| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 375,045 | G→A | 6.8% | intergenic (+164/‑414) | mhpE → / → mhpT | 4‑hyroxy‑2‑oxovalerate/4‑hydroxy‑2‑oxopentanoic acid aldolase, class I/3‑hydroxyphenylpropionic transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 375,045 | 0 | G | A | 6.8% | 193.7 / 3.9 | 59 | intergenic (+164/‑414) | mhpE/mhpT | 4‑hyroxy‑2‑oxovalerate/4‑hydroxy‑2‑oxopentanoic acid aldolase, class I/3‑hydroxyphenylpropionic transporter |
| Reads supporting (aligned to +/- strand): ref base G (24/31); new base A (2/2); total (26/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.64e-01 | |||||||||||
GTGCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTT‑AGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGA > NC_000913/374899‑375192 | gTGCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTT‑AGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGaa > 2:68066/1‑149 (MQ=255) aTTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTT‑AGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGcc < 2:196210/149‑1 (MQ=255) ttttGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTT‑AGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCt < 2:29494/149‑1 (MQ=255) ttttGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTT‑AGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCt > 2:1019514/1‑149 (MQ=255) cccATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTT‑AGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGcc > 3:383512/1‑149 (MQ=255) aTCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTT‑AGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTAc < 1:1019514/149‑1 (MQ=255) tCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTT‑AGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACg > 1:592792/1‑149 (MQ=40) cAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTT‑AGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGAt < 1:68066/149‑1 (MQ=40) aCGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTT‑AGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGgcg > 2:118721/1‑149 (MQ=39) gACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTT‑AGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGcgcg < 3:392508/149‑1 (MQ=39) aCTGCCGGAGGCGGCGTGAACGCCTTATCCCGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTT‑AGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCgg < 2:247062/149‑1 (MQ=255) cTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTT‑AGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCggg < 4:1130949/149‑1 (MQ=40) ggATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTT‑AGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAAttt < 2:592792/149‑1 (MQ=255) aTGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTT‑AGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGt > 1:774231/1‑149 (MQ=40) aTGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTT‑AGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGt < 4:383512/149‑1 (MQ=39) tGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTT‑AGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTa < 3:630631/149‑1 (MQ=39) tGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTT‑AGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTa > 3:530387/1‑149 (MQ=39) tGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTT‑AGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTa < 3:350407/149‑1 (MQ=40) tGAACGCCTTATTCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTT‑AGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGa < 1:633163/149‑1 (MQ=255) gAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTT‑AGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGAt < 1:118721/149‑1 (MQ=40) aTCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTT‑AGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGt < 1:361498/149‑1 (MQ=39) ccGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTT‑AGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTa < 2:774231/149‑1 (MQ=40) ggCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTT‑AGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGcc < 1:951676/95‑1 (MQ=40) ggCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTT‑AGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGcc > 2:951676/1‑95 (MQ=40) cTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTT‑AGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGCCCTGATa > 1:101011/1‑134 (MQ=255) cTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTT‑AGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGCCCTGATa < 2:101011/134‑1 (MQ=255) ggATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTT‑AGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATc > 2:950312/1‑93 (MQ=255) ggATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTT‑AGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATc < 1:950312/93‑1 (MQ=255) gATGGCGTAAGAATTTGTAGGTCTGATAAGACGCGTT‑AGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATc > 3:344327/1‑149 (MQ=255) aTGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTT‑AGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCa < 4:267288/149‑1 (MQ=38) gCGTGAGAATTTGTAGGTCTGATAAGACGCGTT‑AGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGc > 1:968303/1‑149 (MQ=37) tGAGAATTTGTAGGTCTGATAAGACGCGTT‑AGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTAc > 2:630330/1‑93 (MQ=37) tGAGAATTTGTAGGTCTGATAAGACGCGTT‑AGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTAc < 1:630330/93‑1 (MQ=37) tGAGAATTTGTAGGTCTGATAAGACGCGTT‑AGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAg < 4:60102/144‑1 (MQ=35) tGAGAATTTGTAGGTCTGATAAGACGCGTT‑AGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAg > 3:60102/1‑144 (MQ=35) agaATTTGTAGGTCTGATAAGACGCGTT‑AGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTa > 2:553250/1‑111 (MQ=35) agaATTTGTAGGTCTGATAAGACGCGTT‑AGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTa < 1:553250/111‑1 (MQ=35) aGGTCTGATAAGACGCGTT‑AGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACt < 2:968303/149‑1 (MQ=37) ggTCTGATAAGACGCGTT‑AGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTg > 2:452348/1‑149 (MQ=37) aTAAGACGCGTT‑AGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGcc > 2:1117671/1‑145 (MQ=34) aTAAGACGCGTT‑AGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGcc < 1:1117671/145‑1 (MQ=34) aGACGCGTTAAGCGTCGCATCAGGCGTCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATgc < 1:826432/149‑1 (MQ=255) aGACGCGTT‑AGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGcc > 1:464366/1‑96 (MQ=34) aGACGCGTT‑AGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGcc < 2:464366/96‑1 (MQ=34) gTT‑AGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCCTCTGCGCACGACTGCCGGATGCGGCGTGa > 2:400830/1‑149 (MQ=37) aTCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGAt < 3:104863/76‑1 (MQ=34) aTCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGAt > 4:104863/1‑76 (MQ=34) cTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGcc < 3:236298/134‑1 (MQ=37) cTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGcc > 4:236298/1‑134 (MQ=37) aCGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGgcg > 2:235554/1‑149 (MQ=40) ggATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAAttt < 1:235554/149‑1 (MQ=255) ggATGCGGCGTAAACGCCTTATCCGGCCTAC‑‑‑‑GGCTCGG‑‑‑TTTGTAGGCCTGATAAGACGCGCTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGcc < 4:751128/122‑1 (MQ=11) ggATGCGGCGTAAACGCCTTATCCGGCCTAC‑‑‑‑GGCTCGG‑‑‑TTTGTAGGCCTGATAAGACGCGCTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGcc > 3:751128/1‑122 (MQ=11) ggATGCGGCGTAAACGCCTTATCCGGCCTAC‑‑‑‑GGCTCGG‑‑‑TTTGTAGGCCTGATAAGACGCGCTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGcc < 2:512048/122‑1 (MQ=11) ggATGCGGCGTAAACGCCTTATCCGGCCTAC‑‑‑‑GGCTCGG‑‑‑TTTGTAGGCCTGATAAGACGCGCTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGcc > 1:512048/1‑122 (MQ=11) ggcgTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGc > 3:399021/1‑149 (MQ=255) gcgTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGcc > 4:1105088/1‑149 (MQ=255) cgTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCt < 4:399021/149‑1 (MQ=255) tGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGa < 4:344327/149‑1 (MQ=255) | GTGCATTTTGTATGCCCATCCCAGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTT‑AGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGA > NC_000913/374899‑375192 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |