| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,093,040 | G→A | 100% | A215T (GCT→ACT) | hisC → | histidinol‑phosphate aminotransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,093,040 | 0 | G | A | 96.2% | 83.8 / ‑5.9 | 33 | A215T (GCT→ACT) | hisC | histidinol‑phosphate aminotransferase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (11/19); minor base T (0/2); total (11/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.34e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GATTTTCGCACCCTGCTGGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGC‑ACCTGGCTATTTTACGCACACTGTCGAAAGCTTTTGCTCTGGCGGGGCTTCGTTGCGG‑ATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGCCCACAGGGAATCGT > NC_000913/2092896‑2093185 | ggctttCGCACCCTGCGGGAGTTAACCCGCGGTAAGGCTATTGTGGTTGCCGATGAAGCCTCTAGCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGC‑ACCTGGCTATTTTACGCACACTGTCGAAAACttt < 2:208841/146‑1 (MQ=255) aTTTTCGTCCCCGGTTGTAGTTATCTCGCGGTATGGCGTTTGCGTTGGCGGAAGAGGCCGTTGTCGTCATTTGCACGAGGTGATCGCTGTCTGGCTGGCTGGCGGAATATCCGC‑ACGTGGCTATTTTACGCACACTGTTGAAAACtttt < 2:641367/149‑1 (MQ=255) gCACCCTGCTGGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGC‑ACCTGCCTATTTTACGCACACTGTCGAAAACTTTTGCTCTg < 2:535263/149‑1 (MQ=255) ccTGCTGGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGC‑ACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCgg > 1:799150/1‑149 (MQ=255) tgctgGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGC‑ACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCgggg < 1:30054/149‑1 (MQ=255) cccGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGC‑ACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGAGGGGCTTCGTTGCGG‑a > 1:306452/1‑149 (MQ=255) gcgGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGC‑ACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGG‑Attt < 1:668134/149‑1 (MQ=255) aaGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGC‑ACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGG‑ATTTACGCt < 1:400591/149‑1 (MQ=255) gCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGC‑ACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGG‑ATTTAGGCTGGc > 2:740192/1‑149 (MQ=255) gCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGC‑ACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGAGG‑ATTTACGCTTGCAAACGAAGAAGt > 1:296449/1‑149 (MQ=255) aTGAAGCCTAGATCGAGTTTTGCCCGAAGGCACCGCTGGCTGGCTGGCTGGCGGAATATCCGC‑ACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGG‑ATTTACGCTGGCAAACGAAGAAGtcatc < 1:213921/149‑1 (MQ=255) tGAAGCCTATACCGAGTTTTGCCCGCAGGCCTCGCTGGCTCGCTGGCTGGCGGAATATCCGCAAACCGAAGATGCT‑CGCGCAATATGAGAATGGTGGGCTCTGGCGGGGCTTCGTTGCGG‑ATTTACGCTGGCAAACGAAGAAGtcatca < 1:843480/149‑1 (MQ=255) cTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGC‑ACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGG‑ATTTACGCTGGCAAACGAAGAAGTCATCAACctgc < 1:495790/149‑1 (MQ=255) tatCGAGTCTTGCCCGCAGGCATCGCTGGCTGGCGGGCTGGCGGAATATCCGC‑ACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGG‑ATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCtga < 2:384892/149‑1 (MQ=255) tacctaGTTTCGCCCGCAGTCCTCGCTGGCTGGCTGGCTGGCGGAATATCCGC‑ACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGG‑ATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCtga < 2:455431/144‑1 (MQ=255) cGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGC‑ACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGG‑ATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCtgatga > 1:648507/1‑149 (MQ=255) aGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCCGGCGGAAGATCCGG‑ACCTGGCTATTTTACGAACACTGTCGAAAACTTCTGCTCGGGCGGCGCTTCCTTGCGG‑ATTTACGCTGGCAAACCAAGAAGTCATCAACCTGCTGATGaaa < 2:423943/149‑1 (MQ=255) aGTTGTGCCCCGAGGGATCCGTGGCTGTCTTGCTGGCGGAATATCCCT‑ACCTGGCTATTTTACGCACACTGTCGAAATCTTTTGCTCTGGCGGGGCTTCGTTGCGG‑ATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGaaa < 1:27310/149‑1 (MQ=255) cccGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGC‑ACCTGGCTATTTTAAGAACGGCAGCGTAAACTTTTGCAAGGGCGGGGCTTCGTTGCGG‑ATTTGGGGTGGCAGACGAAGAAGTCATCAACCTTCTGATGAAAGTGAtgg > 1:843410/1‑147 (MQ=255) ccGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGC‑ACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGG‑ATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGGGATCGc > 2:677289/1‑149 (MQ=255) ccGCAGGCATCGCCGGCTGGCTGGCTGGCGGAATATCCGC‑ACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCCTCGTTGCGG‑ATTTACGCTGGCAAACGCAGAAGTCAACAACCCGCTGAAGAAAGTGaaccc > 2:666973/1‑145 (MQ=255) cAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGC‑ACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGAGGGTCTGTTTTGCGG‑ATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGCAAGTGATCGcccc < 1:586769/149‑1 (MQ=255) tggctggctggctggcGGAATATCCGC‑ACCTGGCTATTTTACGCACACTGTCGCAAACTTTTGCTCTGGCGGGGCTTCGTTGCGG‑GTTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGCTGAACGTGATCGCCCCCTACCCGccc > 1:211164/1‑147 (MQ=255) gctggctggcGGAATATCCGC‑ACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGG‑ATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACg < 2:648507/149‑1 (MQ=255) ggctggcGGAATATCCGC‑ACCTGGCTATTTCCCGCCCCCTGTCGAGAACTTTTGCTCTGGCGGGGCTTCGTTGCGG‑ATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCg < 1:661906/149‑1 (MQ=255) ctggcGGAATAGCCGC‑ACCTGGCTATTTTCCGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGG‑ATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGt < 2:702620/149‑1 (MQ=255) tggcGGAATATCCGC‑ACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGG‑CTTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGtt > 1:71529/1‑149 (MQ=255) gcGGACTATGCGC‑ACCTGGCTATTTTACGCACACTGTCGAAAACTTGTGCTCTGGCGGGGCTTCGTTGCGG‑ATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGc < 2:261613/149‑1 (MQ=255) tCCGC‑ACCTGGCTATTTTACGCACACTGGCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGG‑ATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTg < 2:840150/149‑1 (MQ=255) ccTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGG‑ATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGccc < 1:610237/149‑1 (MQ=255) tGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGG‑ATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAg > 1:762470/1‑149 (MQ=255) tcgtgtACGCACATTGTCGAAAACTTTTGCTGTGGCGGGGCTTCGTTGCGG‑ATTGACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCGCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGt < 2:814268/144‑1 (MQ=255) ttttACGCACACTGTCAACACGGTTTCCGTTGGCGGGG‑ATCAGGGCGGCATCTACGCGGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTa < 1:620139/149‑1 (MQ=255) aaaaCTTTTGCTCTGGCGGGGCTTCGTTGCGG‑ATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGCCCACAGGGAAtcgt < 2:782488/149‑1 (MQ=255) | GATTTTCGCACCCTGCTGGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGC‑ACCTGGCTATTTTACGCACACTGTCGAAAGCTTTTGCTCTGGCGGGGCTTCGTTGCGG‑ATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGCCCACAGGGAATCGT > NC_000913/2092896‑2093185 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |