| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 4,183,165 | G→A | 100% | E641K (GAA→AAA) | rpoB → | RNA polymerase, beta subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,183,165 | 0 | G | A | 97.8% | 320.1 / ‑2.3 | 97 | E641K (GAA→AAA) | rpoB | RNA polymerase, beta subunit |
| Reads supporting (aligned to +/- strand): ref base G (1/2); new base A (48/46); total (49/48) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.17e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCC‑GTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACAT‑GGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTT‑CCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGTACGTGGAT > NC_000913/4182904‑4183428 | catgaCGGTCGCGGATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACGCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCC‑GTATCGTCAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCAAATCCAGCTTGTTCAGc < 1:83171/275‑1 (MQ=255)cACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCC‑GTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACCCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCAAATCCAGCTTGtt > 2:454277/1‑275 (MQ=255) aCGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCC‑GTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAg > 2:264976/1‑274 (MQ=255) tCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCCCAGACTAACGAATACGGCTTCCTTGAGACTCC‑GTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTACACCTTGGATGAAGAAGGCCACTTCGTAAAAGCCCTGGTACCTTGCCGTAGCAAAGGCAAATCCAGCTTGTTCAGCcccg > 4:338217/1‑275 (MQ=255) gcgTATGGACACTCTAAACCCCTGAAGGTCCGAACATCGGTCTTATCAACTCTCTGTACGTGTACGCACAGACTAACTACTACGGCTTCCTTGCGACTCC‑GTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAATAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGAc < 4:120264/275‑1 (MQ=255) cgTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGCGACTCC‑GTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTTGCTGAATAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCAAATCCATCTTGTTCAGCCGCGAcc > 2:163031/1‑275 (MQ=255) cgTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCC‑GTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGACGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAAAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCAAATCCCGCTTGTTCACCAGCAAcc > 4:123674/1‑275 (MQ=255) cgTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCC‑GTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCATCTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCAAATCCAGCTTGTTCAGCCGCGAcc > 2:381141/1‑275 (MQ=255) gTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCC‑GTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCAAATCCAGCTTGTTCAGCCGCGACCAGGtt < 2:126752/275‑1 (MQ=255) cAATCGAAACCGCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCC‑GTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCAAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTAc < 1:122916/278‑1 (MQ=255) aaTCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAGTACGGCTTCCTTGAGACTCC‑GTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCAAATCCAGCTTGTTCAGCCGCGACCAGGTTGAc > 2:106937/1‑274 (MQ=255) aaCCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCC‑GTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCAAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACAT‑g < 2:194683/274‑1 (MQ=255) aCCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCC‑GTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCAGCTCTCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTGCGGAGAAGACCTGGTAACTTGCCGTACCAAAGGCAAATCCAGCTTGTTCAGCCGCGACCAAGTTGACTAccttg > 4:455757/1‑270 (MQ=255) tGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCC‑GTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCAAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACAT‑GGACGTAt > 4:460018/1‑275 (MQ=255) ccGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTCAGACTCC‑GTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCNACTACGTTATAGCCCAGGCGAACTCCAACTGGGATGAAGAAGGCCACTTCGTAGAAGACCTGGCAACTTGCCGTNGCACAGGCAAATCCAGCTTGTTCAGCGGCCNNCAGGTTGACTCAAT‑GGCAGTACCCACTcacc > 3:69014/1‑275 (MQ=255) cGAACATCGGTCTGAGCAGCACTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCC‑GTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATGCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCAAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACAT‑GGACGTATCCACCcag < 2:455757/275‑1 (MQ=255) gaaatacGGTCTGATCAAAGTACTGGCCGTGTACGAACAGACTAACGATTACGGCTTCCTTGGGAATCC‑GTAACGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTGATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCAAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACAT‑GGACGTATCCACCcagc < 4:515852/269‑1 (MQ=255) aaCATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCC‑GTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCAAATCCAGCTTGTTCAGCCGCGACCAgg > 2:577505/1‑247 (MQ=255) tCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCC‑GTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCAAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACAT‑GGACGTATCCACCCAGCAGGTGGTATCCGTCGGTg < 1:45896/279‑1 (MQ=255) aaCGCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCC‑GTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCAAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACAT‑GGACGTATCCACCCAGCAGGTGGTATCCGTCg < 2:118659/274‑1 (MQ=255) aCTCTCTGTCCCTGTACTCACAGCATAACGAATACGGTTTCCTTGAGACTCC‑GTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTGATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCAAATCCAGCTTGTTCAGCCGCGACCAGTTTGACTACAT‑GGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGt < 1:59467/279‑1 (MQ=255) gcttctGTCCGTGTACCGACAGACTTACGAAGGCGGGTTCCATGAGGCTCC‑GCATCGTAAAGGGAGCGAGGGGGTTGTGACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGAAATTAACGTATCGCCCAGGACAACTCCAAAGTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCAGATCCAGCTGGTTCAGCCGCGACCAGGTTGACTACAT‑GGACGTATCCACCCAGCAGGTGGTATCCGTCGGTg < 4:29746/272‑1 (MQ=255) ctctctGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCC‑GTAACGTAAAGTGAACGACGGTGTTGTCACTGACGATATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACCCCAACTTGGATGAATGTGTCCACTTCGTAGACGACCTGGTACCCTTGCGTAGCAACGGCAAATACCGCTTGTTCAGCCACGACCAGGTTGACTTCAT‑GGACGTATCCCCCCAGCCGGTGTTATCCCTCGGt > 4:76046/1‑274 (MQ=255) gtcgttCCGTGTACGCACAGACTAACGAATACGGCTTCCTGGAGAGTCCTGTA‑CGTAAAGTGACCGACGGGGTTGTAACTGACGAAATTCACAACCTGTCTGCTATCGAAGAAGGCAACTGCGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGGCCTGGTAACTTGCCGTAGCAAAGGCAAAGCCAGCTTGTTCAGCCGCGACCAGGTTGACTACAT‑GGACGGATCCACCCAGCAGGTGGTATCCGTCGGTGCg < 4:100336/270‑1 (MQ=255) tGTCCGTGTACGCACATACTAACGAATACGGCTTCCTTGAGACTCC‑GTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCAAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACAT‑GGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCg > 2:10741/1‑272 (MQ=255) tgttctcacaGACTAACGAATACGGCTTCCTTGAGACTCC‑GTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCAAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACAT‑GGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGt < 1:151363/273‑1 (MQ=255) tACGCACAGACTAACGAATACGGCTTCCTTGAGACTCC‑GTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTACCGTAGCAACGGCAAATCCAGCTTGTTCAGCCGCGACCAGTTTGACTCCAT‑GCACGTATCCACCCAGCGGGTGGTATCCGCCGGTTCACCCCTGATcc > 4:38045/1‑274 (MQ=255) cacaGACTAACGAATACGGCTTCCTTGAGACTCC‑GTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCAAATCCAGCTTGTTCAGCCGCGACCAGGTTGCCTAAAT‑GGACGTATCCACCCAGCAGGTGGTAGCCGTCGGTGCCTCCCGGATCCCGTT‑c > 4:50623/1‑275 (MQ=255) aCTAACGAATACGGCTTCCTTGAGACTCC‑GTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCAAATCCCGCTTGTTCAGCCGCGACCAGGTTGACTACCT‑GGACGTATCAACCCAGCAGGTGGTATCCCGGGGTGTGTCCCTGAACCCGTT‑ccggtg > 4:501314/1‑271 (MQ=255) aCGAATACGGCTTCCTTGAGACTCC‑GTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCAAATCCAGCTTGTTCAGCcg > 4:619370/1‑196 (MQ=255) aTACGGCTTCCTTGAGACTCC‑GTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCAAATCCAGCTTGTTCAGCCGCGAc < 2:619370/196‑1 (MQ=255) aCGGCTTCCTTGAGACTCC‑GTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCTAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGCTGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCAAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACAT‑GGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTT‑CCTGGAACACGATGAc > 2:60567/1‑275 (MQ=255) aCGGCTTCCTTGAGACTCC‑GTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCAAATCCAGCTTGTTCAGCcg > 4:608278/1‑190 (MQ=255) aCGGCTTCCTTGAGACTCC‑GTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCAAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACAT‑GGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTT‑CCTGGAACACGATGAc > 2:578087/1‑275 (MQ=255) tttCCTGTAGACTCC‑GTATAGTGAAGGAGCCNNGGGTGTTGTAACTTGCGAAATTCACTAACTNTCTACTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAAATTGGATAAGGAAGGCCACTTCGTAGANGACCTGGTAACTTGCCGTAGCAAAGGCAAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACAT‑GGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTT‑CCTGGAACACGATGACGCCAAc < 3:66387/276‑1 (MQ=255) cTTCCTTGCGACTCC‑GTATCGTAAAGTGACCCACGGTGTTTTAACTGACGAAACTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCACCCAGGCGAACCCCAACTTGGTTGAAGACGCCGACTTCGTAGCAGACCTGGTAACTTGCCGTAGCCAAGGCAACTACAGCTTGTTCAGCCTCGATCAGGTCTACCATAT‑GGACGTATCTACCCAGCAGATCGTCTCCGTCGCTGCGTCCCTTAACCCCTT‑TCTGCAACTCGCTGAcccc > 4:323994/1‑274 (MQ=255) cTTCCTTGAGACTCC‑GTATCGTAAAGTGACCGCCGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTACCGAAGAAGGCAACTACGTTATCGCCCAGGCGAAATCCAACTTGGATGAAGAAGGCCACTTCGTAGCAGACCTGGTAACTTACAGTAGCCATGCAAAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTCCAT‑CGACTACACCACCCAGCAGGAGGTCTGCGTAGGTGGGACCCTGAACCCGTTACCTGGacacgctagcagac > 4:63555/1‑262 (MQ=255) cTTCCTTGAGACTCC‑GTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCAAATCCAGCTTGTTCAGCCGCGAc < 2:608278/190‑1 (MQ=255) cTTCCTTGAGACTCC‑GTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCAAATCCAGCTTGTTCAGCCGCGACAAGGTTGACTACAT‑GGACGTATCCACCCAGCAGGTTGTATCCGTCGGTGCGTCCCTGATCCCGTT‑CCTGGAACACGATGACGCCa > 2:572689/1‑275 (MQ=255) agaCTCC‑GTATCGTAAAGTGACCGACGGTATTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCAAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACAT‑GGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTT‑CCTGGAACACGATGACGCCAACCGTGc < 2:149215/274‑1 (MQ=255) agaaacagaTCTTAAATGGACCGACGGTGTTTTAACTGACCAAATTCAATACCTGTCTGCTATCGAAGAAGGAAACGACGTNATCGCCCAGGCGAACGCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCAAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACAT‑GGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTT‑CCTGGAACACGATGACGCCAACCGTGCATTGAt < 3:150485/271‑1 (MQ=255) aCTCC‑GTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTANCTTACCGTAGCAAATGCAAATCCAGCTTGTTCAGCCGCGCCCAGTTGGCTTCCAT‑GGACGACTCCCCACCGCAGNGGGTCTCCGTAGGAACGGCACGGATCCCGTN‑NCCGGCACACAGTGACGCCAAcgggtcctgatng > 3:73493/1‑267 (MQ=255) cc‑GTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCAAATCCAGCTTGTTCAGCCGCGACCAGGtt > 1:158060/1‑183 (MQ=255) cc‑GTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCAAATCCAGCTTGTTCAGCCGCGACCAGGtt < 3:158060/183‑1 (MQ=255) c‑GTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCAAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACAT‑GGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTT‑CCTGGAACACGATGACTCCACCCGTCCCTTTATg > 4:461568/1‑275 (MQ=255) c‑GTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCAAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACAT‑GGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTT‑CCTGGAACACGATGACGCCAACCGTGCATTGATg > 2:468107/1‑275 (MQ=255) c‑GTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCAAATCCAGCTTGTTCAGCCGCAACCAGGTTGACTACAT‑GGACGTATCCACCCAGCAGGTGGTATCCTTCGGTGCGTCCCTGATCCCGAT‑CCTGGGAAACGATGAACCCAACAGTGCATTGAt > 2:349198/1‑274 (MQ=255) gTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCAAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACAT‑GGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTT‑CCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCg < 2:460018/275‑1 (MQ=255) tgcccgccgggtttgtAACTTACGAAATTCACTACCTGTCTGCGATCGAAGAAGGCAACTTCGTTATCGCCCAGGCGAACTCCAACCTGGATGAAGAAGGCCACTTCGTTGAAGACCTGGTAACTTGCCGTAGCAAAGGCAAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACAT‑GGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTT‑CCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATg < 4:431129/263‑1 (MQ=255) aCCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCAAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACAT‑GGACGTATCCACCCAGCAGGTGGTATCCGTCGTTGCGTCCCTGATCCCGTT‑CCTGGAACACGAGGACGCCAACCGTGCATTGATGGGTGCGAACATGc > 2:161537/1‑274 (MQ=255) aCCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCAAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACAT‑GGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTT‑CCTgg > 4:419873/1‑232 (MQ=255) aCCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCAAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACAT‑GGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTT‑CCTGGAACACGCTGACGCCAACCGTGCATTGATGGGTGCGAACactgtct > 1:150485/1‑271 (MQ=255) aCGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCAAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACAT‑GGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTT‑CCTGGAaca < 2:419873/232‑1 (MQ=255) tgttgtAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCAAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACAT‑GGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTT‑CCTGGAaca > 2:483160/1‑228 (MQ=255) gttttAACTGACGAAATTCCCTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAATGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCAAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACAT‑GGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTT‑CCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGc < 2:50623/275‑1 (MQ=255) gttgtAACTGACGAAATTCACTACCTGACTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAAAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCAAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACAT‑GGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTTCCTGATCCCGTT‑CCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGc < 2:123674/275‑1 (MQ=255) gttgtAACGGACGAAATTGCCTACCTGGCGGCTATCGAAGAAGGCAACCACGTAATCGCCAAGGCGAAGTCAACATGGGAGGAGGAAGGCAACTTGTTAGAAGCCCGGGTAACTTGCCGTAGCAAAGGCAAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACAT‑GGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTT‑CCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGc < 4:208231/275‑1 (MQ=255) gtAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCAAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACAT‑GGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTT‑CCTGGAACACGAt < 4:483160/228‑1 (MQ=255) gACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCAAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACAT‑GGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTT‑CCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGAc > 2:523166/1‑275 (MQ=255) cGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCAAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACAT‑GGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTT‑CCTGGACCACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAAACTCAGGCCGTTccaccc > 4:398984/1‑270 (MQ=255) gtCACTACCTGTCTGCTATCGAGGAAGGCAACGCCGTGATCGCCCAGGCGAACTCCAACTTGGAGGAAGAAGGTCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCAAATCCGGCTGGTTCAGCCGCGACCAGGTTGACTACAT‑GGACGTATCCACCCAGCCGGTGGTATCCGTCGTTGCGTCCCTGATCCCGTT‑CCTGGAACACGAGGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTgcgc < 4:301750/274‑1 (MQ=255) gTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCAAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACAT‑GGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTT‑CCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATa > 2:249833/1‑272 (MQ=255) cTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCAAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACAT‑GGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTT‑CCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCg < 2:338217/275‑1 (MQ=255) cTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCAAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACAT‑GGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTT‑CCTGGAACACGATGACGCCAACCGTGCATTGATGGTTGCGAACATGCAACGTCAGGCCGTTCCACTTCTGCGCGCAGATACGGCGCccgttg > 1:174950/1‑279 (MQ=255) aTCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCAAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACAT‑GGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTT‑CCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGActc > 2:213919/1‑253 (MQ=255) aTCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCAAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACAT‑GGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTT‑CCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCtggt < 2:501314/275‑1 (MQ=255) aTCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGGAACTTGCCGTAGCAAAGGCAAATCCAGCTTGTTCAGCCGCGACCAGATTGACTACAT‑GGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTT‑CCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCtggt < 2:50845/275‑1 (MQ=255) aTCGAAAAAGGCAAATACGTTATCGACCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCGGGTAACTTGCCGTAGCAAAGGCAAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACAT‑GGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTT‑CCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCtggt < 4:468107/275‑1 (MQ=255) cgcatcacgCAACTACGTTATCGCCCCGGCTAACTCCAACTTGGAGGATGAAGGCCACTTCGTAGAATACCTGGTAACTTGCCGTAGCAAAGGCAAACCCAGCTTGTTCAGCCGCGACCAGGTTGACTACAT‑GGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGTTCCCGTT‑CCTGGACCACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTAc < 1:73493/271‑1 (MQ=255) gagcaagCCACCTACGTAAGAGCGCAGGCAAACTTCGTCTTGGAGAAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGGAGCAAAGGCAAATCCTGCGTGTTCAGCCGCGACCAGGTTGGTGACAT‑GGACGTATCCACCCAGCAGGTGTTATCCGTCGGTGCGTCCCTGATCCCGTT‑CCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCtggttgg < 4:381141/271‑1 (MQ=255) aagaagGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCAAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACAT‑GGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTT‑CCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTgcg < 4:213919/253‑1 (MQ=255) gcaaggaaatgccGTTAACGCCCAGGCGAACCCCACCTTGGATGAAGAAGGCCACTTCGGAGAAGACCGGGAAACTTGCCGTAGCAAAGGCAAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACAT‑GGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTT‑CCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTa < 4:423081/263‑1 (MQ=255) agaagGCAACTACGTTATCGCCCAGGCTAACTCCAACTTGAATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCAAATCCAGCTTGGTCAGCCGCGACCAGGTTGACTACAT‑GGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTT‑CCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTACGAATCTGCGCGCTGATAAGCCGCTGGTTGGTa < 2:106736/275‑1 (MQ=255) agaagGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCAAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACAT‑GGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTT‑CCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTa < 4:249833/275‑1 (MQ=255) gaagGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCAAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACAT‑GGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTT‑CCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTgcg < 4:223101/251‑1 (MQ=255) gaagGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCAAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACAT‑GGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTT‑CCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTgcg > 2:223101/1‑251 (MQ=255) tCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCAAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACAT‑GGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTT‑CCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTg < 4:10741/273‑1 (MQ=255) cAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCAAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACAT‑GGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTT‑CCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGc > 2:26946/1‑275 (MQ=255) ggCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCAAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACAT‑GGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTT‑CCTGGAACACGATGACGCCATCCGTGCATTGATGGGTGCGAACATGCACCGTCCTGCCGTTCCGACCCTGCGCGCTGCTAATCCGCTGGTTGGGACTGGTTTTGAGCGTTCGTTTGCCg > 4:565011/1‑275 (MQ=255) cTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCAAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACAT‑GGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTT‑CCTGGAACACGATGACGCCACCCGTGCATTGACGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTACGCGCTCATAAGCCGCTGCTTGGTACTGGTATGGACCGTTCCGTTGCCGtcaact > 4:44216/1‑270 (MQ=255) gaggaagaaGGCCACTTCGTAGAAGACCTGGTCACGTGCCGTAGAAAAGGCAAATCCAGGTTGTTCAGCCGCGGCCAGGTTGACTACAT‑GGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTT‑CCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCg < 1:39384/276‑1 (MQ=255) aTGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCAAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACAT‑GGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTT‑CCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACt > 2:92834/1‑275 (MQ=255) gaagGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCAAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACAT‑GGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTT‑CCTGGAACACGATGACGCNAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTCCCGAccnng > 4:599089/1‑200 (MQ=255) gTAGAAGACTTGGAAACTGGCCGTAGCAAACGCAGATCCACGTTGTTCACCCGCGCCACGGTTGACTACAT‑GGAGGTATCCACCCAGCAGGTGGTATCCGGCGGTGCGTCCCTGCGCCCGTT‑CCTGGACCACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACgtgg < 4:26946/275‑1 (MQ=255) agaagaCCTGGTAACTTGCCGTAGCAAAGGCAAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACAT‑GGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTT‑CCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTTCCTCCGGTGTCAATGCGGTAGCTAAACGGAGTGgtgt > 1:84698/1‑279 (MQ=255) aCCTGGTAACTTGCCGTAGCAAAGGCAAATCCAGCTTGTTCAGCCGCGACCAGGTTGCCTACAT‑GAACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTT‑CCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAAAATGCAACGTCCGGCCGTTCAGACTCTTCGCGATGATAAGCCGCTTGTTGGTACTGGTATGGACAGTGCTGTTGCCGTTGACTCCGGTGTAATCGCGGTACATAAACGAGGGGGTGTc > 4:228189/1‑275 (MQ=255) cTGGTAACTTGCCGTAGCAAAGGCAAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACAT‑GGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTT‑CCTGGAACACGATGACGCCAACCGTGCATTGATGGGGGCGAACCTGCAACGTCAGGCCGTTCCGACCCCGCCCTCCGATAAGCCNCTGGTTGGGACTGGTATGGACCGCTCTGTTGCCGGTGACCCCGGTGTAACTTCGGTAGCTAAACgtggttgcggtggttcg > 3:147187/1‑267 (MQ=255) tGGTAACTTGCCGTAGCAAAGGCAAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACAT‑GGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTT‑CCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGtt < 2:565011/275‑1 (MQ=255) ggTAACTTGCCGTAGCAAAGGCAAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACAT‑GGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTT‑CCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCtggttg > 2:469999/1‑203 (MQ=255) ggTAACTTGCCGTAGCAAAGGCAAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACAT‑GGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTT‑CCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGaa > 4:317325/1‑217 (MQ=255) acattGCCGTAGCAAAGGCAAATCCAGCTTGTTCGGCCGCGACCAGGTTGACTACAT‑TGACCTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTT‑CCTGGAACAGGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAg < 4:106937/271‑1 (MQ=255) aCTTGCCGTAGCAAAGGCAAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACAT‑GGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTT‑CCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTAc < 4:469999/203‑1 (MQ=255) aCTTGCCGTAGCAAAGGCAAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACAT‑GGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTT‑CCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTg < 2:317325/217‑1 (MQ=255) gCCGTAGCAAAGGCAAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACAT‑GGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTT‑CCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCtggtt > 4:375650/1‑194 (MQ=255) gTAGCAAAGGCAAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACAT‑GGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTT‑CCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCCGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCt < 2:97752/245‑1 (MQ=255) ctAGCAAAGGCGAAACACGCGTGTTCGGCCGCCACCGAGTTGAC‑GCATCGGACGACTGCACACACCAGGTGGGATCGGTCGGTGGGTCCCTGATCCCGGT‑CCTGGAACACGAGGACGCGAACCGGGCATTGATTGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCGGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGGGCTGTTGCCGTTGACGCCGGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGTACGTGGAt < 4:264976/274‑1 (MQ=255) tAGCAAAGGCAAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACAT‑GGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTT‑CCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTa < 2:375650/194‑1 (MQ=255) | CACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCC‑GTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACAT‑GGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTT‑CCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGTACGTGGAT > NC_000913/4182904‑4183428 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |