Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,793,898	Δ1 bp	coding (212/552 nt)	btuE ←	glutathione peroxidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,793,897	0	T	.	97.3%	330.2 / 0.6	76	coding (213/552 nt)	btuE	glutathione peroxidase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base . (40/33);  minor base C (1/1);  total (42/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTGAGGCGACATTGACAATCAACAGCACATTACCGGCG  >  NC_000913/1793753‑1794041                                                                                                                                                 |                                                                                                                                                 gACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCtgttc                                                                                                                                              <  2:1009625/149‑5 (MQ=255)  aCTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCGATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTcc                                                                                                                                            <  1:871021/149‑1 (MQ=255)       ggTCGGCGCTGCGGCAATCATTTTTTGATACAGCTGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGaaa                                                                                                                                       <  1:564786/149‑1 (MQ=255)         tCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGACACt                                                                                                                                     >  2:1148471/1‑149 (MQ=255)           ggCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACttg                                                                                                                                   >  1:1229728/1‑147 (MQ=255)              gcTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTg                                                                                                                                >  1:1235583/1‑149 (MQ=255)                   ggCAGTCAATTTTTTATACAGCGGATTGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACgg                                                                                                                           <  2:108008/149‑1 (MQ=255)                   ggCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACACTAAGTTTTAATCTCCGCTTCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACgg                                                                                                                           <  1:1205885/149‑1 (MQ=255)                   ggCAATCAATTTTTGAGACAGCGGATGGCGTCCTTCGCCATTACCTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTGTAATCTCTTCAGCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACgg                                                                                                                           <  2:1337315/149‑1 (MQ=255)                    gCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACggg                                                                                                                          >  1:222080/1‑149 (MQ=255)                       atcaatTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAAt                                                                                                                       >  1:1246784/1‑149 (MQ=255)                        tcaatTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCCGAAACTGGTTGCACGGGAATc                                                                                                                      >  2:1100773/1‑149 (MQ=255)                        tcaatTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATc                                                                                                                      >  1:334137/1‑149 (MQ=255)                        tcaatTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATc                                                                                                                      >  1:379399/1‑149 (MQ=255)                        tcaatTTTGGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGG‑TCCTGTTCCGGAAACTGGTTGCACGGGAATc                                                                                                                      >  2:14484/1‑149 (MQ=255)                          aatTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATccc                                                                                                                    <  2:788060/149‑1 (MQ=255)                            tttttGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAg                                                                                                                  <  2:1235583/149‑1 (MQ=255)                            atgttGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAAGCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAg                                                                                                                  <  2:729504/146‑1 (MQ=255)                                 gATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCAGGTTC‑TGTTCCAGTAACTGGTTGCACGGGTATCCCAGCACCa                                                                                                             >  2:374075/1‑149 (MQ=255)                                  aTACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGCTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCAt                                                                                                            >  2:419050/1‑149 (MQ=255)                                   tACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCGGAAACGGGTTGCACGGGAATCCAAGCACCaaa                                                                                                           >  2:1044042/1‑147 (MQ=255)                                           aTGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACAAACCCTGTGGTGGTACAGTGAGTTTTAATCTCTTCAGAGCTGCCCGATTC‑TGTTCCAGAAACTGGATGCACGGGAATCCCGGCCCCATAAAACCtcg                                                                                                   >  2:120667/1‑149 (MQ=255)                                             ggCGTGCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCtcgat                                                                                                 <  2:172975/149‑1 (MQ=255)                                             ggCGTCCTTCGCCATCACCTTCAATCTTACTGAACATCGGGAACGCCCCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCtcgat                                                                                                 <  2:103670/149‑1 (MQ=255)                                              gCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCtcgatc                                                                                                <  1:517958/149‑1 (MQ=255)                                                   cTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGAccc                                                                                           >  2:263231/1‑149 (MQ=255)                                                           ttAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTTGTTGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTc                                                                                   <  1:33779/149‑1 (MQ=255)                                                           ttAACTTCAATCTTACTGAACATCGGAAACGTCACCCACCATGTGGGGGTACAGTATGATTTAATATCTTCGTCGCTGCCCGGTTC‑TGTTCCAAAAACTGGTTGCACCGACATCCCGGCCCCATAAAACCCCGATCCCCCCGGGCTTTc                                                                                   >  2:521210/1‑149 (MQ=255)                                                            tAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCt                                                                                  <  2:1208995/149‑1 (MQ=255)                                                            tAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCt                                                                                  <  1:77679/149‑1 (MQ=255)                                                            tAACCTCAATCTTACTTAACATCGGGACCGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCt                                                                                  <  2:467738/149‑1 (MQ=255)                                                              aCTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGa                                                                                <  2:1246784/149‑1 (MQ=255)                                                              aCTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGa                                                                                <  1:1326949/149‑1 (MQ=255)                                                                ttCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATCAAACCTCGATCGACCCAGGCTTTCTGAat                                                                              >  2:888110/1‑149 (MQ=255)                                                                ttCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAat                                                                              >  1:472417/1‑149 (MQ=255)                                                                     tCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCtgttc                                                                                                                                              <  1:833777/81‑5 (MQ=255)                                                                     tCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCtgttc                                                                                                                                              >  2:833777/1‑77 (MQ=255)                                                                      cTTCCTGAACCTCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATtctc                                                                        <  1:417289/149‑1 (MQ=255)                                                                         aCTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGCGTCCCAGCACCATAAAACCCCGCCCGACTCAGACTTTCTGAATATTCTCCaa                                                                     >  2:558160/1‑149 (MQ=255)                                                                            gAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTg                                                                  >  2:758310/1‑149 (MQ=255)                                                                            gAACAGCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGGCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGCCCCAGGCTTTCTGAATATTCTCCAACTg                                                                  <  2:378040/149‑1 (MQ=255)                                                                             aaCATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGc                                                                 >  2:173905/1‑149 (MQ=255)                                                                               cATCTGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTc                                                               <  2:1073081/149‑1 (MQ=255)                                                                                   gggAACGTCACCCCCCATGTGGAGGTACAGTAAGTTCTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCatat                                                           >  2:1242698/1‑149 (MQ=255)                                                                                   gggAACGTCACCCCCAATGTGGGGGTACAGTAAGTTTCAATCTCTCCACCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCATCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCatat                                                           <  2:260186/149‑1 (MQ=255)                                                                                        cGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTgcgg                                                      >  2:147219/1‑149 (MQ=255)                                                                                             ccccccATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGGTa                                                 >  1:869201/1‑149 (MQ=255)                                                                                              ccccccTGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGTGGCGTTaa                                                <  1:449808/149‑1 (MQ=255)                                                                                              cccccATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTaa                                                >  1:368529/1‑149 (MQ=255)                                                                                              cccccATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTCCTCATATTGCGGCGttct                                                >  2:1200385/1‑147 (MQ=255)                                                                                              cccccATGTGGTGGTACAGTAAGTTTTAATCTCGTCATCGCCGCACGG‑TCCTCCTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTaa                                                <  1:521438/149‑1 (MQ=255)                                                                                               ccccATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAg                                               >  2:1190415/1‑149 (MQ=255)                                                                                                cccATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGc                                              >  1:1344895/1‑149 (MQ=255)                                                                                                cccATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGc                                              <  1:1022795/149‑1 (MQ=255)                                                                                                  cATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATAGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCca                                            >  2:750800/1‑149 (MQ=255)                                                                                                      tggtggTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTCCGCCGTTAAGCCAGACt                                        >  2:526422/1‑149 (MQ=255)                                                                                                         tggtACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTg                                     <  2:1233273/149‑1 (MQ=255)                                                                                                           gtACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTGAg                                   >  1:441664/1‑149 (MQ=255)                                                                                                           gtACAGTAAGTTTTAATCTCTTCACCGCTTCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGCATCCCAGCACCATAAAACCTCGATCTACCCAGGCTGTCTGAATATTCTCCAACTGATCATATTGCGGCGTTAAGCCACACTTTGAg                                   <  2:7315/149‑1 (MQ=255)                                                                                                                gTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCCTATTGCGGCGTTATGGTGCACTTTGAGGCGAc                              >  1:981095/1‑149 (MQ=255)                                                                                                                gTAAGTTTTAATCTCTTCATCGCTGCCCGG‑ACATGTAGCTTAAACTGGTTTCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAATCCACGCTTTGGGGCGAc                              >  2:295504/1‑149 (MQ=255)                                                                                                                 tAAGTTTTAATCTCTTCATCGCTGCGCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTGAGGCGAcc                             >  1:809749/1‑148 (MQ=255)                                                                                                                 tAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTGAGGCGACa                             >  2:1217835/1‑149 (MQ=255)                                                                                                                 tAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTGAGGCGACa                             >  2:700715/1‑149 (MQ=255)                                                                                                                     ttttAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCATGGTAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTGAGGCGACATTGa                         <  1:1049565/149‑1 (MQ=255)                                                                                                                      tttAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTGAGGCGACATTGAc                        <  2:379399/149‑1 (MQ=255)                                                                                                                       ttAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAACCCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTGAGGCGACATTGACa                       <  1:441487/149‑1 (MQ=255)                                                                                                                        tAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTGAGGCGACATTGACaa                      <  1:1242698/149‑1 (MQ=255)                                                                                                                         aaTCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGCCCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTGAGGCGACATTGACAAt                     <  2:222080/149‑1 (MQ=255)                                                                                                                          aTCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGCATATTCTCCAACTGCTCATATTGCCGCGTTAAGCCACACTTTGATGCGACATTGACAATc                    <  2:664284/149‑1 (MQ=255)                                                                                                                           tctcTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTGAGGCGACATTGACAATCa                   >  1:1145052/1‑149 (MQ=255)                                                                                                                           tctcTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGCTGCACGGGCATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTCAGCCACCCTTTGGGGCGACACTgcgaaaca                   >  2:1219055/1‑142 (MQ=255)                                                                                                                            ctctTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTGAGGCGACATTGACAATCaa                  <  2:193532/149‑1 (MQ=255)                                                                                                                            ctctTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTGAGGCGACATTGACAATCaa                  >  1:1083706/1‑149 (MQ=255)                                                                                                                             tctTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTGAGGCGGCCTTGCCAATCAAc                 >  2:825621/1‑149 (MQ=255)                                                                                                                                  aTCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCCGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTGAGGCGCCATTGACAATCAACAGcac            >  1:636221/1‑149 (MQ=255)                                                                                                                                      cTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTgcg                                                       <  1:223020/102‑1 (MQ=255)                                                                                                                                      cTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTgcg                                                       >  2:223020/1‑102 (MQ=255)                                                                                                                                            ggTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTGAGGCGACATTGACAATCAACAGCACATAACCGGCg  >  2:1208232/1‑149 (MQ=255)                                                                                                                                                 |                                                                                                                                                 GACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTGAGGCGACATTGACAATCAACAGCACATTACCGGCG  >  NC_000913/1793753‑1794041

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑