| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,596,878:1 | +G | 11.3% | intergenic (‑141/+27) | ypfJ ← / ← purC | putative neutral zinc metallopeptidase/phosphoribosylaminoimidazole‑succinocarboxamide synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,596,878 | 1 | . | G | 11.3% | 235.9 / 28.6 | 62 | intergenic (‑141/+27) | ypfJ/purC | putative neutral zinc metallopeptidase/phosphoribosylaminoimidazole‑succinocarboxamide synthetase |
| Reads supporting (aligned to +/- strand): ref base . (27/28); new base G (3/4); total (30/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.38e-01 | |||||||||||
GCATAGATACCTCAACAATATATTCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCTTTGTCCATTTTCTCCAGCGTTTCTTTGTCCCACAGGCGGC > NC_000913/2596734‑2597026 | gCATAGATACCTCAACAATATATTCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCAc < 2:353239/149‑1 (MQ=255) gATACCTCAACAATATATTCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTG‑CAGCTGGCACGGCaa < 2:1167957/149‑1 (MQ=255) aacaaTATATTCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCACGGCAAGAtgatga > 2:1295547/1‑149 (MQ=255) tatatTCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCACGGCAAGatgatg < 3:924267/143‑1 (MQ=255) tatatTCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCACGGCAAGatgatg > 4:924267/1‑143 (MQ=255) ttCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCAc < 2:662597/127‑1 (MQ=255) ttCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCAc > 1:662597/1‑127 (MQ=255) gAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCACGGCAAgatgat > 1:1406102/1‑129 (MQ=255) gAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCACGGCAAgatgat < 2:1406102/129‑1 (MQ=255) aTCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTG‑CAGCTGGCACGGc > 3:1183662/1‑115 (MQ=255) aTCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTG‑CAGCTGGCACGGc < 4:1183662/115‑1 (MQ=255) gTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCACGGCaa > 4:237974/1‑114 (MQ=255) gTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCACGGCaa < 3:237974/114‑1 (MQ=255) agGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCACGGCAAGATGATGaa < 4:149884/110‑1 (MQ=255) agGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCACGGCAAGATGATGaa > 3:149884/1‑110 (MQ=255) ggAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTG‑CAGCTGGCACGGCaa < 1:611971/100‑1 (MQ=255) ggAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTG‑CAGCTGGCACGGCaa > 2:611971/1‑100 (MQ=255) gAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCACGGCaa > 4:412459/1‑99 (MQ=255) gAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCACGGCaa < 3:412459/99‑1 (MQ=255) gAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCACGGCAAGATGATGAACAGAAAAATCa < 2:319611/119‑1 (MQ=255) gAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCACGGCAAGATGATGAACAGAAAAATCa > 1:319611/1‑119 (MQ=255) ttACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCAc < 3:466275/89‑1 (MQ=255) ttACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCAc < 1:1124384/89‑1 (MQ=255) ttACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCAc > 2:1124384/1‑89 (MQ=255) ttACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCAc > 4:466275/1‑89 (MQ=255) acaGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTa > 2:1459274/1‑120 (MQ=255) acaGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTa < 1:1459274/120‑1 (MQ=255) cGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATc < 1:1295547/149‑1 (MQ=255) gATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCACGGCAAGATGATGAACa < 2:1295213/88‑1 (MQ=255) gATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCACGGCAAGATGATGAACa > 1:1295213/1‑88 (MQ=255) gaggaATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGcggcgg > 1:918314/1‑110 (MQ=255) gaggaATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGcggcgg < 2:918314/110‑1 (MQ=255) gCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGaaa < 2:352208/149‑1 (MQ=255) gCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGaaa > 1:352208/1‑149 (MQ=255) ttCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACgg > 3:1141836/1‑149 (MQ=255) gCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAggcggc < 1:588155/90‑1 (MQ=255) gCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCACGGCAAGATGAGGAACAGAAAAATCAGTCCAGCTGTACACCCAggcggc > 2:588155/1‑90 (MQ=255) tatTCCGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCTTTGTCCAt < 4:1141836/149‑1 (MQ=255) ttCCGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCTTTGTACAttt > 3:988555/1‑149 (MQ=255) ggTTGTCTTGCCGTGCCAGCT‑GCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCTTTGTACATTTTCTCCAGCGt < 4:988555/149‑1 (MQ=255) ttGTCTTGCCGTGCCAGCT‑GCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATa > 3:325331/1‑87 (MQ=255) ttGTCTTGCCGTGCCAGCT‑GCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATa < 4:325331/87‑1 (MQ=255) tCTTGCCGTGCCAGCT‑GCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCTTTGTCCAt > 1:1326340/1‑132 (MQ=255) tCTTGCCGTGCCAGCT‑GCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCTTTGTCCAt < 2:1326340/132‑1 (MQ=255) ttGCCGTGCCAGCT‑GCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCTTTGTCCATTTTCTCCAGCGTTTCTTTg > 1:546707/1‑149 (MQ=255) tGCCGTGCCAGCT‑GCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCTTTGTCCATTTtctc > 3:303120/1‑135 (MQ=255) tGCCGTGCCAGCT‑GCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCTTTGTCCATTTtctc < 4:303120/135‑1 (MQ=255) tGCCGTG‑CAGCTGGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAgg < 2:59584/103‑1 (MQ=255) tGCCGTG‑CAGCTGGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAgg > 1:59584/1‑103 (MQ=255) gCCGTGCCAGCT‑GCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCt > 1:1175593/1‑120 (MQ=255) gCCGTGCCAGCT‑GCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCt < 2:1175593/120‑1 (MQ=255) ccGTGCCAGCT‑GCACGGCAAGATGATGAACAGAATAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCTTTGTCCATTTTCTCCAGCGTTTCTTTGTc < 4:953074/148‑1 (MQ=255) ccGTGCCAGCT‑GCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCTTTGTCCATTTTCTCCAGCGTTTCTTTGTc < 2:160241/148‑1 (MQ=255) ccGTGCCAGCT‑GCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCTTTGTCCATTTTCTCCAGCGTTTCTTTGTc > 3:953074/1‑148 (MQ=255) ccGTGCCAGCT‑GCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCTTTGTCCATTTTCTCCAGCGTTTCTTTGTc > 1:160241/1‑148 (MQ=255) ccAGCT‑GCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCTTTGTCCATTTTCTCCAGCGTTTCttt > 3:138746/1‑140 (MQ=255) ccAGCT‑GCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCTTTGTCCATTTTCTCCAGCGTTTCttt < 4:138746/140‑1 (MQ=255) aGCT‑GCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCa > 1:845476/1‑50 (MQ=255) aGCT‑GCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCa < 2:845476/50‑1 (MQ=255) aGCT‑GCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCTTTGTCCATTTTCTCCAGCGTTTCTTTGTCCCACAggc < 2:546707/149‑1 (MQ=255) gCT‑GCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCTTTGTCCATTTTCTCCAGCGTTTCTTTGt < 1:313648/139‑1 (MQ=255) gCT‑GCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCTTTGTCCATTTTCTCCAGCGTTTCTTTGt > 2:313648/1‑139 (MQ=255) t‑GCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCTTTGTCCATTTTCTCCAGCGTTTCTTTGTCCCACAggcggc > 1:808910/1‑149 (MQ=255) | GCATAGATACCTCAACAATATATTCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCTTTGTCCATTTTCTCCAGCGTTTCTTTGTCCCACAGGCGGC > NC_000913/2596734‑2597026 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |