| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 180,794 | T→C | 6.8% | intergenic (+40/‑90) | dgt → / → degP | deoxyguanosine triphosphate triphosphohydrolase/serine endoprotease (protease Do), membrane‑associated |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 180,794 | 0 | T | C | 6.8% | 161.1 / 3.1 | 61 | intergenic (+40/‑90) | dgt/degP | deoxyguanosine triphosphate triphosphohydrolase/serine endoprotease (protease Do), membrane‑associated |
| Reads supporting (aligned to +/- strand): ref base T (30/26); new base C (2/2); total (33/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.17e-01 | |||||||||||
CTATGGGAATATTATTACCGTTGCCGCCTGCTGCAGGATTATATCAGCGGTATGACCGACCTCTATGCGTGGGATGAATACCGACGTCTGATGGCCGTAGAACAATAACCAGGCTTTTGTAAAGACGAACAATAAATTTTTACCTTTTGCAGAAACTTTAGTTCGGAACTTCAGGCT‑ATAAAACGAATCTGAAGAACACAGCAATTTTGCGTTATCTGTTAATCGAGACTGAAATACATG‑AAAAAAACCACATTAGCACTGAGTGCACTGGCTCTGAGTTTAGGTTTGGC > NC_000913/180647‑180936 | cTATGGGAATATTATTACCGTTGCCGCCTGCTGCAGGATTATATCAGCGGTATGACCGACCTCTATGCGTGGGATGAATACCGACGTCTGATGGCCGTAGAACAATAACCAGGCTTTTGTAAAGACGAACAATAAATTTTTACCTTTTg < 1:7575/149‑1 (MQ=255) atatTATTACCGTTGCCGCCTGCTGCAGGATTATATCAGCGGTATGACCGACCTCTATGCGTGGGATGAATACCGACGTCTGATGGCCGTAGAACAATAACCAGGCTTTTGTAAAGACGAACAATAAATTTTTACCTTTTGCAGAAACt > 1:722680/1‑149 (MQ=255) tattatTCCCGTTGCCGCCTGCTGCAGGATTATATCAGCGGTATGACCGACCTCTATGCGTGGGATGAATACCGACGTCTGATGGCCGTAGAACAATAACCAGGCTTTTGTAAAGACGAACAATAAATTTTTACCTTTTGCAGAAACtt < 1:103769/149‑1 (MQ=255) ttaCCGTTGCCGCCTGCGGCAGGATTATACCAGCTGTATGACCCACCTCTACTCGTGGGATGAATACCGACGTCGGATGGCCGTAGAACAATAACCAGGCTTTTGTAAATACGAACAATAAATTTTTACCTTTTGCAGAAACCTTAGtt < 2:292903/149‑1 (MQ=255) gTTGCCGCCTGCTGCAGGATTATATCAGTGTTATGACGGACCTCTATGCGTGGGATGAATACCGACGTCTGATGGCCGTAGAACAATAACCAGGCTTTTGTAAAGACGAACAACACATTTTTACCTTTTGCAGAAACTTTAGTTCAGac < 2:562241/149‑2 (MQ=255) ccgccTGCTGCAGGATTATATCAGCGGTATGACCGACCTCTATGCGTGGGATGAATACCGACGTCTGATGGCCGTAGAACAATAACCAGGCTTTTGTAAAGACGAACAATAAATTTTTACCTGTTGCAGAAACTTTAGGGCGGAACTTc > 2:631218/1‑149 (MQ=255) cgccTGCTGCAGGATTATATCAGCGGTATGACCGACCTCTATGCGTGGGATGAATACCGACGTCTGATGGCCGTAGAACAATAACCAGGCCTTTGTCAAGACGAACAATAAATTTTTACCTTTTGCAGAAACTTTAGTTCGGAACTTcc > 1:685673/1‑148 (MQ=255) ccTGCTGCAGGATTATATCAGCGGTATGACCGACCTCTATGCGTGGGATGAATACCGACGTCTGATGGCCGTAGAACAATAACCAGGCTTTTGTAAAGACGCACAATAAATTTTTACCTTCTGGAGAAACTTTAGTTCGGAACTTCAgg < 1:448441/149‑1 (MQ=255) ccTGCTGCAGGATTATATCAGCGGTATGACCGACCTCTATGCGTGGGATGAATACCGACGTCTGATGGCCGTAGAACAATAACCAGGCTTTTGTAAAGACGAACAATAAATTTTTACCTTTTGCAGAAACTTTAGTTCGGAACTTCAgg > 2:161715/1‑149 (MQ=255) ccTGCTGCAGGATTATATCAGCGGTATGACCGACCTCTATGCGTGGGATGAATACCGACGTCTGATGGCCGTAGAACAATAACCAGGCTTTTGTAAAGACGAACAATAAATTTTTACCTGTTGCAGAAACTTTAGTTCGGAACTTCAgg > 2:161567/1‑149 (MQ=255) cAGGATTATATCAGCGGTATGACCGACCTCTATGCGTGGGATGAATACCGACGTCTGATGGCCGTAGAACAATAACCAGGCTTTTGTAAAGACGAACAATAAATTTTTACCTTTCGCAGAAACTTTAGTTCGGAACTTCAGGCT‑ATaaa > 2:566185/1‑149 (MQ=255) cAGGATTATATCAGCGGTATGACCGACCTCTATGCGTGGGATGAATACCGACGTCTGATGGCCGTAGAACAATAACCAGGCTTTTGTAAAGACGAACAATAAATTTTTACCTTTCGCAGAAACTTTAGTTCGGAACTTCAGGCT‑ATaaa > 2:575885/1‑149 (MQ=255) gaatataTCAGCGGTATGACCGACCTCTATGCGTGGGATGAATACCGACGTCTGATGGCCGTAGAACAATAACCAGGCTTTTGTAAAGACGAACAATAAATTTTTACCGTTTGCAGAAACTTTAGTTCGCAACTTCAGGCT‑ATAAAACg > 1:722331/4‑149 (MQ=255) gATTATATCAGCGGTATGACCGAGCTATATGCGTGGGATGAAGACCGACGTCTGATGGCCGTAGAACGATAGCCAGGCTGTTGTAAAAACGAACAATACGTCTTTGCCGTTTGCAGAAAGTTTCGGTCGGACCTTCAGGTC‑ATATAACg > 2:446621/1‑149 (MQ=255) gATTATATCAGCGGTATGACCGACCTCTATGCGTGGGATGAATACCGACGTCTGATGGCCGTAGAACAATAACCAGGCTTTTGTAAAGACGAACAATAAATTTTTACCTTTTGCAGAAACTTTAGTTCGGAACTTCAGGCT‑ATAAAACg > 1:89013/1‑149 (MQ=255) tataTCAGCGGTATGACCGACCTCTATGCGTGGGATGAATACCGACGTCTGATGGCCGTAGAACAATAACCAGGCTTTTGTAAAGACGAACAATAAATTTTTACCTTTTGCAGAAACTTTAGTTCGGAACTTCAGGCT‑ATAAAACGAAt < 2:658261/149‑1 (MQ=255) atatCAGCGGTATGACCGACCTCTATGCGTGGGATGAATACCGACGTCTGATGGCAGTAGAACAATAACCAGGCTTTTGTAAAGACGAACAATAAATTTTTACCTTTTGCAGAAACTTTAGTTCGGAACTTCAGGCT‑CTAAAACGAATc > 1:615453/1‑149 (MQ=255) gCGGTATGACCGACCTCTATGCGTGGGATGAATACCGACGTCTGATGGCCGTAGAACAATAACCAGGCTTTTGTAAAGACGAACAATAAATTTTTACCTTTTGCAGAAACTTTAGTTCGGAACTTCAGGCT‑ATAAAACGAATCTgaaga > 1:185479/1‑149 (MQ=255) gCGGTATGACCGACCTCTATGCGTGGGAAGAAGACCGATGTCTGATGGCCGTAGAACAATAACCAGGCTTTTGTAAAGACGAACAATAAATTTTTACCTTTTGCAGAAACTTTAGTTCGGAACTTCAGGCT‑ATAAAACGAATCTgcaga > 2:174425/1‑149 (MQ=255) cGGTATGACCGACCTCTATGCGTGGGATGAATACCGACGTCTGGTGGCCGTAGAACAATAACCAGGCTTTTGTACAGCCGAACAATGAATTTTTACCTTTTGCAGAAACTTTAGTGCGGAACTTCAGGCT‑ATAAAACGAATCTgaagaa > 2:684288/1‑149 (MQ=255) cGGTATGACCGACCTCTATGCGTGGGATGAATACCGACGTCTGATGGCCGTAGAACAATAACCAGGCTTTTGTAAAGACGAACAATAAATTTTTACCTTTTGCAGAAACTTTAGTTCGGAACTTCAGGCT‑ATAAAACGAATCTgaagaa > 2:162038/1‑149 (MQ=255) accgaccTCTATGCGTGGGATGAATACCGACGTCTGATGGCCGTAGAACAATAACCAGGCTTTTGTAAAGACGAACAATAAATTTTTACCTTTTGCAGAAGCTTTAGTTCGGAACTTAAGACT‑ATAAAACGAATCTGAAGAACACAGCa > 2:348495/1‑149 (MQ=255) cgaccTCTATGTGTGGGATGAATACCGACGTCTGATGGCCGTAGAACAATAACCAGGCTTTTGTAAAGACGAACAATAAATTTTTACCTTTTGCAGAAACTTTAGTTCGGAACTTCAGGCT‑ATAAAACGAATCTGGAGAACACAGCagt > 1:21570/1‑147 (MQ=255) gaccTCTATGCGTGGGATGAATACCGACGTCTGATGGCCGTAGAACAATAACCATGCTTTTGTAAAGACGAACAATAGATTTTTACCTTTTGCAGAAACTTTAGTTCGGAACTTCAGGCT‑ATAAAACGAATCTGAAGAACACAGCAAtt < 2:374989/149‑1 (MQ=255) gaccTCTATGCGTGGGATGAATACCGACGTCTGATGGCCGTAGAACAATAACCAGGCTTTTGTAAAGACGAACAATAAATTTTTACCTTTTGCAGAAACTTTAGTTCGGAACTTCAGGCT‑ATAAAACGAATCTGAAGAACACAGCAAtt > 2:388495/1‑149 (MQ=255) ctctATGCGTGGGATGAATACCGACGTCTGATGGCCGTAGAACAATAACCAGGCTTTTGTAAAGACGAACAATAAATTTTTCCCTTTTGCAGAAACTTTAGTTCGGAACTTCAGGCT‑ATATAACGAATCTGAAGAACACAGCAATTTTg < 1:480664/149‑1 (MQ=255) cactATGCATGGGATGAATACCGACGTCGGATGGCCGTAGGACAAGAACCATGCGTTGGGAAAGACGAACAATAAATTTTTACCTTTTGCAGAAACTTTAGTTCGGAACTTCAGGCT‑ATAAAACGAATCTGAAGAACACAGCAACTTTg > 2:469016/3‑149 (MQ=255) ctATGCGTGGGATGAATACCGACTTCTGATGGCCGTAGAACAATAACCAGGCTTTTGTAAAGACGAACAATAAATTTTTACCTTTTGCAGAAACTTTAGTTCGGAACTTCAGGCT‑ATAAAACGAATCTGAAGAACACAGCAATTTTGCg > 1:443833/1‑149 (MQ=255) cGTGGGATGAATACCGACGTCTGATGGCCGTAGAACAATAACCAGGCTTTTGTAAAGACGAACAATAAATTTTTACCTTTTGCAGAAACTTTAGTTCGGAACTTCAGGCT‑ATAAAACGAATCTGAAGAACACAGCACTTTTGCGTTATc > 1:32276/1‑149 (MQ=255) cGTGGGATGAATACCGACGTCTGATGGCCGTAGAACAATAACCAGGCTTTTGTAAAGACGAACAATAAATTTTTACCTTTTGCAGAAACTTTAGTTCGGAACTTCAGGCT‑ATAAAACGAATCTGAAGAACACAGCAATTTTGCGTTATc < 2:375639/149‑1 (MQ=255) cGTGGGATGAATACCGACGTCTGATGGCCGTAGAACAATAACCAGGCTTTTGTAAAGACGAACAATAAATTTTTACCTTTTGCAGAAACTTTAGTTCGGAACTTCAGGCT‑ATAAAACGAATCTGAAGAACACAGCAAGTTTGCGTTATc > 1:32190/1‑149 (MQ=255) gcaggATGAATACCGACGTCTGATGGCCGTAGAACAATAACCAGGCTTTTGTAAAGACGAACAATAAATTTTTACCTTTTGCAGAAACTTTAGTTCGGAACTTCAGGCT‑ATAAAACGAATCTGAAGAACACAGCAATTTTGCGTTATcc > 2:336497/4‑148 (MQ=255) taTACCGACGTCTGATGGCCGTAGAACAATAACCAGGCTTTTGTAAAGACGAACAATAAATTTTTACCTTTTGCAGAAACTTTAGTTCGGAACTTCAGGCT‑ATAAAACGAATCTGAAGAACACAGCAATTTTGCGTTATCTGTTAATCg < 2:385820/148‑1 (MQ=255) tcaCCGATGTCTGATGGCCGTAGAACAATAACCAGGCTTTTGTAAAGACGAACAATAAATTTTTACCTTTTGCAGAAACGTTAGTTCGGAACTTCAGGCT‑ATAAAACGAATCTGAAGAACACAGCAATTTTGCGTTATCTGTTAATCga < 2:631758/147‑1 (MQ=255) aTACCGACGTCTGATGGCCGTAGAACAATAACCAGGCTTTTGTAAAGACGAACAATAAATTTTTACCTTTTGCAGAAACTTTAGTTCGGAACTTCAGGCT‑ATAAAACGAATCTGAAGAACACAGCAATTTTGCGTTATCTGTTAATCga < 1:283940/149‑1 (MQ=255) aCCGACGTCTGATGGCCGTAGAACAATAACCAGGCTTTTGTAAAGACGAACAATAAATTTTTACCTTTTGCAGAAACTTTAGTTCGGAACTTCAGGCT‑ATAAAACGAATCTGAAGAACACAGCAATTTTGCGTTATCTGTTAATCgaga > 1:576937/1‑149 (MQ=255) aTGGCCGTAGAACAATAACCAGGCTTTTGTAAAGACGAACAATAAATTTTTACCTTTTGCAGAAACTTTAGTTCGGAACTTCAGGCT‑ATAAAACGAATCTGAAGAACACAGCAATTTTGCGTTATCTGTTAATCGAGACTGAAATACAt < 1:722062/149‑1 (MQ=255) aTGGCCGTAGAACAATAACCAGGCTTTTGTAAAGACGAACAATAAATTTTTACCTTTTGCAGAAACTTTAGTTCGGAACTTCAGGCT‑ATAAAACGAATCTGAAGAACACAGCAATTTTGCGTTATCTGTTAATCGAGACTGAAATACAt < 1:333526/149‑1 (MQ=255) gCCGTAGAACAATAACCAGGCTTTTGTAAAGACGAACAATAAATTTTTACCTTTTGCAGAAACTTTAGTTCGGAACTTCAGGCT‑ATAAAACGAATCTGAAGAACACAGCAATTTTGCGTTATCTGTTAATCGAGACTGAAATACATG‑aa > 1:437951/1‑149 (MQ=255) cGTAGAACAATAACCAGGCTTTTGTAAAGACGAACAATAAATTTTTACCTTTTGCAGAAACTTTAGGTCGGAACTTCAGGCT‑ATAAAACGAATCTGAAGAACACAGCAATTTTGCGATATCTGTTAATCGCGACGGAAATACATG‑acaa > 1:735462/1‑149 (MQ=255) gAACAATCGCCAGGCTTTTGTAAACACGAACAATAAAGTGTTCCCCTTTGCAGAAAGTTTAGTTCGGAACTTCAGGCT‑ATAAAACGAATCTGAAGAACACAGCAATTTTGCGTTATCTGTTAATCGAGACTGAAATACATG‑AAAAAAAc < 1:411530/149‑1 (MQ=255) aCAATAACCAGGCTTTTGTAAAGACGAACAATAAATTTTTACCTTTTGCAGAAACTTTAGTTCGGAACTTCAGGCT‑ATAAAACGAATCTGAAGAACACAGCAATTTTGCGTTATCTGTTAATCGAGACTGAAATACATG‑AAAAAAACca < 1:11758/149‑1 (MQ=255) ccAGGCTTTTGTAAAGACGAACAATAAATTTTTACCTTTTGCAGAAACTTTAGTTCGGAACTTCAGGCT‑ATAAAACGAATATGAAGAACACAGCAATTTTACGTAATCTATTAATCGAAACTAAAGTGCATT‑AAAAAAAGAAAATTAGc > 1:364291/1‑149 (MQ=255) cAGGCTTTTGTAAAGACGAACAATAAATTTTTACCTTTTGCAGAAACTTTAGTTCGGAACTTCAGGCT‑ATAAAACGAATCTGAAGAACACAGCAATTTTGCGTTATCTGTTAATCGAGACTGAAATACATG‑AAAAAAACCACATTAGCa > 1:344738/1‑149 (MQ=255) cAGGCTTTTGTAAAGACGAACAATAAATTTTTACCTTTTGCAGAAACTTTAGTTCGGAACTTCAGGCT‑ATAAAACGAATCTGAAGAACACAGCAATTTTGCGTTATCTGTTAATCGAGACTGAAATACATG‑AAAAAAACCACATTAGCa > 2:544066/1‑149 (MQ=255) ccgttatAAAGACGAACAATAAATTATTACCATCTCCAGAAACTTTAGTTCGGAACTTCAGGCT‑ATAAAACGAATCCGAAGAACACAGCAATTTTGCGTTATCTGTTAATCGAGACTAAAATACAAA‑AAAAAAACCACATTAGCACTGa < 2:42978/143‑1 (MQ=255) cTTTTGTAAAGACGAACAATAAATTTTTACCTTTTGCAGAAACTTTAGTTCGGAACTTCAGGCT‑ATAAAACGAATCTGAAGAACACAGCAATTTTGCGTTATCTGTTAATCGAGACTGAAATACATG‑AAAAAAACCACATTAGCACTGa < 1:285392/149‑1 (MQ=255) ttttGTAAAGACGAACAATAAATTTTTACCTTTTGCAGAAACTTTAGTTCGGAACTTCAGGCT‑ATAAAACGAATCTGAAGAACACAGCAATTTTGCGTTATCTGTTAATCGAGACTGAAATACATGAAAAAAAACCACATTAGCACTGa < 1:559617/149‑1 (MQ=255) aaaGACGAACAATAAATTTTTACCTTTTGCAGAAACTTTAGTTCGGCACTTCAGGCT‑ATAACACGAATCTGAAGAACACAGCAATTTTGCGTTAACTGTTAATCGAGACTGAGATACCTG‑AAAAAAACCACATTAGCACTGAGTGCCCt > 1:433426/1‑149 (MQ=255) aGACGAACAATAAATTTTTACCTTTTGCAGAAACTTTAGTTCGGAACTTCAGGCT‑ATAAAACGAATCTGAAGAACACAGCAATTTTGCGTTATCTGTTAATCGAGACTGAAATACATG‑AAAAAAACCACATTAGCACTGAGTGCACTgg < 2:185479/149‑1 (MQ=255) gACGAACAATAAATTTTTACCTTTCGCAGAAACTTTAGTTCGGAACTTCAGGCT‑ATAAAACGAATCTGAAGAACACAGCAATTTTGCGTTATCTGTTAATCGAGACTGAAATACATG‑AAAAAAACCACATTAGCACTGAGTGCACTGGc < 1:566185/149‑1 (MQ=255) gACGAACAATAAATTTTTACCTTTCGCAGAAACTTTAGTTCGGAACTTCAGGCT‑ATAAAACGAATCTGAAGAACACAGCAATTTTGCGTTATCTGTTAATCGAGACTGAAATACATG‑AAAAAAACCACATTAGCACTGAGTGCACTGGc < 1:575885/149‑1 (MQ=255) aaCTATGAATTTTTACCTTTTGCAGAAACTTTAGTTCGGAACTTCAGGCT‑ATAAAACGAATCTGAAGAACACAGCAATTTTGCGTTATCTGTTAATCGAGACTGAAATACATG‑AAAAAAACCACATTAGCACTGAGTGCACTGGCTCTg < 1:298680/149‑1 (MQ=255) cAATAAATTTTTACCTTTAACAGAAACTTTAGTACGGAA‑ACCATGTTGCCAAAATGCATCTTAAGGACACAGCAATTTTGCGTTATCTGTTAATCGAGACTGAAATACATG‑AAAAAAACCACATTAGCACTGAGTGCACTGGCTCTggg > 1:586827/1‑147 (MQ=255) aagaaaTTTTTCCCTTTTGCAGAAACTTTAGTTCGGAACTTCAGGCT‑ATAAAACGAATCTGAAGAACACAGCAATTTTGCGTTATCTGTTAATCGAGACTGAAATACATG‑AAAAAAACCACATTAGCACTGAGTGCACTGGCTCTGAGt < 1:282121/146‑1 (MQ=255) aaTAAATTTTTACCTTTTGCAGAAACTTTAGTTCGGAACTTCAGGCT‑ATAAAACGAATCTTAAGAACACAGCAATTTTGCGTTATATGTTAATCGAGACTGAAATACATG‑ATAAAAACCACATTAGCACTGAGTGCACTGGCTCTGAGt < 1:517838/149‑1 (MQ=255) aaTTTTTACCTTTTGCAGAAACTTTAGTTCGGAACTTCAGGCT‑ATAAAACGAATCTGAAGAACACAGCAATTTTGCGTTATCTGTTACTCGAGACCGAAATACATG‑AAAAAAACCACATTAGCACTGAGTGCACTGGCTCTGAGTTTAg < 1:550977/149‑1 (MQ=255) aaTTTTTACCTTTTGCAGAAACTTTAGTTCGGAACTTCAGGCT‑ATAAAACGAATCTGAAGAACACAGCAATTTTGCGTTATCTGTTAATCGAGACTGAAATACATG‑AAAAAAACCACATTAGCACTGAGTGCACTGGCTCTGAGTTTAg > 1:777755/1‑149 (MQ=255) aaTTTTTACCTTTTGCAGAAACTTTAGTTCGGAACTTCAGGCT‑ATAAAACGAATCTAAAGAACACAGCAATTTTGCGTTATCTGTTAATCGAGACTGAAATACATG‑AAAAAAACCACATTAGCACTGAGTGCACTGGCTCTGAGTTTAg > 1:625175/1‑149 (MQ=255) tACCTTTTGCAGAAACTTTAGGTCGGAACTTCAGGCT‑ATAAAACGAATCTGAAGAACACAGCAATTTTGCGTTATCTGTTAAGCGAGACTGAAATACATG‑AAAAAAACCACATTAGCACTGAGAGCACTGGCTCTGAGTTTAGGTTTgg < 1:399591/149‑1 (MQ=255) aCCTTTTGCAGAAACTTTAGTTCGGAACTTCAGGCT‑ATAAAACGAATCTGAAGAACACAGCAATTTTGCGTTATCTGTTAATCGAGACTGAAATACATG‑AAAAAAACCACATTAGCACTGAGTGCACTGGCTCTGAGTTTAGGTTTGGc < 1:162038/149‑1 (MQ=255) | CTATGGGAATATTATTACCGTTGCCGCCTGCTGCAGGATTATATCAGCGGTATGACCGACCTCTATGCGTGGGATGAATACCGACGTCTGATGGCCGTAGAACAATAACCAGGCTTTTGTAAAGACGAACAATAAATTTTTACCTTTTGCAGAAACTTTAGTTCGGAACTTCAGGCT‑ATAAAACGAATCTGAAGAACACAGCAATTTTGCGTTATCTGTTAATCGAGACTGAAATACATG‑AAAAAAACCACATTAGCACTGAGTGCACTGGCTCTGAGTTTAGGTTTGGC > NC_000913/180647‑180936 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |