| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 4,131,611 | A→C | 66.9% | T593P (ACC→CCC) | metL → | Bifunctional aspartokinase/homoserine dehydrogenase 2 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,131,611 | 0 | A | C | 66.9% | 123.6 / 56.4 | 74 | T593P (ACC→CCC) | metL | Bifunctional aspartokinase/homoserine dehydrogenase 2 |
| Reads supporting (aligned to +/- strand): ref base A (12/13); new base C (20/29); total (32/42) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.24e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTGAGCTATGACGGGCTGGACGCCAGCCGCGCGTTAGCCTTCTTCAACGATGAAGCGGTTGAGCAGGATGAAGAGTCGTTGTTCCTGTGGATGCGCGCCCATCCGTATGATGATTTAGTGGTGCTGGACGTTACCGCCAGCCAGCAGCTTGCTGATCAGTATCTTGATTTCGCCAGCCACGGTTTCCACGTTATCAGCGCCAACAAACTGGCGGGAGCCAGCGACAGCAATAAATATCGCCAGATCCACGACGCCTTCGAAAAAACCGGGCGTCACTGGCTGTACAATGCCACCGTCGGTGCGGGCTT‑GCCGATCAACCACACCGTGCGCGATCTGATCGACAGCGGCGATACTATTTTGTCGATCAGCGGGATCTTCTCCGGCACGCTCTCCTGGCTGTTCCTGCAATTCGACGGTAGCGTGCCGTTTACCGAGCTGGTGGATCAGGCGTGGCAGCAGGGCTTAACCGAACCTGACCCGCGTGACGATCTCTCTGGCAAAGACGTGATGCGCAAGCTGGTGATTCTGGCGCGTGAAGC > NC_000913/4131347‑4131885 | tagAGCTATGACGGGCTGGACGCCAGCCGCGCGTTAGCCTTCTTCAACGATGAAGCGGTTGAGCAGGATGAAGAGTCGTTGTTCCTGTGGATGCGCGCCCATCCGTATGATGATTTAGTGGTGCTGGACGTTACCGCCAGCCAGCAGCTTGCTGATCAGTATCTTGATTTCGCCAGCCACGGTTTCCACGTTATCAGCGCCAACAAACTGGCGGGAGCCAGCGACAGCAATAAATATCGCCAGATCCACGACGCCTTCGAAAAAACCGGGCGTc > 2:499115/3‑274 (MQ=255) tGAGCTATGACGGGCTGGACGCCAGCCGCGCGTTAGCCTTCTTCAACGATGAAGCGGTTGAGCAGGATGAAGAGTCGTTGTTCCTGTGGATGCGCGCCCATCCGTATGATGATTTAGTGGTGCTGGACGTTACCGCCAGCCAGCAGCTTGCTGATCAGTATCTTGATTTCGCCAGCCACGGTTTCCACGTTATCAGCGCCAACAAACTGGCGGGAGCCCGCGACAGCAATAACTATCGCCAGATCCACGACCCCTTCGAAAAACACGGGCGTCACTgg > 1:142472/1‑278 (MQ=255) tGAGCTATGACGGGCTGGACGCCAGCCGCGCGTTAGCCTTCTTCAAAGATGAAGCGGTTGAGCAGGATGAAGAGTCGTTGTTCCTGTGGATGCGCGCCCATCCGTATGATGATTTAGTGGTGCTGGACGTTACCGCCAGCCAGCAGCTTGCTGATCAGTATCTTGATTTCGCCAGCCACGGTTTCCACGTTATCAGCGCCAACAAACTGGCGGGAGCCAGCGACAGCAATAAATATCGCCAGATCCACGACGCCTTCGAAAAACCCGGGCGTCACTgg < 1:53425/278‑1 (MQ=255) gAGCTATGACGGGCTGGACGCCAGCCGCGCGTTAGCCTTCTTCAACGATGAAGCGGTTGAGCAGGATGAAGAGTCGTTGTTCCTGTGGATGCGCGCCCATCCGTATGATGATTTAGTGGTGCTGGACGTTACCGCCAGCCAGCAGCTTGCTGATCAGTATCTTGATTTCGCCAGCCACGGTTTCCACGTTATCAGCGCCAACAAACTGGCGGGAGCCAGCGACAGCAATAAATATCGCCAGATCCACGACGCCTTCGAAAAACCCGGGCGTc < 2:104970/272‑1 (MQ=255) gAGCTATGACGGGCTGGACGCCAGCCGCGCGTTAGCCTTCTTCAACGATGAAGCGGTTGAGCAGGATGAAGAGTCGTTGTTCCTGTGGATGCGCGCCCATCCGTATGATGATTTAGTGCTGCTGGACGTTACCGCCAGCCAGCAGCTTGCTGATCAGTATCTTGATTTCGCCAGCCACGGTTTCCACGTTATCAGCGCCAACAAACTGGCGGGAGCCAGCGACAGCAATAAATATCGCCAGATCCACGACGCCTTCGAAAAAACCGGGCGTCa < 2:44272/273‑1 (MQ=255) cGGGCTGCACGCCAGCCGCGCTTTAGCCTTCTTCAACGATGATGCGGTTGAGCAGGATGAATAGTCGTTGTTCCTGTGGATGCGCGCCACTCCGTATGATGATTTAGCGGTGCTGGACGTTACCGCCAGCCAGCAGCTTGCTGATCAGTATCTTGATTTCGCCAGCCACGGTTTCCACGTTATCAGCGTCAACAAACTGGCGGGAGCCAGCGACAGCAATAAATATCGCCAGATCCACGACGCCTTCGAAAAAACCGGGCGTCACTGGCTGTa < 2:280098/273‑1 (MQ=255) ggCTGGACGCCAGCCGCGCGTTAGCCTTCTTCAACGATGAAGCGGTTGAGCAGGATGAAGAGTCGTTGTTCCTGTGGATGCGCGCCCATCCGTATGATGATTTAGTGGTGCTGGACGTTACCGCCAGCCAGCAGCTTGCTGATCAGTATCTTGATTTCGCCAGCCACGGTTTCCACGTTATCAGCGCCAACAAACTGGCGGGAGCCAGCGACAGCAATAAATATCGCCAGATCCACGACGCCTTCGAAAAAACCGGGCGTCACTGGCTGTACAAt > 2:239907/1‑275 (MQ=255) cTGGACGCCAGCCGCGCGTTAGCCTTCTTCAACGATGAAGCGGTTGAGCAGGATGAAGAGTCGTTGTTCCTGTGGATGCGCGCCCATCCGTATGATGATTTAGTGGTGCTGGACGTTACCGCCAGCCAGCAGCTTGCTGATCAGTATCTTGATTTCGCCAGCCACGGTTTCCACGTTATCAGCGCCAACAAACTGGCGGGAGCCAGCGACAGCAATACATACCGCCAGATCCACGACGCCTTCGATAAACCCGGGCTCCACTGGCTGTACactcc > 2:449841/1‑271 (MQ=255) cTGGACGCCAGCCGCGCGTTAGCCTTCTTCAACGATGAAGCGGTTGAGCAGGATGAAGAGTCGTTGTTCCTGTGGATGCGCGCCCATCCGTATGATGATTTAGTGGTGCTGGACGTTACCGCCAGCCAGCAGCTTGCTGATCAGTATCTTGATTTCGCCAGCCACGGTTTCCACGTTATCAGCGCCAACAAACTGGCGGGAGCCAGCGACAGCAATAAATATCGCCAGATCCACGACGCCTTCGAAAAACCCGGGCGTCACTGGCTGTACAATGCCAcc > 1:61866/1‑279 (MQ=255) gACGCCAGCCGCGCGTTAGCCTTCTTCAACGATGAAGCGGTTGAGCAGGATGAAGAGTCGTTGTTCCTGTGGATGCGCGCCCATCCGTATGATGATTTAGTGGTGCTGGACGTTACCGCCAGCCAGCAGCTTGCTGATCAGTATCTTGATTTCCCCAGCCACGGTTTCCACGTTATCAGCGCCAACAAACTGGCGGGAGCCAGCGACAGCAATAAATATCGCCAGATCCACGACGCCTTCGAAAAACCCGGGCGTCACTGGCTGTACAATGCCAc > 2:342263/1‑275 (MQ=255) gggttgtgcttcctTAACGATGGAGCGGGTGNNGAGGATGTGGTGTCGTTGGTCCTGTGGATGNGCTCCCATCCGTATGATGATTTAGTGGTGCTGGACGTTACCGCCAGCCAGCAGCTTGCTGATCAGTATCTTGATNTCGCCAGCCACGGTTTCCACGTTATCAGCGCCAACAAACTGGCGGGAGCCAGCGACAGCAATAAATATCGCCAGATCCACGACGCCTTCGAACAACCCGGGCGTCACTGGCTGTACAATGCCACCGTCGGTGCGGGCt < 3:80080/269‑1 (MQ=255) tgaGCTTTTTTCAACGAGGAGGGGGTTTAGCGNGNGTAGGATTCGTTGTTCCTGTGGAGGCGCTCCCCTCCGTATGATGTNTTAGTGGTGCTGGACGTTGCCGCCTGCCAGCAGCTTGCTGATCAGTATCTTGATTTCGCCAGCCACGGTNTCCACGTTATCAGCGCCAACAAACTGGCGGGAGCAAGCGAAAGCAATAAATATCGCCAGATCCACGACGCCTTCGAAAAACCCGGGCGTCACGGGCTGTACAATGCCACCGTCGGTGCGGGCTT‑GCCg < 3:48987/277‑1 (MQ=255) gCCTGCTTGAAGGATGAATGGGTTGAGAGGGATGAAGGGTCTTTGTTCCGTTGGATGCGCGCCCATCCGTATGATGATTTAGTGGTGCTGGACGTTACCGCCAGCCAGCAGCTTGCTGATCAGTATCTTGATTTCGCCAGCCACGGTTTCCACGTTATCAGCGCCAACAAACTGGCGGGAGCCAGCGACAGCAATAAATATCGCCAGATCCACGACGCCTTCGAAAAAACCGGGCGTCACTGGCTGTACAATGCCACCGTCGGTGCGGGCTT‑Gc < 4:239907/274‑1 (MQ=255) ccTTCTTCAACGAGGAAGCGGTTGGGCAGGATGAAGGGTCGTTTTACCTGTGGATCCGCGCCCATCCGTATGATGATTTAGTGGTGCTGGACGTTACCGCCAGCCAGCAGCTTGCTGATCAGTATCTTGATTTCGCCAGCCACGGTTTCCACGTTATCAGCGCCAACAAACTGGCGGGAGCCAGCGACAGCAATAAATATCGCCAGATCCACGACGCCTTCGAAAAACCCGGGCGTCACTGGCTGTACAATGCCACCGTCGGTGCGGGCTT‑GCCg < 2:20708/275‑1 (MQ=255) ccTGCTTCAACGATGAAGCGGTGGGGCAGGATGAAGGGTCGTTGTTCCTGTGGATGCGCGCCCATCCGTATGATGATTTAGTGGTGCTGGACGTTACCGCCAGCCAGCAGCTTGCTGATCAGTATCTTGATTTCCCCAGCCACGGTTTCCACGTTATCAGCGCCAACAAACTGGCGGGAGCCAGCGACAGCAATAAATATCGCCAGATCCACGACGCCTTCGAAAAACCCGGGCGTCACTGGCTGTACAATGCCACCGTCGGTGCGGGCTT‑GCCg < 4:342263/275‑1 (MQ=255) tcttcAACGATGAAGCGGTTGAGCAGGATGAAGAGTCGTGGTTCCTGTGGATGCGCGCCCAGCCGTATGATGATTTAGTGGTGCTGGACGTTACCGCCAGCCAGCAGCTTGCTGATCAGTATCTTGATTTCGCCAGCCACGGTTTCCACGTTATCAGCGCCAACAAACTGGCGGGAGCCAGCGACAGCAATAAATATCGCCAGATCCACGACGCCTTCGAAAAACCCGGGCGTCACTGGCTGTACAATGCCACCGTCGGTGCGGGCTT‑GCCGAt < 2:182931/274‑1 (MQ=255) cttcAACGATGAAGCGGTTGAGCAGGATGAAGAGTCGTTGTTCCTGTGGATGCGCGCCCATCCGTATGATGATTTAGTGGTGCTGGACGTTACCGCCAGCCAGCAGCTTGCTGATCAGTATCTTGATTTCGCCAGCCACGGTTTCCACGTTATCAGCGCCAACAAACTGGCGGGAGCCAGCGACAGCAATAAATATCGCCAGATCCACGACGCCTTCGAAAAACCCGGGCGTCACTGGCTGTACAATGCCACCGTCGGTGAGGGCTT‑GCCGATCa > 2:271580/1‑275 (MQ=255) aaGCGGTTGAGCAGGATGAAGAGTCGTTGTTCCTGTGGATGCGCGCACATCCGTATGATGATTTAGTGGTGCTGGACGTTACCGCCAGCCAGCAGCTTGCTGATCAGTATCTTGATTTCGCCAGCCACGGTTTCCACGTTATCAGCGCCAACAAACTGGCGGGAGCCAGCGACAGCAATAAATATCCCCAGATACACGACCCACTAGAAAAACCCGGGCGGAACTTGCTGGACAATGCCACCGTCGGCCCGCGCTT‑ACCGATCAAcccacccat > 4:564009/1‑267 (MQ=255) ggTTGGGCAGGATGAAGGGTCTTTGTTCCTGTGGTTGCGCGCCCGTCCGTATGATGATTTAGTGGTGCTGGACGTTACCGCCAGCCAGCAGCTTGCTGATCAGTATCTTGATTTCGCCAGCCACGGTTTCCACGTTATCAGCGCCAACAAACTGGCGGGAGCCAGCGACAGCAATAAATATCGCCAGATCCACGACGCCTTCGAAAAACCCGGGCGTCACTGGCTGTACAATGCCACCGTCGGTGCGGGCTT‑GCCGATCAACCACACCGTGcgcg < 2:562407/275‑1 (MQ=255) ggATGAAGGTTCGTTGTTCCTGTGGATGCGCGCCCATCCTTATGATGATTTAGTGTGTCTGGACTTTACCGCCAGCCATCAGCTTGCTGATCATTATCTTGATTTCGCCAGCCACGGTTTCCACGTTATCAGCGCCAACAAACTGGCGGGAGCCAGCGACAGCAATAAATATCGCCAGATCCACGACGCCTTCGAAAAACCCGGGCGTCACTGGCTGTACAATGCCACCGTCGGTGCGGGCTT‑GCCGATCAACCACACCGTGCGCGATCTGATCGAc < 1:71534/277‑1 (MQ=255) tGAAGAGTCGTTGTTCCTGTGGATGCGCGCCCATCCGTATGATGATTTAGTGGTGCTGGACGTTACCGCCCGCCAGCAGCTTGCTGATCAGTATCTTGATTTCGCCAGCCACGGTTTCCACGTTATCAGCGCCAACAAACTGGCGGGCGACAGCGACAGCAATAAATATCGCCCGATCCACGACGCCTTCGAAAAACCCGGGAGTCACTGGCTGTACAATGCCACCGTCGGGGCGGGCTTCGCCGA‑CCCCCCCACCCTGcgcggacagctagaca > 4:126680/1‑263 (MQ=255) gagTCGTTGTTCCTGTGGATGCGCGCCCATCCGTATGATGATTTAGTGGTGCTGGACGTTACCGCCAGCCAGCAGCTTGCTGATCAGTATCTTGATTTCGCCAGCCACGGTTTCCACGTTATCAGCGCCAACAAACTGGCGGGAGCCAGCGACAGCAATAAATATCGCCAGATCCACGACGCCTTCGAAAAAACCGGGCGTCACTGGCTGTACAATGCCACCGTCGGTGCGGGCTT‑GCCGATCAACCACACCGGGCGCGATCTGATCGACAgcgg > 2:438152/1‑275 (MQ=255) cGTTGTTCCTGTGGATGCGCGCCCATCCGTATGATGATTTAGTGGTGCTGGACGTTACCGCCAGCCAGCAGCTTGCTGATCAGTATCTTGATTTCGCCAGCCACGGTTTCCACGTTATCAGCGCCAACAAACTGGCGGGAGCCAGCGACAGCAATAAATATCGCCAGATCCACGACGCCTTCGAAAAACCCGGGCGTCACTGGCTg < 1:21587/206‑1 (MQ=255) tttcTGTGGATGCGCGCCCATCCGTATGATGATTTAGTGGTGCTGGACGTTACCGCCAGCAAGCAGCTTGCTGATCAGTATCTTGATTTCGCCAGCCACGGTTTCCACGTTATCAGCGCCAACAAACTGGCGGGAGCCAGCGACAGCAATAAATATCGCCAGATCCACGACGCCTTCGAAAAACCCGGGCGTCACTGGCTGTACAATGCCACCGTCGGTGCGGGCTT‑GCCGATCAACCACACCGTGCGCGATCTGATCGACAGCGGCGATACTAATTTg < 1:176715/276‑1 (MQ=255) gtgGATGCGCGCCCATCCGTATGATGATTTAGTGGTGCTGGACGTTACCGCCAGCCAGCAGCTTGCTGATCAGTATCTTGATTTCGCCAGCCACGGTTTCCACGTTATCAGCGCCAACAAACTGGCGGGAGCCAGCGACAGCAATAAATATCGCCAGATCCACGACGCCTTCGAAAAAACCGGGCGTCACTTGCTGTACAATGCCACCGTCGGTGCGGGCTT‑GCCGATCAACCACACCGTGCGCGATCTGATCGACAGCGGCGATACTAtttt > 2:3326/1‑273 (MQ=255) gATTCGCGCCAATCCGTTTGATGATTTAGTGGTGCTGGACGTTACCGCCGGCCAGCAGCTTGCTGATCAGGATCTTGAGTTCGCCAGCAACGGTTTCCACGTTATCAGCGCCAACAAACTGGCGGGAGCCAGCGACAGCAATAAATATCGCCAGATCCACGACGCCTTCGAAAAAACCGGGCGTCACTGGCTGTACAATGCCACCGTCGGTGCGGGCTT‑GCCGATCAACCACACCGTGCGCGATCTGATCGACAGCGGCGATACTATTTTGTCg < 4:438152/274‑1 (MQ=255) gcCCATCCGTATGATGATTTAGTGGTGCTGGACGTTACCGCCAGCCAGCAGCTTGCTGATCAGTATCTTGATTTCGCCAGCCACGGTTTCCACGTTATCAGCGCCAACAAACTGGCGGGAGCCAGCGACAGCAATAAATATCGCCAGATCCACGACGCCTTCGAAAAACCCGGGCGTCACTGGCTGTACAATGCCACCGTCGGTGCGGGCTT‑GCCGATCAACCACACCGTGCGCGATCTGATCGACAGCGGCGATACTATTTTGTCGATCAGCg < 2:204947/274‑1 (MQ=255) cGTATGATGTGTTAGTGGTGCCGTACTTTACCGCCAGCCAGCAGCTTGCTGATCAGTATCTTGATTTCGCCAGCCACGGTTTCCACGTTATCAGCGCCAACAAACTGGCGGGAGCCAGCGACAGCAATAAATATCGCCAGATCCACGACGCCTTCGAAAAACCCGGGCGTCACTGGCTGTACAATGCCACCGTCGGTGCGGGCTT‑GCCGATCAACCACACCGTGCGCGATCTGATCGACAGCGGCGATACTATTTTGTCGATCAGCGGGAtctt < 2:126680/274‑1 (MQ=255) atTTGGTGGTGGTTGCCGTTCCCTCCAGCCACGTGCTTGCTGATCAGTCTCTTGATTTCGCCGGCCACGGTTTCCACGTTATCAGCGCCAACAAACTGGCGTGAGCCACCGACAGCAATAAATATCGCCAGATCCACGACGCCTTCGAAAAAACCGGGCGTCACTGGCTGTACAATGCCACCGTCGGTGCGGGCTT‑GCCGATCAACCACACCGTGCGCGATCTGATCGACAGCGGCGATACTATTTTGTCGATCAGCGGGATCTTCTCCGGCACg < 4:471669/275‑1 (MQ=255) atTTAGTGGTGCTGGACGTTACCGCCAGCCAGCAGCTTGCTGATCAGTATCTTGATTTCGCCAGCCACGGTTTCCACGTTATCAGCGCCAACAAACTGGCGGGAGCCAGCGACAGCAATAAATATCGCCAGATCCACGACGCCTTCGAAAAAACCGGGCGTCACTGGCTGTACAATGCCACCGTCGGTGCGGGCTT‑GCCGATCAACCACACCGTGCGCGATCTGATCGACAGCGGCGATACTATTTTGTCGATCAGCGGGATCTTCTCCGGCACg < 2:421696/275‑1 (MQ=255) ttAGTGGTGCTGGACGTTACCGCCAGCCAGCAGCTTGCTGATCAGTATCTTGATTTCGCCAGCCACGGTTTCCACGTTATCAGCGCCAACAAACTGGCGGGAGCCAGCGACAGCAATAAATATCGCCAGATCCACGACGCCTTCGAAAAAACCGGGCGTCACTGGCTGTACAATGCCACCGTCGGTGCGGGCTT‑GCCGATCAACCACACCGTGCGCGATCTGATCGACAGCGGCGATACTATTTTGt > 2:73568/1‑247 (MQ=255) tggtgCTGGACGTTACCGCCAGCCAGCAGCTTGCTGATCAGTATCTTGATTTCGCCAGCCACGGTTTCCACGTTATCAGCGCCAACAAACTGGCGGGAGCCAGCGACAGCAATAAATATCGCCAGATCCACGACGCCTTCGAAAAAACCGGGCGTCACTGGCTGTACAATGCCACCGTCGGTGCGGGCTT‑GCCGATCAACCACACCGTGCGCGATCTGATCGACAGCGGCGATACTATTTTGTCGAt < 4:73568/247‑1 (MQ=255) tggtgCTGGACGTTACCGCCAGCCAGCAGCTTGCTGATCAGTATCTTGATTTCGCCAGCCACGGTTTCCACGTTATCAGCGCCAACAAACTGGCGGGAGCCAGCGACAGCAATAAATATCGCCAGATCCACGACGCCTTCGAAAAAACCGGGCGTCACTGGCTGTACAATGCCACCGTCGGTGCGGGCTT‑GCCGATCAACCACACCGTGCGCGATCTGATCGACAGCGGCGATACTATTTTGTCGATCAGCGGGATCTTCTCCGGCACGCTCTcc > 2:44819/1‑275 (MQ=255) gCTGGACGTTACCGCCAGCCAGCAGCTTGCTGATCAGTATCTTGATTTCGCCAGCCACGGTTTCCACGTTATCAGCGCCAACAAACTGGCGGGAGCCAGCGACAGCAATAAATATCGCCAGATCCACGACGCCTTCGAAAAACCCGGGCGTCACTGGCTGTACAATGCCACCGTCGGTGCGGGCTT‑GCCGATCAACCACACCGTGCGCGATCTGATCGACAGCGGCGATACTCTTTTGTCGATCAGCGGGATCTTCTCCGGCACGCCCTCCTgg > 2:555181/1‑274 (MQ=255) gCTGGACGTTACCGCCAGCCAGCAGCTTGCTGATCAGTATCTTGATTTCGCCAGCCACGGTTTCCACGTTATCAGCGCCAACAAACTGGCGGGAGCCAGCGACAGCAATAAATATCGCCAGATCCACGACGCCTTCGAAAAACCCGGGCGTCACTGGCTGTACAATGCCACCGTCGGTGCGGGCTT‑GCCGATCAACCACACCGTGCGCGATCTGATCGACAGCGGCGATACTATTTTGTCGATCAGCGGGATCTTCTCCGGCACGCTCTCCTGGc < 2:57217/275‑1 (MQ=255) gCTGGACGTTACCGCCAGCCAGCAGCTTGCTGATCAGTATCTTGATTTCGCCAGCCACGGTTTCCACGTTATCAGCGCCAACAAACTGGCGGGAGCCAGCGACAGCAATAAATATCGCCAGATCCACGACGCCTTCGAAAAAACCGGGCGTCACTGGCTGTACAATGCCACCGTCGGTGCGGGCTT‑GCCGATCAACCACACCGTGCGCGATCTGATCGACAGCGGCGATACTATTTTGTCGATCAGCGGGATCTTCTCCGGCACGCTCTCCTgg > 2:208414/1‑274 (MQ=255) cTGGACGTTACCGCCAGCCAGCAGCTTGCTGATCAGTATCTTGATTTCGCCAGCCACGGTTTCCACGTTATCAGCGCCAACAAACTGGCGGGAGCCAGCGACAGCAATAAATATCGCCAGATCCACGACGCCTTCGAAAAAACCGGGCGTCACTGGCTGTACAATACCACCGTCGGTGCGGGCTT‑GCCGATCAACCACACCGTGGGCGAACTGATCGACAGCGGCGATACTATTTTGTCCATACCCGGGAACTTCATCGGCACGCCCCCCTGGCt > 2:471669/1‑275 (MQ=255) tGGACGTTACCGCCAGCCAGCAGCTTGCTGATCAGTATCTTGATTTCGCCAGCCACGGTTTCCACGTTATCAGCGCCAACAAACTGGCGGGAGCCAGCGACAGCAATAAATATCGCCAGATCCACGACGCCTTCGAAAAACCCGGGCGTCACTGGCTGTACAATGCCACCGTCGGTGCGGGCtt > 4:18389/1‑184 (MQ=255) cGTTACCGCCAGCCAGCAGCTTGCTGATCAGTATCTTGATTTCGCCAGCCACGGTTTCCACGTTATCAGCGCCAACAAACTGGCGGGAGCCAGCGACAGCAATAAATATCGCCAGATCCACGACGCCTTCGAAAAACCCGGGCGTCACTGGCTGTACAATGCCACCGTCGGTGCGGGCTT‑GCCg < 2:18389/184‑1 (MQ=255) tACCGCCAGCCAGCAGCTTGCTGATCAGTATCTTGATTTCGCCAGCCACGGTTTCCACGTTATCAGCGCCAACAAACTGGCGGGAGCCAGCGACAGCAATAAATATCGCCAGATCCACGACGCCTTCGAAAAAACCGGGCGTCACTGGCTGTACAATGCCACCGTCGGTGCGGGCTT‑GCCGATCAACCACACCGTGCGCGATCTGATCGACAGCGGCGATACTATTTTGTCGATCAGCGGGATCTTCTCCGGCACGCTCTCCTGGCTGTTCCTGc > 2:199456/1‑275 (MQ=255) tACCGCCAGCCAGCAGCTTGCTGATCAGTATCTTGATTTCGCCAGCCACGGTTTCCACGTTATCAGCGCCAACAAACTGGCGGGAGCCAGCGACAGCAATAAATATCGCCAGATCCACGACGCCTTCGAAAAAACCGGGCGTCACTGGCTGTACAATGCCACCGTCGGTGCGGGCTT‑GCCGATCAACCACACCGTGCGCGATCTGATCGACAGCGGCGATACTATTTTGTCGATCAGCGGGATCTTCTCCGGCACGCTCTCCTGGCTGTTCCTGc > 2:262982/1‑275 (MQ=255) agccagcAGCTTGCTGATCAGTATCTTGATTTCGCCAGCCACGGTTTCCACGTTATCAGCGCCAACAAACTGGCGGGAGCCAGCGACAGCAATAAATATCGCCAGATCCACGACGCCTTCGACAAACCCGGGCGTCACNGGCTGTACAATGCCACCGTCGGTGCGGGCTT‑GCCGATCAACCACACCCTGCCCGGCCTGACCCACAGCCGCGACNCCATTTTCTCGACCAACCGGCTCTTCTCCCNNCCGCCCCCCTGGCTGTTCCTGCAACTCgcccnc > 3:67390/1‑274 (MQ=255) agccagcAGCTTGCTGATCAGTATCTTGATTTCGCCAGCCACGGTTTCCACGTTATCAGCGCCAACAAACTGGCGGGAGCCAGCGACAGCAATAAATATCGCCAGATCCACGACGCCTTCGAAAAAACCGGGCGTCACTGGCTGTACAATGCCACCGTCGGTGCGGGCTT‑GCCGATCAACCACACCGTGCGCGATCTGATCGACAGCGGCGATACTATTTTGTCGATCAGCGGGATCTTCTCCGGCACGCTCTCCTGGCTGTTCCTGCAATTCGa < 4:262982/275‑1 (MQ=255) cTTGCTGATCAGTATCTTGATTTCGCCAGCCACGGTTTCCACGTTATCAGCGCCAACAAACTGGCGGGAGCCAGCGACAGCAATAAATATCGCCAGATCCACGACGCCTTCGAAAAACCCGGGCGTCACTGGCTGTACAATGCCACCGTCGGTGCGGGCTT‑GCCGATCAACCACACCGTGCGCGATCTGATCGACAGCGGCGATACTATTTTGTCGATCAGCGGGATCTTCTCCGGCACGCTCTCCTGGCTGTTCCTGCAATTCGACGGTAGCGt < 2:564009/275‑1 (MQ=255) atataagagacaggATTTCGCCAGCCACGGTTTCCACGTTACCAGCGCCAACAAACTGGCGGGAGCCAGCGACAGCAATAAATATCGCCAGATCCACGACGCCTTCGAAAAACCCGGGCGTCACTGGCTGTACAATGCCACCGTCGGTGCGGGCTT‑GCCGATCAACCACACCGTGCGCGATCTGATCGACAGCGGCGATACTATTTTGTCGATCAGCGGGATCTTCTCCGGCACGCTCTCCTGGCTGTTCCTGCAATTCGACGGTAGCGTGCCGTTTAc < 1:82891/266‑1 (MQ=255) aGTATCTTGATTTCGCCAGCCACGGTTTCCACGTTATCAGCGCCAACAAACTGGCGGGAGCCAGCGACAGCAATAAATATCACCAGATCCACGACGCCTTCGAAAAAACCGGGCGTCACTGGCTGTACAATGCCACCGTCGGCGCGGGCTT‑GCCGCTCAACCACACCGTGCGCTATCTGATCCACAGCGGCCAACCTATTTTTTCGATTAGCGGCATCTTCGCCGGCACGCCCCCCTGCCTGTCCCTACCTCTCTGCTGTAGGATGCCGTTTAcc > 4:574220/1‑275 (MQ=255) aTCTTGATTTCGCCAGCCACGGTTTCCACGTTATCAGCGCCAACAAACTGGCGGGAGCCAGCGACAGCAATAAATATCGCCAGATCCACGACGCCTTCGAAAAAACCGGGCGTCACTGGCTGTACAATGCCACCGTCGGTGCGGGCTT‑GCCGATCAACCACACCGTGCGCGATCTGATCGACAGCGGCGATACTATTTTGTCGATCAGCGGGATCTTCTCCGGCACGCTCTCCTGGCTGTTCCTGCAATTCGACGGTAGCGTGCCGTTTACCGAg < 4:44819/275‑1 (MQ=255) cTTGATTTCGCCAGCCACGGTTTCCACGTTATCAGCGCCAACAAACTGGCCGGAGCCAGCGACAGCAATAAATATCGCCAGATCCACGGCGCCTTCGAAAAACCCGGGCGTCACGGGCTGTACAATGCCACCGTCGGTGCGGGCTT‑GCCGATCAACCACACCGTGCGCGATCTGATCGACAGCGGCGATACTATTTTGTCGATCAGCGGGATCTTCTCCGGCACGCTCTCCTGGCTGTTCCTGCAATTCGACGGTAGCGTGCCGTTTACCGAGCt < 2:563782/275‑1 (MQ=255) cTTGATGTCGCCAGCCACGGTTTCCACGTTATCAGCGCCAACAAACTGGCGGGAGCCAGCGACAGCAAGAAATATCGCCAGATCCACGACGCCTTCGAAAAACCCGGGCGTCACTGGCTGTACAATGCCACCGTCGGTGCGGGCTT‑GCCGATCAACCACACCGTGCGCGATCTGATCGACAGCGGCGATACTATTTTGTCGATCAGCGGGATCTTCTCCGGCACGCTCTCCTGGCTGTTCCTGCAATTCGACGGTAGCGTGCCGTTTACCGAGc < 2:353219/274‑1 (MQ=255) tGATTTCGCCAGCCACGGTTTCCACGTTATCAGCGCCAACAAACTGGCGGGAGCCAGCGACAGCAATAACTATCGCCAGATCCACGACGCCTTCGAAAAACCCGGGCGTCACTGGCTGTACAATGCCACCGTCGGTGCGGGCTT‑GCCGATCAACCACACCGTGCGCGATCTGATCGACAGCGGCGAAACTATTTTGTCGATCAGCGGGTTCTTCTCCGGCACGCTCTCCTGGCTGTTCCCGCAATTCAACGGTAGCGTGCCGTTTACCGGGCtgg > 2:124303/1‑275 (MQ=255) tCGCCAGCCACGGTTTCCACGTTATCAGCGCCAACAAACTGGCGGGAGCCAGCGACAGCAATAAATATCGCCAGATCCACGACGCCTTCGAAAAACCCGGGCGTCACTGGCTGTACAATGCCACCGTCGGTGCGGGCTT‑GCCGATCAACCACACCg > 2:599301/1‑156 (MQ=255) cagccaCGGTTTCCACGTTATCAGCGCCAACAAACTGGCGGGAGCCAGCGACAGCAATAAATATCGCCAGATCCACGACGCCTTCGAAAAACCCGGGCGTCACTGGCTGTACAATGCCACCGTCGGTGCGGGCTT‑GCCGATCAACCACACCGTgcg < 4:599301/156‑1 (MQ=255) ccaCGGTTTCCACGTTATCAGCGCCAACAAACTGGCGGGAGCCAGCGACAGCAATAAATATCGCCAGATCCACGACGCCTTCGAAAAACCCGGGCGTCACTGGCTGTACAATGCCACCGTCGGTGCGGGCTT‑GCCGATNAACCACACCGTGCGCGATCTGATCGACAGCGGCGATACTATTTTGTCGATCAGCGGGACCTTCTCCGGCCCGCCNCCCTCGCTGTTCCTCCACTTCCACGGTAGCNNGCCTTTTACCGATCtggtggctgcctcttttnc > 3:82891/1‑266 (MQ=255) ccaggTTTCCCTGTTATCAGCGCCAACAAACTGGCGTGAGCCAGCGACAGCAATAAATATCGCCAGATCCACGACGCCTTCGAAAAACCCGGGCGTCACTGGCTGTACAATGCCACCGTCGGTGCGGGCTT‑GCCGATCAACCACACCGTGCGCGATCTGATCGACAGCGGCGATACTATTTTGTCGATCAGCGGGATCTTCTCCGGCACGCTCTCCTGGCTGTTCCTGCAATTCGACGGTAGCGTGCCGTTTACCGAGCTGGAGGATCAGGCGTGgcag < 1:61811/276‑1 (MQ=255) gTTTCCCAGTTAGAAGCGCCAACAAACTGGCGGGGGCAAGCGACAGCAATAAAAATCGCCAGATCCACGCCGCCTTCGAAAAAACCGGGCGTCACTGGCTGTACAATGCCACCGTCGGTGCGGGCTT‑GCCGATCAACCACACCGTGCGCGATCTGATCGACAGCGGCGATACTATTTTGTCGATCAGCGGGATCTTCTCCGGCACGCTCTCCTGGCTGTTCCTGCAATTCGACGGTAGCGTGCCGTTTACCGAGCTGGTGGATCAGGCGTGgcag < 4:199456/275‑1 (MQ=255) tttCCACGTTATCAGGGACGACAAACTGGCGGGAGCCTGCTGCAGGAATAATACTCGCCAGATCCACGACGCCTTGGAGAAACCCGGTGGTCACTGGCTGTACAATGCCCCCGTCGGTGCGGGCTT‑GCCGATCAACCACACCGGGCGCGAACTGATCGACAGCGGCGAGACTATTTTGTCGATCAGCGGGATCTTCTCCGGCACGCTCTCCTGGCTGTTCCTGCAATTCGACGGTAGCGTGCCGTTTACCGAGCTGGTGGATCAGGCGTGgcagc < 4:194605/275‑1 (MQ=255) tCCACGTTATCAGCGCCAACAAACTGGCGGGAGCCAGCGACAGCAATAAATATCGCCAGATCCACGACGCCTTCGAAAAACCCGGGCGTCACTg > 4:500123/1‑94 (MQ=255) aCGTTATCAGCGCCAACAAACTGGCGGGAGCCAGCTACAGCAATAAATATCGCCAGATCCACGACGCCTTCGAAAAACCCGGGCGTCACTGGCTGTACAATGCCACCGTCGGTGCGGGCTT‑GCCGATCAACCGCACCGTGCGCGATCTGATCGACAGCGGCGATACTATTTTGTCGATCAGCGGGATCTTCTCCGGCACGCTCTCCTGGCTGTTCCTGCAATTCGACGGTAGCGTGCCGTTTACCGAGCTGGTGGATCAGGCGTGGCAGCAGGGc < 2:403235/275‑1 (MQ=255) cGTTATCAGCGCCAACAAACTGGCGGGAGCCAGCGACAGCAATAAATATCGCCAGATCCACGACGCCTTCGAAAAACCCGGGCGTCACTGGCTg < 2:500123/94‑1 (MQ=255) ccAACAAACTGGCGGGAGCCAGCGACAGCAATAAATATCGCCAGATCCACGACGCCTTCGAAAAACCCGGGCGTCACTGGCTGTACAATGCCACCGTCGGTGCGGGCTT‑GCCGATCAACCACACCGTGCGCGATCTGATCGACAGCGGCGATACTATTTTGTCGATCAGCGGGATCTTCTCCGGCACGCTCTCCTGGCTGTTCCTGCAACTCGACGGTACCGTCCCGTTTACCGCACTGGTGGTTCAGGCGTGGCAGGCGGGCTTAACCGAAccc > 4:175395/1‑274 (MQ=255) aCTGTCGGGAGCCAGCGACAGCAATAAATATAGCCAGATCCACGACGCCTTCGAAAAACCCGGGCGTCACTGGCTGTACAATGCCACCGTCGGTGCGGGCTT‑GCCGATCAACCAAACCGTGCGCGATCTGATCGACAGCGGCGATACTATTTTGTCGATCAGCGGGATCTTCTCCGGCACGCTCTCCTGGCTGTTCCTGCAATTCGACGGTAGCGTGCCGTTTACCGAGCTGGTGGATCAGGCGTGGCAGCAGGGCTTAACCGAACCTGACCcgc < 2:88267/275‑1 (MQ=255) cAGCGACAGCAATAAAAATCGCCAGATCCACGACGCCTTCGAAAAACCCGGGCGTCACTGGCTGTACAATGCCACCGTCGGTGCGGGCTT‑GCCGATCAACCACACCGTGCGCGATCTGATCGACAGCGGCGATACTATTTTGTCGATCAGCGGGATCTTCTCCGGCACGCTCTCCTGGCTGTTCCTGCAATTCGACGGTAGCGTGCCGTTTACCGAGCTGGTGGATCAGGCGTGGCAGCAGGGCTTAACCGAACCTGACCCGCGTGACGAtctct < 2:175395/275‑1 (MQ=255) cGACAGCAATAAATATCGCCAGAGCCACGACGCCTTCGAAAAACCCGGGCGTCACTGGCTGTACAATGCCACCGTCGGTGCGGGCTT‑GCCGATCAACCACACCGTGCGCGATCTGATCGACAGCGGCGATACTATTTTGTCGATCAGCGGGATCTTCTCCGGCACGCTCTCCTGGCTGTTCCTGCAATTCGACGGTAGCGTGCCGTTTACCGAGCTGGTGGATCAGGCGTGGCAGCAGGGCTTAACCGAACCTGACCCGCGTGACGATCTCTCTGGCa < 1:67390/278‑1 (MQ=255) ttaagagaacggtCCACAACGCCGTGGAAAAACCCGGCGGTCACTGGCTGTACAATGCCACCGTCGGTGCGGGCTT‑GCCGATCAACCACACCTGGCGCGATCGGATCGACAGCGGCGATACTATTTTGTCGATCAGCGGGATCTTCTCCGGCACGCTCTCCTGGCTGTTCCTGCAATTCGACGGTAGCGTGCCGTTTACCGAGCTGGTGGATCAGGCGTGGCAGCAGGGCGTAACCGAACCTGACCCGCGTGACGATCTCTCTGGCAAAGACGt < 4:167367/262‑1 (MQ=255) atCGCCAGATCCACGACGCCTTCGAAAAAACCGGGCGTCACTGGCTGTACAATGCCACCGTCGGTGCGGGCtt > 4:627409/1‑73 (MQ=255) cGCCAGATCCACGACGCCTTCGAAAAACCCGGGCGTCACTGGCTGTACAATGCCACCGTCGGTGCGGGCTT‑GCCGATCAACCACACCGTGCGCGATCTGATCGACAGCGGCGATACTATTTTGTCGATCAGCGGGATCTTCTCCGGCACGCTCTCCTGGCTGTTCCTGCAATTCGACGGTAGCGTGCCGTTTACCGAGCTGGTGGATCAGGCGTGGCAGCAGGGCTTAACCGAACCTGACCCGCGCGACACTCTCTCTGGCAAAGACGTGATg > 2:282651/1‑273 (MQ=255) ccAGATCCACGACGCCTTCGAAAAAACCGGGCGTCACTGGCTGTACAATGCCACCGTCGGTGCGGGCTT‑GCCg < 2:627409/73‑1 (MQ=255) gacccACGACGCCTTCGAAAAAACCGGGCGTCACTGGCTGTACAAGGCCACCGTCGGGGCGGGCTT‑GCCGATCAACCCCACCGTGCGCGATCTGATCGACAGCGGCGATACTATTTTGTCGATCAGCGGGATCTTCTCCGGCACGCTCTCCTGGCTGTTCCTGCAATTCGACGGTAGCGTGCCGTTTACCGAGCTGGTGGATCAGGCGTGGCAGCAGGGCTTAACCGAACCTGACCCGCGTGACGATCTCTCTGGCAAAGACGTGATGCGCaa < 2:407126/270‑1 (MQ=255) ccTTCGAAAAACCCGGGCGTCACTGGCTGTACAATGCCACCGTCGGTGCGGGCTT‑GCCGATCAACCACACCGTGCGCGATCTGATCGACAGCGGCGATACTATTTTGTCGATCAGCGGGATCTTCTCCGGCACGCTCTCCTGGCTGTTCCTGCAATTCGACGGTAGCGTGCCGTTTACCGAGCTGGTGGATCAGGCGTGGCAGCAGGGCTTAACCGAACCTGACCCGCGTGACGATCTCTCTGGCAAAGACGTGATGCGCAAGCTGGTGATTCTg > 2:365783/1‑275 (MQ=255) ccTTCGAAAAACCCGGGCGTCACTGGCTGTACAATGCCACCGTCGGTGCGGGCTT‑GCCGATCAACCACACCGTGCGCGATCTGATCGACAGCGGCGATACTATTTTGTCGATCAGCGGGATCTTCTCCGGCACGCTCTCCTGGCTGTTCCTGCAATTCGACGGTAGCGTGCCGTTTACCGAGCTGGTGGATCAGGCGTGGCAGCAGGGCTTAACCGAACCTGACCCGCGTGACGATCTCCCTGGCAAAGCCGTGATGAGAAAGCTGGTGATTCTGgcgc > 1:138127/1‑279 (MQ=255) cTTCGAAAAACCCGGGCGTCACTGGCTGTACAATGCCACCGTCGGTGCGGGCTT‑GCCGATCAACCACACCGTGCGCGATCTGATCGACAGCGGCGATACTATTTTGTCGATCAGCGGGATCTTCTCCGGCACGCTCTCCTGGCTGTTCCTGCAATTCGACGGTAGCGTGCCGTTTACCGAGCTGGTGGATCAGGCGTGGCAGCAGGGCTTAACCGAACCTGACCCGCGTGACGATCTCTCTGGCAAAg > 2:167367/1‑248 (MQ=255) cTTCGAAAAACCCGGGCGTCACTGGCTGTACAATGCCACCGTCGGTGCGGGCTT‑GCCGATCAACCACACCGTGCGCGATCTGATCGACAGCGGCGATACTATTTTGTCGATCAGCGGGATCTTCTCCGGCACGCTCGCCTGGCTGTTCCTGCAATTCGACGGTAGCGTGCCGATTACCGAGCTGGTGGATCAGGCGTGGCAGCAGGGCTTAACCGAACCTGACCCGAGTGAACATCTCTCTGGCAAAGACGTGATTCGCAAGCTGGTGATTCCgg > 4:343400/1‑275 (MQ=255) cTTAGAAAAAACCGGGGGTCACTGGCTGTACAATGCCACGGTCGGTGCGGGCGT‑GACGATCAACCACACCGTGCGCGATCTGATCGACAGCGGCGATACTATTTTGTCGATCAGCGGGATCTTCTCCGGCACGCTCTCCTGGCTGTTCCTGCAATTCGACGGTAGCGTGCCGTTTACCGAGCTGGTGGATCAGGCGTGGCAGCAGGGCTTAACCGAACCTGACCCGCGTGACGATCTCTCTGGCAAAGACGTGATGCGCAAGCTGGTGATTCTg < 2:423479/274‑1 (MQ=255) gAAAAACCCGGGCGTCACTGGCTGTACAATGCCACCGTCGGTGCGGGCTG‑GCCGATCAACCACACCGTGCGCGATCTGATCGACAGCGGCGATACTATTTTGTCGATCAGCGGGATCTTCTCCGGCACGCTCTCCTGGCTGTTCCTGCAATTCGACGGTAGCGTGCCGTTTACCGAGCTGGTGGATCAGGCGTGGCAGCAGGGCTTAACCGAACCTGACCCGCGTGACGATCTCTCTGGCAAAGACGTGATGCGCAAGCTgg < 2:343400/262‑1 (MQ=255) aCCGGGCGTCACTGGCTGTACAATGCCACCGTCGGTGCGGGCTT‑GCCGATCAACCACACCGTGCGCGATCTGATCGACAGCGGCGATACTATTTTGTCGATCAGCGGGATCTTCTCCGGCACGCTCTCCTGGCTGTTCCTGCAATTCGACGGTAGCGTGCCGTTTACCGAGCTGGTGGATCAGGCGTGGCCGCAGGGCTTAACCGAACCTGACCCGCGGTGCGATTCCCCTGGCCAAGACGGTACTCGCAAACCGGTGATTCTGGGCCGAGAAGc > 4:30465/1‑275 (MQ=255) | TTGAGCTATGACGGGCTGGACGCCAGCCGCGCGTTAGCCTTCTTCAACGATGAAGCGGTTGAGCAGGATGAAGAGTCGTTGTTCCTGTGGATGCGCGCCCATCCGTATGATGATTTAGTGGTGCTGGACGTTACCGCCAGCCAGCAGCTTGCTGATCAGTATCTTGATTTCGCCAGCCACGGTTTCCACGTTATCAGCGCCAACAAACTGGCGGGAGCCAGCGACAGCAATAAATATCGCCAGATCCACGACGCCTTCGAAAAAACCGGGCGTCACTGGCTGTACAATGCCACCGTCGGTGCGGGCTT‑GCCGATCAACCACACCGTGCGCGATCTGATCGACAGCGGCGATACTATTTTGTCGATCAGCGGGATCTTCTCCGGCACGCTCTCCTGGCTGTTCCTGCAATTCGACGGTAGCGTGCCGTTTACCGAGCTGGTGGATCAGGCGTGGCAGCAGGGCTTAACCGAACCTGACCCGCGTGACGATCTCTCTGGCAAAGACGTGATGCGCAAGCTGGTGATTCTGGCGCGTGAAGC > NC_000913/4131347‑4131885 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |