| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 766,716 | 0 | G | T | 23.9% | 143.6 / 20.4 | 67 | A245S (GCG→TCG) | mngA | fused 2‑O‑a‑mannosyl‑D‑glycerate specific PTS enzymes: IIA component/IIB component/IIC component |
| Reads supporting (aligned to +/- strand): ref base G (28/23); new base T (16/0); total (44/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.48e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TGGATGACAAGGGAGGCACGCAACCTTCTGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGG‑ATCAACTCAATAGTG‑CA‑A‑CCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGC‑GTTTTAATGGCATTCCCGCGCTT‑TCAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCA‑ACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTA‑CCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCG > NC_000913/766486‑766952 | tGGATGACAAGGGAGGCACGCAACCTTCTGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGG‑ATAAACACCAACTGG‑CA‑A‑CCGCCTGTGTTTTTGGGGCTGaa > 1:1687661/1‑239 (MQ=255) gAGGCACGCAACCTTCTGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGG‑GTCAACTCAATAGTG‑CA‑A‑CCGCCCGGTTTTTTTCGGCTGGAGGCGCCATCaaa > 1:2656592/1‑238 (MQ=255) gAGGCACGCAACCTTCTGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGG‑ATCCAATCAAAAATG‑CC‑A‑ACCCCTGTATTTTTTCGGCTGAAGTCGCCCTCaaa > 1:447644/1‑238 (MQ=255) cGCAACCTTCTGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGCCGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGG‑AACAACTCAAAAAGG‑CA‑A‑CCGCCTCTATTTTTTCGGCTGGAATGTCCCTCAAGGGGAGt > 1:218992/1‑239 (MQ=255) ttttCCAACGCGCCAACTATCGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGG‑ATCAACTCAATAGGG‑CA‑A‑CCGCCCGTATTTTTTCGGCTGGAGTCGCCATCAAGGAGAGTGAGC‑GGTTTAATTGCAt > 1:347523/1‑239 (MQ=255) gcCAACTATCGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGG‑ATCAACTCAATAGTG‑CA‑A‑CCGCCTGTATTTTTTCGGGTGAAGGTGCCATCAAGGGGAGGGAGC‑GGTTTAATGGGCTTccccgccct > 1:1136907/1‑232 (MQ=255) cTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGG‑ATCAACTCAATAGTG‑CA‑A‑CCGCCTGTATTTTTGCGGCTGAAGGCGCCATCAAGGGGAGGGAGC‑GGTTTAATg > 1:1890002/1‑183 (MQ=255) cTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGG‑ATAAACTCAATAGTG‑GA‑A‑CCGCCTGTTTTTTTTCGGCTGAAGTCGCCATCAAGGAGAGGGGGG‑GGTTTTAAGGCATTCCCGCGCTT‑TTAGTGc > 1:2432293/1‑204 (MQ=255) acCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGG‑ATCAACTCAATAGTG‑CA‑A‑CCGCCCGTATTTTTGCGGCTGGAGTCGCCCTCAAGGGGAGTGAGC‑GTTTTAATGGCAATCCCGCGCTT‑TCAGttc > 1:1131820/1‑197 (MQ=255) tatGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGG‑ATCAACTCAATATTG‑CA‑C‑CCGCCTGTATTTTTGCGGCTGGAGGCCCCATCAAGGAGAGTGAGC‑GTTTTAATGTGATTCCCGCGGTT‑TCGTTGCCTGTTGCCGAGCCGCTTTGCCATTCAGAAGCGTTgcgtccacaa > 2:729463/1‑229 (MQ=255) tatGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGG‑ATCAACTCAATAGTG‑CA‑A‑CCGCCTGTATTTTTTCGGCTGAAGTCGCCATCAAGGAGAGTGAGC‑GTTTTAATGGCATTCCCGCGCTT‑TCCGTGCCTGTTGCCGAGCCGATTCGCCCTGCAGAAGCGTTTATCCA‑ACAAg > 1:2384463/1‑239 (MQ=255) cTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGG‑GTAAAATAAAAAGAG‑CA‑A‑CCGCCTGGATTTTTTCGGCTGAGGTCGCACTCAAGGAGAAAGAGG‑GGTTTTCTGTCACTCCCGCGCTTCTTGGTGCCGCTTG‑CGAACCGATTCCTCATGCGGAAGAGATGAGcctaaagatcgtt > 2:1031038/1‑228 (MQ=255) cTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGG‑ATCAACTCAATAGTG‑CA‑A‑CCGCCTGTATTTTTTCGGCTGAAGTCGCCATCAAGGAGAGTGAGC‑GTTTTAATGGCATTCCCGCGCTT‑TCAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCA‑ACAAGCGCtt > 1:2225907/1‑239 (MQ=255) cTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGG‑ATCAACTCAATAGGT‑CA‑A‑ACGCCTGTATTTTTTGGGCTGAAGGAGCCATCAAGGGGAGTGGGC‑GTTTTAATTGGATTTCCCCGGTT‑TCAGTGCCCGGTGGCGAGCCGCGTGACCCATCAGAAGCCTTGATTCT‑CCAAgcggct > 2:798044/1‑236 (MQ=255) cTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGG‑AACAACTCAATAATG‑GA‑A‑CCCCCTGTATTTTTTTGGCTGAAGTCGCCCCCAAGGAGAGGGGGC‑GGTTTTATGGCATTTCCCCGCTT‑TTCGTGGCTGTTGCCGCGCCGGGTTGCCCTGCCGAGGCGGTGTTCCC‑ACAAAGGCtt > 2:136104/1‑239 (MQ=255) gCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGG‑ATCAACCCAATAAGG‑CA‑A‑CCGCCTGTATTTTTTCGGCTGAAGTCGCCATCaaa > 1:2391570/1‑146 (MQ=255) aaGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGG‑ATCAACTCAAAAATG‑CA‑A‑CCGCCTGTAATTTTGCGGCCGAAGGCGCCATCaaaggga > 1:1964114/1‑130 (MQ=255) gCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGG‑ATCAACTCAATAGTG‑CA‑A‑CCGCCTGTATTTTTGTGGCGGAAGCCGCCACCAAGGAGAGTGAGCGGTTTTTATGGC‑TTCCCGCGGTT‑TCAGCTCCCGTTGCCGCGCCCGTTCTCCCTGCAGAAGAGTCTATCTACACAA‑CGTCTACCCCCAAGCGTACCGTTgt > 2:1489260/1‑238 (MQ=255) ccGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGG‑ATCAACTCAATAGTG‑CA‑A‑CCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGC‑GTTTTAATGGCATTCCCGCGCTT‑TCAGTGCCTGTTGCCGAGCCGGTTCGCCATGCAGAAGCGTTGATCCA‑ACAAGCGCTTACCCTCAAGCGTAGCGATgag > 1:1137133/1‑239 (MQ=255) gACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGG‑ATCAAATCAATAGTG‑CA‑A‑CCGCCTGTATTTTTTCGGGTGGAGTGGCCATCAAGGAGAGGGAGC‑GGTTTTATGGGCTTTCCGCGCTT‑TTAGTGCCTGTTGTCCAGGCGGATTGGCATGCCGCAGCGTTGATCCC‑AACAGCGCTTATACCCAAACGTcgcgctacggt > 2:818125/1‑231 (MQ=255) cGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGG‑ATCAACTCAATAGTG‑CA‑A‑CCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGGGAGC‑GTTTTAATGGCATTCCCGCGCTT‑TCAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCA‑ACAAGCGCTTAc > 1:1872053/1‑204 (MQ=255) cGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGG‑ATCAACTCAATAGTG‑CA‑A‑CCGCCTGGATTTTTTCGGCTGAAGTCGCCATCAAGGGGAGGGAGC‑GTTTTAATGGCATTCCCGCGCTT‑TCAGTGc > 1:387068/1‑152 (MQ=255) gcgtgaaaaaaaaaGGC‑CAAACGGGTATGGAGGGGGTTTAAAGGGGG‑GACAAACCAAAAGGGCCA‑A‑CCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGC‑GTTTTAATGGCATTCCCGCGCTT‑TCAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCA‑ACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTA‑CCGTACAGCAAGATACGCaa < 1:10985/233‑1 (MQ=255) aaaaaCAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGG‑ATCAACTCAATAGTG‑CA‑A‑CCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGGGAGC‑GTTTTAATGGCATTCCCGCGCTT‑TCAGTGCCTGttt > 1:361507/1‑140 (MQ=255) aaCAAGGCGC‑AACGGGCGTGAAGGGGTTTTAACGGGGGAATCACCTAAAAAGTG‑AA‑A‑CCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGC‑GTTTTAATGGCATTCCCGCGCTT‑TCAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCA‑ACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTA‑CCGTACAGCAAGATACGCAACCGGTGaaa < 1:136104/239‑1 (MQ=255) ggCGCTAACGGCATTGAAGGGCGTTTAACGGCGG‑ATCAACCCAATAGTG‑CC‑A‑CCCCCCGGATTTTTTCGGCTGAAGTCGCCATCAAGGGGAGGGAGC‑GTTTTTATGGGATTTCCGCCCTT‑TTAGTGCCTGTTtcc > 2:30028/1‑135 (MQ=255) ccgcaaggggCATGGAGGGGCTTTTAAGGGGGA‑ATCAACTAAATGGGG‑CA‑A‑CCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGC‑GTTTTAATGGCATTCCCGCGCTT‑TCAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCA‑ACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTA‑CCGTACAGCAAGATACGCAACCGGTGaaa < 1:1940586/225‑1 (MQ=255) cgcTAACGGCATTGAAGGGCGTTTAACGGCGG‑ATAAAATAAATAAAA‑CA‑A‑ACGCCCGTATTTTTTGGGCTGGAGACGACATCAAGGGGGGTGAGG‑GGTTTAATGGGCTTCCCGCGCTT‑TTAGCGCCAGTTGCCCAGGCGGTTATACCCACCGGAGAGTTGCTCCA‑ACCAGAGCTGTCCCCCAAGCGTAGGGGGGCGACGCGTACCCGAACA‑AAAGATACGCCACCGGTGGAAAGAGTcc > 2:1123450/1‑238 (MQ=255) cgcAAACGGAATGGAGGGGTGTTAAACGGGGG‑AACACCTAAATGGTG‑AA‑T‑CCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGC‑GTTTTAATGGCATTCCCGCGCTT‑TCAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCA‑ACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTA‑CCGTACAGCAAGATACGCAACCGGTGAAAAgtgt < 1:2395910/237‑1 (MQ=255) ccatAACGGCATAGAGGGGTGTTAAGCGGCGA‑ATAAACAAAATGGGG‑CA‑A‑CCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGC‑GTTTTAATGGCATTCCcg < 1:1409330/110‑1 (MQ=255) aaCGGAATGGAGGGGCTTTTAGCGGCGA‑ATAAACAAAATGGTG‑CA‑A‑CCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAg < 2:41940/81‑1 (MQ=255) acggagtagaggggCGTTTAAGGGAGG‑AACAAC‑CAAAAGGT‑CACC‑CCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGGGC‑GTTTTAATGGCATTCCCGCGCTT‑TCAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCA‑ACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTA‑CCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAAc < 1:2070188/227‑1 (MQ=255) aTTGAAGGGCGTTTAACGGCGG‑ATAAACTCAAAAGTG‑CA‑A‑CCCCCTGTATTTTTGCGGCTGAAGACGCCATCCAGGAAGGGGGGG‑GTTTTTATGGCATTTCCCCGCtt‑ttcga > 2:1409330/1‑109 (MQ=255) aaGGGCGTTTAACGGCGG‑ATCAACTCAATAGTG‑CA‑A‑CCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGc > 1:41940/1‑81 (MQ=255) ggCGTTTAACGGCGG‑ATCAACTCAATAGTG‑CA‑A‑CCGCCTGTATTTTTGCGGCTGAAGGCGCCATCAAGGAGAGGGAGC‑GGTTTAATGGCATTCCCGCGCTT‑TCAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGGTGATCCA‑ACAAGCGCTTAcc > 1:369594/1‑161 (MQ=255) tttAACGGCGG‑ATCAACTCAATAGTG‑CA‑A‑CCCCCTGTATTTTTGCGGCTGAAGTCGCCCTCCAGGAGAGTGAGC‑GTTTTAATGGCATTCCCGCGCCT‑TCCGTGCCTGTTGGCGAGCCGGTTCGCCCTTCAGAAGCGCTTTTCCA‑ACAAGCGCTTCCCCTCCAGCGGAGCGATGAGAACCGTA‑CCGTACAGCAAGATACGCAACCGGGGAAAAGGAGCAAAACGGAGCTGAAAAAGGCa > 2:1241222/1‑238 (MQ=255) gttAACCGGGG‑AACAACCAAAAAGTG‑GA‑ACCCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGC‑GTTTTAATGGCATTCCCGCGCTT‑TCAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCA‑ACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTA‑CCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCa < 1:1272414/238‑1 (MQ=255) aaaagccAACTAAATGGCG‑CA‑G‑CCGCCTGTATTTTTGCGGCTGAAGTCGCAATCAAGGAGAGTGAGC‑GTTTTAATGGCATTCCCGCGCTT‑TCAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCA‑ACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTA‑CCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGTAGCTGAAACAGGCACTGTTGAg < 2:1989491/233‑1 (MQ=255) cAATAGTG‑CA‑A‑CCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGC‑GTTTTAATGGCATTCCCGCGCTT‑TCAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCA‑ACAAGCGCTTACCCTCAAGCGTAGCGATGAGAc > 2:398421/1‑159 (MQ=255) cAATAGTG‑CA‑A‑CCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGC‑GTTTTAATGGCATTCCCGCGCTT‑TCAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCA‑ACAAGCGCTTACCCTCAAGCGTAGCGATGAGAc < 1:398421/159‑1 (MQ=255) tAGTG‑CA‑A‑CCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGC‑GTTTTAATGGCATTCCCGCGCTT‑TCAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCA‑ACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTA‑CCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATctct > 1:1989491/1‑235 (MQ=255) tAGTG‑AA‑A‑CCGCCTGTATTTTTGCGGCTGAAGTCGCCAt < 1:1306570/39‑1 (MQ=255) tG‑CA‑A‑CCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGC‑GTTTTAATGGCATTCCCGCGCTT‑TCAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCA‑ACAAGCGCTTACCCTc > 1:2627400/1‑136 (MQ=255) tG‑CA‑A‑CCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGC‑GTTTTAATGGCATTCCCGCGCTT‑TCAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCA‑ACAAGCGCTTACCCTc < 2:2627400/136‑1 (MQ=255) gcaacccGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGC‑GTTTTAATGGCATTCCCGCGCTT‑TCAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCA‑ACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTA‑CCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGcc < 1:729463/233‑1 (MQ=255) cA‑A‑CCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGC‑GTTTTAATGGCATTCCCGCGCTT‑TCAGTGCCTGTTGCCGAGCCGATTCGcc < 1:44368/99‑1 (MQ=255) cA‑A‑CCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGC‑GTTTTAATGGCATTCCCGCGCTT‑TCAGTGCCTGTTGCCGAGCCGATTCGcc > 2:44368/1‑99 (MQ=255) cA‑A‑CCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGC‑GTTTTAATGGCATTCCCGCGCTT‑TCAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCA‑ACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTA‑CCGTACAGCAAGATACGCaa > 1:1136643/1‑176 (MQ=255) cA‑A‑CCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGC‑GTTTTAATGGCATTCCCGCGCTT‑TCAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCA‑ACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTA‑CCGTACAGCAAGATACGCaa < 2:1136643/176‑1 (MQ=255) cA‑A‑CCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGC‑GTTTTAATGGCATTCCCGCGCTT‑TCAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCA‑ACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTA‑CCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAAc > 1:2011219/1‑207 (MQ=255) cA‑A‑CCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGC‑GTTTTAATGGCATTCCCGCGCTT‑TCAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCA‑ACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTA‑CCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGt > 1:1365161/1‑238 (MQ=255) cA‑A‑CCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGC‑GTTTTAATGGCATTCCCGCGCTT‑TCAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCA‑ACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTA‑CCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGt > 1:954754/1‑238 (MQ=255) cA‑A‑CCGCCTGTATTTTTGCGGCTGAAGCCGCCATCAAGGAGAGTGAGC‑GTTTTACTGGCATTCCCGCGCTT‑TCAGAGCCTGTTGCCGAGCCGATTCGCCATGCAGACGCGTTGATCCA‑ACCACCGCTTACCCTCAACCGTAGCGCTGAGACGCTCA‑CCCTGCTGCACATTTCTCACTCAGAGAAAACTGTCGAAACGGACGTGAAACAGGAACTGTGGAGCGCACTCCCTTTTGccta > 2:2695071/1‑236 (MQ=255) aa‑a‑CCGCCTGTATTTTTGCGGATGAAGTCGCCATCAAGGAGAGTGAGC‑GTTTTAATGGCATTCCCGCGCTT‑TCAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCA‑ACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTA‑CCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTa < 2:447644/238‑1 (MQ=255) atccGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGC‑TTTTTAATGGCATTCCCGCGCTT‑TCAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCA‑ACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTA‑CCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGcc < 1:211294/233‑1 (MQ=255) ccGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGC‑GTTTTAATGGCATTCCCGCGCTT‑TCAGTGCCTGTTGCCGAGCCGATTCg < 2:2370858/94‑1 (MQ=255) ccGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGC‑GTTTTAATGGCATTCCCGCGCTT‑TCAGTGCCTGTTGCCGAGCCGATTCg > 1:2370858/1‑94 (MQ=255) ccGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGC‑GTTTTAATGGCATTCCCGCGCTT‑TCAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCA‑ACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTA‑CCGTACAGCAAGCTACTCAACCGGAGAAAAGTGTCAAAAAGGAGCTGAAACAGGCACTGTGGAGCGGAATCACTTATGCCGt > 2:797644/1‑235 (MQ=255) cGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGC‑GTTTTAATGGCATTCCCGCGCTT‑TCAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCA‑ACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTA‑CCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTa < 1:798044/235‑1 (MQ=255) gCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGt > 2:1306570/1‑39 (MQ=255) gCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGC‑GTTTTAATGGCATTCCCGCGCTT‑TCAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCA‑ACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTA‑CCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGtt > 1:1162877/1‑239 (MQ=255) cTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGC‑GTTTTAATGGCATTCCCGCGCTT‑TCAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCA‑ACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTA‑CCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTa < 1:64340/232‑1 (MQ=255) cTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGC‑GTTTTAATGGCATTCCCGCGCTT‑TCAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCA‑ACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTA‑CCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCg < 1:2440770/235‑1 (MQ=255) ttGCGGCTGAAGTCGCCATCAAGGAGAGTGAGC‑GTTTTAATGGCATTCCCGCGCTT‑TCAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCA‑ACAAgcgc < 2:1638545/111‑1 (MQ=255) ttGCGGCTGAAGTCGCCATCAAGGAGAGTGAGC‑GTTTTAATGGCATTCCCGCGCTT‑TCAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCA‑ACAAgcgc > 1:1638545/1‑111 (MQ=255) ttGCGGCTGAAGTCGCCATCAAGGAGAGTGAGC‑GTTTTAATGGCATTCCCGCGCTT‑TCAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCA‑ACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTA‑CCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTcgcg > 1:310878/1‑239 (MQ=255) ttGCGGCTGAAGTCGCCATCAAGGAGAGTGAGC‑GTTTTAATGGCATTCCCGCGCTT‑TCAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCA‑ACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTA‑CCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTcgcg < 1:1486825/239‑1 (MQ=255) | TGGATGACAAGGGAGGCACGCAACCTTCTGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGG‑ATCAACTCAATAGTG‑CA‑A‑CCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGC‑GTTTTAATGGCATTCCCGCGCTT‑TCAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCA‑ACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTA‑CCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCG > NC_000913/766486‑766952 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |