| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,901,038:1 | +T | 10.0% | coding (548/1557 nt) | yoaE ← | putative membrane protein/conserved protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,901,038 | 1 | . | T | 10.0% | 184.0 / 17.2 | 50 | coding (548/1557 nt) | yoaE | putative membrane protein/conserved protein |
| Reads supporting (aligned to +/- strand): ref base . (28/17); new base T (2/3); total (30/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.77e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.41e-01 | |||||||||||
ACGCGCAATCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGG‑GGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTACCGCATCCAACGAGAAGACGGCGT > NC_000913/1900890‑1901179 | aCGCGCAATCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTgg > 4:82733/1‑149 (MQ=255) cgcgCAATCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTgg‑g < 1:421551/149‑1 (MQ=255) aaTCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACC‑CCACGGTGGTGGTGCt < 2:275671/149‑1 (MQ=38) aTCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGG‑GGTGCTg < 4:260058/149‑1 (MQ=255) aTCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACC‑CCACGGTGGTGGTGCtc < 4:531722/149‑2 (MQ=255) ttAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGG‑GGTGCTGGTTAAc < 2:241982/149‑1 (MQ=255) ttCGATGATGATCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGG‑GGTGCTGGTTAACGAATcgcg > 4:29473/1‑149 (MQ=255) ttCGATGATGATCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATAATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGG‑GGTGCTGGTTAACGAATcgcg > 1:323359/1‑149 (MQ=255) tgatgatCGAGAAGCCAATCGCGGCATACAGGTAACCTATCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGG‑GGTGCTGGTTAACGAATCGCGTCAGc < 4:389813/149‑1 (MQ=255) gatgatCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACC‑CCACGGTGGTGGTGCtc > 1:402653/1‑128 (MQ=255) gatgatCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACC‑CCACGGTGGTGGTGCtc < 2:402653/129‑2 (MQ=37) atCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGTATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGG‑GGTGCTGGTTAACGAATCGCGTCAGCGGttt > 4:236496/1‑149 (MQ=255) atCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGG‑GGTGCTGGTTAACGAATCGCGTCAGCGGttt < 4:412843/149‑1 (MQ=255) tCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGG‑GGTGCTGGTTAACGAATCGCGTCAGCGGTTTg > 2:495680/1‑149 (MQ=255) gagaAGCCAATCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGG‑GGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGa < 3:328835/149‑1 (MQ=255) gCCAATCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGG‑GGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCa > 2:93123/1‑149 (MQ=255) aaTCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGG‑GGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCa > 1:250883/1‑149 (MQ=255) aTCGCGGCATACAGGTAACCTTTCGGTATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGG‑GGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGa < 4:381737/140‑1 (MQ=255) aTCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGG‑GGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGa > 3:381737/1‑140 (MQ=255) agTAACCTTTCGGAATGTGGGAACCGAAACCTTCTGCCACCAGAATCAGACCACTCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGG‑GGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCCGCAACATAACCGCGATc > 4:118632/2‑149 (MQ=39) aCCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGG‑GGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCaa < 1:57243/149‑1 (MQ=255) tttCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGG‑GGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCa > 4:503132/1‑149 (MQ=255) gAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGG‑GGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTAcc > 1:316163/1‑149 (MQ=255) gaaaccgaaaccTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGG‑GGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCca > 2:196409/1‑149 (MQ=255) cgaaaccTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCTGACAGAGCACCACCACCGTGG‑GGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCTGCAACATAGCCGCCATCGCAATCACTACCGCCGCCatcatc > 4:350672/1‑149 (MQ=255) cgaaaccTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGG‑GGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAAcc < 2:364275/119‑1 (MQ=255) cgaaaccTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGG‑GGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAAcc > 1:364275/1‑119 (MQ=255) gaaaccTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGG‑GGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCAtcatca > 1:525036/1‑149 (MQ=255) aaaccTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGG‑GGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCAc > 4:539283/1‑149 (MQ=255) ttCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGG‑GGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCa < 1:93123/149‑1 (MQ=255) ttCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGG‑GGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCa > 2:237833/1‑149 (MQ=255) accaGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGG‑GGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGtt > 2:247380/1‑149 (MQ=255) ccaGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGG‑GGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTa < 2:333628/149‑1 (MQ=255) gACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGG‑GGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAAcc < 4:246929/149‑1 (MQ=255) tCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGG‑GGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATc > 4:71483/1‑149 (MQ=255) cAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGG‑GGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATcc < 1:495680/149‑1 (MQ=255) cAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGG‑GGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGcc > 1:79489/1‑80 (MQ=255) cAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGG‑GGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGcc < 2:79489/80‑1 (MQ=255) aCAGGAAGCTCAGACAGAGCACCACCACCGTGG‑GGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAAcc > 4:397078/1‑83 (MQ=255) aCAGGAAGCTCAGACAGAGCACCACCACCGTGG‑GGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAAcc < 3:397078/83‑1 (MQ=255) aCAGGAAGCTCAGACAGAGCACCACCACCGTGG‑GGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAAtt < 2:127810/149‑1 (MQ=255) ggAAGCTCAGACAGAGCACCACCACCGTGG‑GGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTAcc > 1:330698/1‑149 (MQ=255) tCAGACAGAGCACCACCACCGTGG‑GGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTAc < 2:175548/142‑1 (MQ=255) tCAGACAGAGCACCACCACCGTGG‑GGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTAc > 1:175548/1‑142 (MQ=255) cagacagaGCACCACCACCGTGG‑GGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTACCGCATCCa > 3:247541/1‑149 (MQ=255) ttaaccagCACC‑CCACGGTGGTGGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTACCGCATCCaa > 2:570520/7‑149 (MQ=255) agagCACCACCACCGTGG‑GGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTACCGCATCCAACgag > 3:149011/1‑149 (MQ=255) agagCACCACCACCGTGG‑GGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTACCGCATCCAACgag > 2:268304/1‑149 (MQ=255) agagCACCACCACCGTGG‑GGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTACCGCAACCAACgag < 3:539283/149‑1 (MQ=255) accaccaccGTGG‑GGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTACCGCATCCAACGAGAAGAc > 3:218548/1‑149 (MQ=255) ccaccGTGG‑GGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTACCGCATCCAACGAGAAGACGGCg > 1:323296/1‑149 (MQ=255) caccGTGG‑GGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTACCGCATCCAACGAGAAGACGGCGt > 3:47394/1‑149 (MQ=255) | ACGCGCAATCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGG‑GGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTACCGCATCCAACGAGAAGACGGCGT > NC_000913/1900890‑1901179 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |