| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,921,544 | T→G | 17.6% | V441G (GTA→GGA) | rsmF → | 16S rRNA m(5)C1407 methyltransferase, SAM‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,921,544 | 0 | T | G | 17.6% | 188.7 / 4.4 | 85 | V441G (GTA→GGA) | rsmF | 16S rRNA m(5)C1407 methyltransferase, SAM‑dependent |
| Reads supporting (aligned to +/- strand): ref base T (18/50); new base G (15/2); total (33/52) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.50e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTTCCCGGTGGGCATTGAAGCCCTGATCGGTAAAGTCCGATTTTCTCGGTTGGGGATTAAACTTGCCGAGACGCACAACAAAGGTTATCGCTGGCAGCATGAAGCGGTTATTGCTCTTGCCTCACCCGATAATATGAATGCATTCGAGCTGACACCGCAGGAAGCGGAGGAGTGGTATCGCGGGCGCGATGTTTACCCGCAAGCCGCGCCAGTGGCGGATGATGTATTGGTTACTTTCCA‑GCATCAACCGATTGGTTTAGCCAAACGGATTGGTTCGC‑GATTGAAAAACAGCTATCCGCGTG‑AACT‑GGTGCGCGAT‑GGGAAACTTTTTACCGGTAACGCCTG‑AC‑AGCGCACAAAAAAAGCGCACTTTTTGACTGGCACATTCGGCTGCCTCAACTAGGCTGAAAAATGGTGCGATCGGACTGGTCGTACCACAATCGGCAGCTAAATGGA > NC_000913/1921320‑1921769 | gttCCCGGTGGGCATTGAAGCCCTGATCGGTAAAGTCCGATTTTCTCGGTTGGGGATTAAACTTGCCGAGACGCACAACAAAGGTTATCGCTGGCAGCATGAAGCGGTTATTGCTCTTGCCTCACCCGATAATATGAATGCATTCGAGCTGACACCGCAGGAAGCGGAGGAGTGGTATCGCGGGCGCGATGTTTACCCGCAAGCCGCGCCAGTGGCGGATGATGTAtt > 1:1653450/1‑228 (MQ=255)gttCCCGGTGGGCATTGAAGCCCTGATCGGTAAAGTCCGATTTTCTCGGTTGGGGATTAAACTTGCCGAGACGCACAACAAAGGTTATCGCTGGCAGCATGAAGCGGTTATTGCTCTTGCCTCACCCGATAATATGAATGCATTCGAGCTGACACCGCAGGAAGCGGAGGAGTGGTATCGCGGGCGCGAGGTTTAACCGCAAGCCGAGCCAGTGTCGGGAGATGGAtt > 2:860332/1‑228 (MQ=255) tCCCGGTGGGCATTGAAGCCCTGATCGGTAAAGTCCGATTTTCTCGGTTGGGGATTAAACTTGCCGAGACGCACAACAAAGGTTATCGCTGGCAGCATGAAGCGGTTATTGCTCTTGCCTCACCCGATAATATGAATGCATTCGAGCTGACACCGCAGGAAGCGGAGGAGTGGTATCGCGGGCGCGATGTTTACCCGCAAGCCGCGCCAGTGGCGGATGATGTa < 2:117651/224‑1 (MQ=255) cccGGTGGGCATTGAAGCCCTGATCGGTAAAGTCCGATTTTCTCGGTTGGGGATTAAACTTGCCGAGACGCACAACAAAGGTTATCGCTGGCAGCATGAAGCGGTTATTGCTCTTGCCTCACCCGATAATATGAATGCATTCGAGCTGACACCGCAGGAAGCGGAGGAGTGGTATCGCGGGCGCGATGTTTACCCGCAAGCCGCGCCAGTGGCGGATGATGTATTGGt > 1:269479/1‑228 (MQ=255) cGGTGGGCATTGAAGCCCTGATCGGTAAAGTCCGATTTTCTCGGTTGGGGATTAAACTTGCCGAGACGCACAACAAAGGTTATCGCTGGCAGCATGAAGCGGTTATTGCTCTTGCCTCACCCGATAATATGAATGCATTCGAGCTGACACCGCAGGAAGCGGAGGAGTGGTATCGCGGGCGCGATGTTTACCCGCAAGCCGCGCCAGTGGCGGATGATGTATTGGTTa < 1:1476015/228‑1 (MQ=255) gCATTGAAGCCCTGATCGGTAAAGTCCGATTTTCTCGGTTGGGGATTAAACTTGCCGAGACGCACAACGAAGGTTATCGCTGGCAGCATGAAGCGGTTATTGCTCTTGCCTCACCCGATAATATGAATGCATTCGAGCTGACACCGCAGGAAGCGGAGGAGTGGTATCGCGGGCGCGATGTTTACCCGCAAGCCGCGCCAGTGGCGGGAGGTGGATTGGGTACttttc > 1:66998/1‑226 (MQ=255) gCATTGAAGCCCTGATCGGTAAAGTCCGATTTTCTCGGTTGGGGATTAAACTTGCCGAGACGCACAACAAAGGTTATCGCTGGCAGCATGAAGCGGTTATTGCTCTTGCCTCACCCGATAATATGAATGCATTCGAGCTGACACCGCAGGAAGCGGAGGAGTGGTATCGCGGGCGCGATGTTTACCCGCAAGCCGCGCCAGTGGCGGATGATGTATTGGTTACTTTcc < 2:688922/228‑1 (MQ=255) ttGAAGCCCTGATCGGTAAAGTCCGATTTTCTCGGTTGGGGATTAAACTTGCCGAGACGCACAACAAAGGTTATCGCTGGCAGCATGAAGCGGTTATTGCTCTTGCCTCACCCGATAATATGAATGCATTCGAGCTGACACCGCAGGAAGCGGAGGAGTGGTATCGCGGGCGCGATGTTTACCCGCAAGCCGCGCCAGTGGCGGATGAGGGTTTGGTTACTTTTCA‑Gc > 1:1621308/1‑228 (MQ=255) gTAAAGTCCGATTTGCTCGGTTGGGGATTAAACTTGCCGAGACGCACAACAAAGGTTATCGCTGGCAGCATGAAGCGGTTATTGCTCTTGCCTCACCCGATAATATGAATGCATTCGAGCTGACACCGCAGGAAGCGGAGGAGTGGTATCGCGGGCGCGATGTTTACCCGCAAGCCGCGCCAGTGGCGGATGATGTATTGGTTACTTTCCA‑GCATCAACCGATTGGtt < 1:1907563/228‑1 (MQ=255) aaaGTCCGATTTTCTCGGTTGGGGATTAAACTTGCCGAGACGCACAACAAAGGTTATCGCTGGCAGCATGAAGCGGTTATTGCTCTTGCCTCACCCGATAATATGAATGCATTCGAGCTGACACCGCAGGAAGCGGAGGAGTGGTATCGCGGGCGCGATGTTTACCCGCCAGCCGCGCCAGTGGCGGATGCTGTATTGGGGACTTTTCA‑GCATCACCCGACTGGTGTa > 1:1111526/1‑228 (MQ=255) atgccGATTTTCTCGGTTGGGGATTAAACTTGCCGAGACCCACAACAAAGGTTATCGCTGGCAGCATGAAGCGGTTATTGCTCTTGCCTCACCCGATAATATGAATGCATTCGAGCTGACACCGCAGGAAGCGGAGGAGTGGTATCGCGGGCGCGATGTTTA‑CCGCAAGCCGCGCCAGTGGCGGATGATGTATTGGTTACTTTCCA‑GCATCAACCGATTGGTTTAGcc < 2:1242548/225‑1 (MQ=255) tCCGATTTTCTCGGTTGGGGATTAAACTTGCCGAGACGCACAACAAAGGTTATCGCTGGCAGCATGAAGCGGTTATTGCTCTTGCCTCACCCGATAATATGAATGCATTCGAGCTGACACCGCAGGAAGCGGAGGAGTGGTATCGCGGGCGCGATGTTTACCCGCAAGCCGCGCCAGTGGCGGGTGGTGTATTGGTTACTTTCTC‑GCGTCAACCGCTTGGtgtttccc > 2:696077/1‑221 (MQ=255) tCCGATTTTCTCGGTTGGGGATTAAACTTGCCGAGACGCACAACAAAGGTTATCGCTGGCAGCATGAAGCGGTTATTGCTCTTGCCTCACCCGATAATATGAATGCATTCGAGCTGACACCGCAGGAAGCGGAGGAGTGGTATCGCGGGCGCGATGTTTACCCGCAAGCCGCGCCAGTGGCGGATGATGTATTGGtt > 1:1239680/1‑197 (MQ=255) ccGATTTTCTCGGTTGGGGATTAAACTTGCCGAGACGCACAACAAAGGTTATCGCTGGCAGCATGAAGCGGTTATTGCTCTTGCCTCACCCGATAATATGAATGCATTCGAGCTGACACCGCAGGAAGCGGAGGAGTGGTATCGCGGGCGCGATGTTTACCCGCAAGCCGCGCCAGTGGCGGATGATGTATTGGTTa < 2:1237466/197‑1 (MQ=255) aTTTTCTCGGTTGGGGATTAAACTTGCCGAGACGCACAACAAAGGTTATCGCTGGCAGCATGAAGCGGTTATTGCTCTTGCCTCACCCGATAATATGAATGCATTCGAGCTGACACCGCAGGAAGCGGAGGAGTGGTATCGCGGGCGCGATGTTTACCCGCAAGCCGCGCCAGTGGCGGATGATGt > 1:1650274/1‑186 (MQ=255) ttttCTCGGTTGGGGATTAAACTTGCCGAGACGCACAACAAAGGTTATCGCTGGCAGCATGAAGCGGTTATTGCTCTTGCCTCACCCGATAATATGAATGCATTCGAGCTGACACCGCAGGAAGCGGAGGAGTGGTATCGCGGGCGCGATGTTTACCCGCAAGCCGCGCCAGTGGCGGATGATGTa < 2:1647355/186‑1 (MQ=255) tcGGTTGGGGATTAAACTTGCCGAGACGCACAACAAAGGTTATCGCTGGCAGCATGAAGCGGTTATTGCTCTTGCCTCACCCGATAATATGAATGCATTCGAGCTGACACCGCAGGAAGCGGAGGAGTGGTATCGCGGGCGCGATGTTTACCCGCAAGCCGCGCCAGTGGCGGATGATGGATTGGGTTCTTTTCAGGCTTCAACCACTCGGTTTTCCCaacagg > 2:1189816/1‑220 (MQ=255) cGGTTGGGGATTAAACTTGCCGAGACGCACAACAAAGGTTATCGCTGGCAGCATGAAGCGGTTATTGCTCTTGCCTCACCCGATAATATGAATGCATTCGAGCTGACACCGCAGGAAGCGGAGGAGTGGTATCGCGGGCGCGATGTTTACCCGCAAGCCGCGCCAGTGGCGGATGATGTATTGGTTACTTTCCA‑GCATCAACCGATTGGTTTAGCCAAACGGa < 1:1191950/223‑1 (MQ=255) tttGGGGATTAAACTTGCCGAGCCGCACAACAAAGGTTATCGCTGGCAGCATGAAGCGGTTATTGCTCTGGCCTCACCCGATAATATGAATGCATTCGAGCTGACACCGCAGGAAGCGGAGGAGTGGTATCGCGGGCGCGATGTTTACCCGCAAGCCGCGCCAGTGGCGGATGATGTATTGGTTACTTTCCA‑GCATCAACCGATTGGTTTAGCCAAACGGATTGGtt < 2:1113095/226‑1 (MQ=255) tGGGGATTAAACTTGCCGAACCGCACAACAAAGGTTAACGCTGGCAGCATGAAGCGGTTATTGCTCTTGCCTCACCCGATAATATGAATGCATTCGAGCTGACACCGCAGGAAGCGGAGGAGTGGTATCGCGGGCGCGATGTTTACCCGCAAGCCGCGCCAGTGGCGGATGATGTATTGGTTACTTTCCA‑GCATCAACCGATTGGTTTAGCCAAACGGATTGGTTcgc < 1:697246/228‑1 (MQ=255) gtatttatcTTGCCGATACGCACAACAAAGGTGATCGCTGGCAGCATGAAGCGGTTATTGCTCTTGCCTCACCCGATAAGATGAATGCATTCGAGCTGACACCGCAGGAAGCGGAGGAGTGGTATCGCGGGCGGGATGTTTACCCGCAAGCCGCGCCAGTGGCGGATGATGTATTGGTTACTTTCCA‑GCATCAACCGATTGGTTTAGCCAAACGGATTGGTTCGC‑GAt < 1:1947936/220‑1 (MQ=255) aTTAAACTTGCCGAGACGCACAACAAAGGTTATCGCTGGCAGCATGAAGCGGTTATTGCTCTTGCCTCACCCGATAATATGAATGCATTCGAGCTGACACCGCAGGAAGCGGAGGAGTGGTATCGCGGGCGCGATGTTTACCCGCAAGCCGCGCCAGTGGCGGATGATGTATTGGTTACTTTCCA‑GCATCAACCGATTGGTTTAGCCAAACGGATTGGTTCGC‑GATTg < 1:861837/228‑1 (MQ=255) tAAACTTGCCGAGACGCACAACAAAGGTTATCGCTGGCAGCATGAAGCGGTTATTGCTCTTGCCTCACCCGATAATATGAATGCATTCGAGCTGACACCGCAGGAAGCGGAGGAGTGGTATCGCGGGCGCGATGTTTACCCGCAAGCCGCGCCAGTGGCGGATGATGGATTGGTTACTTTCCA‑GCAGCAACCGATTGGGTTAGGCCAACGGATTGGTTCGC‑GAttta > 1:1385662/1‑225 (MQ=255) aaaCTTGCCGAGACGCACAACAAAGGTTATCGCTGGCAGCATGAAGCGGTTATTGCTCTTGCCTCACCCGATAATATGAATGCATTCGAGCTGACACCGCAGGAAGCGGAGGAGTGGTATCGCGGGCGCGATGTTTACCCGCAAGCCGCGCCAGTGGCGGATGATGTATTGGTTACTTTCCA‑GCATCAACCGATTGGTTTAGCCAAACGGATTGGTTCGC‑GATTGaaa < 1:768082/228‑1 (MQ=255) ccGAGACGCACAACAAAGGTTATCGCTGGCAGCATGAAGCGGTTATTGCTCTTGCCTCACCCGATAATATGAATGCATTCGAGCTGACACCGCAGGAAGCGGAGGAGTGGTATCGCGGGCGCGATGTTTCCCCGCAAGCCGCGCCAGTGGCGGATGATGTATTGGTTACTTTCCA‑GCATCAACCGATTGGTTTa < 1:760424/194‑1 (MQ=255) cGAGACGCACAACAAAGGTTATCGCTGGCAGCATGAAGCGGTTATTGCTCTTGCCTCACCCGATAATATGAATGCATTCGAGCTGACACCGCAGGAAGCGGAGGAGTGGTATCGCGGGCGCGATGTTTACCCGCAAGCCGCGCCAGTGGCGGAGGATGGATTGGTTACTTTTCA‑GCATCAACCGATTGGTTTTGCCAAAAGGAGTGGTTTGCGGATTTaaaaaaacca > 2:288689/1‑223 (MQ=255) gagaCGCACAACAAAGGTTATCGCTGGCAGCATGAAGCGGTTATTGCTCTTGCCTCACCCGATAATATGAATGCATTCGAGCTGACACCGCAGGAAGCGGAGGAGTGGTATCGCGGGCGCGATGTTTACCCGCAAGCCGCGCCAGTGGCGGATGATGTATTGGTTACTTTCCA‑GCATCAACCGATTGGTTTAGCCAAACGGATTGGTTCGC‑GATTGAAAAACAGCTa < 2:1650525/227‑1 (MQ=255) gagaCGAACAACAAAGGTTATCGCTGGCAGCATGAAGCGGTTATTGCTCTTGCCTCACCCGATAATATGAATGCATTCGAGCTGACACCGCAGGAAGCGGAGGAGTGGTATCGCGGGCGCGATGTTTACCCGCAAGCCGCGCCAGTGGCGGATGATGTATTGGTTACTTTCCA‑GCATCAACCGATTGGTTTAGCCAAACGGATTGGTTCGC‑GATTGAAAAACAGCTAt < 1:1584716/228‑1 (MQ=255) tgagcgcaacaaAGGTTATCGCTGGCAGAATGAAGCGGTTAGTGCTCTTGCCTCACCCGATAATATGAATGCATTCGAGCTGACACCGCAGGAAGCGGAGGAGTGGTATCGCGGGCGCGATGTTTACCCGCAAGCCGCGCCAGTGGCGGATGATGTATTGGTTACTTTCCA‑GCATCAACCGATTGGTTTAGCCAAACGGATTGGTTCGC‑GATTGAAAAACAGCTATcc < 1:1339421/222‑1 (MQ=255) acaaAGGTTATCGCTGGCAGCATGAAGCGGTTATTGCTCTTGCCTCACCCGATAATATGAATGCATTCGAGCTGACACCGCAGGAAGCGGAGGAGTGGTATCGCGGGCGCGATGTTTACCCGCAAGCCGCGCCAGTGGCGGATGATGTATTGGTTACTTTCCA‑GCATCAACCGATTGGTTTAGCCAAACGGATTGGTTCGC‑GATTGAAAAACAGCTATCCGCGTG‑aa < 2:269013/227‑1 (MQ=255) acaaAGGTTATCGCTGGCAGCATGAAGCGGTTATTGCTCTTGCCTCACCCGATAATATGAATGCATTCGAGCTGACACCGCAGGAAGCGGAGGAGTGGTATCGCGGGCGCGATGTTTACCCGCAAGCCGCGCCAGTGGCGGATGATGGATTGGTTACTTTCCA‑GCATCAACCGATTGGTTTAGCCAAACGGATTGGTTCGC‑GATTGAAAAACAGCTATCCGCGTG‑aa < 1:1832772/227‑1 (MQ=255) cGCTGGCAGCATGAAGCGGTTATTGCTCTTGCCTCACCCGATAATATGAATGCATTCGAGCTGACACCGCAGGAAGCGGAGGAGTGGTATCGCGGGCGCGATGTTTACCCGCAAGCCGCGCCAGTGGCGGATGATGGATTGGTTACTTTTCC‑GCATCCACCGAATGGGTTAGCCAAACGGGTTGGGTCGC‑GATTGCAAAACAGCTATTCCCGGG‑AACT‑GGTGCGCGAt > 1:1308519/1‑228 (MQ=255) cagcaTGAAGCGGTTATTGCTCTTGCCTCACCCGATAATATGAATGCATTCGAGCTGACACCGCAGGAAGCGGAGGAGTGGTATCGCGGGCGCGATGTTTACCCGCAAGCCGCGCCAGTGGCGGAGTGTGGATTGGTTAATTTTCa > 2:1955387/1‑146 (MQ=255) agcaTGAAGCGGTTATTGCTCTTGCCTCACCCGATAATATGAATGCATTCGAGCTGACACCGCAGGAAGCGGAGGAGTGGTATCGCGGGCGCGATGTTTACCCGCAAGCCGCGCCAGTGGCGGATGATGTATTGGTTACTTTCCA‑g < 1:1958875/146‑1 (MQ=255) tGCCTCACCCGATAATATGAATGCATTCGAGCTGACACCGCAGGAAGCGGAGGAGTGGTATCGCGGGCGCGATGTTTACCCGCAAGCCGCGCCAGTGGCGGATTGTGGATTTGTTTATTTCCC‑GCATCAACCCCTTGGTTTTGGCAAACCGACTGGGTTGC‑GATTGAAAAACAGCCATCCGCGCT‑CACT‑GGGGGGCCGT‑GGGGAACTTCTTTCCGTGTACGCCTG‑GC‑a > 2:31596/1‑228 (MQ=255) tGCCTCACCCGATAATATGAATGCATTCGAGCTGACACCGCAGGAAGCGGAGGAGTGGTATCGCGGGCGCGATGTTTACCCGCAAGCCGCGCCAGTGGCGGATGATGTGTTGGGTTCTTTCCA‑GCATCAACCGATTGGTTTTGCCCAAAGGATTTGGTTGC‑GCTTGTAAAACAGCTATCCGCGTG‑AACT‑GGGGCGCGCT‑GGGGAACCTGTTTACGGTCAAGCCTG‑Act > 2:1589550/1‑227 (MQ=255) tGCCTCACCCGATAATATGAATGCATTCGAGCTGACACCGCAGGAAGCGGAGGAGTGGTATCGCGGGCGCGATGTTTACCCGCAAGCCGCGCCAGTGGCGGATGATGTATTGGTTTCTTTCCC‑CCATCAACCCATTGGGTTAGCCAAAAGGATTGGGTCGC‑GGTTTAAAAAAAGCAATCCCCGCGAAACTGGGTGGGCG‑T‑GGGAACATTTTTCCCGTTAA‑GCCTG‑GC‑a > 2:950779/1‑228 (MQ=255) gCCTCAACCGATAATATGAATGCATTCGAGCTGACACCGCAGGAAGCGGAGGAGTGGTATCGCGGGCGCGATGTTTACCCGCAAGCCGCGCCAGTGGCGGATGATGTATTGGTTACTTTCCA‑GCATCAACCGATTGGTTTAGCCAAACGGATTGGTTCGC‑GATTGAAAAACAGCTATCCGCGTG‑AACT‑GGTGCGCGAT‑GGGAAACTTTTTACCGGTa < 1:952466/217‑1 (MQ=255) cacACCCGATAATATGAATGCATTCGAGCGGCCACCGCAGGAAGCGGAGGAGTGGTATCGCGGGAGCGATGTTTACCCGCAAGCCGCGCCAGTGGAGGATGATGTATTGGTTACTTTCCA‑GCATCAACCGATTGGTTTAGCCAAACGGATTGGTTCGC‑GATTGAAAAACAGCTATCCGCGTG‑AACT‑GGTGCGCGAT‑GGGAAACTTTTTACCGGTAACGCCTG‑AC‑Agcg < 1:1762830/226‑1 (MQ=255) ataataTGAATGCATTCGAGCTGACACCGCAGGAAGCGGAGGAGTGGTATCGCGGGCGCGATGTTTACCCGCAAGCCGCGCCAGTGGCGGATGATGGATTGGTTACTTTCCA‑GCATAAACCGATTGGGTTAGACAAACGGATTGGTTTGC‑GATTGAAAAACAGCTATCCGCGGG‑AAAT‑GGGGGGCGATGGGGAAAATTTTTAcg > 2:1765688/1‑203 (MQ=255) taataTTAATGCATTCGAGCTGACGCCGCAGGAAGCGGAGGAGTGGTATCGCGGGCGCGATGTTTACCCGCAAGCCGCGCCAGTGGCGGATGATGTATTGGTTACTTTCCA‑GCATCAACCGATTGGTTTAGCCAAACGGATTGGTTCGC‑GATTGAAAAACAGCTATCCGCGTG‑AACT‑GGTGCGCGAT‑GGGAAACTTTTTACCgg < 1:1768812/204‑1 (MQ=255) taataTGAATGCATTCGAGCTGACACCGCAGGAAGCGGAGGAGTGGTATCGCGGGCGCGATGTTTACCCGCAAGCCGCGCCAGTGGCGGATGATGTATTGGTTACTTTCCA‑g > 2:1424050/1‑112 (MQ=255) aataTGAATGCATTCGAGCTGACACCGCAGGAAGCGGAGGAGTGGTATCGCGGGCGCGATGTTTACCCGCAAGCCGCGCCAGTGGCGGATGATGTATTGGTTACTTTCCA‑Gc < 1:1426606/112‑1 (MQ=255) gAATGCATTCGAGCTGACACCGCAGGAAGCGGAGGAGTGGTATCGCGGGCGCGATGTTTACCCGCAAGCCGCGCCAGTGGCGGATGATGTATTgg > 2:1502330/1‑95 (MQ=255) gAATGCATTCGAGCTGACACCGCAGGAAGCGGAGGAGTGGTATCGCGGGCGCGATGTTTACCCGCAAGCCGCGCCAGTGGCGGATGATGGATTGGTTACTTTTCA‑GCATCAACCGATTGGGTTATGCAAACGGATTGGTTCTC‑GATTGAAAAACAGCTa > 2:1944735/1‑159 (MQ=255) aaTGCATTCGAGCTGACACCGCAGGAAGCGGAGGAGTGGTATCGCGGGCGCGATGTTTACCCGCAAGCCGCGCCAGTGGCGGATGATGTATTGGt < 1:1505015/95‑1 (MQ=255) aaTGCATTCGAGCTGACACCGCAGGAAGCGGAGGAGTGGTATCGCGGGCGCGATGTTTACCCGCAAGCCGCGCCAGTGGCGGATGATGTATTGGTTACTTTCCA‑GCATCAACCGATTGGTTTAGCCAAACGGATTGGTTCGC‑GATTGAAAAACAGCTAt < 1:1948193/159‑1 (MQ=255) aTGCATTCGAGCTGACACCGCAGGAAGCGGAGGAGTGGTATCGCGGGCGCGATGTTTACCCGCAAGCCGCGCCAGTGGCGGCTGATGGATTGGGTACTTTTCA‑GCATCAACCCATTGGTTTAGCCCAACGGATTGGTTCGC‑GATTGGAAAAAAACCATCCCCGGG‑AACT‑GGGTGGCCGT‑GGCGAACTTTTTACCGGTTATGCCTG‑GCAAGCG‑ACCAAAAAAgcggca > 2:2010955/1‑224 (MQ=255) ggCTGACCCCGAAGGAAGCGGAGGAGTGGTATCGCGGGCGCGATGTTTACCCGCAAGCCGCGCCAGTGGCGGATGATGTATTGGTTACTTTCCA‑GCATCAACCGATTGGTTTAGCCAAACGGATTGGTTCGC‑GATTGAAAAACAGCTATCCGCGTG‑AACT‑GGTGCGCGAT‑GGGAAACTTTTTACCGGTAACGCCTG‑AC‑AGCGCACAAAAAAAGCGCACTTTTTGACTg < 2:1618445/227‑1 (MQ=255) acacCGCAGGAAGCGGAGGAGTGGTATCGCGGGCGCGATGTTTACCCGCAAGCCGCGCCAGTGGCGGATGATGTATTGGTTACTTTCCA‑GCATCAACCCATTGGGTTTGCCCAAAGGGTTGGTTTGC‑GGTTGGAAAACAAGCATCCCCGTT‑ACCC‑GGGGGGCGGG‑TGGAAACCTTTTTCCGGGAAAGCCCGAAC‑AG‑GCCCAAAAAAAACGCAACTTTTgtattggac > 2:17102/1‑219 (MQ=255) cacCGGAGGAAGCGGAGGAGTGGTATCGCGGGCGCGATGTTTACCCGCAAGCCGCGCCAGTGGCGGATGATGTATTGGTTACTTTCCA‑GCATCAACCGATTGGTTTAGCCAAACGGATTGGTTCGC‑GATTGAAAAACAGCTATCCGCGTG‑AACT‑GGTGCGCGAT‑GGGAAACTTTTTACCGGTAACGCCTG‑AC‑AGCGCACAAAAAAAGCGCACTTTTTGACTGGcaca < 1:675343/227‑1 (MQ=255) ggaGGAAGCGGGGGAGTGGTATCGGGGGCGCGATTTTTACTCGGAAGCCGCGCCAGGGGCGGATGATGTATTGGTTACTTTCCA‑GCATCAACCGATTGGTTTAGCCAAACGGATTGGTTCGC‑GATTGAAAAACAGCCATCCGCGTG‑AACT‑GGTGCGCGAT‑GGGAAACTTTTTACCGGTAACGCCTG‑AC‑AGCGCACAAAAAAAGCGCACTTTTTGACTGGCACATTCgg < 2:1383177/226‑1 (MQ=255) ggaGGAAGCGGAGGATTGGTATCGCGGGCGCGATGTTTACCCGCAAGCCGCGCCAGTGGCGGATGATGTATTGGTTACTTTCCA‑GCATCAACCGATTGGTTTAGCCAAACGGATTGGTTCGC‑GATTGAAAAACAGCTATCCGCGTG‑AACT‑GGTGCGCGAT‑GGGAAACTTTTTACCGGTAACGCCTG‑AC‑AGCGCACAAAAAAAGCGCACTTTTTGACTGGCACATTCgg < 1:289182/226‑1 (MQ=255) gaaGGAAGCGTAGGGGTGGTATCGCGGGCGCGATGTTTCCCCGCAAGCCGCGCCAGTGGCGGATGATGTATTGGTTACTTTCCA‑GCATCAACCGATTGGTTTAGCCAAACGGATTGGTTCGC‑GATTGAAAAACAGCTATCCGCGTG‑AACT‑GGTGCGCGAT‑GGGAAACTTTTTACCGGTAACGCCTG‑AC‑AGCGCACAAAAAAAGCGCACTTTTTGACTGGCACATTCgg < 2:652655/226‑1 (MQ=255) gCAGGGAGGGGAGGAGTGGTAGCGCGGGCGCGATGTTTACCCGCAAGCCGCGCCAGTGGCGGATGATGTATTGGTTACTTTCCA‑GCATCAACCGATTGGTTTAGCCAAACGGATTGGTTCGC‑GATTGAAAAACAGCTATCCGCGTG‑AACT‑GGTGCGCGAT‑GGGAAACTTTTTACCGGTAACGCCTG‑AC‑AGCGCACAAAAAAAGCGCACTTTTTGACTGGCACATTCgg < 1:617024/228‑1 (MQ=255) cAGGAAGCGGAGGAGTGGTATCGCGGGCGCGATGTTTACCCGCAAGCCGCGCCAGTGGCGGATGATGTATTGGTTACTTTCCA‑GCATCAACCGATTGGTTTAGCCAAACGGATTGGTTCGC‑GATTGAAAAACAGCTATCCGCGTG‑AACT‑GGTGGGCGAT‑GGGGAACTTTTTTCCCGTAACGCCCG‑AC‑AACGCCCCAAAAAAGGGGACCTTTTGACTTGCACCATCGGc > 2:1644947/1‑228 (MQ=255) aaGCGGAGGAGTGGTATCGCGGGCGCGATGTTTACCCGCAAGCCGCGCCAGTGGCGGATGATGTATTGGTTACTTTCCA‑GCATCAACCGATTGGTTTAGCCAAACGGATTGGTTCGC‑GATTGAAAAACAGCTATCCGCGTG‑AACT‑GGTGCGCGAT‑GGGAAACTTTTTACCGGTAACGCCTG‑AC‑AGCGCACAAAAAAAGCGCACTTTTTGACTGGCACATTCGGCTGc < 1:17136/227‑1 (MQ=255) ggaggaGTGGTATCGCGGGCGCGATGTTTACCCGCAAGCCGCGCCAGTGGCGGATGATGTATTGGTTACTTTCCA‑GCATCAACCGATTgg > 2:1689379/1‑90 (MQ=255) gaggagTGGTATCGCGGGCGCGATGTTTACCCGCAAGCCGCGCCAGTGGCGGATGATGTATTGGTTACTTTCCA‑GCATCAACCGATTGGt < 1:1692368/90‑1 (MQ=255) tGGTATCGCGGGCGCGATGTTTACCCGCAAGCCGCGCCAGTGGCGGATGATGTATTGGTTACTTTCCA‑GCATCAACCGATTGGTTTAGCCAAACGGATTGGTTCGC‑GATTGAAAAACAGCTATCCGCGTG‑AACT‑GGTGCGCGAT‑GGGAAACTTTTTACCGGTAACGCCTG‑AC‑AGCGCACAAAAAAAGCGCACTTTTTGACTGGCACATTCGGCTGCCTCAACTAgg < 2:1113776/226‑1 (MQ=255) gTATCGCGGGCGCGATGTTTACCCGCAAGCCGCGCCAGTGGCGGATGATGTATTGGTTACTTTCCA‑GCATCAACCGATTGGTTTAGCCAAACGGATTGGTTCGC‑GATTGAAAAACAGCTATCcgc < 1:1081677/125‑1 (MQ=255) cgGTCGCGATGTTTACCCGCAAGCCGCGCCAGTGGCGGATGATGTATTGGTTACTTTCCA‑GCATCAACCGATTGGTTTAGCCAAACGGATTGGTTCGC‑GATTGAAAAACAGCTATCCGCGTG‑AACT‑GGTGCGCGAT‑GGGAAACTTTTTACCGGTAACGCCTG‑AC‑AGCGCACAAAAAAAGCGCACTTTTTGACTGGCACATTCGGCTGCCTCAACTAGGCTGAAAAAt < 1:1277451/227‑1 (MQ=255) cgGGCGCGATGTTTACCCGCAAGCCGCGCCAGTGGCGGATGATGGATTGGTTAATTTTCC‑GCATCAACCGATTGGTTTAGCCCAAAGGATTGGTTTGC‑GATTTAAAAACAGGTATTCGCGTG‑AAAT‑GGGGGGGGGT‑GGGGAAATTTTTAACggg > 2:1051050/1‑153 (MQ=255) gggCGCGATGTTTACCCGCAAGCCGCGACAGTGGCGGATGATGTATTGGTTACTTTCCA‑GCATCAACCGATTGGTTTAGCCAAACGGATTGGTTCGC‑GATTGAAAAACAGCTATCCGCGTG‑AACT‑GGTGCGCGAT‑GGGAAACTTTTTACCGGTa < 1:1052943/154‑1 (MQ=255) ggCGCGATGTTTACCCGCAAGCCGCGCCAGTGGCGGATGATGGATTGGTTACTTTCCA‑GCATCAACCGATTGGTTTAGCCAAAAGGGTTGGTTCGC‑GATTGAAAAACAGCTATCCGCGTG‑AACT‑GNNNNNCGAT‑GGGAAACTTTTTACCGGTAACGCCTG‑AC‑Agc > 2:1836424/1‑165 (MQ=255) ggCGCGATGTTTACCCGCAAGCCGCGCCAGTGGCGGAAGGTGTAATGGGTTCTTTCCC‑GCATCAACCGATTGGGTTAGCCAAAAGGGTTGGTTCGC‑GATTGAAAAACAGATATCCGCGGG‑GACT‑GGGGCGGGAT‑GGGGAAATTTTTTCCGGTAACGGCTG‑GC‑AGCGCACCAAAAAAACGCACCTTTTTACTGGCACATTCGTCTGCCTCAACTaaggcggcaaat > 2:701508/1‑214 (MQ=255) tcagggtGTTTACCCGCAAGCCGCGCCAGTGGCGGATGATGTATTGGTTACTTTCCA‑GCATCAACCGATTGGTTTAGCCAAACGGATTGGTTCGC‑GATTGAAAAACAGCTATCCGCGTG‑AACT‑GGTGCGCGAT‑GGGAAACTTTNNNCCNGTAACGCCTG‑AC‑Agcg < 1:1839683/159‑1 (MQ=255) cgcgATGTTTACCCGCAAGCCGCGCCAGTGGCGGATGATGTATTGGTTACTTTTCA‑GCAACAACCGAATGGTTTAGGCCAACGGATTGGTTCGC‑GATTGAAAAACAGCCATCCGCGGG‑AAAT‑GGGGCGGGAG‑GGGAAACGTTTTACCGGTAAAGCCTG‑AC‑AACGCACAAAAAAAGAGCCCtt > 2:419598/1‑182 (MQ=255) gcgATGTTTACCCGCAAGCCGCGCCAGTGGCGGATGATGTATTGGTTACTTTCCA‑GCATCAACCGATTGGTTTAGCCAAACGGATTGGTTCGC‑GATTGAAAAACAGCTATCCGCGTG‑AACT‑GGTGCGCGAT‑GGGAAACTTTTTACCGGTAACGCCTG‑AC‑AGCGCACAAAAAAAGCGCACttt < 1:420300/182‑1 (MQ=255) gcgATGTTTACCCGCAAGCCGCGCCAGTGGCGGATGATGTATTGGTTACTTTCCA‑GCATCAACCGATTGGTTTAGCCAAACGGATTGGTTCGC‑GATTGAAAAACAGCTATCCGCGTG‑AACT‑GGTGCGCGAT‑GGGAAACTTTTTACCGGTAACGCCTG‑AC‑AGCGCACAAAAAAAGCGCACTTTTTGACTGGAACATTCgg < 1:1843378/199‑1 (MQ=255) ttGTTTACCCGAAGGCCGGGACGGGTGCGGAGTATTTATTTGTTACTTTCCC‑GCACTAACGTATTGGTTTAGCCAAACGGATTGGTTCGC‑GATTGAAAAACAGCTATCCGCGTG‑AACT‑GGTGCGCGAT‑GGGAAACTTTTTACCGGTAACGCCTG‑AC‑AGCGCACAAAAAAAGCGCACTTTTTGACTGGCACATTCGGCTGCCTCAACTAGGCTGAAAAATGGTGCGAt < 2:1570463/226‑1 (MQ=255) ttACCCGCAAGCCGTGCCAGTGGCGGATGATGTATTGGTTACTTTCCA‑GCATCAACCGATTGGTTTAGCCAAACGGATTGGTTCGC‑GATTGAAAAACAGCTATCCGCGTG‑AACT‑GGTGCGCGAT‑GGGAAACTTTTTACCGGTAACGCCTG‑AC‑AGCGCACAAAAAAAGCGCACTTTTTGACTGGCACATTCGGCTGCCTCAACTAGGCTGAAAAATGGTGCGATCGGAc < 2:1557658/228‑1 (MQ=255) ttACCCGCAAGCCGCGCCAGTGGCGGATGATGTATTGGTTACTTTCCA‑GCATCAACCGATTGGTTTAGCCAAACGGATTGGTTCGC‑GATTGAAAAACAGCTATCCGCGTG‑AACT‑GGTGCGCGAT‑GGGAAACTTTTTACCGGTAACGCCTG‑AC‑AGCGCACAAAAAAAGCGCACTTTTTGACTGGCACATTCGGCTGCCTCAACTAGGCTGAAAAATGGTGCGATCGGa < 1:1301697/227‑1 (MQ=255) aagcccGCAAGCCGCGCCAGTGGCGGATGATGTATTGGTTACTTTCCA‑GCATCAACCGATTGGTTTAGCCAAACGGATTGGTTCGC‑GATTGAAAACCAGCTATCCGCGTG‑AACT‑GGTGCGCGAT‑GGGAAACTTTTTACCGGTAACGCCTG‑AC‑AGCGCACAAAAAAAGCGCACTTTTTGACTGGCACATTCGGCTGCCTCAACTAGGCTGAAAAATGGTGCGATCGGAc < 1:1733452/225‑1 (MQ=255) cAAGCCGCGCCAGTGGCGGATGATGTATTGGTTAATTTCCA‑GCATCAACCGATTGGTTTAGCCAAACGGGTTGGTTTGC‑GATTGAAAAAAAGCTATCCGCGTG‑AACT‑GGTGCGCGGT‑GGGAAACTTTTTTCCg > 2:1212123/1‑133 (MQ=255) aaGGCGCGCCAGTGGTGGGTGATGTATTGGTTACTTGCCA‑GCATCAACCGATTGGTTTAGCCAAACGGATTGGTTCGC‑GATTGAAAAACAGCTATCCGCGTG‑AACT‑GGTGCGGGAT‑GGGAAACTTTTGACCGGTAACGCCTG‑AC‑AGCGCACAAAAAAAGCGCACTTTTTGACTGGCACATTCGGCTGCCTCAACTAGGCTGAAAAATGGTGCGATCGGACTgg < 1:834045/223‑1 (MQ=255) aaGCCGCGCCAGTGGCGGATGATGTATTGGTTACTTTCCA‑GCATCAACCGATTGGTTTAGCCAAACGGATTGGTTCGC‑GATTGAAAAACAGCTATCCGCGTG‑AACT‑GGTGCGCGAT‑GGGAAACTTTTTACCgg < 1:1214291/133‑1 (MQ=255) ccGCGCCAGTGGCGGATTATGTATTGGTTACTTTCCA‑GCAACAACCGATTGGTTTAGCCAAACGGATTGGTTCGC‑GATTGAAAAACAGCTATCCGCGTG‑AACT‑GGTGCGCGAT‑GGGAAACTTTTTACCGGTAACGCCTG‑AC‑AGCGCACAAAAAAAGCGCACTTTTTGACTGGCACATTCGGCTGCCTCAACTAGGCTGAAAAATGGTGCGATCGGACTGGTCGTACca < 2:1597586/228‑1 (MQ=255) cgcCAGTGGCGGATGATGTATTGGGTACTTTTCA‑GCATCAACCGATTGGGTTAGCCCAACGGATTTGGTCGC‑GATTGAAAAACAGCTATCCGCGTG‑AACT‑GGGGCGCGGT‑GGGAAAAttttt > 1:218794/1‑122 (MQ=255) cgcCAGTGGCGGATGATGGATTGGTTACTTTCCA‑GCATCAACCCATTGGTTTAGCCAAAAGGATTGGTTCGC‑GGTTGAAAAAAAGCTATCCGCGTG‑AACC‑GGGGCGCGAT‑GGGGAACTTTTTACCGGTAACGCCTG‑AC‑AGCGCACAAAAAAAACGCACTTTTTGACTGGCACATTCGGCTGCCTCAAATAGGGTGAAAAATGGTGTGGTCGGGCCGGTCggaacccaa > 2:571401/1‑220 (MQ=255) gcCAGTGGCGGATGATGTATTGGTTACTTTCCA‑GCATCAACCGATTGGTTTAGCCAAACGGATTGGTTCGC‑GATTGAAAAACAGCTATCCGCGTG‑AACT‑GGTGCGCGAT‑GGGAAACTTTTTa < 2:218421/122‑1 (MQ=255) aGTGGCAGATGATGGATTGGTTACTTTCCA‑GCATCAACCGATTGGTTTAGCCAAACGGATTGGTTCGC‑GATTGAAAAACAGCTATCCGCGTG‑AACT‑GGTGCGCGAT‑GGGAAACTTTTTACCGGTAACGCCTG‑AC‑AGCGCACAAAAAAAGCGCACTTTTTGACTGGCACATTCGGCTGCCTCAACTAGGCTGAAAAATGGTGCGATCGGACTGGTCGTACCACAATCgg < 1:1658797/228‑1 (MQ=255) aGTGGAGGATGACGTATTGGTTACTTACCA‑GCATCAACCTGTTGGTGTAGCCAAACGGATTGGGTCGC‑GATTGAAAAACAGCTATCCGCGTG‑AACT‑GGTGCGCGAT‑GGGAAACTTTTTACCGGTAACGCCTG‑AC‑AGCGCACAAAAAAAGCGCACTTTTTGACTGGCACATTCGGCTGCCTCAACTAGGCTGAAAAATGGTGCGATCGGACTGGTCGTACCACAATCgg < 2:1476444/228‑1 (MQ=255) gTGGGGGATGATGTATGGTTTACTTTCCA‑GCATCAACCGATTGGTTTAGCCAAACGGATTGGTTCGC‑GATTGAAAAACAGCTATCCGCGTG‑AACT‑GGTGCGCGAT‑GGGAAACTTTTTACCGGTAACGCCTG‑AC‑AGCGCACAAAAAAAGCGCACTTTTTGACTGGCACATTCGGCTGCCTCAACTAGGCTGAAAAATGGTGCGATCGGACTGGTCGTACCACAATCgg < 1:1647861/227‑1 (MQ=255) tgatgTATTGGTTACTTTCCA‑GCATCAACCGATTGGTTTAGCCAAACGGATTGGTTCGC‑GATTGAAAAACAGCTATCCGCGTG‑AACT‑GGTGCGCGAT‑GGGAAACTTTTTAACGGTAACGCCTG‑AC‑AGCGCACAAAAAAAGCGCACTTTTTGACTGGCACATTCGGCTGCCTCAACTAGGCTGAAAAATGGTGCGATCGGACTGGTCGTACCACAATCGGCAGCTAAAt > 1:1313988/1‑228 (MQ=255) tgTATTGGTTACTTTCCA‑GCATCAACCGATTGGTTTAGCCAAACGGATTGGTTCGC‑GATTGAAAAACAGCTATCCGCGTG‑AACT‑GGTGCGCGAT‑GGGAAACTTTTTACCGGTAACGCCTG‑AC‑AGCGCACAAAAAAAGCGCACTTTTTGACTGGCACATTCGGCTGCCTCAACTAGCCTGAATACTGGTGTGATCGGACTGGTCGTACCCCAATCGGCAGCTAAATGga > 2:1987560/1‑228 (MQ=255) | GTTCCCGGTGGGCATTGAAGCCCTGATCGGTAAAGTCCGATTTTCTCGGTTGGGGATTAAACTTGCCGAGACGCACAACAAAGGTTATCGCTGGCAGCATGAAGCGGTTATTGCTCTTGCCTCACCCGATAATATGAATGCATTCGAGCTGACACCGCAGGAAGCGGAGGAGTGGTATCGCGGGCGCGATGTTTACCCGCAAGCCGCGCCAGTGGCGGATGATGTATTGGTTACTTTCCA‑GCATCAACCGATTGGTTTAGCCAAACGGATTGGTTCGC‑GATTGAAAAACAGCTATCCGCGTG‑AACT‑GGTGCGCGAT‑GGGAAACTTTTTACCGGTAACGCCTG‑AC‑AGCGCACAAAAAAAGCGCACTTTTTGACTGGCACATTCGGCTGCCTCAACTAGGCTGAAAAATGGTGCGATCGGACTGGTCGTACCACAATCGGCAGCTAAATGGA > NC_000913/1921320‑1921769 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |