| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 884,184 | 0 | A | G | 33.3% | 107.3 / 10.6 | 75 | E171G (GAG→GGG) | mdfA | multidrug efflux system protein |
| Reads supporting (aligned to +/- strand): ref base A (20/30); new base G (19/6); total (39/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.75e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TTATCGTCACCTGTCTGGCAATATTGCTGGCGCAAAACATTGAACAATTCACCCTGTTGCGCTTCTTGCAGGGCATAAGCCTCTGTTTCATTGGCGCTGTGGGATACGCCGCAATTCAGGAATCCTTCGAAGAGGCGGTTTGTATCAAGATCACCGCGCTGATGGCGAACGTGGCGCTGATTGCTCCGCTACTTGGTCCGCTGGTGGGCGCGGCGTGGATCCATGTGCTGCCCTGGGAGGGGATGTTTGTT‑TTGTTTGCCGCAT‑TGGCAGCGATCTCCTTTTTCGGTCTG‑CAACGAGCCATGCCTGAAACCGCCACGCGTATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCTGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGGATCGCCCAGTCGCC > NC_000913/883947‑884400 | ttATCGTCACCTGTCTGGCAATATTGCTGGCGCAAAACATTGAACAATTCGCCCTGTTGCGCTTCTTGCAGGGCATAAGCCTCTGTTTCATTGGCGCTGTGGGATACGCCGCAATTCAGGAATCCTTCGAAGAGGCGGTTTGTATCAAGATCACCGCGCTGATGGCGAACGTGGCGCTGATTGCTCCGCTACTTGGTCCGCTGGTGGGCGCGGCGTGGATCCATGTGCTGCCCTGGGa < 1:1599857/238‑1 (MQ=255)ttATCGTCACCTGTCTGGCAATATTGCTGGCGCAAAACATTGAACAATTCACCCTGTTGCGCTTCTTGCAGGGCATAAGCCTCTGTTTCATTGGCGCTGTGGGATACGCCGCAATTCAGGAATCCTTCGAAGAGGCGGTTTGTATCAAGATCGCCGCGCTGATGGCGAACGTGGCGCTGATTGCTCCGCTACTTGGTCCGCTGGTGGGCGCGGCGTGGATCCATGTGCTGCCCTGGGAg < 1:993318/239‑1 (MQ=255) aCCTGTCTGGCAATATTGCTGGCGCAAAACATTGAACAATTCACCCTGTTGCGCTTCTTGCAGGGCATAAGCCTCTGTTTCATTGGCGCTGTGGGATACGCCGCAATTCAGGAATCCTTCGAAGAGGCGGTTTGTATCAAGATCACCGCGCTGATGGCGAACGTGGCGCTGATTGCTCCGCTACTTGGTCCGCTGGTGGGCGCGGCGTGGATCCATGTGCTGCCCTGGGGGGGGAggtt > 1:1681482/1‑239 (MQ=255) aCCTGTCTGGCAATATTGCTGGCGCAAAACATTGAACAATTCACCCTGTTGCGCTTCTTGCAGGGCATAAGCCTCTGTTTCATTGGCGCTGTGGGATACGCCGCAATTCAGGAATCCTTCGAAGAGGCGGTTTGTATCAAGATCACCGCGCTGATGGCGAACGTGGCGCTGATTGCTCCGCTACTTGGTCCGCTGGGGGGCGCGGCGTGGATCCATGTGCTGCCCTGGGAGGGGAtgtt > 1:1356989/1‑239 (MQ=255) atatTGCTGGCGCAAAACATTGAACAATTCACCCTGTTGCGCTTCTTGCAGGGCATAAGCCTCTGTTTCATTGGCGCTGTGGGATACGCCGCAATTCAGGAATCCTTCGAAGAGGCGGTTTGTATCAAGATCACCGCGCTGATGGCGAACGTGGCGCTGATTGCTCCGCTACTTGGTCCGCTGGTGGGCGCGGCGTGGATCCCTGTGCTGGCCTGGGGGGGGGGGTTTGGt‑ttgtttg > 1:1419376/1‑238 (MQ=255) atatTGCTGGCGCAAAACATTGAACAATTCACCCTGTTGCGCTTCTTGCAGGGCATAAGCCTCTGTTTCATTGGCGCTGTGGGATACGCCGCAATTCAGGAATCCTTCGAAGAGGCGGTTTGTATCAAGATCACCGCGCTGATGGCGAACGTGGCGCTGATTGCTCCGCTACTTGGTCCGCTGGTGGGCGCGGCGTGGATCCATGTGCTGCCCTGGGAGGGGATGTTTGTT‑TTGTTTGc < 1:236594/239‑1 (MQ=255) ttGCTGGCGCAAAACATTGAACAATTCACCCTGTTGCGCTTCTTGCAGGGCATAAGCCTCTGTTTCATTGGCGCTGTGGGATACGCCGCAATTCAGGAATCCTTCGAAGAGGCGGTTTGTATCCAGATCACCGCGCTGATGGCGAACGTGGCGCTGGTTGCTCCGCTACTTGGTCCGCTGGTGGGCGAGGCGCGGAGCCATGTTCTGCCCTGGGGGGGGagggt > 2:1224427/1‑220 (MQ=255) gCTGGCGCAAAACATTGAACAATTCGCCCTTTTGCGCTTCTTGCAGGGCATAAGCCTCTGTTTCATTGGCGCTGTGGGATACGCCGCAATTCAGGAATCCTTCGAAGAGGCGGTTTGTATCAAGATCACCGCGCTGATGGCGAACGTGGCGCTGATTGCTCCGCTACTTGGTCCGCTGGTGGGCGCGGCGTGGATCCATGTGCTGCCCTGGGAGGGGATGTTTGTT‑TTGTTTGCCGCAt < 1:335721/239‑1 (MQ=255) ggCGCAAAACATTGAACAATTCACCCTGTTGCGCTTCTTGCAGGGCATAAGCCTCTGTTTCATTGGCGCTGTGGGATACGCCGCAATTCAGGAATCCTTCGAAGAGGCGGTTTGTATCAAGATCACCGCGCTGATGGCGAACGTGGCGCTGATTGCTCCGCTACTTGGTCCGCTGGTGGGCGCGGCGTGGATCCATGTGCTGCCCTGGGAGGGGATGTTTGTT‑TTGTTTGCCGCAT‑Tgg < 1:992225/239‑1 (MQ=255) aaCATTGAACAATTCACCCTGTTGCGCTTCTTGCAGGGCATAAGCCTCTGTTTCATTGGCGCTGTGGGATACGCCGCAATTCAGGAATCCTTCGAAGAGGCGGTTTGTATCAAGATCACCGCGCTGATGGCGAACGTGGCGCTGATTGCTCCGCTACTTGGTCCGCTGGTGGGCGCGGCGTGGATCCATGTGCTGCCCTGGGAGGGGATGTTTGTT‑TTGTTTGCCGCAT‑TGGCAGCGAt < 1:2060824/239‑1 (MQ=255) aCATTGAACAATTCACCCTGTTGCGCTTCTTGCAGGGCATAAGCCTCTGTTTCATTGGCGCTGTGGGATACGCCGCAATTCAGGAATCCTTCGAAGAGGCGGTTTGTATCAAGATCACCGCGCTGATGGCGAACGTGGCGCTGATTGCTCCGCTACTTGGTCCGCTGGTGGGCGCGGCGTGGATCCATGTGCTGCCCTGGGAGGGGATGTTTGTT‑TTGTTTGCCGCAT‑TGGCAGCGAtc < 1:1276809/239‑1 (MQ=255) tGAACAATTCACCCTGTTGCGCTTCTTGCAGGGCATAAGCCTCTGTTTCATTGGCGCTGTGGGATACGCCGCAATTCAGGAATCCTTCGAAGAGGCGGTTTGTATCAAGATCACCGCGCTGATGGCGAACGTGGCGCTGATTGCTCCGCTACTTGGTCCGCTGGTGGGCGCGGCGTGGATCCCTGTGCTGCCCTGGGAGGGGATGTTTGTT‑TTGTTTGCCGCAT‑TGGCa > 1:583988/1‑229 (MQ=255) aCAATTCACCCTGTTGCGCTTCTTGCAGGGCATAAGCCTCTGTTTCATTGGCGCTGTGGGATACGCCGCAATTCAGGAATCCTTCGAAGAGGCGGTTTGTATCAAGATCACCGCGCTGATGGCGAACGTGGCGCTGATTGCTCCGCTACTTGGTCCGCTGGTGGGCGCGGCGTGGATCCATGTGCTGCCCTGGGAGGGGATGTTTGTT‑TTGTTTGCCGCAT‑TGGCAGCGATCTCCtttt < 2:1682162/239‑1 (MQ=255) aTTCACCCTGTTGCGCTTCTTGCAGGGCATAAGCCTCTGTTTCATTGGCGCTGTGGGATACGCCGCAATTCAGGAATCCTTCGAAGAGGCGGTTTGTATCAAGATCACCGCGCTGATGGCGAACGTGGCGCTGATTGCTCCGCTACTTGGTCCGCTGGTGGGCGCGGCGTGGATCCATGTGCTGCCCTGGGAGGGGATGTTTGTT‑TTGTTTGCCGCAT‑TGGCAGCGATCTCCTTTTTCg < 1:2248125/239‑1 (MQ=255) ttCACCCTGTTGCGCTTCTTGCAGGGCATAAGCCTCTGTTTCATTGGCGCTGTGGGATACGCCGCAATTCAGGAATCCTTCGAAGAGGCGGTTTGTATCAAGATCACCGCGCTGATGGCGAAAGTGGCGCTGATTGCTCCGCTACTTGGTCCGCTGGTGGGCGCGGCGTGGGTCCATGGGCGGCCCTGGGGGGGGATGTTTGTT‑TTGTTTGGCGCATGTGGC‑GCGAGCTCCtttt > 2:1960769/1‑235 (MQ=255) tCACCCTGTTGCGCTTCTTGCAGGGCATAAGCCTCTGTTTCATTGGCGCTGTGGGATACGCCGCAATTCAGGAATCCTTCGAAGAGGCGGTTTGTATCAAGATCACCGCGCTGATGGCGAACGTGGCGCTGATTGCTCCGCTACTTGGTCCGCTGGTGGGCGCGGCGTGGATCCATGTGCTGCCCTGGGAGGGGATGTTTGTT‑TTGTTTGCCGCAT‑TGGCAGCGATCTCCTTTTTCGGt < 1:351218/239‑1 (MQ=255) gTTGCGCTTCTTGCAGGGCATAAGCCTCTGTTTCATTGGCGCTGTGGGATACGCCGCAATTCAGGAATCCTTCGAAGAGGCGGTTTGTATCCAGATCACCGCGCTGATGGCGAACGTGGCGCTGATTGCTCCGCTACTTGGTCCGCTGGTGGGCGCGGCGTGGATCCATGGGCTGCCCTGGGGGGGGGTGTTTGTT‑TTGTTTGGCGCAT‑TGGGAGCGGTCTCCTTTTTCGGTCTG‑CAAc > 2:2090010/1‑239 (MQ=255) gTTGCGCTTCTTGCAGGGCATAAGCCTCTGTTTCATTGGCGCTGTGGGATACGCCGCAATTCAGGAATCCTTCGAAGAGGCGGTTTGTATCAAGATCACCGCGCTGATGGCGACCGTGGCGCTGATTGCTCCGCTACTTGGTCCGCTGGTGGGGGCGGCGCGGAGCCATGTGCTGCCCTGGGAGGGGGTGATTGTT‑TTGTTTGACGCAT‑TGGCAGCGAGTTCCTTTTTCGGTGTG‑Cacc > 2:5089/1‑237 (MQ=255) gcTTCTTGCAGGGCATAAGCCTCTGTTTCATTGGCGCTGTGGGATACGCCGCAATTCAGGAATCCTTCGAAGAGGCGGTTTGTATCAAGATCACCGCGCTGATGGCGAACGTGGCGCTGATTGCTCCGCTACTTGGTCCGCTGGTGGGCGCGGCGTGGATCCATGTGCTGCCCTGGGAGGGGATGTTTGTT‑TTGTTTGCCGCAT‑TGGCAGCGATCTCCTTTTTCGGTCTG‑CAACGAGcc < 1:1619252/239‑1 (MQ=255) cttGCAGGGCATAAGCCTCTGTTTCATTGGCGCTGTGGGATACGCCGCAATTCAGGAATCCTTCGAAGAGGCGGTTTGTTTTCAGATCACCGCGCTGATGGCGAAAGTGGCGCCGATTGCTCCGCTACTTGGTCCGCCGGTGGGGGCGGCGTGGATCCATGTGCTGCCCTTGGGGGGGATGTTTGTT‑TTTTTTGCCGCAT‑TGGCTGCGATCTCCTTTTTCGGTCTG‑CAACCAgacctc > 2:1377976/1‑233 (MQ=255) tGCAGGGCATAAGCCTCTGTTTCATTGGCGCTGTGGGATACGCCGCAATTCAGGAATCCTTCGAAGAGGCGGTTTGTATCTAGTTCACCGCGCTGATGGCGAAAGTGGCGCTGATTTCTTCCCTAATTGGGCCCATGGTGGGCGCGGGCTGGATCCAAGTGGTGGCCTCGGGGGGGTAGTTTGTT‑TTGTTTGCCGCAT‑AGGCAACGCGTTCCCTTTTTGGTCTT‑Caaagtaccaag > 2:1731521/1‑227 (MQ=255) aTAAGCCTCTGTTTCATTGGCGCTGTGGGATACGCCGCAATTCAGGAATCCTTCGAAGAGGCGGTTTGTATCAAGATCACCGCGCTGATGGCGAACGTGGCGCTGATTGCTCCGCTACTTGGTCCGCTGGTGGGCGCGGCGTGGATCCATGTGCTGCCCTGGGa > 2:750023/1‑164 (MQ=255) aTAAGCCTCTGTTTCATTGGCGCTGTGGGATACGCCGCAATTCAGGAATCCTTCGAAGAGGCGGTTTGTATCAAGATCACCGCGCTGATGGCGAACGTGGCGCTGATTGCTCCGCTACTTGGTCCGCTGGTGGGCGCGGCGTGGATCCATGTGCTGCCCTGGGa < 1:750023/164‑1 (MQ=255) tGTTTCATTGGCGCTGTGGGATACGCCGCAATTCAGGAATCCTTCGAAGAGGCGGTTTTTATTAAGATCACCGCGCTGATGGCGAACGTGGCGCTGATTGCTCCGCTACTTGGTCCGCTGGTGGGCGCGGCGTGGATCCATGTGCTGCCCTGGGAGGGGATGTTTGTT‑TTGTTTTCCGCAT‑TGGCAGCGAGCTCCTTTTTCGGTCTG‑CAACGAGCCATGCCTGAAACCGcccaccgtat > 2:1725395/1‑231 (MQ=255) tGTTTCATTGGCGCTGTGGGATACGCCGCAATTCAGGAATCCTTCGAAGAGGCGGTTTGTTTCAAGATCCCCGCGCCGATGGCGAACGGGGCGCCGATTGCTCCGCTACATGGGCCGCTTGTGGGGGCGGCGTGGGACCATGTGTTGCCCTGGGGGGGGATGTTTGTT‑TTGTTTGCCGCTT‑TGGCAGGGATCTCCTTTTTTGGCTTG‑CAACTAACCCTGCCTGAAACCGccccccgctc > 2:354112/1‑231 (MQ=255) tGTTTCATTGGCGCTGTGGGATACGCCGCAATTCAGGAATCCTTCGAAGAGGCGGTTTGTATCAAGATCACCGCGCTGATGGCGAACGTGGCGCTGTTTGCTCCGCTACTTGGTCCGCTGGTGGGCGCGGCGTGGGTCCATGGGCTGCCCTGGGGGGGGATGGTTGTT‑TTGTTTGCCGCCT‑TGGCAGCGATCTCCTTTTTCGGTCTG‑CAACTAGCCATTCCTTGAACCGcccaccgtat > 2:1495873/1‑231 (MQ=255) cATTGGCGCTGTGGGATACGCCGCAATTCAGGAATCCTTCGAAGAGGCGGTTTTTATCAAAATCACCGCGCTGATGGCGAACGCGGCGCCGATTGCTCCGCTTCTTGGGCCGCTTGTGGGCGCGGCCTGGATCCATTTGCTGCCCTTGGGGGGGATGTTTTGT‑TTTTTTTCCCCAT‑TGGCCGGCACCTCCCTTTTCGGGCTGCCACCGGCCCAGTCCTGAAACC‑CCCCGCGtattaggc > 2:2081821/1‑234 (MQ=255) ttgtgGTATCCGCTGCAATCAAGGATTCCTCGGAAGAGGCGTTTTGTATAAAGATCACGGCGCGGAGGGCGAGCGTGGCGCTGTTTCCTCCGCTTTTTGGTCCGCTGGGGGGCGCGGCGTGGATCCATGTGCTGCCCTGGGAGGGGATGTTTGTT‑TGTTTTGCCGCAT‑TGGCAGCGATCTCCTTTTTCGGTCGG‑CAACGGGCCATGCCTGAAACCGCCACGCGTATAGGCGAGAAACt < 2:1356989/237‑1 (MQ=255) gccgcAATTCAGGAATCCTTCGAAGAGGCGGTTTGTATCAAGAACACCGCCCTGGAGGCGAAACCGGCGCTGATTGCCCCGCCACTTTGTCCCCTGGGGGGCGCGGCGGGGAGCCATTGCCTGCCCTGGGGGGGGGTGCTTTTT‑TTTGTTGCCGCCT‑TGGCCGCCGACTCCTTTTTTGGGCTG‑CCACGAGCCAACCCGGAAAACCCCCCGCGTACATGCGGGCAACAGtacacggaaga > 2:1919067/1‑229 (MQ=255) cgcAATTCAGGAATCCTTCGAAGAGGCGGTTTTTATCAAGACCCCCGCGCCGGTGGCGAACGGGGCGCCTGTTGCTCCGCTACTTGGTCCGCCGGGGGGCGCGGGGGGGATCCATGTGCTGCCCTGGGGGGGGGGGTTTGTT‑TTGTTTTCCGCAT‑TGGTAGCGGTCTCCTTTTTTGGGCTG‑CACCCAGCCATTCCTGAAACCGCCCCCCGTATAGTCGGGGAACAGttctctaaaaaa > 2:874830/1‑227 (MQ=255) cgcAATTCAGGAATCCTTCGAAGAGGCGGTTTGTATCAAGATCACCGCGCTGATGGCGAACGTGGCGCTGATTGCTCCGCTACTTGGTCCGCTGGTGGGCGCGGCGTGGATCCATGTGCTGCCCTGGGAGGGGATGTTTGTT‑TTGTTTGCCGCAT‑TGGCAGCGATCTCCTTTTTCGGTCTG‑CAACGAGCCATGCCTGAAACCGCCACGCGTATAGGCGAGAAACTGTCACTGaaaaa > 1:1609011/1‑235 (MQ=255) cgcAATTCAGGAATCCTTCGAAGAGGCGGTTTGTATCAAGATCACCGCGCTGATGGCGAACGGGGCGCTGATTGCCCCGCTACTTGGTCCGCTGGTGGGCGCGGCGTGGATCCATGTGCTGCCCTGGGAGGGGATGTTTGTT‑TTGGTTGCCGCAT‑TGGGAGCGATCTCCTTTTTCGGTCTG‑CAACGAGCCATGCCTGAAACCGCCACGCGTATAGGCGAGaa > 1:379305/1‑222 (MQ=255) cgcAATTCAGGAATCCTTCGAAGAGGCGGTTTGTATCAAGATCACCCCGCTGATGGCGAACGTGGCGCTGATTGCTCCGCTACTTGGTCCGCTGGTGGGCGCGGCGTGGATCCATGTGCTGCCCTGGGGGGGGGTGTTTGTT‑TTGTTTGCCGCCT‑TGGCAGCGATCTCCTTTTTCGGTCTG‑CAACGAGCCATGCCCGAAACCGCCCCCCGTATAGGCGAGAAAATGTCACTGAAAGaaa > 2:2237855/1‑238 (MQ=255) cgcAATTCAGGAATCCTTCGAAGAGGCGGTTTGTATCAAAATCACCGCGCCGATGGCGAACGTGGCGCTGATTGCTCCGCGACTTGGGCCGCCGGGGGGCGCCGCGCGGGCTCCTGTGCTGCCCTGGGGGGGGGTGGTTGTT‑TTGTTTGCCCCAA‑TTGGAGCCATCTTCTTTTTCGGTCTG‑TCACGAGCCATGCCTGTAACCGCCCCGCGCATAGGCGCGAAACAGTcgacgtaaga > 2:121284/1‑228 (MQ=255) ccaatttCAGGAACCCTTGGAAGGGGCGGTTTGTATCAAGATCACCGCGCGGTTGGGGAACGGGGCGCTGATTGCCCCGCTACTTGGTCCGCTGGTGGGCGCGGCGTGGATCCATGTGCTGCCCTGGGGGGGGATGTTTGTT‑TTGTTTGCCGCAT‑TGGCAGCGATCTCCTTTTTCGGTCTG‑CAACGAGCCATGCCTGAAACCGCCACGCGTATAGGCGAGAAACTGTCa < 2:857740/224‑1 (MQ=255) cAATTCAGGAATCCTTCGAAGAGGCGGTTTGTATCAAGATCACCGCGCTGATGGCGAAAGTGGCGCTGATTGCTCCGCTACTTGGTCCGCTGGTGGGGGCGGCGTGGATCCATGTGCTGCCCTGGGAGGGGGTGTTTGTT‑TTGTTTGCCGCAT‑TGGCAGCGATCTCCTTTTTTGGGCTG‑CAACGGGCCATGCCTGGAACGGCCACCCGCATAGGCGGGAAACAGTTCCCGAAAAAAccc > 2:922987/1‑237 (MQ=255) gAATCCTTCGAAGAGGCGGTTTGTATCAAGATCACCGCGCTGATGGCGAACGTGGCGCTGATTGCTCCGCTACTTGGTCCGCTGGTGGGCGCGGCGTGGATCCATGTGCTGCCCTGGGAGGGGATGTTTGTT‑TTGTTTGCCGCAT‑TGGCAGCGGTCTCCTTTTTCGGTCTG‑CAACGAGCCATGCCTGAAACCGCCACGCGTATAGGCGAGAAACTTTCACTGAAAGAAc > 1:857740/1‑229 (MQ=255) aacccTGCGGAGAGGCGGTTTGTATCAATATCCCGGCGCTGAGGGCGAACGTGGCGCTGATGGTGCGGCTACTTGGTCCGCTGGTGGACGCGGCGTGGATCCAGGTGCTGCCCTGGGGGGGGATTTTTGTT‑TTGTTTGCCGCAA‑TGGCAGCGTTCTCTTTTTTCGGTTTG‑CAACGACCAAGGCCGGAAACCGCCACGCGTATAGGCGAGAATCTGTCACTGAAAGAACTCGGTCGt < 2:1558270/233‑1 (MQ=255) aacccTGCGAAGAGGCGGTTTGTTTCAAGACCCCCGCGCTGAGGGCGACCGGGGCGTGGATTGCTCGGCTACTGGGTCCGCTGGTGGGCGGGGCGTGGATCCATGTGCTGCCCGGGGAGGGGATGTTTTTT‑TTGTTTGCCGCAT‑TGGCAGCGATCTCCTTTTTCGGTCTG‑CAACGAGCCATGCCTGAAACCGCCACGCGTATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGt < 2:1984240/233‑1 (MQ=255) aaTCTTGCGAGGAGGCGGTTGGTATCAAGATCCCCGGGCGGTGGGGGAACGGGGCGCTGATTGCCCCGCTACTTGGCCGGCTGGTGGGCGGGGCGTGGATCCATGTGCTGCCCTGGGGGGGGATTTTTGTT‑TTGTTTGCCGCAT‑TGGCAGCGATCTCCTTTTTCGGTCTG‑CAACGAGCCATGCCTGAAACCGCCACGCGTATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCg < 2:1003909/235‑1 (MQ=255) aaTCCTTCAAAGAGGCGGTTTTGATCAAGACACCCGCGCTGATGGCGGCGGGGGCACTGATTGCGCCGCTACTGGGTCCGGTGGTGGGCGCGGCGTGGATCCATTGGCTCCCCTGGGAGGGAAT‑TTTGTTGTTGTGTGCGGCTT‑GGGCAGCGATCTCCTTTTTCGGTCGG‑CAACGAGCCGTGCCTGAAACCGCCACGCGTATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGt < 2:1614255/236‑1 (MQ=255) cTTCGAAGAGGCGGTTTGTATCAAGATCCCCGCGCTGATGGCGAACGTGGCGCTGATTGCTCCGCTACTTGGTCCGCTGGTGGGCGCGGCGTGGATCCATGTGCTGCCCTGGGAGGGGATGTTTGTT‑TTGTTTGCCGCAT‑TGGCAGCGATCTCCTTTTTCGGTCTG‑CAACGAGCCATGCCTGAAACCGCCACGCGTATAGGCGAGAAACt < 2:1482424/210‑1 (MQ=255) cTTCGAAGAGGCGGTTTGTATCAAGATCACCGCGCTGATGGCGAACGTGGCGCTGATTGCTCCGCTACTTGGTCCGCTGGTGGGCGCGGCGTGGATCCATGTGCTGCCCTGGGGGGGGATGTTTGTT‑TTGTTTGCCGCAT‑TGGCAGCGATCTCCTTTTTCGGTCTG‑CAACGAGCCATGCCTGAAACCGCCACGCGTATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACtata > 1:1984240/1‑239 (MQ=255) cTTCGAAGAGGCGGTTTGTATCAAGATCACCGCGCTGATGGCGAACGTGGCGCTGATTGCTCCGCTACTTGGTCCGCTGGTGGGCGCGGCGTGGATCCATGTGCTGCCCTGGGAGGGGATGTTTGTT‑TTGTTTGCCGCAT‑TGGCAGCGATCTCCTTTTTCGGTCTG‑CAACGAGCCATGCCTGAAACCGCCACGCGTATAGGCGAGAAACt > 1:1482424/1‑210 (MQ=255) cGAAGAGGCTGTTTGTATCAAGATCACCGCGCTGATGGCGAACGTGGCGCTGATTGCTCCGCTACTTGGTCCGCTGGTGGGCGCGGCGTGGATCCATGTGCTGCCCTGGGAGGGGATGTTTGTT‑TTGTTTGCCGCAT‑TGGCAGCGATCTCCTTTTTCGGTCTG‑CAACGAGCCATGCCTGAAACCGCCACGCGTATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGc < 1:1960769/239‑1 (MQ=255) cGAAGAGGCGGTTTGTATCAAGATCACCGCGCTGATGGCGAACGTGGCGCTGATTGCTCCGCTACTTGGTCCGCTGGTGGGCGCGGCGTGGGTCCATGTGCTGCCCTGGGGGGGGATGTTTGTT‑TTGTTTGCCGCAT‑TGGCAGCGATCTCCTTTTTCGGTCTG‑CAACGAGCCATGCCTGAAACCGCCACGCGTATAGGCGAGAAACTGTCACTGAAAGAACTCGGGCGTGACTATAAGc > 1:1670223/1‑239 (MQ=255) aaGAGGCGGTTTTTATCAAGATCACCGCGCTGATGGCGAACGTGGCGCTGATTGCTCCGCTACTTGGTCCGCTGGTGGGCGCGGCGTGGATCCATGTGCTGCCCTGGGGGGGGATGTTTGTT‑TTGTTTGCCGCAT‑TGGCAGCGATCTCCTTTTTCGGTCTG‑CAACGAGCCATGCCTGAAACCGCCACGCGTATAGGCGAGAAAATGTCACTGAAAGAAATCGGTCGGGACTATaa > 1:476430/1‑235 (MQ=255) gCGGTTTTTATCAAGATCACCGCGCTGATGGCGGACGTTGCGCTGATTGCTCCGCTACTTGGTACGCTGGGGGGCGCGGCGTGGATCCATGTTCTGCCCTGGGGGGGGGTGTTTGTG‑TTGTTTGCCGCGT‑TGGCAGCGATATCCTTTTTTGGTCTG‑CAACGCGCCATGCCTGAAACCGCCACGCGTATATGCGGGAATCTGTGCCTGAAAGAATCCGGGGGTGCCTATAAGCTggggtc > 2:949042/1‑235 (MQ=255) gCGGTTTGTATCAAGATCACCGCGCTGATGGCGAACGTGGCGCTGATTGCTCCGCTACTTGGTCCGCTGGTGGGCGCGGCGTGGATCCATGTGCTGCCCTGGGAGGGGATGTTTGTT‑TTGTTTGCCGCAT‑TGGCAGCGATCTCCTTTTTCGGTCTG‑CAACGAGCCATGCCTGAAACCGCCACGCGTATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGGGACTATAAGCTGGt > 1:126200/1‑236 (MQ=255) ggTTTGTATCAAGATCACCGCGCTGATGGCGACCGTGGCGCTGATTGCTCCGCTACTTGGTCCGCTGGTGGGCGCGGCGTGGGTCCCTGTGCTGCCCTGGGGGGGGGTGGTTGTT‑TTGTTTGCCGCCT‑TGGCAGCGATCTCCTTTTTCGGTCTG‑CAACGAGCCCTGCCCGAAAACGCCACGCGTATAGGCGAGAAAATGTCCCTGAAAGAACTCGGGTGTGAATACAAACTgggggtgg > 2:493487/1‑233 (MQ=255) aTCAAGATCCCCGCGCTGAGGGCGAACGTGGCGCTGATTGCTCCGCTACTTGGTCCGCTGGTGGGCGCGGCGTGGATCCATGTGCTGCCCTGGGAGGGGATGTTTGTT‑TTGTTTGCCGCAT‑TGGCAGCGATCTCCTTTTTCGGTCTG‑CAACGAGCCATGCCTGAAACCGCCACGCGTATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACgg < 1:2237855/239‑1 (MQ=255) ccccGCGCTGAGGGCACCCGGGGCGCTGTTGGCTCCGCTCCTGGGTCCGCTGGTGGGCGCGGCGTGGATCCTTGTGTTGCCCGGGGGGGGGTGTTTTGTT‑TTGTTTGCCGCTT‑GGGCGGCGATCTCCTTTTTCGGTCGG‑CAACGAGCCATGCCTGAAACCGCCACGCGTATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCttt < 2:1419376/236‑1 (MQ=255) aCCGCGCTGATGGCGAACGTGGCGCTGATTGCTCCGCTACTTGGTCCGCTGGTGGGCGCGGCGTGGATCCATGTGCTGCCCTGGGAGGGGATGTTTGTT‑TTGTTTGCCGCAT‑TGGCAGCGATCTCCTTTTTCGGTCTG‑Caa > 2:1400108/1‑141 (MQ=255) aCCGCGCTGATGGCGAACGTGGCGCTGATTGCTCCGCTACTTGGTCCGCTGGTGGGCGCGGCGTGGATCCATGTGCTGCCCTGGGAGGGGATGTTTGTT‑TTGTTTGCCGCAT‑TGGCAGCGATCTCCTTTTTCGGTCTG‑Caa < 1:1400108/141‑1 (MQ=255) aCCGCGCTGATGGCGAACGTGGCGCTGATTGCTCCGCTACTTGGTCCGCTGGTGGGCGCGGCATGGATCCATTAGCTGCCCTGGGAGGGGATGTTTGTT‑TTGTTTGCCGCAT‑TGGCGGCGATCTCCTTTTTCGGTCTG‑CAACGAGCCATGCCTGAAACCGCCACGCGGATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTtgt > 2:1310138/1‑239 (MQ=255) gggacggTGGCGCTGTTTGCTCCGCTCCTGGGTCGGCTGGTGGGCGCGGGGTGGATCCTTGTGCTGCCCTGGGGGGGGATGTTTGTT‑TTGTTTGCCGCAT‑TGGCAGCGATCTCCTTTTTCGGTCTG‑CAACGAGCCATGCCTGAAACCGCCACGCGTATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGt < 2:534926/186‑1 (MQ=255) tGGCGCTGATTGCTCCGCTACTTGGTCCGCTGGTGGGCGCGGCGTGGTTCCATGTGCTGCCCTGGGAGGGGTTGTTTGTT‑TTGTTTGCCGCAT‑TGGCAGCGATCTCCTTTTTCGGTCTG‑CAACGAGCCATGCCTGAAACCGCCATGCGTCTAGGCGAGaa > 1:727936/1‑160 (MQ=255) cgcTGATTGCTCCGCTACTTGGTCCGCTGGTGGGCGCGGCGTGGATCCATGTGCTGCCCTGGGAGGGGATGTTTGTT‑TTGTTTGCCGCAT‑TGGCAGCGATCTCCTTTTTCGGTCTG‑CAACGAGCCATGCCTGAAACCGCCACGCGTATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGc > 1:534926/1‑192 (MQ=255) tttGCCCCGCTACTGGGTCCGCTGGTGGGCGCGGCGTGGATCCAGGTGCTGCCCTGGGAGGGGATGTTTGTT‑TTGTTTGCCGCAT‑TGGCAGCGATCTCCTTTTTCGGTCTG‑CAACGAGCCATGCCTGAAACCGCCACGCGTATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGgcgctggcgctg < 1:502838/230‑1 (MQ=255) tACTTGGTCCGCTGGTGGGCGCGGCGTGGATCCATGTGCTGCCCTGGGAGGGGATGTTTGTT‑TTGTTTGCCGCAT‑TGGCAGCGATCTCCTTTTTCGGTCTG‑CAACGAGCCATGCCTGAAACCGCCACGCGTATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGGGCTGGGGCGGGGGTTGGtgt > 2:502838/1‑229 (MQ=255) ggTCCGCTGGTGGGCGCGGCGTGGATCCATGTGCTGCCCTGGGAGGGGATGTTTGTT‑TTGTTTGCCGCAT‑TGGCAGCGATCTCCTTTTTCGGTCTG‑CAACGAGCCATGCCTGAAACCGCCACGCGTATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTg < 1:46205/174‑1 (MQ=255) ggTCCGCTGGTGGGCGCGGCGTGGATCCATGTGCTGCCCTGGGAGGGGATGTTTGTT‑TTGTTTGCCGCAT‑TGGCAGCGATCTCCTTTTTCGGTCTG‑CAACGAGCCATGCCTGAAACCGCCACGCGTATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTg > 2:46205/1‑174 (MQ=255) tCCGCTGGTGGGCGCGGCGTGGATCCATGTGCTGCCCTGGGAGGGGATTTTTGTT‑TTGTTTGCCGCAT‑TGGCAGCGATCTCCTTTTTCGGTCTG‑CAACGAGCCATGCCTGAAACCGCCACGCGTATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCTGGCGCTGGGATTCGTTAGTCTGCCGTTGCTg < 1:1698786/238‑1 (MQ=255) ggtggtggGCGGGGGGGGGATCCAGGTGCGGCCGTGGGAGGGGATGTTTGTT‑TTGTGTGCCGCTT‑TGGCAGCGATCTCCTTTTTCGGTCGG‑CAACGAGCCATGCCTGAAACCGCCACGCGTATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCTGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGt < 2:227940/237‑1 (MQ=255) cTGGTGGGGGGGGTGGGGTTCCAGGTGCTGCCCGGGGAGGGGGTGTTTGTT‑TTGTTGGCCGCAT‑TGGCAGGGATCTCCTTTTTCGGTCTG‑CAACGAGCCATGCCTGAAACCGcc < 2:1441711/114‑1 (MQ=255) tggtggGCGCGGCGTGGATCCATGTGCTGCCCTGGGAGGGGATGTTTGTT‑TTGTTTGCCGCAT‑TGGCAGCGATCTCCTTTTTCGGTCTG‑CAACGAGCCATGCCTGAAACCGCCACGCGTATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACtat < 1:763980/161‑1 (MQ=255) tggtggGCGCGGCGTGGATCCATGTGCTGCCCTGGGAGGGGATGTTTGTT‑TTGTTTGCCGCAT‑TGGCAGCGATCTCCTTTTTCGGTCTG‑CAACGAGCCATGCCTGAAACCGCCACGCGTATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACtat > 2:763980/1‑161 (MQ=255) gcggtgTGGATCCGTGTGCTGCCCTGGGAGTGTATGTTTGTT‑TTGTTTGCCGCAT‑TGGCGGCGTCCTCCTTTTCCGTTCTG‑AAGCGAGCCATGCCTGAAACCGCCACGCGTATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCTGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGGATCGCCCa < 1:1087952/239‑1 (MQ=255) cggcgTGGATCCATGTGCTGCCCTGGGAGGGGATGTTTGTT‑TTGTTTGCCGCAT‑TGGCAGCGATCTCCTTTTTCGGTCTG‑CAACGAGCCATGCCTGAAACCGCCACGCGTATAg > 1:1441711/1‑114 (MQ=255) cggggATCATTGTGCTGCCTGGGGAGGGTAGTTTTGTT‑TTTTTGGCCGCAT‑GGGCAGCGACCTCCTTTTTCGGTCTG‑CAACGACCCATGCCTGAAACCGCCACGCGTATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGggcgc < 2:1139708/186‑1 (MQ=255) ggATCCTTGTGCTGCCCTGGGGGGGGATGTTTGTT‑TTGTTGGCCGATT‑GGGCAGGGATCTCTTTTTCCGGTCTG‑CAACGAGCCATGCCTGAAACCGCCACGCGTATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCTGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGGATCGCCCAGTCGcc < 1:2210810/238‑1 (MQ=255) tgcCCTGGGAGGGGATGTTTGTT‑TTGTTTGCCGCAT‑TGGCAGCGATCTCCTTTTTCGGTCTG‑CAACGAGCCATGCCTGAAACCGcc < 2:1075732/86‑1 (MQ=255) tgcCCTGGGAGGGGATGTTTGTT‑TTGTTTGCCGCAT‑TGGCAGCGATCTCCTTTTTCGGTCTG‑CAACGAGCCATGCCTGAAACCGcc > 1:1075732/1‑86 (MQ=255) cccTGGGAGGGGATGTTTGTT‑TTGTTTGCCGCAT‑TGGCAGCGATCTCCTTTTTCg > 1:1468559/1‑55 (MQ=255) cccTGGGAGGGGATGTTTGTT‑TTGTTTGCCGCAT‑TGGCAGCGATCTCCTTTTTCg < 2:1468559/55‑1 (MQ=255) cccTGGGA‑GGGATGTTTGTT‑TTGTTTGCCGCAT‑TGGCAGCGATCTCCTTTTTCGGTCTG‑CAACGAGCCATGCCTGAAACCGCCACGCGTATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCTGGCGCTGGGATTCGTTAGTCTGCCGTTg < 1:1523464/200‑1 (MQ=255) cTGGGAGGGGATGTTTTTT‑TTGTTGGCCCCAT‑TGGCAGCGATCTCCTTTTTCGGGCTG‑CAACGAGCCATGCCTAAAACCGcc < 2:2086851/82‑1 (MQ=255) ggAGGGGATTTTTGTT‑TTGTTTCCCGCAT‑TGGCAGCGATCTCCTTTTTCGGTCTG‑CAACGAGCCATGCCTGAAACCGCCACGCGTATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCTGGCGCTGGGATTCGTTAGTCTGCCGt < 1:556670/194‑1 (MQ=255) | TTATCGTCACCTGTCTGGCAATATTGCTGGCGCAAAACATTGAACAATTCACCCTGTTGCGCTTCTTGCAGGGCATAAGCCTCTGTTTCATTGGCGCTGTGGGATACGCCGCAATTCAGGAATCCTTCGAAGAGGCGGTTTGTATCAAGATCACCGCGCTGATGGCGAACGTGGCGCTGATTGCTCCGCTACTTGGTCCGCTGGTGGGCGCGGCGTGGATCCATGTGCTGCCCTGGGAGGGGATGTTTGTT‑TTGTTTGCCGCAT‑TGGCAGCGATCTCCTTTTTCGGTCTG‑CAACGAGCCATGCCTGAAACCGCCACGCGTATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCTGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGGATCGCCCAGTCGCC > NC_000913/883947‑884400 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |