Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,825,130	0	G	T	22.9%	143.8 / 11.3	70	intergenic (‑193/+10)	ves/spy	cold‑ and stress‑inducible protein/periplasmic ATP‑independent protein refolding chaperone, stress‑induced
Reads supporting (aligned to +/- strand):  ref base G (26/28);  new base T (16/0);  total (42/28)
Fisher's exact test for biased strand distribution p-value = 8.17e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

ATTCACCGACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGG‑TCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTG‑CAGGCATTTTACCTTTTGCCGCTGGACGTTCT‑G‑TCAGACGCTT‑CTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCT‑GTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGC  >  NC_000913/1824902‑1825365                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                 aTTCACCGACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCTTTACTGCAAGGTAGTGGACAAGACCGGCGG‑TCTTATGTTTTTTTGCTgcattag                                                                                                                                                                                                                                        >  2:299953/1‑233 (MQ=255)   tccccGACATTTTACGCATATCAAGGTATTCCATACTCGCTCCCGGCCCGCTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGGGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGGAAACCGCCGAACCTGTACACCGCCTTACTTCAAGGTAGTGGACAAGACCGGCGG‑GCTTACGTATTTTTGCTGTAAGaata                                                                                                                                                                                                                                      >  2:2017958/4‑239 (MQ=255)                                tCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGG‑TCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTG‑CAGGCAttttt                                                                                                                                                                                                               >  1:1854406/1‑231 (MQ=255)                                tCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGG‑TCTTAAGGTTTTTTGCTGAAAGATTATTCAGCCGTTT‑CAGGCATTTTTCCtttt                                                                                                                                                                                                         >  1:2024150/1‑238 (MQ=255)                                tCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGG‑TCTTAAGGTTTTTGGCTGAAAGATTATTCAGCAGGTG‑CAGGCATTTTa                                                                                                                                                                                                               >  1:132474/1‑232 (MQ=255)                                  cATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGG‑TCTTAAGTTTTTTTGCTGAAAGATTATTTAGCAGTTG‑c                                                                                                                                                                                                                         >  1:111405/1‑220 (MQ=255)                                   ttccTCGCCCCCGTCCCTTTTTTTTCATTATTGTGGTGCAGTGAGTCGTGGATTGTTTTCGGCAACGGGGGTTATTTACTTTAGTGGGGCTGGGGTTTTTTTAACAATTTTGGGAGAAGAAAAAAAAAACCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGG‑TCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTG‑CAGGCATTTTACCTTTTGCCg                                                                                                                                                                                                     <  2:132474/236‑1 (MQ=255)                                    tACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGG‑TCTTATGGTTTTTGGTGGAAAGATTATTTAGTAGTTG‑CCGGCATTTTATCTTTTGcc                                                                                                                                                                                                      >  1:694240/1‑237 (MQ=255)                                                 cccGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGG‑TCTTAAGGTTTTTGGCTGAAAGATTATTTAGCAGTTT‑GCGGCATTTTACCCTTTTCCGCCGGACGtt                                                                                                                                                                                            >  1:780120/1‑234 (MQ=255)                                                                  tATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGG‑TCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTG‑CAGGCCTTTTACCTTTTGCCGCTGGACGTTCT‑G‑TCAGACGCTT‑CTCAAAAtt                                                                                                                                                                   >  1:1382150/1‑239 (MQ=255)                                                                                      aTGGTTATCGGCAACTCGGGTGATTTACGTTAGGGGCGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGCTCTT‑AGTTTTTTGGGTCAAAAATTATGTAGCCGTTGCCGGGCTTTTTACC‑TTTCTCGCTGGAC‑TTCTCG‑TAAGAAGCTT‑CTCAAAATATGCCTTAAAtt                                                                                                                                                        >  2:1513445/1‑230 (MQ=255)                                                                                          ttATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGG‑TCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTG‑CAGGCATTTTACCTTTTGCCGCTgg                                                                                                                                                                                                 >  1:1182505/1‑188 (MQ=255)                                                                                                    cTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGG‑TCTTAAGGTTTTTTGCTGAAAGGTTTTTCAGCAGTTG‑CAGGCCTTTTTCCTTTTTCCGCTGGGCGTTCT‑GTTCAGCCG‑TT‑CTCAAAAATAGCATTTAATTTCGTTTTTTGTTCCGGCGtacgg                                                                                                                                 >  2:286541/1‑235 (MQ=255)                                                                                                    cTCGGGGGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACAGGCGG‑TCTTAACGTTTTTGGCTGAAAAATTATTCAGCACATG‑CGGGCATTTTTCCTTTTCCCGGCGGCGGGTCT‑T‑TCAGACGCTTCCTCAAATTTA‑CTTTAAAATTCTTTTCTTGTCTCGGCGTCTgg                                                                                                                                 >  2:201814/1‑239 (MQ=255)                                                                                                             tttACGTTGGGGGTAACCAGCTTTTTTTTAAAACTGTTGGAAAAGAAAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGG‑TCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTG‑CAGGCATTTTACCTTTTGCCGCTGGACGTTCT‑G‑TCAGACGCTt‑ctc                                                                                                                                                                         <  1:635249/190‑1 (MQ=255)                                                                                                                   ttAGTGGTTACCAGTTTTTTTTTACACTTGTGTGAGAAGAAAAAAAAAACCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGG‑TCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTG‑CAGGCATTTTACCTTTTGCCGCTGGACGTTCT‑G‑TCAGACGCTT‑CTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTg                                                                                                                  <  1:286541/239‑1 (MQ=255)                                                                                                                       tggtgATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGG‑TCTTAAGTTTTTTGGCTGAAAGATTATTTAAAAGTTG‑CAGGCATTTTACCTTTTGCCGCTGGACGTTCT‑G‑t                                                                                                                                                                                      >  1:1856605/1‑168 (MQ=255)                                                                                                                       tggtgATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGG‑CCTTAAGTTTTTTTGCTGGAAGATTTTTCAGCAGATG‑CAGGCATTTTATCTTTTGTCGCCGGACGTTCT‑G‑TTAGACGCTT‑CTCAAAATTATCa                                                                                                                                                               >  2:635249/1‑190 (MQ=255)                                                                                                                            aTCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGG‑TCTTAAGTTTTTTTGCTGAAAGATTATTCAGCAGTTG‑GAGGCATTTTACCTTTTGCCGCTgg                                                                                                                                                                                                 >  1:1937555/1‑154 (MQ=255)                                                                                                                                    ttttttACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGG‑TCTTAAGTTTTTTTGCTGAAAGATTATTCAGCAGTTG‑CAGGCATTTTACCTTTTGCCGCTGGACGTTCT‑T‑Tc                                                                                                                                                                                     >  1:527949/1‑156 (MQ=255)                                                                                                                                         actaatgttttGAAAAAAAAAAACAAACCCGCCGATCCTGTCCACCGCATTACTGCTAGGTAGTGGACAAGACCGGCGG‑TCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTG‑CAGGCATTTTACCTTTTGCCGCTGGACGTTCT‑G‑TCAGACGCTT‑CTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCa                                                                                            <  2:1182505/231‑1 (MQ=255)                                                                                                                                                 gttGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGG‑TCTTAAGTTTTTTGGCTGAAAAATTATTTTCCAGTTG‑CAGGCGTTTTTCCTTTTGCCGGTGGACGTTCT‑T‑TTAGACGCCT‑CTCCAAATTAGAAATAAATTTCCTTGTTTGTTTCGGCGCCAGGGTGTTTTCAATTTTGTTCTCGGTTTCTATATGCGTCAGAATGTTa                                                                                    >  2:1819328/2‑239 (MQ=255)                                                                                                                                                           gaaaagaaaaCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGG‑TCTTCAGTGTTTTTGCCGAAAGATTATTCTGCAGTTG‑CATGCATTTTACCTTTTGCCGCTGGGCGTTCT‑G‑TCAGACGCGT‑TTCAAAATTATCATTTTAGTGCTTTTTCTTTTTCGTCGTTCGGATGTTGTAAATTTTGTTCTGGGGTTTCATTTGCGGCAGCATGTTAGCttttc                                                                             >  2:1844461/1‑234 (MQ=255)                                                                                                                                                                    aCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGG‑TCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTG‑CAGGCATTTTACCTTTTTCCGCTGGACGTTCT‑G‑TCAGACGCTT‑CTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTCGCGCT‑GTTCTTCCa                                                                >  2:802180/1‑239 (MQ=255)                                                                                                                                                                            tCCTGTCCGCCGCATTTCTGCAAGGTAGTGGACAAGACCGGCGG‑TCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTG‑Ca                                                                                                                                                                                                                        <  1:788349/83‑1 (MQ=255)                                                                                                                                                                                 tCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGG‑TCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTG‑CAGGCATTTTACCTTTTGCCGCTGGACGTTCT‑G‑TCAGACGCTT‑CTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGtt                                                                                                                <  1:243597/179‑1 (MQ=255)                                                                                                                                                                                      cGCATTACTGCAAGGTAGTGGACAAGACCGGCGG‑TCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTG‑CAGGCATTTTACCTTTTGCCGCTGGACGTTCT‑G‑TCAGACGCTT‑CTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCT‑GTTCTTCCATTTTTGCGATCTGCGCtt                                              <  2:1946911/239‑1 (MQ=255)                                                                                                                                                                                      cGCATTACTGCAAGGTAGTGGACAAGACCGGCGG‑TCTTAAGTTTTTTGGCTGAAAGAGTTTTCAGCAGTTG‑CAGGCCTTTTAACTTTTTCCGCCgg                                                                                                                                                                                                 >  1:1503003/1‑96 (MQ=255)                                                                                                                                                                                        cATTACTGCAAGGTAGTGGACAAGACCGGCGG‑TCTTAAGTTTTTTGGc                                                                                                                                                                                                                                                <  1:188352/48‑1 (MQ=255)                                                                                                                                                                                        cATTACTGCAAGGTAGTGGACAAGACCGGCGG‑TCTTAAGTTTTTTGGc                                                                                                                                                                                                                                                >  2:188352/1‑48 (MQ=255)                                                                                                                                                                                         aTTACTGCAAGGTAGTGGACAAGACCGGCGG‑TCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTG‑CAGGCATTTTACCTTTTGCCGCTGGACGTTCT‑G‑TCAGACGCTT‑CTCa                                                                                                                                                                        <  1:361460/115‑1 (MQ=255)                                                                                                                                                                                         aTTACTGCAAGGTAGTGGACAAGACCGGCGG‑TCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTG‑CAGGCATTTTACCTTTTGCCGCTGGACGTTCT‑G‑TCAGACGCTT‑CTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAg                                                                                           <  1:553763/192‑1 (MQ=255)                                                                                                                                                                                           tACTGCAAGGTAGTGGACAAGACCGGCGG‑TCTTAAGTTTTTTTGCTGAAAGATTATTTAGCAGTTG‑CAGGCCTTTTACCTTTTGCCGCTGGACGTTCT‑G‑TCAGACGCTT‑CTCAAAATTAGCATTAAATTTCTTTTTTTGTTTCCGCCTCAGGGTGTTTTAAATTTTGTTCTGGGttttc                                                                                                      >  2:243597/1‑177 (MQ=255)                                                                                                                                                                                           tACTGCAAGGTAGTGGACAAGACCGGCGG‑TCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTG‑CAGGCATTTTACCTTTTGCCGCTGGACGTTCT‑G‑TCAGACGCTT‑CTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGtt                                                                                                                <  1:1120965/169‑1 (MQ=255)                                                                                                                                                                                               gCAAGGTAGTGGACAAGACCGGCGG‑TCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTG‑CAGGCATTTTACCTTTTTCCGCTGGACGTTCT‑G‑TCAGACGCTT‑CTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCT‑GTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTa                                     <  1:1751619/239‑1 (MQ=255)                                                                                                                                                                                                cAAGGTAGTGGACAAGACCGGCGG‑TCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTG‑CAGGCATTTTACCTTTTGCCGCTGGACGTTCT‑G‑TCAGACGCTT‑CTCAAAAt                                                                                                                                                                    >  1:764329/1‑112 (MQ=255)                                                                                                                                                                                                cAAGGTAGTGGACAAGACCGGCGG‑TCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTG‑CAGGCATTTTACCTTTTGCCGCTGGACGTTCT‑G‑TCAGACGCTT‑CTCAAAAt                                                                                                                                                                    <  2:764329/112‑1 (MQ=255)                                                                                                                                                                                                 aaGGTAGTGGACAAGACCGGCGG‑TCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTG‑CAGGCATTTTACCTTTTGCCGCTGGACGTTCT‑G‑TCAGACGCTT‑CTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTCGCGCT‑GTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACt                                   <  1:802180/239‑1 (MQ=255)                                                                                                                                                                                                   ggTAGTGGACAAGACCGGCGG‑TCTTAAGTTTTTTTGCTGAAAGATTATTCAGCAGTTG‑CAGGGATTTTACCTTTTTGCGCTgg                                                                                                                                                                                                 >  2:361460/1‑83 (MQ=255)                                                                                                                                                                                                   ggTAGTGGACAAGACCGGCGG‑TCTTAAGGTTTTTTGGTGAAAGATTATTCAGCAGTTG‑CAGGCCTTTTACCTTTTTCCGCTgg                                                                                                                                                                                                 >  2:553763/1‑83 (MQ=255)                                                                                                                                                                                                     tAGTGGACAAGACCGGCGG‑TCTTAAGGTTTTTTGGTGAAAGAATATTTAGCAGTTG‑CCGGGATTTTTCCTTTTTCCGCCGGGCGTTCT‑G‑TCAGACGCTT‑CTCAAAAATAACCTTTAAATGCTTTTTTTTTTTTGGCGGCAGGATGGTGTAAATTTTGTTTTGGGttttc                                                                                                      >  2:1120965/1‑167 (MQ=255)                                                                                                                                                                                                        tGGACAAGACCGGCGG‑TCTTAAGTTTTTTGGCTGAAAGATTATTTAGCAGTTG‑Ca                                                                                                                                                                                                                        >  1:764133/1‑55 (MQ=255)                                                                                                                                                                                                        tGGACAAGACCGGCGG‑TCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTG‑Ca                                                                                                                                                                                                                        <  2:764133/55‑1 (MQ=255)                                                                                                                                                                                                         ggACAAGACCGGCGG‑TCTTAAGTTTTGTGGTTGAAAGATTATTCAGCAGTTG‑CAGGCATTTTACCTTTTGCCGCTGGGCGTTCT‑G‑TCAGACGCTT‑CTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCT‑GTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGa                            <  1:388910/238‑1 (MQ=255)                                                                                                                                                                                                          gACAAGACCGGCGG‑TCTTAAGTTTTTTGGCTGAAAGATTATTCAGCCGTTG‑CAGGCATTTTACCTTTTTCCGCTGGGCGTTCT‑G‑TCAGACGCTT‑CTCAAAATTAGCATTAAATTGCTTTTTCTGTTTCGGCGGCAGGATGTTGTAAATTTTTTTTTGGGTTTTCCTTTGCGCCAGCATGTTTGCTTTTCGCT‑GTTCTTTCCTTTTTTTGATCTGCGCTTCTGCCTTTTCTTTATCGAAg                          >  2:1470406/1‑239 (MQ=255)                                                                                                                                                                                                             aaGACCGGCGG‑TCTTAAGTTTTTTTGCTGAAAGATTATTCAGCAATTG‑CCGGCCTTTTACCTTTTTCCGCTGGACGGTCT‑T‑TCCGACGCTT‑TTCCAAATTTGCATTACATTTCTTTTTCTGTTTCGGCCTCAGGGTTGTGTCAATTTTTTTTTTGGTTTTCATGTGTGCCACGCTGTGTGCTTTTCGCTCTTTCTTCCTTTTTTTC‑ATTTGCGCTGTAGCTTTTACTTTTTcttgtgta                       >  2:2127326/1‑232 (MQ=255)                                                                                                                                                                                                               gACCGGCGG‑TCTTAAGTTTTTTTGCTGAAAGCTTATTTAGCAATTG‑CCGGCCCTTTTCCTTTTTTCGCTGGCCGTTCT‑G‑TCAGACCCCT‑CCCAAAATTATCTCTTAAATGCCTTTTCATTTCCCGCGTCCGGATGTTTCAAAATTTTTTCTGTGTTTGCATGTTCTCCACCATGTTATCTTTGCGTG‑GTTTTTCCATTTTTGCGCTCTCCGGCTCTGTGTTTTCTTTGTCTAAgggagt                     >  2:1342021/1‑235 (MQ=255)                                                                                                                                                                                                               gACCGGCGG‑TCTTAAGTTTTTTTGCTGAAAGATTTTTCAGCAGGTT‑CAGGGATTTTACCTTTTTTCGCTGGACGTTCT‑T‑TCAGACGCTT‑TTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGGTGTTGTAAATTTTTTTCTGGGGTTTCATGTGCGCCAGCATGTTAGCTTTGCGCT‑GTTCTTCCATTTTTGCGCTCTGCGCTTCAGCTTTTACTTTTTTGAAGGTATc                     >  2:133191/1‑239 (MQ=255)                                                                                                                                                                                                               gACCGGCGG‑TCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTG‑CAGGCATTTTACCTTTTGCCGCTGGACGTTCT‑G‑TCAGACGCTT‑CTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAgg                                                                                                                                 <  2:1214965/132‑1 (MQ=255)                                                                                                                                                                                                               gACCGGCGG‑TCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTG‑CAGGCATTTTACCTTTTGCCGCTGGACGTTCT‑G‑TCAGACGCTT‑CTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAgg                                                                                                                                 >  1:1214965/1‑132 (MQ=255)                                                                                                                                                                                                               gACCGGCGG‑TCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTG‑CAGGCATTTTACCTTTTGCCGCTGGACGTTCT‑G‑TCAGACGCTT‑CTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGTTCTGGGTTTCCAtg                                                                                                   <  2:1742987/162‑1 (MQ=255)                                                                                                                                                                                                               gACCGGCGG‑TCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTG‑CAGGCATTTTACCTTTTGCCGCTGGACGTTCT‑G‑TCAGACGCTT‑CTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGTTCTGGGTTTCCAtg                                                                                                   >  1:1742987/1‑162 (MQ=255)                                                                                                                                                                                                                     ggg‑TCTTAATGTTGTTGGCTAAAAGATTATGCAGCAGTTG‑CAGGCATTTTACCTTTTGCCGCTGGACCTTCT‑G‑TCAGACGCTT‑CTCAAAATTAGCATTAAATTGCTTTTTCTGTGCCGGCGTCAGGATGTTGTAAATTTTGTTCGGGGTTTCCATGTGCGCCAGCATGTTAGCTTTTCGCT‑GTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGc                   <  2:780120/234‑1 (MQ=255)                                                                                                                                                                                                                     cgg‑CCTTAAGTTTTTTGGCTGAAAGAATAATCAGAAGGTG‑CAGGCATTTTACCTTCTGCCGCCGGCCGTTCT‑G‑TACGACGCCT‑CTCAAAATTACCATTACATTGCTTTTTCATTTCCCGCCGCAGTGTGATCTGAATTTTGTTCTTGGCATCTATCTGCTACATCATGTTAACTTTCCGCG‑GATTTTCCCTTTTTGCGATCTGCGCTTATGCGTTGACTTTCTCGCAGGTATTTCTGGc               >  2:1983851/1‑239 (MQ=255)                                                                                                                                                                                                                         tCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTG‑CAGGCATTTTACCTTTTGCCGCTGGACGtt                                                                                                                                                                                            <  1:1306869/67‑1 (MQ=255)                                                                                                                                                                                                                         tCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTG‑CAGGCATTTTACCTTTTGCCGCTGGACGTTCT‑T‑TCAGACGCTT‑CTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCT‑GTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCaa             <  1:1911871/238‑1 (MQ=255)                                                                                                                                                                                                                          tttAAGTTTTGTGGCTGAAAGATTTTTCAGCATTTG‑CAGGCATTTTACCTTTTGCCGCTGGACGTTCT‑G‑TCAGACGCTT‑CTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCT‑GTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAatg           <  2:1123271/238‑1 (MQ=255)                                                                                                                                                                                                                          cTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTG‑CAGGCATTTTACCTTTTGCCGCTGGACGTTCT‑G‑TCAGACGCTT‑CTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCT‑GTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAatg           <  1:133191/239‑1 (MQ=255)                                                                                                                                                                                                                          cTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTG‑CAGGCATTTTACCTTTTGCCGCTGGACGTTCT‑G‑TCAGACGCTT‑CTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCT‑GTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAatg           <  1:1470406/239‑1 (MQ=255)                                                                                                                                                                                                                           ttAAGTTTGTTGGCTGAAAGATTATTCCGCAGTTG‑CAGGCATTTTACCTTTTGCCGCTGGACGGTCT‑G‑TCAGACGCTT‑CTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCT‑GTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAatg           <  2:2024150/238‑1 (MQ=255)                                                                                                                                                                                                                            tAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTG‑CAGGCATTTTACCTTTTGCCGCTGGACGTTCT‑G‑TCAGACGCTT‑CTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGTTCTGGGTTTcc                                                                                                      >  1:263740/1‑147 (MQ=255)                                                                                                                                                                                                                            tAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTG‑CAGGCATTTTACCTTTTGCCGCTGGACGTTCT‑G‑TCAGACGCTT‑CTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGTTCTGGGTTTcc                                                                                                      <  2:263740/147‑1 (MQ=255)                                                                                                                                                                                                                                   tttGGTTGAAAGAGTATTCAGCAGAAG‑CAGGCCTTTTACCTTTTGCCGCTGGACGTTCT‑G‑TCAGACg                                                                                                                                                                                >  2:1306869/1‑67 (MQ=255)                                                                                                                                                                                                                                   tttGGCTGAATGATAATTCAGCAGTTG‑CGGGGAGTTTACCTTTTGCCGCTGGACGTTAT‑G‑TCAGAAGGTT‑CTAAAATTTAGCATTAAATTGCTTTTTTTTCTCCTGAGAAAGGATGTTTTAAATTTTGTTCTCGGTTTCCATGTGCGCCAGCATGTTAGATTTGCGCT‑GACATTCCTTTTTTTCGACCTCCGGACCCGCTCTCAATTTATCGAGGGTAACACTGGGAatcgaggcactc  >  2:708429/1‑229 (MQ=255)                                                                                                                                                                                                                                   tttGGCTGAAAGATTATTCAGCAGTTG‑CAGGCATTTTACCTTTTGCCGCTGGACGTTCT‑G‑TCAGACGCTT‑CTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGTTCt                                                                                                              >  2:803679/1‑132 (MQ=255)                                                                                                                                                                                                                                   tttGGCTGAAAGATTATTCAGCAGTTG‑CAGGCATTTTACCTTTTGCCGCTGGACGTTCT‑G‑TCAGACGCTT‑CTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGTTCt                                                                                                              <  1:803679/132‑1 (MQ=255)                                                                                                                                                                                                                                   tttGGCTGAAAGATTATTCAGCAGTTG‑CAGGCATTTTACCTTTTGCCGCTGGACGTTCT‑G‑TCAGACGCTT‑CTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGt                                                                                      >  2:616839/1‑156 (MQ=255)                                                                                                                                                                                                                                   tttGGCTGAAAGATTATTCAGCAGTTG‑CAGGCATTTTACCTTTTGCCGCTGGACGTTCT‑G‑TCAGACGCTT‑CTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGt                                                                                      <  1:616839/156‑1 (MQ=255)                                                                                                                                                                                                                                   tttGGCTGAAAGATTATTCAGCAGTTG‑CAGGCATTTTACCTTTTGCCGCTGGACGTTCT‑G‑TCAGACGCTT‑CTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCT‑GTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGc  >  1:1123271/1‑239 (MQ=255)                                                                                                                                                                                                                                   tttGGCTGAAAGATTATTCAGCAGGTG‑CAGGCATTTTACCTTTTGCCGCTGGACGTTCT‑G‑TCAGACGCTT‑CTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCT‑GTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGCAGGTATCGCTGGCAATTATGTCATGc  >  2:1278892/1‑239 (MQ=255)                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                 ATTCACCGACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGG‑TCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTG‑CAGGCATTTTACCTTTTGCCGCTGGACGTTCT‑G‑TCAGACGCTT‑CTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCT‑GTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGC  >  NC_000913/1824902‑1825365

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑