| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,399,892 | A→C | I214L (ATT→CTT) | miaA → | delta(2)‑isopentenylpyrophosphate tRNA‑adenosine transferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,399,892 | 0 | A | C | 96.6% | 278.3 / ‑5.0 | 89 | I214L (ATT→CTT) | miaA | delta(2)‑isopentenylpyrophosphate tRNA‑adenosine transferase |
| Reads supporting (aligned to +/- strand): ref base A (2/1); new base C (35/51); total (37/52) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.68e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
CACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTCATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCATTGA‑GCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTGCATACGGACTTGCCTTCCATTCGTTGCGTGGGTTATCGCCAGATGTGGTCTTACCTTGAAGGCGAAA > NC_000913/4399746‑4400036 | cacaAGTGCTTTCCCGGGCACTGGAAGTTTTTTTCATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCCtt < 2:1093198/149‑1 (MQ=255)cacaAAGGCTTTCCCGGGCACTGGAAGTTTTTTTCTTTTCGGTTCAAACTTTAACGGAACTGCCGCAACCGTCAGGAGACGCTCTACCGTATCCGGTGCCTCAGTTCGCCATCGCCCCGGCGAGCCGGGGACCGGTCCATGCACGCCtt > 2:182556/1‑149 (MQ=255) aacggCTTCCCCGGGCACGGGAGCTTGTTTTCATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGCCAGGAGGCTCTCTACCGTATCAGGTGCATCAGTACGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCCTTGa‑g < 1:1252669/146‑1 (MQ=255) tttCCCGGGCACTGGAAGTTTTTTTCATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCACCCCGGCGAGCCGTGAACTGCTCCATCAACGCCTTGA‑GCAGCGt > 1:611645/1‑149 (MQ=255) ttCCCGGGCACTGGAAGTTTTTTTCATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCCTTGA‑GCAGCGtt < 2:827027/149‑1 (MQ=255) ggCACTGGAAGTTTTTTTCATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCCTTGA‑GCAGCGTTTtcatc < 1:64716/149‑1 (MQ=255) tGGAAGTTTTTTTCATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGCGCCGTGAACTGCTCCATCAACGCCTTGA‑GCAGCGCTTtcatacgcta > 2:837165/1‑143 (MQ=255) tttttttCATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGATGTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCCTTGC‑GCAGCGTTTTCATCAGATGTTGGCt > 2:1198069/1‑149 (MQ=255) ttCATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCCTTGA‑GCAGCGTTTTCATCAGATGTTGGCTTCAgg < 1:754296/149‑1 (MQ=255) tCATGTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCCTTGA‑GCAGCGTTTTCATCAGATGTTGGCTTCAGGt < 1:236607/149‑1 (MQ=255) aTTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCGACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCCTTGA‑TCAGCGTTTTCATCAGATGTTGGCTTCAGGttt < 2:301826/149‑1 (MQ=255) tttCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCCTTGA‑GCAGCGTTTTCATCGGATGTTGGCTTCAGGtttt < 2:945781/149‑1 (MQ=255) tCGGGTAAAACTTTAGCGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCCTTGA‑GCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGa < 2:149800/149‑1 (MQ=255) tCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCCTTGA‑GCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGa < 1:1251371/149‑1 (MQ=255) cGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCCTTGA‑GCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGaa > 1:1228386/1‑149 (MQ=255) cGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCCTTGA‑GCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGaa < 1:1294289/149‑1 (MQ=255) ggTAAAACTTTAACGGAACTGACGCAAACGTCAGGCGACGCTCGACCGTATCAGGTGCATCACTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCCTTGA‑GCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGc < 2:557871/149‑1 (MQ=255) ggTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGCCGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCCTTGA‑GCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGc < 2:557589/149‑1 (MQ=255) tAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACTGTATCAGGTGCATCAGTGCGCCATCGCCCCGGCGAGCTATGAACTGCTCCATCAACGCCTTGA‑GCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAg < 2:640102/149‑1 (MQ=255) aaaaCTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCCTTGA‑GCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGa > 1:116920/1‑149 (MQ=255) aaaCTTTAACGGAACTGACGCAAACGTCAGGGGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCCTTGA‑GCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGaa < 1:32318/149‑1 (MQ=255) tAACGGAACTGACGCACACGTCAGGAGACGCTCTACCGTATCAGGTGCCTCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCCTTGA‑GCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCgg < 1:1106577/149‑1 (MQ=255) aCGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCCTTGA‑GCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGgc < 1:1272712/149‑1 (MQ=255) aCGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCCTTGA‑GCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGgc < 1:489170/149‑1 (MQ=255) aCGGAACTGACGCAAAAGTCAGGAGACGCGCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGACCCATCAACGCATTGC‑GCAGGGTTTTCATCAGATGTTGGCTTCAGGTTGTGACGCAGGAGTCCGGgc > 1:876843/1‑149 (MQ=255) gggAAGTGACGCTAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCCTTGA‑GCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGACCGGgcg > 2:926339/2‑149 (MQ=255) cGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCCTTGG‑GCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGgcg > 2:706598/1‑149 (MQ=255) ggAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCCTTGA‑GCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGgcgc < 1:1064945/149‑1 (MQ=255) ggAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTACATCAAATACAGGA‑GCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGgcgc > 1:1366833/1‑149 (MQ=255) aCGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCCTTGA‑GCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGc > 2:438963/1‑149 (MQ=255) aCGCAAACGACAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCCTTGA‑GGCGCGTTTTCATGAGATGTTGGCTTCAGGTTTTGAAGCAGAAGGCCGGGCGCTTTTTGc > 2:67384/1‑149 (MQ=255) cGCAAACGTCAGGAGACGCTCTCCCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCCTTGA‑GCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGcc < 2:400472/149‑1 (MQ=255) cGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCCTTGA‑GCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGcc < 1:117792/149‑1 (MQ=255) cGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCATTGA‑GCAGCGTTTTCATCAGATTTTTGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGcc < 1:886778/149‑1 (MQ=255) cAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCCTTGA‑GCAGCGTTTTCATCAGATGTTGGCTTCAGGCTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCg > 2:609493/1‑149 (MQ=255) aaaCGTCAGGAGACGCACTACCTTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCAACAACGCCTTGA‑GCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGACCGGGCGCTTTTTGCCCga < 1:1246649/149‑1 (MQ=255) aCGTCAGGAGACGCTCTACCGTATCAGGTGCATAAGTTCGCCATCGCCCCGGCGAGCGGTGAACTGCTCCATCAACGCCTTGA‑GCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCgagg > 2:444783/1‑149 (MQ=255) gTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGAGAGGACAATAATGCTCC‑TTTACGGAACGATGTAGCGGTTTCATCCGGTGATGGAATCAGGTGTTGAAGCAGAAGTCCGGGCGCGTTTTGCCCgaagag > 1:676735/1‑149 (MQ=255) gagaCGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGCGCCGTGAACTGCTCCATCAACGCCTTGA‑GCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTg > 1:280214/1‑149 (MQ=255) gagaCGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCCTTGA‑GCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTg > 1:220363/1‑149 (MQ=255) gagaCGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCCTTGA‑GCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTg > 2:552187/1‑149 (MQ=255) agaCGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCCTTGA‑GCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTGc < 2:588668/149‑1 (MQ=255) gccctACGGTAGCAGGTGCATCAGCTCGCCATCGCCCCGGCGAGCCGTGAACTGCCCCATCAACGCCCTGA‑GCAGCGTCTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTGCATAc < 1:1145198/146‑1 (MQ=255) ctctACCGTATCAGGTGCATCAGTTCGCCCTCGCCCCGGCGAGCCGGGAACTGCTCCATCAACGCCTTGA‑GCAGCGTTTTCATCAGATGTTGGCTTAAGGTTTTGAAGCAGAAGTCCGGGCGATTTTTGCCCGAGGAGATTCGCATAca > 1:1145799/1‑148 (MQ=255) ctACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCCTTGA‑GCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTGCATACGGa < 2:116920/149‑1 (MQ=255) tACCGTATCAGGTGCACCAGTTCGCCACCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCCTTGA‑GCAGCGTTTTCAGTAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTGCATACGGAc < 2:1356695/149‑1 (MQ=255) aCCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCCTTGA‑GCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTGCATACGGACt > 1:463954/1‑149 (MQ=255) aCCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCCTTGA‑GCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTGCATACGGACt < 1:180460/149‑1 (MQ=255) ccGTATCAGGTGCCTCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCCTTGA‑GCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTGCATACGGACtt < 2:177773/149‑1 (MQ=255) ccGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCCTTGA‑GCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTGCATACGGACtt > 1:1110090/1‑149 (MQ=255) ccGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCCTTGA‑GCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTGCATACGGACtt > 1:1110074/1‑149 (MQ=255) gTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCACCGCCTTGA‑GCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTGCATACGGACTTGc < 2:188788/149‑1 (MQ=255) tATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCCTTGA‑GCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTGCATACGGACTTGcc < 1:557133/149‑1 (MQ=255) aTCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCCTTGA‑GCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTGCATACGGACTTGCCt < 1:461033/149‑1 (MQ=255) aTCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCCTTGA‑GCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTGCATACGGACTTGCCt < 1:1254337/149‑1 (MQ=255) ggTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCCTTGA‑GCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTGCATACGGACTTGCCTTCCa < 1:328363/149‑1 (MQ=255) gCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCCTTGA‑GGAGCGTGTTCATCAGATGTGGGCTGCAGGTTTTGAAGCAGAAGTCCGGGCGCTTGTTGCCCGAGGAGATTTGCATACGGACTTGCCTTCCATTc < 1:623702/149‑1 (MQ=255) gCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCCTTGA‑GCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTGCATACGGACTTGCCTTCCATTc > 1:522523/1‑149 (MQ=255) aTCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCCTTGA‑GCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTGCATACGGACTTGCCTTCCATTCGt < 1:1198069/149‑1 (MQ=255) cAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCCTTGA‑GCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTGCATACGGACTTGCCTTCCATTCGTTg < 2:942650/149‑1 (MQ=255) cAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCCTTGA‑GCAGCGTATTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTGCATACGGACTTGCCTTCCATTCGTTg > 2:1023435/1‑149 (MQ=255) gTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCCTTGA‑GCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTGCATACGGACTTGCCTTCCATTCGTTGCg > 2:875616/1‑149 (MQ=255) ttCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCCTTGA‑GCAGCGTTTTCATCAGCTGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTGCATACGGACTTGCCTTCCATTCGTTGCGt > 2:1129040/1‑149 (MQ=255) ttCGCCATCACCCCGGCGAGCCGTGAACTGCTCCATCAACGCCTTGA‑GCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTGCATACGGACTTGCCTTCCATTCGTTGCGt < 2:611645/149‑1 (MQ=255) gCCATCGCCCCGGCGAGCCGTGAGCTGCTCCATCAACGCCGTGA‑GCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTGTTTGCCCGAGGAGATTTGCATACGGACTTGCCTTCCATTCGTTGCGTggg > 1:737056/1‑149 (MQ=255) ccATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCCTTGA‑GCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGCAGTCCGGGCGCTTTTTGCCCGAGGAGATTTGCATCCGGACTTGCCTTCCATTCGTTGCGTGGGt < 1:1110694/149‑1 (MQ=255) cATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCCTTGA‑GCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTGCATACGGACTTGCCTTCCATTCGTTGCGTGGGtt > 1:935465/1‑149 (MQ=255) aTCGCCCCGCCGAGCCGTGACCTGCTCCAACAACGCCTTGA‑GCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGTGCAACTTTGCGCACGGCCTCGCCCTCCATTCATTGCGAGCGTTa > 2:818193/1‑149 (MQ=255) cGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCCTTGA‑GCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCACGAGGAGATTTGCATACGGACTTGCCTTCCATTCGTTGCGTGGGTTATc < 1:997385/149‑1 (MQ=255) cccGGCGAGCCGTGAACTGCTGCATCAACGCCTTGA‑GCAGCGTTTTCATCAGATGCTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGGTGTGCATGCGGACTTGCCTTCCATTCGTTACGTGGGTTATCGcc > 1:985942/1‑149 (MQ=255) cccGGCGAGCCGTGAACTGCTCCATCAACGCCTAGA‑GCAGCGTTTTCATCCGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGATATTTGCATGCGGACTTGCCTTCCATTCGTTGCGTGGGTTATCGca > 2:380740/1‑148 (MQ=255) gAGCCGTGAACTGCTCCATCAACGCCTTGA‑GCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTGCATACGGACTTGCCTTCCATTCGTTGCGTGGGTTATCGCCAGAtgt < 1:609493/149‑1 (MQ=255) gCCGTGAACTGCTCCATCAACGCCTTGA‑GCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTGCATACGGACTTGCCTTCCATTCGTTGCGTGGGTTATCGCCAGATGTgg < 1:1091332/149‑1 (MQ=255) cGTGAACTGCTCCATCAACGCCTTGA‑GCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTGCATACGGACTTGCCTTCCATTCGTTGCGTGGGTTATCGCCAGATGTGGTc > 1:368853/1‑149 (MQ=255) gTGAACTGCTCCATCAACGCCTTGA‑GCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTGCATACGGACTTGCCTTCCATTCGTTGCGTGGGTTATCGCCAGATGTGGCCt > 2:1241555/1‑149 (MQ=255) aCTGCTCCATCAACGCCTTGA‑GCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTGCATACGGACTTGCCTTCCATTCGTTGCGTGGGTTATCGCCAGATGTGGTCTTAcc < 1:462018/149‑1 (MQ=255) aCTGCCCCAGCACCGCCTTGA‑GCAGCCTGTTCATCAGATGTTGGCTTCAGCTCTTGACGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTGCATACGGACTTGCCGGCCATCCGTTGCGTGGGTTATCGCCAGATGTGGTCTTAca < 2:747857/149‑2 (MQ=255) cTGCTCCATCAACGCCTTGA‑GCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTGCATACGGACTTGCCTTCCATTCGTTGCGTGGGTTATCGCCAGAtg > 2:146608/1‑138 (MQ=255) cTGCTCCATCAACGCCTTGA‑GCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTGCATACGGACTTGCCTTCCATTCGTTGCGTGGGTTATCGCCAGAtg < 1:146608/138‑1 (MQ=255) tacATCAACGCCTTGA‑GCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTGCATACGGACTTGCCTTCCATTCGTTGCGTGGGTTATCGCCAGATGTGGTCTTACCTTGaa < 1:206443/147‑1 (MQ=255) tCCATCAACGCCTTGA‑GCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTGCATACGGACTTGCCTTCCATTCGTTGCGTGGGTTATCGCCAGATGTGGTCTTACCTTGaa < 1:283031/149‑1 (MQ=255) ccATCAACGCCTTGA‑GCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTGCATACGGACTTGCCTTCCATTCGTTGCGTGGGTTATCGCCAGATGTGGTCTTACCTTGAAg < 2:1228386/149‑1 (MQ=255) ccATCAACGCCTTGA‑GCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTGCATACGGACTTGCCTTCCATTCGTTGCGTGGGTTATCGCCAGATGTGGTCTTACCTTGAAg < 2:1139352/149‑1 (MQ=255) cATCAACGCCTTGA‑GCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTGCATACGGACTTGCCTTCCATTCGTTGCGTGGGTTATCGCCAGATGTGGTCTTCCCTTGAAgg > 2:535848/1‑149 (MQ=255) cAACGCCTTGA‑GCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTGCATACTGACTTGCCTTCCATTCGTTGCGTGGGTTATCGCCAGATGTGGTCTTACCTTGAAGGCGa < 2:1029913/149‑1 (MQ=255) aCGCCTTGA‑GCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTGCATACGGACTTGCCTTCCATTCGTTGCGTGGGTTATCGCCAGATGTGGTCtt < 2:608054/134‑1 (MQ=255) aCGCCTTGA‑GCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTGCATACGGACTTGCCTTCCATTCGTTGCGTGGGTTATCGCCAGATGTGGTCtt > 1:608054/1‑134 (MQ=255) aCGCCTTGA‑GCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTGCATACGGACTTGCCTTCCATTCGTTGCGTGGGTTATCGCCAGATGTGGTCTTACCTTGAAGGCGaaa > 1:710617/1‑149 (MQ=255) aCGCCTTGA‑GCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTGCATACGGACTTGCCTTCCATTCGTTGCGTGGGTTATCGCCAGATGTGGTCTTACCTTGAAGGCGaaa < 1:294058/149‑1 (MQ=255) | CACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTCATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCATTGA‑GCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTTTTTGCCCGAGGAGATTTGCATACGGACTTGCCTTCCATTCGTTGCGTGGGTTATCGCCAGATGTGGTCTTACCTTGAAGGCGAAA > NC_000913/4399746‑4400036 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |