Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,045,099	Δ1 bp	14.3%	coding (162/7077 nt)	yeeJ →	putative adhesin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,045,099	0	A	.	14.3%	75.1 / 8.8	28	coding (162/7077 nt)	yeeJ	putative adhesin
Reads supporting (aligned to +/- strand):  ref base A (13/11);  new base . (2/2);  total (15/13)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.43e-01

AGAGAAGTGGAGAAGAAATAAATGACCGACAAATATTATGCGGGATGGGAATTAAACTACGCCGCTTAACTGCGGGTATCTGTCTGATAACTCAACTTGCGTTCCCTATGGCTGCGGCAGCACAAGGTGTGGTAAACGCCGCAACCCAACAACCAGTTCCTGCACAAATTGCCATTGCAAATGCCAATACGGTGCCCTACACCCTTGGAGCGTTGGAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGC  >  NC_000913/2044951‑2045245                                                                                                                                                     |                                                                                                                                                   agagaagTGGAGAAGAAATAAATGACCGACAAATATTATGCGGGATGGGAATTAAACTACGCCGCTTAACTGCGGGTATCTGTCTGATAACTCAACTTGCGTTCCCTATGGCTGCGGCAGCACAAGGTGTGGTAAACGCCGCAACCcaa                                                                                                                                                    <  2:647589/149‑1 (MQ=255)             aagaaATAAATGACCGACAAATATTATGCGGGATGGGAATTAAACTACGCCGCTTAACTGCGGGTATCTGTCTGATAACTCAACTTGCGTTCCCTATGGCTGCGGCAGCACAAGGTGTGGTAAACGCCGCAACCC‑‑CAACCAGTTCCTGc                                                                                                                                      >  1:751983/1‑149 (MQ=255)             aagaaATAAATGACCGACAAATATTATGCGGGATGGGAATTAAACTACGCCGCGTAACTGCGGGTATATGTCTGATGACTGAACTTGCGTTGCCTATGGCTGCGGCAGCACACGGTGTGGTAAACGCCGCAACCC‑‑CAACCAGGGCCTGc                                                                                                                                      >  1:752016/1‑149 (MQ=255)                                aaTATTATGCGGGATGGGAATTAAACTACGCCGCTTAACTGCGGGTATCTGTCTGATAACTCAACTTGCGTTCCCTATGGCTGCGGCAGCACAAGGTGTGGTAAACGCCGCAACCCAACAACCAGTTCCTGCACAAATTGCCATTGCaa                                                                                                                     >  1:270438/1‑149 (MQ=255)                                  tattatGCGGGATGGGAATTAAACTACGCCGCTTAACTGCGGGTATCTGTCTGATAACTCAACTTGCGTTCCCTATGGCTGCGGCAGCACAAGGTGTGGTAAACGCCGCAACCCAACAACCAGTTCCTGCACAAATTGCCATTGCAAAt                                                                                                                   >  1:286850/1‑149 (MQ=255)                                  tattatGCGGGATGGGAATTAAACTACGCCGCTTAACTGCGGGTATCTGTCTGATAACTCAACTTGCGTTCCCTATGGCTGCGGCACCACAAGGTGTGGTAAACGCCGCAACCCAACAACCAGTTCCTGCACAAATTGCCATTGCAAAt                                                                                                                   <  2:689035/149‑1 (MQ=255)                                            gATGGGAATTAAACTACGCCGCTTAACTGCGGGTATCTGTCTGATAACTCAACTTGCGTTCCCTATGGCTGCGGCAGCACAAGGTGTGGTAAACGCCGCAACCCAACAACCAGTTCCTGCACAAATTGCCATTGCAAATGCCAATACgg                                                                                                         >  2:113202/1‑149 (MQ=255)                                              tGGGAATTAAACTACGCCGCTTAACTGCGGGTATCTGTCTGATAACTCAACTTGCGTTCCCTATGGCTGCGGCAGCACAAGGTGTGGTAAACGCCGCAACCCAACAACCAGTTCCTGCACAAAATGCCATTGCAACTGCCAATACGGTg                                                                                                       >  1:242425/1‑149 (MQ=255)                                                 gAATTAAACTACGCCGCTTAACTGCGGGTATCTGTCTGATAACTCAACTTGCGTTCCCTATGGCTGCGGCAGCACAAGGTGTGGTAAACGCCGCAACCCAACAACCAGTTCCTGCACAAATTGCCATTGCACATGCCAATACGGTGccc                                                                                                    >  2:86867/1‑149 (MQ=255)                                                  aaTTAAACTACGCCGCTTAACTGCGGGTATCTGTCTGATAACTCAACTTGCGTTCCCTATGGCTGCGGCAGCACAAGGTGTGGTAAACGCCGCAACCCAACAACCAGTTCCTGCCCAAATTGCCATTGCAAATGCCAATACGGTGCCCt                                                                                                   >  1:852310/1‑149 (MQ=255)                                                          tACCCCGCTTAACTGCGGGTATCTGTCTGATAACTCAACTTGCGTTCCCTATGGCTGCGGCAGCACAAGGTGTGGTAAACGCCGCAACCCAACAACCAGTTCCTGCACAAATTGCCATTGCAAATGCCAATACGGTGCCCTACACCCtt                                                                                           <  1:52008/149‑1 (MQ=255)                                                              ccgcTTAACTGCGGGTATCTGTCTGATAACTCAACTTGCGTTCCCTATGGCTGCGGCAGCACAAGGTGTGGTAAACGCCGCAACCCAACAACCAGTTCCTGCACAAATTGCCATTGCAAATGCCAATACGGTGCCCTACACCCTTGGAg                                                                                       <  1:209121/149‑1 (MQ=255)                                                              ccgcTTAACTGCGGGTATCTGTCTGATAACTCAACTTGCGTTCCCTATGGCTGCGGCAGCACAAGGTGTGGTAAACGCCGCAACCCAACAACCAGTTCCTGCACAAATTGCCATTGCAAACGCCAATACGGTGCCCTACACCCTTGGAg                                                                                       <  1:515789/149‑1 (MQ=255)                                                                      cTGCGGGTATCTGTCTGATAACTCAACTTGCGTTCCCTATGGCTGCGGCAGCACAAGGTGTGGTAAACGCCGCAACCCAACAACCAGTTCCTGCACAAATTGCCATTGCAAATGCCAATACGGTGCCCTACACCCTTGGAGCGTTGGaa                                                                               >  1:553459/1‑149 (MQ=255)                                                                      cTGCGGGTATCTGTCTGATAACTCAACTTGCGTTCCCTATGGCTGCGGCAGCACAAGGTGTGGTAAACGCCGCAACCCAACAACCAGTTCCTGCACAAATTGCCATTGCAAATGCCAATACGGTGCCCTACACCCTTGGAGCGTTGGaa                                                                               <  1:5197/149‑1 (MQ=255)                                                                             tATCTGTCTGATAACTCAACTTGCGTTCCCTATGGCTGCGGCAGCACAAGGTGTGGTAAACGCCGCAACCC‑‑CAACCAGTTCCTGCACAAATTGCCATTGCAAATGCCAATACGGTGCCCTACACCCTTGGAGCGTTGGAATCGGCCCaa                                                                      <  2:751983/149‑1 (MQ=255)                                                                             tATCTGTCAGATAACTCAACTTGCGTTCCCTATGGCTGCGGCAGCACAAGGTGTGGTAAACGCCGCAACCC‑‑CAACCAGTTCCTGCACAAATTGCCATTGCAAATGCCAATACGGTGCCCTACACCCTTGGAGCGTTGGAATCGGCCCaa                                                                      <  2:752016/149‑1 (MQ=255)                                                                                 tgtctgATAACTCAACTTGCGTTCCCTATGGCTGCGGCAGCACAAGGTGTGGTAAACGCCGCAACCCAACAACCAGTTCCTGCACAAATTGCCATTGCAAATGCCAATACGGTGCCCTACACCCTTGGAGCGTTGGAATCGGCCCAAAg                                                                    >  1:315601/1‑149 (MQ=255)                                                                                 tgtctgATAACTCAACTTGCGTTCCCTATGGCTGCGGCAGCACAAGGTGTGGTAAACGCCGCAACCCAACAACCAGTTCCTGCACAAATTGCCATTGCAAATGCCAATACGGTGCCCTACACACTTGGAGCGTTGGAATGGGCCCAAAg                                                                    >  2:122629/1‑149 (MQ=255)                                                                                       aTAACTCAACTTGCGTTCCCTATGGCTGCGGCAGCACAAGGTGTGGTAAACGCCGCAACCCAACAACCAGTTCCTGCACAAATTGCCATTGCAAATGCCAATACGGTGCCCTACACCCTTGGAGCGTTGGAATCGGCCCAAAGCGTTGc                                                              <  1:365053/149‑1 (MQ=255)                                                                                           cctagcttgcttgCCCGAGGGCTGCGGCAGCACCAGGGGTGGTAAACGCCGCAACCAAACAACCAGTTCCTGCACAACTTGCCATACCAAATGCCACTCCGGTGCCCTACCCCCTTGGAGCGTTGGAATCGGCCCAAAGCGTTGCCGaa                                                          <  1:303757/144‑1 (MQ=255)                                                                                                 ttGCGTTCCCTATGGCTGCGGCAGCACAAGGTGTGGTAAACGCCGCAACCCAACAACCAGTTCCTGCACAAATTGCCATTGCAAATGCCAATACGGTGCCCTACACCCTTGGAGCGTTGGAATCGGCCCAAAGCGTTGCCGAACGTTTc                                                    <  1:334749/149‑1 (MQ=255)                                                                                                      ttCCCTATGGCTGCGGCAGCACAAGGTGTGGTAAACGCCGCAACCCAACAACCAGTTCCTGCACAAATTGCCATTGCAAATGCCAAGACGGTGCCCTACACCCTTGGAGCGTTGGAATCGGCCCAAAGCGGCGCCGGACGTTTCGGtgt                                               >  1:106131/1‑147 (MQ=255)                                                                                                            aTGGCGGCGGCAGCACAAGGTGTGGTAAACGCCGCAACCCAACAACCAGTTCCTGCACAAATTGCCATTGCAAATGCCAATACGGTGCCCTACACCCTTGGAGCGTTGGAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGt                                         <  2:303265/149‑1 (MQ=255)                                                                                                              ggCTCCCGCAGCACAAGGCGTGGGAGACGCCGCAACCCAACAACCAGTTCCGGCATAAATTGCCATTGCAAATGCCAATACGGTGCCCTACACCCTTGGAGGGTTTGAATCGGCCGAAAGCGTTGCCGAACGTTTCGGTATTTCGGTgg                                       <  2:83940/149‑1 (MQ=255)                                                                                                                        gcaCAAGGTGTGGTAAACGCCGCAACCCAACAACCAGTTCCTGCACAAATTGCCATTGCAAATGCCAATACGGTGCCCTACACCCTTGGAGCGTTGGAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACg                             <  1:488462/149‑1 (MQ=255)                                                                                                                                  tgGTAAACGCCGCAACCCAACAACCAGTTCCTGCACAAATTGCCATTGCAAATGCCAATACGGTGCCCTACACCCTTGGAGCGTTGGAATCGGCCCAAAGCCTAACCGGACTGTTGAGTATTTCGGCGGCTGAGTTACGCAAACTCAAc                   >  1:583093/1‑149 (MQ=255)                                                                                                                                    gTAAACGCCGCAACCCAACAACCAGTTCCAGCACAAATTGCCATTGCAAATGCCAATACGGTGCCCTACACCCTTGGAGCGTTGGAATCGGCCCAAAGCGTTGCCGAACGGTTCGGTATTTCGGTGGCTGAGTTACGCAAACACAACCa                 >  1:521763/1‑149 (MQ=255)                                                                                                                                         cgccgcAACCCAACAACCAGTTCCTGCACAAATTGCCATTGCAAATGCCAATACGGTGCCCTACACCCTTGGAGCGTTGGAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAAcccggatc            >  2:344307/1‑143 (MQ=255)                                                                                                                                                   caacaaCCAGTTCCTGCACAAATTGCCATTGCAAATGCCAATACGGTGCCCTACACCCTTGGAGCGTTGGAATCGGCCCAAAGCGTTGCCGCACGTTTCGGTATTTCGGCGGCTGAGTTACGCAAACGCAACCAGATTCCGACGTTGGc  >  2:507655/1‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                                   AGAGAAGTGGAGAAGAAATAAATGACCGACAAATATTATGCGGGATGGGAATTAAACTACGCCGCTTAACTGCGGGTATCTGTCTGATAACTCAACTTGCGTTCCCTATGGCTGCGGCAGCACAAGGTGTGGTAAACGCCGCAACCCAACAACCAGTTCCTGCACAAATTGCCATTGCAAATGCCAATACGGTGCCCTACACCCTTGGAGCGTTGGAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGC  >  NC_000913/2044951‑2045245

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑