| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,823,581 | G→A | 5.2% | V63V (GTC→GTT) | recA ← | DNA recombination and repair protein; ssDNA‑dependent ATPase; synaptase; ssDNA and dsDNA binding protein; ATP‑dependent homologous DNA strand exchanger; recombinase A; LexA autocleavage cofactor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,823,581 | 0 | G | A | 5.2% | 2326.9 / 68.3 | 691 | V63V (GTC→GTT) | recA | DNA recombination and repair protein; ssDNA‑dependent ATPase; synaptase; ssDNA and dsDNA binding protein; ATP‑dependent homologous DNA strand exchanger; recombinase A; LexA autocleavage cofactor |
| Reads supporting (aligned to +/- strand): ref base G (340/315); new base A (18/18); total (358/333) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.65e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.26e-01 | |||||||||||
Only 100 of 734 total aligned reads displayed.
GATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAGGCGCATGATGGAGCCTTTACCAAATTGTTTCTCAATCTGGCCCAGTG > NC_000913/2823434‑2823729 | gATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTG‑CGCTGC‑GGCGATGACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTcga > 4:4759471/1‑149 (MQ=255) atatCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTcgac > 3:1326888/1‑149 (MQ=255) tCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCAACGAt > 2:2272643/1‑149 (MQ=255) gCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGc > 2:977745/1‑149 (MQ=255) gCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCAACGATACGGc < 3:2319071/149‑1 (MQ=255) cccAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGcc > 1:469380/1‑149 (MQ=255) ccAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGccc > 4:3898929/1‑149 (MQ=255) ccAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGccc > 1:6619522/1‑149 (MQ=255) cAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCa > 3:4113045/1‑149 (MQ=255) cAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCa > 1:2914428/1‑149 (MQ=255) gTTTACGTGCGTAGATTGGGTCCAGCGCGTGCTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATc > 2:2785851/1‑149 (MQ=255) aCGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCAACGATACGGCCCATCGGCa < 2:4579596/149‑1 (MQ=255) ttGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGcc > 1:1084345/1‑149 (MQ=255) gTCCAGCGCGGGTTCAACATCGATAAACGCACAGGTTTTACCTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCaa < 3:6903410/149‑1 (MQ=255) aGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAgcgc < 3:1150621/149‑1 (MQ=255) gcgTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGat < 3:4194944/149‑1 (MQ=255) tgtTCAGCATCGATAAACGCACAGGTTTTACCTTCAGGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATc > 4:6741883/1‑149 (MQ=255) gtTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAgcgc > 2:6957180/1‑142 (MQ=255) gtTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATcc > 3:2653775/1‑149 (MQ=255) ttCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCa > 2:5912934/1‑149 (MQ=255) ttCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCa < 4:5923608/149‑1 (MQ=255) tCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAg < 4:7071642/149‑1 (MQ=255) tCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAg < 2:6789179/149‑1 (MQ=255) cAGCATCGATAAACGCACAGGTTTTACCTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGt > 3:6273814/1‑149 (MQ=255) cAGCATCGATAAACGCACAGGTTTTACCTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGt < 4:3579097/149‑1 (MQ=255) gCATCGATAAACGCACAGGTTTTACCTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGATGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGa > 2:518524/1‑149 (MQ=255) gCATCGATAAACGCACAGGTTTTACCTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGa > 1:1561691/1‑149 (MQ=255) aTCGATAAACGCACAGGTTTTACCTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGcccc < 4:6104463/129‑1 (MQ=255) aTCGATAAACGCACAGGTTTTACCTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGaaa < 2:1329208/149‑1 (MQ=255) aTCGATAAACGCACAGGTTTTACCTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGaaa > 3:3689582/1‑149 (MQ=255) aTCGATAAACGCACAGGTTTTACCTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCAACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGaaa < 1:430168/149‑1 (MQ=255) gATAAACGCACAGGTTTTACCTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCg > 3:3240877/1‑149 (MQ=255) aTAAACGCACAGGTTTTACCTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGa > 3:6090570/1‑149 (MQ=255) tAAACGCACAGGTTTTACCTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGaa < 1:2076382/149‑1 (MQ=255) aatcGCTCAGGTTTTACCTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCCTACATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAAc < 2:4165751/146‑1 (MQ=255) aaCGCACAGGTTTTACCTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAAcc > 2:5726785/1‑149 (MQ=255) cacaGGTTTTACCTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTa < 1:6872928/149‑1 (MQ=255) acaGGTTTTACCTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTag > 3:6509740/1‑149 (MQ=255) gTTTTACCTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGAt < 3:814122/149‑1 (MQ=255) gTTTTACCTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGAt < 4:3199108/149‑1 (MQ=255) ttttACCTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATg > 1:3264266/1‑149 (MQ=255) ttACCTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGt < 1:4956501/149‑1 (MQ=255) tACCTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGtt < 1:7172292/149‑1 (MQ=255) tACCTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGtt < 1:236994/149‑1 (MQ=255) ctacACGCTG‑CGCTGC‑GGAGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCACGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTcc > 4:557684/4‑149 (MQ=255) cTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGAt > 2:3732397/1‑79 (MQ=255) cTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTcc > 4:6893883/1‑149 (MQ=255) cTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTcc < 2:2405103/149‑1 (MQ=255) tCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCcac > 1:3948766/1‑149 (MQ=255) tCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCcac < 1:6049319/149‑1 (MQ=255) ccACGCTGACGCTGCAGGTGATCGCC‑GCAGCG‑CAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCcac > 3:170285/2‑149 (MQ=38) cACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTcc > 3:547683/1‑146 (MQ=255) cACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCcaca < 1:3835172/149‑1 (MQ=255) cACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCcaca > 4:1435944/1‑149 (MQ=255) gctg‑cgctgc‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCCTCGGCAGACCACCTGTCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATcc < 2:6164899/149‑1 (MQ=255) gctg‑cgctgc‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATcc > 1:6321351/1‑149 (MQ=255) gctg‑cgctgc‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAAACGGTAGAGATGGTTTCCACATcc > 1:3595492/1‑149 (MQ=255) ctgc‑gGCGGTCACCTGCAGCGGCAGGGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGaa < 2:2116767/149‑1 (MQ=255) tgc‑gGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAAc < 2:7193601/149‑1 (MQ=255) ggcgATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGt > 3:3421541/1‑149 (MQ=255) gATCACCTGCAGCGTCAGCGTGGTTTTACCGAAAGATTCCGGTCCGTAGATTTCGACGATAAGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCtt < 2:484690/149‑1 (MQ=255) tCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCa < 2:1220221/149‑1 (MQ=255) cACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCAc < 4:5132158/149‑1 (MQ=255) aCCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCAcc > 3:5022751/1‑149 (MQ=255) aCCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCAcc < 2:1668024/149‑1 (MQ=255) cTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCa > 1:788962/1‑149 (MQ=255) tGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAg < 3:3904090/149‑1 (MQ=255) tGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCACATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAg < 1:1301331/149‑1 (MQ=255) tGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGGCCGTAGATTTCAACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAg > 2:6564774/1‑149 (MQ=255) tcagcgtGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAGGCGCatg > 4:2155452/1‑149 (MQ=255) cagcgtGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAGGCGCatga < 4:2396111/149‑1 (MQ=255) cagcgtGGTTGTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCAGCCCCAGGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAGGCCCatga > 4:4633762/1‑149 (MQ=255) gcgtGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAGGCGCatgatg < 4:3689582/149‑1 (MQ=255) gtGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAGGCGCATGATGGa < 4:3326674/149‑1 (MQ=255) gtGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAGGCGCATGATGGa < 4:2927912/149‑1 (MQ=255) tGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAGGCGCATGATGGAg < 4:6424681/149‑1 (MQ=255) gTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCACACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAGGCGCATGATGGAGcc < 3:4682501/149‑1 (MQ=255) tttACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAGGCGCATGATGGAGCCtt < 4:5381412/149‑1 (MQ=255) tACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAGGCGCATGATGGAGCCTTTa < 4:1138722/149‑1 (MQ=255) aCCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAGGCGCATGATGGAGCCTTTAc > 2:2977383/1‑149 (MQ=255) cGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCCGGCGCATGATGGAGCCTTTACCa > 4:1606813/1‑149 (MQ=255) cGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAGGCGCATGATGGAGCCTTTACCa > 3:5895080/1‑149 (MQ=255) cGGAAGATTCCGGTCCGTAGATTTCAACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAGGCGCATGATGGAGCCTTTACCa > 1:5579465/1‑149 (MQ=255) gAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTa < 4:1960001/87‑1 (MQ=255) gAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTcc < 3:922325/99‑1 (MQ=255) gAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAGGCGCATGATGGAGCCTTTACCaaa > 3:2136657/1‑149 (MQ=255) gAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAGGCGCATGATGGAGCCTTTACCaaa > 1:907138/1‑149 (MQ=255) aaGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAGGCGCATGATGGAGCCTTTACCAAAt < 4:6267466/149‑1 (MQ=255) aGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAGGCGCATGATGGAGCCTTTACCAAAtt > 4:4215524/1‑149 (MQ=255) tCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAGGCGCATGATGGAGCCTTTACCAAATTGttt < 2:3350424/149‑1 (MQ=255) tCCGGTCCGTAGATTTCAACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAGGCGCATGATGGAGCCTTTACCAAATTGttt < 3:2954919/149‑1 (MQ=255) ccGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAGGCGCATGATGGAGCCTTTACCAAATTGTTtc < 3:679146/149‑1 (MQ=255) cGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAg < 1:2626130/117‑1 (MQ=255) cGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAGGCGCATGATGGAGCCTTTACCAAATTGTTtct > 2:3932179/1‑149 (MQ=255) ccGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAGGCGCATGATGGAGCCTTTACCAAATTGTTTCTCAAt < 1:5912934/149‑1 (MQ=255) aGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAGGCGCATGATGGAGCCt > 2:5523062/1‑125 (MQ=255) aGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAGGCGCATGATGGAGCCTTTACCAAATTGTTTCTCAATCTgg > 4:765859/1‑149 (MQ=255) gATTTCAACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGACAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAGGCGCATGATGGAGCCTTTACCAAATTGTTTCTCAATCTGGc > 3:5350026/1‑149 (MQ=255) gacgaTACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAGGCGCATGATGGAGCCTTTACCAAATTGTTTCTCAATCTGGCCCAGtg > 1:5160099/1‑149 (MQ=255) gacgaTACGGCCCAGCAGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAACGCGCACCGGTAGAGATGCTTTCCACATCCATGGAACGGTCTTCAACCAGGCGCATGATCGACCCTTTGCCAAAGTGTTTCTCAATCTGGCCCAGtg > 4:2572836/1‑149 (MQ=255) | GATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTG‑CGCTGC‑GGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAGGCGCATGATGGAGCCTTTACCAAATTGTTTCTCAATCTGGCCCAGTG > NC_000913/2823434‑2823729 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |