Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,020,510	G→T	6.4%	intergenic (+21/‑30)	ssnA → / → ygfM	putative chlorohydrolase/aminohydrolase/putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,020,510	0	G	T	6.4%	219.4 / 5.2	62	intergenic (+21/‑30)	ssnA/ygfM	putative chlorohydrolase/aminohydrolase/putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (25/33);  new base T (2/2);  total (27/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.67e-01

ACAGCGTGATGGTCAATGGTGTGATGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCC‑GCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAATGCTACACCA  >  NC_000913/3020362‑3020653                                                                                                                                                      |                                                                                                                                                acaGCGTGATGGTCAATGGTGTGATGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCC‑g                                                                                                                                                 >  3:66011/1‑149 (MQ=255)  caGCGTGATGGTCAATGGTGTGATGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCC‑Gc                                                                                                                                                >  4:383275/1‑149 (MQ=255)   aGCGTGATGGTCAATGGTGTGATGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCC‑GCa                                                                                                                                               <  4:443215/149‑1 (MQ=255)     cGTGATGGTCAATGGTGTGATGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCC‑GCAgg                                                                                                                                             <  1:496387/149‑1 (MQ=255)          tGGTCACTGGTGTGATGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGTATAAATGACAGATGCCCTCTTCCC‑GCAGGGAtga                                                                                                                                        <  3:459723/149‑1 (MQ=255)          tGGTCAATGGTGTGATGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCC‑TGCGGGAaga                                                                                                                                        <  3:595513/149‑1 (MQ=255)          tGGTCAATGGTGTGATGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCC‑GCAGGGAaga                                                                                                                                        <  1:99112/149‑1 (MQ=255)           ggTCAATGGTGTGATGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCTGC‑GGGAagag                                                                                                                                       <  4:7965/149‑1 (MQ=255)            gTCAATGGTGTGATGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCTGC‑GGGAAGAgg                                                                                                                                      <  1:385265/149‑1 (MQ=255)            gTCAATGGTGTGATGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCTGC‑GGGAAGAgg                                                                                                                                      <  3:613297/149‑1 (MQ=255)            gTCAATGGTGTGATGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCC‑TGCGGGAAGAgg                                                                                                                                      <  1:220422/149‑1 (MQ=255)               aaTGGTGTGATGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCC‑GCAGGGAAGAGGGtt                                                                                                                                   <  2:81986/149‑1 (MQ=255)               aaTGGTGTGATGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCC‑GCAGGGAAGAGGGtt                                                                                                                                   >  4:175774/1‑149 (MQ=255)                  ggTGTGATGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCC‑GCAGGGAAGAGGGTTaaa                                                                                                                                <  4:298921/149‑1 (MQ=255)                      tgATGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCC‑GCAGGGAAGAGGGTTAAATAAg                                                                                                                            <  3:246706/149‑1 (MQ=255)                       gATGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCC‑GCAGGGAAGAGGGTTAAATAAgg                                                                                                                           <  1:114976/149‑1 (MQ=255)                            tCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCC‑GCAGGGAaga                                                                                                                                        <  2:482739/131‑1 (MQ=255)                            tCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCC‑GCAGGGAaga                                                                                                                                        >  1:482739/1‑131 (MQ=255)                             cTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCC‑GCAGGGAAGAGGGTTAAATAAGGAaagaa                                                                                                                     <  4:395057/149‑1 (MQ=255)                                 gAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGTATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCC‑GCAGGGAAGAGGGTTAAATAAGGAAAGAAgatg                                                                                                                 >  4:79299/1‑149 (MQ=255)                                  aaGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCC‑GCAGGGAAGAGGGTTAAATAAGGAAAGAAgatga                                                                                                                >  4:283210/1‑149 (MQ=255)                                    gACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGTATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCC‑GCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGAtt                                                                                                              <  3:79299/149‑1 (MQ=255)                                            gTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCC‑GCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAAtt                                                                                                      >  2:121878/1‑149 (MQ=255)                                              ttAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCC‑GCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAAtttt                                                                                                    <  3:550248/149‑1 (MQ=255)                                                 aCTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCC‑GCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCa                                                                                                 <  3:417551/149‑1 (MQ=255)                                                   ttCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCC‑GCAGGGAAGAGGGtt                                                                                                                                   >  3:390509/1‑113 (MQ=255)                                                   ttCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCC‑GCAGGGAAGAGGGtt                                                                                                                                   <  4:390509/113‑1 (MQ=255)                                                   ttCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCC‑GCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAgg                                                                                               >  4:614939/1‑149 (MQ=255)                                                   ttCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCC‑GCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAgg                                                                                               >  3:539007/1‑149 (MQ=255)                                                     cGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCC‑GCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGcc                                                                                             <  3:175774/149‑1 (MQ=255)                                                               tCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCC‑GCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTc                                                                                   <  4:66011/149‑1 (MQ=255)                                                               tCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCC‑GCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTc                                                                                   <  3:383275/149‑1 (MQ=255)                                                                  aTTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCC‑GCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGaa                                                                                >  1:154960/1‑149 (MQ=255)                                                                   tttATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCC‑GCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAAc                                                                               >  1:564984/1‑149 (MQ=255)                                                                      aTGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCC‑GCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAgg                                                                            <  1:121878/149‑1 (MQ=255)                                                                          acaAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCC‑GCAGGGAAGAGGGt                                                                                                                                    <  2:72533/89‑1 (MQ=255)                                                                          acaAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCC‑GCAGGGAAGAGGGt                                                                                                                                    >  1:72533/1‑89 (MQ=255)                                                                              gCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCC‑GCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCa                                                                                                 <  3:509955/120‑1 (MQ=255)                                                                              gCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCC‑GCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCa                                                                                                 >  4:509955/1‑120 (MQ=255)                                                                              gCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCC‑GCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGaa                                                                    >  3:616979/1‑149 (MQ=255)                                                                                cAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCC‑GCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCGGGCCCGACTCTGTCGAACAGGCGCTGGAACt                                                                  >  3:622013/1‑149 (MQ=255)                                                                                   aaaaGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCC‑GCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGaa                                                               <  1:264546/149‑1 (MQ=255)                                                                                     aaGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCC‑GCAGGGAAGAGGGTTAAATAAGGAAAGGAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAgc                                                             <  2:564984/149‑1 (MQ=255)                                                                                     aaGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCC‑GCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAgc                                                             <  3:283210/149‑1 (MQ=255)                                                                                        ccGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCC‑GCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGcgcc                                                          >  2:210850/1‑149 (MQ=255)                                                                                         cGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCC‑GCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCGGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGcgccg                                                         <  4:622013/149‑1 (MQ=255)                                                                                            tGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCC‑GCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTa                                                      >  2:150929/1‑149 (MQ=255)                                                                                                   aTGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCC‑GCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGAt                                               <  2:378635/149‑1 (MQ=255)                                                                                                                         tGGCATAAATGACAGATGCCCTCTTCCC‑GCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCgggg                         <  4:616979/149‑1 (MQ=255)                                                                                                                           gCATAAATGACAGATGCCCTCTTCCC‑GCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGgc                       >  1:365034/1‑149 (MQ=255)                                                                                                                             aTAAATGACAGATGCCCTCTTCCC‑GCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGgcag                     >  1:309165/1‑149 (MQ=255)                                                                                                                                 aTGACAGATGCCCTCTTCCC‑GCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCaaa                 <  3:614939/149‑1 (MQ=255)                                                                                                                                        aTGCCCTCTTCCC‑GCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGAt                                               <  4:574027/112‑1 (MQ=255)                                                                                                                                        aTGCCCTCTTCCC‑GCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGAt                                               >  3:574027/1‑112 (MQ=255)                                                                                                                                         tGCCCTCTTCCC‑GCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAATg          <  2:223453/148‑1 (MQ=255)                                                                                                                                         tGCCCTCTTCCC‑GCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAATg          >  1:223453/1‑148 (MQ=255)                                                                                                                                            ccTCTTCCCTGC‑GGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAATGCTac      >  3:317860/1‑149 (MQ=255)                                                                                                                                            ccTCTTCCC‑TGCGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCTCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAATGCTac      >  4:192429/1‑149 (MQ=255)                                                                                                                                            ccTCTTCCC‑TGCGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAATGCTac      >  4:289600/1‑149 (MQ=255)                                                                                                                                            ccTCTTCCC‑GCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCa                                                                                                 <  2:177625/58‑1 (MQ=255)                                                                                                                                            ccTCTTCCC‑GCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCa                                                                                                 >  1:177625/1‑58 (MQ=255)                                                                                                                                             ctctTCCC‑GCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAATGCTaca     >  1:252713/1‑149 (MQ=255)                                                                                                                                                ttCCC‑GCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAATGCTACACCa  >  1:408396/1‑149 (MQ=255)                                                                                                                                                      |                                                                                                                                                ACAGCGTGATGGTCAATGGTGTGATGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCC‑GCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAATGCTACACCA  >  NC_000913/3020362‑3020653

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑