| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 120,158:1 | +A | 9.5% | intergenic (+23/+20) | ampE → / ← aroP | ampicillin resistance inner membrane protein; putative signaling protein in beta‑lactamase regulation/aromatic amino acid transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 120,158 | 1 | . | A | 9.5% | 321.4 / 31.5 | 84 | intergenic (+23/+20) | ampE/aroP | ampicillin resistance inner membrane protein; putative signaling protein in beta‑lactamase regulation/aromatic amino acid transporter |
| Reads supporting (aligned to +/- strand): ref base . (37/39); new base A (5/3); total (42/42) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.13e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.18e-01 | |||||||||||
CTGGCGCGTGAACCGCATGTCGATAAGGTGGAGACGCCGAAGGCAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTAC‑GGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAGGTATACCGAAATCGCCATTCCTGGGGTCATCAGCATAATCACCA > NC_000913/120010‑120302 | ctGGCGCGTGAACCGCATGTCGATAAGGTGGAGACGCCGAAGGCAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTAc < 4:91337/149‑1 (MQ=255) ggCGCGTGAACCGCATGTCGATAAGGTGGAGACGCCGAAGGCAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTAC‑gg < 4:659297/149‑1 (MQ=255) ggCGCGTGAACCGCATGTCGATAAGGTGGAGACGCCGAAGGCAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTAC‑gg < 1:129756/149‑1 (MQ=255) ggCGCGTGAACCGCATGTCGATAAGGTGGAGACGCCGAAGGCAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTAC‑gg < 2:608777/149‑1 (MQ=255) ggCGCGTGAACCGCATGTCGATAAGGTGGAGACGCCGAAGGCAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTAC‑gg < 1:780890/149‑1 (MQ=255) gTGAACCGCATGTCGATAAGGTGGAGACGCCGAAGGCAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTAC‑GGGTGAg < 4:119565/149‑1 (MQ=255) gTGAACCGCATGTCGATAAGGTGGAGACGCCGAAGGCAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTAC‑GGGTGAg < 3:574906/149‑1 (MQ=255) gTGAACCGCATGTCGATAAGGTGGAGACGCCGAAGGCAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTAC‑GGGTGAg < 1:483862/149‑1 (MQ=255) tGAACCGCATGTCGATAAGGTGGAGACGCCGAAGGCAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTAC‑GGGTGAgg < 3:820249/149‑1 (MQ=255) tGAACCGCATGTCGATAAGGTGGAGACGCCGAAGGCAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCC‑GTACAGGGTGAgg < 2:365269/149‑1 (MQ=255) tGAACCGCATGTCGATAAGGTGGAGACGCCGAAGGCAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCC‑GTACAGGGTGAgg < 1:798715/149‑1 (MQ=255) aaCCGCATGTCGATAAGGTGGAGACGCCGAAGGCAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTAC‑GGGTGAGGGc > 3:755289/1‑149 (MQ=255) ccGCATGTCGATAAGGTGGAGACGCCGAAGGCAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTAC‑GGGTGAGGGCGt < 3:878944/149‑1 (MQ=255) ccGCATGTCGATAAGGTGGAGACGCCGAAGGCAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTAC‑GGGTGAGGGCGt > 2:794510/1‑149 (MQ=255) cGCATGTCGATAAGGTGGAGACGCCGAAGGCAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTAC‑GGGTGAGGGCGTa > 2:887598/1‑149 (MQ=255) gATAAGGTGGAGACGCCGAAGGCAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCGCCCTGAAC‑GGGTGAGGGCGTAGAGAGAtt < 4:19969/149‑1 (MQ=255) aGGTGGAGACGCCGAAGGCAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTAC‑GGGTGAGGGCGTAGAGAGATTAATg > 3:843665/1‑149 (MQ=255) ggTGGAGACGCCGAAGGCAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTAC‑GGGTGAGGGCGTAGAGAGATTAATgc < 1:598261/149‑1 (MQ=255) agaCGCCGAAGGCAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTAC‑GGGTGAGGGCGTAGAGAGATTAATGCGCttt > 3:88832/1‑149 (MQ=255) agaCGCAGATGGCAGCGGTTTCAATGGCGCAGAAACCCTCGTTCTTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTCCCCTCATCCTGTAC‑GGGTGAGGGCGTAGAGAGATTAATGCGCttt < 4:150156/149‑1 (MQ=255) gaCGCCGAAGGCAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTAC‑GGGTGAGGGCGTa < 1:18984/130‑1 (MQ=255) gaCGCCGAAGGCAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTAC‑GGGTGAGGGCGTa > 2:18984/1‑130 (MQ=255) aCGCCGAAGGCAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTAC‑GGGTGAGGGCGTAGAGAGATTAATGCGCTTTTa > 3:799501/1‑149 (MQ=255) cGCCGAAGGCAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTAC‑GGGTGAGGGCGTAGAGAGATTAATGCGCTTTTAc < 1:201638/149‑1 (MQ=255) cGCCGAAGGCAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTAC‑GGGTGAGGGCGTAGAGAGATTAATGCGCTTTTAc < 4:788172/149‑1 (MQ=255) gCCGAAGGCAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTAC‑GGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACg > 2:895741/1‑149 (MQ=255) aaGGCAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTAC‑GGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCtt < 4:799501/149‑1 (MQ=255) aaGGCAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTAC‑GGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCtt > 3:822335/1‑149 (MQ=255) aGGCAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTAC‑GGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCttt < 4:375868/149‑1 (MQ=255) tttCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTAC‑GGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGtttt > 2:67539/1‑149 (MQ=255) cAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTAC‑GGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTtctc < 1:887598/149‑1 (MQ=255) aaTGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTAC‑GGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTctct < 3:266994/149‑1 (MQ=255) tGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTAC‑GGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCttt < 1:291632/149‑1 (MQ=255) gCGAAGAAAACCTCGTTTGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTTAAGATTATTGCCCTCACCCTGTAC‑GGGTGTGGGCGTAGAGAGATTAATGCGCTTTTACGGTTTTGGCGGTTTTCTCTTTaa > 4:755165/1‑149 (MQ=255) cGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTAC‑GGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTaaa < 1:660447/149‑1 (MQ=255) gaagaaAACCTAGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTAC‑GGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAAc > 4:147285/1‑149 (MQ=255) cccTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTAC‑GGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGc < 1:502086/148‑1 (MQ=255) ccTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGAGGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTAC‑GGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGcc < 2:471997/149‑1 (MQ=255) cTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTAC‑GGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCg > 1:670048/1‑149 (MQ=255) cTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTAC‑GGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCg > 3:95895/1‑149 (MQ=255) cTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTAC‑GGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCg > 3:638925/1‑149 (MQ=255) tcgttcgtGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTAC‑GGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGa < 1:832513/149‑1 (MQ=255) gttcgtGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTAC‑GGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATa < 1:895741/149‑1 (MQ=255) gtggtcgtggtGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTAC‑GGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTa > 2:865213/1‑149 (MQ=255) gtcgtggtGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTAC‑GGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAaca > 4:705711/1‑149 (MQ=255) gtggtgATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTAC‑GGGTGAGGGCGTAGAGAGATTAATGCTCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCa < 4:512038/149‑1 (MQ=255) gtggtgATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTAC‑GGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCa < 4:843665/149‑1 (MQ=255) gtggtgATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTAC‑GGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCa < 4:88832/149‑1 (MQ=255) ggtgATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTAC‑GGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGc > 1:58341/1‑149 (MQ=255) ggtgATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTAC‑GGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGc < 4:857902/149‑1 (MQ=255) ggtgATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTAC‑GGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGc < 1:865213/149‑1 (MQ=255) gATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTAC‑GGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCAt > 1:221622/1‑149 (MQ=255) actactGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTAC‑GGGTGAGGGCGTAGAGAGATTAATGCGCTTTTCCGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCggg > 4:733006/1‑149 (MQ=255) actactGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTAC‑GGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCggg > 4:170971/1‑149 (MQ=255) tactGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTAC‑GGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGAt > 4:243246/1‑149 (MQ=255) ctGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTAC‑GGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCa < 3:705711/149‑1 (MQ=255) tGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTAC‑GGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAg > 3:323577/1‑149 (MQ=255) gACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTAC‑GGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAgg > 1:51222/1‑149 (MQ=255) gACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTAC‑GGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAgg > 4:492576/1‑149 (MQ=255) gACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTAC‑GGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAgg > 1:5486/1‑149 (MQ=255) aCGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTAC‑GGGTGAGGGCGTAGAGAGATTAATgcgc < 2:520995/75‑1 (MQ=255) aCGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTAC‑GGGTGAGGGCGTAGAGAGATTAATgcgc > 1:520995/1‑75 (MQ=255) aCGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTAC‑GGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAGGt > 1:168543/1‑149 (MQ=255) tttACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTAC‑GGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAGGTATAc > 1:754613/1‑149 (MQ=255) tttACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTAC‑GGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAGGTATAc < 4:421270/149‑1 (MQ=255) ttACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTAC‑GGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAGGTATAcc < 1:794510/149‑1 (MQ=255) gTTGGTGTAAAGATTATTGCCCTCACCCTGTAC‑GGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAGGTATACCGAAATCGcc < 1:56558/149‑1 (MQ=255) tAAAGATTATTGCCCTCACCCTGTAC‑GGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAGGTATACCGAAATCGCCATTCCTg > 1:759850/1‑149 (MQ=255) aaaGATTATTGCCCTCACCCTGTAC‑GGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAGGTATACCGAAATCGCCATTCCTgg < 3:792816/149‑1 (MQ=255) aaGATTATTGCCCTCACCCTGTAC‑GGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAGGTATACCGAAATCGCCATTCCTggg > 3:580941/1‑149 (MQ=255) aaGATTATTGCCCTCACCCTGTAC‑GGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAGGTATACCGAAATCGCCATTCCTggg > 3:462500/1‑149 (MQ=255) aaGATTATTGCCCTCACCCTGTAC‑GGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAGGTATACCGAAATCGCCATTCCTggg > 3:779100/1‑149 (MQ=255) attGCCCTCACCCTGTAC‑GGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAGGTATACCGAAATCGCCATTCCTGGGGtcatc < 1:67539/149‑1 (MQ=255) gCCCTCACCCTGTAC‑GGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAGGTATACCGAAATCGCCATTCCTGGGGTCATCAGc < 2:129645/149‑1 (MQ=255) gCCCTCACCCTGTAC‑GGGTGAGAGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACTGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAGGTATACTGAAATCGCCATTCCTGGGGTCATCAGc > 2:833486/1‑149 (MQ=255) gCCCTCACCC‑GTACAGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAGGTATACCGAAATCGCCATTCCTGGGGTCATCAGc > 2:135450/1‑149 (MQ=255) cccTCACCCTGTAC‑GGGTGAGGGCGTAGAGAGATTAAGGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAGGTATACCGAAATCGCCATTCCTGGGGTCATCAGCa < 3:210486/149‑1 (MQ=255) ccTCACCCTGTAC‑GGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAGGTATACCGAAATCGCCATTCCTGGGGtcatca < 2:381321/145‑1 (MQ=255) ccTCACCCTGTAC‑GGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAGGTATACCGAAATCGCCATTCCTGGGGtcatca > 1:381321/1‑145 (MQ=255) ccTCACCC‑GTACAGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTaa < 1:134186/89‑1 (MQ=39) ccTCACCC‑GTACAGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTaa > 2:134186/1‑89 (MQ=39) ccTCACCC‑GTACAGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAGGTATACCGAAATCGCCATTCCTGGGGTCATCAGCAt > 3:425195/1‑149 (MQ=255) ccTCACCC‑GTACAGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAGGTATACCGAAATCGCCATTCCTGGGGTCATCAGCAt > 1:692778/1‑149 (MQ=255) ccTCACCC‑GTACAGGGTGAGGGCGTAGAGAGATTAATGCGCTCTTACGGCTTTGGCGGTTTTCACTTTAAACAGATAGCCGATGCCTAACACGATCAGCCATACCGGGATCAGGTATACCGAAATCGCTATTCCTGGGGTCGTCAGCAt > 4:622199/1‑149 (MQ=255) aCCCTGTAC‑GGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAGGTATACCGAAATCGCCATTCCTGGGGTCATCAGCATAATc < 4:621829/149‑1 (MQ=255) cTGTAC‑GGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATAcc > 2:64256/1‑98 (MQ=255) cTGTAC‑GGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATAcc < 1:64256/98‑1 (MQ=255) cTGTAC‑GGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAGGTATACCGAAATCGCCATTCCTGGGGTCATCAGCATAATCAcc > 4:85913/1‑149 (MQ=255) tGTAC‑GGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAGGTATACCGAAATCGCCATTCCTGGGGTCATCAGCATAATCACCa < 3:243246/149‑1 (MQ=255) | CTGGCGCGTGAACCGCATGTCGATAAGGTGGAGACGCCGAAGGCAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTAC‑GGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAGGTATACCGAAATCGCCATTCCTGGGGTCATCAGCATAATCACCA > NC_000913/120010‑120302 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |