Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,206,438	G→A	14.4%	intergenic (‑182/‑25)	ttdR ← / → ttdA	transcriptional activator of ttdABT/L‑tartrate dehydratase, alpha subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,206,438	0	G	A	14.4%	116.7 / 3.3	55	intergenic (‑182/‑25)	ttdR/ttdA	transcriptional activator of ttdABT/L‑tartrate dehydratase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base G (8/38);  new base A (6/2);  total (15/40)
Fisher's exact test for biased strand distribution p-value = 2.39e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TG‑AA‑TTTTT‑CCAGACAAATACAAAAACTGGA‑GTTGC‑CATGAT‑GAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGAC  >  NC_000913/3206215‑3206657                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                cAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TG‑AA‑TTTTT‑CCAGACa                                                                                                                                                                                                                              <  2:114381/227‑1 (MQ=255)    tACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTTG‑AA‑TTTTT‑CTAGAAAAAt                                                                                                                                                                                                                           >  1:187119/1‑228 (MQ=255)           aaGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TG‑AA‑TTTTT‑CCAGACAAATACAAAAAc                                                                                                                                                                                                                   <  2:824723/228‑1 (MQ=255)           aaGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TG‑AA‑TTTTT‑CCAGACAAATACAAAAAc                                                                                                                                                                                                                   <  1:1116341/228‑1 (MQ=255)                tttGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TG‑AA‑ATTTT‑TTAGACAAATACaaaaaaccgag                                                                                                                                                                                                              >  1:548686/1‑222 (MQ=255)                     cTAAAGGCAAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TG‑AA‑TTTTT‑CCAGACAAATACAAAAACTGGA‑GTTGc‑c                                                                                                                                                                                                       <  2:1693330/228‑1 (MQ=255)                              aGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑GG‑AACTTTTT‑CAAGCCAAA‑ACAAAAAATAGAGGTTGC‑CA‑GAT‑GAgg                                                                                                                                                                                             >  2:1287567/1‑227 (MQ=255)                                 tATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TG‑AA‑TTTTT‑CCAGACAAATACAAAAACTGGA‑GTTGC‑CATGAT‑GAGCGa                                                                                                                                                                                           <  2:1526099/227‑1 (MQ=255)                                     cAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TG‑GA‑ATTTT‑TCAGACCAAAACAAAAACATGA‑GTTGT‑Catgat‑gtggtgaaaaa                                                                                                                                                                                      >  1:463733/1‑218 (MQ=255)                                            gTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TG‑AA‑TTTTT‑CCAGACAAATACAAAAACTGGA‑GTTGC‑CATGAT‑GAGCGAAAGtaat                                                                                                                                                                                    <  2:449352/223‑1 (MQ=255)                                             ctAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑GG‑AA‑ATTTT‑TCAGACAAAAACAAAAAAATAA‑GTTGC‑CCTGAT‑TAGAGGAAAtaaataataa                                                                                                                                                                              >  1:1347672/2‑221 (MQ=255)                                               aaTTAACCGCAGTTGTCATAAGCGAAGTGTATTTCGCAAAACATTGCCATTCCCTGCGCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TG‑AA‑TTTTT‑CCAGACAAATACAAAAACTGGA‑GTTGC‑CATGAT‑GAGCGAAAGTAATAAGCAACa                                                                                                                                                                            <  2:56735/228‑1 (MQ=255)                                                       gCAGTTGTGATAAGCGTAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TG‑AA‑TTTTT‑CCAGACAAATACAAAAACTGGA‑GTTGC‑CATGAT‑GAGCGAAAGTAATAAGCAACAGGCa                                                                                                                                                                        <  1:1493377/224‑1 (MQ=255)                                                         aGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TG‑AA‑TTTTT‑CCAGACAAATACAAAAACTGGA‑GTTGC‑CATGAT‑GAGCGAAAGTAATAAGCAACAGGCAGTGAAt                                                                                                                                                                  <  1:1613654/228‑1 (MQ=255)                                                               tatgaGCGCAGTGTATTTCACAAAACATTGCCATTCCCTGCTCTGGAGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TG‑AA‑TTTTT‑CCAGACAAATACAAAAACTGGA‑GTTGC‑CATGAT‑GAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTg                                                                                                                                                            <  2:1180064/224‑1 (MQ=255)                                                                      gcAGTTTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TG‑AA‑TTTTT‑CCAGACAAATACAAAAACTGGA‑GTTGC‑CATGAT‑GAGCGAAAGTAATAAGCAACAgg                                                                                                                                                                          <  1:671658/207‑1 (MQ=255)                                                                      gcAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TG‑AA‑TTTTT‑CCAGACAAATACAAAAACTGGA‑GTTGC‑CATGAT‑GAGCGAAAGta                                                                                                                                                                                      <  1:541637/195‑1 (MQ=255)                                                                          tgtATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TG‑AA‑TTTTT‑TCAAACAAATACAAAAAATGAG‑GTTGC‑CATGAT‑GAGCGAAAGTAATAAGAAACAGGACGGGAAAAAAATGAAAGAGATTg                                                                                                                                                  >  1:1103403/1‑227 (MQ=255)                                                                          tgtATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TG‑AA‑ATTTT‑CCAAACAAAAAAAAAAAATGTG‑TTTTC‑CATGTT‑GAGTGGAAGTAAAAATAAACAGGCAATGGAAAAACTTACAAAGATTg                                                                                                                                                  >  1:1392280/1‑227 (MQ=255)                                                                           gtATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TG‑AA‑TTTTT‑CCAGACAAATACAAAAACTGGA‑GTTGC‑CATGAT‑GAGCGAAAGTAATAAGCAACAGGCAGTGAATAAg                                                                                                                                                               <  1:1107714/213‑1 (MQ=255)                                                                                 cGCAAAACATTGCCATTCCTTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TG‑AA‑TTTTT‑CCAGACAAATACAAAAACTGGA‑GTTGC‑CATGAT‑GAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTa                                                                                                                                            <  1:406921/226‑1 (MQ=255)                                                                                           tGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TG‑AA‑TTTTT‑TCAGAc                                                                                                                                                                                                                               >  2:430697/1‑136 (MQ=255)                                                                                            gCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TG‑AA‑TTTTT‑CCAGACa                                                                                                                                                                                                                              <  1:431414/136‑1 (MQ=255)                                                                                               atccccTGCTCTGCCAAATTTTTCATTCTTATTTCGGCTTTAATTCCGAAACTGTTAATTAAATATTCCCGGTGGAAAAAAATAAAAACCCCAATTTTTAGTGGTAGTTTGTGGCGG‑TG‑AA‑TTTTT‑CCAGACAAATACAAAAACGGGA‑GTTGC‑AATGAT‑GAGCAAAAGTAAAAAGCAACAGGCAGTAAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATG‑At                                                                                                                           <  2:547785/225‑1 (MQ=255)                                                                                                           ggcaaaaTATTCATTCTTATTTTCGCTTTAATTCACGAACTTTAAATTCCTAATTCCGGGGGGAAAAAAAAAAAAAACCAAATTTTTAGGGGTGGTTGGTGGGGG‑TA‑AA‑TTTTT‑CCAGACAAATACAAAAACTGGA‑GTTGC‑CATGAT‑GAGCGAAAGTAATAAGCAACGGGCAGTGAATAAGTGGACAGAGATTGTCGCTAATTTTACCGCCATG‑ATTTCTACCCGAAt                                                                                                               <  2:1345255/223‑1 (MQ=255)                                                                                                                  tatTCATTCTTATTTAGGGGTTGATTCACAAACTGTTAATTACATATTCACGGTGGAAAAAAATAAAAAACCACATTTTTAGTGGTAGTTTGGGGCGG‑TG‑AA‑TTTTC‑CCAGACAAATACAAAAACTGGA‑GTTGC‑AATGAT‑GAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGTTTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGcc                                                                                                            <  2:186796/224‑1 (MQ=255)                                                                                                                         tcTTATTTACGCGTAGATTCACGAACTGTAAATTACATATTCACGGTGGAAAAAAATATAAAACCACATTTTTAGTGGTATTTTGTGGCGG‑TA‑AA‑TTTTT‑CAAGACAAATACAAAAACGGGA‑TTTGC‑AATGAT‑GAGCGAAAGTAAAAAGCAACAGGCAGTAAATAAGTTGACAGAGTTTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACgtg                                                                                                  <  2:462974/227‑1 (MQ=255)                                                                                                                          cTTATTTTCACGTTGATTCACGAACTGTTAATTACATATTCACGTTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TG‑AA‑TTTTT‑CCAGACAAATACAAAAACTGGA‑GTTGC‑CATGAT‑GAGCGAAAGta                                                                                                                                                                                      <  1:972679/143‑1 (MQ=255)                                                                                                                             aTTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TG‑AA‑TTTTT‑TCAGAAAAAAACAAAAAATGGA‑GGTGT‑CATGAT‑GAACGGAAGTAATAAACAAAAAGAAGTGAATAAATTGACAGAGAATTTGGCCAACATTAACTCCCTG‑AATTTTATCCCAAAACCTGATGAAGtggtggg                                                                                             >  2:1642189/1‑227 (MQ=255)                                                                                                                               tgtCGCGTTGATCCACGAACTGTTAATTACATATTCTCGGCGACAAAAAATATAAACCCACATTTTTAGTGGTAGTTTGTGGCGG‑TG‑AA‑TTTTC‑CCAGACAAATACAAAAACTGGA‑GTTGC‑CATGAT‑GAGCAAAAGTAATAAGCAACAGGCAGTGAATAAGTTGAAAGAGATTGTCGCTACCTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATa                                                                                           <  2:1389784/226‑1 (MQ=255)                                                                                                                                     gTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑GGAAA‑TTTTT‑TCA‑ACAAAAAAAAAAAAAGGG‑GTTGG‑CAAGGTGGAGGGAAAGAAAAAAAAAAAAGCAAGGAAAAAAGTT‑AAAGAGAATGTTGGTAAATTTTACGCCAAGAATTTTAACCCGAATGCC‑GATGACGaggtggggaaaatca                                                                                     >  2:132518/1‑219 (MQ=255)                                                                                                                                     gTTGATTCACGAACTGTTAATTACATATTCACGGTGGAAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TG‑AG‑TTTTT‑CCAGACAAATAAAAAAACTGGA‑GTGGC‑CATGAT‑GAGCGAAAGTAATAAGCAACAGGAAGTAAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaa                                                                                     <  1:485324/228‑1 (MQ=255)                                                                                                                                        gATTCACGAACTGTTAATTTAATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTGGTGGCGG‑TG‑AA‑TTTTT‑CCAGACAAATACAAAAACTGGA‑GTTGC‑CATGAT‑GAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCg                                                                                                                                               <  1:1970367/168‑1 (MQ=255)                                                                                                                                        aaTTCACGACTTGTTAATTACATATTCCCGGTAGAAAAAAATAAAAACCCACTTTTTTAGTGGTATTTTGTGGCGT‑TG‑AA‑TTTTTCCCAGACAAATAAAAAAACTGGA‑GTTGC‑CATGAT‑GAGCGAAAGTAATAAGCAACAGGCAGTAAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaaa                                                                                   <  2:1571295/227‑1 (MQ=255)                                                                                                                                                   tGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TG‑GA‑ATTTT‑TCAAACAAAAACAAAAAATGGA‑GTTGG‑CCTGGT‑GAGGGGAAGTAATAAACAACAAGCAGAGAATAAAATGACAGAGATTGTCGCTAAATTTAACGCCACG‑CATTCTACCCCAAAACCCGATGACGTGGGGGAGAAAATAAAAAAACTAAAAGaa                                                                       >  2:553778/1‑227 (MQ=255)                                                                                                                                                    gTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TG‑AA‑ATTTT‑TCAAACAAAAACAAAAAATGGA‑GTTGG‑CATGAT‑GAGCGAAAGTAAAAAACaaaaag                                                                                                                                                                          >  2:1614769/1‑125 (MQ=255)                                                                                                                                                     ttAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TG‑AA‑TTTTT‑CCAGACAAATACAAAAACTGGA‑GTTGC‑CATGAT‑GAGCGAAAGTAATAAGCAACAGGc                                                                                                                                                                         <  1:1617627/129‑1 (MQ=255)                                                                                                                                                               atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TG‑AATTTTTT‑CCAaaaaaaaaaaaaaaagga                                                                                                                                                                                                                >  2:1441328/1‑80 (MQ=255)                                                                                                                                                               atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑GG‑AA‑TTTTT‑TCAAAAAAAAAAAAAAAATGGA‑GGTTC‑CCTTAT‑GAGGGAAAGTAAAAAGAAAAAAGAAGTGAATAAATTGACaaaagag                                                                                                                                                   >  2:1796910/1‑135 (MQ=255)                                                                                                                                                                ttCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TG‑AA‑TTTTT‑CCAGACAAATACAAAAACTGGA‑g                                                                                                                                                                                                             <  1:1443909/84‑1 (MQ=255)                                                                                                                                                                ttCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TG‑AA‑TTTTT‑CCAGACAAATACAAAAACTGGA‑GTTGC‑CATGAT‑GAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTg                                                                                                                                                  <  1:1800093/141‑1 (MQ=255)                                                                                                                                                                       tGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑GG‑AA‑TTTTT‑TCAAACAAAAAAAAAAAAAGGA‑GGTGG‑CCTGAT‑GAGCGAAAGTAATAAAAAACAAGCAGAGAAAAAGTTGACAGAGAATGTTGCTAACATTATCGCCCTG‑ATTTTTACCCGAATGCCTGATGACGGGGTGGGTAAAATAAAACAACTTAAAGATGCCGTAAAGTACTCGATggg                                                   >  2:1747471/1‑228 (MQ=255)                                                                                                                                                                        ggCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TG‑AA‑TTTTT‑CCAGACAAATACAAAAACTGGA‑GTTGC‑CATGAT‑GAGCGAAAGtaat                                                                                                                                                                                    <  1:52004/99‑1 (MQ=255)                                                                                                                                                                        ggCAAAAAATATAAAACCACATTTGTAGTGGTAGTTTGTGGCGG‑TG‑AA‑TTTTT‑CCAGACAAATACAAAAACTGGA‑GTTGC‑CATGAT‑GAGCGAAAGTAATAAGCAACAgg                                                                                                                                                                          <  1:1886026/109‑1 (MQ=255)                                                                                                                                                                                atatAAACCCAAATTTTTAGTGTTAGTTTGGGGCGG‑TG‑AA‑TTTTC‑CCAGACAAATAAAAAAACTGGA‑GTTGC‑CATGAT‑GAGCGAAAGTAATAAGCAACGGGCAGTGAATAAGTTGACAGAGATTGTCCCTACCTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTAt                                          <  1:1750562/228‑1 (MQ=255)                                                                                                                                                                                atatAAAACCAATTTTTTGGTGGTAGTTTGTGACGG‑TA‑AA‑TTTTA‑CCAGACAAATACAAAAACTGGA‑GTTGC‑CATGAT‑GAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGAAAGAGATGGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTAt                                          <  1:1645100/228‑1 (MQ=255)                                                                                                                                                                                        ccaatttttgAGTGGAAGTTTGGGGCGG‑TG‑AA‑TTTTC‑CCAAACAAATACAAAAACTGGA‑GTTGC‑CATGAG‑GAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAATTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATa                                  <  1:1158144/223‑1 (MQ=255)                                                                                                                                                                                        ccACATTTTTAGTGGTAGTTTGTGGCGG‑TA‑AT‑TTTTC‑CCAGAAAAATACAAAAACTGGA‑TTTGC‑CATGAT‑GAGCAAAAGAAAAAAGCAACGGGCAGTGAATAAGTGGACAGAGTTTGTCGATAATTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGTATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATa                                  <  1:182942/228‑1 (MQ=255)                                                                                                                                                                                          aaaTTTTTAGTGGAAGTTTGGGGCGG‑TA‑AA‑TTTTT‑ACAGAAAAAAAAAAAAAGTGGA‑GTTGC‑CATGAT‑GAGCAAAAGTAATAAGCAACGGGCGGTGAATAAGTTGACAGAGATTGTCGTTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACg                                <  2:1920289/226‑1 (MQ=255)                                                                                                                                                                                                     ggTAGTTTGGGGCGG‑TA‑AA‑TTTTT‑CCAGAAAAATACAAAACCGGGA‑GTTGC‑AATGAT‑GAGCGAAAGTAATAAGCAACAGGCAGTAAATAAGTTGACAGAGATTGTCGCTAATTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGacaa                     <  1:1289873/228‑1 (MQ=255)                                                                                                                                                                                                         gTTTGTGGGGG‑TG‑AA‑TTTTT‑CAAGAAAAATACAAAAACTGGA‑GTGGC‑CATGAT‑GAGAGAAAGTAATAAGCAACGGGCAGTAAATAAGTTGACAGAGATTGTCGCTACCTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACgtg                                                                                                  <  1:556600/147‑1 (MQ=255)                                                                                                                                                                                                             ggggCGG‑TA‑AA‑TTTTT‑CCAAAAAAATACAAAAACTGGA‑GTTGC‑CATGAT‑GAGCGAAAGTAAAAAGCAACAGGCAGTAAATAAGTTGACAGAGATTGTCGATAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCaaa             <  1:1054330/226‑1 (MQ=255)                                                                                                                                                                                                                   g‑TG‑AA‑TTTTA‑CAAGACAAATACAAAAACTGGA‑GTTGC‑CATGAG‑AGGCAAAAGTAATAAGAAACAGCAAGTAAATAAGTTGACAGAGTTTTGCGCTAATTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGa       <  1:7361/228‑1 (MQ=255)                                                                                                                                                                                                                     tG‑AA‑TTTTA‑CAAGACAAATAAAAAAAGGGGA‑TTTGC‑AATGAT‑GAGCGAAAGAAATAAACAACAGGCAGGAAAAAAGTTGAAAGAGTTTGTCGATAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGa       <  1:1251443/227‑1 (MQ=255)                                                                                                                                                                                                                           ttttc‑CAAGAAAAATACAAAACCGGGA‑‑TTGCACTTATT‑GACAGAAAATAATAAGCAACAGGAAGTAAAAAAGTGAACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGAc  <  2:358707/228‑1 (MQ=255)                                                                                                                                                                                                                                  cAGACAAATACAAAAACTGGA‑GTTGC‑CATGAT‑GAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTg    >  1:1321428/1‑220 (MQ=255)                                                                                                                                                                                                                                    gACAAATACAAAAACTGGA‑GTTGC‑CATGAT‑GAGCGAAAGTAATAAGCAACAGGCAGTGa                                                                                                                                                                    >  1:418176/1‑59 (MQ=255)                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                CAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TG‑AA‑TTTTT‑CCAGACAAATACAAAAACTGGA‑GTTGC‑CATGAT‑GAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGAC  >  NC_000913/3206215‑3206657

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑