAATAAAAATTTTTC‑AACACTTTTATCTTC‑AAAATTTGGTTTTCCACCCAAAAAAA‑CCAGTT‑CACCGCCATTAAAATAGCGATATTc                                                                                                                                                                                                   <  2:1532822/230‑1 (MQ=255)                                      cggtaaTAGTTCCCAAATTTCCAAAAAAACATAAATTTCTC‑ACCACAAGTGATAAACGATAAAAAAAGAAATTTTTTTATGTGCATGAAGTTTTAACC‑CCCCCCCCTTAG‑ACATC‑GGAAAAAAAAAGAAAGGGGTCCCCCTTAACACAAAAAAAAAATTTTC‑AACCCTTTTATC‑ACCCAAATTTGG‑CTTTCCCCCCAAAAAACCCGGGC‑CACCGCCATTAAAATAGCGATATTCACCAt                                                                                                                                                                                              <  2:296467/234‑1 (MQ=255)                                       tGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTAC‑AGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATG‑CCTCCTCCTTGA‑GCATC‑GGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTC‑AACACACTTATCTACTCGAATTTGG‑CTTGCATCCGCAAAAA‑CCAGGT‑CACCGCCAtt                                                                                                                                                                                                                  >  1:1982942/1‑218 (MQ=255)                                       tGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTAC‑AGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATG‑CCTCCTCCTTGA‑GCATC‑GGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTC‑AACACACTTATCTACTCGAATTTGG‑CTTGCATCCGCAAAAA‑CCAGGT‑CACCGCCATTAAAATAGCGATATTCACCAtt                                                                                                                                                                                             >  2:424636/1‑239 (MQ=255)                                         cGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTAC‑AGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATG‑CCTCCTCCTTGA‑GCATC‑GGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTC‑AACACACTTATCTACTCGAATTTGG‑CTTGCATCCGCAAAAA‑CCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTa                                                                                                                                                                                            >  1:472308/1‑238 (MQ=255)                                           atggtgcagaaaCTTCCAAAAAAAAAGAAATTTCTCAAATAC‑AGAAAGAAACGAAAAAAAAAAAATTTTTTTTATGGGAAAAAAATTTTAATC‑CCCCCCCATTGA‑GACTG‑GGAAAAAAAAATAAAAGCGTGCCCTTAGAAAAAAATAAAAAATTTCA‑AACACATTTATCT‑CCAAAATTTGG‑GTTTCACCCCAAAAAACCCGGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAAc                                                                                                                                                                                          <  2:1031447/228‑1 (MQ=255)                                                     aactcctaaaaaaaCGGAAATGTCTTTCCTAC‑AGTGATAAACGATTAATAAATAATTTTTTTTATGTGCATGAAGTTTTATTC‑CCTCCTCCTTGA‑ACATC‑GGGAAAAATAATAAAAGCGTCTCCTTTGACACGAATACAAATTTTCC‑ACCACTTTTTTCTCCTC‑AATTTGGTCTTGCACCCGCAAAAA‑CGGGTT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGaa                                                                                                                                                                               <  2:842336/230‑1 (MQ=255)                                                     aaCTTCCTAAAAAAAAGAAATTTCTCTCCTTC‑AGGAATGAACGAGTAATGAAGAATCTTTTTTATGTGCATGAAGTTTTAATC‑CCCCCTCTTGAA‑GCAAG‑GGGAAAAATAATGAAAGCTTGCCACTTGACACAAAAAAAAATTTATC‑AACCCACTTATCTGCAAAATTTTGG‑TTGACACCCAAAAAAA‑CAAGGA‑AACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGaa                                                                                                                                                                               <  2:1321682/239‑1 (MQ=255)                                                          ccTGAAAAACAGGAATATCTCTACTAC‑AGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATG‑CCTCCTCCTTGA‑GCATC‑GGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTC‑AACACACTTATCTACTCGAATTTGG‑CTTGCATCCGCAAAAA‑CCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAatatat                                                                                                                                                                          >  1:1466790/1‑239 (MQ=255)                                                                        atatCTTTATTAA‑AGGAATGAAGGAATAATGAAAATTTTTTTTTAGGTAAATGAAAATTT‑ATG‑CCCCCCCCATGG‑AGATCGGGGAAAAATAAGGAAGCGGTGGCGCGAGAAGAGAATAAAAAATTTCC‑AACCAACTATTCTACTAAATTTTGT‑CTTCCACCAGAAAAAA‑CAAATA‑ACCCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATa                                                                                                                                                            <  2:2095115/239‑1 (MQ=255)                                                                        atatCTTTACTAA‑AGTGAGGAAGGAGTAATAAAAAATTTTTTTTTTGGACAGAAAGATAAAATC‑CCCCCTCTTGGA‑GACGG‑GGGAAAAATAATGAAGGCGTGCCCATTGAAAAAAAAAAAAAATTTAC‑AACACATTTATCTACCAGATTTGGT‑TTTGCACACGAAAAAA‑CCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATa                                                                                                                                                            <  2:1466790/239‑1 (MQ=255)                                                                        atatCTCTACTAA‑AGTGATGAACAAGTAATGAAGAATTTTTTTTATGTGCATGAAGATTTAAGG‑CCTCCTCCTTGA‑GCATC‑GGGAAAAATAATGAAGGCTTGTCCTTTGACAAAAAAAAAAAATTTTC‑AACACACTTATCTACTCGAATTTGG‑CTTGCACCCAAAAAAA‑CCGGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATa                                                                                                                                                            <  2:450889/239‑1 (MQ=255)                                                                        atatCTCTAATAA‑AGAGAAGAACAAATAAAAAAAAATTTTTTTTATGTGAAAGAAGATTTAATG‑CCCCCTCCTTGA‑GCATC‑GGAAAAAAAAAAGAAGGCTTGCCCTTTAACACAAAAAAAAAATTTTA‑ACCACTTTTATCTCCTAAAATTTGG‑TTACCACCCAAAAAAA‑CCAGGT‑CCCCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATa                                                                                                                                                            <  2:874973/239‑1 (MQ=255)                                                                                actac‑AGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATG‑CCTCCTCCTTGA‑GCATC‑GGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTC‑AACACACTTATCTACTCGAATTTGG‑CTTGCATCCGCAAAAA‑CCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGCGAATATATTTATGTATTGCATACGCAATTa                                                                                                                                                    >  2:68742/1‑239 (MQ=255)                                                                                    gaaGTAATGAAGGAGTAAGAAAAAAGTTTTTTTATGGACATGAATTTTAAATG‑CCCCCTCCTTGA‑GAATC‑GGGAAAAAAAATGAAAGCGTTCCCTTTGACACAAATAAAAATTTTAC‑AACACTTTTATCTACTTAATTTTGG‑CTTGCACCCGAAAAAC‑CCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGAc                                                                                                                                                 <  2:611250/237‑1 (MQ=255)                                                                                      tgtgataacggAGTAATGAATAAATTTTTTTAAGTGCAAAAAATTTGAACA‑CCCCTCCCCTGG‑GTCTG‑GGGAAAAAAAATTAAAGCTTCTCAATTAAACCAAAAAAAAAATTTCC‑AACACTTTTATTTCCTGAAATTTGA‑AAGCAACCCGCAAAAA‑CCAGGC‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGa                                                                                                                                                  <  2:1613982/226‑1 (MQ=255)                                                                                       gTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATG‑CCTCCTCCTTGA‑GCATC‑GGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTC‑AACACACTTATCTACTCGAATTTGG‑CTTGCATCCGCAAAAA‑CCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAAt                                                                                                                                              >  1:501787/1‑239 (MQ=255)                                                                                        tgatgaACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATG‑CCTCCTCCTTGA‑GCATC‑GGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTC‑AACACACTTATCTACTCGAATTTGG‑CTTGCATCCGCAAAAA‑CCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAAt                                                                                                                                              >  1:2141876/1‑238 (MQ=255)                                                                                        tgatgaACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATG‑CCTCCTCCTTGA‑GCATC‑GGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTC‑AACACACTTATCTACTCGAATTTGG‑CTTGCATCCGCAAAAA‑CCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAAt                                                                                                                                              >  1:1613982/1‑238 (MQ=255)                                                                                          atgaACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATG‑CCTCCTCCTTGA‑GCATC‑GGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTC‑AACACACTTATCTACTCGAATTTGG‑CTTGCATCCGCAAAAA‑CCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTc                                                                                                                                            >  1:339607/1‑238 (MQ=255)                                                                                          ataacggagaAATAAAAAAACTTTTTTATGGGTAAAAAATTTAAACC‑CCTCCTCCTTGA‑GCATG‑GGAAAAAAAAAAAAACGCGTGTCCCTTAAAAAAAAAAAAAAATTTCA‑ACCACCTTTATCTATTC‑AATTTGGTCTTCCACCCCAAAAAA‑CCAGGT‑AACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACaa                                                                                                                                               <  2:1831941/229‑1 (MQ=255)                                                                                             aaCGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATG‑CCTCCTCCTTGA‑GCATC‑GGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTC‑AACACACTTATCTACTCGAATTTGG‑CTTGCATCCGCAAAAA‑CCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCAt                                                                                                                                        >  1:611250/1‑239 (MQ=255)                                                                                                  gTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATG‑CCTCCTCCTTGA‑GCATC‑GGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTC‑AACACACTTATCTACTCGAATTTGG‑CTTGCATCCGCAAAAA‑CCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGt                                                                                                                                   >  1:1516337/1‑239 (MQ=255)                                                                                                  gTAATGAAAATATTTTTTTATGTGCAGGAAGTTTTAATG‑CCGCCTCCTTGA‑GCTGC‑GGGAGAAATAATGAACGCGTGTCCCTTCACAAGAAAAAAAATTTTTC‑AACACACTTATCTACTCGATTTTGT‑CTTGCCTCCGAAAAAC‑CCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAg                                                                                                                                    <  2:297155/238‑1 (MQ=255)                                                                                                    aataaaaaagtttttttATGTGCAT‑AAAATTTAAAGCCCCCCTCCTTGA‑GCATC‑GGAAAAAAAAATGAATGCGTGCCCTTTAACAAAAAAAAAAATTTTTC‑AACCCTCTTATCTCCCCGATTTTGG‑CTTCCACCCAAAAAAC‑CCGGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGt                                                                                                                                   <  2:266427/226‑1 (MQ=255)                                                                                                    aaTGAAGAAACTTTTTTATGTGCATGAAGATTTAATG‑CCTCCTCCTTGA‑GCATC‑GGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTC‑AACACACTTATCTACTCGAATTTGG‑CTTGCATCCGCAAAAA‑CCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTa                                                                                                                                     >  1:1831941/1‑235 (MQ=255)                                                                                                                  tttagttgaaagAAGATTTAATG‑CCTCCTCGTTGA‑GCATG‑GGAAAAAATAATAAAGGCGTGCCCCTTCACACAAATAAAAAATTTCC‑AACCCTCTTATCACCTCGATTTTGG‑TTTCCCCCCAAAAAAC‑CCAGTT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACt                                                                                                                   <  2:1516337/228‑1 (MQ=255)                                                                                                                             aagtaTTTAATC‑CCTCCT‑CTTGAGGGAGC‑GGGAAAAAAAATGAAGGCGGTTCCTTTAACAAGAAAAAAAAATTTTC‑AACCACCTTATCTCCTGGAATTTGG‑TTTACATCAGAAAAAA‑CGAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACAt                                                                                                        <  2:291601/235‑1 (MQ=255)                                                                                                                               aattttAACC‑CCCCCTCCTTGA‑GCAGG‑GGAAAAAATAATGAAAGCGTTTCCCTTGACAAAAAAAAAAAATTTCA‑AACCCACTTATCTACTCAATTTTGG‑CTTCCATCCAAAAAAA‑CCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACAt                                                                                                        <  2:339607/234‑1 (MQ=255)                                                                                                                               aGATTTAATC‑CCCCCTCCTGGA‑GAGAG‑GGAAAAAATAATGATAGCGTCTCCCTTAACAAAAATAAAAAATTCAC‑AACACTTTTATCTACTCAATTTGGG‑TTTGCACCCAAAAAAC‑CCGGAC‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACAt                                                                                                        <  2:2105243/237‑1 (MQ=255)                                                                                                                                         ccctctccctAGA‑GCAGC‑GGAAAAAAAAATAAACGCTTGTCCTTTAACACAAAAAAAAATTTTTC‑AACACATTTATCTACTAAATTTTGG‑CTTGCACCCGAAAAAC‑CCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTa                                                                                              <  2:881885/231‑1 (MQ=255)                                                                                                                                          cctccccctGGA‑GCAGG‑GGGAAAAAAAAAGAAGGCGTGCCCTTTAACACAAATA‑AAAATTTTCAAACACTTTTTTCTACTCGATTTGGT‑CTTGCA‑CCCAAAAAA‑CCAAGTCCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACt                                                                                            <  2:501787/239‑1 (MQ=255)                                                                                                                                          cccccccctAGA‑CCATC‑GGGAAAAATAATGAAGGCGTCTCCCTCGAAACAAATAAAAATTTTTA‑AACACACTTATCTACCCAATTTTGT‑CTTGCTCCCAAAAAAC‑CCAGGT‑CTCCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACt                                                                                            <  2:2216871/233‑1 (MQ=255)                                                                                                                                            tcctcctTGA‑GCATC‑GGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTC‑AACACACTTATCTACTCGAATTTGG‑CTTGCATCCGCAACAA‑CCAGGC‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAatatat                                                                                                                                                                          >  1:2040806/1‑159 (MQ=255)                                                                                                                                            tcctcctTGA‑GCATC‑GGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTC‑AACACACTTATCTACTCGAATTTGG‑CTTGCATCCGCAAAAA‑CCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATAtt                                                                                               >  1:175369/1‑234 (MQ=255)                                                                                                                                            tcctcctTGA‑GCATC‑GGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTC‑AACACACTTATCTACTCGAATTTGG‑CTTGCATCCGCAAAAA‑CCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATAtt                                                                                               >  1:2101181/1‑234 (MQ=255)                                                                                                                                              ctcctTGA‑GCATC‑GGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTC‑AACACACTTATCTACTCGAATTTGG‑CTTGCATCCGCAAAAA‑CCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATAtt                                                                                               >  1:1135986/1‑232 (MQ=255)                                                                                                                                                cctTGA‑GCAGG‑GGAAAAAAAAAAGAAAGCGTGCCCCTTGACAAAAAAAAAAATTTTTC‑AACACACTTATCTACTCAATTTTGG‑CTTGCACCCAAAAAAA‑CCAGGC‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAata                                                                                                                                                                             <  2:1403021/152‑1 (MQ=255)                                                                                                                                                   tGA‑GCATC‑GGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTC‑AACACACTTATCTACTCGAATTTGG‑CTTGCATCCGCAAAAA‑CCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTttatta                                                                                    >  1:881885/1‑238 (MQ=255)                                                                                                                                                           c‑GGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTC‑AACACACTTATCTACTCGAATTTGG‑CTTGCATCCGCAAAAA‑CCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGc                                                                             >  2:607085/1‑238 (MQ=255)                                                                                                                                                           c‑GGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTC‑AACACACTTATCTACTCGAATTTGG‑CTTGCATCCGCAAAAA‑CCAGGC‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTc                                                                                                                                                                   >  1:1403021/1‑152 (MQ=255)                                                                                                                                                           c‑GGGAGAAATAATGAAAGCGCGTCCCTTGACACGAATACAAAATTTTC‑AACACACTTATCTACTCGAATTTGG‑CTTGCATCCGCAAAAA‑CCAGGT‑CACCGCCATTAAAATAGCGATATTTACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGc                                                                             >  2:355365/1‑238 (MQ=255)                                                                                                                                                           c‑GGGAGAAATAATGAAAGAGTGTCCCTTGACACGAATACAAAATTTTC‑AACACACTTATCTACTCGAATTTGG‑CTTGCATCCGCAAAAA‑CCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCTCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGc                                                                             >  2:200690/1‑238 (MQ=255)                                                                                                                                                               aaaaaaaaaTAAAAGCGTGTCCTTTGACAAGAAAAAAAATTTTAC‑ACCACCTTTTTCTACTAAATTTTGC‑TTTCCCCCCAAAAAAA‑CCAGGT‑CACCGCCATTAAAATAGCGATATTCa                                                                                                                                                                                                  <  2:1759330/115‑1 (MQ=255)                                                                                                                                                                 aaaaaaaTGAAGGCGTCTCCCTGCACA‑AAAAAAAAAATTTTCAAACCCCTTTTTCTACTAAATTTTGG‑CTTCCACCAAAAAAAA‑CAAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCAcgccg                                                                       <  2:472308/238‑1 (MQ=255)                                                                                                                                                                  aaaTAATGAAAGCGTCTCCATTGACAAAAATAAAAATTTTTC‑AACACACTTATCTACTCAATTTTGG‑CTTGCATCCGAAAAAC‑CCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCAcgccgc                                                                      <  1:1725138/239‑1 (MQ=255)                                                                                                                                                                   aataatGAAAGCGTGTCCCTTGACACGAAAACAAAATTTCC‑AACACTCTTATCTACTCGAATTTGG‑CTTGCATCCGCAAAAA‑CCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACgccgcc                                                                     <  1:1330827/239‑1 (MQ=255)                                                                                                                                                                         gAAAGCGTGTCCCTTGACACGAATACAAAATTTTC‑AACACACTTATCTACTCGAATTTGG‑CTTGCATCCGCAAAAA‑CCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCa                                                                                                                                                                                        >  1:1759330/1‑118 (MQ=255)                                                                                                                                                                            aGCGTGTCCCTTGACACGAATACAAAATTTTC‑AACACACTTATCTACTCGAATTTGG‑CTTGCATCCGCAAAAA‑CCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAg                                                             >  1:282918/1‑238 (MQ=255)                                                                                                                                                                             gCGTGTCCCTTGACACGAATACAAAATTTTC‑AACACACTTATCTACTCGAATTTGG‑CTTGCATCCGCAAAAA‑CCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAGTc                                                           >  1:2228314/1‑239 (MQ=255)                                                                                                                                                                                              aaTACAAAATTTTC‑AACACACTTATCTACTCGAATTTGG‑CTTGCATCCGCAAAAA‑CCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAGTCAAATTGATTGataaata                                          >  1:1950387/1‑239 (MQ=255)                                                                                                                                                                                              aaTACAAAATTTTC‑AACACACTTATCTACTCGAATTTGG‑CTTGCATCCGCAAAAA‑CCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAGTCAAATTGATTGataaata                                          >  1:1445775/1‑239 (MQ=255)                                                                                                                                                                                               aTACAAAATTTTC‑AACACACTTATCTACTCGAATTTGG‑CTTGCATCCGCAAAAA‑CCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAGTCAAATTGATTGataaataa                                         >  1:1188264/1‑239 (MQ=255)                                                                                                                                                                                                                  tattaccgaCTAGAATTGGG‑TTTGCATCAGAAAAAA‑CAGGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAAGATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAGTCAAATTGATTGATAAATAAATTTAAACGAAGCGGgag                       <  2:1852719/231‑1 (MQ=255)                                                                                                                                                                                                                    ttATCTACTCGATTTTGG‑TTTGCACCCAAAAAAC‑CCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAGTCAAATTGATTGATAAATAAATTTAAACGAAGCGGGAGaa                     <  1:810804/239‑1 (MQ=255)                                                                                                                                                                                                                            tCGAATTTGG‑CTTGCATCCGCAAAAA‑CCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAatatat                                                                                                                                                                          >  2:226779/1‑82 (MQ=255)                                                                                                                                                                                                                            tCGAATTTGG‑CTTGCATCCGCAAAAA‑CCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAGTCAAATTGATTGATAAATAAATTTAAACGAAGCGGGAGAACAGgatga             >  1:1584122/1‑239 (MQ=255)                                                                                                                                                                                                                              gAATTTGG‑CTTGCATCCGCAAAAA‑CCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATa                                                                                                                          >  1:1860636/1‑128 (MQ=255)                                                                                                                                                                                                                                aTTTGG‑CTTGCATCCGCAAAAA‑CCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAGTCAAATTGATTGATAAATAAATTTAAACGAAGCGGGAGAACAGGATGATAAt         >  2:1603737/1‑239 (MQ=255)                                                                                                                                                                                                                                   tGG‑CTTGCATCCGCAAAAA‑CCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAAc                                                                                                                                                                                          >  1:992341/1‑59 (MQ=255)                                                                                                                                                                                                                                        ttGCACCCGAAAACA‑CAGGGT‑AACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAGTCAAATTGATTGATAAATAAATTTAAACGAAGCGGGAGAACAGGATGATAATGTGTGAt  <  2:1584122/239‑1 (MQ=255)                                                                                                                                                                                                                                           cATCCGCAAAAA‑CCAGGT‑CACCGCCATTAAAATAGCGATATTCACCa                                                                                                                                                                                               >  1:2095136/1‑47 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                        TTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTAC‑AGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATG‑CCTCCTCCTTGA‑GCATC‑GGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTC‑AACACACTTATCTACTCGAATTTGG‑CTTGCATCCGCAAAAA‑CCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAGTCAAATTGATTGATAAATAAATTTAAACGAAGCGGGAGAACAGGATGATAATGTGTGAT  >  NC_000913/3665468‑3665931

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑