| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,151,689 | G→T | 50.3% | intergenic (+43/+22) | yegJ → / ← yegK | uncharacterized protein/ser/thr phosphatase‑related protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,151,689 | 0 | G | T | 50.3% | ‑4.7 / 3.2 | 15 | intergenic (+43/+22) | yegJ/yegK | uncharacterized protein/ser/thr phosphatase‑related protein |
| Reads supporting (aligned to +/- strand): ref base G (5/2); new base T (4/3); total (9/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.67e-01 | |||||||||||
ATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTT‑CGCTAATCAGCCGTTATATTTATTGTTTAAACTGGC‑GGTTGAA‑TTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGG‑GCTGGACAAAAACTGCTTTA‑ACAATTCAGGTAATAAATCG‑AGTTGTTCCTGCGTTGCTGCCGCCAGTCCA‑TT‑GAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGAT > NC_000913/2151578‑2151911 | aTTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTT‑CGCTAATCAGCCGTTATATTTATTGTTTAAACTGGC‑GGTTGAA‑TTGTTGTCTTCAACCCCCCTGAGAAACTCACTCTTCCCCCAGCGCCAGTGCCAACGTTTTTTCATCATCCGTACGCTCGTTTACCGCCCG‑GCTGGGCCAAAACTGCTTTT‑ACAATTCAGGTAATAAAACG‑Agt > 1:842521/1‑239 (MQ=255) gATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTT‑CGCTAATCAGCCGTTATATTTATTGTTTAAACTGGC‑GGTTGAA‑TTGTTTTTTTTAACCGCCATGAGAAACTCACTCTTCCCACAGCGCCCGTGCCAAAGTTTTTTCATTATTCGTACGCCCGGTTAACCCCCG‑GCCGGACAAAAAATTCGTTT‑ACAATTTAGGGGATAAAtacaggtgt > 2:248226/1‑231 (MQ=255) ttcttttATGGGTCACCTATCAAAAAAAAGAAGCCGACGAGAAAAGAGCAAAATTTGTT‑CGCAAACCACCTTTTATTTTTTTTTTTTAAACGGGG‑GGTTGAT‑TTTTTCTCTCCACCCGCCATGAGAAACTCACTCTGCCCACAgcgc < 2:881304/143‑1 (MQ=255) ggTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTT‑CGCTAATCAGCCGTTATATTTATTGTTTAAACTGGC‑GGTTGAA‑TTGTTTTTTTTAAACGCCCTGAGAAAATCACTCCTCCCCCCGCCCCAGTGCCAACGGTTTTTTATCATCCCTACGCTCGTTTATCGCCGGTGCGGGAGAAAAA‑TGCCTTATAAAATACAGGT‑ATAAATCG‑AGTTGTTCCTGccgtgg > 2:1209329/1‑234 (MQ=255) ggTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTT‑CGCTAATCAGCCGTTATATTTATTGTTTAAACTGGC‑GGTTGAA‑TTGTTGTCTTCAACCGCCATGAGAAAATCACTCTGCCCACAGCGCCAGGGCCAAc > 1:881304/1‑147 (MQ=255) cTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTT‑CGCTAATCAGCCGTTATATTTATTGTTTAAACTGGC‑GGTTTAA‑TTGTTTTCTTCAAACCCCATGAGAAACTCACTCTGCCCACAGCGCCCGTGCCAACg > 1:695524/1‑142 (MQ=255) cgcagaagaGCAAAGAACAATAGTTGTT‑CGATAATCCGCTGTTATATTTATTGTAAAAACGGGG‑GGTTGAA‑TTGTTTTTTAAAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGG‑GCTGGACAAAAACTGCTTTA‑ACAATTCAGGTAATAAATCG‑AGTTGTTCCTGCGTTGCTGCCGCCAGTCCA‑TT‑Gaaaaa < 1:477991/232‑1 (MQ=255) cggacgaGAAAAGAAAAAATTTAGTT‑CTCAAATCAGCTTTTATTTTTTTTG‑TTAAACCGGG‑GGTTTAATTTTTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGG‑GCTGGACAAAAACTGCTTTA‑ACAATTCAGGTAATAAATCG‑AGTTGTTCCTGCGTTGCTGCCGCCAGTCCA‑TT‑Gaaaaa < 1:64596/235‑1 (MQ=255) acgaGCTAAGAGCAAAATTAGTT‑CGCTAATCAGCCGTTATATTTATTGTTTAAACTGGC‑GGTTGAA‑ATGTTGTCTTCAACCGCCCTGAGAAAATCACTCTGCCCCCAGCGCCAGGGCCAACGGTTTTTCATCAACCGTACGCTCGGTTACCGCCCG‑GCTGGACAAAAAATGCTTTT‑ACAATTCAGGTAATAAATCG‑AGTTGTTCCTTCGTTTCTGCCGCCAGTCCACTT‑TAAAAACggg > 2:738822/1‑239 (MQ=255) cgaGCTAAGAGCAAAATTAGTT‑CGCTAATCAGCCGTTATATTTATTGTTTAAACTGGC‑GGTTGAA‑TTGTTTTTTTCAACCGCCATGAGAAAATCACCCTGCCCCCAGCGCCACTGCCAACGGTTTTTCATCATCCGTACGCCCCTTTACCGCCGG‑GCTGGGCAAAAACTGCTTTT‑ACAATTCAGGGAATAAATCGCAGTT‑TTTCTTCGTTGCTGCCGCCAGTCCA‑TTGGAAAAAAgggg > 2:64596/1‑239 (MQ=255) aaaTTTGTTCCGCTAACCAGCTTTTATTTTTATTGTTAAACGGGGC‑GGTAAAA‑TTGTGTTCTTCACCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGG‑GCTGGACAAAAACTGCTTTA‑ACAATTCAGGTAATAAATCG‑AGTTGTTCCTGCGTTGCTGCCGCCAGTCCA‑TT‑GAAAAACGGGGTAAAGAAAGGcac < 1:706623/239‑1 (MQ=255) cttttatttttatttTTAAACCGGGC‑GTTTAAT‑TTTTTGTCTTAAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGG‑GCTGGACAAAAACTGCTTTA‑ACAATTCAGGTAATAAATCG‑AGTTGTTCCTGCGTTGCTGCCGCCAGTCCA‑TT‑Gaaaaa < 1:915936/198‑1 (MQ=255) ttttAAACGGGGGGGTTGAT‑TTGTTTTCTTCACCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGG‑GCTGGACAAAAACTGCTTTA‑ACAATTCAGGTAATAAATCG‑AGTTGTTCCTGCGTTGCTGCCGCCAGTCCA‑TT‑GAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAg < 1:738822/238‑1 (MQ=255) ggC‑GGTTGAA‑TTTTTGTCTTTAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCCG‑GCTGGACAAAAACTGCTTTT‑ACAATTCAGGGAATAAATAC‑AGTTGTTCCTTCGTTGCTGCCGCCAGTCCC‑TT‑TTAAAAAGGGGGAAAGAAATGCACATTAGGGGAAATATACAACACATTTTAAGCCCGTCGCttgga > 2:641927/1‑234 (MQ=255) gC‑GGTTGAA‑TTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCCCAGCGCCAGTGCCCACGGTTTTTCATCATCCGTACGCTCGTTTACCGCCCG‑GCTGGACAAAAAATGCTTTT‑ACAATTCAGGGAATAAAACG‑AGTTGTTCCTGCGTTTCTGCCGCCAGTCCA‑TT‑GAAAAACAGGGGAAAGAAAGGCACATTAGGGGAATTATCCAGCATATTTaa > 2:394269/1‑222 (MQ=255) | ATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTT‑CGCTAATCAGCCGTTATATTTATTGTTTAAACTGGC‑GGTTGAA‑TTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGG‑GCTGGACAAAAACTGCTTTA‑ACAATTCAGGTAATAAATCG‑AGTTGTTCCTGCGTTGCTGCCGCCAGTCCA‑TT‑GAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGAT > NC_000913/2151578‑2151911 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |