| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,619,272 | T→C | R27R (CGT→CGC) | rhsB → | Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,619,272 | 0 | T | C | 98.5% | 199.6 / ‑6.5 | 68 | R27R (CGT→CGC) | rhsB | Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (41/25); total (43/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01 | |||||||||||
TGGGTCCTTTGGTGAACGATCAACAAAGGGCCACTAGCACACCTGATTCCTCTCTAAATACCCTATCCGAACCTTCTCTTTTGTAACGTTCTAAATATATTCCTAAAAATCTTCAATTCATTGTGACCACAAGTTTTTCTTCGCTTTTTCGTATGAAGAT‑ACTGTCATTAAAATAATAGAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGTGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTACGTATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATCCTCTCCCGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTGGGG > NC_000913/3618999‑3619461 | tGGTTCCTTTGGTGAACGATCAACAAAGGGCCACTAGATCACCTGATTACTCTTTAAATACCCTATCCGAACCTTATCTTTTGTAACGTTCTAAATATATTCCTAAAAATCTTCAATTCATTGTGACCACAAGTTTTTCTTCGCTTTTTCGTATGAAGAT‑ACTGTCATTAAAATAATAGAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGTGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCATTg < 4:634133/278‑1 (MQ=255)tGGGTCCGTGTGTGAAACATAAACAAAGGGCCACTAGCACACCTGATTCCTCTCTAAATCCCCTATCCGAAACTTCGCTTTTGTAACGTTCTAAATATATTCCTAAAAATCTTCAATTCATTGTGACCACAAGTTTTTCTTCGCTTTTTCGAATGAAGAT‑ACTGTCATTAAAATAATAGAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGTGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCATTg < 4:410203/278‑1 (MQ=38) gTCCTTTGGTGAACGATCAACAAAGTGCCACTAGCACACCTGATTCCTCTCTAAATACCCTATCCGAACCTTCACTTTTGTAACGTTCTAAATATATTCCTAAAAATCTTCAATTCATTGTGACCACAAGTTTTTCTTCGCTTTTTCGTATGAAGAT‑ACTGTCATTAAAATAATAGAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGTGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCATTg < 1:683600/275‑1 (MQ=255) cTTTGGTGAACGATCAACAAAGGGCCACTAGCACACCTGATTCCTCTCTAAATACCCTAACCGAACCTTCTCTTTTGTAACGTTCTAAATATATTCCTAAAAATCTTCAATTCATTGTGACCACAAGTTTTTCTTCGCTTTTTCGTATGAAGAT‑ACTTTCATTAAAATAATAGAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGTGTCAGGGTGACATGACGCAGTATGGCGGAAGCATTGTTCAGGGTTCAGTCGGGGTGCGCATTGGTg > 1:459457/1‑275 (MQ=255) ttGGGGAACGAACAACAAAGGGCCAATAGCACAACTGATTCCTCTCTAAATACCCATTCCGAACCTTCTCTTTTGTAACGTTCTAAATATATTCCTAAAAATCTTCAATTCATTGTGACCACAAGTTTTTCTTCGCTTTTTCGTATGAAGAT‑ACTGTCATTAAAATAATAGAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGTGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCATTGGTGcc < 3:332151/275‑1 (MQ=255) tGAACGATCAACAAAGGGCCAATAGCACAGCAGATTCCTCTCTCAATACCCTATCCGAACCGTCTCTTTTGGAACGTTCTAAAGATAATCCTAAAAATCTTCAATTCATTGTGACCACAAGTTTTTCTTCGCTTTTTCGTATGAAGAT‑ACTGTCATTAAAATAATAGAAAAGGATTTTACGATGAGCCGAAAATCGGCAGCGCGTCAGGGTGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCATTGGTGCCCCCa < 3:459456/275‑1 (MQ=38) aaCGATCAACAAAGGGCCACTAGCACACCTGATTCCTCTCTAAATACCCTATCCGAACCTTCTCTTTTGTAACGTTCTAAATATATTCCTAAAAATCTTCAATTCATTGTGACCACAAGTTTTTCTTCGCTTTTTCGTATGAAGAT‑ACTGTCATTAAAATAATAGAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGTGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTTCGTATTGGGGCCTCCACCGGc > 2:582155/1‑278 (MQ=255) aaCGATCAACAAAGGGCCACTAGCACACCTGATTCCTCTCTAAATACCCTATCCGAACCTTCTCTTTTGTAACGTTCTAAATATATTCCTAAAAATCTTCAATTCATTGTGACCACAAGTTTTTCTTCGCTTTTTCGTATGAAGAT‑ACTGTCATTAAAATAATAGAAAAGAATTTTACGATGAGCGCAAAACCGGCGGCGCGCCAGGGTGACATGACGCATTTTGGCGTTAGACTTGTTAAGGTTTCACCCGGGTTGCGCATTGGTtcctcc > 1:426949/1‑272 (MQ=255) ataaacaaAGGGCCACTTGAACACCTGATTACTCGCTAACTACCCTATCCGAACCTTCTCTTTTGTAACGTTCTAAATATATTCCTAAAAATCTTCAAATCAATGTGACCACAAGTTTTTCTTCGCTTTTTCGTATGAAGAT‑ACTGTCATTAAAATAATAGAAAAGGATGTTACGATGGGCGGAAAACCGGCGGCGCGTCAGGGTGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCATTGGTGCCCCCACCGGc < 3:678741/271‑1 (MQ=37) aTCAACAAAGGGCCACTAGCACACCTGATTCCTCTCTAAATACCCTATCCGAACCTTCTCTTTTGTAACGTTCTAAATATATTCCTAAAAATCTTCAATTCATTGTGACCACAAGTTTTTCTTCGCTTTTTCGTATGAAGAT‑ACTGTCATTAAAATAATAGAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGTGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCATTGGTGCCCCCACCGGc < 3:546681/274‑1 (MQ=255) aTCAACAAAGGGCCACTAGCACACCTGATTCCTCTCTAAATACCCTATCCGAACCTTCTCTTTTGTAACGTTCTAAATATATTCCTAAAAATCTTCAATTCATTGTGACCACAAGTTTTTCTTCGCTTTTTCGTATGAAGAT‑ACTGTCATTAAAATAATAGAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGTGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCATTGGTGCCCCCACCGGc < 1:588548/274‑1 (MQ=255) cTAGCACACCTGATTCCTCTCTAAATACCCTATCCGAACCTTCTCTTTTGTAACGTTCTAAATATATTCCTAAAAATCTTCAATTCATTGTGACCACAAGTTTTTCTTCGCTTTTTCGTATGAAGAT‑ACTGTCATTAAAATAATAGAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGTGACATGACGCAGTATGGCGGTAGCATTGTTTAGGGTTCCGCCGGGGTGCGCATTGGTGCCCCCACCGCCGTGGCCTGTTGTGTGTGcg > 4:224187/1‑277 (MQ=255) cTAGCACACCTGATTCCTCTCTAAATACCCTATCCGAACCTTCTCTTTTGTAACGTTCTAAATATATTCCTAAAAATCTTCAATTCATTGTGACCACAAGTTTTTCTTCGCTTTTTCGTATGAAGAT‑ACTGTCATTAAAATAATAGAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGTGACATGACGCAGTATGGCGGTAGCAGTGTTCAGGGTTCAGCCGGGGTGCGCATTGGTGCCCCCACCGGCGTGGCCTGCTCGGTGTGcc > 2:218604/1‑278 (MQ=255) gCACACCTGATTCCTTTCTAAATACCCTATCCGAACCTTCTCTTTTGTAACGTTCTAAATATATTCCTAAAACTCTTCAATTCATTGTGACCACAAGTTTTTCTTCGCTTTTTCGTATGAAGAT‑ACTGTCATTAAAATAATAGAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCACTCGGGGTGCGCACTGGTGCCCCCCCCGGCGTGGCCTGTTCGGTGTGc > 1:235818/1‑274 (MQ=255) acacCTGATTCCTCTCTAAATACCCTATCCGAACCTTCTCTTTTGTAACGTTCTAAATATATTCCTAAAAATCTTCAATTCATTGTGACCACAAGTTTTTCTTCGCTTTTTCGTATGAAGAT‑ACTGTCATTAAAATAATAGAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGTGACATGACGCAGTATGGCGGTAGCATGGTTCAGGGTTCAGCCGGGGTGCGCATTGGTGCCGCCACCGCCGTGGCTGGTTCGGTGTGcc > 4:429570/1‑273 (MQ=255) cacCTGATTCCTCTCTAAATACCCTATCCGAACCTTCTCTTTTGTAACGTTCTAAATATATTCCTAAAAATCTTCAATTCATTGTGACCACAAGTTTTTCTTCGCTTTTTCGTATGAAGAT‑ACTGTCATTAAAATAATAGAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGTGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCcggc > 4:434333/1‑277 (MQ=255) cTGATTCCTCTCTAAATCCACTATCCGCACCTTCTCTTTTGTAACGTTCTAAATATATTCCTAAAAATCTTCAATTCATTGTGACCACAAGTTTTTCTTCGCTTTTTCGGATGAAGAT‑ACTGTCATTAAAATAATAGAAAAGGATTTTACGATGAGCGTAAAACCTGCGGCGCGTCAGGGTTACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAg < 2:137526/278‑1 (MQ=255) tGATTCCTCTCTAAATACCCTATCCGAACCTTCTCTTTTGTAACGTTCTAAATATATTCCTAAAAATCTTCAATTCATTGTGACCACAAGTTTTTCTTCGCTTTTTCGTATGAAGAT‑ACTGTCATTAAAATAATAGAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGTGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGt < 2:274931/278‑1 (MQ=255) gATTCCTCTCTAAATACCCTATCCGAACCTTCTCTTTTGTAACGTTCTAAATATATTCCTAAAAATCTTCAATTCATTGTGACCACAAGTTTTTCTTCGCTTTTTCGTATGAAGAT‑ACTGTCATTAAAATAATAGAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGTGACATGACGCCGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGTGTGCGCATTGGTGCCCCCCCCGGCGTGTCCTGGTCGGTGTGCCTCGGCGGAGt > 4:502041/1‑277 (MQ=255) tCCTCTCTAAATACCCTATCCGAACCTTCTCTTTTGTAACGTTCTAAATATATTCCTAAAAATCTTCAATTCATTGTGACCACAAGTTTTTCTTCGCTTTTTCGTATGAAGAT‑ACTGTCATTAAAATAATAGAAAAGGATTTTACGATGATCGGAAAACCGGCGGCGCGTCAGGGTGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCATTGGTGCACCCACCGGCGTGGCCAGTTCGGTGTCCCCCTGCGGAGTGCCg > 2:418178/1‑278 (MQ=255) tctctAAATACCCTATCCGAACCTTCTCTTTTGTAACGTTCTAAATATATTCCTAAAAATCTTCAATTCATTGTGACCACAAGTTTTTCTTCGCTTTTTCGTATGAAGAT‑ACTGTCATTAAAATAATAGAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGTGACATGACGCAGTATGGCGGTAGCTTTGTTCAGGGTTCAGCCGGGGTGCGCATTGTTTCCCCCACCGGCTGGGCCTGTTCGGTGGCCCCcggcgggtggac > 3:703514/1‑268 (MQ=255) tctctAAATACCCTATCCGAACCTTCTCTTTTGTAACGTTCTAAATATATTCCTAAAAATCTTCAATTCATTGTGACCACAAGTTTTTCTTCGCTTTTTCGTATGAAGAT‑ACTGTCATTAAAATAATAGAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGTGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCATTGGTGcc > 1:191563/1‑233 (MQ=255) tctAAATACCCTATCCGAACCTTCTCTTTTGTAACGTTCTAAATATATTCCTAAAAATCTTCAATTCATTGTGACCACAAGTTTTTCTTCGCTTTTTCGTATGAAGAT‑ACTGTCATTAAAATAATAGAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGTGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTc > 1:165867/1‑275 (MQ=255) tctAAATACCCTATCCGAACCTTCTCTTTTGTAACGTTCTAAATATATTCCTAAAAATCTTCAATTCATTGTGACCACAAGTTTTTCTTCGCTTTTTCGTATGAAGAT‑ACTGTCATTAAAATAATAGAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGTGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTc > 3:336989/1‑275 (MQ=255) tAAATACCCTATCCGAACCTTCTCTTTTGTAACGTTCTAAATATATTCCTAAAAATCTTCAATTCATTGTGACCACAAGTTTTTCTTCGCTTTTTCGTATGAAGAT‑ACTGTCATTAAAATAATAGAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGTGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCATTGGTGCCCCCa < 3:191562/233‑1 (MQ=255) tACCCTATCCGAACCTTCTCTTTTGTAACGTTCTAAATGTATTCCTAAAAATCTTCAATTCATTGTGACCACAAGTTTTTCTTCGCTTTTTCGTATGAAGAT‑ACTGTCATTAAAATAATAGAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGTGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCa < 1:31936/275‑1 (MQ=255) atcccTATCCGAACCTTCACGTTTTTAACGTTCTAGATATTTTCATAAACATCATCAATTCATTGTGACCACAAGTTTTTCTTGGCTTTTTCGTATGAAGAT‑ACTGTCATTAAAATAATAGAAAAGGATTATACGATGAGCGGAAAACCGGCGGCGCGTCAGGGTGACATGACGCAGTATGGCGGTANCATGGTTCAGGGTTCAACCGGGGTGCGCATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATcc < 4:352936/276‑1 (MQ=21) tctccgaggcatctcTATTGTAACGTTCTCAATCTAGTCCTGAAAAACTTCAATTCATTGTGACCACAAGTTTTTCTACGATTTTTCGTATGAAGAT‑ACTGTCATTAAAATAATAGAAAAGGTTTTTACGATGAGCGGAAAACCGGAGGCGCGTCAGGGTGACATGACGCAGTATGGCGGTAGCATTNTTCAGGGTTCAGCCGGGGTGCGCATTGGTGCCCCCACCGGCTTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCa < 4:354814/267‑1 (MQ=21) acccGAACCTTCTCTTTTGTAACGTTCTAAATATATTCCTAAAAATCTTCAATTCATTGTGACCACCAGTTTTTCTTCGCTTTTTCGTCTGAAGAT‑ACTGTCATCAAAATAATAGAAAAGGATTTTACGATGAGCGGACAACCGGCGGCGCGTCCGGGTGACATGACGCAGTATGGCGGTAGCAGTGTTCAGGGTTCACGCGGGGTGCGAATTGGTGCCCGCACGGGGGTGGCCTGTTCGGGGTGACCCGGGGGTTTGTCGTCCGGCCCTcccgg > 3:453679/3‑272 (MQ=255) aTCCGAACCTTCTCTTTTGTAACGTTCTAAATATATTCCTAAAAATCTTCAATTCATTGTGACCACAAGTTTTTCTTCGCTTTTTCGTATGAAGAT‑ACTGTCATTAAAATAATAGAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGTGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGt > 1:727709/1‑275 (MQ=255) aTCCGAACCTTCTCTTTTGTAACGTTCTAAATATATTCCTAAAAATCTTCAATTCATTGTGACCACAAGTTTTTCTTCGCTTTTTCGTATGAAGAT‑ACTGTCATTAAAATAATAGAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGTGACATGACGCAGCATGGCGGTAGCATTGTTCAGGGTTCACGCGGGGGGCGCATTGGTTGCCCCAGCGGCGTGGCCGGTCCGGTGCTCCGCGGCGGGGTGGCGGCCGGCCATCAGGt > 3:111629/1‑275 (MQ=255) ccTTCTCTTTTGTAACGTTCTAAATATATTCCTAAAAATCTTCAATTCATTGTGACCACAAGTTTTTCTTCGCTTTTTCGTATGAAGAT‑ACTGTCATTAAAATAATAGAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGTGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATccc > 1:505307/1‑275 (MQ=255) ctctTTTGTAACGTTCTAAATATATTCCTAAAAATCTTCAATTCATTGTGACCACAAGTTTTTCTTCGCTTTTTCGTATGAAGAT‑CCTGTCATTAAAATAATAGAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGTGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTTCGCATTGGTGCCCCCCCCGGCGTGGCCTGTTCGGTTTGCCCCGGGGGCGTGACGTCCGCCCCTCCGGTCACTCCCTTg > 3:331569/1‑274 (MQ=25) tctTTTGTAACGTTCTAAATATATTCCTTAACATATTCAATTCCTTTTGACACCAAGTGTTTCTTCGTTTTTTCGTATGAAGAT‑ACTTTCATTAAAATAATAGAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGTGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGTTGCGCATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAACCCCCTGCt < 3:202263/275‑1 (MQ=21) ctTTTGTAACGTTCTAAATATATTCCTAAAAATCTTCAATTCATTGTGACCACAAGTTTTTCTTCGCTTTTTCGTATGAAGAT‑ACTGTCATTAAAATAATAGAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGTGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTc < 1:336990/275‑1 (MQ=255) ttgtGTAACGTTCTAAATATTTTGCTAAAAATCTTCAATTCATGTGGACCTCAAGTTTTTCTTCGCTTGTTCGTATGAATAT‑ACTGTCATTAAAAGAATAGAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGGCAGGGTGACAGGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCg < 1:111630/272‑1 (MQ=18) tGTAACGTTCTAAATATATTCCTAAAAATCTTCAATTCATTGTGACCACAAGTTTTTCTTCGCTTTTTCGTATGAAGAT‑ACTGTCATTAAAATAATAGAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGTGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACCTCGGGCCACACGGTCAAACCCCTGCTCGGTg > 1:528976/1‑275 (MQ=255) ttCTAAATATATTCCTAAAAATCTTCAATTCATTGTGACCACAAGTTTTTCTTCGCTTTTTCGTATGAAGAT‑ACTGTCATTAAAATAATAGAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGTGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAgt > 3:368164/1‑273 (MQ=255) ttCTAAATATATTCCTAAAAATCTTCAATTCATTGTGACCACAAGTTTTTCTTCGCTTTTTCGTATGAAGAT‑ACTGTCATTAAAATAATAGAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGTGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGGGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAgg > 1:584047/1‑274 (MQ=255) tCTAAATATATTCCTAAAAATCTTCAATTCATTGTGACCACAAGTTTTTCTTCGCTTTTTCGTATGAAGAT‑ACTGTCATTAAAATAATAGAAAAGGATTTTACGATGGGCGGAAAGCCGGCGGCGCGTCAGGGTGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATcc < 2:111255/247‑1 (MQ=255) atTCCTAAAAATCTTCAATTCATTGTGACCACAAGTTTTTCTTCGCTTTTTCGTATGAAGAT‑ACTGTCATTAAAATAATAGAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGTGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAGGTCCTTCCCGGTGa > 2:408331/1‑277 (MQ=255) atTCCTAAAAATCTTCAATTCATTGTGACCACAAGTTTTTCTTCGCTTTTTCGTATGAAGAT‑ACTGTCATTAAAATAATAGAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGTGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAGGGCCTTCCCGGTGa > 2:631802/1‑277 (MQ=255) atTCCTAAAAATCTTCAATTCATTGTGACCACAAGTTTTTCTTCGCTTTTTCGTATGAAGAT‑ACTGTCATTAAAATAATAGAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGTGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCCCGGTGCAAAGGTCCTTCCCgg > 3:310209/1‑274 (MQ=255) ccTAAAAATCTTCAATTCATTGTGACCACAAGTTTTTCTTCGCTTTTTCGTATGAAGAT‑ACTGTCATTAAAATAATAGACAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGTGACATTACGCAGTATGGCGGTAGCATCGTTCAGGGTTCAGCCGGGGGCCGCACTGGTGCCCCTACCGGCGTGTCCTGGTGGGTGTTCCCCGCCGGTGTGCCGTCCGGCCATTCGGTCACCCCCCTGCCGGGTGCAACGGTCCTTCCCGGt > 3:56910/1‑272 (MQ=255) aaaaTCTTCAATTCATTGTGACCACAAGTTTTTCTTCGCTTTTTCGTATGAAGAT‑ACTGTCATTAAAATAATAGAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGTGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCCCGGGGCAAAGGGCCTTCCCGGTGAAACCGAc > 4:423452/1‑277 (MQ=255) tCAATTCATTGTGACCACAAGTTTTTCTTCGCTTTTTCGTATGAAGAT‑ACTGTCATTAAAATAATAGAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGTGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAGGTCCTTCCCGGTGAACCCGACATCGccc > 2:177619/1‑277 (MQ=255) aTTGTGACCACAAGTTTTTCTTCGCTTTTTCGTATGAAGAT‑ACTGTCATTAAAATAATAGAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGTGACATGACGCAGTATGGCGGTAGCATTGTTCGGGGTTCAGCCGGGGTGCGCATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTTTTCCCCGGCGGAGTGACGTCCGGCCACCCGGTCCATCCCCCGCCCGGTGCAAAGGTCCTTCCCGGCGAACCCGACATCGCCCTgcc > 1:460762/1‑274 (MQ=18) gACCACAAGTTTTTCTTCGCTTTTTCGTATGAAGAT‑ACTGTCATTAAAATAATAGAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGTGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAGGTCCTTCCCGGTGAACCCGACATCGCCCTtccctgcc > 3:57832/1‑274 (MQ=255) gACCACAAGTTTTTCTTCGCTTTTTCGTATGAAGAT‑ACTGTCATTAAAATAATAGAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGTGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAGGTCCTTCATGGTGAAACCGACAACGCCCTgcccggc > 1:118997/1‑273 (MQ=255) aCCACAAGTTTTTGTTCTCTGTTTCGTCAG‑AGATGAATGGCATTAAAAAAATAGAAAAGGATTGCACGATGGGCGGAAAACCGGCGGCGCGTCAGGGTGACAGTACGCAGTGTGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCATAGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTg < 4:408331/278‑1 (MQ=14) caAGTTTTTCTTCGCTTTTTCGTATGAAGAT‑ACTGTCATTAAAATAATAGAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGTGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTTCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCg > 2:194235/1‑277 (MQ=255) aaGTTTTTCTTCGCTTTTTCGTATGAAGAT‑ACTGTCATTAAAATAATAGAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGTGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCATTCCCCGGCCCGGTTCAAAGGGCCTTCCCGGTCAAACCGACATCGCCCTGCCCGGCCCGCTGc > 3:435656/1‑274 (MQ=255) tttCTTCGCTTTTTCGTATGAAGAT‑ACTGTCATGAAAATAATAGAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGTGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATc < 2:429570/277‑1 (MQ=255) tcGCTTTTTCGTATGAAGAT‑ACTGTCATTAAAATAATAGAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGTGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCAGTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCCTCCGGTCAATCTCCTGCTCGGTGCCAAGGTCCTTGCCGGTGAAACCGACATCGCCCTTCCCGGTCCCCTGCCGTTctcccc > 3:235411/1‑269 (MQ=17) ttttCGTATGAAGAT‑ACTGTCATTAAAATAATAGAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGTGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATCCTCTCCCGc < 2:434333/276‑1 (MQ=255) ttttCGTATGAAGAG‑ACGGTCATTAAAATAATAGAAAAGGATTTTACGAGGAGCGGAAAACCGGCGGCGCGTCAGGGTGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATCCTCTCCCGc < 2:502041/276‑1 (MQ=255) tCGTATGAAGAT‑ACTGTCATTAAAATAATAGAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGTGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATCCTCTCCCGc < 3:727708/273‑1 (MQ=255) tCGTAAGAAGAT‑AATGTCATTAAAATAATAGAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGTGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGGGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATCCTCTCCCGc < 1:301235/273‑1 (MQ=255) cTGTCATTAACATAATAGAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGTGACATGACGCAGTATGGCGGTAGCATTGNTCAGGGTTCAGCCGGGGTGCGCATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGCCGTCCGGCCACCCGGTCAATCCACTGCTCGGTGCAAAGGGCCTTCCCGGTGAAACCGTCATCGCCCGGCCCGGCCCTCTGCCAGTCACCCTCGCCCGCACCTAccgcatgcaccg > 4:331233/1‑270 (MQ=14) cATTAAAATAATAGAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGTGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCATTGGTGCCCTCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCACAGGTCCTTCCCGGTGAAACCGACATCGCCCTCCCCGGCCCGCTGCCGTTCACCCCCTCCCGCACCTACAGCATTTCCCGGACa > 2:188259/1‑277 (MQ=255) cATTAAAATAATAGAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGTGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGCCAATCCCCTGCTCGGTGCCAAGGCCCTTCCCGGTGAAACCGACATCGCCCTGCCCTGTCCGCTGCCGTTCATCCTCTCCCGCCTCTACACCACTTccctcacc > 4:64305/1‑272 (MQ=14) aTTAAAATAATAGAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGTGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACCTCCCCCTTCCCGGCCCGCTGCCGTTCACCCCCTCCCGCACCTACAGCCTTTACCGGa > 3:298370/1‑274 (MQ=255) ataGGAAAGGGCTTTACGATGAGCGGAAAGCCAGCGGCGCGTCAGGGGGACATGGCGCAGTATGGCGGTAGCAGTGTTCAGGGTTCAGCCGGGGTGCGCATTGGTGCCCCCACCGGCCTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAAGCCCCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATCCTCTCCCGCACCTACAGCAGTTACCGGACAAAAACGcc < 3:234409/275‑1 (MQ=11) taGAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGTGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATCCTCTCCCGCACCTACAGCAGTTACCGGACAAAAACGCCcgc > 2:180806/1‑277 (MQ=37) taGAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGTGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCAACCGGTCAATCCCCTGCTCGGTGCAAAGGTCCTTCCCGGTGACACCGACCTCGCACTGCCCGGCCCGCTGCCGTTCATCCTCTCCCCCCCCTACCGCAGTTACCCGACAAAACCGcc > 3:413052/1‑274 (MQ=11) tttACGATGAGCGGAAAACCGGCGGCGCGTCAGGGTGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATCCCCTCCCGCACCTACAGCAGTTACCGGCCAAAAACGCCCGCGCCGGTggg > 1:226918/1‑275 (MQ=17) tACGATGAGCGGAAAACCGGCGGCGCGTCAGGGTGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCCCTGCCGTCCATCCTCTCCCGCCCCTTCAGCAGTTACCGGCCAAAAACGCCCGCGCCGGTgggg > 1:116129/1‑274 (MQ=9) tACGATGAGCGGAAAACCGGCGGCGCGTCAGGGTGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCACCGGCGGTGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCCTCCCCTCCCGCACCTACAGCAGTTACCGGACAAAACCCCCCGCGCCGGTgggg > 1:709516/1‑274 (MQ=255) | TGGGTCCTTTGGTGAACGATCAACAAAGGGCCACTAGCACACCTGATTCCTCTCTAAATACCCTATCCGAACCTTCTCTTTTGTAACGTTCTAAATATATTCCTAAAAATCTTCAATTCATTGTGACCACAAGTTTTTCTTCGCTTTTTCGTATGAAGAT‑ACTGTCATTAAAATAATAGAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGTGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTACGTATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATCCTCTCCCGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTGGGG > NC_000913/3618999‑3619461 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |