Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,285,866	Δ1 bp	6.3%	coding (1506/1959 nt)	acs ←	acetyl‑CoA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,285,866	0	C	.	6.3%	341.9 / 16.0	95	coding (1506/1959 nt)	acs	acetyl‑CoA synthetase
Reads supporting (aligned to +/- strand):  ref base C (45/44);  new base . (2/4);  total (47/48)
Fisher's exact test for biased strand distribution p-value = 6.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.42e-01

CTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCC  >  NC_000913/4285730‑4286001                                                                                                                                         |                                                                                                                                         ctacGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACG‑GCGCGCGTcgcga                                                                                                                             <  1:817861/149‑3 (MQ=255) ctacGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACG‑GCGCGCGTcgcga                                                                                                                             <  4:248447/149‑3 (MQ=255)               gCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATAtt                                                                                                               >  3:438082/1‑149 (MQ=255)                 aaTCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATAtttt                                                                                                             >  4:599390/1‑149 (MQ=255)                 aaTCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATAtttt                                                                                                             <  2:506317/149‑1 (MQ=255)                     ttCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATAc                                                                                                                    <  3:302019/138‑1 (MQ=255)                     ttCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATAc                                                                                                                    >  4:302019/1‑138 (MQ=255)                     ttCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGaa                                                                                                         >  4:736531/1‑149 (MQ=255)                        ggATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATAttt                                                                                                              >  2:1310207/1‑141 (MQ=255)                        ggATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATAttt                                                                                                              <  1:1310207/141‑1 (MQ=255)                            gcgcCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGgag                                                                                                  <  4:438082/149‑1 (MQ=255)                              gcCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGaa                                                                                                >  2:937740/1‑149 (MQ=255)                                       gcCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTg                                                                                       >  2:525862/1‑149 (MQ=255)                                               aaTCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAAc                                                                               >  2:545744/1‑149 (MQ=255)                                               aaTCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAAc                                                                               >  4:1455841/1‑149 (MQ=255)                                                aTCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGaaa                                                                                                                       >  1:1312513/1‑108 (MQ=255)                                                aTCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGaaa                                                                                                                       <  2:1312513/108‑1 (MQ=255)                                                aTCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACg                                                                              >  3:1041044/1‑149 (MQ=255)                                                  ctctGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGtt                                                                            <  1:545744/149‑1 (MQ=255)                                                   tctGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTc                                                                           <  1:937740/149‑1 (MQ=255)                                                   tctGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTc                                                                           <  1:525862/149‑1 (MQ=255)                                                   tctGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTc                                                                           <  3:599390/149‑1 (MQ=255)                                                   tctGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTc                                                                           >  3:702491/1‑149 (MQ=255)                                                   tctGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTc                                                                           <  3:1455841/149‑1 (MQ=255)                                                     tGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATAc                                                                                                                    <  1:366045/106‑1 (MQ=255)                                                     tGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATAc                                                                                                                    >  2:366045/1‑106 (MQ=255)                                                     tGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGt                                                                         <  3:736531/149‑1 (MQ=255)                                                       ccGTCCCCAGACGGTGACCGGAGACGTTCAGCCCGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACG‑GCGCGCGTcgcga                                                                                                                             >  3:417100/1‑93 (MQ=255)                                                       ccGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCTGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGGTCATAACGTTCGTGa                                                                       >  1:1122746/1‑149 (MQ=255)                                                       ccGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACG‑GCGCGCGTcgcga                                                                                                                             <  4:417100/95‑3 (MQ=255)                                                         gTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACG‑GCGCGCGTcgcgat                                                                                                                            >  2:1206719/1‑91 (MQ=255)                                                         gTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACG‑GCGCGCGTcgcgat                                                                                                                            <  1:1206719/94‑4 (MQ=255)                                                           ccccAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGc                                                                   <  4:1041044/149‑1 (MQ=255)                                                             ccAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTTAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGc                                                                   <  1:958812/147‑1 (MQ=255)                                                             ccAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGt                                                                                                      >  3:1064004/1‑112 (MQ=255)                                                             ccAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGt                                                                                                      <  4:1064004/112‑1 (MQ=255)                                                             ccAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGc                                                                   >  2:958812/1‑147 (MQ=255)                                                             ccAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCa                                                                 >  3:533888/1‑149 (MQ=255)                                                             ccAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCa                                                                 >  2:819310/1‑149 (MQ=255)                                                                   ggTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGc                                                           <  2:1122746/149‑1 (MQ=255)                                                                       aCCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTAc                                                       >  2:1180548/1‑149 (MQ=255)                                                                       aCCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTAc                                                       >  3:614943/1‑149 (MQ=255)                                                                          ggAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTAcgcg                                                    >  4:1220526/1‑149 (MQ=255)                                                                           gagaCGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACgcgc                                                   <  1:1180548/149‑1 (MQ=255)                                                                              aCGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATAttttt                                                                                                            <  2:494426/89‑1 (MQ=255)                                                                              aCGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATAttttt                                                                                                            >  1:494426/1‑89 (MQ=255)                                                                              aCGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTg                                                >  1:467895/1‑149 (MQ=255)                                                                                gTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGAc                                              >  2:175207/1‑149 (MQ=255)                                                                                 ttCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGtt                                                                                     <  4:291522/109‑1 (MQ=255)                                                                                 ttCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGtt                                                                                     >  3:291522/1‑109 (MQ=255)                                                                                 ttCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGAcc                                             >  2:1239275/1‑149 (MQ=255)                                                                                     gCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACgcgc                                                   <  4:175752/139‑1 (MQ=255)                                                                                     gCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACgcgc                                                   >  3:175752/1‑139 (MQ=255)                                                                                      cACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGcc                                        <  4:533888/149‑1 (MQ=255)                                                                                      cACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGcc                                        >  4:1431155/1‑149 (MQ=255)                                                                                      cACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGcc                                        >  2:337395/1‑149 (MQ=255)                                                                                       aCGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTc                                                                           >  2:1276399/1‑113 (MQ=255)                                                                                       aCGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTc                                                                           <  1:1276399/113‑1 (MQ=255)                                                                                       aCGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCa                                       <  4:702491/149‑1 (MQ=255)                                                                                          tcgtcCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTg                                                                                                     <  2:223140/84‑1 (MQ=255)                                                                                          tcgtcCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTg                                                                                                     >  1:223140/1‑84 (MQ=255)                                                                                             tcCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTc                                 <  1:819310/149‑1 (MQ=255)                                                                                               cacaCGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCgg                               <  3:1220526/149‑1 (MQ=255)                                                                                                 cacGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTg                             >  2:476974/1‑149 (MQ=255)                                                                                                  acGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGa                            <  4:614943/149‑1 (MQ=255)                                                                                                  acGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGa                            <  1:1239275/149‑1 (MQ=255)                                                                                                  acGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGa                            <  3:1431155/149‑1 (MQ=255)                                                                                                    gCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGAtt                          <  1:476974/149‑1 (MQ=255)                                                                                                     cccGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTa                         <  2:833458/149‑1 (MQ=255)                                                                                                      ccGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTc                                                                           >  4:984213/1‑98 (MQ=255)                                                                                                      ccGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTc                                                                           <  3:984213/98‑1 (MQ=255)                                                                                                         gTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTAcgc                                                     <  3:389064/117‑1 (MQ=255)                                                                                                         gTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTAcgc                                                     >  4:389064/1‑117 (MQ=255)                                                                                                              ccAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTAc                >  3:812954/1‑149 (MQ=255)                                                                                                                     tAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGt         <  4:812954/149‑1 (MQ=255)                                                                                                                     tAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGt         <  1:175207/149‑1 (MQ=255)                                                                                                                     tAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGt         <  1:337395/149‑1 (MQ=255)                                                                                                                      aGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTAcgcg                                                    >  4:1029204/1‑105 (MQ=255)                                                                                                                      aGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTAcgcg                                                    <  3:1029204/105‑1 (MQ=255)                                                                                                                         cATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGAtt                          <  4:1292667/128‑1 (MQ=255)                                                                                                                         cATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGAtt                          >  3:1292667/1‑128 (MQ=255)                                                                                                                           tCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGcccc   >  1:17110/1‑149 (MQ=255)                                                                                                                            cTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGccccc  >  3:486641/1‑149 (MQ=255)                                                                                                                               cATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAg                     <  4:634770/127‑1 (MQ=255)                                                                                                                               cATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAg                     >  3:634770/1‑127 (MQ=255)                                                                                                                                 tCGCGCCGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTAc                        <  2:56995/122‑1 (MQ=255)                                                                                                                                 tCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTc                                                                           >  2:995918/1‑71 (MQ=255)                                                                                                                                 tCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTc                                                                           <  1:995918/71‑1 (MQ=255)                                                                                                                                 tCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTAc                        >  1:56995/1‑122 (MQ=255)                                                                                                                                 tCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTg        >  4:855005/1‑138 (MQ=255)                                                                                                                                 tCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTg        <  3:855005/138‑1 (MQ=255)                                                                                                                                 tCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGcc     >  2:934662/1‑141 (MQ=255)                                                                                                                                 tCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGcc     <  1:934662/141‑1 (MQ=255)                                                                                                                                  cgcgACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGc                                         >  3:1403600/1‑104 (MQ=255)                                                                                                                                  cgcgACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGc                                         <  4:1403600/104‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         CTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCC  >  NC_000913/4285730‑4286001

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑