BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,747,168:1	+C	5.2%	coding (85/1497 nt)	lyxK →	L‑xylulose kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,747,168	1	.	C	5.2%	959.1 / 45.4	213	coding (85/1497 nt)	lyxK	L‑xylulose kinase
Reads supporting (aligned to +/- strand): ref base . (97/105); new base C (5/6); total (102/111)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.15e-01

Only 100 of 219 total aligned reads displayed.

TTTGTTAAAGATGTGCCGCAGGGAGCTGATCTGCTGAAAGCCGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCAT  >  NC_000913/3747021‑3747316

tttgttAAAGATGTGCCGCAGGGAGCTGATCTGCTGAAAGCCGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑t                                                                                                                                                     >  2:265721/1‑149 (MQ=255)

   gttAAAGATGTGCCGCAGGGAGCTGATCTGCTGAAAGCCGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCa                                                                                                                                                  >  3:68954/1‑149 (MQ=255)

     tAAAGATGTGCCGCAGGGAGCTGATCTGCTGAAAGCCGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAgc                                                                                                                                                <  2:190242/149‑1 (MQ=255)

      aaaGATGTGCCGCAGGGAGCTGATCTGCTGAAAGCCGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAgcg                                                                                                                                               >  2:43796/1‑149 (MQ=255)

           tgtgCCGCAGGGAGCTGATCTGCTGAAAGCCGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGCTGCA‑CGCCTGc                                                                                                                                          <  3:237954/149‑1 (MQ=255)

           tgtgCCGCAGGGAGCTGATCTGCTGAAAGCCGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGc                                                                                                                                          <  3:78816/149‑1 (MQ=255)

                cGCAGGGAGCTGATCTGCTGAAAGCCGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCtg                                                                                                                                     <  4:210536/149‑1 (MQ=255)

                   aGGGAGCTGATCTGCTGAAAGCCGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTgc                                                                                                                                  >  4:266830/1‑149 (MQ=255)

                     ggAGCTGATCTGCTGAAAGCCGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTgc                                                                                                                                  <  2:472987/147‑1 (MQ=255)

                       aGCTGATCTGCTGAAAGCCGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCAt                                                                                                                              >  3:23744/1‑149 (MQ=255)

                          tGATCTGCTGAAAGCCGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTaa                                                                                                                           >  1:188199/1‑149 (MQ=255)

                          tGATCTGCTGAAAGCCGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTaa                                                                                                                           >  3:262186/1‑149 (MQ=255)

                          tGATCTGCTGAAAGCCGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTaa                                                                                                                           <  2:551343/149‑1 (MQ=255)

                           gATCTGCTGAAAGCCGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCa                                                                                                                                                  >  3:610510/1‑125 (MQ=255)

                           gATCTGCTGAAAGCCGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAg                                                                                                                          <  4:691173/149‑1 (MQ=255)

                           gATCTGCTGAAAGCCGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAg                                                                                                                          >  1:242269/1‑149 (MQ=255)

                            aTCTGCTGAAAGCCGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGc                                                                                                                                                   <  3:117090/123‑1 (MQ=255)

                            aTCTGCTGAAAGCCGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGc                                                                                                                         >  1:659068/1‑149 (MQ=255)

                            aTCTGCTGAAAGCCGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGc                                                                                                                         >  2:202494/1‑149 (MQ=255)

                             tCTGCTGAAAGCCGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGcc                                                                                                                        <  4:378931/149‑1 (MQ=255)

                              ctgctgAAAGCCGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGCTGCA‑CGCCTGcctcc                                                                                                                                      >  3:156517/1‑131 (MQ=255)

                              ctgctgAAAGCCGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGCTGCA‑CGCCTGcctcc                                                                                                                                      <  4:156517/134‑4 (MQ=255)

                              ctgctgAAAGCCGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGccc                                                                                                                       <  1:676154/149‑1 (MQ=255)

                                  tgAAAGCCGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAg                                                                                                                   >  3:37016/1‑149 (MQ=255)

                                  tgAAAGCCGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAg                                                                                                                   >  2:357010/1‑149 (MQ=255)

                                  tgAAAGCCGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAg                                                                                                                   >  2:221947/1‑149 (MQ=255)

                                   gAAAGCCGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGc                                                                                                                  <  4:611329/149‑1 (MQ=255)

                                    aaaGCCGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGcc                                                                                                                 >  4:175965/1‑149 (MQ=255)

                                       gCCGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAgg                                                                                                              <  4:89368/149‑1 (MQ=255)

                                        ccGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGc                                                                                                             <  3:596723/149‑1 (MQ=255)

                                               tGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCag                                                                                                      >  2:7355/1‑149 (MQ=255)

                                                   gTGCAATAAACCATGACGCAATCCTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGCTGCA‑CGCCTGc                                                                                                                                          <  4:675050/109‑1 (MQ=255)

                                                    tGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCAGTAAGCCCGCAGCCAGGCTGGGCAGAgcgc                                                                                                 >  1:491869/1‑149 (MQ=255)

                                                       aaTAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGat                                                                                              >  1:184522/1‑149 (MQ=255)

                                                       aaTAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGat                                                                                              >  3:455369/1‑149 (MQ=255)

                                                        aTAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGata                                                                                             <  1:451439/149‑1 (MQ=255)

                                                          aaaCCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATg                                                                                           <  1:20/149‑1 (MQ=255)

                                                            aCCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGCAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTaa                                                                                                                           <  3:582279/115‑1 (MQ=255)

                                                            aCCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGTATTaa                                                                                                                           >  4:582279/1‑115 (MQ=255)

                                                             ccATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCa                                                                                        <  3:673274/149‑1 (MQ=255)

                                                              cATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAg                                                                                       >  4:114176/1‑149 (MQ=255)

                                                                 gACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAAc                                                                                    >  3:249469/1‑149 (MQ=255)

                                                                    gCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACtgt                                                                                 <  4:472632/149‑1 (MQ=255)

                                                                     cAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACtgtg                                                                                <  2:327470/149‑1 (MQ=255)

                                                                      aaTACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTgg                                                                               <  1:219192/149‑1 (MQ=255)

                                                                        tACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCa                                                                             <  3:341207/149‑1 (MQ=255)

                                                                            ggctggGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAAtgc                                                                         <  4:37016/149‑1 (MQ=255)

                                                                             gctggGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAAtgct                                                                        >  3:406280/1‑149 (MQ=255)

                                                                             gctggGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAAtgct                                                                        <  1:7355/149‑1 (MQ=255)

                                                                               tggGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCa                                                                                                                >  2:108899/1‑107 (MQ=255)

                                                                               tggGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAAtgctgc                                                                      <  1:308220/149‑1 (MQ=255)

                                                                                ggggTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCa                                                                     <  4:119923/149‑1 (MQ=255)

                                                                                 gggTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCAt                                                                    <  2:318424/149‑1 (MQ=255)

                                                                                 gggTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCAt                                                                    >  3:267500/1‑149 (MQ=255)

                                                                                   gTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATgg                                                                  <  4:239062/149‑1 (MQ=255)

                                                                                    ttAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTgg                                                                               >  2:40884/1‑135 (MQ=255)

                                                                                    ttAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTgg                                                                               <  1:40884/135‑1 (MQ=255)

                                                                                    ttAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGc                                                                 >  3:389539/1‑149 (MQ=255)

                                                                                    ttAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGc                                                                 >  1:549789/1‑149 (MQ=255)

                                                                                    ttAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCAGTATGACCGCGAAGGCCTGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGc                                                                 >  2:530259/1‑149 (MQ=255)

                                                                                     tAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCt                                                                <  1:288661/149‑1 (MQ=255)

                                                                                     tAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCt                                                                <  4:455369/149‑1 (MQ=255)

                                                                                      aGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTg                                                               <  2:659068/149‑1 (MQ=255)

                                                                                              gCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGCCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTcgc                                                       >  1:512063/1‑149 (MQ=255)

                                                                                                 gTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGcc                                                    <  1:644710/149‑1 (MQ=255)

                                                                                                      tggctgAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGaa                                                                                     >  3:259366/1‑111 (MQ=255)

                                                                                                       ggctgAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTcgc                                                       >  3:519784/1‑140 (MQ=255)

                                                                                                        gctgAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTa                                             >  1:542714/1‑149 (MQ=255)

                                                                                                         ctgAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTAc                                            >  1:617470/1‑149 (MQ=255)

                                                                                                          tgAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGc                                                                                                             >  4:61012/1‑83 (MQ=255)

                                                                                                             aaGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCAt                                        >  4:184565/1‑149 (MQ=255)

                                                                                                               gCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTggg                                                                                                         <  1:104493/82‑1 (MQ=255)

                                                                                                               gCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTc                                      <  3:586273/149‑1 (MQ=255)

                                                                                                                  gggCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTgg                                   <  3:175965/149‑1 (MQ=255)

                                                                                                                   ggCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGgt                                  <  4:406280/149‑1 (MQ=255)

                                                                                                                      tGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGgtgt                                >  3:479046/1‑148 (MQ=255)

                                                                                                                          tGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCg                           <  4:258569/149‑1 (MQ=255)

                                                                                                                           gACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCgg                          <  1:134185/149‑1 (MQ=255)

                                                                                                                            aCCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCggg                         <  2:452119/149‑1 (MQ=255)

                                                                                                                             ccGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCgggg                        >  3:107892/1‑149 (MQ=255)

                                                                                                                              cgcgAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGa                       >  1:37425/1‑149 (MQ=255)

                                                                                                                               gcgAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGaa                      <  1:165990/149‑1 (MQ=255)

                                                                                                                               gcgAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGaa                      >  1:253640/1‑149 (MQ=255)

                                                                                                                                cgAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCa                                                                                        >  3:315856/1‑82 (MQ=255)

                                                                                                                                    ggCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAAt                 <  4:320613/149‑1 (MQ=255)

                                                                                                                                       cGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGt              <  2:617470/149‑1 (MQ=255)

                                                                                                                                       cGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGt              >  2:629834/1‑149 (MQ=255)

                                                                                                                                        gggAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTc             <  4:267500/149‑1 (MQ=255)

                                                                                                                                        gggAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTc             <  2:541305/149‑1 (MQ=255)

                                                                                                                                        gggAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTc             <  2:88580/149‑1 (MQ=255)

                                                                                                                                        gggAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGACCGCGATATGGCAGAACTGTGGCAATGCTGCATAGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCgggg                        >  4:507280/1‑138 (MQ=255)

                                                                                                                                         ggAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCg            <  1:459521/149‑1 (MQ=255)

                                                                                                                                          gAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCggg                         >  2:498039/1‑135 (MQ=255)

                                                                                                                                          gAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCggg                         <  1:498039/135‑1 (MQ=255)

                                                                                                                                          gAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCgg           <  2:48474/149‑1 (MQ=255)

                                                                                                                                            ggcaggcG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTa         >  2:58131/1‑149 (MQ=255)

                                                                                                                                             gcaggcGCTGCA‑CGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTAt        >  1:415405/1‑149 (MQ=255)

                                                                                                                                             gcaggcGCTGCA‑CGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTAt        >  3:500161/1‑149 (MQ=255)

                                                                                                                                             gcaggcG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTAt        >  2:173781/1‑149 (MQ=255)

                                                                                                                                               aggcG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCg      <  2:634812/149‑1 (MQ=255)

TTTGTTAAAGATGTGCCGCAGGGAGCTGATCTGCTGAAAGCCGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCAT  >  NC_000913/3747021‑3747316

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`