| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,793,897 | Δ1 bp | 100% | coding (213/552 nt) | btuE ← | glutathione peroxidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,793,897 | 0 | T | . | 98.3% | 263.0 / ‑3.2 | 58 | coding (213/552 nt) | btuE | glutathione peroxidase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base . (31/26); minor base A (0/1); total (31/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.66e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTGAGGCGACATTGACAATCAACAGCACATTACCGGCG > NC_000913/1793749‑1794041 | gcgcGACTGCGGTCGGCGCTGCGGATATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCt > 1:680367/1‑149 (MQ=255) aCTGCGGTCGGCGCTGCGGCAATCAATTTATGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTAGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTcc > 1:952086/1‑149 (MQ=255) gCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGa < 1:128491/149‑1 (MQ=255) gTCGGCGCTGCGTCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAAc < 2:187011/149‑1 (MQ=255) tCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACCTCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGCAACt > 2:1208337/1‑149 (MQ=255) ggCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGCAACTgg > 2:441533/1‑149 (MQ=255) gCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCAc > 1:1103146/1‑149 (MQ=255) cGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTACTCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACg < 2:1307150/149‑1 (MQ=255) cGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACg < 1:899803/149‑1 (MQ=255) atcaatTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAAt > 1:151973/1‑149 (MQ=255) atcaatTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAAt > 2:88321/1‑149 (MQ=255) atcaatTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAAt > 2:913215/1‑149 (MQ=255) tcaatTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATc > 2:1003078/1‑149 (MQ=255) aatTTTTGATACCGCGGACGGCGTCCTTCGTCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATccc < 2:764977/149‑1 (MQ=255) aatTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATccc < 2:547671/149‑1 (MQ=255) aatGTTTGATACAGCGGCTGGCGGCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGGTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATccc < 1:80600/149‑1 (MQ=255) atTTTTGATACAGCGGATGGCGTCCTTCGTCATTAACTTCAATCTTACTGAACATCGGGAACGTCACACCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCGAGAAACTGGTTGCACGGGAATCCCa > 1:821314/1‑149 (MQ=255) atTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGCACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCa < 2:763779/149‑1 (MQ=255) atTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCa < 1:572084/149‑1 (MQ=255) ttttcGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTGACTGAACATCTGGACCGTCACCCCCCATGTGGTGGGACAGTAAGTTTTAATCGCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAg < 2:568838/149‑1 (MQ=255) gATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCa > 1:81980/1‑149 (MQ=255) aTACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCAt > 1:624674/1‑149 (MQ=255) cGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAAcc > 2:990372/1‑149 (MQ=255) ggATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCt < 1:672218/149‑1 (MQ=255) gATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGCTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCtc < 1:914328/149‑1 (MQ=255) tCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCCCGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATACAACCTCGATCAACCCAg > 2:301905/1‑149 (MQ=255) cGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAgg > 1:916536/1‑149 (MQ=255) ttggcTTCTATCTTGCCGACCATTTAGAACCCCACCCCGCATGTGGTGGTACAGTAAGTTTTAATCTCGTCATCGCTGCCCGGTTC‑TGTGCCAGAAACTGGTTGCACGGGAATCCCGGCACCATAAAACCTCGATCGTCCCAGGCTTTc < 2:1287789/145‑1 (MQ=255) cTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGaa < 2:1161578/149‑1 (MQ=255) tCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAata < 1:612588/149‑1 (MQ=255) tCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAata > 2:1047549/1‑149 (MQ=255) tCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGCTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCCCCATAAAACCTCGATGAACCCAGGCATTCTGAatc > 2:1047531/1‑148 (MQ=255) cAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCCGGGGAATCCGAGCACCATAAAACCTCGATAGGCCCAGGCTTTCTGAatat > 1:408288/1‑149 (MQ=255) tCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATAGtct > 2:1237862/1‑149 (MQ=255) aCTGACCATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCaa < 1:547304/149‑1 (MQ=255) aCTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCaa < 1:546585/149‑1 (MQ=255) aCTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCaa < 2:420177/149‑1 (MQ=255) tGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACt > 1:1118853/1‑149 (MQ=255) tGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGCAAGGTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAGCt > 1:51768/1‑149 (MQ=255) gAACATCGGGAACGTCACCCCCCAGGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTg < 2:1254263/149‑1 (MQ=255) aTCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCa < 1:763800/149‑1 (MQ=255) tCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCCCat > 1:54246/1‑149 (MQ=255) gggAACGTCACCCCCCATGTGGTGGTACAGAAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATACCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGACTATTTTCCAACTGCTCatat > 1:703277/1‑149 (MQ=255) ggAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATAtt > 1:857740/1‑149 (MQ=255) aaCGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAACCTGGTTGCACGGGAATCTCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACCGCTCATATTgc > 2:772011/1‑149 (MQ=255) aCGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTgcg < 1:306637/149‑1 (MQ=255) ccATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGcc > 1:621005/1‑149 (MQ=255) tggtggTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAATCTGGTTGCAAGAGCTTCCCAGCACCATCATGCCTCTACCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACt < 1:990372/149‑1 (MQ=255) tACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTGAgg < 2:916536/149‑1 (MQ=255) tAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTGAGGCGACa > 1:11389/1‑149 (MQ=255) aGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCCTAAAACCTCGATCGACCCAGGCTTTCTGACTCTTCTCCAACTGCTCATATTGCGGCGTTAAGCCCCACTTTGAGTCGACAtt > 1:1085700/1‑149 (MQ=255) aTCTCTTCCCCGCTGCCC‑CTTTATGCTCTATAAACTGGTTGCACGTGAATCCCATCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTGAGGCGACATTGACGATc < 2:1225980/149‑1 (MQ=255) ctctTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTGAGGCGACATTGACAATCaa < 1:30062/149‑1 (MQ=255) tctTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGCATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTGAGGCGCCATTGACACTCCAc > 2:579162/1‑149 (MQ=255) cATCGCCGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGTGAATCCTAGCACGCTATATCCTGGAGCGACGCAGGCTTTCTGAGGTGACGCCATCTACTCTTATTGCGGCGTTAAGCCACTCTTGGAGGCGACATGGACTAGCATCAGca < 2:51768/149‑1 (MQ=255) gCTGCCCGGTTC‑TGTTCCAGAAACTAGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTGAGGCGACATTGACAATCAACAGCACAtt > 1:679448/1‑149 (MQ=255) tGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGGTCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTACGGCGTTAAGCCACACTTTGAGGCGACATTACCAATCCACAGCACCTTAc > 1:273509/1‑149 (MQ=255) cccGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTGAGGCGACCTTGACAATCAACAGCACATTACCg > 2:21653/1‑149 (MQ=255) ggTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTGAGGCGACATTGACAATCAACAGCACATTACCGGCg < 1:913215/149‑1 (MQ=255) | GCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTGAGGCGACATTGACAATCAACAGCACATTACCGGCG > NC_000913/1793749‑1794041 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |