Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,296,764	0	T	G	34.3%	151.1 / 16.1	67	L87F (TTA→TTC)	ccmB	heme export ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base T (9/35);  new base G (23/0);  total (32/35)
Fisher's exact test for biased strand distribution p-value = 5.29e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

ACACCAGTATGCTGAGCAGCACACCACCGCGCTTAAGTCCCACTGTCAGCGCCACGCCCGGTGCACCGAGAAAGCCAAGCGTAGGCGTTCCCAGCAGCAGCGTCAGCGCCATCACTTGCCAGCCATAAACATCCATTCCCAGTAGCATTGCTACCAGTGGCGAAAGGATGAGTAACGGCAGACCGGTTACCATCCAGTGCGCCATCACCTTCGCCAGCACAACGGCGGGCAAGGGTAACGGCAACAACATCAA‑TTGTTCAAGACTGCCGTCCTGCAAATCGTCACGGAACAGTCGTTCCAGCGCCAGCAAGGATGAAAGCAGCGCAGCAACCCAGATAATGCCCGGTGCAATACGCGCCAGCAGTTGCGGCTCCGGA‑CCGATACTGAGCGGAAAAAGGGTAATTACAATCAGGAAGAACCACAGCGGGTTGGCGATTTCGGCGCTATGGCGAAACGCTACACGCA  >  NC_000913/2296529‑2296993                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                        acacCAGTATGCTGAGCAGCACACCACCGCGCTTAAGTCCCACTGTCAGCGCCACGCCCGGTGCACCGAGAAAGCCAAGCGTAGGCGTTCCCAGCAGCAGCGTCAGCGCCATCACTTGCCAGCCATAAACATCCATTCCCAGTAGCATTGCTACCAGTGGCGAAAGGATGAGTAACGGCAGACCGGTTACCATCCAGTGCGCCATCACCTTCGCCAGCACAACGGCGGGCAAGGGTAAc                                                                                                                                                                                                                                      <  1:1592463/239‑1 (MQ=255)         aTGCTGAGCAGCACACCACCGCGCTTAAGTCCCACTGTCAGCGCCACGCCCGGTGCACCGAGAAAGCCAAGCGTAGGCGTTCCCAGCAGCAGCGTCAGCGCCATCACTTGCCAGCCATAAACATCCATTCCCAGTAGCATTGCTACCAGTGGCGAAAGGATGAGTAACGGCAGACCGGTTACCATCCAGTGCGCCATCACCTTCGCCAGCACAACGGCGGGCAAGGGTAACGGca                                                                                                                                                                                                                                  <  1:166160/235‑1 (MQ=255)               agcatcACACCACCGCGCTTAAGTCCCACTGTCAGCGCCACGCCCGGTGCACCGAGAAAGCCAAGCGTAGGCGTTCCCAGCAGCAGCGTCAGCGCCATCACTTGCCAGCCATAAACATCCATTCCCAGTAGCATTGCTACCAGTGGCGAAAGGATGAGTAACGGCAGACCGGTTACCATCCAGTGCGCCATCACCTTCGCCAGCACAACGGCGGGCAAGGGGAACGGccacaaacacaa                                                                                                                                                                                                                        >  1:1197339/1‑233 (MQ=255)               agcagcACACCACCGCGCTTAAGTCCCACTGTCAGCGCCACGCCCGGTGCACCGAGAAAGCCAAGCGTAGGCGTTCCCAGCAGCAGCGTCAGCGCCATCACTTGCCAGCCATAAACATCCATTCCCAGTAGCATTGCTACCAGTGGCGAAAGGATGAGTAACGGCAGACCGGTTACCATCCAGTGCGCCATCACCTTCGCCAGCACAACGGCGGGTCAGGGTAACGGCAACAACATaaa                                                                                                                                                                                                                        >  2:1127635/1‑239 (MQ=255)               agcagcACACCACCGCGCTTAAGTCCCACTGTCAGCGCCACGCCCGGTGCACCGAGAAAGCCAAGCGTAGGCGTTCCCAGCAGCAGCGTCAGCGCCATCACTTGCCAGCCATAAACATCCATTCCCAGTAGCATTGCTACCAGTGGCGAAAGGATGAGTAACGGCAGACCGGTTACCATCCAGTGCGCCATCACCTTCGCCAGCACAACGGCGGGCAAGGGGAACGGCCACAACATCaa                                                                                                                                                                                                                        >  1:1553831/1‑239 (MQ=255)                gcagcaCACCCCCGCGATTAAGTCCCCCTGCCAGCGCCACCCCCGGTGCCCCGAGAAAGCCAAGCGTAGGCGTTCCCAGCAGCAGCGTCAGCGCCATCACTTGCCAGCCATAAACATCCATTCCCAGTAGCATTGCTACCAGTGGCGAAAGGATGAGTAACGGCAGACCGGTTACCATCCAGTGCGCCATCACCTTCGCCAGCACAACGGCGGGCAAGGGTAACGGCAACAACATCaa                                                                                                                                                                                                                        <  2:1553831/238‑1 (MQ=255)                           ccGCGCTCAAGTCCCACTGTCAGCGCCACGCCCGGTGCACCGAGAAAGCCAAGCGTAGGCGTTCCCAGCAGCAGCGTCAGCGCCATCACTTGCCAGCCATAAACATCCATTCCCAGTAGCATTGCTACCAGTGGCGAAAGGATGAGTAACGGCAGACCGGTTACCATCCAGTGCGCCATCACCTTCGCCAGCACAACGGCGGGCCAAGGGAACGGCAACAAAATCAATTTGTTCag                                                                                                                                                                                                               >  1:310362/1‑235 (MQ=255)                              cgcTTAAGTCCCACTGTCAGCGCCACGCCCGGTGCACCGAGAAAGCCAAGCGTAGGCGTTCCCAGCAGCAGCGTCAGCGCCATCACTTGCCAGCCATAAACATCCATTCCCAGTAGCATTGCTACCAGTGGCGAAAGGATGAGTAACGGCAGACCGGTTACCATCCAGTGCGCCATCACCTTCGCCAGCACAACGGCGGGCCACGGGAACTGCACAAACCCCAA‑ATGTTCAAAAcac                                                                                                                                                                                                          >  2:1184003/1‑235 (MQ=255)                              cgcTTAAGTCCCACTGTCAGCGCCACGCCCGGTGCACCGAGAAAGCCAAGCGTAGGCGTTCCCAGCAGCAGCGTCAGCGCCATCACTTGCCAGCCATAAACATCCATTCCCAGTAGCATTGCTACCAGTGGCGAAAGGATGAGTAACGGCAGACCGGTTACCATCCAGTGCGCCATCACCTTCGCCAGCACAACGGCGGGCAAGGGGAACGGCAACAACCACAA‑ATGTTCAAAACTAcc                                                                                                                                                                                                        >  2:94724/1‑239 (MQ=255)                                   aaGTCCCACTGTCAGCGCCACGCCCGGTGCACCGAGAAAGCCAAGCGTAGGCGTTCCCAGCAGCAGCGTCAGCGCCATCACTTGCCAGCCATAAACATCCATTCCCAGTAGCATTGCTACCAGTGGCGAAAGGATGAGTAACGGCAGACCGGTTACCATCCAGTGCGCCATCACCTTCGCCAGCACAACGGCGGGCAAGGGTAACGGCAACAACATCaa                                                                                                                                                                                                                        >  2:433082/1‑219 (MQ=255)                                   aaGTCCCACTGTCAGCGCCACGCCCGGTGCACCGAGAAAGCCAAGCGTAGGCGTTCCCAGCAGCAGCGTCAGCGCCATCACTTGCCAGCCATAAACATCCATTCCCAGTAGCATTGCTACCAGTGGCGAAAGGATGAGTAACGGCAGACCGGTTACCATCCAGTGCGCCATCACCTTCGCCAGCACAACGGCGGGCAAGGGTAACGGCAACAACATCaa                                                                                                                                                                                                                        <  1:433082/219‑1 (MQ=255)                                          aCTGTCAGCGCCACGCCCGGTGCACCGAGAAAGCCAAGCGTAGGCGTTCCCAGCAGCAGCGTCAGCGCCATCACTTGCCAGCCATAAACATCCATTCCCAGTAGCATTGCTACCAGTGGCGAAAGGATGAGTAACGGCAGACCGGTTACCATCCAGTGCGCCATCACCTTCGCCAGCACAACGGCGGGCAAGGGt                                                                                                                                                                                                                                         >  1:742895/1‑195 (MQ=255)                                          aCTGTCAGCGCCACGCCCGGTGCACCGAGAAAGCCAAGCGTAGGCGTTCCCAGCAGCAGCGTCAGCGCCATCACTTGCCAGCCATAAACATCCATTCCCAGTAGCATTGCTACCAGTGGCGAAAGGATGAGTAACGGCAGACCGGTTACCATCCAGTGCGCCATCACCTTCGCCAGCACAACGGCGGGCAAGGGt                                                                                                                                                                                                                                         <  2:742895/195‑1 (MQ=255)                                                              tGCACCGAGAAAGCCAAGCGTAGGCGTTCCCAGCAGCAGCGTCAGCGCCATCACTTGCCAGCCATAAACATCCATTCCCAGTAGCATTGCTACCAGTGGCGAAAGGATGAGTAACGGCAGACCGGTTACCATCCAGTGCGCCATCACCTTCGCCAGCACAACGGCGGGCAAGGGTAACGGCAACAACATCaa                                                                                                                                                                                                                        <  2:377258/192‑1 (MQ=255)                                                              tGCACCGAGAAAGCCAAGCGTAGGCGTTCCCAGCAGCAGCGTCAGCGCCATCACTTGCCAGCCATAAACATCCATTCCCAGTAGCATTGCTACCAGTGGCGAAAGGATGAGTAACGGCAGACCGGTTACCATCCAGTGCGCCATCACCTTCGCCAGCACAACGGCGGGCAAGGGTAACGGCAACAACATCaa                                                                                                                                                                                                                        >  1:377258/1‑192 (MQ=255)                                                                     agaAAGCCAAGCGTAGGCGTTCCCAGCAGCAGCGTCAGCGCCATCACTTGCCAGCCATAAACATCCATTCCCAGTAGCATTGCTACCAGTGGCGAAAGGATGAGTAACGGCAGACCGGTTACCATCCAGTGCGCCATCACCTTCGCCAGCACAACGGCGGGGCAGGGGAAAGGCAACAACATCAA‑ATGTTCAAGAATGCCGGCCTGGAAATCGTCACGGAACAGACGTTCAAGCGCCAg                                                                                                                                                                 >  2:114605/1‑239 (MQ=255)                                                                                    ggCGTTCCCAGCAGCAGCGTCAGCGCCATCACTTGCCAGCCATAAACATCCATTCCCAGTAGCATTGCTACCAGTGGCGAAAGGATGAGTAACGGCAGACCGGTTACCATCCAGTGCGCCATCACCTTCGCCAGCACAACGGCGGGCAAGGGGAACGGCAACAACAACAA‑ATTGTCAAAACCGCCGCCCCTCAAAATGGCACGGGAAAGACCGTCCAGCGCCCGCAAGGATGgaaagac                                                                                                                                                  >  2:237633/1‑235 (MQ=255)                                                                                          cccAGCAGCAGCGTCAGCGCCATCACTTGCCAGCCATAAACATCCATTCCCAGTAGCATTGCTACCAGTGGCGAAAGGATGAGTAACGGCAGACCGGTTACCATCCAGTGCGCCATCACCTTCGCCAGCACAACGGCGGGCAAGGGGAACGGCAACAACATCAA‑TTGTTCAAGACTGCCGGCCTGCAAAACGTCACGGGACAAGCGGTTCACCGCCACCACGGATGAAAggagcgcaac                                                                                                                                            >  2:174902/1‑237 (MQ=255)                                                                                                agcagcGTCAGCGCCATCACTTGCCAGCCATAAACATCCATTCCCAGTAGCATTGCTACCAGTGGCGAAAGGATGAGTAACGGCAGACCGGTTACCATCCAGTGCGCCATCACCTTCGCCAGCACAACGGCGGGCAAGGGTAACGGCAACAACATCAA‑TTGTTCAAGACTGCCGTCCTGCAAATCGTCACGGAACAGTCGTTCCAGCGCCAGCAAGGATGAAAGCAGCGCAGCAAcc                                                                                                                                        <  1:315978/237‑1 (MQ=255)                                                                                                  cagcGTCAGCGCCATCACTTGCCAGCCATAAACATCCATTCCCAGTAGCATTGCTACCAGTGGCGAAAGGATGAGTAACGGCAGACCGGTTACCATCCAGTGCGCCATCACCTTCGCCAGCACAACGGCGGGCAAGGGTAACGGca                                                                                                                                                                                                                                  <  1:697475/146‑1 (MQ=255)                                                                                                  cagcGTCAGCGCCATCACTTGCCAGCCATAAACATCCATTCCCAGTAGCATTGCTACCAGTGGCGAAAGGATGAGTAACGGCAGACCGGTTACCATCCAGTGCGCCATCACCTTCGCCAGCACAACGGCGGGCAAGGGGAACGGca                                                                                                                                                                                                                                  >  2:697475/1‑146 (MQ=255)                                                                                                            gcCATCACTTGCCAGCCATAAACATCCATTCCCAGTAGCATTGCTACCAGTGGCGAAAGGATGAGTAACGGCAGACCGGTTACCATCCAGTGCGCCATCACCTTCGCCAGCACAACGGCGGGCAAGGGTAACGGCAACAACATCAA‑TTGTTCAAGACTGCCGTCCTGCAAATCGTCACGGAACAGTCGTTCCAGCGCCAGCAAGGATGAAAGCAGCGCAGCAACCCAGATAATGcc                                                                                                                             <  1:738615/236‑1 (MQ=255)                                                                                                               aTCACTTGCCAGCCATAAACATCCATTCCCAGTAGCATTGCTACCAGTGGCGAAAGGATGAGTAACGGCAGACCGGTTACCATCCAGTGCGCCATCACCTTCGCCAGCACAACGGCGGGCAAGGGGGACGGCAACAACATCAA‑TTGTTCAAAACCGCCGGCCCTCAAATCGTCACGGAACAGCCGGTCCAAGGCCGGCAAAGGTGAAAGCAGCGCAGCAGCCCCGAGAAAGccgtgcgc                                                                                                                       >  2:111822/1‑233 (MQ=255)                                                                                                                tCACTTGCCAGCCATAAACATCCATTCCCAGTAGCATTGCTACCAGTGGCGAAAGGATGAGTAACGGCAGACCGGTTACCATCCAGTGCGCCATCACCTTCGCCAGCACAACGGCGGGCAAGGGTAACGGCAACAACATCAA‑TTGTTCAAGACTGCCGTCCTGCAAATCGt                                                                                                                                                                                          <  1:898315/171‑1 (MQ=255)                                                                                                                 cACTTGCCAGCCATAAACATCCATTCCCAGTAGCATTGCTACCAGTGGCGAAAGGATGAGTAACGGCAGACCGGTTACCATCCAGTGCGCCATCACCTTCGCCAGCACAACGGCGGGCAAGGGTAACGGCAACAACATCAA‑TTGTTCAAGACTGCCGTCCTGCAAATCGTCACGGAACAGTCGTTCCAGCGCCAGCAAGGATGAAAGCAGCGCAGCAACCCAGATAATGcc                                                                                                                             <  1:284696/231‑1 (MQ=255)                                                                                                                   cTTGCCAGCCATAAACATCCATTCCCAGTAGCATTGCTACCAGTGGCGAAAGGATGAGTAACGGCAGACCGGTTACCATCCAGTGCGCCATCACCTTCGCCAGCACAACGGCGGGCAAGGGGAACCGCAACAACATCAA‑ATTTTCAAAACTGCCGGCCTGCAAATCCCTACGGAAAAGTCGTTCCAACGCCAACAAAGATGAAAACAGCGCAGCAAACCAGAAAATGCCCCGGGCaaaa                                                                                                                   >  2:1393757/1‑237 (MQ=255)                                                                                                                          gccaTAAACATCCATTCCCAGTAGCATTGCTACCAGTGGCGAAAGGATGAGTAACGGCAGACCGGTTACCATCCAGTGCGCCATCACCTTCGCCAGCACAACGGCGGGCAAGGGGAACGGCAACAACATCAA‑TTGTTCAAGACTGCCGACCCGCAAATCGTCACGGAACAg                                                                                                                                                                                >  2:898315/1‑171 (MQ=255)                                                                                                                           ccaTAAACATCCATTCCCAGTAGCATTGCTACCAGTGGCGAAAGGATGAGTAACGGCAGACCGGTTACCATCCAGTGCGCCATCACCTTCGCCAGCACAACGGCGGGCAAGGGGAACGGCAACAACATCAA‑TTGTTCAAGAATGCCGTCCTGCAAATAGTCCCGGAACAATCGTTCCAGCGCCAGCAAGGGTGAAAGCAACGCAGCAACCCAGATAAAGCCCGGTGGaaaa                                                                                                                   >  2:284696/1‑229 (MQ=255)                                                                                                                            caTAAACATCCATTCCCAGTAGCATTGCTACCAGTGGCGAAAGGATGAGTAACGGCAGACCGGTTACCATCCAGTGCGCCATCACCTTCGCCAGCACAACGGCGGGCAAGGGTAACGGCAACAACATCAA‑TTGTTCAAGACTGCCGTCCTGCAAATCGTCACGGAACAGTCGTTCCAGCGCCAGCAAGGATGAAAGCAGCGCAGCAACCCAGATAATGCCCGGTGCAATAcgcg                                                                                                               <  2:1252034/234‑1 (MQ=255)                                                                                                                                   aTCCATTCCCAGTAGCATTGCTACCAGTGGCGAAAGGATGAGTAACGGCAGACCGGTTACCATCCAGTGCGCCATCCCCTTCGCCAGCACAACGGCGGGCAAGGGTAACGGCAACAACATCAA‑TTGTTCAAGACTGCCGTCCTGCAAATCGTCa                                                                                                                                                                                        <  1:1492386/154‑1 (MQ=255)                                                                                                                                   aTCCATTCCCAGTAGCATTGCTACCAGTGGCGAAAGGATGAGTAACGGCAGACCGGTTACCATCCAGTGCGCCATCACCTTCGCCAGCACAACGGCGGGCAAGGGTAACGGCAACAACATCAA‑TTGTTCAAGACTGCCGTCCTGCAAATCGTCACGGAACAGTCGTTCCAGCGCCAGCAAGGATGAAAGCAGCGCAGCAACCCAGATAATGCCCGGTGCAATACGCGCCAGCAGTTGCg                                                                                                   <  1:912618/239‑1 (MQ=255)                                                                                                                                     ccATTCCCAGTAGCATTGCTACCAGTGGCGAAAGGATGAGTAACGGCAGACCGGTTACCATCCAGTGCGCCATCACCTTCGCCAGCACAACGGCGGGCAAGGGTAACGGcaac                                                                                                                                                                                                                                <  2:125222/113‑1 (MQ=255)                                                                                                                                     ccATTCCCAGTAGCATTGCTACCAGTGGCGAAAGGATGAGTAACGGCAGACCGGTTACCATCCAGTGCGCCATCACCTTCGCCAGCACAACGGCGGGCAAGGGTAACGGcaac                                                                                                                                                                                                                                >  1:125222/1‑113 (MQ=255)                                                                                                                                     ccATTCCCAGTAGCATTGCTACCAGTGGCGAAAGGATGAGTAACGGCAGACCGGTTACCATCCAGTGCGCCATCACCTTCGCCAGCACAACGGCGGGCAAGGGTAACGGCAACAACATCAA‑TTGTTCAAGACTGCCGTCCTGCAAATCGTCACGGAACAGTCGTTCCAGCGCCAGCAAGGATGAAAGCAGCGCAGCAACCCAGATAATGcc                                                                                                                             <  2:91603/211‑1 (MQ=255)                                                                                                                                     ccATTCCCAGTAGCATTGCTACCAGTGGCGAAAGGATGAGTAACGGCAGACCGGTTACCATCCAGTGCGCCATCACCTTCGCCAGCACAACGGCGGGCAAGGGTAACGGCAACAACATCAA‑TTGTTCAAGACTGCCGTCCTGCAAATCGTCACGGAACAGTCGTTCCAGCGCCAGCAAGGATGAAAGCAGCGCAGCAACCCAGATAATGcc                                                                                                                             >  1:91603/1‑211 (MQ=255)                                                                                                                                       aTTCCCAGTAGCATTGCTACCAGTGGCGAAAGGATGAGTAACGGCAGACCGGTTACCATCCAGTGCGCCATCACCTTCGCCAGCACAACGGCGGGCAAGGGTAACGGCAACAACATCAA‑TTGTTCAAGACTGCCGTCCTGCAAATCGTCACGGAACAGTCGTTCCAGCGCCAGCAAGGATGAAAGCAGCGCAGCAACCCAGATAATGCCCGGTGCAATACGCGCCAGCAGTTGCGGCTc                                                                                               <  1:1029910/239‑1 (MQ=255)                                                                                                                                         tCCCAGTAGCATTGCTACCAGTGGCGAAAGGATGAGTAACGGCAGACCGGTTACCATCCAGTGCGCCATCACCTTCGCCAGCACAACGGCGGGCAAGGGTAACGGCAACAACATCAA‑TTGTTCAAGACTGCCGTCCTGCAAATCGTCACGGAACAGTCGTTCCAGCGCCAGCAAGGATGAAAGCAGCGCAGCAACCCAGATAATGCCCGGTGCAATACGCGCCAGCAGTTGCGGCTCCg                                                                                             <  1:1248906/239‑1 (MQ=255)                                                                                                                                                aGCATTGCTACCAGTGGCGAAAGGATGAGTAACGGCAGACCGGTTACCATCCAGTGCGCCATCACCTTCGCCAGCACAACGGCGGGCAAGGGTAACGGCAACAACATCAA‑TTGTTCAAGACTGCCGTCCTGCAAATCGTCACGGAACAGTCGTTCCAGCGCCAGCAAGGATGAAAGCAGCGCAGCAACCCAGATAATGCCCGGTGCAATACGCGCCAGCAGTTGCGGCTCCGGA‑c                                                                                         <  2:30512/235‑1 (MQ=255)                                                                                                                                                  cATTGCTACCAGTGGCGAAAGGATGAGTAACGGCAGACCGGTTACCATCCAGTGCGCCATCACCTTCGCCAGCACAACGGCGGGCAAGGGTAACGGCAACAACATCAA‑TTGTTCAAGACTg                                                                                                                                                                                                          <  2:273141/121‑1 (MQ=255)                                                                                                                                                  cATTGCTACCAGTGGCGAAAGGATGAGTAACGGCAGACCGGTTACCATCCAGTGCGCCATCACCTTCGCCAGCACAACGGCGGGCAAGGGGAACGGCAACAACATCAA‑TTGTTCAAGACTg                                                                                                                                                                                                          >  1:273141/1‑121 (MQ=255)                                                                                                                                                   aTTGCTACCAGTGGCGAAAGGATGAGTAACGGCAGACCGGTTACCATCCAGTGCGCCATCACCTTCGCCAGCACAACGGCGGGCAAGGGTAACGGCAACAACATCAA‑TTGTTCAAGACTGCCGTCCTGCAAATCGTCACGGAACAGTCGTTCCAGCGCCAGCAAGGATGAAAGCAGCGCAGCAACCCAGATAATGCCCGGTGCAATACGCGCCAGCAGTTGCGGCTCCGGA‑CCGATa                                                                                    <  1:114605/237‑1 (MQ=255)                                                                                                                                                     tGCTACCAGTGGCGAAAGGATGAGTAACGGCAGACCGGTTACCATCCAGTGCGCCATCACCTTCGCCAGCACAACGGCGGGGAAGGGGAACGGCAACAACATCAA‑TTGTTCAAGACTGCCCTCCTGCAAATCGTCACGGGACAGACGTTCCAGCGCCAGCAAGGATGAAAGCAACGCAGCAAACCAAAAAAATCCCCGGGGAAAACGCGCCAGCACTTGCGGGCCCCGA‑cccacacgcg                                                                                >  2:1371676/1‑231 (MQ=255)                                                                                                                                                     tGCTACCAGTGGCGAAAGGATGAGTAACGGCAGACCGGTTACCATCCAGTGCGCCATCACCTTCGCCAGCACAACGGCGGGCAAGGGTAACGGCAACAACATCAA‑TTGTTCAAGACTGCCGTCCTGCAAATCGTCACGGAACAGTCGTTCCAGCGCCAGCa                                                                                                                                                               <  1:383294/161‑1 (MQ=255)                                                                                                                                                      gCTACCAGTGGCGAAAGGATGAGTAACGGCAGACCGGTTACCATCCAGTGCGCCATCACCTTCGCCAGCACAACGGCGGGCAAGGGTAACGGCAACAACATCAA‑TTGTTCAAGACTGCCGTCCTGCAAATCGTCACGGAACAGTCGTTCCAGCGCCAGCAAGGATGAAAGCAGCGCAGCAACCCAGATAATGCCCGGTGCAATACGCGCCAGCAGTTGCGGCTCCGGA‑CCGATACTGAg                                                                               <  2:539122/239‑1 (MQ=255)                                                                                                                                                             gTGGCGAAAGGATGAGTAACGGCAGACCGGTTACCATCCAGTGCGCCATCACCTTCGCCAGCACAACGGCGGGCAAGGGGAAAGGCCACAACACCAA‑ATTTTCAAGAACGCCGTCCTGCAAAACGGCACGGAACAGTCGTTCCAGCGCCAGCAAGGAGGAAAGCAGCGCAGCAACCCAGAAAAATCCCCGGGCAAAACGGGCCCACCGTTGGGGGCCCGGA‑CCGAAACTGCGGGGgaaa                                                                        >  2:1453611/1‑239 (MQ=255)                                                                                                                                                              tGGCGAAAGGATGAGTAACGGCAGACCGGTTACCATCCAGTGCGCCATCACCTTCGCCAGCACAACGGCGGGCAAGGGTAACGGCAACAACATCAA‑TTGTTCAAGACTGCCGTCCTGCa                                                                                                                                                                                                <  1:377907/119‑1 (MQ=255)                                                                                                                                                               ggCGAAAGGATGAGTAACGGCAGACCGGTTACCATCCAGTGCGCCATCACCTTCGCCAGCACAACGGCGGGCAAGGGTAACGGCAACAACATCAA‑TTGTTCAAGACTGCCGTCCTGCAAATCGTCACGGAACAGTCGTTCCAGCGCCAGCAAGGATGAAAGCAGCGCAGCAACCCAGATAATGCCCGGTGCAATACGCGCCAGCAGTTGCGGCTCCGGA‑CCGATACTGAGCGGaaa                                                                         <  1:866366/236‑1 (MQ=255)                                                                                                                                                               ggCGAAAGGATGAGTAACGGCAGACCGGTTACCATCCAGTGCGCCATCACCTTCGCCAGCACAACGGCGGGCAAGGGGAAAGGCAACAACATCAA‑TTGTTCAAGAATGCCGGCCTGCAAATCGTCACGGAAAAGTCGTTCCAGCGCCAGCAAGGATGaaaa                                                                                                                                                     >  2:383294/1‑160 (MQ=255)                                                                                                                                                                  gAAAGGATGAGTAACGGCAGACCGGTTACCATCCAGTGCGCCATCACCTTCGCCAGCACAACGGCGGGCAAGGGTAACGGCAACAACATCAA‑TTGTTCAAGACTGCCGTCCTGCAAATCGTCACGGAACAGTCGTTCCAGCGCCAGCAAGGATGAAAGCAGCGCAGCAACCCAGATAATGCCCGGTGCAATACGCGCCAGCAGTTGCGGCTCCGGA‑CCGATACTGAGCGGAAAAAGGGt                                                                   <  1:859531/239‑1 (MQ=255)                                                                                                                                                                                 ggCAGACCGGTTACCATCCAGTGCGCCATCACCTTCGCCAGCACAACGGCGGGCAAGGGTAACGGCAACAACATCAA‑TTGTTCAAGACTGCCGTCCTGCAAATCGTCACGGAACAGTCGTTCCAGCGCCAGCAAGGATGAAAGCAGCGCAGCAACCCAGATAATGCCCGGTGCAATACGCGCCAGCAGTTGCGGCTCCGGA‑c                                                                                         >  1:1373632/1‑202 (MQ=255)                                                                                                                                                                                 ggCAGACCGGTTACCATCCAGTGCGCCATCACCTTCGCCAGCACAACGGCGGGCAAGGGTAACGGCAACAACATCAA‑TTGTTCAAGACTGCCGTCCTGCAAATCGTCACGGAACAGTCGTTCCAGCGCCAGCAAGGATGAAAGCAGCGCAGCAACCCAGATAATGCCCGGTGCAATACGCGCCAGCAGTTGCGGCTCCGGA‑c                                                                                         <  2:1373632/202‑1 (MQ=255)                                                                                                                                                                                  gCAGACCGGTTACCATCCAGTGCGCCATCACCTTCGCCAGCACAACGGCGGGCAAGGGGAACGGCCACAACATCAA‑TTTTTCAAGACCGCCGTCCCGCAAATCGTCCCCGAACAGTCGTTCCAGCGCCAGCAAGGGTGAAAGCAGCGGAGGAACCCCGATAATGCCCGGGGCAATAAGCGCCAGCAGCTGGGGGCCCGGACCCGAAACGGGGGGGAAAAAGGG‑AATTTCAAACAGggag                                                   >  2:1038862/1‑239 (MQ=255)                                                                                                                                                                                      aCCGGTTACCATCCAGTGCGCCATCACCTTCGCCAGCACAACGGCGGGCAAGGGTAACGGCAACAACATCAA‑TTGTTCAAGACTGCCGTCCTGCAAAt                                                                                                                                                                                             <  1:337088/98‑1 (MQ=255)                                                                                                                                                                                         ggTTACCATCCAGTGCGCCATCACCTTCGCCAGCACAACGGCGGGCAAGGGTAACGGCAACAACATCAA‑TTGTTCAAGACTGCCGTCCTGCAAATCGTCACGGAACAGTCGTTCCAGCGCCAGCAAGGATGAAAGCAGCGCAGCAACCCAGATAATGCCCGGTGCAATACGCGCCAGCAGTTGCGGCTCCGGA‑CCGATACTGAGCGGAAAAAGGGTAATTACAATCAGGAAGAACcaca                                            <  1:237633/239‑1 (MQ=255)                                                                                                                                                                                                aTCCAGTGCGCCATCACCTTCGCCAGCACAACGGCGGGCAAGGGGAACGGCCACAACATCAA‑TTTTTCCAGACTGCCGTCCTGCAAATCGTCACGGaa                                                                                                                                                                                   >  2:337088/1‑98 (MQ=255)                                                                                                                                                                                                aTCCAGTGCGCCATCACCTTCGCCAGCACAACGGCGGGCAAGGGGAACGGCCACAACATCAA‑TTGTTCAAGACTGCCGTCCTGCCAATCGTCCCGGAACAGTCGTTCCCGCGCCAGCAAGGCTGAAAACAGCGCAGCAACCCAGATAATGACCCGTGCAATAAGCGCCAGCAAGTGCGGCCCCGGA‑CCGATACTGAGCGGAAAAAGGGGAATTACAAACAGGACAAACCACAGCgg                                        >  2:11572/1‑236 (MQ=255)                                                                                                                                                                                                    aGTGCGCCATCACCTTCGCCAGCACAACGGCGGGCAAGGGGAAAGGCCACAACAACAA‑TTGTTGAAAAACGCCGTCCTGGCAAACGTCACGGAACAGTACTTTCTGCGGCAACCAGGATGAAAGCAGGACCGCAACCCCGAAAATTCCCGGGGCAATAGGACCCACCAGGTGCCGCTCCGGC‑CCGATCCGCGGCGGAAAAAAGGGACTTATAATACGGGAGAGCCACATCGGGtggtgg                                 >  2:49056/1‑234 (MQ=255)                                                                                                                                                                                                     gTGCGCCATCACCTTCGCCAGCACAACGGCGGGCAAGGGTAACGGCAACAACATCAA‑TTGTTCAAGACTGCCGTCCTGCAAATCGTCACGGAACAGTCGTTc                                                                                                                                                                          >  1:1250421/1‑102 (MQ=255)                                                                                                                                                                                                     gTGCGCCATCACCTTCGCCAGCACAACGGCGGGCAAGGGTAACGGCAACAACATCAA‑TTGTTCAAGACTGCCGTCCTGCAAATCGTCACGGAACAGTCGTTc                                                                                                                                                                          <  2:1250421/102‑1 (MQ=255)                                                                                                                                                                                                     gTGCGCCATCACCTTCGCCAGCACAACGGCGGGCAAGGGTAACGGCAACAACATCAA‑TTGTTCAAGACTGCCGTCCTGCAAATCGTCACGGAACAGTCGTTCCAGCGCCAGCAAGGATGAAAGCAGCGCAGCAAccc                                                                                                                                       <  2:709219/137‑1 (MQ=255)                                                                                                                                                                                                     gTGCGCCATCACCTTCGCCAGCACAACGGCGGGCAAGGGTAACGGCAACAACATCAA‑TTGTTCAAGACTGCCGTCCTGCAAATCGTCACGGAACAGTCGTTCCAGCGCCAGCAAGGATGAAAGCAGCGCAGCAAccc                                                                                                                                       >  1:709219/1‑137 (MQ=255)                                                                                                                                                                                                     gTGCGCCATCACCTTCGCCAGCACAACGGCGGGCAAGGGGAACGGCAACAACATCAA‑TTGTTCAAGACTGCCGTCCTGCAAAACGGCCCGGAAAAGTCGTTCCAGCGCCAGCAAGGAGGAAAACAGCGCAGCAACCCCGATAATACCCCGGGCAAAACGCGCCAGCACTTGCGGCCCCGGG‑CCGATACTGAGCGGGAAAAGGGGAATTACAAACAGGAAAAAACCAAACGGGTTgtgga                                >  2:20996/1‑235 (MQ=255)                                                                                                                                                                                                               aCCTTCGCCAGCACAACGGCGGGCAAGGGTAACGGCAACAACATCAA‑TTGTTCAAGACTGCCGTCCTGCAAATCGTCACGGAACAGTCGTTCCAGCGCCAGCAAGGATGAAAGCAGCGCAGCAACCCAGATAATGCCCGGTGCAATACGCGCCAGCAGTTGCGGCTCCGGA‑CCGATACTGAGCGGAAAAAGGGTAATTACAATCAGGAAGAACCACAGCGGGTTGGCGATTTCGGCGCt                      <  2:1197339/239‑1 (MQ=255)                                                                                                                                                                                                                     gCCAGCACAACGGCGGGCAAGGGTAACGGCAACAACATCAA‑TTGTTCAAGACTGCCGTCCTGCAAATCGt                                                                                                                                                                                          >  2:1257607/1‑70 (MQ=255)                                                                                                                                                                                                                     gCCAGCACAACGGCGGGCAAGGGTAACGGCAACAACATCAA‑TTGTTCAAGACTGCCGTCCTGCAAATCGt                                                                                                                                                                                          <  1:1257607/70‑1 (MQ=255)                                                                                                                                                                                                                     gCCAGCACAACGGCGGGCAAGGGTAACGGCAACAACATCAA‑TTGTTCAAGACTGCCGTCCTGCAAATCGTCACGGAACAGTCGTTCCAGCGCCAGCAAGGATGAAAGCAGCGCAGCAACCCAGATAATGCCCGGTGCAATACGCGCCAGCAGTTGCGGCTCCGGA‑CCGATACTGAGCGGAAAAAGGGTAATTACAATCAGGAAGAACCACAGCGGGTTGGCGATTTCGGCGCTATGGCg                <  1:16156/239‑1 (MQ=255)                                                                                                                                                                                                                                ggcggGCAAGGGTAACGGCAACAACATCAA‑TTGTTCAAGACTGCCGTCCTGCAAATCGTCACGGAACAGTCGTTCCAGCGCCAGCAAGGATGAAAGCAGCGCAGCAACCCAGATAATGCCCGGTGCAATACGCGCCAGCAGTTGCGGCTCCGGA‑CCGATACTGAGCGGAAAAAGGGTAATTACAATCAGGAAGAACCACAGCGGGTTGGCGATTTCGGCGCTATGGCGAAACGCt         <  1:94724/235‑1 (MQ=255)                                                                                                                                                                                                                                   gggCAAGGGTAACGGCAACAACATCAA‑TTGTTCAAGACTGCCGTCCTGCAAATCGTCACGGAACAGTCGTTCCAGCGCCAGCAAGGATGAAAGCAGCGCAGCAACCCAGATAATGCCCGGTGCAATACGCGCCAGCAGTTGCGGCTCCGGA‑CCGATACTGAGCGGAAAAAGGGTAATTACAATCAGGAAGAACCACAGCGGGTTGGCGATTTCGGCGCTATGGCGAAACGCTaca      <  1:1127635/235‑1 (MQ=255)                                                                                                                                                                                                                                    ggCAAGGGGAACGGCAAAAACATCAA‑TTGTTCAAGACTGCCGTCCTGCAAAACGGCACGGAACAGGCGTTCCAGCGCCAGCAAGGATGAAAGCAGCGCAGCAACCCAGATAAAGCCCGGGGCAATAAGCGCCAGCAGGTGCGGCTCCGGA‑CCGCAACTGAGCGGAAAAAGGGGAATTACAATCAGGAAGAACCACAGAGGGGTGGCGATTTCGGCGCGATGGCGAAACGCTACACGCa  >  2:219457/1‑238 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                        ACACCAGTATGCTGAGCAGCACACCACCGCGCTTAAGTCCCACTGTCAGCGCCACGCCCGGTGCACCGAGAAAGCCAAGCGTAGGCGTTCCCAGCAGCAGCGTCAGCGCCATCACTTGCCAGCCATAAACATCCATTCCCAGTAGCATTGCTACCAGTGGCGAAAGGATGAGTAACGGCAGACCGGTTACCATCCAGTGCGCCATCACCTTCGCCAGCACAACGGCGGGCAAGGGTAACGGCAACAACATCAA‑TTGTTCAAGACTGCCGTCCTGCAAATCGTCACGGAACAGTCGTTCCAGCGCCAGCAAGGATGAAAGCAGCGCAGCAACCCAGATAATGCCCGGTGCAATACGCGCCAGCAGTTGCGGCTCCGGA‑CCGATACTGAGCGGAAAAAGGGTAATTACAATCAGGAAGAACCACAGCGGGTTGGCGATTTCGGCGCTATGGCGAAACGCTACACGCA  >  NC_000913/2296529‑2296993

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑