Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 454,897 C→T 5.8% intergenic (+108/‑236) bolA → / → tig stationary‑phase morphogene, transcriptional repressor for mreB; also regulator for dacA, dacC, and ampC/peptidyl‑prolyl cis/trans isomerase (trigger factor)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913454,8970CT 5.8% 2536.4 / 84.4 760intergenic (+108/‑236)bolA/tigstationary‑phase morphogene, transcriptional repressor for mreB; also regulator for dacA, dacC, and ampC/peptidyl‑prolyl cis/trans isomerase (trigger factor)
Reads supporting (aligned to +/- strand):  ref base C (372/343);  new base T (24/20);  total (396/364)
Fisher's exact test for biased strand distribution p-value = 7.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 768 total aligned reads displayed.

TGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTG‑AAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGTAACAGGGAATGTGATTGATT  >  NC_000913/454750‑455045
                                                                                                                                                   |                                                                                                                                                     
  ccTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCAtt                                                                                                                                                    >  3:3045983/1‑149 (MQ=255)
   ctctCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTc                                                                                                                                                   >  4:5930320/1‑149 (MQ=255)
   ctctCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTc                                                                                                                                                   >  2:2503864/1‑149 (MQ=255)
    tctcCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCa                                                                                                                                                  >  2:1369668/1‑149 (MQ=255)
      tcctccCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGTATTCAGa                                                                                                                                                >  4:135115/1‑149 (MQ=255)
      tcctccCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGa                                                                                                                                                <  3:507237/149‑1 (MQ=255)
        ctccCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACg                                                                                                                                              >  2:6487297/1‑149 (MQ=255)
        ctccCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACg                                                                                                                                              >  4:5185630/1‑149 (MQ=255)
           ccTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTc                                                                                                                                                   <  2:505891/141‑1 (MQ=255)
               tCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGAGCTTTTTGCCCAGCATTCAGACGAAAATTg                                                                                                                                       >  3:4261542/1‑149 (MQ=255)
                    gAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCgg                                                                                                                                  <  3:1243037/149‑1 (MQ=255)
                       cAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGaa                                                                                                                               <  2:1016659/149‑1 (MQ=255)
                         ggAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAAtt                                                                                                                             <  1:1747344/149‑1 (MQ=255)
                         ggAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAAtt                                                                                                                             <  4:5457762/149‑1 (MQ=255)
                          gAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATtg                                                                                                                            >  3:1996102/1‑149 (MQ=255)
                           aaGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATtgt                                                                                                                           <  1:135201/149‑1 (MQ=255)
                           aaGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATtgt                                                                                                                           <  4:221189/149‑1 (MQ=255)
                            aGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATtgtg                                                                                                                          <  2:7051731/149‑1 (MQ=255)
                             tatgtgcgTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTG‑a                                                                                                                        <  4:6566683/144‑1 (MQ=255)
                                tCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTG‑aaaa                                                                                                                     >  1:2902764/1‑149 (MQ=255)
                                  gcgTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTG‑aaaaaa                                                                                                                   <  1:4904368/149‑1 (MQ=255)
                                  gcgTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTG‑aaaaaa                                                                                                                   <  3:3410052/149‑1 (MQ=255)
                                     tAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTG‑AAAAAATAc                                                                                                                <  4:1707287/149‑1 (MQ=255)
                                          aCGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCa                                                                                                                                                  <  4:3222701/111‑1 (MQ=255)
                                            gCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTG‑AAAAAATACGCGACAg                                                                                                         >  2:184173/1‑149 (MQ=255)
                                             cATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTG‑AAAAAATACGCGACAgc                                                                                                        <  2:2175634/149‑1 (MQ=255)
                                              aTTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTG‑AAAAAATACGCGACAgcg                                                                                                       >  2:80691/1‑149 (MQ=255)
                                              aTTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTG‑AAAAAATACGCGACAgcg                                                                                                       >  1:5320167/1‑149 (MQ=255)
                                               tttGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTG‑AAAAAATACGCGACAgcgc                                                                                                      >  1:2751427/1‑149 (MQ=255)
                                                  gCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTG‑AAAAAATACGCGACAGCGCGCa                                                                                                   >  3:3230121/1‑149 (MQ=255)
                                                    aaCTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTG‑AAAAAATACGCGACAGCGCGCAAt                                                                                                 >  3:916083/1‑149 (MQ=255)
                                                      cTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTG‑AAAAAATACGCGACAGCGCGCAATaa                                                                                               <  3:2592590/149‑1 (MQ=255)
                                                      cTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTG‑AAAAAATACGCGACAGCGCGCAATaa                                                                                               >  1:5140448/1‑149 (MQ=255)
                                                            gcgcTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTG‑AAAAAATACGCGACAGCGCGCAATAACCGTtc                                                                                         >  4:4071964/1‑149 (MQ=255)
                                                            gcgcTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTG‑AAAAAATACGCGACAGCGCGCAATAACCGTtc                                                                                         >  3:141873/1‑149 (MQ=255)
                                                              gctatcccAGTATGTTGCTAAAGATTTCATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTG‑AAAAAATACGCGACAGCGCGCAATAACCGTtctc                                                                                       <  4:6156597/143‑1 (MQ=255)
                                                              gcTTTTCCAGTATGTTGCTAAAGATCTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTG‑AAAAAATACGCGACAGCGCGCAATAACCGTtctc                                                                                       >  2:633349/1‑149 (MQ=255)
                                                                ttttCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTG‑AAAAAATACGCGACAGCGCGCAATAACCGTTCTCGa                                                                                     >  4:2543612/1‑149 (MQ=255)
                                                                ttttCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTG‑AAAAAATACGCGACAGCGCGCAATAACCGTTCTCGa                                                                                     >  4:1902673/1‑149 (MQ=255)
                                                                 tttCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTG‑AAAAAATACGCGACAGCGCGCAATAACCGTTCTCGAc                                                                                    <  1:7091436/149‑1 (MQ=255)
                                                                 tttCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTG‑AAAAAATACGCGACAGCGCGCAATAACCGTTCTCGAc                                                                                    <  3:6349120/149‑1 (MQ=255)
                                                                     cAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTG‑AAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCAt                                                                                <  1:5660345/149‑1 (MQ=255)
                                                                        tATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTG‑AAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATaaa                                                                             >  4:3456380/1‑149 (MQ=255)
                                                                         aTGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTG‑AAAAAATACGCGACAGCGCGCAATAACCGTTCTCTACTCATaaaa                                                                            <  1:5870318/149‑1 (MQ=255)
                                                                         aTGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTG‑AAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATaaaa                                                                            <  1:3479978/149‑1 (MQ=255)
                                                                         aTGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTG‑AAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATaaaa                                                                            >  3:277848/1‑149 (MQ=255)
                                                                          tGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTG‑AAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAg                                                                           <  4:816005/149‑1 (MQ=255)
                                                                           gTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTG‑AAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGt                                                                          >  4:4445010/1‑149 (MQ=255)
                                                                           gTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTG‑AAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGt                                                                          >  2:6735309/1‑149 (MQ=255)
                                                                            ttGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCACGGGAATTGTG‑AAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTg                                                                         >  2:405828/1‑149 (MQ=255)
                                                                             tGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTG‑AAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGa                                                                        <  2:5195194/149‑1 (MQ=255)
                                                                             tGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTG‑AAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGa                                                                        <  2:3725534/149‑1 (MQ=255)
                                                                                 aaaGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTG‑AAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGcc                                                                    >  2:976633/1‑149 (MQ=255)
                                                                                 aaaGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTG‑AAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGcc                                                                    >  2:2766489/1‑149 (MQ=255)
                                                                                   aGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTG‑AAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGc                                                                  <  1:4752525/149‑1 (MQ=255)
                                                                                        ttATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTG‑AAAAAATACACGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATaa                                                             >  1:4082777/1‑149 (MQ=255)
                                                                                         tATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTG‑AAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAAt                                                            >  4:6839559/1‑149 (MQ=255)
                                                                                         tATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTG‑AAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAAt                                                            <  1:1035598/149‑1 (MQ=255)
                                                                                           aacaaaaCGGCCCGCAGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTG‑AAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGc                                                          >  3:4863987/4‑149 (MQ=255)
                                                                                            gAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTG‑AAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGcc                                                         >  1:6941458/1‑149 (MQ=255)
                                                                                            gAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTG‑AAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGcc                                                         <  2:1558704/149‑1 (MQ=255)
                                                                                            acaaaaCGGCCCGCAGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTG‑AAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGcc                                                         <  2:4749996/147‑1 (MQ=255)
                                                                                             aaaaaCGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCCGACGAAAATTGCCCGGGAATTGTG‑AAAAAATACGCGACCGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATCATGCcg                                                        >  3:5263432/1‑149 (MQ=255)
                                                                                             aaaaaCGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTG‑AAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCcg                                                        >  1:2422265/1‑149 (MQ=255)
                                                                                             aaaaaCGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTG‑AAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCcg                                                        >  1:2109424/1‑149 (MQ=255)
                                                                                              aaaaCGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTG‑AAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCcgc                                                       <  3:6898023/149‑1 (MQ=255)
                                                                                              aaaaCGGCCCGCAGGCCGTTTTGTTTTGTCTGGGTTTCGCGCTTTTTGCCCAGTATTCAGACGAAAATTGCCCGGGAAATGTG‑AAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCcgc                                                       >  4:3785117/1‑149 (MQ=255)
                                                                                                    gCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATGGTG‑AAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTa                                                 >  2:2991068/1‑149 (MQ=255)
                                                                                                        gCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTAttt                                              <  2:3362627/149‑1 (MQ=255)
                                                                                                        gCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTG‑AAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTg                                             >  1:422316/1‑149 (MQ=255)
                                                                                                          gggCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTG‑AAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCTTCCTATTTGaa                                           <  1:3807227/149‑1 (MQ=255)
                                                                                                          gggCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTG‑AAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGaa                                           <  1:313985/149‑1 (MQ=255)
                                                                                                          gggCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTG‑AAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGaa                                           <  4:56498/149‑1 (MQ=255)
                                                                                                             ccGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTG‑AAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGTCGCGTCCTATTTGAATGc                                        >  3:1750817/1‑149 (MQ=255)
                                                                                                               gttttgttttGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTG‑AAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCtt                                      >  1:1932243/1‑149 (MQ=255)
                                                                                                               gttttgttttGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTG‑AAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCtt                                      >  4:3364483/1‑149 (MQ=255)
                                                                                                                ttttgttttgTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTG‑AAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCttt                                     <  1:80286/149‑1 (MQ=255)
                                                                                                                ttttgttttgTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTG‑AAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCttt                                     >  2:3527149/1‑149 (MQ=255)
                                                                                                                  ttgttttgtCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGACAATTCCCCGGACATTCTT‑AAACAACACGCCACACCGCCCAACAACCTTTCTCCACCCACAAAAATGATGCCGATATAATGCCGCGTCATATTTTAATGCctttg                                   <  2:4306932/149‑3 (MQ=255)
                                                                                                                     ttttgtCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTG‑AAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCggg                                 >  2:6098846/1‑148 (MQ=255)
                                                                                                                          tCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTG‑AAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGAtt                           <  3:4445010/149‑1 (MQ=255)
                                                                                                                           cTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTG‑AAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTc                          >  2:555043/1‑149 (MQ=255)
                                                                                                                            tGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTG‑AAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCt                         <  1:5503006/149‑1 (MQ=255)
                                                                                                                            tGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTG‑AAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCt                         >  1:4442023/1‑149 (MQ=255)
                                                                                                                             ggATTTTGCGCTTTTTGCCCAGTATTCAGACGAAAATTGCCCGGGAATTGTG‑AAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTg                        <  4:4794839/149‑1 (MQ=255)
                                                                                                                             ggATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTG‑AAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTg                        <  1:6518467/149‑1 (MQ=255)
                                                                                                                                    gcgcTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTG‑AAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGTAACAg                 >  1:6643022/1‑149 (MQ=255)
                                                                                                                                      gcTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTG‑AAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGTAACAggg               >  1:6161068/1‑149 (MQ=255)
                                                                                                                                      gcTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTG‑AAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGTAACAggg               >  4:2919224/1‑149 (MQ=255)
                                                                                                                                      gcTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTG‑AAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGTAACAggg               >  3:4631963/1‑149 (MQ=255)
                                                                                                                                       cTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTG‑AAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGTAACAGGGa              >  3:4164781/1‑149 (MQ=255)
                                                                                                                                        tttttGCCCAGTATTCAGACGAAAATTGCCCGGGAATTGTG‑AAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGTAACAGGGaa             >  2:5724986/1‑149 (MQ=255)
                                                                                                                                           ttGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTG‑AAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGTAACAGGGAAtgt          <  3:2319774/149‑1 (MQ=255)
                                                                                                                                             gCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTG‑AAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGTAACAGGGAATGtga        <  3:5562083/149‑1 (MQ=255)
                                                                                                                                              cccAGTATTCAGACGAAAATTGCCCGGGAATTGTG‑AAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGTAACAGGGAATGtgat       <  2:204631/149‑1 (MQ=255)
                                                                                                                                               ccAGCATTCAGACGAAAATTGCCCGGGAATTGTG‑AAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGTAACAGGGAATGtgatt      <  3:3724198/149‑1 (MQ=255)
                                                                                                                                               ccAGCATTCAGACGAAAATTGCCCGGGAATTGTG‑AAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGTAACAGGGAATGtgatt      >  1:153839/1‑149 (MQ=255)
                                                                                                                                               ccAGCATTCAGACGAAAATTGCCCGGGAATTGTG‑AAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGTAACACGGAATGtgatt      <  3:6165502/149‑1 (MQ=255)
                                                                                                                                                 aGCATTCAGACGAAAATTGCCCGGGAATTGTG‑AAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGTAACAGGGAATGtgattga    <  4:4631963/149‑1 (MQ=255)
                                                                                                                                                 aGCATTCAGACGAAAATTGCCCGGGAATTGTG‑AAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGTAACAGGGAATGtgattga    <  3:6016303/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
TGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTG‑AAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGTAACAGGGAATGTGATTGATT  >  NC_000913/454750‑455045

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑