Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,276,886 C→A 10.7% intergenic (+33/‑116) garD → / → prlF D‑galactarate dehydrogenase/antitoxin of the SohA(PrlF)‑YhaV toxin‑antitoxin system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,276,8860CA10.7% 284.5 / 19.8 84intergenic (+33/‑116)garD/prlFD‑galactarate dehydrogenase/antitoxin of the SohA(PrlF)‑YhaV toxin‑antitoxin system
Reads supporting (aligned to +/- strand):  ref base C (41/34);  new base A (5/4);  total (46/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.55e-01

CTGGAAGTTGTTCCACTTTATTCTCGACGTCGCCAGCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGC‑CTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGAATCATGCCCGCTAATGCTCGCTCTCACGC  >  NC_000913/3276739‑3277027
                                                                                                                                                    |                                                                                                                                             
cTGGAAGTTGTTCCACTTTATTCTCGACGTCGCCAGCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGC‑Ct                                                                                                                                              >  3:55602/1‑149 (MQ=255)
    aaGTTGTTCCACTTTATTCTCGACGTCGCCAGCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGC‑CTTAGt                                                                                                                                          <  4:55602/149‑1 (MQ=255)
      gttgttCCACTTTATTCTCGACGTCGCCAGCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGC‑CTTAGTGc                                                                                                                                        >  4:460185/1‑149 (MQ=255)
      gttgttCCACTTTATTCTCGACGTCGCCAGCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGC‑CTTAGTGc                                                                                                                                        >  4:565476/1‑149 (MQ=255)
          ttCCACTTTATTCTCGACGTCGCCAGCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGC‑CTTAGTGCCATg                                                                                                                                    >  2:267093/1‑149 (MQ=255)
           tCCACTTTATTCTCGACGTCGCCAGCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGC‑CTTAGTGCCATGt                                                                                                                                   >  2:512331/1‑149 (MQ=255)
           tCCACTTTATTCTCGACGTCGCCAGCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGC‑ACTAAGGCCATGt                                                                                                                                   <  3:251663/149‑1 (MQ=255)
            ccACTTTATTCTCGACGTCGCCAGCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGC‑CTTAGTGCCATGtt                                                                                                                                  >  2:496317/1‑149 (MQ=255)
             cACTTTATTCTCGACGTCGCCAGCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGC‑CTTAGTGCCATGttt                                                                                                                                 >  1:189567/1‑149 (MQ=255)
             cACTTTATTCTCGACGTCGCCAGCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGC‑CTTAGTGCCATGttt                                                                                                                                 >  4:324145/1‑149 (MQ=255)
              aCTTTATTCTCGACGTCGCCAGCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCACTAAG‑GCCATGtttt                                                                                                                                >  3:131383/1‑149 (MQ=255)
               cTTTATTCTCGACGTCGCCAGCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGC‑ACTAAGGCCATGttttg                                                                                                                               <  4:131383/149‑2 (MQ=255)
                    ttCTCGACGTCGCCAGCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGC‑CTTAGTGCCATGTTTTTATTGt                                                                                                                          >  3:532431/1‑149 (MQ=255)
                      ctcGACGTCGCCAGCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGC‑CTTAGTGCCATGTTTTTATTGttt                                                                                                                        <  1:184079/149‑1 (MQ=255)
                       tcGACGTCGCCAGCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGC‑CTTAGTGCCATGTTTTTATTGTTTa                                                                                                                       >  4:79355/1‑149 (MQ=255)
                        cGACGTCGCCAGCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGC‑CTTAGTGCCATGTTTTTATTGTTTaa                                                                                                                      <  3:339715/149‑1 (MQ=255)
                         gACGTCGCCAGCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGC‑CTTAGTGCCATGTTTTTATTGTTTaaa                                                                                                                     <  4:532431/149‑1 (MQ=255)
                         gACGTCGCCAGCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGC‑CTTAGTGCCATGTTTTTATTGTTTaaa                                                                                                                     <  2:212541/149‑1 (MQ=255)
                            gTCGCCAGCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGC‑CTTAGTGCCATGTTTTTATTGTTTAAAGcc                                                                                                                  <  1:496317/149‑1 (MQ=255)
                            gTCGCCAGCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGC‑CTTAGTGCCATGTTTTTATTGTTTAAAGcc                                                                                                                  >  1:72659/1‑149 (MQ=255)
                            gTCGCCAGCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGC‑CTTAGTGCCATGTTTTTATTGTTTAAAGcc                                                                                                                  <  4:463966/149‑1 (MQ=255)
                                 cAGCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGC‑CTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCAc                                                                                                             >  4:494018/1‑149 (MQ=255)
                                      ggAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGC‑CTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCa                                                                                                        >  4:560146/1‑149 (MQ=255)
                                      ggAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGC‑CTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCa                                                                                                        >  4:154977/1‑149 (MQ=255)
                                           aagaaAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGC‑CTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATtaat                                                                                                   >  3:55651/1‑149 (MQ=255)
                                            agaaAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGC‑CTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATtaata                                                                                                  <  1:101382/149‑1 (MQ=255)
                                             gaaAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGC‑CTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATtaataa                                                                                                 >  3:318867/1‑149 (MQ=255)
                                             gaaAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCCGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAGACATGGC‑CTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATtaataa                                                                                                 <  4:383674/149‑1 (MQ=255)
                                              aaaaCCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGC‑CTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTaataat                                                                                                <  3:560146/149‑1 (MQ=255)
                                              aaaaCCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGC‑CTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTaataat                                                                                                <  3:154977/149‑1 (MQ=255)
                                                aaCCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGC‑CTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAAtgc                                                                                              >  2:293211/1‑149 (MQ=255)
                                                       tcGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGC‑CTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAAtgcatttgca                                                                                       <  3:565476/149‑1 (MQ=255)
                                                           aTCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGC‑CTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTAc                                                                                   <  3:519209/149‑1 (MQ=255)
                                                           aTCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGC‑CTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTAc                                                                                   >  2:305806/1‑149 (MQ=255)
                                                              aaTGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGC‑CTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCAAATGCATTattaa                                                                                <  4:318867/149‑5 (MQ=255)
                                                               aTGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGC‑CTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTa                                                                               <  3:369692/149‑1 (MQ=255)
                                                               aTGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGC‑CTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTa                                                                               >  4:570368/1‑149 (MQ=255)
                                                                   gggCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGC‑CTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTc                                                                           >  2:400194/1‑149 (MQ=255)
                                                                   gggCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGC‑CTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTc                                                                           >  3:427598/1‑149 (MQ=255)
                                                                     gCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGC‑CTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCaa                                                                         <  1:51076/149‑1 (MQ=255)
                                                                      cTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGC‑CTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAg                                                                        <  3:79355/149‑1 (MQ=255)
                                                                      cTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGC‑CTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAg                                                                        <  1:400194/149‑1 (MQ=255)
                                                                      cTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGC‑ACTAAGGCCATGttt                                                                                                                                 >  4:304707/1‑92 (MQ=38)
                                                                      cTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGC‑ACTAAGGCCATGttt                                                                                                                                 <  3:304707/92‑1 (MQ=38)
                                                                                  cTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGC‑CTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGa                                                            <  4:55651/149‑1 (MQ=255)
                                                                                  cTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGC‑CTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGa                                                            <  4:586193/149‑1 (MQ=255)
                                                                                   tGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGC‑CTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAg                                                           <  1:12781/149‑1 (MQ=255)
                                                                                   tGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGC‑CTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAg                                                           <  4:128046/149‑1 (MQ=255)
                                                                                     gCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGC‑CTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCa                                                         >  1:192230/1‑149 (MQ=255)
                                                                                       ggTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGC‑CTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGa                                                       >  3:428080/1‑149 (MQ=255)
                                                                                        gtgtTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGC‑CTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGaa                                                      >  1:317379/1‑149 (MQ=255)
                                                                                           tttAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGC‑CTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAAc                                                   >  1:108253/1‑149 (MQ=255)
                                                                                            ttAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGC‑CTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACa                                                  <  2:317379/149‑1 (MQ=255)
                                                                                                 ccGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGC‑CTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTg                                             >  4:249035/1‑149 (MQ=255)
                                                                                                      aCCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGC‑CTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTaaaa                                        <  3:373378/149‑1 (MQ=255)
                                                                                                           tGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGC‑CTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACa                                   <  3:207667/149‑1 (MQ=255)
                                                                                                              ccTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGC‑CTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTg                                >  3:586655/1‑149 (MQ=255)
                                                                                                               cTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGC‑CTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGa                               >  4:183728/1‑149 (MQ=255)
                                                                                                               cTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGC‑CTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGa                               >  2:150901/1‑149 (MQ=255)
                                                                                                                tGATTTCTTTTGGAACGTCCCTCGCAAAACATGGC‑CTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGaa                              >  2:250790/1‑149 (MQ=255)
                                                                                                                 gATTTCTTTTGGAACGTCCCTCGCAAAACATGGC‑CTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGAAt                             <  3:183728/149‑1 (MQ=255)
                                                                                                                  aTTTCTTTTGGAACGTCCCTCGCAAAACATGGC‑CTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCTCGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGAATc                            <  3:460185/149‑1 (MQ=255)
                                                                                                                  aTTTCTTTTGGAACGTCCCTCGCAAAACATGGC‑CTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGAATc                            <  2:192230/149‑1 (MQ=255)
                                                                                                                      cTTTTGGAACGTCCCTCGCAAAACATGGC‑CTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGAATCATGc                        <  3:249035/149‑1 (MQ=255)
                                                                                                                       ttttGGAACGTCCCTCGCAAAACATGGC‑CTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGAAt                             >  1:27725/1‑143 (MQ=255)
                                                                                                                       ttttGGAACGTCCCTCGCAAAACATGGC‑CTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGAAt                             <  2:27725/143‑1 (MQ=255)
                                                                                                                       ttttGGAACGTCCCTCGCAAAACATGGC‑CTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGAATCATGcc                       >  4:293521/1‑149 (MQ=255)
                                                                                                                       ttttGGAACGTCCCTCGCAAAACATGGC‑CTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGAATCATGcc                       >  1:448185/1‑149 (MQ=255)
                                                                                                                           ggAACGTCCCTCGCAAAACATGGC‑CTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGAATCATGCCCGCt                   >  4:358489/1‑149 (MQ=255)
                                                                                                                                  aacaataaaaaCATGGC‑ACTAAGGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGAATCATGCCCGCTAATgctc            >  3:417742/8‑149 (MQ=255)
                                                                                                                                   ccTCGCAAAACATGGC‑CTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGAATCATGCCCGCTAATgctcg           >  1:217615/1‑149 (MQ=255)
                                                                                                                                     tCGCAAAACATGGC‑CTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGAATCATGCCCGCTAATgctcgct         >  3:374788/1‑149 (MQ=255)
                                                                                                                                     tCGCAAAACATGGC‑CTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGAATCATGCCCGCTAATgctcgct         >  3:339695/1‑149 (MQ=255)
                                                                                                                                      cGCAAAACATGGC‑CTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGAATCATGCCCGCTAATgctcgctc        <  3:494018/149‑1 (MQ=255)
                                                                                                                                       gCAAAACATGGC‑CTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAgg                                                >  4:140180/1‑108 (MQ=255)
                                                                                                                                       gCAAAACATGGC‑CTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAgg                                                <  3:140180/108‑1 (MQ=255)
                                                                                                                                         aaaaCATGGCACTAAG‑GCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTCGAGCAGAAAACAGGCTGTAAAAGGACAGTGAATCATGCCCGCTAATGCTCGCTCTCa     >  2:453611/1‑149 (MQ=255)
                                                                                                                                         aaaaCATGGC‑CTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGaa                              >  1:14495/1‑124 (MQ=255)
                                                                                                                                         aaaaCATGGC‑CTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGaa                              <  2:14495/124‑1 (MQ=255)
                                                                                                                                         aaaaCATGGC‑ACTAAGGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGAATCATGCCCGCTAATGCTCGCTCTCa     >  2:595219/1‑149 (MQ=255)
                                                                                                                                          aaaCATGGC‑ACTAAGGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGAATCATGCCCGCTAATGCTCGCTCTCAc    >  1:460164/1‑149 (MQ=255)
                                                                                                                                          aaaCATGGC‑ACTAAGGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGAATCATGCCCGCTAATGCTCGCTCTCAc    >  3:195884/1‑149 (MQ=255)
                                                                                                                                            aCATGGC‑CTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGAATCATGCCCGCTAATGCTCGCTCTCACGc  <  3:324145/149‑1 (MQ=255)
                                                                                                                                            aCATGGC‑ACTAAGGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAgg                                                <  3:170296/103‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                             
CTGGAAGTTGTTCCACTTTATTCTCGACGTCGCCAGCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGC‑CTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGAATCATGCCCGCTAATGCTCGCTCTCACGC  >  NC_000913/3276739‑3277027

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑