Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,325,833	T→G	V363G (GTC→GGC)	yciQ →	enhancer of membrane protein expression; putative inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,325,833	0	T	G	98.3%	184.2 / NA	58	V363G (GTC→GGC)	yciQ	enhancer of membrane protein expression; putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base G (31/26);  total (31/27)
Fisher's exact test for biased strand distribution p-value = 4.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGGCATGTTACGTCTCGATGCGAAGCTAGTTTACGACGATAAAGGTTTTTGTGCCGATATCGTAAATCTGATTGTGAAAGGAAAAATTCATCTGGAAGATCAGTCTGACAAGAACCAGCAAATCCTGATTCGTGTTAATGAAGGCGCGACCAGAAATAATGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCA‑TTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGAGTCTGGTGGGCGATATGTTTATTATGATCTGCTGGATTATTCCTTTT  >  NC_000913/1325612‑1326062                                                                                                                                                                                                                              |                                                                                                                                                                                                                                       cGGCATGTTACGTCTCGATGCGAAGCTAGTTTACGACGATAAAGGTTTTTGTGCCGATATCGTAAATCTGATTGTGAAAGGAAAAATTCATCTGGAAGATCAGTCTGACAAGAACCAGCAAATCCTGATTCGTGTTAATGAAGGCGCGACCAGAAATAATGCGGTATTACTGCCCTCAGAGCGTGTATTACAGGAAGCGTTATTTCGTAAAGGCCATAATGGCGTCCTTActgcga                                                                                                                                                                                                                          >  2:297780/1‑231 (MQ=255)              ctcGATGCGAAGCTAGTTTACGACGATAAAGGTTTTTGTGCCGATATCGTAAATCTGATTGTGAAAGGAAAAATTCATCTGGAAGATCAGTCTGACAAGAACCAGCAAATCCTGATTCGTGTTAATGAAGGCGCGACCAGAAATAATGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGCTAAGGGCGTTCTTACGGGGAGACGCAACagag                                                                                                                                                                                                              >  1:446889/1‑235 (MQ=255)              ctcGATGCGAAGCTAGTTTACGACGATAAAGGTTTTTGTGCCGATATCGTAAATCTGATTGTGAAAGGAAAAATTCATCTGGAAGATCAGTCTGACAAGAACCAGCAAATCCTGATTCGTGTTAATGAAGGCGCGACCAGAAATAATGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGGCGTTCTTACGGGGAGACGCAACAGAGt                                                                                                                                                                                                             >  1:474865/1‑236 (MQ=255)                 gATGCGAAGCTAGTTTACGACGATAAAGGTTTTTGTGCCGATATCGTAAATCTGATTGTGAAAGGAAAAATTCATCTGGAAGATCAGTCTGACAAGAACCAGCAAATCCTGATTCGTGTTAATGAAGGCGCGACCAGAAATAATGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGGCGTTCTTACGGGGAGACGCAACAGAGTCtt                                                                                                                                                                                                          >  1:118115/1‑236 (MQ=255)                  aTGCGAAGCTAGTTTACGACGATAAAGGTTTTTGTGCCGATATCGTAAATCTGATTGTGAAAGGAAAAATTCATCTGGAAGATCAGTCTGACAAGAACCAGCAAATCCTGATTCGTGTTAATGAAGGCGCGACCAGAAATAATGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGGCGGTCTTACGTGGAGACGCAAAAGAGTCTTa                                                                                                                                                                                                         >  2:723446/1‑236 (MQ=255)                    gCGAAGCTAGTTTACGACGATAAAGGTTTTTGTGCTGATATCGTAAATCTGATTGTGAAAGGAAAAATTCATCTGGAAGATCAGTCTGACAAGAACCAGCAAATCCTGATTCGTGTTAATGAAGGCGCGACCAGAAATAATGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGGCGTTCTTACGGGGAGACGCAACAGAGTCTTACg                                                                                                                                                                                                       <  2:118115/236‑1 (MQ=255)                                  cgacgaTAAAGGTTTTTGTGCCGATATCGTAAATCTGATTGTGAAAGGAAAAATTCATCTGGAAGATCAGTCTGACAAGAACCAGCAAATCCTGATTCGTGTTAATGAAGGCGCGACCAGAAATAATGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGTGATAAAGGCGTTCTTACGGGGAGACGCACCAGAGACTTACGCAGGGCATTTTTAc                                                                                                                                                                                         >  2:685781/1‑236 (MQ=255)                                   gacgaTAAAGGTTTTTGTGCCGATATCGTAAATCTGATTGTGAAAGGAAAAATTCATCTGGAAGATCAGTCTGACAAGAACCAGCAAATCCTGATTCGTGTTAATGAAGGCGCGACCAGAAATAATGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGGCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACg                                                                                                                                                                                        >  2:588276/1‑236 (MQ=255)                                         aaaGGTATGTGTGCCGATATCGTAAATCTGATTGTGAAAGGAAAAATTCATCTGGAAGATCAGTCTGACAAGAACCAGCAAATCCTGATTCGTGTTAATGAAGGCGCGACCAGAAATAATGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGGCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCa                                                                                                                                                                                  <  2:170100/236‑1 (MQ=255)                                                tttGTGCCGATATCGTAAATCTGATTGTGAAAGGAAAAATTCATCTGGAAGATCAGTCTGACAAGAACCAGCAAATCCTGATTCGTGTTAATGAAGGCGCGACCAGAAATAATGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGGCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAAttt                                                                                                                                                                           >  2:133452/1‑236 (MQ=255)                                                tttGTGCCGATATCGTAAATCTGATTGTGAAAGGAAAAATTCATCTGGAAGATCAGTCTGACAAGAACCAGCAAATCCTGATTCGTGTTAATGAAGGCGCGACCAGAAATAATGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGGCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAAttt                                                                                                                                                                           >  1:566910/1‑236 (MQ=255)                                                 ttGTGCCGATATCGTAAATCTGATTGTGAAAGGAAAAATTCATCTGGAAGATCAGTCTGACAAGAACCAGCAAATCCTGATTCGTGTTAATGAAGGCGCGACCAGAAATAATGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGGCGTTCTTACGGGGGGAGGCAACAGAGGCTTACGCAGGGCATTTTTTCGGATGCAGAAAttgt                                                                                                                                                                          >  2:11387/1‑234 (MQ=255)                                                    tgCCGATATCGTAAATCTGATTGTGAAAGGAAAAATTCATCTGGAAGATCAGTCTGACAAGAACCAGCAAATCCTGATTCGTGTTAATGAAGGCGCGACCAGAAATAATGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGAGCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTAt                                                                                                                                                                        >  2:444108/1‑235 (MQ=255)                                                         atatCGTAAATCTGATTGTGAAAGGAAAAATTCATCTGGAAGATCAGTCTGACAAGAACCAGCAAATCCTGATTCGTGTTAATGAAGGCGCGACCAGAAATAATGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGGCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAAtt                                                                                                                                                                            >  1:156853/1‑226 (MQ=255)                                                             cGTAAATCTGATTGTGAAAGGAAAAATTCATCTGGAAGATCAGTCTGACAAGAACCAGCAAATCCTGATTCGTGTTAATGAAGGCGCGACCAGAAATAATGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGGCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTa                                                                                                                                                              >  1:592709/1‑236 (MQ=255)                                                                      gATTGTGAAAGGAAAAATTCATCTGGAAGATCAGTCTGACAAGAACCAGCAAATCCTGATTCGTGTTAATGAAGGCGCGACCAGAAATAATGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGGCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGc                                                                                                                                                                                                 >  1:490268/1‑192 (MQ=255)                                                                      gATTGTGAAAGGAAAAATTCATCTGGAAGATCAGTCTGACAAGAACCAGCAAATCCTGATTCGTGTTAATGAAGGCGCGACCAGAAATAATGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGGCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGc                                                                                                                                                                                                 <  2:490268/192‑1 (MQ=255)                                                                              aaGGAAAAATTCATCTGGAAGATCAGTCGGACAAGAACCAGCAAATCCTGATTCGTGTTAATGAAGGCGCGACCAGAAATAATGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGGCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTAc                                                                                                                                             <  2:585663/236‑1 (MQ=255)                                                                                      aTTCATCTGGAAGATCAGTCTGACAAGAACCAGCAAATCCTGATTCGTGTTAATGAAGGCGCGACCAGAAATAATGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGGCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGa                                                                                                                                     <  1:239895/236‑1 (MQ=255)                                                                                                   aTCAGTCTGACAAGAACCAGCAAATCCTGATTCGTGTTAATGAAGGCGCGACCAGAAATAATGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGGCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCa                                                                                                                         <  1:723446/235‑1 (MQ=255)                                                                                                              aaGAACCAGCAAATCCTGATTCGTGTTAATGAAGGCGCGACCAGAAATAATGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGGCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACt                                                                                                             <  1:23027/236‑1 (MQ=255)                                                                                                                           tCCTGATTCGTGTTAATGAAGGCGCGACCAGAAATAATGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGGCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTgg                                                                                                           <  1:133452/225‑1 (MQ=255)                                                                                                                           tCCTGATTCGTGTTAATGAAGGCGCGACCAGAAATAATGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGGCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCg                                                                                                >  1:581446/1‑236 (MQ=255)                                                                                                                                     tgtTAATGAAGGCGCGACCAGAAATAATGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGGCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGTGGTCC‑TTCttt                                                                                     >  1:263087/1‑236 (MQ=255)                                                                                                                                     tgtTAATGAAGGCGCGACCAGAAATAATGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGGCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCggactatactt                                                                                      >  2:720792/1‑227 (MQ=255)                                                                                                                                               ggCGCGACCAGAAATAATGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGGCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGCTACTTTTTTGCAATGGGGTTGACTGGCAATATTGGCGGGTG‑TTCTTTACgcgtaagt                                                                           >  2:336400/1‑229 (MQ=255)                                                                                                                                               ggCGCGACCAGAAATAATGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGGCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCACTGGGGTGGACTGGCAATATTGGCGGGGA‑TTCctttttgtcactg                                                                           >  2:62214/1‑226 (MQ=255)                                                                                                                                               ggCGCGACCAGAAATAATGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGGCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCC‑TTCCTTACGGtgaatt                                                                           >  1:718685/1‑231 (MQ=255)                                                                                                                                                 ccggACCAGAAATAATCCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGGCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCA‑TTCTTTACGGTAACTTGa                                                                         <  2:199676/233‑1 (MQ=255)                                                                                                                                                    gACCAGAAATAATGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGGCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATa                                                                                                                                   <  2:738157/176‑1 (MQ=255)                                                                                                                                                    gACCAGAAATAATGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGGCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATa                                                                                                                                   >  1:738157/1‑176 (MQ=255)                                                                                                                                                                tGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGGCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCACTGGGGTGGACTGGCAATATTGGCGGTCA‑TTTTTTTCTGGAAATTGGGGTCTGTTGGCTggg                                                          >  2:274893/1‑236 (MQ=255)                                                                                                                                                                tGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGGCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCA‑TTCTTTTCGGTCACTTGAGTCCTGtcagcttgg                                                          >  2:223786/1‑228 (MQ=255)                                                                                                                                                                 gCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGGCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCA‑TTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGc                                                         <  2:566910/236‑1 (MQ=255)                                                                                                                                                                 gCGGTATTACTGCCCGCAGAGCAGTTATTACGGGAAGCGTTATTTCGTAAAGGCGATAAGGGCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCA‑TTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGc                                                         <  1:431502/236‑1 (MQ=255)                                                                                                                                                                    gTATTACTGCCCGCAGTGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCTATAAGGGCGTTCTTACTGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCA‑TTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAgg                                                      <  1:720792/236‑1 (MQ=255)                                                                                                                                                                        tACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGGCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCA‑TTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGc                                                         <  1:79186/229‑1 (MQ=255)                                                                                                                                                                           tGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGGCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCA‑TTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGAGt                                               <  1:11387/236‑1 (MQ=255)                                                                                                                                                                               tgCAGAGCAGTGAGGACGGGAAGCGTTATTTCGTAAAGGCGATAAGGGCGTTCTGACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATCCTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCA‑TTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGAGTCtgg                                           <  2:446889/235‑1 (MQ=255)                                                                                                                                                                               cGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGGCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTTA‑TTCTTTTCGGTAAATTGAGTCCCGTAGGCTTGGCTGGGATggtgctcg                                           >  2:281339/1‑229 (MQ=255)                                                                                                                                                                               cGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGGCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCT‑TTCTTTTCGGTTACTTTAGTCCTGTTGGCTgggatggggttggt                                               >  2:79186/1‑221 (MQ=255)                                                                                                                                                                               cGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGGCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTTTTTTAGGGTAATTAGGGTCCTTTGGACGGGGCGGGactaggctag                                            >  2:492919/1‑227 (MQ=255)                                                                                                                                                                                 cAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGGCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCA‑TTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGAGTCtggtg                                         <  2:156853/236‑1 (MQ=255)                                                                                                                                                                                  agagCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGGCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCA‑TTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGAGTCtggtgg                                        <  1:588276/236‑1 (MQ=255)                                                                                                                                                                                      cAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGGCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCA‑TTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGAGTCTGGTGGGCGa                                    <  1:492919/236‑1 (MQ=255)                                                                                                                                                                                        gtgtgtaCTGGAAGCTTTATTTCGTATAGGCGATAAGTGCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCA‑TTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGAGTCTGGTGGGCGat                                   <  2:718685/230‑1 (MQ=255)                                                                                                                                                                                           attaCTGGAAGCGTTATGTGGTAAAGGCGTTAAGGGCGTTCTTACGGGTAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCA‑TTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGAGTCTGGTGGGCGATATg                                <  2:654380/235‑1 (MQ=255)                                                                                                                                                                                              aCTGGAAGCGTTATTTCGTAAAGGCGATAAGGGCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTgg                                                                                                                >  1:666812/1‑153 (MQ=255)                                                                                                                                                                                              aCTGGAAGCGTTATTTCGTAAAGGCGATAAGGGCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTgg                                                                                                                <  2:666812/153‑1 (MQ=255)                                                                                                                                                                                                   aatcGTAATTTCGTAAAGGTGATAAGGTCGTTCTAACGAGAAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCA‑TTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGAGTCTGGTGGGCGATATGTTTATTATg                       <  2:114284/233‑1 (MQ=255)                                                                                                                                                                                                   aaGCGTTATTTCGTAAAGGCGATAAGGGCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGc                                                                                                                                                                    <  2:139785/96‑1 (MQ=255)                                                                                                                                                                                                   aaGCGTTATTTCGTAAAGGCGATAAGGGCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGc                                                                                                                                                                    >  1:139785/1‑96 (MQ=255)                                                                                                                                                                                                   aaGCGTTATTTCGTAAAGGCGATAAGGGCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCA‑TTCTTTACTGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGAGTCTGGTGGGTGGTATGTttatta                         >  1:363402/1‑234 (MQ=255)                                                                                                                                                                                                   aaGCGTTATTTCGTAAAGGCGATAAGGGCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCA‑TTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGAGTCTGGTGGGCGATATGTTTATTATg                       <  1:274893/236‑1 (MQ=255)                                                                                                                                                                                                   aaGCGTTAGTGCGTAAAGGTGAATAGGGCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCA‑TTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGAGTCTGGTGGGCGATATGTTTATTATg                       <  2:49605/236‑1 (MQ=255)                                                                                                                                                                                                         tATTTCGTAAAGGCGATAAGGGCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCA‑TTCTTTACGGTAACTTGAGTCCt                                                                    <  1:547990/185‑1 (MQ=255)                                                                                                                                                                                                                        aTAAGGGCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCA‑TTCTTTACGGTAACTTTAGTTCTGTAGGCTGGGGCGGGATGAGTCTGGTTGGCGGTATGGTTATTATGGTCTGTTGGGTGATTTCtttt  >  1:765896/1‑236 (MQ=255)                                                                                                                                                                                                                        aTAAGGGCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCA‑TTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGAGTCTGGTGGGCGATATGTTTATTATGATCTGCTGGGTTATTCCtttt  >  1:442996/1‑236 (MQ=255)                                                                                                                                                                                                                              |                                                                                                                                                                                                                                       CGGCATGTTACGTCTCGATGCGAAGCTAGTTTACGACGATAAAGGTTTTTGTGCCGATATCGTAAATCTGATTGTGAAAGGAAAAATTCATCTGGAAGATCAGTCTGACAAGAACCAGCAAATCCTGATTCGTGTTAATGAAGGCGCGACCAGAAATAATGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCA‑TTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGAGTCTGGTGGGCGATATGTTTATTATGATCTGCTGGATTATTCCTTTT  >  NC_000913/1325612‑1326062

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑