Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	192,573:1	+G	7.7%	coding (719/726 nt)	pyrH →	uridylate kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	192,573	1	.	G	7.7%	277.8 / 19.3	65	coding (719/726 nt)	pyrH	uridylate kinase
Reads supporting (aligned to +/- strand):  ref base . (28/32);  new base G (3/2);  total (31/34)
Fisher's exact test for biased strand distribution p-value = 6.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.91e-01

GAAGTGCTGGAAAAAGAGCTGAAAGTCATGGACCTGGCGGCCTTCACGCTGGCTCGTGACCATAAATTACCGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTGCAGGCAGTTTGGTCACGGCCAGCGCGCAGCAACCGGAGCGTACAAAAGTACGTGAGGATG  >  NC_000913/192425‑192720                                                                                                                                                      |                                                                                                                                                    gAAGTGCTGGAAAAAGAGCTGAAAGTCATGGACCTGGCGGCCTTCACGCTGGCTCGTGACCATAAATTACCGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAc                                                                                                                                                      <  2:514507/149‑1 (MQ=255) gAAGTGCTGGAAAAAGAGCTGAAAGTCATGGACCTGGCGGCCTTCACGCTGGCTCGTGACCATAAATTACCGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAc                                                                                                                                                      <  4:330569/149‑1 (MQ=255)     tGCTGGAAAAAGAGCTGAAAGTCATGGACCTGGCGGCCTTCACGCTGGCTCGTGACCATAAATTACCGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGaa                                                                                                                                                 <  4:600752/149‑1 (MQ=255)          gAAAAAGAGCTGAAAGTCATGGACCTGGCGGCCTTCACGCTGGCTCGTGACCATAAATTACCGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGaat                                                                                                                                                <  1:460104/145‑1 (MQ=255)          gAAAAAGAGCTGAAAGTCATGGACCTGGCGGCCTTCACGCTGGCTCGTGACCATAAATTACCGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGaat                                                                                                                                                >  2:460104/1‑145 (MQ=255)               agagCTGAAAGTCATGGACCTGGCGGCCCTCACGCTGGCTCGTGACCATAAATTACCGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGt                                                                                                                                       >  3:613424/1‑149 (MQ=255)                 agCTGAAAGTCATGGACCTGGCGGCCTTCACGCTGGCTCGTGACCATAAATTACCGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGa                                                                                                                                     <  1:363063/149‑1 (MQ=255)                 agCTGAAAGTCATGGACCTGGCGGCCTTCACGCTGGCTCGTGACCATAAATTACCGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGCGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGa                                                                                                                                     <  2:383364/149‑1 (MQ=255)                  gCTGAAAGTCATGGACCTGGCGGCCTTCACGCTGGCTCGTGACCATAAATTACCGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGAt                                                                                                                                    <  1:424731/149‑1 (MQ=255)                          tCATGGACCTGGCGGCCTTCACGCTGGCTCGTGACCATAAATTACCGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGa                                                                                                                                     >  4:630649/1‑140 (MQ=255)                          tCATGGACCTGGCGGCCTTCACGCTGGCTCGTGACCATAAATTACCGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGa                                                                                                                                     <  3:630649/140‑1 (MQ=255)                            aTGGACCTGGCGGCCTTCACGCTGGCTCGTGACCATAAATTACCGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGataaataa                                                                                                                          <  1:453935/149‑1 (MQ=255)                            aTGGACCTGGCGGCCTTCACGCTGGCTCGTGACCATAAATTACCGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGataaataa                                                                                                                          >  1:85693/1‑149 (MQ=255)                            aTGGACCTGGCGGCCTTCACGCTGGCTCGTGACCATAAATTACCGATTCGTGTTTTCAATATCAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGataaataa                                                                                                                          <  3:214586/149‑1 (MQ=255)                             tGGACCTGGCGGCCTTCACGCTGGCTCGTGACCATAAATTACCGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAg                                                                                                                         <  3:514855/149‑1 (MQ=255)                             tGGACCTGGCGGCCTTCACGCTGGCTCGTGACCATAAATTACCGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAg                                                                                                                         <  4:240496/149‑1 (MQ=255)                              ggACCTGGCGGCCTTCACGCTGGCTCGTGACCATAAATTACCGTTTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGataaataa                                                                                                                          <  3:83183/147‑1 (MQ=255)                              ggACCTGGCGGCCTTCACGCTGGCTCGTGACCATAAATTACCGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAgg                                                                                                                        <  4:10884/149‑1 (MQ=255)                              ggACCTGGCGGCCTTCACGCTGGCTCGTGACCATAAATTACCGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTCTAATCAC‑GGAATAATTCCCGTGATGGataaataa                                                                                                                          >  4:83183/1‑147 (MQ=255)                              ggACCTGGCGGCCCTCACGCTGGCTCGTGACCATAAATTACCGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAgg                                                                                                                        <  4:613424/149‑1 (MQ=255)                                  cTGGCGGCCTTCACGCTGGCTCGTGACCATAAATTACCGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTaa                                                                                                                    <  4:131151/149‑1 (MQ=255)                                    ggcggcCTTCACGCTGGCTCGTGACCATAAATTACCGATTCGTGTTTTCAATATGAACTAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCTGTGTTGGATAAGTAAGGGTAAGa                                                                                                                  <  2:531452/149‑1 (MQ=255)                                        gcCTTCACGCTGGCTCGTGACCATAAATTACCGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAGAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTcc                                                                                                              <  2:530214/149‑1 (MQ=255)                                                     cTCGTGACCATAAATTACCGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGt                                                                                                                                       >  1:672885/1‑111 (MQ=255)                                                     cTCGTGACCATAAATTACCGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGt                                                                                                                                       <  2:672885/111‑1 (MQ=255)                                                      tCGTGACCATAAATTACCGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATcgcg                                                                                                <  3:679798/149‑1 (MQ=255)                                                      tCGTGACCATAAATTACCGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATcgcg                                                                                                >  2:309263/1‑149 (MQ=255)                                                             cATAAATTACCGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCgggg                                                                                             >  2:426250/1‑145 (MQ=255)                                                             cATAAATTACCGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCgggg                                                                                             <  1:426250/145‑1 (MQ=255)                                                             cATAAATTACCGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGt                                                                                         >  1:501036/1‑149 (MQ=255)                                                                   ttACCGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCACGGGAATTATT‑CCGTGAttaaa                                                                                                                                >  1:666812/1‑100 (MQ=38)                                                                   ttACCGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCACGGGAATTATT‑CCGTGAttaaa                                                                                                                                <  2:666812/104‑5 (MQ=38)                                                                      ccGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGt                                                                                <  1:323529/149‑1 (MQ=255)                                                                      ccGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGt                                                                                <  2:176177/149‑1 (MQ=255)                                                                         aTTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTtat                                                                             >  2:385712/1‑149 (MQ=255)                                                                          ttCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGc                                                                                           >  4:291439/1‑134 (MQ=255)                                                                          ttCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGc                                                                                           <  3:291439/134‑1 (MQ=255)                                                                            cGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAg                                                                          >  1:500837/1‑149 (MQ=255)                                                                                    cAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTAtt                                                                  >  2:625286/1‑149 (MQ=255)                                                                                    cAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTAtt                                                                  <  1:467350/149‑1 (MQ=255)                                                                                    cAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTAtt                                                                  <  4:328427/149‑1 (MQ=255)                                                                                      atatGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCACGGGAATTATT‑CCGTGAttaaa                                                                                                                                >  1:641793/1‑81 (MQ=37)                                                                                      atatGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCACGGGAATTATT‑CCGTGAttaaa                                                                                                                                <  2:641793/85‑5 (MQ=37)                                                                                      atatGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTAttgt                                                                <  2:85693/149‑1 (MQ=255)                                                                                          gAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTgca                                                            >  2:487071/1‑149 (MQ=255)                                                                                            aCAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTgcagg                                                          >  2:243244/1‑149 (MQ=255)                                                                                              aaaCCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTgcaggca                                                        <  4:355222/149‑1 (MQ=255)                                                                                               aaCCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTgcaggcag                                                       <  2:501036/149‑1 (MQ=255)                                                                                               aaCCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTgcaggcag                                                       >  2:72727/1‑149 (MQ=255)                                                                                                aCCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGc                                                                        >  3:337185/1‑131 (MQ=255)                                                                                                aCCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGc                                                                        <  4:337185/131‑1 (MQ=255)                                                                                                         gctgcgcCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTGCAGGCAGTTTGGTCACg                                             <  1:625286/149‑1 (MQ=255)                                                                                                            gcgcCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTGCAGGCAGTTTGGTCACGGcc                                          >  1:76012/1‑149 (MQ=255)                                                                                                                     ggTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTGCAGGCAGTTTGGTCACGGCCAGCGCgcag                                 >  2:691622/1‑149 (MQ=255)                                                                                                                          tGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTGCAGGCAGTTTGGTCACGGCCAGCGCGCAGCAAcc                            >  1:113737/1‑149 (MQ=255)                                                                                                                              tGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTGCAGGCAGTTTGGTCACGGCCAGCGCGCAGCAACCGGAg                        >  3:7008/1‑149 (MQ=255)                                                                                                                                 aaaaGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTAttgtt                                                               <  4:11586/107‑1 (MQ=255)                                                                                                                                 aaaaGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTAttgtt                                                               >  3:11586/1‑107 (MQ=255)                                                                                                                                  aaaGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTGCAGGCAGTTTGGTCACGGCCAGCGCGCAGCAACCGGAGCGTa                    >  1:638473/1‑149 (MQ=255)                                                                                                                                       agGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTGCAGGCAGTTTGGTCACGGCCAGCGCGCAGCAACCGGAGCGTACaaaa               <  4:475497/149‑1 (MQ=255)                                                                                                                                       agGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTGCAGGCAGTTTGGTCACGGCCAGCGCGCAGCAACCGGAGCGTACaaaa               <  1:487071/149‑1 (MQ=255)                                                                                                                                                aaTCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTGCAGGCAGTTTGGTCACGGCCAGCGCGCAGCAACCGGAGCGTACAAAAGTACGTGAg      >  4:461337/1‑149 (MQ=255)                                                                                                                                                 aTCACGGGAATTATT‑CCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGAGCGTAAGTCTGGTTATAAGGCGTTATTGTTGCAGGCAGTTTGGTCACGGCCAGCGCGCAGCAACCGGAGCGTACAAAAGTACGTGAgg     >  2:554400/1‑149 (MQ=255)                                                                                                                                                 aTCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTGCAGGCAGTTTGGTCACGGCCAGCGCGCAGCAACCGGAGCGTACAAAAGTACGTGAgg     >  4:563087/1‑149 (MQ=255)                                                                                                                                                  tCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTGCAGGCAGTTTGGTCACGGCCAGCGCGCAGCAACCGGAGCGTACAAAAGTACGTGAGGa    >  4:329126/1‑149 (MQ=255)                                                                                                                                                  tCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTGCAGGCAGTTTGGTCACGGCCAGCGCGCAGCAACCGGAGCGTACAAAAGTACGTGAGGa    >  2:686836/1‑149 (MQ=255)                                                                                                                                                    aC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTGCAGGCAGTTTGGTCACGGCCAGCGCGCAGCAACCGGAGCGTACAAAAGTACGTGAGGATg  >  2:348197/1‑149 (MQ=255)                                                                                                                                                      |                                                                                                                                                    GAAGTGCTGGAAAAAGAGCTGAAAGTCATGGACCTGGCGGCCTTCACGCTGGCTCGTGACCATAAATTACCGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAATCAC‑GGAATAATTCCCGTGATGGATAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTGCAGGCAGTTTGGTCACGGCCAGCGCGCAGCAACCGGAGCGTACAAAAGTACGTGAGGATG  >  NC_000913/192425‑192720

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑