Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,745,397	0	A	C	36.4%	95.8 / 11.2	57	L198F (TTA→TTC)	yiaN	2,3‑diketo‑L‑gulonate TRAP transporter large permease protein
Reads supporting (aligned to +/- strand):  ref base A (29/6);  new base C (0/20);  total (30/27)
Fisher's exact test for biased strand distribution p-value = 4.56e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CCGATGATGCGCAGCGCCAACTATCCGGTCAACCGGGCGGCGGGGCTGATTGCCTCTGGCGGCATTATCGCGCCAATTATTCCCCCTTCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAA‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTATGCGCTGTTTGTCGCCACAGTTATTTACCGTGAAATGACCTTCGCCACACTTTGGCATGTACTCATTGGCGCGGCGAA  >  NC_000913/3745173‑3745621                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                 ccGATGATGCGCAGCGCCAACTATCCGGTCAACCGGGCGGCGGGGCTGATTGCCTCTGGCGGCATTATCGCGCCAATTATTCCCCCTTCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCCTTGGCCCCGGCATGATGATGGGCGCAACGCCGATGCTTACCTGGTGGTGGGAGGACACTCGGCTTTA‑TTTACCCCCCCAGCaaaa                                                                                                                                                                                                                    >  1:1432480/1‑239 (MQ=255) ccGATGATGCGCAGCGCCAACTATCCGGTCAACCGGGCGGCGGGGCTGATTGCCTCTGGCGGCATTATCGCGCCAATTATTCCCCCTTCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTTCCCCTGGCATGATGATGGGGGCCACACTGATGCTTACCTGGTGGGGGCAGGCCAGGCGCCTTAA‑TTTTCCCCGCCAGCaaaa                                                                                                                                                                                                                    >  1:2021403/1‑239 (MQ=255)                                                 tgtGCTTCGGGCGCCGTTAGCGTGCAATTTATTCCCCCGTCCTTTCCTTTTATTTTCTTCGGCTTTTCCACAGGATTTGCCAGCTATAAGCTTTTTAGGGGGGCCTTCCCCCCGGGCTTGTTAGTGGGGGCACCGGTGATCCTTCCCGGGTGGTGGCAGGCCAGTCGCTTTAA‑TTTTCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGgc                                                                                                                                                                    <  2:1717488/237‑1 (MQ=255)                                                                              tattCCCCCTCCCATCCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAA‑TTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCa                                                                                                                                                       <  1:1902173/223‑1 (MQ=255)                                                                                           aTTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCCTTGCCCCCGGGATGATTTTGGGCGGCAAGCTGATGCTTAGCTGGGGGGGGCCGGCCCGTCCCCTTAA‑TTTACCCCGCGAGCCAAAAACAAAGATGCACGCAAACTGGGAATCCTTTTTTCCCGGTATCTTGGCGCTTGTTCTTCCCGGTATCATTCTTGTCggggggtggtcgc                                                                                                                           >  2:780147/1‑228 (MQ=255)                                                                                                 tttATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTGCCCTGGGCATGATGATGGGCGCAACGCTGATGCTTCCCTGGTGGTGGCAGGCCAGTCGCCTTAA‑TTTACCCCGCCAGCAAAAAGCAACGa                                                                                                                                                                                                            <  1:483059/151‑1 (MQ=255)                                                                                                  ttattaTCTTCGGCGTTTCCGGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTGCCCCTGGCATGATGATGGGCGCAGCGCTGATGCTTACCTGGTGGGGGCAGGCCAGTCGCCTTAA‑TTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGtt                                                                                                                    <  1:1812677/238‑1 (MQ=255)                                                                                                                               tCCATCAGTAAGCTGTTTATGGCGGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAA‑TTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGgc                                                                                                                                                                    <  1:58001/161‑1 (MQ=255)                                                                                                                               tCCATCAGTAAGCTGTTTATGGCGGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGGGGCAGGCCAGTCGCTTTAA‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGtctc                                                                                                                                                                                <  1:86028/149‑1 (MQ=255)                                                                                                                                    cAGTAAGCTGTTTATGGCGGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAA‑TTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCt                                                                                                                                                                 <  1:1437924/159‑1 (MQ=255)                                                                                                                                         aGCTGTTTATGGCGGGCATTGTCCCTGGCATGATGATGGGCGCAAAGCTGATGCTTACCTGGGGGTGGGAGGGCAGGCGCCTTAA‑TTTACCCCGCCCGCAAAAAGCAACGAAGGAGGAAATCTGGCACTCTTTTGTCTCCGGGATCTGGGCGGTGGttctt                                                                                                                                                          >  2:58001/1‑161 (MQ=255)                                                                                                                                         aGCTGTTTATGGCGGGCATTGCCCCTGGCATGATGATGGGCGCCACGCTGATGCTTTCCTGGGGGGGGCAGGGCAGTCGCCCTTA‑TTTACCCCCCCCGCAAAAAACAACGATGGAGGAAATCTGGCACTCCTTTGTCTCCGGGATCTgg                                                                                                                                                                      >  2:86028/1‑149 (MQ=255)                                                                                                                                                       ggtttgtcccgTGGCAGGGTGGTGGGCGCAACGTTTGTGTATACCGGGTGGCGGCGGGCCACTCGCCTTAA‑TTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTATGCGCTGTTTGTCGCca                                                               <  2:2021403/231‑1 (MQ=255)                                                                                                                                                            tGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAA‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGgc                                                                                                                                                                    >  1:1572936/1‑132 (MQ=255)                                                                                                                                                                   ggCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAA‑TTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGtt                                                                                                                                   <  1:802263/158‑1 (MQ=255)                                                                                                                                                                    gCATGAGGATGGGCGCAACGCTGATGCTTACCGGGGGGTGGCAGGCCATTCGCCTTAA‑TTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCt                                                                                                                                                                 <  2:1206135/127‑1 (MQ=255)                                                                                                                                                                        gatgatgGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAA‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGgc                                                                                                                                                                    >  1:1698495/1‑120 (MQ=255)                                                                                                                                                                        gatgatgGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAA‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGgc                                                                                                                                                                    >  1:1100024/1‑120 (MQ=255)                                                                                                                                                                         atgatgGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAA‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGgcg                                                                                                                                                                   >  1:1236647/1‑120 (MQ=255)                                                                                                                                                                         atgatgGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAA‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGgcg                                                                                                                                                                   <  2:1236647/120‑1 (MQ=255)                                                                                                                                                                          tgatgGGCGCAACGCTGATGCTTACCGGGTGGGGGCAGGCCAGTCGCCTTAA‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTATGCGCTGTTTGTCGc                                                                 <  1:2134649/217‑1 (MQ=255)                                                                                                                                                                             tggcctcacctcTGATGCTTCCCGGGTGGTGGCCGGCCAGTCGCCTTAA‑TTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTTTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTATGCGCTGTTTGTCGCCACAGTTATTTACCGTGAAATGAc                                         <  1:698928/227‑1 (MQ=255)                                                                                                                                                                             tgGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAA‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGgc                                                                                                                                                                    >  2:802263/1‑115 (MQ=255)                                                                                                                                                                              gggCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAA‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGgc                                                                                                                                                                    >  1:1501580/1‑114 (MQ=255)                                                                                                                                                                              gggCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAA‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGgc                                                                                                                                                                    >  1:268698/1‑114 (MQ=255)                                                                                                                                                                              gggCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAA‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGgc                                                                                                                                                                    >  1:103727/1‑114 (MQ=255)                                                                                                                                                                              gggCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAA‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCa                                                                                                                                                       >  1:1206135/1‑127 (MQ=255)                                                                                                                                                                                gcgcACCGCTGATGCTTCCCTGGTGGGGGCAGGCCAGTCGCCTTAA‑TTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGcc                                                                                                    <  1:1967590/176‑1 (MQ=255)                                                                                                                                                                                   cAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAA‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGgc                                                                                                                                                                    >  1:305416/1‑109 (MQ=255)                                                                                                                                                                                    aaCGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAA‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTggcc                                                                                                                                                                    >  2:2134649/1‑106 (MQ=255)                                                                                                                                                                                          gATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAA‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGgc                                                                                                                                                                    >  2:1967590/1‑102 (MQ=255)                                                                                                                                                                                             tcTTCCCGGGTGGTGCCGCGCCAGTCCCCTTAA‑TTCACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTATGCGCTGTTTGTCGCCACAGTTATTTACCGTGAAATGACCTTCGCCACACTTTGGc                        <  2:1572936/238‑1 (MQ=255)                                                                                                                                                                                             tcTTACCTGTTGGGGGACGCCCAGTCCCCTTAA‑TTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCgg                                                                                                                          <  2:1512813/140‑1 (MQ=255)                                                                                                                                                                                             gCTTCCCTGGTGGGGGCGGGCGAGTCGCCTAAA‑TTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCa                                                                                                                                                       <  2:916429/112‑1 (MQ=255)                                                                                                                                                                                             gCTTCCCTGGGGGGGGCAGGCCAGTCGCCTTAA‑TTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGt                                                                                                                                                     <  1:1580168/114‑1 (MQ=255)                                                                                                                                                                                             gCTTCCCGGGTGGGGGCAGGCCAGTCCCTTTAA‑TTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTATGCGCTGTTTGTCGCCACAGTTATTTACCGTGAAATGACCTTCGCCACACTTTGGc                        <  1:36017/239‑1 (MQ=255)                                                                                                                                                                                             gCTTACCTGGGGGGGGCAGGCCAGCCGCCTTAA‑TTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTATGCGCTGTTTGTCGCCACAGTTATTTACCGTGAAATGACCTTCGCCACACTTTGGc                        <  1:1554216/239‑1 (MQ=255)                                                                                                                                                                                              cTTGCCGGGTCGGGGCAGGCCGTTCTCCTTAA‑TTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCGCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTCCCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTATGCGCTGTTTGTCGCCACAGTTATTTACCGTGAAATGACCTTCGCCACACTTTGGc                        <  2:305416/238‑1 (MQ=255)                                                                                                                                                                                              cTTCCCTGGTGGTGGCAGGCAGGTCGCCTTAA‑TTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTg                                                                                                                                          <  1:373641/124‑1 (MQ=255)                                                                                                                                                                                              cTTACCTGGTGGTGGCAGGCCAGTCGCCTTAA‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGgc                                                                                                                                                                    >  2:414927/1‑98 (MQ=255)                                                                                                                                                                                                tcccgggtggtggCAGG‑CAGGCCCCTTAATTTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCCCTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTATGCGCTGTTTGTCGCCACAGTTATTTACCGTGAAATGACCTTCGCCACACTTTGGCATg                     <  1:32347/234‑1 (MQ=255)                                                                                                                                                                                                 aCCTGGTGGTGGCAGGCCAGTCGCCTTAA‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGgc                                                                                                                                                                    >  2:1101386/1‑95 (MQ=255)                                                                                                                                                                                                      gtggtggCAGGCCAGTCGCCTTAA‑TTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGc                                                                                                                         <  1:761847/133‑1 (MQ=255)                                                                                                                                                                                                       tggtggCAGGCCAGTCGCCTTAA‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGgc                                                                                                                                                                    >  1:916429/1‑89 (MQ=255)                                                                                                                                                                                                       tggtggCAGGCCAGTCGCCTTAA‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGgc                                                                                                                                                                    >  1:1512813/1‑89 (MQ=255)                                                                                                                                                                                                       tggtggCAGGCCAGTCGCCTTAA‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGgc                                                                                                                                                                    >  2:1580168/1‑89 (MQ=255)                                                                                                                                                                                                       tggtggCAGGCCAGTCGCCTTAA‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGgc                                                                                                                                                                    >  1:1983436/1‑89 (MQ=255)                                                                                                                                                                                                       tggtggCAGGCCAGTCGCCTTAA‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGgc                                                                                                                                                                    >  1:2066774/1‑89 (MQ=255)                                                                                                                                                                                                        ggtggCAGGCCAGTCGCCTTAA‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGgc                                                                                                                                                                    >  2:373641/1‑88 (MQ=255)                                                                                                                                                                                                           ggCAGGCCAGTCGCCTTAA‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGgc                                                                                                                                                                    >  1:968568/1‑85 (MQ=255)                                                                                                                                                                                                           ggCAGGCCAGTCGCCTTAA‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGgc                                                                                                                                                                    >  1:280222/1‑85 (MQ=255)                                                                                                                                                                                                           ggCAGGCCAGTCGCCTTAA‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGAAAATCTGGCACTCTTTTGACTCCGGTATATGGgc                                                                                                                                                                    >  2:167291/1‑85 (MQ=255)                                                                                                                                                                                                                gCCAGTCGCCTTAA‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGgcg                                                                                                                                                                   >  2:1088000/1‑81 (MQ=255)                                                                                                                                                                                                                gCCAGTCGCCTTAA‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGgcg                                                                                                                                                                   <  1:1088000/81‑1 (MQ=255)                                                                                                                                                                                                                gCCAGTCGCCTTAA‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGgc                                                                                                                                                                    >  2:761847/1‑80 (MQ=255)                                                                                                                                                                                                                   tgcccctttAA‑TTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTATGCGCTGTTTGTCGCCACAGTTATTTACCGTGAAATGACCTTCGCCACACTTTGGCATGTACTCATTGGCGCGGCGaa  <  1:674726/232‑1 (MQ=255)                                                                                                                                                                                                                      cGCCTTAA‑TTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTCCCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTATGCGCTGTTTGTCGCCACAGTTATTTACCGTGAAATGACCTTCGCCACACTTTGGCATGTACTCATTGGCgcggcg    <  1:414927/234‑1 (MQ=255)                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                 CCGATGATGCGCAGCGCCAACTATCCGGTCAACCGGGCGGCGGGGCTGATTGCCTCTGGCGGCATTATCGCGCCAATTATTCCCCCTTCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAA‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTATGCGCTGTTTGTCGCCACAGTTATTTACCGTGAAATGACCTTCGCCACACTTTGGCATGTACTCATTGGCGCGGCGAA  >  NC_000913/3745173‑3745621

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑