BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,116,538	T→G	N229H (AAC→CAC)	glpK ←	glycerol kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,116,538	0	T	G	98.1%	556.7 / NA	161	N229H (AAC→CAC)	glpK	glycerol kinase
Reads supporting (aligned to +/- strand): ref base T (2/1); new base G (69/87); total (71/90)
Fisher's exact test for biased strand distribution p-value = 5.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 161 total aligned reads displayed.

CATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTT‑CTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCAT‑AAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCA‑TTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACT  >  NC_000913/4116265‑4116795

cctCGCCCCGCCACTGAATGGATGCGCCTGCCATAAACACCGCCCCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTT‑CTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCAT‑AAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTc                                                                                                                                                                                                                                                                <  2:331715/276‑1 (MQ=255)

catCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTT‑CTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCAT‑AAAGCAGCCAGTGCCATAGGTGTTCTACGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTc                                                                                                                                                                                                                                                                <  2:415689/278‑1 (MQ=255)

   cgggcAGCCACTGAATGGGTGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTT‑CTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCAT‑AAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTc                                                                                                                                                                                                                                                                <  1:32982/272‑1 (MQ=255)

   cgcgCAGCGCCTGAATGGATGCGCCTGCCGTAAACACCGCACCTTCCAACGTATAGTTCACGTCGCCACTCGGGCCGAAGGCGATGGTGGTCAGCAGGCCGTTTT‑CTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCAT‑AAATCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTc                                                                                                                                                                                                                                                                <  1:288297/275‑1 (MQ=255)

   cgcgCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTT‑CTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCAT‑AAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTc                                                                                                                                                                                                                                                                <  1:160354/275‑1 (MQ=255)

        cgCCACGGAATGGCTGCGCCTGCCATCAACCCCGCACCGTCCGACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTT‑CTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCAT‑AAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGta                                                                                                                                                                                                                                                        <  4:530442/277‑1 (MQ=255)

        aGCCACTGAATGTATGCGCCTGCCATAACCACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTT‑CTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCAT‑AAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGta                                                                                                                                                                                                                                                        <  2:465039/278‑1 (MQ=255)

           ctcTGATTGCATGCGCCTGTCATCAACTCCGCTCCGTCCATGCGAGAGTGCGCTTGTCCAGTCGTGCCGCATGCGATGGTTGTCAGCAGGCCATTTT‑CTGATGTGACCGCTTTCTCGCCAGTGTGCATCAGCAT‑AAAGCAGCCAGGGCCATAGGTGTTCGTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGGGATTGAAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGta                                                                                                                                                                                                                                                        <  3:111661/273‑1 (MQ=255)

           cACTGAATGGGTGCGCCGGCCATAAACACCGCACCTTCCGACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGAGGGTGGTCAGCAGGCCGTTTT‑CTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCAT‑AAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCGTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGta                                                                                                                                                                                                                                                        <  1:605140/275‑1 (MQ=255)

           cACTGAATGGATGCGCCTGCCATCAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTT‑CTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCAT‑AAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGta                                                                                                                                                                                                                                                        <  1:193648/275‑1 (MQ=255)

                aaTGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTT‑CTGATTTCACCGCTTTCTTGCCAGTGTTCATCAGCAT‑AAAGCAGACAGTGCCATAGGTGTTCTTCTCCTTCCCTTCTTTCACGCACAACTGACCAAACAGCGCGTCCTGCTGGTCACCGGCGATCCCGGAGATTGGCATACCAGTGCCGCCTTTGCCCCCAATGTGAGTCTGACCGTCTACt                                                                                                                                                                                                                                                    >  1:223268/1‑274 (MQ=255)

                  tGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTT‑CTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCAT‑AAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCTTGCTGGTCACCGGCGATCCCGGCGATTGGAATACGCGTTCCGCCTTTGCCCCCACTGTTAGTCTCACCGTATCCTTccaa                                                                                                                                                                                                                                               >  4:315895/1‑274 (MQ=255)

                          ccTGCCATAAACACCGCACTTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTT‑CTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCAT‑AAAGCAGCCAGTGCCATAGGTGTGCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTAACCGGCGACCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAGGAacaagg                                                                                                                                                                                                                                      >  2:195351/1‑274 (MQ=255)

                          ccTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGACGCAGGCGATGGTGGTCAGCAGGCCGTTTT‑CTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCAT‑AAAGCAGCCAGTGCCATAGGTGTTATTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGACTGCTGGGCACCGGCGATCCCGGAGATTGGAATCCGCGTGCCGCCTTTGCCGCCAATGTGCAGCTGACCGTATACTTCGGAAGAacgacc                                                                                                                                                                                                                                      >  2:463068/1‑277 (MQ=255)

                                        cGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTT‑CTGATTTCCCCGCTTTCTCGCCAGTGTTCATCAGCAT‑AAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCGCGTCACCGGCGATCCAGGATATCGGAATACGCGTGCCGCCTCTCCCGCCAATGTGTGTCTTGCCGTTTACTTCGGAAGAACGACGccattttgtcc                                                                                                                                                                                                                           >  3:338511/1‑265 (MQ=255)

                                        cGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTT‑CTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCAT‑AAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGACCGACGCACTTCTGgca                                                                                                                                                                                                                           >  3:723993/1‑275 (MQ=255)

                                        cGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTT‑CTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCAT‑AAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGgca                                                                                                                                                                                                                           >  3:607394/1‑275 (MQ=255)

                                        cGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTT‑CTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCAT‑AAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGgca                                                                                                                                                                                                                           >  3:289915/1‑275 (MQ=255)

                                        cGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTT‑CTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCAT‑AAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTAAACTTCGGCAGAACGACGCACTTCTGgca                                                                                                                                                                                                                           >  1:109046/1‑275 (MQ=255)

                                         gCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTT‑CTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCAT‑AAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGCTTGGAATACGCGTGCCGCCTTTGCCGCCACTGGTAGTCTGCCCGTATACTTCGGAAGAACCACGCACTTCTGgcac                                                                                                                                                                                                                          >  1:368007/1‑274 (MQ=255)

                                            ccTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTT‑CTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCAT‑AAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCAt                                                                                                                                                                                                                       <  1:660431/275‑1 (MQ=255)

                                               tCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTT‑CTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCAT‑AAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTTGGCAGAAATACGCACTTCTGGCAGCATCTcgcg                                                                                                                                                                                                                 >  2:448174/1‑278 (MQ=255)

                                               tCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTT‑CTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCAT‑AAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTcgcg                                                                                                                                                                                                                 >  2:14062/1‑278 (MQ=255)

                                               tCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTT‑CTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCAT‑AAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATGGGAATACGCGTGCCGCCTTTCCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGACAGACGCACGTCGGGCAGCAtctc                                                                                                                                                                                                                    >  3:147670/1‑275 (MQ=255)

                                                 aaaCGAATAATGCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCCGCAGGCCGTTTT‑CTTATTTCACCGCTTTCTCGCCAGTGTTCATCAGCAT‑AAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTcgc                                                                                                                                                                                                                  <  3:315805/274‑1 (MQ=255)

                                                    cGCACAGTTGATCTCGGCAGTCGTGACGCAGTGGATGGTGGTCAGCAGGCCGGTTT‑CTCATTTCACCGCTTTCTCGCCAGTGTTCATCAGCAT‑AAAGCAGCCAGTGCCATAGGTGTTCTTCGTCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGCTTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTcgc                                                                                                                                                                                                                  <  3:589548/272‑1 (MQ=255)

                                                            cgactcgtccAGTCGGTCCGCTTCCGATCGTGGTCATGAGTCCGCTTT‑TTCATTTCACCGCGTTCTCGCCAGTTTTCAGCACCAT‑AAAGCAGCCAGTTCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACCACTGTCCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGc                                                                                                                                                                                                    <  4:84775/270‑1 (MQ=255)

                                                              aCTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTT‑CTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCAT‑AAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGCAGCACGACGCACTTCTGGCAGCATCGCGCCCGGCACATCCCGCAc                                                                                                                                                                                                  >  4:110489/1‑278 (MQ=255)

                                                                ttCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTT‑CTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCAT‑AAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACtt                                                                                                                                                                                                >  2:553198/1‑278 (MQ=255)

                                                                 tCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTT‑CTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCAT‑AAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCa                                                                                                                                                                                                   >  1:480878/1‑274 (MQ=255)

                                                                     cAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTT‑CTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCAT‑AAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCAAAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGGGCCGCCCTTGCCGCCAATGTGAGTCTGACCGTAGACTTCGGACGAACGACCAACTTCTGGCAGCAGCCCCGGCGGAATAACCAGCCCTTc                                                                                                                                                                                               >  3:105843/1‑274 (MQ=255)

                                                                                cgggataTGGTCGGCAGTATGCCGTTTT‑CTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCAT‑AAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCA‑TTTTgtcgtc                                                                                                                                                                               <  4:159285/272‑1 (MQ=255)

                                                                                       tggtggTCAGGCAGCCGTTTTCCTGTTTTC‑CCCCTTTCCCGCCCGTGTTCATCAGCAT‑AAAGCAACCCGTGCCATAGGTGTTCCTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCA‑TTTTGTCGTCCCAGTc                                                                                                                                                                         <  2:348352/277‑1 (MQ=255)

                                                                                       tggtggTCAGCAGGCCGTTTT‑CTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCAT‑AAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCA‑TTTTGTCGTCCCAGTc                                                                                                                                                                         <  2:110489/277‑1 (MQ=255)

                                                                                        tgttgtcagaccgCCGTTTC‑CTGATTTGTCCGATTTCTCGCCACTGTTTGTCACCAT‑ATAGCCGCCAGTGCCATAGGTGTTCGTCGCCATCCCTTCTTTCCCCCACAACTGGCCCAACTGCGCGGCCTGCTGTTCACCTGCTAGCACGGGGATTGGAATACGCGTGCCGAGTTTGCCGCCACAGTGAGTCTGACCGTATACTTCGGAAGAACAACGAACTTCTGGCAGAATCTCGCGCGGAATAACCAGCACTTCCAGCA‑TTTTGTCGTCCCAGTCCa                                                                                                                                                                       <  4:253695/266‑1 (MQ=255)

                                                                                         gtggtCAGCAGGCCGTTTT‑CTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCAT‑AAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCA‑TTTTGTCGTCCCAGt                                                                                                                                                                          <  1:723994/274‑1 (MQ=255)

                                                                                          tggaCAGCAGTCCTTTGT‑CTGCTTTCACCGCGTTCTCGCCATTTTTCATCAGCAT‑AAATCAGCCAGTGCCATAGGTGTTCGTCCCCATCCCTTGTTTCACGCACAACTGACCAAACAGCGGGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGTAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCA‑TTTTGTCGTCCCAGTc                                                                                                                                                                         <  3:538235/274‑1 (MQ=255)

                                                                                           ggtCCGCAGGGCGTTTT‑CTGATTTTACCGCTTGCTCCCCAGTGTTCATCAGCAT‑AAAGCAGTCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGTCCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCA‑TTTTGTCGTCCCAGTcc                                                                                                                                                                        <  3:579642/274‑1 (MQ=255)

                                                                                            gtCAGCAGGCCGTTTT‑CTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCAT‑AAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCA‑TTTTGTCGTCCCAGTCCa                                                                                                                                                                       <  1:698700/274‑1 (MQ=255)

                                                                                               agcagGCCGTTTT‑CTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCAT‑AAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCA‑TTTTGTCGTCCCAGTCCAGGGTAt                                                                                                                                                                 <  2:191112/277‑1 (MQ=255)

                                                                                                  cggCCGTTTT‑CTGATTTCACCCCTGTGTCTCCAGTGTTCATCAGCAT‑AAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACGGACCAAACAGCGCGGCCTGCTGGTAACCGGCGATCCCGGAGATTGGAATACGCGGGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCA‑TTTTGTCGTCCCAGTCCAGGGTAt                                                                                                                                                                 <  3:154894/273‑1 (MQ=255)

                                                                                                  agGCCGTTTT‑CTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCAT‑AAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCA‑TTTTGTCGTCCCAGTCCAGGGTAt                                                                                                                                                                 <  3:477915/274‑1 (MQ=255)

                                                                                                   ggcgctttt‑CGCATTTCCTCCCTTTCTCGCCAGTGTTCACCAGCCT‑AAAGCGACCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCCAAACTGACCAAACTGCGCGGCCTCCTGGTCACCGGCGCTCCCGGAGATTGGAATACGAGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCA‑TTTTGTCGTCCCAGTCCAGGGTATGGATg                                                                                                                                                            <  4:409078/273‑1 (MQ=255)

                                                                                                   ggCCGTTTT‑CTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCAT‑AAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGACGCACGACGCACTTCTGGCGGCATCTCGCGCGCAATCTCCAGCACTTCCCCAA‑CTTTGTCGTCCCAGTCCCGGGGCTGGATg                                                                                                                                                            >  4:64833/1‑278 (MQ=255)

                                                                                                    gCCGTTTT‑CTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCAT‑AAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCCCTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTACAGCA‑TTTTGTCGTCCCAGCACAGGGTAGGGATGt                                                                                                                                                           >  2:231780/1‑278 (MQ=255)

                                                                                                      cGTTTT‑CTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCAT‑AAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCA‑TTTTGTCGTCCCAGTCCAGGGTATGGATGTTg                                                                                                                                                         <  2:56992/278‑1 (MQ=255)

                                                                                                              tGATTTCACCGCTTTCTCGCCATTGTTCATCAGCAT‑AAAGCAGCCCGGGCCATAGGTGTTCTTCGCCATCCCTTCTTTCCCTCACAACTGACCCATCAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGGACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCA‑TTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAacaaca                                                                                                                                                  <  2:418249/278‑1 (MQ=255)

                                                                                                                aTTTCACCGCTTTCTCGCCAGTGTTCATCAGCAT‑AAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCA‑TTTTGTCGTCCCAGTCCAGGGTATGGATGTTGaacaa                                                                                                                                                    <  1:340246/274‑1 (MQ=255)

                                                                                                                 tttCACCGCTTTCTCGCCAGTGTTCATCAGCAT‑AAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCA‑TTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACAt                                                                                                                                                 <  2:378598/276‑1 (MQ=255)

                                                                                                                     aCCGCTTTCTCGCCAGTGTTCATCAGCATAAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCA‑TTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTAc                                                                                                                                            >  2:393435/1‑278 (MQ=255)

                                                                                                                       cGCTTTCTCGCCAGTGTTCATCAGCAT‑AAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTcgc                                                                                                                                                                                                                  <  3:662279/206‑1 (MQ=255)

                                                                                                                        gCTTTCTCGCCAGTGTTCATCAGCAT‑AAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCA‑TTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACg                                                                                                                                           >  1:100736/1‑275 (MQ=255)

                                                                                                                          tttCTCGCCAGTGTTCATCAGCAT‑AAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCA‑TTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACgag                                                                                                                                         <  1:607395/275‑1 (MQ=255)

                                                                                                                                 ccAGTGTTCATCAGCAT‑AAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCCGCATCTCGCGCGGCATATCCAGCACTTCCAGCA‑TTTTGTCGTCCCAGTCCAGGGTCTGGATGTTGAATAAATTGGTACGAGAGGAGTTgg                                                                                                                                >  2:30509/1‑277 (MQ=255)

                                                                                                                                         catcaGCAT‑AAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCA‑TTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAAt                                                                                                                          <  1:289916/275‑1 (MQ=255)

                                                                                                                                           ccaGCAT‑GAGGCGGCCCGTGCCAAAGATGTTCTTCGCCATCCCTTCTTTCACCCACAACTGACCAAACAGCGCGGCCTTCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCACGGAATATCCAGCACTTCCAGCA‑TTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCg                                                                                                                        <  3:362867/274‑1 (MQ=255)

                                                                                                                                            caGCAT‑AAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGACGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGGACGAGAGGCGTTGGTGTAATCGGTc                                                                                                                     >  2:442732/1‑278 (MQ=255)

                                                                                                                                                     aggcgcCCGTTCCGTAGGTGTGCCTCGCCATCCCTTCTTTCACCCACAACTGACCAAACAGGGCGGCCTGCTGGTAACCGGCGATCCAGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGGATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCA‑TTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTcaca                                                                                                                  <  3:532351/268‑1 (MQ=255)

                                                                                                                                                     agcagcCAGGGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGGCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCA‑TTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGaca                                                                                                            <  2:226190/278‑1 (MQ=255)

                                                                                                                                                        agcCAGTGCCATGGCGTTTCTGCGCCATCCCTTCTTTCACGCACAACGGACCACACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGGGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCA‑TTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGaca                                                                                                            <  1:17868/275‑1 (MQ=255)

                                                                                                                                                          ccAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCA‑TTTTGTCGTCCCAGTCCAgg                                                                                                                                                                     >  1:475401/1‑216 (MQ=255)

                                                                                                                                                              tGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCA‑TTTTGTCGTCCCAGTCCAGGGTAt                                                                                                                                                                 <  3:475400/216‑1 (MQ=255)

                                                                                                                                                                        gtTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCA‑TTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCAttt                                                                                            >  1:375168/1‑275 (MQ=255)

                                                                                                                                                                           cttcGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGgcagca                                                                                                                                                                                                                        >  4:458525/1‑149 (MQ=255)

                                                                                                                                                                           cttcGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCA‑TTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCGGGGTCATTTTCCCGa                                                                                      >  4:61593/1‑278 (MQ=255)

                                                                                                                                                                                    cccTGCTTTGCCGCCCAACCGAACAAAGGACGGGGCCAGCTGGTCACCGGCGGTCCCGGGGATTGGAATAGGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGAACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCA‑TTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGc                                                                                 <  3:223267/274‑1 (MQ=255)

                                                                                                                                                                                        tcttTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCA‑TTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCAACGt                                                                            >  1:723644/1‑275 (MQ=255)

                                                                                                                                                                                          tttCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCA‑TTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTAt                                                                          >  1:351223/1‑275 (MQ=255)

                                                                                                                                                                                          ttccACGCACAACTAGCCAAACAGCGCGGGCTGCTGGTCACCGGCGAGCCCGGGGATTGTAATACGCGGGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCA‑TTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCaa                                                                       <  4:120307/275‑1 (MQ=255)

                                                                                                                                                                                             cACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCA‑TTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGGGAGGCGTTGTTGTAATCGGTCCCATGGACACGGACCTGAGTCATTTTCCAGATAAGCAActcataaa                                                                       >  1:490736/1‑268 (MQ=255)

                                                                                                                                                                                               cGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCA‑TTTTGTCGTCCCAGTCCCGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGCACACGGCCTTGAGTCCTTTTCCAGATAACCCACGTATCAAccttt                                                                  >  2:633138/1‑275 (MQ=255)

                                                                                                                                                                                               cGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCA‑TTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACCTGGTACGAGATGCGTTGGTGTAATGGGTCACATTGACACGGCCCTGAGTCATTTTCCCGGAAAGCCACGAAttacgcgta                                                                  >  4:146745/1‑270 (MQ=255)

                                                                                                                                                                                               cGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCA‑TTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCCGATAAGCCACGTATCAAcc                                                                     >  1:657376/1‑275 (MQ=255)

                                                                                                                                                                                               cGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCA‑TTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTa                                                                  >  2:50806/1‑278 (MQ=255)

                                                                                                                                                                                                  acacCTGACCAAACAGCGCGGCCGGCTGGTCACCGGCGCTCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCA‑TTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCa                                                               <  2:608342/278‑1 (MQ=255)

                                                                                                                                                                                                     aCTGACCAAACAGCGCGGCCTGCTGGTCACCGGCCATCCCGGCGATTGGAATACTCGGGCCGCCTTTGCCTCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTATGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCA‑TTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCa                                                               <  1:672341/275‑1 (MQ=255)

                                                                                                                                                                                                      cTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCCCGCGCGGAATATCCAGCACTTCCAGCA‑TTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTAcaa                                                               >  1:259975/1‑272 (MQ=255)

                                                                                                                                                                                                              aCAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCA‑TTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTcac                                                                                                                   >  4:248792/1‑214 (MQ=255)

                                                                                                                                                                                                              aCAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCA‑TTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAATCACCGTAACAACCGTACCACACAGCac                                                       >  3:699251/1‑273 (MQ=255)

                                                                                                                                                                                                               cAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCA‑TTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTcaca                                                                                                                  <  2:248792/214‑1 (MQ=255)

                                                                                                                                                                                                                 gcgcGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCA‑TTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCAtt                                                                                             >  1:399764/1‑233 (MQ=255)

                                                                                                                                                                                                                  cgcgGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCTAGCACTTCCAGCA‑TTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGac                                                                                                             >  4:415729/1‑216 (MQ=255)

                                                                                                                                                                                                                  cgcgGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCA‑TTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTca                                                   >  1:460249/1‑274 (MQ=255)

                                                                                                                                                                                                                   gcgGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTcg                                                                                                                                                                                                                   >  1:623807/1‑114 (MQ=255)

                                                                                                                                                                                                                   gcgGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCA‑TTTTGTCGTCCCAGTCCCGGGTATGGATGTTGAACAACATGGTACGAGAGGGGTGGGTGTAATCGGTCACATGGCCACGGCCCTGACTCACTTTCCAATTATGCCACGTATCAACCGTACCaccacgcatttcac                                                  >  3:655135/1‑261 (MQ=255)

                                                                                                                                                                                                                   gcgGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCA‑TTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATTGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCa                                                               <  4:344477/261‑1 (MQ=255)

                                                                                                                                                                                                                     ggCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCA‑TTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTcc                                                                                         <  3:399763/233‑1 (MQ=255)

                                                                                                                                                                                                                       ccTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTcgcgcg                                                                                                                                                                                                               <  3:623806/114‑1 (MQ=255)

                                                                                                                                                                                                                        ctgctgTGCACCGCGGAGACCGGAGATTGGAATACGCTTGCCGCCTTTGCCGACGATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCCCTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCA‑TTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTg                                         <  4:497451/278‑1 (MQ=255)

                                                                                                                                                                                                                                 aCCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCA‑TTTTGTCGTCCCAGTCCAGGGTATGGATGTTGa                                                                                                                                                        >  1:363339/1‑158 (MQ=255)

                                                                                                                                                                                                                                  ccGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCA‑TTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTcgc                                <  4:393435/277‑1 (MQ=255)

                                                                                                                                                                                                                                   cGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCA‑TTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAAAAGCAATTCACCACGACGTGCACGCTc                                  >  3:620286/1‑274 (MQ=255)

                                                                                                                                                                                                                                     gCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCA‑TTTTGTCGTCCCAGTCCAGGGTATGGATGTTGaacaa                                                                                                                                                    <  3:363338/158‑1 (MQ=255)

                                                                                                                                                                                                                                     gCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCACGCGCGGAATATCAAGCACTTCCAGCA‑TTTTGTCGTCCCAGTCCAGGGAATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCACTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGAAATTCACCACGACGTGCACGATCGCgaga                            >  2:41306/1‑278 (MQ=255)

                                                                                                                                                                                                                                      cgttCCCGGACATTGGAATACGCGTGCCGCCTTTGCCTCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCA‑TTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCga                              <  3:460248/272‑1 (MQ=255)

                                                                                                                                                                                                                                        aTCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCA‑TTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGATTCATTTTCCAGATAAGCCACGGATCAACCGTACCAAACAGCAATTCACCAAGACGTGCACGCTCGCgag                             >  1:670344/1‑274 (MQ=255)

                                                                                                                                                                                                                                                  ttGGAATACGCGTGCGGCCTTTGCCGCCAATGGGAGGCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCA‑TTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCcaca                  <  3:375167/275‑1 (MQ=255)

                                                                                                                                                                                                                                                     gAATACGCGTGCCGCCTTTGCCGCCAATGTGAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCA‑TTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCg            <  2:115152/278‑1 (MQ=255)

                                                                                                                                                                                                                                                               gccgccTTTGCCGCAAATGTGAGTCTGACCGTATACGTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCA‑TTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCcact  <  2:61593/278‑1 (MQ=255)

                                                                                                                                                                                                                                                               cccgccTTTGCCGCCAATGGGATGCTGACCGTAAACTTCGGAAGACGGACGCACTTCGGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCA‑TTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCcact  <  4:225026/277‑1 (MQ=255)

CATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTT‑CTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCAT‑AAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCA‑TTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACT  >  NC_000913/4116265‑4116795

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 9 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`