| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 558,762 | C→A | 6.5% | intergenic (+8/‑212) | sfmA → / → sfmC | FimA homolog, function unknown/putative periplasmic pilus chaperone |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 558,762 | 0 | C | A | 6.5% | 266.0 / 7.9 | 77 | intergenic (+8/‑212) | sfmA/sfmC | FimA homolog, function unknown/putative periplasmic pilus chaperone |
| Reads supporting (aligned to +/- strand): ref base C (34/38); new base A (2/3); total (36/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.68e-01 | |||||||||||
GAATAGCTTCTCAACCAACCAGAACTTGATCCCCGGGACCAACGTTCTTCATTTTTCTGCACGTTATAAAGGCACCGGTACAAGTGCATCAGCAGGGCAAGCAAATGCTGACGCGACTTTTATTATGAGATATGAATAA‑TCAAAACCACGTTGTTTTGAATTATATATCACGTCTTATAACAAAGTAATGTACCGGTTGTCTGAAGCGGTATGGTGGCAATGTAAATCGAAATCATGTTCACTTTGTATCATGCCGCTTTATTAAATGAAAAGGGAATGATGTGTTGT > NC_000913/558616‑558903 | gAATAGCTTCTCAACCAACCAGAACTTGATCCCCGGGACCAACGTTCTTCATTTTTCTGCACGTTATAAAGGCACCGGTACAAGTGCATCAGCAGGGCAAGCAAATGCTGACGCGACTTTTATTATGAGATATGAATAA‑TCAAAACCAc > 2:173194/1‑149 (MQ=255) cTTCTCAACCAACCAGAACTTGATCCCCGGGACCAACGTTCTTCATTTTTCTGCACGTTATAAAGGCACCGGTACAAGTGCATCAGCAGGGCAAGCAAATGCTGACGCGACTTTTATTATGAGATATGAATAA‑TCAAAACCACgttgtt > 2:284800/1‑149 (MQ=255) caaccaacCAGAACTTGATCCCCGGGACCAACGTTCTTCATTTTTCTGCACGTTATAAAGGCACCGGTACAAGTGCATCAGCAGGGCAAGCAAATGCTGACGCGACTTTTATTATGAGATATGAATAA‑TCAAAACCACGTTGTTTTGaa < 2:681765/149‑1 (MQ=255) caaccaacCAGAACTTGATCCCCGGGACCAACGTTCTTCATTTTTCTGCACGTTATAAAGGCACCGGTACAAGTGCATCAGCAGGGCAAGCAAATGCTGACGCGACTTTTATTATGAGATATGAATAA‑TCAAAACCACGTTGTTTTGaa < 3:18949/149‑1 (MQ=255) aaccaaccAGAACTTGATCCCCGGGACCAACGTTCTTCATTTTTCTGCACGTTATAAAGGCACCGGTACAAGTGCATCAGCAGGGCAAGCAAATGCTGACGCGACTTTTATTATGAGATATGAATAA‑TCAAAACCACGTTGTTTTGAAt > 4:357103/1‑149 (MQ=255) ccaaccaGAACTTGATCCCCGGGACCAACGTTCTTCATTTTTCTGCACGTTATAAAGGCACCGGTACAAGTGCATCAGCAGGGCAAGCAAATGCTGACGCGACTTTTATTATGAGATATGAATAA‑TCAAAACCACGTTGTTTTGAATta > 4:288621/1‑149 (MQ=255) ccaaccaGAACTTGATCCCCGGGACCAACGTTCTTCATTTTTCTGCACGTTATAAAGGCACCGGTACAAGTGCATCAGCAGGGCAAGCAAATGCTGACGCGACTTTTATTATGAGATATGAATAA‑TCAAAACAACGTGGTTTTGattat < 1:232411/149‑5 (MQ=255) aaccaGAACTTGATCCCCGGGACCAACGTTCTTCATTTTTCTGCACGTTATAAAGGCACCGGTACAAGTGCATCAGCAGGGCAAGCAAATGCTGACGCGACTTTTATTATGAGATATGAATAA‑TCAAAACCACGTTGTTTTGAATtata < 4:132437/149‑1 (MQ=255) aaccaGAACTTGATCCCCGGGACCAACGTTCTTCATTTTTCTGCACGTTATAAAGGCACCGGTACAAGTGCATCAGCAGGGCAAGCAAATGCTGACGCGACTTTTATTATGAGATATGAATAA‑TCAAAACCACGTTGTTTTGAATtata < 3:357103/149‑1 (MQ=255) aaccaGAACTTGATCCCCGGGACCAACGTTCTTCATTTTTCTGCACGTTATAAAGGCACCGGTACAAGTGCATCAGCAGGGCAAGCAAATGCTGACGCGACTTTTATTATGAGATATGAATAA‑TCAAAACCACGTTGTTTTGAATtata < 1:173194/149‑1 (MQ=255) aaccaGAACTTGATCCCCGGGACCAACGTTCTTCATTTTTCTGCACGTTATAAAGGCACCGGTACAAGTGCATCAGCAGGGCAAGCAAATGCTGACGCGACTTTTATTATGAGATATGAATAA‑TCAAAACAACGTGGTTTTGa > 4:575307/1‑143 (MQ=255) aaccaGAACTTGATCCCCGGGACCAACGTTCTTCATTTTTCTGCACGTTATAAAGGCACCGGTACAAGTGCATCAGCAGGGCAAGCAAATGCTGACGCGACTTTTATTATGAGATATGAATAA‑TCAAAACAACGTGGTTTTGa < 3:575307/143‑1 (MQ=255) caGAACTTGATCCCCGGGACCAACGTTCTTCATTTTTCTGCACGTTATAAAGGCACCGGTACAAGTGCATCAGCAGGGCAAGCAAATGCTGACGCGACTTTTATTATGAGATATGAATAA‑TCAAAACCACGTTGTTTTGAATTatatat > 1:345949/1‑149 (MQ=255) caGAACTTGATCCCCGGGACCAACGTTCTTCATTTTTCTGCACGTTATAAAGGCACCGGTACAAGTGCATCAGCAGGGCAAGCAAATGCTGACGCGACTTTTATTATGAGATATGAATAA‑TCAAAACCACGTTGTTTTGAATTatatat > 2:37165/1‑149 (MQ=255) aGAACTTGATCCCCGGGACCAACGTTCTTCATTTTTCTGCACGTTATAAAGGCACCGGTACAAGTGCATCAGCAGGGCAAGCAAATGCTGACGCGACTTTTATTATGAGATATGAATAA‑TCAAAACCACGTTGTTTTGAATTATATATc < 1:98819/149‑1 (MQ=255) gAACTTGATCCCCGGGACCAACGTTCTTCATTTTTCTGCACGTTCTAAAGGCACCGGTACAAGTGCATCAGCAGGGCAAGCAAATGCTGACGCGACTTTTATTATGAGATATGAATAA‑TCAAAACCACGTTGTTTTGAATTATATATCa < 3:439727/149‑1 (MQ=255) cTTGATCCCCGGGACCAACGTTCTTCATTTTTCTGCACGTTATAAAGGCACCGGTACAAGTGCATCAGCAGGGCAAGCAAATGCTGACGCGACTTTTATTATGAGATATGAATAA‑TCAAAACCACGTTGTTTTGAATTATATATCACGt < 2:691816/149‑1 (MQ=255) ttGATCCCCGGGACCAACGTTCTTCATTTTTCTGCACGTTATAAAGGCACCGGTACAAGTGCATCAGCAGGGCAAGCAAATGCTGACGCGACTTTTATTATGAGATATGAATAA‑TCAAAACCACGTTGTTTTGAATta > 4:171739/1‑138 (MQ=255) ttGATCCCCGGGACCAACGTTCTTCATTTTTCTGCACGTTATAAAGGCACCGGTACAAGTGCATCAGCAGGGCAAGCAAATGCTGACGCGACTTTTATTATGAGATATGAATAA‑TCAAAACCACGTTGTTTTGAATta < 3:171739/138‑1 (MQ=255) ttGATCCCCGGGACCAACGTTCTTCATTTTTCTGCACGTTATAAAGGCACCGGTACAAGTGCATCAGCAGGGCAAGCAAATGCTGACGCGACTTTTATTATGAGATATGAATAA‑TCAAAACCACGTTGTTTTGAATTATATATCACGTc < 1:160381/149‑1 (MQ=255) tCCCCGGGACCAACGTTCTTCATTTTTCTGCACGTTATAAAGGCACCGGTACAAGTGCATCAGCAGGGCAAGCAAATGCTGACGCGACTTTTATTATGAGATATGAATAA‑TCAAAACCACGTTGTTTTGAATTATATATCACGTCTtat > 2:141964/1‑149 (MQ=255) ccccGGGACCAACGTTCTTCATTTTTCTGCACGTTATAAAGGCACCGGTACAAGTGCATCAGCAGGGCAAGCAAATGCTGACGCGACTTTTATTATGAGATATGAATAA‑TCAAAACCACGTTGTTTTGAATTATATATCACGTCTtata < 1:37165/149‑1 (MQ=255) ggACCAACGTTCTTCATTTTTCTGCACGTTATAAAGGCACCGGTACAAGTGCATCAGCAGGGCAAGCAAATGCTGACGCGACTTTTATTATGAGATATGAATAA‑TCAAAACCACGTTGTTTTGAATTATATATCACGTCTTATAACaaa < 4:290667/149‑1 (MQ=255) cAACGTTCTTCATTTTTCTGCACGTTATAAAGGCACCGGTACAAGTGCATCAGCAGGGCAAGCAAATGCTGACGCGACTTTTATTATGAGATATGAATAA‑TCAAAACCACGTTGTTTTGAATTATATATCACGTCTTATAACAAAGTaa < 4:478566/149‑1 (MQ=255) aCGTTCTTCATTTTTCTGCACGTTATAAAGGCACCGGTACAAGTGCATCAGCAGGGCAAGCAAATGCTGACGCGACTTTTATTATGAGATATGAATAA‑TCAAAACCACGTTGTTTTGAATTATATATCACGTCTTATAACAAAGTAATg > 1:59667/1‑149 (MQ=255) aCGTTCTTCATTTTTCTGCACGTTATAAAGGCACCGGTACAAGTGCATCAGCAGGGCAAGCAAATGCTGACGCGACTTTTATTATGAGATATGAATAA‑TCAAAACCACGTTGTTTTGAATTATATATCACGTCTTATAACAAAGTAATg > 3:603852/1‑149 (MQ=255) cttcATTTTTCTGCACGTTATAAAGGCACCGGTACAAGTGCATCAGCAGGGCAAGCAAATGCTGACGCGACTTTTATTATGAGATATGAATAATTCAAAACAACGTGGTTTTGattattc > 1:685253/1‑114 (MQ=38) cttcATTTTTCTGCACGTTATAAAGGCACCGGTACAAGTGCATCAGCAGGGCAAGCAAATGCTGACGCGACTTTTATTATGAGATATGAATAATTCAAAACAACGTGGTTTTGattattc < 2:685253/120‑7 (MQ=38) cttcATTTTTCTGCACGTTATAAAGGCACCGGTACAAGTGCATCAGCAGGGCAAGCAAATGCTGACGCGACTTTTATTATGAGATATGAATAA‑TCAAAACCACGTTGTTTTGAATTATATATCACGTCTTATAACAAAGTAATGTACCg > 3:294857/1‑149 (MQ=255) ttcATTTTTCTGCACGTTATAAAGGCACCGGTACAAGTGCATCAGCAGGGCAAGCAAATGCTGACGCGACTTTTATTATGAGATATGAATAA‑TCAAAACCACGTTGTTTTGAATTATATATCACGTCTTATAACAAAGTAATGTACCgg > 1:75501/1‑149 (MQ=255) ttCTGCACGTTATAAAGGCACCGGTACAAGTGCATCAGCAGGGCAAGCAAATGCTGACGCGACTTTTATTATGAGATATGAATAA‑TCAAAACCACGTTGTTTTGAATTATATATCACGTCTTATAACAAAGTaa > 4:304932/1‑134 (MQ=255) ttCTGCACGTTATAAAGGCACCGGTACAAGTGCATCAGCAGGGCAAGCAAATGCTGACGCGACTTTTATTATGAGATATGAATAA‑TCAAAACCACGTTGTTTTGAATTATATATCACGTCTTATAACAAAGTaa < 3:304932/134‑1 (MQ=255) tGCACGTTATAAAGGCACCGGTACAAGTGCATCAGCAGGGCAAGCAAATGCTGACGCGACTTTTATTATGAGATATGAATAA‑TCAAAACCACGTTGTTTTGAATTATATATCACGTCTTATAACAAAGTAATGTACCGGTTGTCTGAAg > 4:549306/1‑149 (MQ=255) aCGTTATAAAGGCACCGGTACAAGTGCATCAGCAGGGCAAGCAAATGCTGACGCGACTTTTATTATGAGATATGAATAA‑TCAAAACCACGTTGTTTTGAATTATATATCACGTCTTATAACAAAGTAATGTACCGGTTGTCTGAAGCgg > 3:419855/1‑149 (MQ=255) aCGTTATAAAGGCACCGGTACAAGTGCATCAGCAGGGCAAGCAAATGCTGACGCGACTTTTATTATGAGATATGAATAA‑TCAAAACCACGTTGTTTTGAATTATATATCACGTCTTATAACAAAGTAATGTACCGGTTGTCTGAAGCgg > 2:264920/1‑149 (MQ=255) aaaGGCACCGGTACAAGTGCATCAGCAGGGCAAGCAAATGCTGACGCGACTTTTATTATGAGATATGAATAA‑TCAAAACCACGTTGTTTTGAATTATATATCACGTCTTATAACAAAGTAATGTACCGGTTGTCTGAAGCGGTAtggtg < 4:603852/149‑1 (MQ=255) aaGGCACCGGTACAAGTGCATCAGCAGGGCAAGCAAATGCTGACGCGACTTTTATTATGAGATATGAATAA‑TCAAAACCACGTTGTTTTGAATTATATATCACGTCTTATAACAAAGTAAt < 2:680452/121‑1 (MQ=255) aaGGCACCGGTACAAGTGCATCAGCAGGGCAAGCAAATGCTGACGCGACTTTTATTATGAGATATGAATAA‑TCAAAACCACGTTGTTTTGAATTATATATCACGTCTTATAACAAAGTAAt > 1:680452/1‑121 (MQ=255) aGGCACCGGTACAAGTGCATCAGCAGGGCAAGCAAATGCTGACGCGACTTTTATTATGAGATATGAATAA‑TCAAAACCACGTTGTTTTGAATTATATATCACGTCTTATAACAAAGTAATGTACCGGTTGTCTGAAGCGGTATGATGGc > 2:7157/1‑149 (MQ=255) ggCACCGGTACAAGTGCATCAGCAGGGCAAGCAAATGCTGACGCGACTTTTATTATGAGATATGAATAA‑TCAAAACCACGTTGTTTTGAATTATATATCACGTCTTATAACAAAGTaa > 2:312572/1‑118 (MQ=255) ggCACCGGTACAAGTGCATCAGCAGGGCAAGCAAATGCTGACGCGACTTTTATTATGAGATATGAATAA‑TCAAAACCACGTTGTTTTGAATTATATATCACGTCTTATAACAAAGTaa < 1:312572/118‑1 (MQ=255) ggCACCGGTACAAGTGCATCAGCAGGGCAAGCAAATGCTGACGCGACTTTTATTATGAGATATGAATAA‑TCAAAACCACGTTGTTTTGAATTATATATCACGTCTTATAACAAAGTAATGTACCGGTTGTCTGAAGCGGTATGGTGGCa > 3:513495/1‑149 (MQ=255) ccGGTACAAGTGCATCAGCAGGGCAAGCAAATGCTGACGCGACTTTTATTATGAGATATGAATAA‑TCAAAACCACGTTGTTTTGAATTATATATCACGTCTTATAACAAAGTAATGTACCGGTTGTCTGAAGCGGTATGGTGGCAATGt < 3:549306/149‑1 (MQ=255) cGGTACAAGTGCATCAGCAGGGCAAGCAAATGCTGACGCGACTTTTATTATGAGATATGAATAA‑TCAAAACCACGTTGTTTTGAATTATATATCACGTCTta > 4:258174/1‑102 (MQ=255) cGGTACAAGTGCATCAGCAGGGCAAGCAAATGCTGACGCGACTTTTATTATGAGATATGAATAA‑TCAAAACCACGTTGTTTTGAATTATATATCACGTCTta < 3:258174/102‑1 (MQ=255) tACAAGTGCATCAGCAGGGCAAGCAAATGCTGACGCGACTTTTATTATGAGATATGAATAA‑TCAAAACCACGTTGTTTTGAATTATATATCACGTCTTATAACAAAGTAATGTACCGGTTGTCTGAAGCGGTATGGCGGCAATGTAAAt < 1:223908/149‑1 (MQ=255) aaGTGCATCAGCAGGGCAAGCAAATGCTGACGCGACTTTTATTATGAGATATGAATAA‑TCAAAACCACGTTGTTTTGAATTATATATCACGTCTTATAACAAAGTAATGTACCGGTTGTCTGAAGCGGTATGGTGGCAATGTAAATCGa > 2:616457/1‑149 (MQ=255) aaGTGCATCAGCAGGGCAAGCAAATGCTGACGCGACTTTTATTATGAGATATGAATAA‑TCAAAACCACGTTGTTTTGAATTATATATCACGTCTTATAACAAAGTAATGTACCGGTTGTCTGAAGCGGTATGGTGGCAATGTAAATCGa > 2:94574/1‑149 (MQ=255) aGTGCATCAGCAGGGCAAGCAAATGCTGACGCGACTTTTATTATGAGATATGAATAA‑TCAAAACCACGTTGTTTTGAATTATATATCACGTCTTATAACAAAGTAATGTACCGGTTGTCTGAAGCGGTATGGTGGCAATGTAAATCGaa < 2:345949/149‑1 (MQ=255) tCAGCAGGGCAAGCAAATGCTGACGCGACTTTTATTATGAGATATGAATAA‑TCAAAACCACGTTGTTTTGAATTATATATCACGTCTTATAACAAAGTAATGTACCGGTTGTCTGAAGCGGTATGGTGGCAATGTAAATCGAAATCATg > 3:300518/1‑149 (MQ=255) agcagGGCAAGCAAATGCTGACGCGACTTTTATTATGAGATATGAATAA‑TCAAAACCACGTTGTTTTGAATTATATATCACGTCTTATAACAAAGTAATGTACCGGTTGTCTGAAGCGGTATGGTGGCAATGTAAATCGAAATCATGtt < 2:75501/149‑1 (MQ=255) gcagGGCAAGCAAATGCTGACGCGACTTTTATTATGAGATATGAATAA‑TCAAAACCACGTTGTTTTGAATTATATATCACGTCTTATAACaaa > 1:607622/1‑93 (MQ=255) gcagGGCAAGCAAATGCTGACGCGACTTTTATTATGAGATATGAATAA‑TCAAAACCACGTTGTTTTGAATTATATATCACGTCTTATAACaaa < 2:607622/93‑1 (MQ=255) gggCAAGCAAATGCTGACGCGACTTTTATTATGAGATATGTATAA‑TCAAAACCACGTTGTTTTGAATTATATATCACGTCTTATAACAAAGTAATGTACCGGTTGTCTGAAGCGGTATGGTGGCAATGTAAATCGAAATCATGTTCACt > 4:71407/1‑149 (MQ=255) ggCAAGCAAATGCTGACGCGACTTTTATTATGAGATATGAATAA‑TCAAAACCACGTTGTTTTGAATTATATATCACGTCTTATAACAAAGTAATGTACCGGTTGTCTGAAGCGGTATGGTGGCAATGTAAATCGAAATCATGTTCACtt < 1:94574/149‑1 (MQ=255) gcaagcaaATGCTGACGCGACTTTTATTATGAGATATGAATAA‑TCAAAACCACGTTGTTTTGAATTATATATCACGTCTTATAACAAAGTAATGTACCGGTTGTCTGAAGCGGTATGGTGGCAATGTAAATCGAAATCATGTGCACttt < 3:673572/149‑1 (MQ=255) aagcaaATGCTGACGCGACTTTTATTATGAGATATGAATAA‑TCAAAACCACGTTGTTTTGAATTATATATCACGTCTTATAACAAAGTAATGTACCGGTTGTCTGAAGCGGTATGGTGGCAATGTAAATCGAAATCATGTTCACTTTGt < 4:294857/149‑1 (MQ=255) aaaTGCTGACGCGACTTTTATTATGAGATATGTATAA‑TCAAAACCACGTTGTTTTGAATTATATATCACGTCTTATAACAAAGTAATGTACCGGTTGTCTGAAGCGGTATGGTGGCAATGTAAATCGAAATCATGTTCACTTTGTATCa < 3:71407/149‑1 (MQ=255) gCTGACGCGACTTTTATTATGAGATATGAATAA‑TCAAAACCACGTTGTTTTGAATTATATATCACGTCTTATAACAAAGTAATGTACCGGTTGTCTGAAGCGGTATGGTGGCAATGTAAATCGAAATCATGTTCACTTTGTATCATGcc < 1:302578/149‑1 (MQ=255) cgcgACTTTTATTATGAGATATGAATAA‑TCAAAACCACGTTGTTTTGAATTATATATCACGTCTTATAACAAAGTAATGTACCGGTTGTCTGAAGCGGTATGGTGGCAATGTAAATCGAAATCATGTTCACTTTGTATCATGCCGCttt < 1:616457/149‑1 (MQ=255) aCTTTTATTATGAGATATGAATAA‑TCAAAACCACGTTGTTTTGAATTATATATCACGTCTTATAACAAAGTAATGTACCGGTTGTCTGAAGCGGTATGGTGGCAATGTAAATCGAAATCATGTTCACTTTGTATCATGCCGCTttatta > 1:457104/1‑149 (MQ=255) ttttATTATGAGATATGAATAA‑TCAAAACCACGTTGTTTTGAATTATATATCACGTCTTATAACAAAGTAATGTACCGGTTGTCTGAAGCGGTATGGTGGCAATGTAAATCGAAATCATGTTCACTTTGt > 2:674842/1‑130 (MQ=255) ttttATTATGAGATATGAATAA‑TCAAAACCACGTTGTTTTGAATTATATATCACGTCTTATAACAAAGTAATGTACCGGTTGTCTGAAGCGGTATGGTGGCAATGTAAATCGAAATCATGTTCACTTTGt < 1:674842/130‑1 (MQ=255) ttttATTATGAGATATGAATAA‑TCAAAACCACGTTGTTTTGAATTATATATCACGTCTTATAACAAAGTAATGTACCGGTTGTCTGAAGCGGTATGGTGGCAATGTAAATCGAAATCATGTTCACTTTGTATc < 3:342145/133‑1 (MQ=255) ttttATTATGAGATATGAATAA‑TCAAAACCACGTTGTTTTGAATTATATATCACGTCTTATAACAAAGTAATGTACCGGTTGTCTGAAGCGGTATGGTGGCAATGTAAATCGAAATCATGTTCACTTTGTATc > 4:342145/1‑133 (MQ=255) ttttATTATGAGATATGAATAA‑TCAAAACCACGTTGTTTTGAATTATATATCACGTCTTATAACAAAGTAATGTACCGGTTGTCTGAAGCGGTATGGTGGCAATGTAAATCGAAATCATGTTCACTTTGTATCATGCCGCTTTATTaaa < 1:141964/149‑1 (MQ=255) ttttATTATGAGATATGAATAA‑TCAAAACCACGTTGTTTTGAATTATATATCACGTCTTATAACAAAGTAATGTACCGGTTGTCTGAAGCGGTATGGTGGCAATGTAAATCGAAATCATGTTCACTTTGTATCATGCCGCTTTATTaaa > 2:479748/1‑149 (MQ=255) ttttATTATGAGATATGAATAA‑TCAAAACCACGTTGTTTTGAATTATATATCACGTCTTATAACAAAGTAATGTACCGGTTGTCTGAAGCGGTATGGTGGCAATGTAAATCGAAATCATGTTCACTTTGTATCATGCCGCTTTATTaaa < 1:479748/149‑1 (MQ=255) ttatGAGATATGAATAA‑TCAAAACCACGTTGTTTTGAATTATATATCACGTCTTATAACAAAGTAATGTACCGGTTGTCTGAAGCGGTATGGTGGCAATGTAAATCGAAATCATGTTCACTTTGTATCATGCCGCTTTATTAAATGaaa > 3:445693/1‑149 (MQ=255) ttatGAGATATGAATAA‑TCAAAACCACGTTGTTTTGAATTATATATCACGTCTTATAACAAAGTAATGTACCGGTTGTCTGAAGCGGTATGGTGGCAATGTAAATCGAAATCATGTTCACTTTGTATCATGCCGCTTTATTAAATGaaa > 1:477738/1‑149 (MQ=255) atGAGATATGAATAA‑TCAAAACCACGTTGTTTTGAATTATATATCACGTCTTATAACAAAGTAATGTACCGGTTGTCTGAAGCGGTATGGTGGCAATGTAAATCGAAATCATGTTCACTTTGTATCATGCCGCTTTATTAAATGAAAAg < 3:326698/149‑1 (MQ=255) tGAGATATGAATAA‑TCAAAACCACGTTGTTTTGAATTATATATCACGTCTTATAACAAAGTAATGTACCGGTTGTCTGAAGCGGTATGGTGGCAATGTAAATCGAAATCATGTTCACTTTGTATCATGCCGCTTTATTAAATGAAAAgg < 4:419855/149‑1 (MQ=255) agaTATGAATAA‑TCAAAACCACGTTGTTTTGAATTATATATCACGTCTTATAACAAAGTAATGTACCGGTTGTCTGAAGCGGTATGGTGGCAATGTAAATCGAAATCATGTTCACTTTGTATCATGCCGCTTTATTAAATGAAAAGGGa < 4:445693/149‑1 (MQ=255) gAATAA‑TCAAAACCACGTTGTTTTGAATTATATATCACGTCTTATAACAAAGTAATGTACCGGTTGTCTGAAGCGGTATGGTGGCAATGTAAATCGAAATCATGTTCACTTTGTATCATGCCGCTTTATTAAATGAAAAGGGAatgatg < 3:288621/149‑1 (MQ=255) aa‑tCAAAACCACGTTGTTTTGAATTATATATCACGTCTTATAACAAAGTAATGTACCGGTTGTCTGAAGCGGTATGGTGGCAATGTAAATCGAAATCATGTTCACTTTGTATCATGCCGCTTTATTAAATGAAAAGGGAATGATGtgtt < 4:513495/149‑1 (MQ=255) a‑tCAAAACCACGTTGTTTTGAATTATATATCACGTCTTATAACAAAGTAATGTACCGGTTGTCTGAAGCGGTATGGTGGCAATGTAAATCGAAATCATGTTCACTTTGTATCATGCCGCTTTATTAAATGAAAAGGGAATGATGtgttg > 4:381921/1‑149 (MQ=255) tCAAAACCACGTTGTTTTGAATTATATATCACGTCTTATAACAAAGTAATGTACCGGTTGTCTGAAGCGGTATGGTGGCAATGTAAATCGAAATCATGTTCACTTTGTATCATGCCGCTTTATTAAATGAAAAGGGAATGATGtgttgt > 2:371730/1‑149 (MQ=255) | GAATAGCTTCTCAACCAACCAGAACTTGATCCCCGGGACCAACGTTCTTCATTTTTCTGCACGTTATAAAGGCACCGGTACAAGTGCATCAGCAGGGCAAGCAAATGCTGACGCGACTTTTATTATGAGATATGAATAA‑TCAAAACCACGTTGTTTTGAATTATATATCACGTCTTATAACAAAGTAATGTACCGGTTGTCTGAAGCGGTATGGTGGCAATGTAAATCGAAATCATGTTCACTTTGTATCATGCCGCTTTATTAAATGAAAAGGGAATGATGTGTTGT > NC_000913/558616‑558903 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |