Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	104,147	T→G	S56A (TCC→GCC)	ftsA →	ATP‑binding cell division FtsK recruitment protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	104,147	0	T	G	100.0%	268.1 / NA	86	S56A (TCC→GCC)	ftsA	ATP‑binding cell division FtsK recruitment protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (42/44);  total (42/44)

AGGCTGGGCGCCCTTGCCGCCAGAGGAATCTACTCAGCAACAAAATCAGGCACAGGCAGAACAACAATGATCAAGGCGACGGACAGAAAACTGGTAGTAGGACTGGAGATTGGTACCGCGAAGGTTGCCGCTTTAGTAGGGGAAGTTCTGCCCGACGGTATGGTCAATATCATTGGCGTGGGCAGCTGCCCGTCGCGTGGTATGGATAAAGGCGGGGTGAACGACCTCGAATCCG‑TGGTCAAGTGCGTACAACGCGCCATTGACCAGGCAGAATTGATGGCAGATTGTCAGATCTCTTCGGTATAT‑CTGGCGCTTTCTGGTAAGCACATCAGCTGCCAGAATGAAATTGGTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGAGCATCGTGTGCTGCATGTGATCCCGCAA  >  NC_000913/103916‑104380                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                            aGGCTGGGCGCCCTTGCCGCCAGAGGAATCTACTCAGCAACAAAATCAGGCACAGGCAGAACAACAATGATCAAGGCGACGGACAGAAAACTGGTAGTAGGACTGGAGATTGGTACCGCGAAGGTTGCCGCTTTAGTAGGGGAAGTTCTGCCCGACGGTATGGTCAATATCATTGGCGTGGGCAGCTGCCCGTCGCGTGGTATGGATAAAGGCGGGGTGAACGACCTCGAAGCCG‑Tg                                                                                                                                                                                                                                       <  1:746644/237‑1 (MQ=255)            ccTTGCCGCCAGAGGAATCTACTCAGCAACAAAATCAGGCACAGGCAGAACAACAATGATCAAGGCGACGGACAGAAAACTGGTAGTAGGACTGGAGATTGGTACCGCGAAGGTTGCCGCTTTAGTAGGGGAAGTTCTGCCCGACGGTATGGTCAATATCATTGGCGTGGGCAGGTGCCCGTCGCGTGGTATGGATAAAGGCGGGGTGTACCACACCGCAGCCGCTGGTC‑AGTGCGtca                                                                                                                                                                                                                          >  2:911836/1‑237 (MQ=255)                gccgccAGAGGAATCTACTCAGCAACAAAATCAGGCACAGGCAGAACAACAATGATCAAGGCGACGGACAGAAAACTGGTAGTAGGACTGGAGATTGGTACCGCGAAGGTTGCCGCTTTAGTAGGGGAAGTTCTGCCCGACGGTATGGTCAATATCATTGGCGTGGGCAGCTGCCCGTCGCGTGGTATGGATAAAGGCGGGGTGAACGACCTCGAAGCCG‑TGGTCAAGTGCGTACAAcg                                                                                                                                                                                                                      <  1:569/239‑1 (MQ=255)                           aaTCTACTCAGCAACAAAAGCAGGCACAGGCAGAACAACAATGATCAAGGCGACGGACAGAAAACTGGTAGTAGGACTGGAGATTGGTACCGCGAAGGTTGCCGCTTTAGTAGGGGAAGTTCTGCCCGACGGTATGGTCAATATCATTGGCGTGGGCAGCTGCCCGTCGCGTGGTATGGATAAAGGCGGGGTGAACGACCTCGAAGCCG‑TGGTCAAGTGCGTACAACGCGCCATTGa                                                                                                                                                                                                             <  2:305803/237‑1 (MQ=255)                              cTACTCAGCAACAAAATCAGGCACAGGCAGAACAACAATGATCAAGGCGACGGACAGAAAACTGGTAGTAGGACTGGAGATTGGTACCGCGAAGGTTGCCGCTTTAGTAGGGGAAGTTCTGCCCGACGGTATGGTCAATATCATTGGCGTGGGCAGCTGCCCGTCGCGTGGTATGGATAAAGGCGGGGTGAACGACCTCGAAGCCG‑TGGTCAAGTGCGTACAACGCGCCATTGACCAgg                                                                                                                                                                                                        <  1:680015/239‑1 (MQ=255)                                     gCAACAAAATCAGGCACAGGCAGAACAACAATGATCAAGGCGACGGACAGAAAACTGGTAGTAGGACTGGAGATTGGTACCGCGAAGGTTGCCGCTTTAGTAGGGGAAGTTCTGCCCGACGGTATGGTCAATATCATTGGCGTGGGCAGCTGCCCGTCGCGTGGTATGGATAAAGGCGGGGGGAACGACCTCGAAGCCG‑GGGGCAAGTGCGGACAACACGCCATTGACCAGGCAGAAtt                                                                                                                                                                                                 >  1:394566/1‑239 (MQ=255)                                                         caGAACAACAATGATCAAGGCGACGGACAGAAAACTGGTAGTAGGACTGGAGATTGGTACCGCGAAGGTTGCCGCTTTAGTAGGGGAAGTTCTGCCCGACGGTATGGTCAATATCATTGGCGTGGGCAGCTGCCCGTCGCGTGGTATGGATAAAGGCGGGGTGAACGACCTCGAAGCCG‑TGGTCAAGTGCGTACAACGCGCCATTGACCAGGCAGAATTGATGGCAGa                                                                                                                                                                                        <  1:22382/228‑1 (MQ=255)                                                         caGAACAACAATGATCAAGGCGACGGACAGAAAACTGGTAGTAGGACTGGAGATTGGTACCGCGAAGGTTGCCGCTTTAGTAGGGGAAGTTCTGCCCGACGGTATGGTCAATATCATTGGCGTGGGCAGCTGCCCGTCGCGTGGTATGGATAAAGGCGGGGTGAACGACCTCGAAGCCG‑TGGTCAAGTGCGTACAACGCGCCATTGACCAGGCAGAATTGATGGCAGATTGt                                                                                                                                                                                    <  1:773594/232‑1 (MQ=255)                                                         caGAACAACAATGATCAAGGCGACGGACAGAAAACTGGTAGTAGGACTGGAGATTGGTACCGCGAAGGTTGCCGCTTTAGTAGGGGAAGTTCTGCCCGACGGTATGGTCAATATCATTGGCGTGGGCAGCTGCCCGTCGCGTGGTATGGATAAAGGCGGGGTGAACGACCTCGAAGCCG‑TGGTCAAGTGCGTACAACGCGCCATTGACCAGGCAGAATTGATGGCAGATTGt                                                                                                                                                                                    <  1:244328/232‑1 (MQ=255)                                                           gAACAACAATGATCAAGGGGACGGACAGAAAACTGGTAGTAGGACTGGAGATTGGTAACGCGAAGGTTGCCGCTTTAGTAGGGGAAGTTCTGCCCGACGGTATGGTCAATATCATTGGCGTGGGCAGCTGCCCGTAGCGTGGTATGGATAAAGGCGGGGTGAACGACCTCGAAGCCG‑TGGTCAAGTGCGTACAACGCGGCATTGACCAGGCAGAATTGATGGCAGATTGTCAGAtctc                                                                                                                                                                            <  2:1091380/238‑1 (MQ=255)                                                             acaacaATGATCAAGGCGACGGACAGAAAACTGGTAGTAGGACTGGAGATTGGTACCGCGAAGGTTGCCGCTTTAGTAGGGGAAGTTCTGCCCGACGGTATGGTCAATATCATTGGCGTGGGCAGCTGCCCGTCGCGTGGTATGGATAAAGGCGGGGTGAACGACCTCGAAGCCG‑TGGTCAAGTGCGTACAACGCGCCATTGACCAGGCAGAATTGATGGCAGATTGTCAGAtctc                                                                                                                                                                            <  1:762663/236‑1 (MQ=255)                                                             acaacaATGATCAAGGCGACGGACAGAAAACTGGTAGTAGGACTGGAGATTGGTACCGCGAAGGTTGCCGCTTTAGTAGGGGAAGTTCTGCCCGACGGTATGGTCAATATCATTGGCGTGGGCAGCTGCCCGTCGCGTGGTATGGATAAAGGCGGGGTGAACGACCTCGAAGCCG‑TGGTCAAGTGCGTACAACGCGCCATTGACCAGGCAGAATTGATGGCAGATTGTCAGAtct                                                                                                                                                                             <  1:732540/235‑1 (MQ=255)                                                               aacaaTGATCAAGGCGACGGACAGAAAACTGGTAGTAGGACTGGAGATTGGTACCGCGAAGGTTGCCGCTTTAGTAGGGGAAGTTCTGCCCGACGGTATGGTCAATATCAGTGGCGTGGGCAGCTGCCCGTCGCGTGGTATGGATAAAGGCGGGGTGAACGACCTCGAAGCCG‑TGGTCAAGTGCGTACAACGCGCCATTGACCAGGCAGAATTGATGGCAGATTGTCAg                                                                                                                                                                                 <  1:1024322/229‑1 (MQ=255)                                                                acaaTGAACAAGGCGACGGACAGAAAACTGGTAGTAGGACTGGAGATTGGTACCGCGAAGGTTGCCGCTTTAGTAGGGGAAGTTCTGCCCGAAGGTATGGTCAATATCATTGGCGTGGGCAGCTGCCCGTCGCGTGGTATGGATAAAGGCGGGGTGAACGACCTCGAAGCCG‑TGGTCAAGTGCGTACAACGCGCCATTGACCAGGCAGAATTGATGGCAGATTGTCAGATCTCTTCGGt                                                                                                                                                                      <  2:1148123/239‑1 (MQ=255)                                                                  aaTGATCAAGGCGACGGACAGAAAACTGGTAGTAGGACTGGAGATTGGTACCGCGAAGGTTGCCGCTTTAGTAGGGGAAGTTCTGCCCGACGGTATGGTCAATATCATTGGCGTGGGCAGCTGCCCGTCGCGTGGTATGGATAAAGGCGGGGTGAACGACCTCGAAGCCG‑TGGTCAAGTGCGTACAACGCGCCATTGACCAGGCAGAATTGATGGCAGATTGTCAGATCTCTTCGGtat                                                                                                                                                                    >  1:214862/1‑239 (MQ=255)                                                                    tGATCAAGGCGACGGACAGAAAACTGGTAGTAGGACTGGAGATTGGTACCGCGAAGGTTGCCGCTTTAGTAGGGGAAGTTCTGCCCGACGGTATGGTCAATATCATTGGCGTGGGCAGCTGCCCGTCGCGTGGTATGGATAAAGGCGGGGTGAACGACCTCGAAGCCG‑TGGTCAAGTGCGTACAACGCGCCATTGACCAGGCAGAATTGATGGCAGAGTGTCAGATCTCTTCGGtata                                                                                                                                                                   >  1:467559/1‑238 (MQ=255)                                                                         aaGGCGACGGACAGAAAACTGGTAGTAGGACTGGAGATTGGTACCGCGAAGGTTGCCGCTTTAGTAGGGGAAGTTCTGCCCGACGGTATGGTCAATATCATTGGCGTGGGCAGCTGCCCGTCGCGTGGTATGGATAAAGGCGGGGTGAACGACCTCGAAGCCG‑TGGTCAAGTGCGTACAACGCGCCATTGACCAGGCAGAATTGATGGCAGATTGTCAGATCTCTTCGGTATAT‑CTGgc                                                                                                                                                            <  2:586491/239‑1 (MQ=255)                                                                         aaGGCGACGGACAGAAAACTGGTAGTAGGACTGGAGATTGGTACCGCGAAGGTTGCCGCTTTAGTAGGGGAAGTTCTGCCCGACGGTATGGTCAATATCATTGGCGTGGGCAGCTGCCCGTCGCGTGGTATGGATAAAGGCGGGGGGAACGACCTCGAAGCCG‑TGGGCAAGTGGGGACAAACCGCCCTTGACCAGGCAGAAATGATGGCAGATTGTCAGAACTCTTCGGTATAT‑CTGgc                                                                                                                                                            >  1:1119431/1‑239 (MQ=255)                                                                             cGACGGACAGAAAACTGGTAGTAGGACTGGAGATGGGTACCGCGAAGGTTGCCGCTTTAGTAGGGGAAGTTCTGCCCGACGGTATGGTCAATATCATTGGCGTGGGCAGCTGCCCGTCGCGTGGTATGGATAAAGGCGGGATGAACGACCTCGAAGCCG‑TGGTCAAGTGCGTACAACGCGCCATTAACCAGGCAGAATTGATGGCAGATTGTCAGATCTCTTCGGTATAT‑CGGGCGCt                                                                                                                                                         <  2:183441/238‑1 (MQ=255)                                                                                cGGACAGAAAACTGGTAGTAGGACTGGAGATTGGTACCGCGAAGGTTGCCGCTTTAGTAGGGGAAGTTCTGCCCGACGGTATGGTCAATATCATTGGCGTGGGCAGCTGCCCGTCGCGTGGTATGGATAAAGGCGGGGGGAACGACCTCGAAGCCG‑TGGTCAAGGGCGGACAAAGCGCCATTGACCAGGCCGAATTGATGGCGGATTGTCAGATTTCTTCGGGATAT‑CTggg                                                                                                                                                            >  2:60220/1‑231 (MQ=255)                                                                                  gACAGAAAACTGGTAGTAGGACTGGAGATTGGTACCGCGAAGGTTGCCGCTTTAGTAGGGGAAGTTCTGCCCGACGGTATGGTCAATATCATTGGCGTGGGCAGCTGCCCGTCGCGTGGTATGGATAAAGGCGGGGTGAACGACCTCGAAGCCG‑TGGTCAAGTGCGTa                                                                                                                                                                                                                           <  1:721263/168‑1 (MQ=255)                                                                                        aaaCTGGTAGTAGGACTGGAGATTGGTACCGCGAAGGTTGCCGCTTTAGTAGGGGAAGTTCTGCCCGACGGTATGGTCAATATCATTGGCGTGGGCAGCTGCCCGTCGCGTGGTATGGATAAAGGCGGGGTGAACGACCTCGAAGCCG‑TGGTCAAGTGCGTACAACGCGCCATTGACCAGGCAGAATTGATGGCAGATTGTCAGATCTCTTCGGTATAT‑CTGGCGCTTTCTGGTAAg                                                                                                                                               <  2:1167890/237‑1 (MQ=255)                                                                                            tGGTAGTAGGACTGGAGATTGGTACCGCGAAGGTTGCCGCTTTAGTAGGGGAAGTTCTGCCCGACGGTATGGTCAATATCATTGGCGTGGGCAGCTGCCCGTCCCGTGGGATGGATAAAGGCGGGGGGAACGACCTCGGAGCCG‑GGGTCAAGGGGGTACAAAGCGCCa                                                                                                                                                                                                                 >  2:721263/1‑168 (MQ=255)                                                                                                 gtagGACTGGAGATTGGTACCGCGAAGGTTGCCGCTTTAGTAGGGGAAGTTCTGCCCGACGGTATGGTCAATATCATTGGCGTGGGCAGCTGCCCGTCGCGTGGTATGGATAAAGGCGGGGTGAACGACCTCGAAGGCG‑TGGTCAAGGGCGGACAAAGCGCCATTGACCAGGCAGAATTGATGGCAGATTGTCAGATCTCTTCGGTATAT‑CTGgc                                                                                                                                                            >  1:158925/1‑215 (MQ=255)                                                                                                        tGGAGATTGGTACCGCGAAGGTTGCCGCTTTAGTAGGGGAAGTTCTGCCCGACGGTATGGTCAATATCATTGGCGTGGGCAGCTGCCCGTCGCGTGGTATGGATAAAGGCGGGGTGAACGGCCCCGAAGCCG‑GGGGCAAGTGCGTACAACGCGCCATTGGCCAGGCAGAATTGATGGCAGATTGTCAGATCTCTTCGGTATAT‑TCGGCGCTTTCTGGTAAGCACATACGCTGGCAGAAt                                                                                                                             >  2:320197/1‑239 (MQ=255)                                                                                                          gagaTTGGTACCGCGAAGGTTGCCGCTTTAGTAGGGGAAGTTCTGCCCGACGGTATGGTCAATATCATTGGCGTGGGCAGCTGCCCGTCGCGTGGTATGGATAAAGGCGGGGTGAACGACCTCGAAGCCG‑TGGTCAAGTGCGTACAACGCGCCATTGACCAGGCAGAAt                                                                                                                                                                                                  >  1:832216/1‑169 (MQ=255)                                                                                                          gagaTTGGTACCGCGAAGGTTGCCGCTTTAGTAGGGGAAGTTCTGCCCGACGGTATGGTCAATATCATTGGCGTGGGCAGCTGCCCGTCGCGTGGTATGGATAAAGGCGGGGTGAACGACCTCGAAGCCG‑TGGTCAAGTGCGTACAACGCGCCATTGACCAGGCAGAAt                                                                                                                                                                                                  <  2:832216/169‑1 (MQ=255)                                                                                                          gagaTTGGTACCGCGAAGGTTGCCGCTTTAGTAGGGGAAGTTCTGCCCGACGGTATGGGCAATATCATTGGCGTGGGCAGCTGCCCGTCGCGTGGTATGGATAAAGGCGGGGTGAACGACCTCGAAGCCG‑TGGTCAAGTGCGTACAACGCGCCATTGACCAGGCAGAATTGATGGCAGATTGGCAGATCTCTTCGGTATAT‑CTggg                                                                                                                                                            >  1:40113/1‑205 (MQ=255)                                                                                                           agaTTGGTACCGCGAAGGTTGCCGCTTTAGTAGGGGAGGTGCTGCCCGACGGTATGGTCAATATCATTGGCGTGGGCAGCTGCCCGTCGCGTGGTATGGATAAAGGCGGGGTGAACGACCTCGAAGCCG‑TGGTCAAGTGCGTACAACGCGCCATTGACCAGGCAGAATTGATGGCAGATTGTCAGATCTCTTCGGTATAT‑CTGGCGCTTTCTGGTAAGCACATCAGCTGCCAGaa                                                                                                                              <  1:911836/235‑1 (MQ=255)                                                                                                             aTTGGTACCGCGAAGGTTGCCGCTTTAGTAGGGGAAGTTCTGCCCGACGGTATGGTCAATATCATTGGCGTGGGCAGCTGCCCGTCGCGTGGTATGGCTAAAGGCGGGGGGGAAGACGTCGCAGCCG‑GGGGCAAGTGGGGACCACGCGCCATTGACCAGGCAGAATTTATGGCATATTGTCAGGTCTCTTCGGGATAA‑CTGGCGCTTTCTGGTAAGCACCTCAGCAGCCACAAGGaaaa                                                                                                                        >  2:58936/1‑238 (MQ=255)                                                                                                               tGGTACCGCGAAGGTTGCCGCTTTAGTAGGGGAAGTTCTGCCCGACGGTATGGTCAATATCATTGGCGTGGGCAGCTGCCCGTCGCGTGGTATGGATAAAGGCGGGGTGAACGACCTCGAAGCCG‑TGGTCAAGTGCGTACAACGCGCCATTGACCAGGCAGAATTGATGGCAGATTGTCAGATCTCTTCGGTATAT‑CTGgc                                                                                                                                                            >  1:804673/1‑201 (MQ=255)                                                                                                                   aCCGCGGAGGTTGCCGCTTTAGTAGGGGAAGTTCTGCCCGACGGTATGGTCAATATCATTGGCGTGGGCAGCTGCCCGTCGCGTGGTATGGATAAAGGCGGGGTGAACGACCTCGAAGCCG‑TGGTCAAGTGCGTACAACGCGCCATTGACCAGGCAGAATTGATGGCAGATTGTCAGATCTCTTCGGTATAT‑CTGGCGCTTTCTGGTAAGCACATCAGCTGCCAGAATGAAATTGGTa                                                                                                                   <  1:404583/238‑1 (MQ=255)                                                                                                                    ccGCGACGGTTGCCGCTTTAGTAGGGGAAGTTCTGCCCGACGGTATGGGCAATATCAGTGGCGGGGGCAGCTGCCCGTCGCGTGGTATGGAAAAAGGCGGGGTGAACGGCCTCGAAGCCC‑TGGTCGAATGCGGACAAAGCGGCAATGGCCCGGCAAGATTGAGGGGAAAATGGCACACCGCTTCGG‑ATATAATGGCGCTTTGCGGGAAGCACAGCAACTGCCAGCAGAAAATTggggag                                                                                                                 >  2:721678/1‑235 (MQ=255)                                                                                                                    ccGCGAAGGTTGCCGCTTTAGTAGGGGAAGTTCTGCCCGACGGTATGGTCAATATCATTGGCGTGGGCAGCTGCCCGTCGCGTGGTATGGATAAAGGCGGGGTGAACGACCTCGAAGCCG‑TGGTCAAGTGCGTACAACGCGCCATTGACCAGGCAGAATTGATGGCAGATTGTCCGATCTCTTCGGTATAT‑CTGgc                                                                                                                                                            >  1:967760/1‑196 (MQ=255)                                                                                                                            gTTGCCGCTTTAGTAGGGGAAGTTCTGCCCGACGGTATGGTCAATATCATTGGCGTGGGCAGCTGCCCGTCGCGTGGTATGGATAAAGGCGGGGTGAACGACCTCGAAGCCG‑TGGTCAAGTGCGTACAACGCGCCATTGACCAGGCAGAATTGATGGCAGATTGTCAGATCTCTTCGGTATAT‑CTGGCGCTTTCTg                                                                                                                                                    <  2:967760/196‑1 (MQ=255)                                                                                                                             ttGCCGCTTTAGTAGGGGAAGTTCTGCCCGACGGTATGGTCAATATCATTGGCGTGGGCAGCTGCCCGTCGCGTGGTATGGATAAAGGCGGGGTGAACGACCTCGAAGCCG‑TGGTCAAGTGCGTACAACGCGCCATTGACCAGGCAGAATTGATGGCAGATTGTCAGATCTCTTCGGTATAT‑CTGGCGCTTTCTGGTAAGCACATCAGCTGCCAGAATGAAATTGGTATGGTGCCTAt                                                                                                         <  2:214862/238‑1 (MQ=255)                                                                                                                                              gAAGTTCTGCCCGACGGTATGGTCAATATCATTGGCGTGGGCAGCTGCCCGTCGCGTGGTATGGATAAAGGCGGGGGGAACGACCTCGAAGCCG‑TGGTCAAGTGCGTACAACGCGCCATTGACCAGGCAGAATTGATGGCAGATTGTCAGATCTCTTCGGTATAT‑CTGGCGCTTTCTGGTAAGCACATCAGCTGCCAGAATGAAATTGGTATGGTGCCTATTTCTGAAGAAGAAGTGAc                                                                                       >  2:652713/1‑239 (MQ=255)                                                                                                                                                  ttCTGCCCGACGGTATGGTCAATATCATTGGCGTGGGCAGCTGCCCGTCGCGTGGTATGGATAAAGGCGGGGTGAACGACCTCGAAGCCG‑TGGTCAAGTGCGTACAACGCGCCATTGACCAGGCAGAATTGATGGCAGATTGt                                                                                                                                                                                    <  2:411874/143‑1 (MQ=255)                                                                                                                                                  ttCTGCCCGACGGTATGGTCAATATCATTGGCGTGGGCAGCTGCCCGTCGCGTGGTATGGATAAAGGCGGGGTGAACGACCTCGAAGCCG‑TGGTCAAGTGCGTACAACGCGCCATTGACCAGGCAGAATTGATGGCAGATTGt                                                                                                                                                                                    >  1:411874/1‑143 (MQ=255)                                                                                                                                                       cccGACGGTATGGTCAATATCATTGGCGTGGGCAGCTGCCCGTCGCGTGGTATGGATAAAGGCGGGGTGAACGACCTCGAAGCCG‑TGGTCAAGTGCGTACAACGCGCCATTGACCAGGCAGAATTGATGGCAGa                                                                                                                                                                                        >  2:844749/1‑134 (MQ=255)                                                                                                                                                       cccGACGGTATGGTCAATATCATTGGCGTGGGCAGCTGCCCGTCGCGTGGTATGGATAAAGGCGGGGTGAACGACCTCGAAGCCG‑TGGTCAAGTGCGTACAACGCGCCATTGACCAGGCAGAATTGATGGCAGa                                                                                                                                                                                        <  1:844749/134‑1 (MQ=255)                                                                                                                                                       cccGACGGTATGGTCAATATCATTGGCGTGGGCAGCTGCCCGTCGCGTGGTATGGATAAAGGCGGGGTGAACGACCTCGAAGCCG‑TGGTCAAGTGCGTACAACGCGCCATTGACCAGGCAGAATTGATGGCAGATTGTCAGAtctc                                                                                                                                                                            >  2:423332/1‑146 (MQ=255)                                                                                                                                                       cccGACGGTATGGTCAATATCATTGGCGTGGGCAGCTGCCCGTCGCGTGGTATGGATAAAGGCGGGGTGAACGACCTCGAAGCCG‑TGGTCAAGTGCGTACAACGCGCCATTGACCAGGCAGAATTGATGGCAGATTGTCAGAtctc                                                                                                                                                                            <  1:423332/146‑1 (MQ=255)                                                                                                                                                            cGGTATGGTCAATATCATTGGCGTGGGCAGCTGCCCGTCTCGTGGTATGGATAAAGGCGGGGGGAACGACCTCGAAGCCG‑TGGTCAAGTGCGTACAACGCGCCATTGACCAGGCAGAATTGATGGCAGATTGTCAGATCTCTTCGGTATAT‑CTGGCGCTTTTTGGTAAGCACATCAACTGCCAGAAAGAAATTTGTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTgg                                                                         >  2:167690/1‑239 (MQ=255)                                                                                                                                                                    tCAATATCATTGGCGTGGGCAGCTGCCCGTCGCGTGGTATGGATAAAGGCGGGGGGGACGACCTCGAAGCCG‑TGGGCAAGTGCGTACAACGCGCCATTGACCAGGCAGAATTGATGGCAGATTGTCAGATCTTTTCGGTATAT‑CTGGCGCTTTCTGGTAAGAACATCAGCTGACCGCATGAAATTGGTATGGTGCCTATTTCTGAAGCAGAAGTGACGCCAGAAGATTGTGAAAAcgtc                                                                 >  2:841296/1‑239 (MQ=255)                                                                                                                                                                         atCATTGGCGTGGGCAGCTGCCCGTCGCGTGGTATGGATAAAGGCGGGGTGAACGACCTCGAAGCCG‑TGGt                                                                                                                                                                                                                                     <  1:1048953/71‑1 (MQ=255)                                                                                                                                                                         atCATTGGCGTGGGCAGCTGCCCGTCGCGTGGTATGGATAAAGGCGGGGGGAACGACCTCGAAGCCG‑TGGt                                                                                                                                                                                                                                     >  2:1048953/1‑71 (MQ=255)                                                                                                                                                                           cATTGGCGTGGGCAGCTGCCCGTCGCGTGGTATGGATAAAGGCGGGGTGAACGACCTCGAAGCCG‑TGGTCAAGTGCGTACAACGCGCCATTGACCAGGCAGAATTGATGGCAGATTGTCAg                                                                                                                                                                                 <  1:56620/121‑1 (MQ=255)                                                                                                                                                                             ttGGCGTGGGCAGCTGCCCGTCGCGTGGTATGGATAAAGGCGGGGGGAACGACCTCGAAGCCG‑TGGTCAAGTGCGTACAACGCGCCATTGACCAGGCAGAATTGATGGCAGATTGTCAGATCTCTTCGGTATAT‑CTGgc                                                                                                                                                            >  1:254745/1‑139 (MQ=255)                                                                                                                                                                                gCGTGGGCAGCTGCCCGTCGCGTGGTATGGATAAAGGCGGGGTGAACGACCTCGAAGCCG‑TGGTCAAGTGCGTACAACGCGCCATTGACCAGGCAGAATTGATGGCAGATTGTCAGATCTCTTCGGTATAT‑CTGGCGCTTTCTGGTAAGCACATCAGCTGCCAGAATGAAATTGGTATGGTGCCTATTTc                                                                                                      <  1:761991/190‑1 (MQ=255)                                                                                                                                                                                gCGTGGGCAGCTGCCCGTCGCGTGGTATGGATAAAGGCGGGGTGAACGACCGCGAAGCCG‑TGGTCAAGTGCGTACAACGCGCCATTGACCAGGCAGAATTGATGGCAGATTGTCAGATCTCTTCGGTATAT‑CTGGCGCTTTCTGGTAAGCACATCAGCTGCCAGAATGAAATTGGTATGGTGCCTATTTCTGAAGAAGAAGTGAc                                                                                       <  1:1082206/205‑1 (MQ=255)                                                                                                                                                                                     ggCAGCTGCCCGTCGCGTGGGATGGATAAAGGCGGGGGGAAAGACCTCGAAGCCG‑GGGGCAAGGGGGGACAACGCGCCATTGACCAGGCCGAATTGATGGCAGATTGTCAGATCTCCTCgg                                                                                                                                                                       >  2:56620/1‑121 (MQ=255)                                                                                                                                                                                          cTGCCCGTCGCGTGGTATGGATAAAGGCGGGGTGAACGACCTCGAAGCCG‑TGGTCAAGTGCGTACAACGCGCCATTGACCAGGCAGAATTGATGGCAGa                                                                                                                                                                                        <  1:481261/99‑1 (MQ=255)                                                                                                                                                                                          cTGCCCGTCGCGTGGTATGGATAAAGGCGGGGTGAACGACCTCGAAGCCG‑TGGTCAAGTGCGTACAACGCGCCATTGACCAGGCAGAATTGATGGCAGa                                                                                                                                                                                        >  2:481261/1‑99 (MQ=255)                                                                                                                                                                                          cTGCCCGTCGCGTGGTATGGATAAAGGCGGGGTGAACGACCTCGAAGCCG‑TGGTCAAGTGCGTACAACGCGCCATTGACCAGGCAGAATTGATGGCAGATTGTCAGATCTCTTCGGTATAT‑CTGgc                                                                                                                                                            >  2:1082206/1‑126 (MQ=255)                                                                                                                                                                                          cTGCCCGTCGCGTGGTATGGATAAAGGCGGGGGGAACGACCTCGAAGCCG‑TGGTCAAGTGCGTACAACGCGCCATTGACCAGGCAGAATTGATGGCAGATTGTCAGATCTCTTCGGTATAT‑CTGgc                                                                                                                                                            >  2:761991/1‑126 (MQ=255)                                                                                                                                                                                                  cgcgTGGTATGGATAAAGGCGGGGTGAACGACCTCGAAGCCG‑TGGTCAAGTGCGTACAACGCGCCATTGACCAGGCAGAATTGATGGCAGATTGTCAGAtctc                                                                                                                                                                            >  2:691561/1‑103 (MQ=255)                                                                                                                                                                                                  cgcgTGGTATGGATAAAGGCGGGGTGAACGACCTCGAAGCCG‑TGGTCAAGTGCGTACAACGCGCCATTGACCAGGCAGAATTGATGGCAGATTGTCAGAtctc                                                                                                                                                                            <  1:691561/103‑1 (MQ=255)                                                                                                                                                                                                     gTGGTATGGATAAAGGCGGGGTGAACGACCTCGAAGCCG‑TGGTCAAGTGCGTACAACGCGCCATTGACCAGGCAGAATTGATGGCAGATTGTCAGATCTCTTCGGTATAT‑CTGGCGCTTTCTGGTAAg                                                                                                                                               <  1:513179/128‑1 (MQ=255)                                                                                                                                                                                                       ggTAGGGATAAAGGCGGGGTGAACGAACTCGAAGCCG‑TGGTCAAGTGCGTACAACGCGCCATTGACCAGGCAGAATTGATGGCAGATTGTCAGATCTCTTCGGTATAT‑CTGGCGCTTTCTGGTAAGCACATCAGCTGCCAGAATGAAATTGGTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGa                              <  2:1072011/239‑1 (MQ=255)                                                                                                                                                                                                         tATGGATAAAGGCGGGGTGAACGACCTCGAAGCAG‑TGGTCAAGTGCGTACAACGCGCCATTGACCAGGCAGAATTGATGGCAGATTGTCAGATCTCTTCGGTATAT‑CTGGCGCTTTCTGGTAAGCACATCAGCTGCCAGAATGAAATTGGTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAAc                                                                    <  1:625371/199‑1 (MQ=255)                                                                                                                                                                                                          aTGGATAAAGGCGGGGTGAACGGCCTAGAAGCCG‑TGGTCAAGTGCGTACAACGCGCCATTGACCAGGCAGAATTGATGGCAGATTGTCAGATCTCTTCGGTATAT‑CTGGCGCTTTCTGGTAAGCACATCAGCTGCCAGAATGAAATTGGTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGAGc                            <  2:11913/238‑1 (MQ=255)                                                                                                                                                                                                           tGGATAAAGGCGGGGTGAACGACCTCGAAGCCG‑TGGTCAAGTGCGTACAACGCGCCATTGACCAGGCAGAATTGATGGCAGATTGTCAGATCTCTTCGGTATAT‑CTGgc                                                                                                                                                            >  1:1171679/1‑109 (MQ=255)                                                                                                                                                                                                             gATAAAGGCGGGGTGAACGACCTCGAAGCCG‑TGGTCAAGTGCGTACAACGCGCCATTGACCAGGCAGAATTGATGGCAGATTGTCAGATCTCTTCGGTATAT‑CTGGCGCTTTCTGGTAAGCACATCAGCTGCCAGAATGa                                                                                                                           <  1:372908/140‑1 (MQ=255)                                                                                                                                                                                                               tAAAGGCGGGGTGAACGACCTCGAAGCCG‑TGGTCAAGTGCGTACAACGCGCCATTGACCAGGCAGAATTGATGGCAGATTGTCAGATCTCTTCGGTATAT‑CTGgc                                                                                                                                                            >  2:513179/1‑105 (MQ=255)                                                                                                                                                                                                                   ggCGGGGTGAACGACCTCGAAGCCG‑TGGTCAAGTGCGTa                                                                                                                                                                                                                           >  2:790207/1‑39 (MQ=255)                                                                                                                                                                                                                   ggCGGGGTGAACGACCTCGAAGCCG‑TGGTCAAGTGCGTa                                                                                                                                                                                                                           <  1:790207/39‑1 (MQ=255)                                                                                                                                                                                                                   ggCGGGGTGAACGACCTCGAAGCCG‑TGGTCAAGTGCGTACAACGCGCCATTGACCAGGCAGAATTGATGGCAGATTGTCAGATCTCTTCGGTATAT‑CTGgc                                                                                                                                                            >  1:153094/1‑101 (MQ=255)                                                                                                                                                                                                                   ggCGGGGTGAACGACCTCGAAGCCG‑TGGTCAAGTGCGTACAACGCGCCATTGACCAGGCAGAATTGATGGCAGATTGTCAGATCTCTTCGGTATAT‑CTGgc                                                                                                                                                            >  2:625371/1‑101 (MQ=255)                                                                                                                                                                                                                   ggCGGGGTGAACGACCTCGAAGCCG‑TGGTCAAGAGCGTACAACGCGCCATTGACCAGGCAGAATTGATGGCAGATTGTCAGATCTCTTCGGTATAT‑CTGgc                                                                                                                                                            >  2:119182/1‑101 (MQ=255)                                                                                                                                                                                                                     cGGGGTGAACGACCTCGAAGCCG‑TGGTCAAGTGCGTACAACGCGCCATTGACCAGGCAGAATTGATGGCAGATTGTCAGATCTCTTCGGTATAT‑CTGGCGCTTTCTGGTAAGCACATCAGCTGCCAGAATGAAATTGGTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGAGCATCGtgtg                    <  1:58936/235‑1 (MQ=255)                                                                                                                                                                                                                       gggTGAACGACCTCGAAGCCG‑TGGTCAAGTGCGTACAACGCGCCATTGACCAGGCAGAATTGATGGCAGATTGTCAg                                                                                                                                                                                 >  2:157263/1‑77 (MQ=255)                                                                                                                                                                                                                       gggTGAACGACCTCGAAGCCG‑TGGTCAAGTGCGTACAACGCGCCATTGACCAGGCAGAATTGATGGCAGATTGTCAg                                                                                                                                                                                 <  1:157263/77‑1 (MQ=255)                                                                                                                                                                                                                       gggTGAACGACCTCGAAGCCG‑TGGTCAAGTGCGTACAACGCGCCATTGACCAGGCAGAATTGATGGCAGATTGTCAGATCTCTTCGGTATAT‑CTGgc                                                                                                                                                            >  1:1036265/1‑97 (MQ=255)                                                                                                                                                                                                                       gggTGAACGACCTCGAAGCCG‑TGGTCAAGTGCGTACAACGCGCCATTGACCAGGCAGAATTGATGGCAGATTGTCAGATCTCTTCGGTATAT‑CTGgc                                                                                                                                                            >  1:669299/1‑97 (MQ=255)                                                                                                                                                                                                                       gggTGAACGACCTCGAAGCCG‑TGGTCAAGTGCGTACAACGCGCCATTGACCAGGCAGAATTGATGGCAGATTGTCAGATCTCTTCGGTATAT‑CTGgc                                                                                                                                                            >  2:437262/1‑97 (MQ=255)                                                                                                                                                                                                                       gggTGAACGACCTCGAAGCCG‑TGGTCAAGTGCGTACAACGCGCCATTGACCAGGCAGAATTGATGGCAGATTGTCAGATCTCTTCGGTATAT‑CTGgc                                                                                                                                                            >  2:372908/1‑97 (MQ=255)                                                                                                                                                                                                                       gggTGAACGACCTCGAAGCCC‑TGGTCAAGTGCGTACAACGCGCCATTGACCAGGCAGAATTGATGGCAGATTGTCAGATCTCTTCGGTATAT‑CTGgc                                                                                                                                                            >  2:890077/1‑97 (MQ=255)                                                                                                                                                                                                                        ggTGAACGACCTCGAAGCCG‑TGGTCAAGTGCGTACAACGCGCCATTGACCAGGCAGAATTGATGGCAGATTGTCAGATCTCTTCGGTATAT‑CTGGCGCTTTCTGGTAAGCACATCAGCTGCCAGAATGa                                                                                                                           <  1:1173985/129‑1 (MQ=255)                                                                                                                                                                                                                                 ccTCGAAGCCG‑TGGTCAAGTGCGTACAACGCGCCATTGACCAGGCAGAATTGATGGCAGATTGTCAGATCTCTTCGGTATAT‑CTGGCGCTTTCTGGTAAGCACATCAGCTGCCAGAATGAAATTGGTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGAGCATCGTGTGCTGCATGTGATCCCGc    <  1:60220/239‑1 (MQ=255)                                                                                                                                                                                                                                  cTCGAAGCCG‑TGGTCAAGTGCGTACAACGCGCCATTGACCAGGCAGAATTGATGGCAGATTGTCAGATCTCTTCGGTATAT‑CTGgc                                                                                                                                                            >  2:1173985/1‑86 (MQ=255)                                                                                                                                                                                                                                  cTCGAAGCCG‑TGGTCAAGTGCGTACAACGCGCCATTGACCAGGCAGAATTGATGGCAGATTGTCAGATCTCTTCGGTATAT‑CTGGCGCTTTCTGGTAAGCACATCAGCTGCCAGAATGAAATTGGTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAAc                                                                    <  1:661100/174‑1 (MQ=255)                                                                                                                                                                                                                                   tCGAAGCCG‑TGGTCAAGTGCGTACAACGCGCCATTGACCAGGCAGAATTGATGGCAGATTGTCAGATCTCTTCGGTATAT‑CTGGCGCTTTCTGGTAAGCACATCAGCTGCCAGAATGAAATTGGTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGAGCATCGTGTGCTGCATGTGATCCCGCaa  <  2:158925/239‑1 (MQ=255)                                                                                                                                                                                                                                   tCGAAGCCG‑GGGTCAAGTGCGTACAACGCGCCATTGACCAGGCAGAATTGATGGCAGATTGTCAGATCTCTTCGGTATAT‑CTGGCGCTTTCTGGTAAGAACATCAGCTGCCAGAATGAAATTGGTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGAGCATCGTGTGCTGCATGTGATCCCGCaa  <  2:804673/239‑1 (MQ=255)                                                                                                                                                                                                                                    cGAAGCCG‑TGGTCAAGTGCGTACAACGCGCCATTGACCAGGCAGAATTGATGGCAGATTGTCAGATCTCTTCGGTATAT‑CTGgc                                                                                                                                                            >  1:56534/1‑84 (MQ=255)                                                                                                                                                                                                                                    cGAAGCCG‑TGGTCAAGTGCGTACAACGCGCCATTGACCAGGCAGAATTGATGGCAGATTGTCAGATCTCTTCGGTATAT‑CTGGCGCTTTCTGGTAAGCACATCAGCTGc                                                                                                                                   <  2:1171679/109‑1 (MQ=255)                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                            AGGCTGGGCGCCCTTGCCGCCAGAGGAATCTACTCAGCAACAAAATCAGGCACAGGCAGAACAACAATGATCAAGGCGACGGACAGAAAACTGGTAGTAGGACTGGAGATTGGTACCGCGAAGGTTGCCGCTTTAGTAGGGGAAGTTCTGCCCGACGGTATGGTCAATATCATTGGCGTGGGCAGCTGCCCGTCGCGTGGTATGGATAAAGGCGGGGTGAACGACCTCGAATCCG‑TGGTCAAGTGCGTACAACGCGCCATTGACCAGGCAGAATTGATGGCAGATTGTCAGATCTCTTCGGTATAT‑CTGGCGCTTTCTGGTAAGCACATCAGCTGCCAGAATGAAATTGGTATGGTGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGAGCATCGTGTGCTGCATGTGATCCCGCAA  >  NC_000913/103916‑104380

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑