New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 1572380	38 (0.520)	4 (0.060)	4/266	NT	10.0%	intergenic (‑335/+27)	gadB/pqqL	glutamate decarboxylase B, PLP‑dependent/putative periplasmic M16 family zinc metalloendopeptidase
	?	NC_000913	= 1572395	35 (0.500)					intergenic (‑350/+12)	gadB/pqqL	glutamate decarboxylase B, PLP‑dependent/putative periplasmic M16 family zinc metalloendopeptidase

GCGCATTCTGTGTCTCCTGAATTATCACGTAAAAATCAGACCTTAAAATATCACTATTAGTACTTGATTATTATTTTGAACGCATTTATAAAATTATTACATAAAAATAGCGAATATTGCTAAAATCCCCGCCAACG‑ATGTGTTGACGGGGCTGTTATTATTTTGGCAATAATACTCCGGTATAAGTATTTACCGGATGAGAAAGATATTGTTTAACGGCAGTGTTAACATTCTCTACCGTCATTTGTTTCAACAATTGCTCCTGCTCAGTCCATGCTGCAGGATCGTCAT  >  NC_000913/1572250‑1572540                                                                                                                                                   |                                                                                                                                                  gcgcATTCTGTGTCTCCTGAATTATCACGTAAAAATCAGACCTTAAAATATCACTATTAGTACTTGATTATTATTTTGAACGCATTTATAAAATTATTACATAAAAATAGCGAATATTGCTAAAATCCCCGCCAACG‑ATGTGTTGAcgg                                                                                                                                                >  2:1067378‑M2/1‑146 (MQ=255) gcgcATTCTGTGTCTCCTGAATTATCACGTAAAAATCAGACCTTAAAATATCACTATTAGTACTTGATTATTATTTTGAACGCATTTATAAAATTATTACATAAAAATAGCGAATATTGCTAAAATCCCCGCCAACG‑ATGTGTTGAcgg                                                                                                                                                >  2:993648‑M2/1‑146 (MQ=255)  cgcATTCTGTGTCTCCTGAATTATCACGTAAAAATCAGACCTTAAAATATCACTATTAGTACTTGATTATTATTTTGAACGCAGTGATAAAATTATTACATAAAAATCGCGAATATTGCTAAAATCCCCGCCAACG‑ATGTGTTGAcggg                                                                                                                                               >  1:38562‑M2/1‑145 (MQ=255)     aaaaagtgtCTCCTGACTTAAGACCGAAAAATCAGACCTTAAAATATCACTATTAGTACTTGATTATTATTTTGAACGCATTTATAAAATTATTACATAAAAATAGCGAATATTGCTAAAATCCCCGCCAACG‑ATGTGTTGACGGGGCt                                                                                                                                            >  2:608288/6‑149 (MQ=255)      ttCTGTGTCTCCTGAATTATCACGTAAAAATCAGACCTTAAAATATCACTATTAGTACTTGATTATTATTTTGAACGCATTTATAAAATTATTACATAAAAATAGCGAATATTGCTAAAATCCCCGCCAACG‑ATGTGTTGACGGGGCTg                                                                                                                                           <  1:482825/149‑1 (MQ=255)              ccccTGAATTATCACGTAAAAATCAGACCTTAAAATATCACTATTAGTACTTGATTATTATTTTGAACGCATTTATAAAATTATTACATAAAAATAGCGAATATTGCTAAAATCCCCGCCAACG‑ATGTGTTGACGGGGCTGTTattatt                                                                                                                                   <  1:478930/147‑1 (MQ=255)               tcCTGAATTATCACGTAAAAATCAGACCTTAAAATATCACTATTAGTACTTGATTATTATTTTGAACGCATTTATAAAATTATTACATAAAAATAGCGAATATTGCTAAAATCACCGCCAACG‑ATGTGTTGACGGGGCTGTTATTAttt                                                                                                                                  >  1:856777/1‑149 (MQ=255)                ccTGAATTATCACGTAAAAATCAGACCTTAAAATATCACTATTAGTACTTGATTATTATTTTGAACGCATTTATAAAATTATTACATAAAAATAGCGAATATTGCTAAAATCCCCGCCAACG‑ATGTGTTGACGGGGCTGTTATTAtttt                                                                                                                                 >  2:941714/1‑149 (MQ=255)                  tGAATTATCACGTAAAAATCAGACCTTAAAATATCACTATTAGTACTTGATTATTATTTTGAACGCATTTATAAAATTATTACATAAAAATAGCGAATATTGCTAAAATCCCCGCCAACG‑ACGTGTTGAcggggattttagcaatattc                                                                                                                               <  1:902312‑M2/149‑21 (MQ=255)                   gAATTATCATGTAAAAATCAGACCTTAAAATATCACTATTAGTACTTGATTATTATTTTGAACGCATTTATAAAATTATTACATAAAAATAGCGAATATTGCTAAAATCCCCGTCAACACATC‑GTTGGtggggattttagcaatattcg                                                                                                                              >  2:1033380‑M2/1‑128 (MQ=255)                     aTTATCCTGTAAAAATCTTACCTTCAAATATCACTATTAGTACTTGATTATTATTTTGAACGCATTTATACAATTATTACATAAAAATAGCGAATATTTCTAAAATCCCCGCCAACG‑ATGTGTTGACGGGGCTGTTATTATTTTGGCaa                                                                                                                            <  2:1168400/149‑1 (MQ=255)                               aaaaaCCAAACTTTAAAATATCACTAATAGTACTTGATTATTATTTTGAACGCATTTATAAAATTATTACATACAAATAGCGAATATTGCTAAAATCCCCGCATGCG‑AGTTGTTGACGGGACTGTTATTATTTTGGCAATAATACTCCg                                                                                                                  <  1:260572/149‑1 (MQ=255)                                         ccTTAAAATATCACTATTAGTACTTGATTATTATTTTGAACGCATTTATAAAATTATTACATAAAAATAGCGAATATTGCTAAAATCCCCGCCAACG‑ATGTGTTGACGGGGCTGTTATTATTTTGACAATAATACTCCGGTATAAGTAt                                                                                                        <  2:881081/149‑1 (MQ=255)                                            tAAAATATCACTATTAGTACTTGATTATGATTTTGAACGCATTTATAAAATTATTACATAAAAATAGCGAATATTGCTAAAATCCCCGCCAACG‑ATGTGTTGACGGGGCTGGTATTATTTTGGCAATAATACTCCGGTATAAGTATTTa                                                                                                     >  1:848125/1‑149 (MQ=255)                                                    cACTATTAGTACTTGATTATTATTTTGAACGCATTTATAAAATTATTACATAAAAATAGTGAATATTGCTAAAATCCCCGCCAACG‑ATGTGTTGACGGGGCTGTTATTATTTTGGCAATAATACTCCGGTATAAGTATTTACCGGATga                                                                                             <  1:674734/149‑1 (MQ=255)                                                      cTATTAGTACTTGATTATTATTTTGAACGCATTTATAAAATTATTACATAAAAATAGCGAATATTGCTAAAATCCCCGCCAACG‑ATGTGTTGACGGGGCTGTTATTATTTTGGCAATAATACTCCGGTATAAGTATTTACCGGATgaga                                                                                           <  1:1031080/149‑1 (MQ=255)                                                      cTATTAGTACTTGATTATTATTTTGAACGCATTTATAAAATTATTACATAAAAATAGCGAATATTGCTAAAATCCCCGCCAACG‑ATGTGTTGACGGGGCTGTTATTATTTTGGCAATAATACTCCGGTATAAGTATTTACCGGATgaga                                                                                           <  2:1229481/149‑1 (MQ=255)                                                          tAGTACTTGATTATTATTTTGAACGCATTTATAAAATTATTACATAAAAATAGCGAATATTGCTAAAATCCCCGCCAACG‑ATGTGTTGACGGGGCTGTTATTATTTTGGCAATAATACTCCGGTATAAGTATTTACCGGATGAGAAAGa                                                                                       >  2:562153/1‑149 (MQ=255)                                                             tACTTGATTATTATTGTGAACCCATTTATAAAAGTATTACATAAAAATAGCGAATATTGCTAAAATCCCCGCCAACG‑ATGTGTTGACGGGGCTGTTATTATTTTGGCAATAATATTCCGGTATAAGTATTTACCGGATGAGAAAGatat                                                                                    <  2:302718/149‑1 (MQ=255)                                                             tACTTGATTATTATCTTGAACGCATTTATAAAATTATTACATAAAAATAGCGAATATTGCTAAAATCCCCGCCAACG‑ATGTGTTGACGGGGCTGTTATTATTTTGGCAATAATACTCCGGTATAAGTATTTACCGGATGAGAAAGatat                                                                                    <  1:832796/149‑1 (MQ=255)                                                               cTTGATTATTATTTTGAACGCATTTATAAAATTATTACATAAAAATAGCGAATATTGCTAAAATCCCCGCCAACT‑AGGTTTTGAACGGGCCCTGTTCATTGGGGCAATAATACGCCGGGATAAGTATTTACCGGTTGAAAAAAatatgg                                                                                  >  2:275968/1‑147 (MQ=255)                                                               cTTGATTATTATTTTGAACGCATTTATAAAATTATTACATAAAAATAGCGAATATTGCTAAAATCCCCGCCAACG‑ATGTGTTGACGGGGCTGTTATTATTTTGGCAATAATACTCCGGTATAAGTATTTACCGGATGAGAAAGATATTg                                                                                  >  1:1243932/1‑149 (MQ=255)                                                               cTTGATTATTATTTTGAACGCATTTATAAAATTATTACATAAAAATAGCGAATATTGCTAAAATCCCCGCCAACG‑ATGTGTTGACGGGGCTGTTATTATTTTGGCAATAATACTCCGGTATAAGTATTTACCGGATGAGAAAGATATTg                                                                                  <  1:334372/149‑1 (MQ=255)                                                                        tattTTGAACGCAATTATAAAATTATTACATAAAAATAGCGAATATTGCTAAAATCCCCGTCAACA‑CATCGTTGGcggggattttagcaatattcgctatttttatgtaataattttataaatgcgttcaaaataataatcaagtacta                                                                         <  2:902312‑M2/149‑75 (MQ=255)                                                                         attTTGAACGCATTTATAAAATTATTACATAAAAATAGCGAATATTGCTAAAATCCCCGCCAACG‑ATGTGTTGACGGGGCTGTTATTATTTTGGCAATAATACTCCGGTATAAGTATTTACCGGATGAGAAAGATATTGTTTAACGGCa                                                                        <  2:1040051/149‑1 (MQ=255)                                                                           tttGAACGCATTTATAAAATTATTACATAAAAATAGCGAATATTGCTAAAATCCCAGCCAACG‑ATGTGTTGACGGGGCTGTTATTATTTTGGCAATAATACTCCGGTATAAGTATTTACCGGATGAGAAAGATATTGTTTAACGGCGgt                                                                      >  2:831758/1‑149 (MQ=255)                                                                            ttGAACGCATTTATAAAATTATTACATAAAAATAGCGAATATTGCTAAAATCCCCGCCAACG‑ATGTGTTGACGGGGCTGTTATTATTTTGGCAATAATACTCCGGTATAAGTATTTACCGGATGAGAAAGATATTGTTTAACGGCAgtg                                                                     <  2:26675/149‑1 (MQ=255)                                                                            ttGAACGCATTTATAAAATTATTACATAAAAATAGCGAATATTGCTAAAATCCCCGCCAACG‑ATGTGTTGACGGGGCTGTTATTATTTTGGCAATAATACTCCGGTATAAGTATTTACCGGATGAGAAAGATATTGTTTAACGGCAgtg                                                                     >  2:999595/1‑149 (MQ=255)                                                                                    aTTTATAAAATTATTACATAAAAATAGCGAATATTGCTAAAATCCCCGCCAACG‑ATGTGTTGACGGGGCTGTTATTATTTTGGCAATAATACTCCGGTATAAGTATTTACCGGATGAGAAAGATATTGTTTAACGGCAGTGTTAACAtt                                                             <  1:183016/149‑1 (MQ=255)                                                                                          aaaaTTATTACATAAAAATAGCGAATATTGCTAAAATCCCCGCCAACG‑ATGTGTTGACGGGGCTGTTATTATTTTGGCAATAATACTCCGGTATAAGTATTTACCGGATGAGAAAGATATTGTTTAACGGCAGTGTTAACATTCTCTAc                                                       <  1:608288/149‑1 (MQ=255)                                                                                           aaaTTATTACATAAAAATAGCGAATATTGCTAAAATCCCCGCCAACG‑ATGTGTTGACGGGGCTGTTATTATTTTGGCAATAATACTCCGGTATAAGTATTTACCGGATGAGAAAGATATTGTTTAACGGCAGTGTTAACATTCTCTAcc                                                      >  2:708324/1‑149 (MQ=255)                                                                                               tattaCATAAAAATAGCGAATATTGCTAAAATCCCCGCCAACG‑ATGTGTTGACGGGGCTGTTATTATTTTGGCAATAATACTCCGGTATAAGTATTTACCGGATGAGAAAGATATTGTTTAACGGCAGTGTTAACATTCTCTACCGTCa                                                  >  1:17769/1‑149 (MQ=255)                                                                                                 ttaCATAAAAATAGCGAATATTGCTAAAATCCCCGCCAACG‑ATGTGTTGACGGGGCTGTTATTATTTTGGCAATAATACTCCGGTATAAGTATTTACCGGATGAGAAAGATATTGTTTAACGGCAGTGTTAACCTTCTCTACCGTCAtt                                                >  1:120094/1‑149 (MQ=255)                                                                                                      tAAGAATAGCGAATATTGCTAAAATCCCCGCCAACG‑ATGTGTTGACGGGGCTGTTATTATTTTGGCAATAATACTCCGGTATAAGTATTTACCGGATGAGAAAGATATTGTTTAACGGCAGTGTTAACATTCTCTACCGTCATTTGttt                                           <  1:1037455/149‑1 (MQ=255)                                                                                                      tAAAAATAGCGAATATTGCTAAAATCCCCGCCAACG‑ATGTGTTGACGGGGCTGTTATTATTTTGGCAATAATACTCCGGTATAAGTATTTACCGGATGAGAAAGATATTGTTTAACGGCAGTGTTAACATTCTCTACCGTCATTTGttt                                           >  1:756437/1‑149 (MQ=255)                                                                                                         aaaTAGCGAATATTGCTAAAATCCCCGCCAACG‑ATGTGTTGACGGGGCTGTTATTATTTTGGCAATAATACTCCGGTATAAGTATTTACCGGATGAGAAAGATATTGTTTAACGGCAGTGTTAACATTCTCTACCGTCATTTGTTTcaa                                        <  2:382017/149‑1 (MQ=255)                                                                                                              gCGAATATTGCTAAAATCCCCGCCAACG‑ATGTGTTGACGGGGCTGTTATGATGTTGGCAATAATACTCCGGTATAAGTATTTACCGGATGAGAAAGATATTGTTTAACGGCAGTGTTAACATTCTCTACCGTCATTTGTTTCAACAAtt                                   <  1:1067369/149‑1 (MQ=255)                                                                                                               cGAATATTGCTAAAATCCCCGCCAACG‑ATGTGTTGACGGGGCTGTTATTATTTTGGCAATAATACTCCGGTATAAGTATTTACCGGATGAGAAAGATATTGTTTAACGGCAGTGTTAACATTCTCTACCGTCATTTGTTTCAACAATTg                                  <  1:1249023/149‑1 (MQ=255)                                                                                                                gAATATTGCTAAAATCCCCGCCAACG‑ATGTGTTGACGGGGCTGTTATTATTTTGGCAATAATACTCCGGTATAAGTATTTACCGGATGAGAAAGATATTGTTTAACGGCAGTGTTAACATTCTCTACCGTCATTTGTTTCAACAATTGc                                 >  2:623659/1‑149 (MQ=255)                                                                                                                gAATATTGCTAAAATCCCCGCCAACG‑ATGTGTTGACGGGGCTGTTATTATTTTGGCAATAATACTCCGGTATAAGTATTTACCGGATGAGAAAGATATTGTTTAACGGCAGTGTTAACATTCTCTACCGTCATTTGTTTCAACAATTGc                                 <  1:568771/149‑1 (MQ=255)                                                                                                                gAATATTGCTAAAATCCCCGCCAACG‑ATGTGTTGACGGGGCTGTTATTATTTTGGCAATAATACTCCGGTATAAGTATTTACCGGATGAGAAAGATATTGTTTAACGGCAGTGTTAACATTCTCTACCGTCATTTGTTTCAACAATTGc                                 >  2:190404/1‑149 (MQ=255)                                                                                                                                 ccGCCAACG‑ATGTGTTGAcggggattttagcaatattcgctatttttatgtaataattttataaatgcgttcaaaataataatcaagtactaatagtgatattttaaggtttgatttttacgtgataattcaggagacacagaatgcgc                <  1:1204619‑M2/149‑132 (MQ=255)                                                                                                                                 ccGCCAACG‑ATGTGTTGACGGGGCTGTTATTATTTTGGCAATAATACTCCGGTATAAGTATTTACCGGATGAGAAAGATATTGTTTAACGGCAGTGTTAACATTCTCTACCGTCATTTGTTTCAACAATTGCTCCTGCTCAGTCCAtgc                <  1:1099776/149‑1 (MQ=255)                                                                                                                                           gtttgtTGACGGGGCTGTTATTATTTGTGCAATAATACTCCGGTATAAGTATTTACCGGATGAGAAAGATATTGTTTAACGGCAGTGTTAACATTCTCTACCGTCATTTGTTTCAACAATTGCTCCTGCTCAGTCCATGCTGCAGGATc       <  2:1289722/146‑1 (MQ=255)                                                                                                                                                ttGACGGGGCTGTTATTATTTTGGCAATAATACTCCGGTATAAGTATTTACCGGATGAGAAAGATATTGTTTAACGGCAGTGTTAACATTCTCTACCGTCATTTGTTTCAACAATTGCTCCTGCTCGGTCCATGCTGCCGGATCGTCat  >  1:355287/1‑149 (MQ=255)                                                                                                                                                ttGACAGGGCTGTTATTCTTTTGACAATAATACTCCGGTATAAGTATTTACCGGATGAGAAAGATATTGTTTAACGGCAGTGTTAACATTCTCTACCGTCATTTGTTTCAACAATTGCTCCTGCTCAGTCCATGCTGCAGGATCGTCat  <  2:647639/149‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                  GCGCATTCTGTGTCTCCTGAATTATCACGTAAAAATCAGACCTTAAAATATCACTATTAGTACTTGATTATTATTTTGAACGCATTTATAAAATTATTACATAAAAATAGCGAATATTGCTAAAATCCCCGCCAACG‑ATGTGTTGACGGGGCTGTTATTATTTTGGCAATAATACTCCGGTATAAGTATTTACCGGATGAGAAAGATATTGTTTAACGGCAGTGTTAACATTCTCTACCGTCATTTGTTTCAACAATTGCTCCTGCTCAGTCCATGCTGCAGGATCGTCAT  >  NC_000913/1572250‑1572540

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.