Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,753,535	Δ1 bp	intergenic (+79/‑70)	recN → / → bamE	recombination and repair protein/lipoprotein component of BamABCDE OM biogenesis complex

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,753,533	0	A	.	100.0%	185.2 / NA	42	intergenic (+77/‑72)	recN/bamE	recombination and repair protein/lipoprotein component of BamABCDE OM biogenesis complex
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (18/24);  total (18/24)

TGGTGCGATGACAGAAACGCATATGCAATCCCTGAATAAAAAAGCGCGGTTACAAGAGCTGGCGCGCCTGCTTGGTGGCAGTGAAGTCACACGTAATACACTGGCGAATGCGAAAGAACTGCTTGCAGCGTAAACTTTTTTCCTGCTTCACGGTCAGAGTAAACAGCAAAACGCCGTAAGACCGGAAAGCAAAAGGTTTTAAAGTGATGAAAGGTCTATTATCATCGGCATATTACAGATGAGCCACGTACTGCTCGGGCCCGAAAAGGAATCAAATCACTATGCGCTGTAAAACGCTGACTGCTGCAGCAGCAGTACTATTGATGTTGACCGCAGGCTGTTCCACTCTGGAGCGAGTGGTTTACCGTCCTGACATCAACCAGGGGAACTATCTGACCGCTAACGACGTATCCAAAATACGTG  >  NC_000913/2753324‑2753746                                                                                                                                                                                                                  |                                                                                                                                                                                                                      tGGTGCGATGACAGAAACGCATATGCAATCCCTGAATAAAAAAGCGCGGTTACAAGAGCTGGCGCGCCTGCTTGGTGGCAGTGAAGTCACACGTAATACACTGGCGAATGCGAAAGAACTGCTTGCAGCGTAAACTTTTTTCCTGCTTCACGGTCAGAGTAAACAGCAAAACGCCGTAAGACCGGAAAGCAAAAGGTTTTAAAGTGATG‑AAGGTCTATTATCATCg                                                                                                                                                                                                      <  1:154423/226‑1 (MQ=255)           aCAGAAACGCATATGCAATCCCTGAATAAAAAAGCGCGGTTACAAGAGCTGGCGCGCCTGCTTGGTGGCAGTGAAGTCACACGTAATACACTGGCGAATGCGAAAGAACTGCTTGCAGCGTAAACTTTTTTCCTGCTTCACGGTCAGAGTAAACAGCAAAACGCCGTAAGACCGGAAAGCAAAAGGTTTTAAAGTGATG‑AAGGTCTATTATCATCGGCATATTACAg                                                                                                                                                                                           >  2:553579/1‑227 (MQ=255)            cAGAAACGCATATGCAATCCCTGAATAAAAAAGCGCGGTTACAAGAGCTGGCGCGCCTGCTTGGTGGCAGTGAAGTCACACGTAATACACTGGCGAATGCGAAAGAACTGCTTGCAGCGTAAACTTTTTTCCTGCTTCACGGTCAGAGTAAACAGCAAAACGCCGTAAGACCGGAAAGCAAAAGGTTTTAAAGTGATG‑AAGGTCTATTATCATCGGCATATTACAGAt                                                                                                                                                                                         <  2:365751/228‑1 (MQ=255)                  cGCATATGCAATCCCTGAATAAAAAAGCGCGGTTACAAGAGCTGGCGCGCCTGCTTGGTGGCAGTGAAGTCACACGTAATACACTGGCGAATGCGAAAGAACTGCTTGCAGCGTAAACTTTTTTCCTGCTTCACGGTCAGAGTAAACAGCAAAACGCCGTAAGACCGGAAAGCAAAAGGTTTTAAAGTGATG‑AAGGTCTATTATCATCGGCATATTACAGATGAGcc                                                                                                                                                                                    <  1:361679/227‑1 (MQ=255)                               ccTGAATAAAAAAGCGCGGTTACAAGAGCTGGCGCGCCTGCTTGGTGGCAGTGAAGTCACACGTAATACACTGGCGAATGCGAAAGAACTGCTTGCAGCGTAAACTTTTTTCCTGCTTCACGGTCAGAGTAAACAGCAAAACGCCGTAAGACCGGAAAGCAAAAGGTTTTAAAGTGATG‑AAGGTCTATTATCATCGGCATATTACAGATGAGCCACGTACTGCTCggg                                                                                                                                                                      >  1:521518/1‑228 (MQ=255)                                 tGAATAAAAAAGCGCGGTTACAAGAGCTGGCGCGCCTGCTTGGTGGCAGTGAAGTCACACGTAATACACTGGCGAATGCGAAAGAACTGCTTGCAGCGTAAACTTTTTTCCTGCTTCACGGTCAGAGTAAACAGCAAAACGCCGTAAGACCGGAAAGCAAAAGGTTTTAAAGTGATG‑AAGGTCTATTATCATCGGCATATTACAGATGAGCCACGTACTGCTCGGGcc                                                                                                                                                                    >  1:507317/1‑228 (MQ=255)                                      aaaaaaGCGCGGTTACAAGAGCTGGCGCGCCTGCTTGGTGGCAGTGAAGTCACACGTAATACACTGGCGAATGCGAAAGAACTGCTTGCAGCGTAAACTTTTTTCCTGCTTCACGGTCAGAGTAAACAGCAAAACGCCGTAAGACCGGAAAGCAAAAGGTTTTAAAGTGATG‑AAGGTCTATTATCATCGGCATATTACAGATGAGCCACGTACTGCTCGGGCCCGaaa                                                                                                                                                               <  2:506496/228‑1 (MQ=255)                                                           cTGGCGCGCCTGCTTGGTGGCAGTGAAGTCACACGTAATACACTGGCGAATGCGAAAGAACTGCTTGCAGCGTAAACTTTTTTCCTGCTTCACGGTCAGAGTAAACAGCAAAACGCCGTAAGACCGGAAAGCAAAAGGTTTTAAAGTGATG‑AAGGTCTATTATCATCGGCATATTACAGATGAGCCACGTACTGCTCGGGCCCGAAAAGGAATCAAATCACTATgcg                                                                                                                                           <  1:403683/227‑1 (MQ=255)                                                            tGGCGCGCCTGCTTGGTGGCAGTGAAGTCACACGTAATACACTGGCGAATGCGAAAGAACTGCTTGCAGCGTAAACTTTTTTCCTGCTTCACGGTCAGAGTAAACAGCAAAACGCCGTAAGACCGGAAAGCAAAAGGTTTTAAAGTGATG‑AAGGTCTATTATCATCGGc                                                                                                                                                                                                    >  2:139579/1‑169 (MQ=255)                                                            tGGCGCGCCTGCTTGGTGGCAGTGAAGTCACACGTAATACACTGGCGAATGCGAAAGAACTGCTTGCAGCGTAAACTTTTTTCCTGCTTCACGGTCAGAGTAAACAGCAAAACGCCGTAAGACCGGAAAGCAAAAGGTTTTAAAGTGATG‑AAGGTCTATTATCATCGGCATATTACAGATGAGCCACGTACTGCTCGGGCCCGAAAAGGAATCAAATCACTATg                                                                                                                                             >  1:472935/1‑224 (MQ=255)                                                             ggCGCGCCTGCTTGGTGGCAGTGAAGTCACACGTAATACACTGGCGAATGCGAAAGAACTGCTTGCAGCGTAAACTTTTTTCCTGCTTCACGGTCAGAGTAAACAGCAAAACGCCGTAAGACCGGAAAGCAAAAGGTTTTAAAGTGATG‑AAGGTCTATTATCATCGGCa                                                                                                                                                                                                   <  1:139783/169‑1 (MQ=255)                                                              gcgcgcCTGCTTGGTGGCAGTGAAGTCACACGTAATACACTGGCGAATGCGAAAGAACTGCTTGCAGCGTGAACTTTTTTCCTGCTTCACGGTCAGAGTAAACAGCAAAACGCCGTAAGACCGGAAAGCAAAAGGTTTTAAAGTGATG‑AAGGTCTATTATCATCGGCATATTACAGATGAGCCACGTACTGATCGGGCCCGAAAAGGAATCAAATACATATGCGCAGt                                                                                                                                       >  2:558837/1‑228 (MQ=255)                                                              gcgcgcCTGCTTGGTGGCAGTGAAGTCACACGTAATACACTGGCGAATGCGAAAGAACTGCTTGCAGCGTAAACTTTTTTCCTGCTTCACGGTCAGAGTAAACAGCAAAACGCCGTAAGACCGGAAAGCAAAAGGTTTTAAAGTGATG‑AAGGTCTATTATCATCGGCATATTACAGATGAGCCACGTACTGCTCGGGCCCGAAAAGGAATCAAATCACTATg                                                                                                                                             >  1:234681/1‑222 (MQ=255)                                                                   ccTGCTTGGTGGCAGTGAAGTCACACGTAATACACTGGCGAATGCGAAAGAACTGCTTGCAGCGTAAACTTTTTTCCTGCTTCACGGTCAGAGTAAACAGCAAAACGCCGTAAGACCGGAAAGCAAAAGGTTTTAAAGTGATG‑AAGGTCTATTATCATCGGCATATTACAGATGAGCCACGTACTGCTCGGGCCCGAAAAGGa                                                                                                                                                           >  1:21798/1‑203 (MQ=255)                                                                    cTGCTTGGTGGCAGTGAAGTCACACGTAATACACTGGCGAATGCGAAAGAACTGCTTGCAGCGTAAACTTTTTTCCTGCTTCACGGTCAGAGTAAACAGCAAAACGCCGTAAGACCGGAAAGCAAAAGGTTTCAAAGTGATG‑AAGGTCTATTATCATCGGCATATTACAGATGAGCCACGTACTGCTCGGGCCCGAAAAGGaa                                                                                                                                                          <  2:21762/203‑1 (MQ=255)                                                                       cTTGGTGGCAGTGAAGTCACACGTAATACACTGGCGAATGCGAAAGAACTGCTTGCAGCGTAAACTTTTTTCCTGCTTCACGGTCAGAGTAAACAGCAAAACGCCGTAAGACCGGAAAGCAAAAGGTTTTAAAGTGATG‑AAGGTCTATTATCATCGGCATATTACAGATGAGCCACGTACTGCTCGGGCCCGAAAAGGAATCAAATCACTATGCGCTGTAAAACGCt                                                                                                                               <  2:98608/227‑1 (MQ=255)                                                                        ttGGTGGCAGTGAAGTCACACGTAATACACTGGCGAATGAGAAAGAACTGCTTGCAGCGTAAACTTTTTTCCTGCTTCACGGTCAGAGTAAACAGCAAAACGCCGTAAGACCGGAAAGCAAAAGGTTTTAAAGTGATG‑AAGGTCTATTATCATCGGCATATTACAGATGAGCCACGTACTGCTCGGGCCCGAAAAGGAATCAAATCACTATGCGCTGTAAAACGCTg                                                                                                                              <  1:101599/227‑1 (MQ=255)                                                                                   gAAGTCACACGTAATACACTGGCGAATGCGAAAGAACTGCTTGCAGCGTAAACTTTTTTCCTGCTTCACGGTCAGAGTAAACAGCAAAACGCCGTAAGACCGGAAAGCAAAAGGTTTTAAAGTGATG‑AAGGTCTATTATCATCGGCATATTACAGATGAGCCACGTACTGCTCGGGCCCGAAAAGGAATCAAATCACTATGCGCTGTAAAACGCTGACTGCTgcagc                                                                                                                   <  1:510464/227‑1 (MQ=255)                                                                                              tAATACACTGGCGAATGCGAAAGAACTGCTTGCAGCGTAAACTTTTTTCCTGCTTCACGGTCAGAGTAAACAGCAAAACGCCGTAAGACCGGAAAGCAAAAGGTTTTAAAGTGATG‑AAGGTCTATTATCATCGGCATATTACAGATGAGCCACGTACTGCTCGGGCCCGAAAAGGAATCAAATCACTATGCGCTGTAAAACGCTGACTGCTGcagcagcag                                                                                                              <  1:554467/221‑1 (MQ=255)                                                                                                        gCGAATGCGAAAGAACTGCTTGCAGCGTAAACTTTTTTCCTGCTTCACGGTCAGAGTAAACAGCAAAACGCCGTAAGACCGGAAAGCAAAAGGTTTTAAAGTGATG‑AAGGTCt                                                                                                                                                                                                                >  2:67485/1‑113 (MQ=255)                                                                                                        gCGAATGCGAAAGAACTGCTTGCAGCGTAAACTTTTTTCCTGCTTCACGGTCAGAGTAAACAGCAAAACGCCGTAAGACCGGAAAGCAAAAGGTTTTAAAGTGATG‑AAGGTCTATTATCATCGGCATATTACAGATGAGCCACGTACTGCTCGGGCCCGAAAAGGAATCAAATCACTATGCGCTGTAAAACGCTGACTGCTGCAGCAGCAGTACTATTGATGTTGAc                                                                                              >  2:534019/1‑227 (MQ=255)                                                                                                         cGAATGCGAAAGAACTGCTTGCAGCGTAAACTTTTTTCCTGCTTCACGGTCAGAGTAAACAGCAAAACGCCGTAAGACCGGAAAGCAAAAGGTTTTAAAGTGATG‑AAGGTCta                                                                                                                                                                                                               <  1:67583/113‑1 (MQ=255)                                                                                                             tGCGAAAGAACTGCTTGCAGCGTAACCTTTTTTCCTGCTTCACGGTCAGAGTAAACAGCAAAACGCCGTAAGACCGGAAAGCAAAAGGTTTTAAAGTGATG‑AAGGTCTATTATCATCGGCATATTACAGATGAGCCACGTACTGCTCGGGCCCGAAAAGGAATCAAATCACTATGCGCTGTAAAACGCTGACTGCTGCAGCAGCAGTACTATTGATGTTGACCGCAgg                                                                                        <  1:295093/228‑1 (MQ=255)                                                                                                                          cTTGCAGCGTAAACTTTTTTCCTGCTTCACGGTCAGAGTAAACAGCAAAACGCCGTAAGACCGGAAAGCAAAAGGTTTTAAAGTGATG‑AAGGTCTATTATCATCGGCATATTACAGATGAGCCACGTACTGCTCGGGCCCGAAAAGGAATCAAATCACTATGCGCTGTAAAAc                                                                                                                                  >  1:588683/1‑173 (MQ=255)                                                                                                                           ttGCAGCGTAAACTTTTTTCCTGCTTCACGGTCAGAGTAAACAGCAAAACGCCGTAAGACCGGAAAGCAAAAGGTTTTAAAGTGATG‑AAGGTCTATTATCATCGGCATATTACAGATGAGCCACGTACTGCTCGGGCCCGAAAAGGAATCAAATCACTATGCGCTGTAAAACg                                                                                                                                 <  2:587743/173‑1 (MQ=255)                                                                                                                                           tttCCTGCTTCACGGTCAGAGTAAACAGCAAAACGCCGTAAGACCGGAAAGCAAAAGGTTTTAAAGTGATG‑AAGGTCTATTATCATCGGCATATTACAGa                                                                                                                                                                                          >  1:28905/1‑100 (MQ=255)                                                                                                                                            ttCCTGCTTCACGGTCAGAGTAAACAGCAAAACGCCGTAAGACCGGAAAGCAAAAGGTTTTAAAGTGATG‑AAGGTCTATTATCATCGGCATATTACAGAt                                                                                                                                                                                         <  2:28861/100‑1 (MQ=255)                                                                                                                                               cTGCTTCACGGTCAGAGTAAACAGCAAAACGCCGTAAGACCGGAAAGCAAAAGGTTTTAAAGTGATG‑AAGGTCTATTATCATCGGCATATTACAGATGAGCCACGTACTGCTCGGGCCCGAAAAGGAATCAAATCACTATGCGCTGTAAAACGCTGACTGCTGCAGCAGCAGTACTATTGATGTTGACCGCAGGCTGTTCCACTCTGGAGCGAGTGGTTTACCGTcc                                                       <  2:555048/227‑1 (MQ=255)                                                                                                                                                 gCTTCACGGTCAGAGTAAACAGCAAAACGCCGTAAGACCGGAAAGCAAAAGGTTTTAAAGTGATG‑AAGGTCTATTATCATCGGCATATTACAGATGAGCCACGTACTGCTCGGGCCCGAAAAGGAATCAAATCACTATGCGCTGTAAAACGCTGACTGCTGCAGCAGCAGTACTATTGATGTTGACCGCAGGCTGTTCCACTCTGGAGCGAGTGGTTTACCGTCCTGa                                                    <  1:534883/228‑1 (MQ=255)                                                                                                                                                  cTTCACGGTCAGAGTAAACAGCAAAACGCCGTAAGACCGGAAAGCAAAAGGTTTTAAAGTGATG‑AAGGTCTATTATCATCg                                                                                                                                                                                                      >  2:681488/1‑81 (MQ=255)                                                                                                                                                   ttCACGGTCAGAGTAAACAGCAAAACGCCGTAAGACCGGAAAGCAAAAGGTTTTAAAGTGATG‑AAGGTCTATTATCATCgg                                                                                                                                                                                                     <  1:682604/81‑1 (MQ=255)                                                                                                                                                                   aCAGCAAAACGCCGTAAGACCGGAAAGCAAAAGGTTTTAAAGTGATG‑AAGGTCTATTATCATCGGCATATTACAGATGAGCCACGTACTGCTCGGGCCCGAAAAGGAATCAAATCACTATGCGCTGTAAAACGCTGACTGCTGCAGCAGCAGTACTATTGATGTTGACCGCAGGCTGTTCCACTCTGGAGCGAGTGGTTTACCGTCCTGACATCAACCAGGGGAACTa                                  >  2:605585/1‑228 (MQ=255)                                                                                                                                                                        aaaaCGCCGTAAGACCGGAAAGCAAAAGGTTTTAAAGTGATGaa                                                                                                                                                                                                                      >  1:576662/1‑44 (MQ=255)                                                                                                                                                                        aaaaCGCCGTAAGACCGGAAAGCAAAAGGTTTTAAAGTGATG‑AAGGTCTATTATCATCGGCATATTACAGATGAGCCACGTACTGCTCGGGCCCGAAAAGGAATCAAATCACTATGCGCTGTAAAACGCTGActg                                                                                                                          >  2:568608/1‑135 (MQ=255)                                                                                                                                                                        aaaaCGCCGTAAGACCGGAAAGCAAAAGGTTTTAAAGTGATG‑AAGGTCTATTATCATCGGCATATTACAGATGAGCCACGTACTGCTCGGGCCCGAAAAGGAATCAAATCACTATGCGCTGTAAAACGCTGACTGCTGCAGCAGCAGTACTATTGATGTTGACCGCAGGCt                                                                                      >  1:454248/1‑171 (MQ=255)                                                                                                                                                                         aaaCGCCGTAAGACCGGAAAGCAAAAGGTTTTAAAGTGATGaag                                                                                                                                                                                                                     <  2:575741/44‑2 (MQ=255)                                                                                                                                                                         aaaCGCCGTAAGACCGGAAAGCAAAAGGTTTTAAAGTGATG‑AAGGTCTATTATCATCGGCATATTACAGATGAGCCACGTACTGCTCGGGCCCGAAAAGGAATCAAATCACTATGCGCTGTAAAACGCTGActgc                                                                                                                         <  1:569521/135‑1 (MQ=255)                                                                                                                                                                         aaaCGCCGTAAGACCGGAAAGCAAAAGGTTTTAAAGTGATG‑AAGGTCTATTATCATCGGCATATTACAGATGAGCCACGTACTGCTCGGGCCCGAAAAGGAATCAAATCACTATGCGCTGTAAAACGCTGACTGCTGCAGCAGCAGTACTATTGATGTTGACCGCAGGCTg                                                                                     <  2:453505/171‑1 (MQ=255)                                                                                                                                                                          aaCGCCGTAAGACCGGAAAGCAAAAGGTTTTAAAGTGATG‑AAGGTCTATTATCATCGGCATATTACAGATGAGCCACGTACTGCTCGGGCCCGAAAAGGAATCAAATCACTATGCGCTGTAAAACGCTGACTGCTGCAGCAGCAGTACTATTGATGTTGACCGCAGGCTGTTCCACTCTGGAGCGAGTGGTTTACCGTCCTGACATCAACCAGGGGAACTATCt                               <  1:606556/224‑1 (MQ=255)                                                                                                                                                                            cGCCGTAAGACCGGAAAGCAAAAGGTTTTAAAGTGATG‑AAGGTCTATTATCATCGGc                                                                                                                                                                                                    >  1:496955/1‑57 (MQ=255)                                                                                                                                                                             gCCGTAAGACCGGAAAGCAAAAGGTTTTAAAGTGATG‑AAGGTCTATTATCATCGGCa                                                                                                                                                                                                   <  2:496148/57‑1 (MQ=255)                                                                                                                                                                                         gAAAGCAAAAGGTTTTAAAGTGATG‑AAGGTCTATTATCATCGGCATATTACAGATGAGCCACGTACTGCTCGGGCCCGAAAAGGAATCAAATCACTATGCGCTGTAAAACGc                                                                                                                                >  1:152384/1‑112 (MQ=255)                                                                                                                                                                                          aaaGCAAAAGGTTTTAAAGTGATG‑AAGGTCTATTATCATCGGCATATTACAGATGAGCCACGTACTGCTCGGGCCCGAAAAGGAATCAAATCACTATGCGCTGTAAAACGCt                                                                                                                               <  2:152164/112‑1 (MQ=255)                                                                                                                                                                                                   ggTTTTAAAGTGATG‑AAGGTCTATTATCATCGGCATATTACAGATGAGCCACGTACTGCTCGGGCCCGAAAAGGAATCAAATCACTATGCGCTGTAAAACGCTGACTGCTGCAGCAGCAGTACTATTGATGTTGACCGCAGGCTGTTCCACTCTGGAGCGAGTGGTTTACCGTCCTGACATCAACCAGGGGAACTATCTGACCGCTAACGACGTATCCAAAATACgtg  <  1:559734/228‑1 (MQ=255)                                                                                                                                                                                                                  |                                                                                                                                                                                                                      TGGTGCGATGACAGAAACGCATATGCAATCCCTGAATAAAAAAGCGCGGTTACAAGAGCTGGCGCGCCTGCTTGGTGGCAGTGAAGTCACACGTAATACACTGGCGAATGCGAAAGAACTGCTTGCAGCGTAAACTTTTTTCCTGCTTCACGGTCAGAGTAAACAGCAAAACGCCGTAAGACCGGAAAGCAAAAGGTTTTAAAGTGATGAAAGGTCTATTATCATCGGCATATTACAGATGAGCCACGTACTGCTCGGGCCCGAAAAGGAATCAAATCACTATGCGCTGTAAAACGCTGACTGCTGCAGCAGCAGTACTATTGATGTTGACCGCAGGCTGTTCCACTCTGGAGCGAGTGGTTTACCGTCCTGACATCAACCAGGGGAACTATCTGACCGCTAACGACGTATCCAAAATACGTG  >  NC_000913/2753324‑2753746

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑