BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,116,933	G→T	P97H (CCT→CAT)	glpK ←	glycerol kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,116,933	0	G	T	77.3%	293.1 / 93.9	177	P97H (CCT→CAT)	glpK	glycerol kinase
Reads supporting (aligned to +/- strand): ref base G (20/20); new base T (64/72); total (85/92)
Fisher's exact test for biased strand distribution p-value = 8.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

Only 100 of 180 total aligned reads displayed.

GAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCT‑GGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAA  >  NC_000913/4116660‑4117200

gagGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGCCGGCACTGCCAGACAAAGGCGTTCAAGATATGc                                                                                                                                                                                                                                                                            >  2:611762/1‑276 (MQ=255)

gagGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGAACCACTTCACTCTGGTTCCAGAAAAGTACGGGTCAATCACCAGACCGGTACTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCAATGCCAGACAATGGTGTTATAGATATGc                                                                                                                                                                                                                                                                            >  2:394888/1‑276 (MQ=255)

    cGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGCCAATGGCGTTATAGATATGCTTg                                                                                                                                                                                                                                                                         >  2:619449/1‑275 (MQ=255)

     gTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCCGACCGGTATTGCTGCGGATCTAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTTCGGTACTACGGCACTGCCCGCCCATTGCGTTATAAATAGGCtgcc                                                                                                                                                                                                                                                                        >  2:741683/1‑272 (MQ=255)

          tgtAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATATGCTTGCCGGt                                                                                                                                                                                                                                                                    <  1:31433/274‑1 (MQ=255)

                 ggTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTc                                                                                                                                                                                                                                                           <  4:189261/276‑1 (MQ=255)

                   taacaTGGGCACGGCCCTGAGTCATTTTCCAGATAAGCCACGTAAAAAACGTACCAAACAGCAATGCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCAAGAAAAGTACGGGTCAAGCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATATGCTTGCCGGTTTCTTTTTc                                                                                                                                                                                                                                                           <  3:442846/272‑1 (MQ=255)

                   tCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATATGCTTGCCGGTTTCTTTTTcc                                                                                                                                                                                                                                                          >  3:79791/1‑275 (MQ=255)

                   tCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATATACTTGCCGGTTTCTTTTTccc                                                                                                                                                                                                                                                         >  2:256074/1‑276 (MQ=255)

                        tGGACACGGCCCTGAGTCATTTTCCAAATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGa                                                                                                                                                                                                                                                      <  1:309199/274‑1 (MQ=255)

                        aggACACGGCACACAGTCATTTTCCAGGTAACCCACGTCACAACCGTACCAAACAGCAATTCGCCCCGACGTGCACGCTGGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATATGCTTGCCGGTTTCTTTTTCCCAGa                                                                                                                                                                                                                                                      <  3:561696/273‑1 (MQ=255)

                               ggCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGCTATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATCGGCTTGCCGGTTTTCTTTTCCCAGACAATAGt                                                                                                                                                                                                                                               >  3:402527/1‑274 (MQ=255)

                                 cccTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGTTATAATCTTCTAAACCGTCACGTTTTAAATGCCCGCAGATTTCCTCGGTACGACGGCACTGCCAGACAAAGTCGGTATCGATATCCTTCCCGGTTTCTTTTTCCCACCCACTAgttgtt                                                                                                                                                                                                                                           >  4:348417/1‑276 (MQ=255)

                                  ccataGTCATTTTCCACATATGCCACGTATCAAACCTACCCAACAGCAAATCCCCCCGACGTGCACTATCGCTGGAGCCTTTCACTTTGTCGAGGATCCACTTCACTTTGGGTCCAGATAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAANGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATATGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGttt                                                                                                                                                                                                                                          <  4:437549/272‑1 (MQ=255)

                                     gAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACAGTCACGTTTTAAATGCCCGCAGATTTCTGCGGTCCTACGGCACTGCCAACCAATGGCGTTATAGATATGCTTTCCGTGTTTTTTTTCCCAGACAATAGTGGTTTCa                                                                                                                                                                                                                                        >  3:554598/1‑275 (MQ=255)

                                       gTCATTTTCCATCTATGCACCCTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGATAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATATGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACg                                                                                                                                                                                                                                      <  2:789312/275‑1 (MQ=255)

                                       gTCATTTTCCAGATAATCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCGTCCACATTGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATATGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACg                                                                                                                                                                                                                                      <  1:487569/275‑1 (MQ=255)

                                        acatctcccAGATACTCCACGTATCAACCGTACCCAACAGCAATTCACCACAGCGTGCACGCTCGCGAGAGCCTTCCACCTGGTCGAGGATCCACTTCACTGTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATATGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACg                                                                                                                                                                                                                                      <  4:611762/267‑1 (MQ=255)

                                           ttttCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCCCCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAACGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGAGTTATAGATAGGCTTGCCGGTTTCTTTTTCACAGCCCATAGTGGTTTCAcctctg                                                                                                                                                                                                                                  >  1:301806/1‑270 (MQ=255)

                                           ttttCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTCTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCCCTGCCAGACAATGGCGTTATAGATATGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCT‑gg                                                                                                                                                                                                                                 >  1:404588/1‑275 (MQ=255)

                                             ttCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTAGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTTCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGAGGATATAATCTACTAAACCGTCACGTTTTAAATGATCGCAACTTTCTTCGGTACCACGGCAACGCCAGCCACTGGCGTTATAACTATGCTTGCAGGTTTCTTTTTCCCTGCCAATAGTGGTTTAACgtttctt                                                                                                                                                                                                                                >  4:394761/1‑269 (MQ=255)

                                               ccAGATAAGCCACGTATCAACCGTACCAAACAGCAAGTCACCACGACGTGCACGCTCGCGAGAGCCTTCCGCATGGTCGAGGATCCACTTCACTTTGGGGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATATGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCT‑GGTTCg                                                                                                                                                                                                                             <  1:791481/275‑1 (MQ=255)

                                                caagaaaGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACCTGCACGCTAGCGGGAGCCTTCCACGTGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATATGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCT‑GGTTCGTa                                                                                                                                                                                                                           <  4:589342/271‑1 (MQ=255)

                                                 aGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATATGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCT‑GGTTCGTa                                                                                                                                                                                                                           <  1:568396/275‑1 (MQ=255)

                                                            gTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATATTCTTGCCGGTTTCTTTTTCCCGTACAATAGTGGTTTAACGTC‑GGTTCGTAATACCGATacc                                                                                                                                                                                                                >  3:455629/1‑273 (MQ=255)

                                                                cAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTGCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATATGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCT‑GGTTCGTAATACCGATAGc                                                                                                                                                                                                                <  1:455630/271‑1 (MQ=255)

                                                                      tACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTCTTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCCCGCAGATTCCTGCGTTACGCCGGCCCTGCCACACAATTGCGTTATCGATATGCGTGCCGGTTTCTTTTTCCCAGCCATTAGTGGTTTCCCGCG‑GGTTCGTAGTACCGATAGACGCAATTTGAt                                                                                                                                                                                                     >  4:539169/1‑276 (MQ=255)

                                                                      tACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCT‑GGTTCGTAATACCGATAGCTGCAATTTGa                                                                                                                                                                                                      >  3:653882/1‑275 (MQ=255)

                                                                      tACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGACCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCCCTGCCAGACAATGGCGTTACAGATATGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCCTGCT‑TGTCGGTAATACTGATAGCTGAAAttgca                                                                                                                                                                                                      >  3:328231/1‑272 (MQ=255)

                                                                           aaCAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATATGCTTGCCGGTTTCttt                                                                                                                                                                                                                                                              >  2:70936/1‑215 (MQ=255)

                                                                              aGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAATCGTCACGTTTTAAATGCTCGCAGATTTCTGCTGTACCACCGCACTGCCAGACACTGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATACGGGTTCCCAGTC‑TGTTCTTAGTCGCGATAGCTGTAATTGTATCGGTACt                                                                                                                                                                                              >  4:513214/1‑275 (MQ=255)

                                                                              aGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATATGCTTGCCGGTTTCTTTTTc                                                                                                                                                                                                                                                           <  4:70936/215‑1 (MQ=255)

                                                                                cAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATATGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCT‑GGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTg                                                                                                                                                                                             >  1:333609/1‑274 (MQ=255)

                                                                                cAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGACAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTACACCGTCACGTTTTAAATGCTCGCAGATTTCTTCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATATGCTGGCCGGTTCCTTTTTCCCAACCAATAGTCGTTTCACACT‑GGTTCGTCATACTGATAGCTCCAATTTGATTGGCACTgct                                                                                                                                                                                           >  2:38323/1‑274 (MQ=255)

                                                                                   ttCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATATGCTTGCCGGTTTCTTTTTCACAGACAATAGTGGTTTCACGCT‑GGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGatat                                                                                                                                                                                         >  3:193491/1‑275 (MQ=255)

                                                                                             cgTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGAAGATTTCTGCTGTACGACGGCACTGCCAGACAATGGCGTTATAGATATGCTTGCCGGTTTCTTTTTCCCAGACAAACTTGGTTCTACGCT‑TGTTCGTAATACCGATCACTTCCATTTGATCTGAAATGATATCGGCTTTGGcc                                                                                                                                                                              >  4:336697/1‑276 (MQ=255)

                                                                                             cgTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTGTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGATTCTTTTTCCCAGACCATAGTGGTTTCACGCT‑CGTTTGGACTAACGTGAGTTGCAATTTGATCGGACATGAAATAGGCTTTctc                                                                                                                                                                               >  1:393544/1‑273 (MQ=255)

                                                                                               ggcccGCACGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCCCTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATATGCTTGCCGGTTTCTTTTTCCCGGACAATAGTGGTTTCACGCT‑GGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCa                                                                                                                                                                             <  3:333608/271‑1 (MQ=255)

                                                                                                               ccTTCCGCGTGGTCGAGGATCCACTTCACGTTGGTGCCAGAAAAGGACGAGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTAGAGATATGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCT‑GGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGt                                                                                                                                                             <  4:387858/275‑1 (MQ=255)

                                                                                                               ccTTCCCAATGGTCGAGGATCCACTTCGCTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATATTCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCT‑GGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGt                                                                                                                                                             <  2:22773/275‑1 (MQ=255)

                                                                                                               ccTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATATGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCT‑GGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGt                                                                                                                                                             <  2:126342/275‑1 (MQ=255)

                                                                                                               ccTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATATGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCT‑GGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTg                                                                                                                                                            <  2:288196/276‑1 (MQ=255)

                                                                                                                cTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAACTGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATATCCTTGACGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCCCCAGCACTTCTACCATCggt                                                                                                                                                            >  4:482652/1‑274 (MQ=255)

                                                                                                                cTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATATGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCT‑GGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTg                                                                                                                                                            <  1:79791/275‑1 (MQ=255)

                                                                                                                  tCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGGTATCGATATGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTTACGCT‑GGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGATTTACCCAGCGCTGCTACCAGCGTGGAg                                                                                                                                                         >  2:318026/1‑276 (MQ=255)

                                                                                                                    cacaTGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATATGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCT‑GGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCt                                                                                                                                                       <  2:704268/276‑1 (MQ=255)

                                                                                                                              aGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCT‑GGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGc                                                                                                                                              <  1:521615/275‑1 (MQ=255)

                                                                                                                                     acttcacttTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATATGCTTGCCGGTTTCTTTTTCCCAGACACTAGTGGTTTCACGCT‑GGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGAtt                                                                                                                                       >  3:612457/1‑275 (MQ=255)

                                                                                                                                     acttcacttTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATATGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCT‑GGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGAttt                                                                                                                                      >  2:234293/1‑276 (MQ=255)

                                                                                                                                     acttcacttTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATATGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCT‑GGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGAtt                                                                                                                                       >  1:535923/1‑275 (MQ=255)

                                                                                                                                     acttcacttTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATATGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCT‑GGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACCTCTACTAGCGTGGAGCTTTGGGTGGCCCCGa                                                                                                                                         >  1:108067/1‑273 (MQ=255)

                                                                                                                                     acttcacttTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCT‑GGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGAttt                                                                                                                                      >  2:210697/1‑276 (MQ=255)

                                                                                                                                         cacttTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGTATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATATGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCT‑GGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCCCTTCTCCCAGCGTTGGTCTCTGGGTGGCCCACATTTCCAt                                                                                                                                  >  2:555346/1‑276 (MQ=255)

                                                                                                                                         cacttTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATATGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCT‑GGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCAt                                                                                                                                  >  2:664163/1‑276 (MQ=255)

                                                                                                                                          gcttTGGTGCCCAGAAAGTACGGGTCAGTCACCGGACGGGTATTGCTGCGGTTTTAATCTTCTGAACCGGCACGTTTTAAATGCTCGCAGATTTCTGCGGTCCGACGGCACTGCCAGACAATGGCGTTATAGATATGCTTTCCGGTTTCTTTTTCCCCGACAATAGTGGTTTCCCGCT‑GGTTCGTNATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCAtt                                                                                                                                 <  4:411686/275‑1 (MQ=255)

                                                                                                                                            tttGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTACACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGCCAATGGCGTTCTAGATATGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCT‑GGTTCGTAATACCGATAGCGTCAATCTGCTCTCAACTGGTATGGGCTTTCGCCCGCACTTCTACCAGCGTGGAGCTTTTGGTGGCCCAGCTTTCCAtt                                                                                                                                 >  3:4199/1‑274 (MQ=255)

                                                                                                                                            tttGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATATGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCT‑GGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGAAATCGGTTTTCGCCAGAACTTCTACCAGAGTGGAGCTTTGGGTGGCCCAGTTTTCCATTgg                                                                                                                               >  2:411686/1‑276 (MQ=255)

                                                                                                                                               ggTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCT‑GGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGt                                                                                                                             >  3:154716/1‑275 (MQ=255)

                                                                                                                                                gTGCCAGTAAAGTACGGGTCAATCACCAGCCCGGTATTGCTGCGGATATAATCTTCTAACCCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATATGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCT‑GGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTc                                                                                                                            <  1:328232/275‑1 (MQ=255)

                                                                                                                                                 tGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATATGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCT‑GGTTCGTAATACCGCTTGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCTAGCCCTTCTACCAGCGTGGCGCTTTGGGTGGCCCAGATTTTCCTTGGGt                                                                                                                             >  3:375723/1‑273 (MQ=255)

                                                                                                                                                     aGAAAAGAACGGATCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATGTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATATGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCT‑GGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATATCCATTGGGTCGTGtt                                                                                                                       <  1:111070/275‑1 (MQ=255)

                                                                                                                                                            tACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATATGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCT‑GGTTCGTAATACCGATAGCCGCCATTTGATCGTAACTGATATCGGCTTTCGCCAGCCCTTCTACCAGCGTGGAGCTTTGGGTGGACCAGATTTCCTTTGGGTCGTGTTCTACCCac                                                                                                               >  2:315545/1‑275 (MQ=255)

                                                                                                                                                                ggTCAATCACCAGACCGTTATTGCTGCGGATATAATCTTCTAAGCCGTCACGTTTTAAATGCTCTCAGATTTCTGCGATACGACGGCACTGCCAGACAATGGCGTTATAGATATGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCT‑GGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAAcc                                                                                                             <  1:375724/274‑1 (MQ=255)

                                                                                                                                                                 gTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCATACAATGGCGTTATAGCTATGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCT‑GGTTCGTAATACCGATCGCTGCCATTTGATCGGAATTGATATCGGCTTTCGCCAGCACTTCTCCTATCATCGCGTTTTTGGTGGCCCCGGTTTCCCTTTGGTTGTATTATACCCTAccgcg                                                                                                          >  4:744972/1‑273 (MQ=255)

                                                                                                                                                                 gTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATATGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCT‑GGTTCGTAATACCGATAGCTGCAATTTGATCGGACCTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGATCTTTGTGTGGCCCAGATTTACTTTGGGTCGTGTTCTACCCAACCTgg                                                                                                          >  2:752617/1‑276 (MQ=255)

                                                                                                                                                                                       ctgcGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTTGCAGATTTCTGCGGTACGACTGCACTGCCAGACAATGGCGTTATAGATATGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCT‑GGTTCGTAATACCGTTAGATGCAATTTGATCGGAACTGATATCGGCTTTCGCCCGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGGGTTCTTCCCAACCTGGTTTTGGGTCGATTTGCTCaa                                                                                      >  1:499464/1‑274 (MQ=255)

                                                                                                                                                                                         gcGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATATGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCT‑GGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTTGGTGGCCCAGATTTCCATTGGGTCGTTTTCTACCCACCCTGGTTTTGGGTAGATTTGCTCAAAtt                                                                                   >  2:772269/1‑275 (MQ=255)

                                                                                                                                                                                          cGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACCATAGTGGTTTCACGCT‑GGTTCGTAACACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCCGCACTTCCAACAGCGTGGAGCTTTGGTTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCCACCTGGTTTGTGGTAGATTTGCTCTAAt                                                                                    >  3:259582/1‑273 (MQ=255)

                                                                                                                                                                                            gATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATATGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCT‑GGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTAACCAACCTGGTTTTGGGCAGATTTCCCCTAATTctg                                                                                >  3:288692/1‑273 (MQ=255)

                                                                                                                                                                                             atatGATCTGCCAAACCGTCACGTGTTAAATGGTCGCAGGTTTGTGGGGTACGAGGGCACTGCCAGAGAATGGCGTTATAGAGATGCTTGCCGGTTTCGTTTTCCCAGACAGTAGTGGTTTCACGCT‑GGTTCGTAATACCGATGGCTGCAAGTTGAGCGGAACTGATATCGGCTTTCGCCAGCAGTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATGTGCTCAAATTcgcg                                                                               <  3:369065/275‑1 (MQ=255)

                                                                                                                                                                                               ataATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATATGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCT‑GGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTg                                                                                                                                                            <  2:388951/196‑1 (MQ=255)

                                                                                                                                                                                                  agcttgtAAACCGTGACGGTTTAAATGCTCGCAGATTTGTGCGGTACGACGGCACTGCCAGAAAATGGCGTTATAGATATGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCT‑GGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGa                                                                         <  4:619449/274‑1 (MQ=255)

                                                                                                                                                                                                  aTCTTCTAACCCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATATGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCT‑GGTTCGTAATACCGATAGCTGCAATGTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGa                                                                         <  2:434180/276‑1 (MQ=255)

                                                                                                                                                                                                   tcttctAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATATTCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCT‑GGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGa                                                                         <  3:535922/275‑1 (MQ=255)

                                                                                                                                                                                                     ttctAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATATGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCT‑GGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGaca                                                                       <  4:664163/275‑1 (MQ=255)

                                                                                                                                                                                                      tggataagaggcagTTTTAAATGCTCGCAGATTTCTGCGGTACCAAGTCACTGCCAGACAATGGCGTTATAGATATGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCT‑GGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGacac                                                                      <  4:289483/262‑1 (MQ=255)

                                                                                                                                                                                                      tctAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGTCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCT‑GGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGaca                                                                       <  1:863632/274‑1 (MQ=255)

                                                                                                                                                                                                      tctAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATATGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCT‑GGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGacac                                                                      <  1:554599/275‑1 (MQ=255)

                                                                                                                                                                                                      tctAAACCGTCACGTTTTAAAGGATAGAAAATTTATGCGGGACAAAGGACCTGCCAGACAGTGTCGTTATAGATAGGCTGGCCGTATTCGTTGTCCCAGACAATAGTGGTTTCAAGCT‑GGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCAGCTTTCGCCAGCACTGCTAACAACGTGGAGCTTTGAGTGGCCCAGATTTCCATTGTGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGaca                                                                       <  3:301805/274‑1 (MQ=255)

                                                                                                                                                                                                           aCCGACACTTTTTAAAGGCTCGAGGATTTTTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGGTATGCTTGCCGGTTTCTTTTTCCCAGTCAATAGTGGGTTCACGTT‑GGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGTTATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTa                                                                  <  3:757599/274‑1 (MQ=255)

                                                                                                                                                                                                               tCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATATGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCT‑GGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATTTCGGCTTTCGCCAGCCCTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGGCGTGTTCTACCCAACCTGGTTTGGGGTAGATTTGCTCAATTTCGCGATGCTGAACGGTAATgt                                                              >  1:408022/1‑273 (MQ=255)

                                                                                                                                                                                                               tCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATATGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCT‑GGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGa                                                              >  1:58252/1‑274 (MQ=255)

                                                                                                                                                                                                                      ttAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCT‑GGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCa                                                      <  1:653883/275‑1 (MQ=255)

                                                                                                                                                                                                                         aaTGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATATGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCT‑GGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCg                                                   <  1:123861/275‑1 (MQ=255)

                                                                                                                                                                                                                           tGCTCTAAGATTTCTGCGGGACGACGGCACTGCCAGACAATGGCGTTATAGATATGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCT‑GGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGa                                                <  2:336697/276‑1 (MQ=255)

                                                                                                                                                                                                                            gCTCGGAGATTTTTGCGGTACGACGGCACTGCCAGACAATGGCGTTGTAGATATGCTTGCCGGTTTCTTTTGCCCAGACAATAGTGGTTTCACGCT‑GGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGa                                                <  3:108066/275‑1 (MQ=255)

                                                                                                                                                                                                                            gCTCGCCGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATATGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGa                                                <  2:482652/276‑1 (MQ=255)

                                                                                                                                                                                                                               cGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATATGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACNCT‑GGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTTGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTCGATTTGCCCAAATTCGCGTTGGGACCCGACAATGTTTTTGTcgtcgttaaccc                                            >  4:442800/1‑270 (MQ=255)

                                                                                                                                                                                                                               cGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATATGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCT‑GGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTTGAGCTTTGGGTGCCCCAGTTTTCCATTGGGTCGTGTTCTCCCCAACCTGGTTTTGGGTAGATTTGCCCAAATTCGCGTTGCGACCCGCTACTGATCTTGGCATCGTTCTCCa                                            >  2:271431/1‑276 (MQ=255)

                                                                                                                                                                                                                                       tCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCT‑GGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCCAAAATTCGCGCCTCGACACGCGAATGATATTGGCGTCGTGAACCTTTACGAc                                     >  3:723974/1‑275 (MQ=255)

                                                                                                                                                                                                                                           ccgtccgacgGCCCTCCCAGACAATGGCGTTATCGATATGCTTGCCGGTTTCTTTTTCCCAGAGAATAGTGTGTTCACGCT‑GGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACcgcg                                 <  4:234293/270‑1 (MQ=255)

                                                                                                                                                                                                                                             gtcccacgGCACTGGCAGACAATGGCGTTATTGGTATGCTTGCCGGTTTCTTTTTCCCAGACAAGAGTGGTTTCACGCT‑GGTTCGTAATACCGATAGCTGCAATGTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACcgcgcg                               <  1:751126/270‑1 (MQ=255)

                                                                                                                                                                                                                                                   cgGCACTGCCAAGCCATGGCGTTTTAGGTAGGCTTCCCGGTTTATTTTTCCCAGACAATAGTGGTTTCACGCT‑GGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGTTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGc                          <  3:304895/274‑1 (MQ=255)

                                                                                                                                                                                                                                                     gCACTGCCAGACAATGGCGTTATAGATATGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCT‑GGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCg                                                                          >  4:135318/1‑224 (MQ=255)

                                                                                                                                                                                                                                                      cACGGCCAGACAATGGCGTTATGGATATGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCT‑GGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCAAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCtggtg                     <  2:539169/276‑1 (MQ=255)

                                                                                                                                                                                                                                                        cTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCT‑GGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCtggtgg                    >  1:90234/1‑275 (MQ=255)

                                                                                                                                                                                                                                                                       tttATAGATATGGTGTCCGTTTTCTTTGTCCCAGACAATAGTGGTTTCACGCG‑GGTTCGTAATACCGATAGATGGAATTTGATCGGAGCTGATATCGGCTTTCGCCAGCTCTTCTACCTGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAgcg     <  3:499463/274‑1 (MQ=255)

                                                                                                                                                                                                                                                                         tataGTTATGCCTGCCGGTTTTTTTTTCCCAGACAATAGTGGTTTCACGCT‑GGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGCTATCGGCTCTCGCCAGCACTTCTACCAGCGGGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCa   <  3:155425/275‑1 (MQ=255)

                                                                                                                                                                                                                                                                          ttagatatGCTTGCCGGTTTCTTTTTCCCCGAAAATATGGGTTTCACGCT‑GGTTAGTAATAACGATAGCGGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCaa  <  3:363379/274‑1 (MQ=255)

                                                                                                                                                                                                                                                                          atagatatGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCT‑GGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCa   <  1:852667/274‑1 (MQ=255)

GAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCT‑GGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAA  >  NC_000913/4116660‑4117200

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 9 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`