Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	243,021	T→G	T95P (ACC→CCC)	fadE ←	acyl coenzyme A dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	243,021	0	T	G	100.0%	223.2 / NA	72	T95P (ACC→CCC)	fadE	acyl coenzyme A dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (29/43);  total (29/43)

GAAGAAACGATGCTCTTTAAGGTACGCCCACAACTCCGGCGGCAGATCCGCCAGCTCATGGGTGATCTGGAAATCATTCGCCATCCGGCAGGCTTCTTCTACCGGGCCGTCGAGAAACGCTTGCTCTTCGGCGGTCAGGCGCGGCTGCGGATAGTTATGCAGCTTTTTCCAGTCCGGCTTGCCCTGGAACAAGTCGCCCTCCCACCAGGTGGTGCCCGCATCAATCGCTTCTTTCTCA‑GTGCGCGACATCGGCGGCATCACCTTACGGAAACCGCGAAATACCGGCGCGGAAATCATCGACTTACGCATAGGCGCAAAGTTAAATGGCACGAGGATAATGGCCAGAGGCACCAGTACCCACGCCGACCACAGACCAGCAACGCCGAGGGCGGCTGTCCAGGCGAGCAAAATCAGACTGCTGATAAATAAGCTCACGCGGTGAT  >  NC_000913/242809‑243251                                                                                                                                                                                                                     |                                                                                                                                                                                                                                        gaagaaACGATGCTTTTTAAGGTACGCCCACAACTCCGGCGGCAGATCCGCCAGCTCATGGGTGATCTGGAAATCATTCGCCATCCGGCAGGCTTCTTCTACCGGGCCGTCGAAAAACGCTTGCTCTTCGGCGGTCAGGCGCGGCTGCGGATAGTTATGCAGCTTTTTCCAGTCCGGCTTGCCCTGGAACAAGTCGCCCTCCCACCAGGTGGGGCCCGCATCAATCGCttctttct                                                                                                                                                                                                                  <  1:566566/236‑1 (MQ=255)             ctctTTAAGGTACGCCCACAACTCCGGCGGCAGATCCGCCAGCTCATGGGTGATCTGGAAATCATTCGCCATCCGGCAGGCTTCTTCTACCGGGCCGTCGAGAAACGCTTGCTCTTCGGCGGTCAGGCGCGGCTGCGGATAGTTATGCAGCTTTTTCCAGTCCGGCTTGCCCTGGAACAAGTCGCCCTCCCACCAGGTGGGGCCCGCATCAATCGCTTCTTTCTCA‑GTGCGCGACa                                                                                                                                                                                                     >  1:741167/1‑236 (MQ=255)                      gcaCGCCAACATCTCGCGCGGCAGATCCGCCAGCTCATGGGTGATCTGGAAATCATTCGCCATCCGGCAGGCTTCTTCTACCGGGCCGTCGAGAAACGCTTGCTCTTCGGCGGTCAGGCGCGGCTGCGGATAGTTATGCAGCTTTTTCCAGTCCGGCTTGCCCTGGAACAAGTCGCCCTCCCACCAGGTGGGGCCCGCATCAATCGCTTCTTTCTCA‑GTGCGCGACATCGGCGGCa                                                                                                                                                                                            <  2:243284/234‑1 (MQ=255)                      gTACGCCCACAACTCCGGCGGAAGATCCGCCAGCTCATGGGTGATCTGGAAATCATTCGCCATCCGGCAGGCTTCTTCTACCGGGCCGTCGAGAAACGCTTGCTCTTCGGCGGTCAGGCGCGGCTGCGGATAGTTATGCAGCTTTTTCCAGTCCGGCTTGCCCTGGAACAAGTCGCCCTCCCACCAGGTGGGGCCCGCATCAATCGCTTCTTTCTCA‑GTGCGCGACATCGGCGGCa                                                                                                                                                                                            <  1:135048/236‑1 (MQ=255)                      gTACGCCACCAACCCCGTCGGCAGATCCGCCAGCTCATGGGTTATCTGGAAATCATTCGCCATCCGGCAGGCTTCTTCTACCGGGCCGTCGAGAAACGCTTGCTCTTCGGCGGTCAGGCGCGGCTGCGGATAGTTATGCAGCTTTTTCCAGTCCGGCTTGCCCTGGAACAAGTCGCCCTCCCACCAGGTGGGGCCCGCATCAATCGCTTCTTTCTCA‑GTGCGCGACATCGGCGGCa                                                                                                                                                                                            <  2:741167/236‑1 (MQ=255)                       tACGCCCACAACTCCGGCGGCAGATCCGCCAGCTCATGGGTGATCTGGAAATCATTCGCCATCCGGCAGGCTTCTTCTACCGGGCCGTCGAGAAACGCTTGCTCTTCGGCGGTCAGGCGCGGCTGCGGATAGTTATGCAGCTTTTTCCAGTCCGGCTTGCCCTGGAACAAGTCGCCCTCCCACCAGGTGGGGCCCGCATCAATCGCTTCTTTCTCA‑GTGCGCGACATCGGCGGCAt                                                                                                                                                                                           <  1:648722/236‑1 (MQ=255)                          gCCCACAACTCCGGCGGCAGATCCGCCAGCTCATGGGTGATCTGGAAATCATTCGCCATCCGGCAGGCTTCTTCTACCGGGCCGTCGAGAAACGCTTGCTCTTCGGCGGTCAGGCGCGGCTGCGGATAGTTATGCAGCTTTTTCCAGTCCGGCTTGCCCTGGAACAAGTCGCCCTCCCACCAGGTGGGGCCCGCATCAATCGCTTCTTTCTCA‑GTGCTCGACAAtggcgggctca                                                                                                                                                                                         >  2:719107/1‑230 (MQ=255)                          gCCCACAACTCCGGCGGCAGATCCGCCAGCTCATGGGTGATCTGGAAATCATTCGCCATCCGGCAGGCTTCTTCTACCGGGCCGTCGAGAAACGCTTGCTCTTCGGCGGTCAGGCGCGGCTGCGGATAGTTATGCAGCTTTTTCCAGTCCGGCTTGCCCTGGAACAAGTCGCCCTCCCACCAGGTGGGGCCCGCATCAATCGCTTCTTTCTCA‑GTGCGCGACATCGGCGGCATCAc                                                                                                                                                                                        >  1:166532/1‑236 (MQ=255)                          gCCCACAACTCCGGCGGCAGATCCGCCAGCTCATGGGTGATCTGGAAATCATTCGCCATCCGGCAGGCTTCTTCTACCGGGCCGTCGAGAAACGCTTGCTCTTCGGCGGTCAGGCGCGGCTGCGGATAGTTATGCAGCTTTTTCCAGTCCGGCTTGCCCCGGGACAAGTCGCCCTCCCACCAGGTGGGGCCCGCATCCATCGCTTCCTTCTCA‑GTGCGCGTCATCggcggc                                                                                                                                                                                             >  2:218527/1‑231 (MQ=255)                          gCCCACAACTCCGGCGGCAGATCCGCCAGCTCATGGGTGATCTGGAAATCATTCGCCATCCGGCAGGCTTCTTCTACCGGGCCGTCGAGAAACGCTTGCTCTTCGGCGGTCAGGCGCGGCTGCGGATAGGTATGCAGCTTTTTCCAGTCCGGCTTGCCCCGGAACAAGTCGCCCTCCCACCAGGTGGGGCCCGCACCAACCGCTTCTTTCTCA‑TAGCGCGACCTCGGCGGCATccc                                                                                                                                                                                        >  2:738832/1‑234 (MQ=255)                           cccAGAACTCCGTCGGCAGATCCGCCAGCTCATGGGTGATCTGGAAACCATTCGCCATCCGGCAGGCTTCTTCGACCGGGCCGTCGAGAAACGCTTGCTCTTCGGCGGTCAGGCGCGGCTGCGGATAGTTATGCAGCTTTTTCCAGTCCGGCTTGCCCTGGAACAAGTCGCCCTCCCACCAGGTGGGGCCCGCATCAATCGCTTCTTTCTCA‑GTGCGCGACATCGGCGGCATCAc                                                                                                                                                                                        <  2:429163/235‑1 (MQ=255)                           accACAACTCCGGCGGCAGATCCGCCAGCTCATGGGTGATCTGGAAATCATTCGCCATCCGGCAGGCTTCTTCTACCGGGCCGTCGAGAAACGCTTGCTCTTCGGCGGTCAGGCGCGGCTGCGGATAGTTATGCAGCTTTTTCCAGTCCGGCTTGCCCTGGAACAAGTCGCCCTCCCACCAGGTGGGGCCCGCATCAATCGCTTCTTTCTCA‑GTGCGCGACATCGGCGGCATCAc                                                                                                                                                                                        <  1:730719/234‑1 (MQ=255)                                  cTCCGGCGGCAGATCCGCCAGCTCATGGGTGATCTGGAAATCATTCGCCATCCGGCAGGCTTCTTCTACCGGGCCGTCGAGAAACGCTTGCTCTTCGGCGGTCAGGCGCGGCTGCGGATAGTTATGCAGCTTTTTCCAGTCCGGCTTGCCCTGGAACAAGTCGCCCTCCCACCAGGTGGGGCCCGCATCAATCGCTTCTTTCTCA‑GTGCGCGACATCGGCGGCATCACCTTACGGa                                                                                                                                                                                <  1:209660/236‑1 (MQ=255)                                    ccGGGGGCAGTTCCGCCAGCTCATGGGTGATCTGGAAATCATTCGCCATCCGGCAGGCTTCTTCTACCGGGCCGTCGAGAAACGCTTGCTCTTCGGCGGTCAGGCGCGGCTGCGGATAGTTATGCAGCTTTTTCCAGTCCGGCTTGCCCTGGAACAAGTCGCCCTCCCACCAGGTGGGGCCCGCATCAATCGCTTCTTTCTCA‑GTGCGCGACATCGGCGGCATCACCTTACGGaaa                                                                                                                                                                              <  1:719107/236‑1 (MQ=255)                                     cggcggCAGATCCGCCAGCTCATGGGTGATCTGGAAATCATTCGCCATCCGGCAGGCTTCTTCTACCGGGCCGTCGAGAAACGCTTGCTCTTCGGCGGTCAGGCGCGGCTGCGGATAGTTATGCAGCTTTTTCCAGTCCGGCTTGCCCTGGAACAAGTCGCCCTCCCACCAGGTGGGGCCCGCATCCATCGCTTCTTTCTCC‑GTGCGCGACATCGACGGGCTCAACCTACGGCAAc                                                                                                                                                                             >  2:419965/1‑236 (MQ=255)                                     cggcggCAGATCCGCCAGCTCATGGGTGATCTGGAAATCATTCGCCATCCGGCAGGCTTCTTCTACCGGGCCGTCGAGAAACGCTTGCTCTTCGGCGGTCAGGCGCGGCTGCGGATAGTTATGCAGCTTTTTCCAGTCCGGCTTGCCCTGGAACAAGTCGCCCTCCCACCAGGTGGGGCCCGCATCAATCGCTTCTTTCTTC‑GTGCGCGCCATCGGCGGCATCCCCTTACGGGAAc                                                                                                                                                                             >  2:707453/1‑236 (MQ=255)                                     cggcggCAGATCCGCCAGCTCATGGGTGATCTGGAAATCATTCGCCATCCGGCAGGCTTCTTCTACCGGGCCGTCGAGAAACGCTTGCTCTTCGGCGGTCAGGCGCGGCTGCGGATAGTTATGCAGCTTTTTCCAGTCCGGCTTGCCCTGGAACAAGTCGCCCTCCCACCAGGTGGGGCCCGCATCAATCGCTTCTTTCTCA‑GTGGGCGGCATCGGCGGCATCACCTTACGGGAAc                                                                                                                                                                             >  2:521429/1‑236 (MQ=255)                                                  gCCAGCTCATGGGTGATCTGGAAATCATTCGCCATCCGGCAGGCTTCTTCTACCGGGCCGTCGAGAAACGCTTGCTCTTCGGCGGTCAGGCGCGGCTGCGGATAGTTATGCAGCTTTTTCCAGTCCGGCTTGCCCTGGAACAAGTCGCCCTCCCACCAGGTGGGGCCCGCATCAATCGCTTCTTTCTCA‑GTGCGCGACATCGGCGGCATCACCTTACGGAAACCGCGAAATAcc                                                                                                                                                                  <  1:214882/234‑1 (MQ=255)                                                   ccAGCTCATGGGTGATCTGGAAATCATTCGCCATCCGGCAGGCTTCTTCTACCGGGCCGTCGAGAAACGCTTGCTCTTCGGCGGTCAGGCGCGGCTGCGGATAGTTATGCAGCTTTTTCCAGTCCGGCTTGCCCTGGAACAAGTCGCCCTCCCACCAGGTGGGGCCCGCATCAATCGCTTCTTTCTCA‑GTGCGCGACATCGGCGGCATCACCTTACGGCAACCGCGCAATACCGgc                                                                                                                                                               >  1:762160/1‑236 (MQ=255)                                                    cAGCTCATGGTTGATCTGGAAATCATTCGCCATCCGGCAGGCTTCTTCTACCGGGCCGTCGAGAAACGCTTGCTCTTCGGCGGTCAGGCGCGGCTGCGGATAGTTATGCAGCTTTTTCCAGTCCGGCTTGCCCTGGAACAAGTCGCCCTCCCACCAGGTGGGGCCCGCATCAATCGCTTCTTTCTCA‑GTGCGCGACATCGGCGGCATCACCTTACGGAAACCGCGAAATACCGgcg                                                                                                                                                              <  1:638366/236‑1 (MQ=255)                                                    cAGCTCATGGGTGATCTGGAAATCATTCGCCATCCGGCAGGCTTCTTCTACCGGGCCGTCGAGAAACGCTTGCTCTTCGGCGGTCAGGCGCGGCTGCGGATAGTTATGCAGCTTTTTCCAGTCCGGCTTGCCCTGGAACAAGTCGCCCTCCCACCAGGTGGGGCCCGCATCAATCGCTTCTTTCTCA‑GTGCGCGCCATCGGCGGCATCCCCTTACCGGAACCGCCACATACCGgcg                                                                                                                                                              >  2:214882/1‑236 (MQ=255)                                                             gctcagcTGTAAATGATTCGCATTCCGGCAGGCTTCTGCTCCCGGGCCGTCGAGACACGCTTGCTCTTCGGCGGTCAGGCGCGGCTGCGGATAGTTATGCAGCTTTTTCCAGTCCGGCTTGCCCTGGAACAAGTCGCCCTCCCACCAGGTGGGGCCCGCATCAATCGCTTCTTTCTCA‑GTGCGCGACATCGGCGGCATCACCTTACGGAAACCGCGAAATACCGGCGCGGAAatca                                                                                                                                                     <  2:367683/230‑1 (MQ=255)                                                              gttaTCTGGAAATCATTCGCCATCCGGCAGGCTGCTTCTACCGGGCCGTCGAGAAACGCTTGCTCTTCGGCGGTCAGGCGCGGCTGCGGATAGTTATGCAGCTTTTTCCAGTCCGGCTTGCCCTGGAACAAGTCGCCCTCCCACCAGGTGGGGCCCGCATCAATCGCTTCTTTCTCA‑GTGCGCGACATCGGCGGCATCACCTTACGGAAACCGCGAAATACCGGCGCGGAAttcat                                                                                                                                                    <  1:546662/233‑1 (MQ=255)                                                                  tcgggAATTCGTTCCCCATCGGGCAGGCTTCTGCTGCCGGGCCATCGAGATACGCTTGCTCTGCGGACGTCAGGCGCGGCTGCGGATAGTTATGCAGCTTTTTCCAGTCCGGCTTGCCCTGGAACAAGTCGCCCTCCCACCAGGTGGGGCCCGCATCAATCGCTTCTTTCTCA‑GTGCGCGACATCGGCGGCATCACCTTACGGAAACCGCGAAATACCGGCGCGGAAatcat                                                                                                                                                    <  2:438851/229‑1 (MQ=255)                                                                       aaaTCATTCGCCATCCGGCAGGCTTCTTCTACCGGGCCGTCGAGAAACGCTTGCTCTTCGGCGGTCAGGCGCGGCTGCGGATAGTTATGCAGCTTTTTCCAGTCCGGCTTGCCCTGGAACAAGTCGCCCTCCCACCAGGTGGGGCCCGCATCAATCGCTTCTTTCTCA‑GTGCGCGACATCGGCGGCATCACCTTACGGAAACCGCGAAATACCGGCGCGGAAATCATCGACTTACg                                                                                                                                           >  1:89507/1‑236 (MQ=255)                                                                           cATTCGCCATCCGGCAGGCTTCTTCTACCGGGCCGTCGAGAAACGCTTGCTCTTCGGCGGTCAGGCGCGGCTGCGGATAGTTATGCAGCTTTTTCCAGTCCGGCTTGCCCTGGAACAAGTCGCCCTCCCACCAGGTGGGGCCCGCATCAATCGCTTCTTTTTCA‑GTGCGCGACATCGGCGGCATCACCTTACGGAAACCGCGAAATACCGGCGCCGAAATCATCGACTTatccat                                                                                                                                        >  1:639626/1‑230 (MQ=255)                                                                            aTTCGCCATCCGGCAGGCTTCTTCTACCGGGCCGTCGAGAAACGCTTGCTCTTCGGCGGTCAGGCGCGGCTGCGGATAGTTATGCAGCTTTTTCCAGTCCGGCTTGCCCTGGAACAAGTCGCCCTCCCACCAGGTGGGGCCCGCATCAATCGCTTCTTTCTCA‑GTGCGCGACATCGGCGGCATCACCTTACGGAAACCGCGAAATACCGGCGCGGAAATCATCGACTTa                                                                                                                                             >  1:438851/1‑229 (MQ=255)                                                                                 gcgtcgggCAGGCTTCTCGTGCCGGGCCGTCGAGAACCGCTGGCTGTTGGGCGGTCAGGCGCGGCTGCGGATAGTTATGCAGCTTTTTCCAGTCCGGCTTGCCCTGGAACAAGTCGCCCTCCCACCAGGTGGGGCCGGCATCAATCGCTTCTTTCTCA‑GTGCGCGACATCGGCGGCATCACCTTACGGAAACCGCGAAATACCGGCGCGGAAATCATCGACTTACGCATAGGCGCa                                                                                                                                 <  2:472397/230‑1 (MQ=255)                                                                                     ccGGCAGGCTTCTTCTACCGGGCCGTCGAGAAACGCTTGCTCTTCGGCGGTCAGGCGCGGCTGCGGATAGTTATGCAGCTTTTTCCAGTCCGGCTTGCCCTGGAACAAGTCGCCCTCCCACCAGGTGGGGCCCGCCTCAATCGCTTCTTTCTCA‑GTGCGCGACATCGGCGGCATCACCTTACGGAAACCCCGACAAAACCGCGCCGGAAACATCCGCCTACGCAAAAGCGCCAAgg                                                                                                                             >  2:253851/1‑235 (MQ=255)                                                                                          aGGCTGCTTCTACCGGGCAGTCGAGAAACGCTTGCTCTGCGGCGGTCAGGCGCGGCTGCGGATAGTTATGCAGCTTTGTCCAGTCCGGCTTGCCCTGGAACAAGTCGCCCTCCCACCAGGTGGGGCCCGCATCAATCGCTTCTTTCTCA‑GTGCGCGACATCGGCGGCATCACCTTACGGAAACCGCGAAATACCGGCGCGGAAATCATCGACTTACGCATAGGCGCAAAGTTAAAt                                                                                                                        <  1:332454/236‑1 (MQ=255)                                                                                              ttcctcTACCGGGCCGTCGAGAAACGGTTGCTCTTCGGCGGTCAGGCGCGGCTGCGGATAGTTATGCAGCTTTTTCCAGTCCGGCTTGCCCGGGAACAAGTCGCCCTCCCACCAGGTGGGGCCCGCATCAATCGCTTCTTTCTCA‑GTGCGCGACATCGGCGGCATCACCTTACGGAAACCGCGAAATACCGGCGCGGAAATCATCGACTTACGCATAGGCGCAAAGTTAAATGGCa                                                                                                                    <  2:458218/236‑1 (MQ=255)                                                                                                cttctACCGGGCCGTCGAGAAACGCTTGCTCTTCGGCGGTCAGGCGCGGCTGCGGATAGTTATGCAGCTTTTTCCAGTCCGGCTTGCCCTGGAACAAGTCGCCCTCCCACCAGGTGGGGCCCGCATCAATCGCTTCTTTCTCA‑GTGCGCGACATCGGCGGCATCACCTTACGGAAACCGCGAAATACCGGCGCGGAAATCATCGACTTACGCATAGGCGCAAAGTTAAATGGCAc                                                                                                                   <  1:84449/235‑1 (MQ=255)                                                                                                  tctACCGGGCCGTCGAGAACCGCTTGCTCTGCGGCGGTCAGGCGCGGCTGCGGATAGTTATGCAGCTTTTTCCAGTCCGGCTTGCCCTGGAACAAGTCGCCCTCCCACCAGGTGGGGCCCGCATCAATCGCTTCTTTCTCA‑GTGCGCGACATCGGCGGCATCACCTTACGGAAACCGCGAAATACCGGCGCGGAAATCATCGACTTACGCATAGGCGCAAAGTTAAATGGCACGa                                                                                                                 <  1:632967/235‑1 (MQ=255)                                                                                                      ccGGGCCGTCGAGAAACGCTTGCTCTTCGGCGGTCAGGCGCGGCTGCGGATAGTTATGCAGCTTTTTCCAGTCCGGCTTGCCCTGGAACAAGTCGCCCTCCCACCAGGTGGGGCCCGCATCAATCGCTTCTTTCTCA‑GTGCGCGACATCGGCGGCATCCCCTTACGGAAACCGCGAAATACCGGCGCGGGAATCATCGACTTTCGCCTAAGGGCCAAGGTAAATGGCACGCGGaga                                                                                                            >  2:84449/1‑234 (MQ=255)                                                                                                         ggCCGTCGAGAAACGCTTGCTCTTCGGCGGTCAGGCGCGGCTGCGGATAGTTATGCAGCTTTTTCCAGTCCGGCTTGCCCTGGAACAAGTCGCCCTCCCACCAGGTGGGGCCCGCATCAATCGCTTCTTTCTCA‑GTGCGCGACATCGGCGGCATCACCTTACGGAAACCGCGAAATACCGGCGCGGAAATCATCGACTTACGCATAGGCGCAAAGTTAAATGGCACGAGGATAATg                                                                                                         >  1:749209/1‑236 (MQ=255)                                                                                                          gCCGTCGAGAAACGCTTGCTCTTCGGCGGTCAGGCGCGGCTGCGGATAGTTATGCAGCTTTTTCCAGTCCGGCTTGCCCTGGAACAAGTCGCCCTCCCACCAGGTGGGGCCCGCATCAATCGCTTCTTTCTCA‑GTGCGCGACATCGGCGGCATc                                                                                                                                                                                          <  2:539214/154‑1 (MQ=255)                                                                                                          gCCGTCGAGAAACGCTTGCTCTTCGGCGGTCAGGCGCGGCTGCGGATAGTTATGCAGCTTTTTCCAGTCCGGCTTGCCCTGGAACAAGTCGCCCTCCCACCAGGTGGGGCCCGCATCAATCGCTTCTTTCTCA‑GTGCGCGACATCGGCGGCATc                                                                                                                                                                                          >  1:539214/1‑154 (MQ=255)                                                                                                           ccGTCGAGAAACGCTTGCTCTTCGGCGGTCAGGCGCGGCTGCGGATAGTTATGCAGCTTTTTCCAGTCCGGCTTGCCCTGGAACAAGTCGCCCTCCCACCAGGTGGGGCCCGCATCAATCGCTTCTTTCTCA‑GTGCGCGACATCGGCGGCATCACCTTACGGAAACCGCGAAATACCGGCGCGGAAATCATCGACTTACGCATAGGCGCAAAGTTAAATGGCACGAGGATAATGGc                                                                                                       >  1:362568/1‑236 (MQ=255)                                                                                                              tCGAGAACCGCTTGCTCTTCGGCGGTCAGGCGCGGCTGCGGATAGTTATGCAGCTTTTTCCAGTCCGGCTTGCCCTGGAACAAGTCGCCCTCCCACCAGGTGGGGCCCGCATCAATCGCTTCTTTCTCA‑GTGCGCGACATCGGCGGCATCACCTTACGGAAACCGCGAAATACCGGCGCGGAAATCATCGACTTACGCATAGGCGCAAAGTTAAATGGCACGAGGATAATGGCCa                                                                                                     <  1:743278/235‑1 (MQ=255)                                                                                                                  gaAGCGCTTGCTCTTCGGCGGTCAGGCGCGGCTGCGGATAGTTATGCAGCTTTTTCCAGTCCGGCTTGCCCTGGAACAAGTCGCCCTCCCACCAGGTGGGGCCCGCATCAATCGCTTCTTTCTCA‑GTGCGCGACATCGGCGGCATCACCTTACGGAAACCGCGAAATACCGGCGCGGAAATCATCGACTTACGCATAGGCGCAAAGTTAAATGGCACGAGGATAATGGCCAGAgg                                                                                                 <  1:10151/235‑1 (MQ=255)                                                                                                                           gCTCTTCGGCGGTCAGGCGCGGCTGCGGATAGTTATGCAGCTTTTTCCAGTCCGGCTTGCCCTGGAACAAGTCGCCCTCCCACCAGGTGGGGCCCGCATCAATCGCTTCTTTCTCA‑GTGCGCGACATCGGCGGCATCACCTTACGGAAACCGCGAAATACCGGCGCGGAAATCATCGACTTACGCATAGGCGCAAAGTTAAATGGCACGAGGATAATGGCCAGAGGCACCAGTAc                                                                                        >  1:730493/1‑235 (MQ=255)                                                                                                                                   gctgTCAGGCGCGGCTGCGGATAGTTATGCAGCTTTTTCCAGTCCGGCTTGCCCTGGAACAAGTCGCCCT‑CCACCAGGTGGGGCCCGCATCAATCGCTTCTTTCTCA‑GTGCGCGACATCGGCGGCATCACCTTACGGAAACCGCGAAATACCGGCGCGGAAATCATCGACTTACGCATAGGCGCAAAGTTAAATGGCACGAGGATAATGGCCAGAGGCACCAGTACCCACGCCGAc                                                                              <  1:207790/233‑1 (MQ=255)                                                                                                                                       tCAGGCGCGGCTCCGGATAGTTATGCCGCTTTTTCCGGTCCTGCTTGCCCTGGAACAAGTCGCCCTCCCACCAGGTGGGGCCCGCATCAATCGCTTCTTTCTCA‑GTGCGCGACATCGGCGGCATCACCTTACGGAAACCGCGAAATACCGGCGCGGAAATCATCGACTTACGCATAGGCGCAAAGTTAAATGGCACGAGGATAATGGCCAGAGGCACCAGTACCCACGCCGACca                                                                            <  2:468255/235‑1 (MQ=255)                                                                                                                                       tCAGGCCCGGCTGCGGATGATAATGCAGCTTTTTCCAGTCCGGCTTGCCCTGGAACAAGTCGCCCTCCCACCAGGTGGGGCCCGCATCAATCGCTTCTTTCTCA‑GTGCGCGACATCGGCGGCATCACCTTACGGAAACCGCGAAATACCGGCGCGGAAATCATCGACTTACGCATAGGCGCAAAGTTAAATGGCACGAGGATAATGGCCAGAGGCACCAGTACCCACGCCGACca                                                                            <  2:218811/235‑1 (MQ=255)                                                                                                                                                gCTGCGGATAGTTATGCAGCTTTTTCCAGTCCGGCTTGCCCTGGAACAAGTCGCCCTCCCACCAGGTGGGGCCCGCATCAATCGCTTCTTTCTCA‑GTGCGCGACATCGGCGGCATCACCTTACGGAAACCGCGAAATACCGGCGCGGAAATCATCGACTTACGCATAGGCGCAAAGTTAAATGGCACGAGGATAATGGCCAGAGGCACCAGTACCCACGCCGACCACAGACCAGCa                                                                  <  2:749209/236‑1 (MQ=255)                                                                                                                                                gCTGCGGATAGTTATGCAGCTTTTTCCAGTCCGGATTGCCCTGGAACAAGTCGCCCTCCCACCAGGTGGGGCCCGCATCAATCGCTTCTTTCTCA‑GTGCGCGACATCGGCGGCATCACCTTACGGAAACCGCGAAATACCGGCGCGGAAATCATCGACTTACGCATAGGCGCAAAGTTAAATGGCACGAGGATAATGGCCAGAGGCACCAGTACCCACGCCGACCACAGACCAGc                                                                   <  2:89507/235‑1 (MQ=255)                                                                                                                                                                  gttttacccAGTCCGGGTTGCCTTGGAACAAGTCGCCCTCCCACCAGGGGGGGCCCGCATCAATCGCTTCTTTCTCA‑GGGCGCGACATCGGCGGCATCACCTTACGGAAACCGCGAAATACCGGCGCGGAAATCATCGACTTACGCATAGGCGCAAAGTTAAATGGCACGAGGATAATGGCCAGAGGCACCAGTACCCACGCCGACCACAGACCAGCAACGCCGAGGGCGGCTGTc                                                <  2:356664/229‑1 (MQ=255)                                                                                                                                                                     ttttCCAGTCCGGCTTGCCCTGGAACAAGTCGCCCTCCCACCAGGTGGGGCCCGCATCAATCGCTTCTTTCTCA‑GTGCGCGACATCGGCGGCATCACCTTACGGAAACCGCGAAATACCGGCGCGGAAATCATCGACTTACGCATAGGCGCCAAGTTAAATGGCACGAGGATAATGGCCAGAGGCACCAGGAACCACGCCGACCACAGACCAGCAACGCCCAGGGCGGCTGTACat                                             >  2:508257/1‑235 (MQ=255)                                                                                                                                                                     ttttCCAGTCCGGCTTGCCCTGGAACAAGTCGCCCTCCCACCAGGTGGGGCCCGCATCAATCGCTTCTTTCTCA‑GTGCGCGACATCGGCGGCATCACCTTACGGAAACCGCGAAATACCGGCGCGGAAATCATCGACTTACGCATAGGCGCACAGTTAAATGGCACGAGGATAATGGCCAGAGGCACCAGTACCCACGCCGACCACAGACCAGCAAAGCCGAGGGCGGCTGTCCAg                                             >  1:470885/1‑236 (MQ=255)                                                                                                                                                                     ttttCCAGTCCGGCTTGCCCTGGAACAAGTCGCCCTCCCACCAGGTGGGGCCCGCATCAATCGCTTCTTTCTCA‑GTGCGCGACATCGGCGGCATCACCTTACGGAAACCGCGAAATACCGGCGCGGAAATCATCGACTTACGCATAGGCGCAAAGTTAAATGGCACGa                                                                                                                 >  2:543150/1‑168 (MQ=255)                                                                                                                                                                     ttttCCAGTCCGGCTTGCCCTGGAACAAGTCGCCCTCCCACCAGGTGGGGCCCGCATCAATCGCTTCTTTCTCA‑GTGCGCGACATCGGCGGCATCACCTTACGGAAACCGCGAAATACCGGCGCGGAAATCATCGACTTACGCATAGGCGCAAAGTTAAATGGCACGa                                                                                                                 <  1:543150/168‑1 (MQ=255)                                                                                                                                                                     ttttCCAGTCCGGCTTGCCCTGGAACAAGTCGCCCTCCCACCAGGTGGGGCCCGCATCAATCGCTTCTTTCTCA‑GTGCGCGACATCGGCGGCATCACCTTACGGAAACCGCGAAATACCGGCGCGGAAATCATCGACTTACGCATAGGCGCAAAGTTAAATGGCACGAGGATAATGGCCAGAGGCACCAGTACCCACGCCGACCACAGACCAGCAACGCCGAGGGCGGCTGTCCAg                                             >  1:472112/1‑236 (MQ=255)                                                                                                                                                                     ttttCCAGTCCGGCTTGCCCTGGAACAAGTCGCCCTCCCACCAGGTGGGGCCCGCATCAATCGCTTCTTTCTCA‑GTGCGCGACATCGGCGGCATCACCTTACGGAAACCGCGAAATACCGGCGCGGAAATCATCGACTTACGCATAGGCGCAAAGTTAAATGGCACGAGGATAATGGCCAGAGGCACCAGTACCCACGCCGACCACAAACCAGCAACGCCGACGGAGGCTggtccg                                             >  2:749524/1‑231 (MQ=255)                                                                                                                                                                     ttttCCAGTCCGGCTTGCCCTGGAACAAGTCGCCCTCCACCCAGGTGGGGCCCGCATCAA‑CCCCTCCTTCTCAGGTCCCCACCATCGCCGGCACACCCTTCCGGAACCCCCGAAAACCCGCCCGGGAAATCATCGACTTACGCATAGGCGCAAGGTTAAATGGCCCAGGGATAATGGCCAGAGGCCCAGGTACCCACGCCGCCAACAGCCAAGCAACGCCGAGGGCGGCTGGCCac                                             >  2:204717/1‑235 (MQ=255)                                                                                                                                                                          cAGTCGGGCTTGCCCTGGAACCAGTCTCCCTCCCACCAGGTGGGGCCCGCATCAATCGCTTCTTTCTCA‑GTGCGCGACAGCGGCGGCATCCCCTTACGGAAACCGCGAAATACCGGCGCGGAAATCATCGACTTACGCATAGGCGCAAAGTTAAATGGCACGAGGATAATGGCCAGAGGCACCAGTACCCACGCCGACCACAGACCAGCAACGCCGAGGGCGGCTGTCCAGGCGAg                                        <  2:30381/236‑1 (MQ=255)                                                                                                                                                                          cAGTCCGGCTTGCCCTGGAACAAGTCGCCCTCCCACCAGGTGGGGCCCGCATCAATCGCTTCTTTCTCA‑GTGCGCGACATCGGCGGCATCACCTTACGGAAACCGCGAAATACCGGCGCGGAAATCATCGACTTACGCATAGGCGCAAAGTTAAATGGCACGAGGATAATGGCCAGAGGCACCAGTACCCACGCCGACCACAGACCAGCAACGCCGAGGGCGGCTGTCCAGGCGAg                                        <  2:730493/236‑1 (MQ=255)                                                                                                                                                                          cAGTCCGGCTTGCCCTGGAACAAGTCGCACTCCCACCAGGTGGGGCCCGCATCAATCGCTTCTGTCTCA‑GTGCGCGACATCGGCGGCATCACCTTACGGAAACCGCGAAATACCGGCGCGGAAATCATCGACTTACGCATAGGCGCAAAGTTAAATGGCACGAGGATAATGGCCAGAGGCACCAGTACCCACGCCGACCACAGACCAGCAACGCCGAGGGCGGCTGTCCAGGCGAg                                        <  1:334189/236‑1 (MQ=255)                                                                                                                                                                             tCCGGCTTGCCCTGGAACAAGTCGCCCTCCCACCAGGTGGGGCCCGCATCAATCGTTTCTTTCTCA‑GTGCGCGACATCGGCGGCATCACCTTACGGAAACCGCGAAATACCGGCGCGGAAATCATCGACTTACGCATAGGCGCAAAGTTAAATGGCACGAGGATAATGGCCAGAGGCACCAGTACCCACGCCGACCACAGACCAGCAACGCCGAg                                                          >  2:712972/1‑215 (MQ=255)                                                                                                                                                                             tCCGGCTTGCCCTGGAACAAGTCGCCCTCCCACCAGGTGGGGCCCGCATCAATCGCTTCTTTCTCA‑GTGCGCGACATCGGCGGCATCACCTTACGGAAACCGCGAAATACCGGCGCGGAAATCATCGACTTACGCATAGGCGCAAAGTTAAATGGCACGAGGATAATGGCCCGAGGCACCAGTAACCACGCCGACCACAGACCAGCAACGCCGAGGGCGGCTGTCCAGGCGAGCaa                                     >  2:81605/1‑236 (MQ=255)                                                                                                                                                                             tCCGGCTTGCCCTGGAACAAGTCGCCCTCCCACCAGGTGGGGCCCGCATCAATCGCTTCTTTCTCA‑GTGCGCGACATCGGCGGCATCACCTTACGGAAACCGCGAAATACCGGCGCGGAAATCATCGACTTACGCATAGGCGCAAAGTTAAATGGCACGAGGATAATGGCCAGAGGCACCAGTACCCACGCCGACCACAGACCAGCAACGCCGAg                                                          <  1:712972/215‑1 (MQ=255)                                                                                                                                                                                  cTTGCCCTGGAACAAGTCGCCCTCCCACCAGGTGGGGCCCGCATCAATCGCTTCTTTCTCA‑GTGCGCGACATCGGCGGCATCACCTTACGGAAACCGCGAAATACCGGCGCGGAAATCATCGACTTACGCATAGGCGCAAAGTTAAATGGCACGAGGATAATGGCCAGAGGCACCAGTACCCACGCCGACCACAGACCAGCAACGCCGAGGGCGGCTGTCCAGGCGAGCAAAATCa                                <  1:159622/236‑1 (MQ=255)                                                                                                                                                                                    tGCCCTGGAACAAGTCGCCCTCCCACCAGGTGGGGCCCGCATCAATCGCTTCTTTCTCA‑GTGCGCGACATCGGCGGCATCACCTTACGGAAACCGCGAAATACCGGCGCGGAAATCATCGACTTACGCATAGGCGCAAAGTTAAATGGCACGAGGATAATGGCCAGAGGCACCAGTACCCACGCCGACCACAGACCAGCAACGCCGAGGGCGGCTGTCCAGGCGAGCAAAATCAGa                              <  1:218527/236‑1 (MQ=255)                                                                                                                                                                                      cccTGGAACAATTGCGCCTCCCACCAGGTGCGACCCGCATCAATCGCTTCTTTCTCA‑GTGCGCGACATCGGAGGCATCACCTTACGGAAACCGCGAAATACCGGCGCGGAAATCATCGACTTACGCATAGGCGCAAAGTTAAATGGCACGAGGATAATGGCCAGAGGCACCAGTACCCACGCCGACCACAGACCAGCAACGCCGAGGGCGGCTGTCCAGGCGAGCAAAATCAGAc                             <  2:639626/235‑1 (MQ=255)                                                                                                                                                                                        cTGGAACAAGTCGCCCTCCCACCAGGTGGGGCCCGCATCAATCGCTTCTTTCTCA‑GTGCGCGACATCGGCGGCATCACCTTACGGAAACCGCGAAATACCGGCGCGGAAATCATCGACTTACGCATAGGCGCAAAGTTAAATGGCACGAGGATAATGGCCAGAGGCACCAGTACCCACGCCGACCACAGACCAGCAACGCCGAGGGCGGCTGTCCAGGCGAGCAAAATCAGActgc                          <  2:362568/236‑1 (MQ=255)                                                                                                                                                                                          ggAACAAGTCGCCCTCCCACCAGGTGGGGCCCGCATCAATCGCTTCTTTCTCA‑GTGCGCGACATCGGCGGCATCACCTTACGGAAACCGCGAAATACCGGCGCGGAAATCATCGACTTACGCATAGGCGCAAAGTTAAATGGCACGAGGATAATGGCCAGAGGCACCAGTACCCACGCCGACCACAGACCAGCAACGCCGAGGGCGGCTGTCCAGGCGAGCAAAATCAGActgctg                        <  1:393793/236‑1 (MQ=255)                                                                                                                                                                                                aGTCGCCCTCCCACCAGGTGGGGCCCGCATCAATCGCTTCTTTCTCA‑GTGCGCGACATCGGCGGCATCACCTTACGGAAACCGCGAAATACCGGCGCGGAAATCATCGACTTACGCATAGGCGCAAAGTTAAATGGCACGAGGATAATGGCCAGAGGCACCAGGACCCACCCCGACCACAGACCAGCAACGCCGAGGGCGGCTTGCCAGGCGAGCCACATCAGaacgcggtgaaa                   >  2:393793/1‑224 (MQ=255)                                                                                                                                                                                                      ccTCCCACCAGGTGGGGCCCGCATCAATCGCTTCTTTCTCA‑GTGCGCGACATCGGCGGCATCACCTTACGGAAACCGCGAAATACCGGCGCGGAAATCATCGACTTACGCATAGGCGCAAAGTTAAATGGCACGAGGATAATGGCCAGAGGCACCAGTACCCACGCCGACCACAGACCAGCAACGCCGAGGGCGGCTGTCCAGGCGAGCAAAATCAGAct                            <  1:382445/220‑1 (MQ=255)                                                                                                                                                                                                      ccTCCCACCAGGTGGGGCCCGCATCAATCGCTTCTTTCTCA‑GTGCGCGACATCGGCGGCATCACCTTACGGAAACCGCGAAATACCGGCGCGGAAATCATCGACTTACGCATAGGCGCAAAGTTAAATGGCACGAGGATAATGGCCAGAGGCACCAGTACCCACGCCGACCACAGACCAGCAACGCCGAGGGCGGCTGTCCAGGCGAGCAAAATCAGAct                            >  2:382445/1‑220 (MQ=255)                                                                                                                                                                                                      ccTCCCACCAGGTGGGGCCCGCATCAATCGCTTCTTTCTCA‑GTGCGCGACATCGGCGGCATCACCTTACGGAAACCGCGAAATACCGGCGCGGAAATCATCGACTTACGCATAGGCGCAAAGTTAAATGGCACGAGGATAATGGCCAGAGGCACCAGTACCCACGCCGACCACAGACCAGCAACGCCGAGGGCGGCTGTCCAGGCGAGCAAAATCAGACTGCTGATAAATAAGCt             <  1:293432/235‑1 (MQ=255)                                                                                                                                                                                                       cTCCCACCAGGTGGGGCCCGCATCAATCGCTTCTTTCTCA‑GTGCGCGACATCGGCGGCATCACCTTACGGAAACCGCGAAATACCGGCGCGGAAATCATCGACTTACGCATAGGCGCAAAGTTAAATGGCACGAGGATAATGGCCAGAGGCACCAGTACCCACGCCGACCACAGACCAGCAACGCCGAGGGCGGCTGTCCAGGCGAGCAAAATCAGACTGCTGATAAATAAGCTCa           <  1:419965/236‑1 (MQ=255)                                                                                                                                                                                                          cgcccaGGTCGGGCCCGCATCAATCGCTTCTTTCTCA‑ATGCGCGACATCGGCGGCATCACCTTACGGAAACCGCGAAATACCGGCGCGGAAATCATCGACTTACGCATAGGCGCAAAGTTAAATGGCACGAGGATAATGGCCAGAGGCACCAGTACCCACGCCGACCACAGACCAGCAACGCCGAGGGCGGCTGTCCAGGCGAGCAAAATCAGACTGCTGATAAATAAGCTCAcgc        <  2:470885/233‑1 (MQ=255)                                                                                                                                                                                                          ccaccaGGTGGGGCCCGCATCAATCGCTTCTTTCTCA‑GTGCGCGACATCGGCGGCATCACCTTACGGAAACCGCGAAATACCGGCGCGGAAATCATCGACTTACGCATAGGCGCAAAGTTAAATGGCACGAGGATAATGGCCAGAGGCACCAGTACCCACGCCGACCACAGACCAGCAACGCCGAGGGCGGCTGTCCAGGCGAGCAAAATCAGACTGCTGATAAATAAGCTCAcgc        <  1:253851/236‑1 (MQ=255)                                                                                                                                                                                                                ggtgggGCCCGCATCAATCGCTTCTTTCTCA‑GTGCGCGACATCGGCGGCATCACCTTACGGAAACCGCGAAATACCGGCGCGGAAATCATCGACTTACGCATAGGCGCAAAGTTAAATGGCACGAGGATAATGGCCAGAGGCACCAGTACCCACGCCGACCACAGACCAGCAACGCCGAGGGCGGCCGGCCCGGCGAGCAAAATCAGACTGCTGATAAATAAGCTCACGCGGTGAt  >  2:16988/1‑236 (MQ=255)                                                                                                                                                                                                                     |                                                                                                                                                                                                                                        GAAGAAACGATGCTCTTTAAGGTACGCCCACAACTCCGGCGGCAGATCCGCCAGCTCATGGGTGATCTGGAAATCATTCGCCATCCGGCAGGCTTCTTCTACCGGGCCGTCGAGAAACGCTTGCTCTTCGGCGGTCAGGCGCGGCTGCGGATAGTTATGCAGCTTTTTCCAGTCCGGCTTGCCCTGGAACAAGTCGCCCTCCCACCAGGTGGTGCCCGCATCAATCGCTTCTTTCTCA‑GTGCGCGACATCGGCGGCATCACCTTACGGAAACCGCGAAATACCGGCGCGGAAATCATCGACTTACGCATAGGCGCAAAGTTAAATGGCACGAGGATAATGGCCAGAGGCACCAGTACCCACGCCGACCACAGACCAGCAACGCCGAGGGCGGCTGTCCAGGCGAGCAAAATCAGACTGCTGATAAATAAGCTCACGCGGTGAT  >  NC_000913/242809‑243251

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑