BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,074,691	C→G	7.3%	intergenic (+33/‑88)	flu → / → yeeR	CP4‑44 prophage; antigen 43 (Ag43) phase‑variable biofilm formation autotransporter/CP4‑44 prophage; putative membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,074,691	0	C	G	7.3%	386.4 / 17.9	110	intergenic (+33/‑88)	flu/yeeR	CP4‑44 prophage; antigen 43 (Ag43) phase‑variable biofilm formation autotransporter/CP4‑44 prophage; putative membrane protein
Reads supporting (aligned to +/- strand): ref base C (53/49); new base G (3/5); total (56/54)
Fisher's exact test for biased strand distribution p-value = 4.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.91e-01

Only 100 of 122 total aligned reads displayed.

AAGCCCGTGTCCGGGAAAATATCACCCTGGGCGTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCC‑GGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATC‑GCAGGATTTGCCATTCTTCGCCTTT  >  NC_000913/2074543‑2074839

aaGCCCGTGTCCGGGAAAATATCACCCTGGGCGTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCC‑GGtcatc                                                                                                                                                       <  4:201952/149‑1 (MQ=255)

aaGCCCGTGTCCGGGAAAATATCACCCTGGGCGTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCC‑GGtcatc                                                                                                                                                       <  4:309032/149‑1 (MQ=255)

    ccGTGTCCGGGAAAATATCACCCTGGGCGTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCC‑GGTCATCATg                                                                                                                                                    <  1:694873/148‑1 (MQ=255)

    ccGTGTCCGGGAAAATATCACCCTGGGCGTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCC‑GGTCATCATGa                                                                                                                                                   >  4:63975/1‑149 (MQ=255)

     cGTGTCCGGGAAAATATCACCCTGGGCGTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCC‑GGTCATCATGAc                                                                                                                                                  <  4:356355/149‑1 (MQ=255)

        gtCCGGGAAAATATCACCCTGGGCGTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCC‑GGTCATCATGACCg                                                                                                                                                <  1:659371/148‑1 (MQ=255)

        gtCAGGGAAAATATCACCCTGGGCGTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCC‑GGTCATGATGACCgg                                                                                                                                               <  3:952530/149‑1 (MQ=255)

          ccGGGAAAATATCACCCTGGGCGTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCC‑GGTCATGATGACCGGGa                                                                                                                                             <  3:27785/149‑1 (MQ=255)

           cGGGAAAATATCACCCTGGGCGTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCC‑GGTCATGATGACCGGGa                                                                                                                                             <  1:464193/148‑1 (MQ=255)

           cGGGAAAATATCACCCTGGGCGTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCC‑GGTCATCATGACCGGGAc                                                                                                                                            >  4:20087/1‑149 (MQ=255)

           cGGGAAAATATCACCCTGGGCGTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCC‑GGTCATCATGACCGGGAc                                                                                                                                            >  3:917527/1‑149 (MQ=255)

            gggAAAATATCACCCTGGGCGTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCC‑GGTCATCATGAc                                                                                                                                                  <  4:532570/142‑1 (MQ=255)

            gggAAAATATCACCCTGGGCGTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCC‑GGTCATCATGACCGGGAcc                                                                                                                                           >  3:108180/1‑149 (MQ=255)

             ggAAAATATCACCCTGGGCGTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCC‑GGTCATCATGACCGGGAccc                                                                                                                                          <  3:232999/149‑1 (MQ=255)

             ggAAAATATCACCCTGGGCGTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCC‑GGTCATCATGACCGGGAcc                                                                                                                                           >  2:14220/1‑148 (MQ=255)

             ggAAAATATCACCCTGGGCGTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCC‑GGTCATCATGACCGGGAcc                                                                                                                                           >  1:213110/1‑148 (MQ=255)

                aaaTATCACCCTGGGCGTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCC‑GGTCATCATGACCGGGACCCgg                                                                                                                                        <  1:321128/148‑1 (MQ=255)

                     tCACCCTGGGCGTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCC‑GGTCATCATGACCGGGACCCGGACCgg                                                                                                                                   <  2:213110/148‑1 (MQ=255)

                     tCACCCTGGGCGTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCC‑GGTCATCATGACCGGGACCCGGACCgg                                                                                                                                   >  2:466015/1‑148 (MQ=255)

                        cccTGGGCGTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCC‑GGTCATCATGACCGGGACCCGGACCGgcgc                                                                                                                                >  1:295201/1‑148 (MQ=255)

                          cTGGGCGTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCC‑GGTCATCATGACCGGGACCCGGACCGGCGCaa                                                                                                                              >  2:583367/1‑148 (MQ=255)

                             ggCGTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCC‑GGTCATCATGACCGGGACCCGGACCGGCGCAACGGa                                                                                                                          <  3:274738/149‑1 (MQ=255)

                              gCGTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCC‑GGTCATCATGACCGGGACCCGGACCGGCGCAACGGa                                                                                                                          <  2:760146/148‑1 (MQ=255)

                               cGTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCC‑GGTCATCATGACCGGGACCCGGACCGGCGCAACGGAt                                                                                                                         <  1:456239/148‑1 (MQ=255)

                                gTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCC‑GGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCt                                                                                                                       >  3:642745/1‑149 (MQ=255)

                                        cGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCC‑GGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGcc                                                                                                               <  4:376431/149‑1 (MQ=255)

                                                 ccACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCC‑GGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCt                                                                                                      >  4:394573/1‑149 (MQ=255)

                                                  cacaGCGTCAGGGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCC‑GGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTg                                                                                                     >  3:542049/1‑149 (MQ=255)

                                                  cacaGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCC‑GGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTg                                                                                                     <  4:762298/149‑1 (MQ=255)

                                                   acaGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCGCTCTGTGGTCCC‑GGTCATCATGACCGGGACCCGGCCCGGCGCAACGGATCTTCAACGCCACATTCGCTg                                                                                                     <  2:893073/148‑1 (MQ=255)

                                                     aGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCC‑GGTCATCATGACCGGGACCCGGTCCGGCGCAACGGATCTTCAACGCCACATTCGCTGGc                                                                                                   <  1:583367/148‑1 (MQ=255)

                                                          cAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCC‑GGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAAc                                                                                             <  4:75163/149‑1 (MQ=255)

                                                          cAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCC‑GGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAAc                                                                                             <  3:185504/149‑1 (MQ=255)

                                                           aGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCC‑GGTCATCATGACCGGGACCCGGACCGGCGCAACGGAt                                                                                                                         >  3:523046/1‑120 (MQ=255)

                                                           aGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCC‑GGTCATCATGACCGGGACCCGGACCGGCGCAACGGAt                                                                                                                         <  4:523046/120‑1 (MQ=255)

                                                                cagcagCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCC‑GGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATaa                                                                                        <  2:887161/148‑1 (MQ=255)

                                                                     gcgcTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCC‑GGTCATGATGACCgg                                                                                                                                               <  3:518116/88‑1 (MQ=255)

                                                                     gcgcTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCC‑GGTCATGATGACCgg                                                                                                                                               >  4:518116/1‑88 (MQ=255)

                                                                     gcgcTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCC‑GGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGat                                                                                  >  4:410434/1‑149 (MQ=255)

                                                                            aGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCC‑GGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATtcatc                                                                           >  3:256002/1‑149 (MQ=255)

                                                                               gTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCC‑GGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACg                                                                        >  4:921829/1‑149 (MQ=255)

                                                                                 ataACGGTCAGGCCACACTGAATGTGACCTCCTGACAGAACCATCGCCTCTCTGTGGTCCC‑GGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGa                                                                      >  4:874222/1‑149 (MQ=255)

                                                                                  taACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCC‑GGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAg                                                                     >  4:72658/1‑149 (MQ=255)

                                                                                  taACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCC‑GGGCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGAGATTCATCACGGAg                                                                     >  4:56755/1‑149 (MQ=255)

                                                                                   aaCGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCC‑GGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGt                                                                    <  3:833758/149‑1 (MQ=255)

                                                                                     cGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCC‑GGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGa                                                                  <  4:839856/149‑1 (MQ=255)

                                                                                        tCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCC‑GGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACt                                                                >  1:258717/1‑148 (MQ=255)

                                                                                          aGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCC‑GGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATg                                                             <  3:368353/149‑1 (MQ=255)

                                                                                            gCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCC‑GGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGt                                                            <  2:258717/148‑1 (MQ=255)

                                                                                             ccACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCC‑GGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGtt                                                           >  2:574836/1‑148 (MQ=255)

                                                                                               acacTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCC‑GGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTa                                                          >  3:306492/1‑147 (MQ=255)

                                                                                                cacTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCC‑GGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAg                                                       <  4:108180/149‑1 (MQ=255)

                                                                                                  cTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCC‑GGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGa                                                      >  1:520991/1‑148 (MQ=255)

                                                                                                   tGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCC‑GGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATa                                                    >  4:69691/1‑149 (MQ=255)

                                                                                                    gAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCC‑GGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATa                                                    >  1:104323/1‑148 (MQ=255)

                                                                                                     aaTGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCC‑GGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAg                                                   <  1:210711/148‑1 (MQ=255)

                                                                                                       tgtgACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCC‑GGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTc                                                 >  1:826932/1‑148 (MQ=255)

                                                                                                        gtgACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCC‑GGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCg                                                >  2:29299/1‑148 (MQ=255)

                                                                                                        gtgACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCC‑GGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCg                                                <  2:554107/148‑1 (MQ=255)

                                                                                                         tgACCTTCTGACAGAACCCCCGCCTCTCCGTGGTCCC‑GGTCAACATGGACGGGACCCGGAGCGGCGCAACGGATATTCAACGCCACATTCGGGGGAATTTAAAATAACAGAATAATCATCCAGGAAGGACTATGTTACAGATAGTCGgc                                              <  4:335726/149‑1 (MQ=255)

                                                                                                         tgACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCC‑GGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCgg                                               <  1:962190/148‑1 (MQ=255)

                                                                                                           aCCTTCTGACAGAACCATCGCCTCTCTGTGGTCCC‑GGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGgcg                                             >  2:299469/1‑148 (MQ=255)

                                                                                                             cTTCTGACAGAACCATCGCCTCTCTGTGGTCCCTGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGgcgcg                                           >  4:66713/1‑149 (MQ=255)

                                                                                                             cTTCTGACAGAACCATCGCCTCTCTGTGGTCCC‑GGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGgcgcg                                           <  1:910143/148‑1 (MQ=255)

                                                                                                              ttCTGACAGAACCATCGCCTCTCTGTGGTCCC‑GGTCATCATGACCGGGACCCGGAc                                                                                                                                      <  4:408158/56‑1 (MQ=255)

                                                                                                              ttCTGACAGAACCATCGCCTCTCTGTGGTCCC‑GGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGAGTATGTTACAGATAGTCGGCGCGCt                                         >  3:630503/1‑149 (MQ=255)

                                                                                                              ttCTGACAGAACCATCGCCTCTCTGTGGTCCC‑GGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGgcgcgc                                          >  2:190785/1‑148 (MQ=255)

                                                                                                              ttCTGACAGAACCATCGCCTCTCTGTGGTCCC‑GGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCt                                         >  3:371236/1‑149 (MQ=255)

                                                                                                              ttCTGACAGAACCATCGCCTCTCTGTGGTCCC‑GGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCt                                         >  3:868577/1‑149 (MQ=255)

                                                                                                               tCTGACAGAACCATCGCCTCTCTGTGGTCCC‑GGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTg                                        >  4:548852/1‑149 (MQ=255)

                                                                                                                cTGACAGAACCATCGCCTCTCTGTGGTCCC‑GGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGa                                       <  3:410434/149‑1 (MQ=255)

                                                                                                                  gACAGAACCATCGCCTCTCTGTGGTCCC‑GGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATc                                     >  3:340922/1‑149 (MQ=255)

                                                                                                                   aCAGAACCATCGCCTCTCTGTGGTCCC‑GGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATc                                     >  1:70207/1‑148 (MQ=255)

                                                                                                                      gAACCATCGCCTCTCTGTGGTCCC‑GGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCctgc                                 <  4:231297/149‑1 (MQ=255)

                                                                                                                       aaCCATCGCCTCTCTGTGGTCCC‑GGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCctgc                                 <  2:386828/148‑1 (MQ=255)

                                                                                                                          cATCGCCTCTCTGTGGTCCC‑GGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGa                              >  1:521663/1‑148 (MQ=255)

                                                                                                                           aTCGCCTCTCTGTGGTCCC‑GGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTAt                                                              <  1:328335/115‑1 (MQ=255)

                                                                                                                           aTCGCCTCTCTGTGGTCCC‑GGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTAt                                                              >  2:328335/1‑115 (MQ=255)

                                                                                                                                ctctctGTGGTCCC‑GGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATATCATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATC‑GCAgg                      <  3:559898/149‑1 (MQ=255)

                                                                                                                                 tctctGTGGTCCC‑GGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATa                                                    <  3:532509/119‑1 (MQ=255)

                                                                                                                                 tctctGTGGTCCC‑GGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATC‑GCAgg                      >  1:711849/1‑148 (MQ=255)

                                                                                                                                 tctctGTGGTCCC‑GGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGC‑GATCAGCAGGa                     <  3:72658/149‑1 (MQ=255)

                                                                                                                                  ctctGTGGTCCC‑GGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATC‑GCAGGa                     >  2:919591/1‑148 (MQ=255)

                                                                                                                                  ctctGTGGTCCC‑GGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATC‑GCAGGa                     >  2:306399/1‑148 (MQ=255)

                                                                                                                                   tctGTGGTCCC‑GGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATC‑GCAGGAt                    <  1:190785/148‑1 (MQ=255)

                                                                                                                                     tgtgGTCCC‑GGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATC‑GCAGGAttt                  <  1:481734/148‑1 (MQ=255)

                                                                                                                                      gtgGTCCC‑GGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATC‑GCAGGATTTg                 <  1:574836/148‑1 (MQ=255)

                                                                                                                                      gtgGTCCC‑GGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATC‑GCAGGATTTg                 >  1:205153/1‑148 (MQ=255)

                                                                                                                                      gtgGTCCC‑GGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATC‑GCAGGATTTGc                <  3:755004/149‑1 (MQ=255)

                                                                                                                                        ggTCCC‑GGTCATGATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATC‑GCAGGATTTGcc               >  2:247220/1‑148 (MQ=255)

                                                                                                                                           ccc‑GGTCATGATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATC‑GCAGGATTTGCCAtt            >  2:644266/1‑148 (MQ=255)

                                                                                                                                             c‑GGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATC‑GCAGGATTTGCCAttct          >  2:274712/1‑148 (MQ=255)

                                                                                                                                               ggTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATC‑GCAGGATTTGCCAttctt         <  2:104323/148‑1 (MQ=255)

                                                                                                                                               ggTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATC‑GCAGGATTTGCCAttctt         <  1:798681/148‑1 (MQ=255)

                                                                                                                                                 tcatcaTGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATC‑GCAGGATTTGCCATTCTTCg       <  1:299469/148‑1 (MQ=255)

                                                                                                                                                 tcatcaTGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATC‑GCAGGATTTGCCATTCTTCg       <  1:537304/148‑1 (MQ=255)

                                                                                                                                                   atcatGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATC‑GCAGGATTTGCCATTCTTCGcc     <  1:466015/148‑1 (MQ=255)

                                                                                                                                                    tcatGACCGGGACCCGGACCGGCGCAACGGATCTTCAATGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATC‑GAAGGATTTGCCATTCTTCGCCtt   >  4:786470/1‑149 (MQ=255)

                                                                                                                                                     catGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATC‑GCAGGATTTGCCATTCTTCGCCttt  >  3:333675/1‑149 (MQ=255)

                                                                                                                                                     catGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATC‑GCAGGATTTGCCATTCTTCGCCtt   <  2:229718/148‑1 (MQ=255)

AAGCCCGTGTCCGGGAAAATATCACCCTGGGCGTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCC‑GGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATC‑GCAGGATTTGCCATTCTTCGCCTTT  >  NC_000913/2074543‑2074839

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`