Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,017,216:1	+A	14.7%	intergenic (+24/‑117)	udp → / → rmuC	uridine phosphorylase/DNA recombination protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,017,216	1	.	A	14.7%	220.9 / 45.8	68	intergenic (+24/‑117)	udp/rmuC	uridine phosphorylase/DNA recombination protein
Reads supporting (aligned to +/- strand):  ref base . (28/30);  new base A (5/5);  total (33/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01

GGTATGGTAGCGGGTGTTATCGTTAACCGCACCCAGCAAGAGATCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCG‑ACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCAATCATGGTTTACG  >  NC_000913/4017070‑4017357                                                                                                                                                    |                                                                                                                                              ggTATGGTAGCGGGTGTTATCGTTAACCGCACCCAGCAAGAGATCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCG‑Ac                                                                                                                                             <  4:109988/149‑1 (MQ=255)        tAGCGGGTGTTATCGTTAACCGCACCCAGCAAGAGATCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCG‑ACGCGTTCg                                                                                                                                      <  1:176581/149‑1 (MQ=255)             ggTGTTATCGTTAACCGCACCCAGCAAGAGATCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCG‑ACGCGTTCGGCCtt                                                                                                                                 <  1:463627/149‑1 (MQ=255)             ggTGTTATCGTTAACCGCACCCAGCAAGAGATCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCG‑ACGCGTTCGGCCtt                                                                                                                                 <  3:541866/149‑1 (MQ=255)              gtgtTATCGTTAACCGCACCCAGCAAGAGATCCCGAATGCTGAGACGATGAAACAAACCGACAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCG‑ACGCGTTCGGCCttt                                                                                                                                >  4:359680/1‑149 (MQ=255)               tgtTATCGTTAACCGCACCCAGCAAGAGATCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGAACGCG‑TCGGCCttca                                                                                                                               <  3:657002/149‑3 (MQ=255)               tgtTATCGTTAACCGCACCCAGCAAGAGATCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCG‑ACGCGTTCGGCCtttt                                                                                                                               <  1:89384/149‑1 (MQ=255)                  tATCGTTAACCGCACCCAGCAAGAGATCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCG‑ACGCGTTCGGCCTTTTGTa                                                                                                                            <  1:113424/149‑1 (MQ=255)                   aTCGTTAACCGCACCCAGCAAGAGATCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCG‑ACGCGTTCGGCCTTTTGTAt                                                                                                                           >  4:582486/1‑149 (MQ=255)                   aTCGTTAACCGCACCCAGCAAGAGATCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCG‑ACGCGTTCGGCCTTTTGTAt                                                                                                                           >  2:519363/1‑149 (MQ=255)                    tCGTTAACCGCACCCAGCAAGAGATCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCG‑ACGCGTTCGGCCTTTTGTAtt                                                                                                                          >  4:299366/1‑149 (MQ=255)                     cGTTAACCGCACCCAGCAAGAGATCCCGAATGCTGAGACGATGAAACAAACCGAAAGCGATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGAACGCG‑TCGGCCttc                                                                                                                                <  3:607364/142‑2 (MQ=255)                     cGTTAACCGCACCCAGCAAGAGATCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTATCTGAAGGCCGAACGCG‑TCGGCCttc                                                                                                                                >  4:607364/1‑141 (MQ=255)                       ttAACCGCACCCAGCAAGAGATCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGAACGCG‑TCGGCCttca                                                                                                                               >  3:99758/1‑139 (MQ=255)                       ttAACCGCACCCAGCAAGAGATCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGAACGCG‑TCGGCCttca                                                                                                                               <  4:99758/141‑3 (MQ=255)                                 ccAGTAAGAGATCCCGAATGCTGAAACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCG‑ACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGcc                                                                                                             >  1:587790/1‑149 (MQ=255)                                 ccAGCAAGAGATCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCG‑Acgc                                                                                                                                           <  1:434240/119‑1 (MQ=255)                                 ccAGCAAGAGATCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCG‑Acgc                                                                                                                                           >  2:434240/1‑119 (MQ=255)                                   aGCAAGAGATCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGTTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCG‑ACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTc                                                                                                           <  2:569253/149‑1 (MQ=255)                                   aGCAAGAGATCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCG‑ACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTc                                                                                                           >  2:684352/1‑149 (MQ=255)                                      aaGAGATCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCG‑ACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCa                                                                                                        <  4:361719/149‑1 (MQ=255)                                        gagaTCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCG‑ACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAgg                                                                                                      >  1:139912/1‑149 (MQ=255)                                           aTCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCG‑ACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGaaa                                                                                                   <  3:299137/149‑1 (MQ=255)                                              ccGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCG‑ACGCGTTCGGCCttt                                                                                                                                >  2:607539/1‑117 (MQ=255)                                              ccGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCG‑ACGCGTTCGGCCttt                                                                                                                                <  1:607539/117‑1 (MQ=255)                                                 aaTGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCG‑ACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCtt                                                                                             <  4:562142/149‑1 (MQ=255)                                                    gCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCG‑ACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTcc                                                                                          <  1:177906/149‑1 (MQ=255)                                                              tGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCG‑ACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGt                                                                                >  3:19898/1‑149 (MQ=255)                                                              tGAAACAAACCGAAAGCCATGCGGTGAAAAACGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCG‑ACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGt                                                                                >  1:509832/1‑149 (MQ=255)                                                                aaacaaacCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCG‑ACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGttt                                                                              <  2:179708/149‑1 (MQ=255)                                                                aaacaaacCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCG‑ACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGttt                                                                              <  4:24188/149‑1 (MQ=255)                                                                   caaacCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCG‑ACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTa                                                                           <  2:293293/149‑1 (MQ=255)                                                                    aaacCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCG‑ACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGaa                                                                                                    <  1:135399/123‑1 (MQ=255)                                                                    aaacCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCG‑ACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGaa                                                                                                    >  2:135399/1‑123 (MQ=255)                                                                    aaacCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCG‑ACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTAc                                                                          >  3:348442/1‑149 (MQ=255)                                                                            aGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCG‑ACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGAc                                                                                  <  2:411420/133‑1 (MQ=255)                                                                            aGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCG‑ACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGAc                                                                                  >  1:411420/1‑133 (MQ=255)                                                                                    ggTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCG‑ACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTAtttt                                                          <  4:19898/149‑1 (MQ=255)                                                                                           aTCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCG‑ACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCggg                                                   >  3:276215/1‑149 (MQ=255)                                                                                           aTCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCG‑ACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCggg                                                   <  2:139912/149‑1 (MQ=255)                                                                                                    gAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCG‑ACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAgttgtt                                          <  3:296380/149‑1 (MQ=255)                                                                                                         ggcgCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCG‑ACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTc                                     >  2:66628/1‑149 (MQ=255)                                                                                                          gcgcGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCG‑ACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCa                                    >  4:237870/1‑149 (MQ=255)                                                                                                          gcgcGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCG‑ACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCa                                    <  3:582486/149‑1 (MQ=255)                                                                                                             cgtcgtCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCG‑ACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCagga                                 <  1:457601/149‑1 (MQ=255)                                                                                                              gtcgtcTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCG‑ACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCaggag                                <  3:440246/149‑1 (MQ=255)                                                                                                                  tcTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCG‑ACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGt                            <  2:453016/149‑1 (MQ=255)                                                                                                                    tgctgTAATTCTCTTCTCCTGTCTGAAGGCCG‑ACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTtg                          >  2:198644/1‑149 (MQ=255)                                                                                                                     gctgTAATTCTCTTCTCCTGTCTGAAGGCCG‑ACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTtgt                         <  1:684352/149‑1 (MQ=255)                                                                                                                     gctgTAATTCTCTTCTCCTGTCTGAAGGCCG‑ACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTtgt                         <  1:198644/149‑1 (MQ=255)                                                                                                                       tgTAATTCTCTTCTCCTGTCTGAAGGCCG‑ACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTgg                       >  2:330527/1‑149 (MQ=255)                                                                                                                         tAATTCTCTTCTCCTGTCTGAAGGCCG‑ACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGtt                           <  2:528896/143‑1 (MQ=255)                                                                                                                         tAATTCTCTTCTCCTGTCTGAAGGCCG‑ACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGtt                           >  1:528896/1‑143 (MQ=255)                                                                                                                           aTTCTCTTCTCCTGTCTGAAGGCCG‑ACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGAttt                   >  1:545573/1‑149 (MQ=255)                                                                                                                             tctcttctcCTGTCTGAAGGCCG‑ACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTtct                 >  4:92241/1‑149 (MQ=255)                                                                                                                                 ttctcCTGTCTGAAGGCCG‑ACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCAAt             <  1:66628/149‑1 (MQ=255)                                                                                                                                   ctcCTGTCTGAAGGCCG‑ACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCAATCa           >  4:427721/1‑149 (MQ=255)                                                                                                                                    tcCTGTCTGAAGGCCG‑ACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCAATCAt          <  2:587790/149‑1 (MQ=255)                                                                                                                                     ccTGTCTGAAGGCCG‑ACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCAATCATg         >  1:318236/1‑149 (MQ=255)                                                                                                                                       tGTCTGAAGGCCG‑ACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCAATCATGGt       >  4:437597/1‑149 (MQ=255)                                                                                                                                           tGAAGGCCG‑ACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCAATCATGGTTTAc   >  2:134703/1‑149 (MQ=255)                                                                                                                                            gAAGGCCG‑ACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACGGCACAATTCTACTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCAATCATGGTTTACg  >  2:494211/1‑149 (MQ=255)                                                                                                                                            gAAGGCCG‑ACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCAATCATGGTTTACg  >  3:500098/1‑149 (MQ=255)                                                                                                                                            aaaGGCCGAACGCG‑TCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCAATCATGGTTTACg  >  3:341565/2‑149 (MQ=255)                                                                                                                                             aaGGCCGAACGCG‑TCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTaa                                                <  4:218226/102‑1 (MQ=255)                                                                                                                                             aaGGCCGAACGCG‑TCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTaa                                                >  3:218226/1‑102 (MQ=255)                                                                                                                                               ggCCGAACGCG‑TCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCagga                                 <  2:46873/115‑1 (MQ=255)                                                                                                                                               ggCCGAACGCG‑TCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCagga                                 >  1:46873/1‑115 (MQ=255)                                                                                                                                                    |                                                                                                                                              GGTATGGTAGCGGGTGTTATCGTTAACCGCACCCAGCAAGAGATCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCG‑ACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCAATCATGGTTTACG  >  NC_000913/4017070‑4017357

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑