Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,793,898	Δ1 bp	coding (212/552 nt)	btuE ←	glutathione peroxidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,793,897	0	T	.	98.7%	337.2 / ‑1.8	76	coding (213/552 nt)	btuE	glutathione peroxidase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base . (36/38);  minor base A (1/0);  total (38/38)
Fisher's exact test for biased strand distribution p-value = 4.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAG‑CGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTGAGGCGACATTGACAATCAACAGCACATTACCGGCG  >  NC_000913/1793751‑1794041                                                                                                                                                    |                                                                                                                                                 gcgACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAG‑CGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCtgt                                                                                                                                                >  1:1073758/1‑147 (MQ=255)      tGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAG‑CGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAg                                                                                                                                          >  1:295599/1‑149 (MQ=255)          gTCGGCGCTGCGGCAATCAATTTTTGATACAG‑CGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAAc                                                                                                                                      <  2:964586/149‑1 (MQ=255)          gTCGGCGCTGCGGCAATCAATTTTTGAT‑CAGCCGCGTTGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAAc                                                                                                                                      >  2:303292/1‑149 (MQ=255)           tCGGCGCTGCGGCAATCAATTTTTGATACAG‑CGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACt                                                                                                                                     >  1:591799/1‑149 (MQ=255)                   gCGGCAATCAATTTTTGATACAG‑CGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTCGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCAc                                                                                                                             <  2:1031859/149‑1 (MQ=255)                     ggCAATCCAGGTTTGATACAG‑CGGAGGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACgg                                                                                                                           <  1:1094113/149‑1 (MQ=255)                      gCAATCAATTTTTGATACAG‑CGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCAAGAAACGGGTTGCACggg                                                                                                                          >  1:528369/1‑149 (MQ=255)                       caatccatTTTTGATACAG‑CGGCTGGTGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCCGACAATGGTGGCGCGGGa                                                                                                                         <  2:711207/149‑1 (MQ=255)                         atcaatTTTTGATACAG‑CGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAAt                                                                                                                       >  1:1198085/1‑149 (MQ=255)                          tcaatTTTTGATACAG‑CGAATAGCGTGCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATc                                                                                                                      <  1:253391/149‑1 (MQ=255)                            aatTTTTGATACAG‑CGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATccc                                                                                                                    >  1:680486/1‑149 (MQ=255)                            aatTTTTGATACAG‑CGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATccc                                                                                                                    >  2:550823/1‑149 (MQ=255)                              tttttGATACAG‑CGGAGGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAg                                                                                                                  <  1:840163/149‑1 (MQ=255)                                  tGATACTG‑CGGACTGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTTATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCAcc                                                                                                              <  2:594873/149‑1 (MQ=255)                                   gATACAG‑CGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCa                                                                                                             >  2:759008/1‑149 (MQ=255)                                     tACAG‑CGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATa                                                                                                           >  1:549232/1‑149 (MQ=255)                                      ataG‑CCGATCGCGTCCTGCGCCATTAACTTCAATCTTACAGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑CGTCCCAGAAACTGGTTGCACGGGAATCCCAGCACCATaa                                                                                                          <  1:1260868/147‑1 (MQ=255)                                        aG‑CGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATaaaa                                                                                                        <  1:792853/149‑1 (MQ=255)                                        aG‑CGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATaaaa                                                                                                        <  2:726746/149‑1 (MQ=255)                                         g‑CGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGG‑TCCTGTTCCAGAAACTGGTTGCACGGGAATCCCCGCACCATacacc                                                                                                       >  1:14253/1‑145 (MQ=255)                                           cGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTT‑ATGTTCCGGAAACTGGTTGCACGGGAATCCCAGCACCAGAAAAcc                                                                                                      >  2:1144382/1‑149 (MQ=255)                                             gaaggcggcCTTCGCCCTTAACTTCAATCTTACGGACCATCGGGAACGTCCCCCCCCATGTGGTGGTACAGGAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCtc                                                                                                    <  2:1130686/146‑1 (MQ=255)                                             gATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCTATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAAcccc                                                                                                    >  2:905526/1‑147 (MQ=255)                                             gATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCtc                                                                                                    <  1:1389589/149‑1 (MQ=255)                                              aTGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCGCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCtcg                                                                                                   >  2:431046/1‑149 (MQ=255)                                                ggCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCGGCACCATAAAACCtcgat                                                                                                 >  1:151830/1‑149 (MQ=255)                                                ggCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCtcgat                                                                                                 <  1:761503/149‑1 (MQ=255)                                                   ggccatCGCCATTAACGTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCtcgatcga                                                                                              <  2:753272/144‑1 (MQ=255)                                                   gTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCtcgatcga                                                                                              >  2:1110383/1‑149 (MQ=255)                                                       ttCGCCATTCACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCa                                                                                          <  1:592225/149‑1 (MQ=255)                                                        tCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAg                                                                                         >  1:473110/1‑149 (MQ=255)                                                         cGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCACGAAAGTGATTGCACGGGAATCCCAGCACCATAAAACCtcggtcgtacctgg                                                                                        >  1:38169/1‑142 (MQ=255)                                                               tAACTCCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCt                                                                                  <  2:289628/149‑1 (MQ=255)                                                                  cTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGaa                                                                               <  2:1291425/149‑1 (MQ=255)                                                                   ttCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCtacat                                                                              >  2:349254/1‑145 (MQ=255)                                                                      aaTCTTATTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAATCAGTTTTCAGCTCTTCACCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAACACCTCGATCGACCCAGGCTTTCTGAATAtt                                                                           <  2:641274/149‑1 (MQ=255)                                                                       aTCTTATTGAACATCCGTAACGTCACCCCCCATGTGGTAGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGTACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATtc                                                                          <  2:373116/149‑1 (MQ=255)                                                                            aCTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATGTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCaa                                                                     >  1:549131/1‑149 (MQ=255)                                                                              tGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACt                                                                   >  1:829354/1‑149 (MQ=255)                                                                                 aCATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCt                                                                >  1:290413/1‑149 (MQ=255)                                                                                   aTCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTTCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCa                                                              >  1:961712/1‑149 (MQ=255)                                                                                   aTCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCAGCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCCCCAACTGCTCa                                                              >  1:962127/1‑149 (MQ=255)                                                                                          aCGTCACCCCCCATGTGGTGGTACAGTAATTTTTAATCTCTTCATCGCTGCCCTGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTgcg                                                       <  2:1030314/149‑1 (MQ=255)                                                                                          aCGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAATTGCTCATATTgcg                                                       <  2:967732/149‑1 (MQ=255)                                                                                            gTCACCGCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCATGCGTTCTGAATATTCTCCAACTGCTCATATTgcggc                                                     <  2:1276022/149‑1 (MQ=255)                                                                                             tCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTgcggcg                                                    <  1:1155767/149‑1 (MQ=255)                                                                                               tccccccAGGTGGTGGTACAGGAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGtt                                                  <  2:279883/148‑1 (MQ=255)                                                                                                ccccccATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTa                                                 <  2:220758/149‑1 (MQ=255)                                                                                                 cccccATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTaa                                                <  2:945783/149‑1 (MQ=255)                                                                                                 cccccATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTaa                                                >  1:244859/1‑149 (MQ=255)                                                                                                 cccccATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCGGAATATTCTCCAACTGCTCATATTGCGGCGTTaa                                                >  1:1296/1‑149 (MQ=255)                                                                                                  ccccATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATACTGCGGCGTTAAg                                               >  1:912929/1‑149 (MQ=255)                                                                                                   cccATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAGCTGGTTGCACGGGAATCCCAGCACCATAACACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGc                                              <  2:295599/149‑1 (MQ=255)                                                                                                             ggtACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCAAGGGAATACAAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTGa                                    >  2:1264575/1‑149 (MQ=255)                                                                                                               tACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTGAgg                                  <  1:303292/149‑1 (MQ=255)                                                                                                                aCAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAATCTGGTGGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTGAGGc                                 <  2:1332277/149‑1 (MQ=255)                                                                                                                   gTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTGAGGCGAc                              >  2:426785/1‑149 (MQ=255)                                                                                                                    tAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGACTCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTGAGGCGAcc                             >  2:1209077/1‑148 (MQ=255)                                                                                                                        ttttAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGTTCGACCCAGACTTTCGGAATATTCTCCAACTGCTCCTATTGCGCCGTTAAGCCACACTTTGAGGCGACATTGa                         <  1:905526/149‑1 (MQ=255)                                                                                                                        ttttAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGGCCCAGGCTTTCTTCTTTAACTCCAACTGCTCATATTGCAGCGATAAGCCACACTTTGAGGCGACATTGa                         >  1:611929/1‑149 (MQ=255)                                                                                                                          ttAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTGAGGCGACATTGACa                       >  1:143632/1‑149 (MQ=255)                                                                                                                           tAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTGAGGCGACATTGACaa                      <  2:836523/149‑1 (MQ=255)                                                                                                                             aTCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTGAGGCGACATTGACAATc                    >  2:797223/1‑149 (MQ=255)                                                                                                                                 ctTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTGAGGCGGCATTGACAATCAAcc                >  1:1116317/1‑148 (MQ=255)                                                                                                                                  ttCATCGCTGCCCGGTTC‑TGTTCCAGAACCTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTGAGGCGACATTGACAATCAACAg               <  1:757414/149‑1 (MQ=255)                                                                                                                                  ttCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTGAGGCGACATTGACAATCAACAg               >  2:359817/1‑149 (MQ=255)                                                                                                                                  ttCATCGCTGCCCGGTGC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTGAGGCGACATTGACAATCAACAg               <  1:822749/149‑1 (MQ=255)                                                                                                                                  ttCAGCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAACCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTGAGGCGACATTGACAATCAACAg               <  2:528369/149‑1 (MQ=255)                                                                                                                                    cagcGCTGCCCGGGTC‑TGTACCATAATCTGGTTGCACGGGATGCCCAGCACCATCAACCCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACGGCTCATATGGCGGCGTTAAGCCACGCGTTGAGGCGACATTGACAATCGACGGca             <  2:1327304/146‑1 (MQ=255)                                                                                                                                    cATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTGAGGCGACATTGACAATCAACAGca             <  1:550823/149‑1 (MQ=255)                                                                                                                                     aTCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTGAGGCGACATTGACAATCAACAGcac            >  1:243724/1‑149 (MQ=255)                                                                                                                                      tCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTGAGGCGACATTGACAATCAACAGcaca           >  1:345526/1‑149 (MQ=255)                                                                                                                                         cTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTGAGGCGACATTGACAATCAACAGCACAttc        >  2:509060/1‑148 (MQ=255)                                                                                                                                           gCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTGAGGCGACATTGACAATCAACAGCACATTAcc      <  2:162303/149‑1 (MQ=255)                                                                                                                                             ccGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTGAGGCGACATTGACAATCAACAGCACATTACCgg    <  1:759008/149‑1 (MQ=255)                                                                                                                                               ggTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCCTACCGCGCGGGTAAGCCACACTTTGAGGCGACATTGACAATCAACAGCCCATTACCGGCg  >  1:1304294/1‑149 (MQ=255)                                                                                                                                                    |                                                                                                                                                 GCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAG‑CGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTGAGGCGACATTGACAATCAACAGCACATTACCGGCG  >  NC_000913/1793751‑1794041

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑