| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,828,890 | C→A | 6.2% | R282L (CGT→CTT) | astD ← | succinylglutamic semialdehyde dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,828,890 | 0 | C | A | 6.2% | 222.3 / 4.4 | 65 | R282L (CGT→CTT) | astD | succinylglutamic semialdehyde dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base C (31/30); new base A (2/2); total (33/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.17e-01 | |||||||||||
ACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGG‑GTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAG‑CCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATCGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTCAGCAATAAACGGC‑GGGCGCAGGTGCAGCGTTGACCGGCTGTGACAAACGCCGACTGAATGGTCAGATGGACAGCCGCGTCGATATCCGCCACCTCATCGATAATTAGCGGATTATTACCGCCCATCTCAAGGGCGAGAATTTTCTCCGGCTGACCGGAGAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTAAACAGCAAACCGTCGAGATCCTCCAGCGCACT > NC_000913/1828666‑1829113 | acgtgttCCCTGGTTGAAAT‑GGCCCGGCGCAAACGGGGTGTCGCCCCCCCATGCCTCCGAGTCGCTGCCATGACGAACCCCCCCGCTG‑GCGGGCCTTTCAAAAACCCGCCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATCGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTCAGCAATAAAcggc < 2:259192/222‑1 (MQ=255) gtgatttGAAATAGCCGGGGCGAAGGCGGGG‑GCCCTCCTCCCATCGCTCCCAGTTGCTGCCATGCAATCACCCCCTGTGGTGCGGCTTGTTCAGAAATCAG‑CCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATCGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTCAGCAATAAACggc‑gggcg < 2:1270919/220‑1 (MQ=255) gCTTGTAATAAGCGCGGCGCAAGCAGGG‑GTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAG‑CCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATCGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTCAGCAATAAACggc‑ggggcccgggg > 1:1028527/1‑220 (MQ=255) cTTGTAATAAGCGCGGCGCAAGCAGGG‑GTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGCAACCACCTGCTGTGCGGCCTGTTCAGAAATCAG‑CCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATCGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTCAGCAATAACCGGC‑GGGCGAAGGAGc > 1:460374/1‑228 (MQ=255) gcgcGGCGCAAGCAGGG‑GTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAG‑CCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATCGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTCAGCAATAAACGGC‑GGGCCGACGTGGAGCGGTGAcc > 2:155324/1‑228 (MQ=255) ggcgCAAGCAGGG‑GTCGTCCGCCCATCGCTTCCATTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAG‑CCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATTGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTCAGCAATAAACGGC‑GGGCGCAGGTGCAGCGTTCACCGGc > 1:1727427/1‑227 (MQ=255) cccATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAG‑CCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATCGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTCAGCAATAAACGGC‑GGGGGCAGGGGCAGCGTTGGACGGCTGTGGCAAAAGCCGCATGaaag > 2:1626665/1‑226 (MQ=255) cccATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAG‑ACCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATCGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTCAGCAATAAACGGC‑GGGGGCACGGGCAGCGTTTACCGGCGTTGTCAAACGcccaatgtaag > 2:1182988/1‑219 (MQ=255) atttGCGGCCATGCATTACCCCGCTGCTGTGGCGTCTGTACAGAAATCCC‑CCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATCGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTCAGCAATAAACGGC‑GGGCGCAGGTGCAGCGTTGACCGGCTGTGACAAACGCCGACTGAATGGTCAGATGGACa < 2:1606245/226‑1 (MQ=255) cacccccTGCTGTGCGGCCTGTCCAGAATCCAG‑CCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATCGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTCAGCAATAAACGGC‑GGGCGCAGGTGCAGCGTTGACCGGCTGTGACAAACGCCGACTGAATGGTCAGATGGACAGCCGCGTCGATATCCGc < 1:1629545/227‑1 (MQ=255) accTGCTGTGCGGCCTGTTCAGAAATCAG‑CCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATCGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCGCCGTTTATTGCTGAAAAGCG‑GGGCGCAGGTGCAGCGTTGACCGGCTGTGACAAACGCCGACTGAATGGTCAGATGGACAGCCGCGTCGATATCCGCCAcc > 1:180440/1‑228 (MQ=255) ctgTGCGGCCTGTTCAGAAATCAG‑CCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATCGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTCAGCAATAAACGGC‑GGGCGCAGGTGCAGCGTTGACCGGCTGTGACAAACGCCGACTGAATGGt > 1:130165/1‑192 (MQ=255) gtgggcgCCTTTTAAAAAAACAGCCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATCGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTCAGCAATAAACGGC‑GGGCGCAGGTGCAGCGTTGACCGGCTGTGACAAACGCCGACTGAATGGTc < 2:129934/187‑1 (MQ=255) tgCGGCCTGTTCAGAAATCAG‑CCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATCGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTCAGCAATAAACGGC‑GGGCGCAGGTGCAGCGTTCACCGGCTGTGCCCAACGCCGACTGAAAGGTCAGATGGCCAGCCGCGCCGCAATCCGCCACCTCCTCgct > 1:699399/1‑226 (MQ=255) gCGGCCTGTCCAGAAAGCAC‑CCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATCGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTCAGCAATAAACGGC‑GGGCGCAGGTGCAGCGTTGACCGGCTGTGACAAACGCCGACTGAATGGTc < 1:1364056/189‑1 (MQ=255) cGGCCTGTTCAGAAATCAG‑CCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATCGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTCAGCAATAAACGGC‑GGGCGCAGGTGCAGCGTTGACCGGCTGTGACAAACGCCGACTGAATGGTCAGGTGGACAGCCGCGTCGATATCCGCCACCTCATCg > 1:1043786/1‑224 (MQ=255) cGGCCTGTTCAGAAATCAG‑CCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATCGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTCAGCAATAAACGGC‑GGGCGCAGGGGCAGCGTTGACCGGCTGTGACAAACGCCGACTGAATGGGCAGATGGACAGCCGCGTCGATATCCGCCACCCCATCGCTaa > 1:1132050/1‑228 (MQ=255) gCCTGTTCAGAAATCAG‑CCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATCGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTCAGCAATAAACGGC‑GGGCGCAGGGGCAGCGTTGACCGGCTGTGGCAAACGCCGACTGAATGGTCAGATGGACAGCCGCGTCGATATCCGCCCCCTCATCGATAAtt > 1:228308/1‑228 (MQ=255) aaaTCAG‑CCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATCGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTCAGCAATAAAcggc > 1:610385/1‑126 (MQ=255) aaTCAG‑CCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATCGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTCAGCAATAAAcggc‑g < 2:609391/126‑1 (MQ=255) aG‑CCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATCGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTCAGCAATAAACGGC‑GGGCGCAGGTGCAGCGTTGACCGGCTGTGACAAACGCCGACTg > 2:1476832/1‑164 (MQ=255) aG‑CCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATCGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTCAGCAATAAACGGC‑GGGCGCAGGTGCAGCGTTGACCGGCCGTGACCAACGCCCGCTGa > 2:41450/1‑165 (MQ=255) aG‑CCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATCGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTCAGCAATAAACGGC‑GGGCGCAGGGGCAGCGTTGACCGGCTGTGACAAACGCCGACTGAATGGTCAGATGGGCAGCCGCGTCGATATCCGCCACCTCATCGATAATTAACGGATTATTTCCg > 1:44338/1‑228 (MQ=255) g‑CCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATCGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTCAGCAATAAACGGC‑GGGCGCAGGTGCAGCGTTGACCGGCTGTGACAAACGCCGACTGaa < 1:41523/165‑1 (MQ=255) agaccGCCAATAAACGGCTGCGGTTCGTCATCAAAGTTGCCCGGCGTTAATCGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTCAGCAATAAACGGC‑GGGCGCAGGTGCAGCGTTGACCGGCTGGGACAAAAGCCGACTGAATGGTCAGATGGAAAGCACGGGCGAGATCCGCGACCGCATCGAGTATTTGCGGATTATTACCg > 2:1111894/4‑228 (MQ=255) cccGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCGGGCGTTAATCGCTTGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCTTGTCAGCAATAAACGGC‑GGGCGCAGGTGCAGCGTTGACCGGCTGTGACAAACGCCGACTGAATGGTCAGATGGACAGCCGCGTCGATATCCGCCACCTCATCGATAATTAGCGGATTATTACCGcc < 1:1956441/228‑1 (MQ=255) ccAATAAGCGGCATCAGTTCGGCATCCCAGTTGCCCGGCGTTAACCGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTCAGCAATAAACGGC‑GGGCGCAGGTGCAGCGTTGACCGGCTGTGACAAACGCCGACTGAATGGTCAGATGGACAGCCGCGTCGATATCCGCCACCTCATCGATAATTAGCGGATTATTACCGcc < 2:312631/224‑1 (MQ=255) ggCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATCGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTCAGCAATAAACGGC‑GGGCGCAGGTGCAGCGTTGACCGGCTGTGACAAACGCCGACTGAATGGTCAGATGGACAGCCGCGTCGATATCCGCCACCTCATCGATAATTAGCGGATTATTACCGCCCATCTCAAGGGCg < 2:45072/228‑1 (MQ=255) tGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATCGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTCAGCAATAAACGGC‑GGGCGCAGGGGCAGCGTTGACCGGCTGTGACAAAAGCCGACTGAAAGGGCAGATGGGCAGCCGCGTCGACATACGCCCCCCCCACGGTAAATAGCGGGGTATTAATCCCCCTATCCAGGGCggga > 2:1274784/1‑228 (MQ=255) cccAGTTGCCCGGCGTTAATCGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTCAGCAATAAACGGC‑GGGCGCAGGTGCAGCGTTGACCGGCTGTGACAAACGCCGACTGAAAGGTCAGATGGGCAGCCGCGTCGATATCCGCCACCCCATCGATAATTTGCgg > 2:443122/1‑187 (MQ=255) ccAGTTGCCCGGCGTTAATCGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTCAGCAATAAACGGC‑GGGCGCAGGTGCAGCGTTGACCGGCTGTGACAAACGCCGACTGAATGGTCAGATGGACAGCCGCGTCGATATCCGCCACCTCATCGATAATTAGCGGa < 1:443853/187‑1 (MQ=255) gCCCGGCGTTAATCGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCGCCGTTTATTGCTGAAAAGCG‑GGGCGCAGGTGCAGCGTTGACCGGCTGTGACAAACGCCGACTGAATGGTCAGATGGACAGCCGCGTCGATATCCGCCACCTCATCGATAATTAGCGGATTCTTACCGCCCATCTCAAGGGCGAGAATTTTCTCCGGCTGTCCGga > 1:1195394/1‑228 (MQ=255) ggCGTTAATCGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTCAGCAATAAACGGC‑GGGCGCAGGTGCAGCGTTGACCGGCTGTGACAAACGcc > 2:371786/1‑117 (MQ=255) gctttagccGCTGGCTGAGGGCAGCAAGACGAGCAAGAAACGCTCCGCCCTGCGCCCGCCGTTTATTGCTGAAAAGCG‑GGGCGCAGGGGCAGCGTTGACCGGCTGTGACAAACGCCGACTGAATGGTCAGATGGACAGCCGCGTCGATATCCGCCACCTCATCGATAATTAGCGGATTATTACCGCCCATCTCAAGGGCGAGAATTTTCTCCGGCTGACCGGAGAGCt < 2:1193252/220‑1 (MQ=255) gCGTTAATCGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTCAGCAATAAACGGC‑GGGCGCAGGTGCAGCGTTGACCGGCTGTGACAAACGCCg < 1:372403/117‑1 (MQ=255) gCGTGAATCGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTCAGCAATAAACGGC‑GGGCGCAGGTGCAGCGTTGACCGGCTGTGACAAACGCCGACTGAATGGTCAGATGGACAGCCGCGTCGATATCCGCCACCTCATCGATAATTAGCGGATTATTACCGCCCATCTCAAGGGCGAGAATTTTCTCCGGCTGACCGGAGAGCt < 1:600804/228‑1 (MQ=255) cGTTAATCGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTCAGCAATAAACGGC‑GGGCGCAGGTGCAGCGTTGACCGGCTGTGACAAACGCCGACTGAATGGTCAGATGGACAGCCGCGTCGATATCCGCCACCTCATCGATAATTAGCGGATTATTACCGCCCATCTCAAGGGCGAGAATTTTCTCCGGCTGACCGGagag > 2:468406/1‑225 (MQ=255) accggttgctgACCGCAACCAGCCGAGCAAGAAACGCCTCGCCCTGCGCCCCGCTTTTCAGCAATAAACGGC‑GGGCGCAGGTGCAGCGTTGACCGGCTGTGACAAACGCCGACTGAATGGTCAGATGGACAGCCGCGTCGATATCCGCCACCTCATCGATAATTAGCGGATTATTACCGCCCATCTCAAGGGCGAGAATTTTCTCCGGCTGACCGGAGAGCTGGCGAt < 2:1026660/221‑1 (MQ=255) tCGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTCAGCAATAAACGGC‑GGGCGCAGGTGCAGCGTTGACCGGCTGGGACAAACGCCGACTGAATGGTCAGATGGACAGCCGCGTCGATATCCGCCCCCTCATCGGTAATTAATGGGTTATTACCGCCCAACCCAAGGGCGAGAAATTTCTCCCGCTGCCCGgagggacggccgg > 2:1868317/1‑217 (MQ=255) tggctgACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTCAGCAATAAACGGC‑GGGCGCAGGGGCAGCGTTGTCCCGCTTGTACAAACGCCGACTGGATGGTCAGATGGCCAGCCGCGTCGGTATACGCCCCCCCCTCGATAATTACCGGGTTATTACCGCCCATCTCCAGCGGTGGAATTTTCTCCGGCCGACCGGCGAAGTGGCGATg > 2:1429892/1‑224 (MQ=255) gctgACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTCAGCAATAAACGGC‑GGGCGCAGGTGCAGCGTTGACCg > 1:508464/1‑88 (MQ=255) gctgACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTCAGCAATAAACGGC‑GGGCGCAGGTGCAGCGTTGACCGGCTGTGACAAACGCCGACTGa > 1:1683429/1‑109 (MQ=255) ctgACGGCAACCAGACGAGCAGGAAACGCATCGCCCTGCGCCCCGCTTTTCAGCAATAAACGGC‑GGGCGCAGGTGCAGCGTTGACCgg < 2:507634/88‑1 (MQ=255) ctgACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTCAGCAATAAACGGC‑GGGCGCAGGTGCAGCGTTGACCGGCTGTGACAAACGCCGACTGaa < 2:1680459/109‑1 (MQ=255) gAGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTCAGCAATAAACGGC‑GGGCGCAGGTGCAGCGTTGACCGGCTGTGACAAACGCCGACTGAATGGTCAGATGGACAGCCGCGTCGATATCCGCCACCTCATCGATAATTAGCGGATTATTACCGCCCATCTCAAGGGCGAGAATTTTCTCCGGCTGACCGGAGAGCTGGCGATGCAACTGGTAGCCTGTATTGgcgc < 1:469173/228‑1 (MQ=255) gAGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTCAGCAATAAACGGC‑GGGCGCAGGTGCAGCGTTGACCGGCTGTGACAAACGCCGACTGAATGGTCAGATGGACAGCCGCGTCGATATCCGCCACCTCATCGATAATTAGCGGATTATTACCGCCCATCTCAAGGGCGAGAATTTTCTCCGGCTGACCGGAGAGCTGGCGATGCAACTGGTAGCCTGTATTGgcgc < 2:1724374/228‑1 (MQ=255) aGCAGGAACCGCATCGCCCTGC‑CCCCGCCGTTTATTGCTGAAAAGCGGGGCGCAGGTGCAGCGTTGACCGGCTGTGACAAACGCCGACTGAATGGTCAGATGGACAGCCGCGTCGATATCCGCCACCTCATCGATAATTAGCGGATTATTACCGCCCATCTCAAGGGCGAGAATTTTCTCCGGCTGACCGGAGAGCTGGCGATGCAACTGGTAGCCTGTATTGgcgc < 2:180129/227‑1 (MQ=255) cAAGAAACGCATCGCCCTGCGCCCCGCTTTTCAGCAATAAACGGC‑GGGCGCAGGTGCAGCGTTGACCGGCTGTGACAAACGCCGACTGAATGGTCAGATGGACAGCCGCGTCGATATCCGCCACCTCATCGATAATTAGCGGATTATTACCGCCCATCTCAAGGGCGAGAATTTTCTCCGGCTGACCGGAGAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTAc < 1:1564088/228‑1 (MQ=255) atcGCATCGCCCTGCGCCCCGCTGTTCAGCAATAAACGGC‑GGGCGCAGGTGCAGCGTTGACCGGCTGTGACAAACGCCGACTGAATGGTCAGATGGACAGCCGCGTCGATATCCGCCACCTCATCGATAATTAGCGGATTATTACCGCCCATCTCAAGGGCGAGAATTTTCTCCGGCTGACCGGAGAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTa < 1:411596/226‑1 (MQ=255) ccGCATCGCCCTGCGCCCCGCTTTTCAGCAATAAACGGC‑GGGCGCAGGTGCAGCGTTGACCGGCTGTGACAAACGCCGACTGAATGGTCAGATGGACAGCCGCGTCGATATCCGCCACCTCATCGATAATTAGCGGATTATTACCGCCCATCTCAAGGGCGAGAATTTTCTCCGGCTGACCGGAGAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTa < 1:1871624/220‑1 (MQ=255) cgctgcaccTGCGCCCCGCTTTTCAGCAATAAACGGC‑GGGCGCAGGTGCAGCGTTGACCGGCTGTGACAAACGCCGACTGAATGGGCAGATGGACAGCCGCGGCGATATCCGCCACCTCATCGATAATTAGCGGATTATTACCGCCCATCTCAAGGGCGAGAAATTTCTCCGGCTGACCGGAGAGATGACCATGCAACTGGTGGCCTGGATTGTCGCTACCGGTAAAc > 2:1449871/8‑228 (MQ=255) gcgcCCCGCTTTTCAGCAATAAACGGC‑GGGCGCAGGTGCAGCGTTGACCGGCTGGGACAAACGCCGACTGAATGGGCAGATGGACAGCCGCGTCGATATCCGCCACCTCATCGATAATTAGCGGATTATTACCGCCCATCTCAAGGGCGCGAATTTTCTCCGGCTGACCGGAGAGCTGGCGATTCAAATGGTAGCCCGTATTGGGGCTACCCGGAAACAGCGAACCgg > 2:1057442/1‑227 (MQ=255) gcCCCGCTTTTCAGCAATAAACGGC‑GGGCGCAGGTGCAGCGTTGACCGGCTGTGACAAACGCCGACTGAATGGTCAGATGGACAGCCGCGTCGATATCCGCCACCTCATCGATAATTAGCGGATTATTACCGCCCATCTCAAGGGCGAGAATTTTCTCCGGCTGACCGGAGAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTAAACAGCAAACCGTCg < 1:1277067/228‑1 (MQ=255) ccccGCTTTTCAGCAATAAACGGC‑GGGCGCAGGTGCAGCGTTGACCGGCTGTGACAAACGCCGACTGAATGGTCAGATGGACAGCCGCGTCGATATCCGCCACCTCATCGATAATTAGCGGATTATTACCGCCCATCTCAAGGGCGAGAATTTTCTCCGGCTGACCGGAGAGCTGGCGATGCAACAGGGAGCCTGGATTGGGGCTACCGGGAAACAGCAAACCGTCg > 1:97872/1‑227 (MQ=255) ccccGCTTTTCAGCAATAAACGGC‑GGGCGCAGGGGCAGCGTTGACCGGCTGTGACAAACGCCCACTGAATGGTCAGATGGACAGCCGCGTCGATATCCCCCACCCCATCGATAAATa > 2:597180/1‑117 (MQ=255) ccccGATTTTCAGCAATAAACGGC‑GGGCGCAGGTGCAGCGTTGACCGGCTGTGACAAACGCCGACTGAATGGTCAGATGGACAGCCGCGTCGATATCCGCCCCCTCATCGATAATTAGCGGATTATTACCGCCCATCTCAAGGGCGAGAATTTTCTCCGGCTGACCGGAGAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTAAACAGCAAACCGTCg < 1:348702/227‑1 (MQ=255) cccGCTTTTCAGCAGTGAGCGGC‑GGGGGGGGGTGCAACGTTGGCGGGAGGTGACAAACGAGGTCTGAATGGTCAGGTGGACAGCCGCGTCGATATCCGCCACCTCATCGATAATTAg < 1:598159/117‑1 (MQ=255) ccGCTTTTCAGCAATAAACGGC‑GGGCGCAGGTGCAGCGTTGACCGGCTGTGACAAACGCCGACTGAATGGTCAGATGGACAGCCGCGTCGATATCCGCCACCTCATCGATAATTa > 1:55373/1‑115 (MQ=255) cGCTTTTCAGCAATAAACGGC‑GGGCGCAGGTGCAGCGTTGACCGGCTGTGACAAACGCCGACTGAATGGTCAGATGGACAGCCGCGTCGATATCCGCCACCTCATCGATAATTAg < 2:55275/115‑1 (MQ=255) cGCTTTTCAGCAATAAACGGC‑GGGCGCAGGTGCAGCGTTGACCGGCTGTGACAAACGCCGACTGAATGGTCAGATGGACAGCCGCGTCGATATCCGCCACCTCATCGATAATTAGCGGATTATTACCGCCCATCTCCAGGGCGAGAATTTTTTCCGGCTGACCGGAGAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGGGAAAAGCAAACCGTCgcga > 2:363191/1‑227 (MQ=255) gtttcCAGCAATAAACGGC‑GGGCGCAGGTGCCGCGTTGACCGGCTGTGACAAACGCCGACTGAATGGTCAGATGGACAGCCGCGTCGATATCCGCCACCTCATCGATAATTAGCGGATTATTACCGCCCATCTCAAGGGCGAGAATTTTCTCCGGCTGACCGGAGAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTAAACAGCAAACCGTCGAGAtc < 1:479142/226‑1 (MQ=255) cTTTTCAGCAATAAACGGC‑GGGCGCAGGTGCGGCGTTGACCGGCTGTGACAAACGCCGACTGAATGGTCAGATGGACAGCCGCGTCGATATCCGACACCTCATCGATAATTAGCGGATTATTACCGCCCATCTCAAGGGCGAGAATTTTCTCCGGCTGACCGGAGAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTAAACAGCAAACCGTCGAGAtc < 1:1740936/227‑1 (MQ=255) ttttCAGAAATAAACGGC‑GGGCGCAGGTGCAGCGTTGACCGGCTGTGACAAACGCCGACTGAATGGTCAGATGGACAGCCGCGTCGATATCCGCCACCTCATCGATAATTAGCGGATTATTACCGCCCATCTCAAGGGCGAGAATTTTCTCCGGCTGACCGGAGAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTAAACAGCAAACCGTCGAGAtcct < 2:698228/228‑1 (MQ=255) ttCAGCAATAAACGGC‑GGGCGCAGGTGCAGCGTTTACCGGCTGTGACAAACGCCGACTGAATGGTCAGATGGACAGCCGCGTCGATATCCGCCACCTCATCGATAATTAGCGGATTATTACCGCCCATCTCAAGGGCGAGAATTTTCTCCGGCTGACCGGAGAGCTGGCGATGCAACTGGTAg > 2:54621/1‑183 (MQ=255) tCAGCAATAAACGGC‑GGGCGCAGGTGCAGCGTTGACCGGCTGTGACAAACGCCGACTGAATGGTCAGATGGACAGCCGCGTCGATATCCGCCACCTCATCGATAATTAGCGGATTATTACCGCCCATCTCAAGGGCGAGAATTTTCTCCGGCTGACCGGAGAGCTGGCGATGCAACTGGTAGc < 1:54717/183‑1 (MQ=255) tAAACGGC‑GGGCGCAGGTGCAGCGTTGACCGGCTGTGACAAACGCCGACTGAATGGTCAGATGGACAGCCGCGTCGATATCCGCCACCTCATCGATAATTa > 1:1539171/1‑101 (MQ=255) tAAACGGC‑GGGCGCAGGTGCAGCGTTGACCGGCTGTGACAAACGCCGACTGAATGGTCAGATGGACAGCCGCGTCGATATCCGCCACCTCATCGATAATTAGCGGATTATTACCGCCCATCTCAAGGGCGAGAATTTTCTCCGGCTGACCGGAGAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTAAACAGCAAACCGTCGAGATCCTCCAGCGCACt < 2:936185/228‑1 (MQ=255) aaaCGGC‑GGGCGCAGGTGCAGCGTTGACCGGCTGTGACAAACGCCGACTGAATGGTCAGATGGACAGCCGCGTCGATATCCGCCACCTCATCGATAATTAg < 2:1536431/101‑1 (MQ=255) | ACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGG‑GTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAG‑CCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATCGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTCAGCAATAAACGGC‑GGGCGCAGGTGCAGCGTTGACCGGCTGTGACAAACGCCGACTGAATGGTCAGATGGACAGCCGCGTCGATATCCGCCACCTCATCGATAATTAGCGGATTATTACCGCCCATCTCAAGGGCGAGAATTTTCTCCGGCTGACCGGAGAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTAAACAGCAAACCGTCGAGATCCTCCAGCGCACT > NC_000913/1828666‑1829113 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |