| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,888,212 | Δ1 bp | 14.0% | intergenic (+20/‑223) | mnmE → / → tnaC | tRNA U34 5‑methylaminomethyl‑2‑thiouridine modification GTPase/tryptophanase leader peptide |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,888,212 | 0 | T | . | 14.0% | 136.0 / 17.3 | 43 | intergenic (+20/‑223) | mnmE/tnaC | tRNA U34 5‑methylaminomethyl‑2‑thiouridine modification GTPase/tryptophanase leader peptide |
| Reads supporting (aligned to +/- strand): ref base T (18/19); new base . (3/3); total (21/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01 | |||||||||||
GTGAACTGCTGGCGGAAGAGTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAA > NC_000913/3888074‑3888279 | gTGAACTGCTGGCGGAAGAGTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTc < 4:796204/149‑1 (MQ=255) gCGGAAGAGTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGgctt > 4:69537/1‑149 (MQ=255) ggAAGAGTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCA‑TCTGACAGTCAG‑aatgc > 3:848954/1‑138 (MQ=255) ggAAGAGTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCA‑TCTGACAGTCAG‑aatgc < 4:848954/142‑5 (MQ=255) cacaGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATTGTTACCACTc > 1:722637/1‑149 (MQ=255) cacaGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATTGTTACCACTc < 3:69537/149‑1 (MQ=255) acaGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGa > 3:744798/1‑113 (MQ=255) acaGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGa < 4:744798/113‑1 (MQ=255) cagAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATTGTTa > 3:1446659/1‑138 (MQ=255) cagAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATTGTTa < 4:1446659/138‑1 (MQ=255) ttAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATTGTTACCa < 2:841982/135‑1 (MQ=255) ttAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATTGTTACCa > 1:841982/1‑135 (MQ=255) tCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCA‑TCTGACAGTCAG‑aatgc > 1:213328/1‑100 (MQ=39) tCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCA‑TCTGACAGTCAG‑aatgc < 2:213328/104‑5 (MQ=39) cGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTa < 2:722637/149‑1 (MQ=255) gggAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTaaa < 2:454465/149‑1 (MQ=255) gggAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTaaa > 1:454465/1‑149 (MQ=255) ggAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGAc > 4:494274/1‑87 (MQ=255) ggAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGAc < 3:494274/87‑1 (MQ=255) tttACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATTGTTACCACTCCTGTTCTTCCTCAACCCtttt < 4:1403623/138‑1 (MQ=255) tttACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCtttt > 3:1403623/1‑138 (MQ=255) tttACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCtttt < 4:372768/138‑1 (MQ=255) tttACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCtttt > 3:372768/1‑138 (MQ=255) ttCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATTGTTACCACTCCTGTTAt < 1:529377/119‑1 (MQ=255) ttCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATTGTTACCACTCCTGTTAt > 2:529377/1‑119 (MQ=255) gacgacCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCAt < 3:1296479/97‑1 (MQ=255) gacgacCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCAt > 4:1296479/1‑97 (MQ=255) acgacCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCa > 4:584475/1‑78 (MQ=255) acgacCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCa < 3:584475/78‑1 (MQ=255) ctcCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCttttt > 3:416433/1‑107 (MQ=255) ctcCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCttttt < 4:416433/107‑1 (MQ=255) tcCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATTGTTAc > 4:942156/1‑79 (MQ=255) tcCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATTGTTAc < 3:942156/79‑1 (MQ=255) tcCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTcc < 4:406341/94‑1 (MQ=255) tcCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTcc > 3:406341/1‑94 (MQ=255) cAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGgcttcgc > 3:757567/1‑66 (MQ=255) cAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGgcttcgc < 4:757567/66‑1 (MQ=255) cAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATTGTTACCACTCCTGtt < 2:33115/87‑1 (MQ=255) cAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATTGTTACCACTCCTGtt > 1:33115/1‑87 (MQ=255) aagtaaCCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCttt < 4:1302850/86‑1 (MQ=255) aagtaaCCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCttt > 3:1302850/1‑86 (MQ=255) aGCCGCA‑TCTGACAGTCAGAATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCtttt > 4:1345243/1‑69 (MQ=21) aGCCGCA‑TCTGACAGTCAGAATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCtttt < 3:1345243/69‑1 (MQ=21) | GTGAACTGCTGGCGGAAGAGTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAA > NC_000913/3888074‑3888279 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |